Genomes and Genes
Affiliation: Health Canada
- The role of reverse genetics systems in studying viral hemorrhagic feversHideki Ebihara
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Japan
Thromb Haemost 94:240-53. 2005..Here we review the existing reverse genetics systems for VHF causing viruses and discuss their use in studying viral replication, pathogenesis, and the development of antivirals and vaccines...
- Tyro3 family-mediated cell entry of Ebola and Marburg virusesMasayuki Shimojima
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
J Virol 80:10109-16. 2006..Antibody to Axl inhibited infection of pseudotype viruses in a number of Axl-positive cell lines. These results implicate each Tyro3 family member as a cell entry factor in filovirus infection...
- Molecular determinants of Ebola virus virulence in miceHideki Ebihara
Institute of Medical Science, University of Tokyo, Tokyo, Japan
PLoS Pathog 2:e73. 2006..These findings suggest a critical role for overcoming the interferon-induced antiviral state in the pathogenicity of EBOV and offer new insights into the pathogenesis of EBOV infection...
- In vitro and in vivo characterization of recombinant Ebola viruses expressing enhanced green fluorescent proteinHideki Ebihara
Department of Special Pathogens, International Research Center for Infectious Diseases, University of Tokyo, Tokyo, Japan
J Infect Dis 196:S313-22. 2007..These findings demonstrate that the incorporation of a foreign gene will attenuate ZEBOV in vivo but that these viruses still have potential for in vitro and in vivo research applications...
- Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entryMasayuki Shimojima
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
J Virol 86:2067-78. 2012..The identification of these four molecules as Lassa virus receptors advances our understanding of Lassa virus cell entry...
- Ebola virus matrix protein VP40 uses the COPII transport system for its intracellular transportSeiya Yamayoshi
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo 108 8639, Japan
Cell Host Microbe 3:168-77. 2008..These findings identify a conserved intersection between a host pathway and filovirus replication, an intersection that can be targeted in the development of new antiviral drugs...
- Human macrophage C-type lectin specific for galactose and N-acetylgalactosamine promotes filovirus entryAyato Takada
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
J Virol 78:2943-7. 2004..We propose that filoviruses use different C-type lectins to gain cellular entry, depending on the cell type, and promote efficient viral replication...
- In vitro evaluation of antisense RNA efficacy against filovirus infection, by use of reverse geneticsAllison Groseth
National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada
J Infect Dis 196:S382-9. 2007..Therefore, we explored the application of minigenomes as screening tools to identify functional siRNA targets under biosafety level 2 conditions...
- Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplificationYohei Kurosaki
National Research Institute of Police Science, Kashiwa 277 0882, Japan
J Virol Methods 141:78-83. 2007..Furthermore, as this technique does not require sophisticated instrumentation, it seems very suitable for diagnosis in the field or laboratories in Ebola outbreak areas such as Central Africa...
- Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primatesGabriele Neumann
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Drive, Madison, WI 53706, USA
J Virol 81:2995-8. 2007..We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates...