Research Topics
Genomes and Genes | Fernand GobeilSummaryCountry: Canada Publications
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Publications
PGE(2)-mediated eNOS induction in prolonged hypercapniaDaniella Checchin
Department of Pediatrics, Research Center, , , Montreal, , Canada
Invest Ophthalmol Vis Sci 43:1558-66. 2002..CONCLUSIONS: This study discloses a previously unexplored mechanism for late retinal hyperemia during sustained hypercapnia that appears secondary to the induced expression of eNOS mediated by PGE(2)...
Nuclear prostaglandin signaling system: biogenesis and actions via heptahelical receptorsFernand Gobeil
Department of Pharmacology, Universite de Sherbrooke, 3001, 12th North Avenue, Fleurimont, Sherbrooke, QC J1H 5N4, Canada
Can J Physiol Pharmacol 81:196-204. 2003..The expressional control of G-protein-coupled receptors located at the perinuclear envelope constitutes a novel and distinctive mode of gene regulation...
Modulation of pro-inflammatory gene expression by nuclear lysophosphatidic acid receptor type-1Fernand Gobeil
Departments of Pediatrics, Ophthalmology and Pharmacology, Research Center of Hôpital Sainte Justine, Montreal, Quebec H3T 1C5, Canada
J Biol Chem 278:38875-83. 2003..This study describes for the first time the nucleus as a potential organelle for LPA intracrine signaling in the regulation of pro-inflammatory gene expression...
Nitric oxide signaling via nuclearized endothelial nitric-oxide synthase modulates expression of the immediate early genes iNOS and mPGES-1Fernand Gobeil
Department of Pharmacology, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
J Biol Chem 281:16058-67. 2006..Moreover, our findings strongly support the concept of the nucleus as an autonomous signaling compartment...
G-protein-coupled receptors signalling at the cell nucleus: an emerging paradigmFernand Gobeil
Department of Pharmacology, Faculty of Medicine, Universite de Sherbrooke, Fleurimont, Canada
Can J Physiol Pharmacol 84:287-97. 2006..We will also discuss possible molecular mechanisms responsible for the atypical compartmentalization of GPCRs at the cell nucleus, along with their role in gene expression...
Platelet activating factor receptorsA Marilise Marrache
Research Center, Sainte-Justine Hospital, Montreal, Quebec, Canada
Adv Exp Med Biol 525:161-4. 2003
Intracellular-specific colocalization of prostaglandin E2 synthases and cyclooxygenases in the brainAlejandro Vazquez-Tello
Deptartment of Pediatrics, Research Center of Hôpital Sainte Justine, 3175, Cote Sainte Catherine, Montreal, Quebec, Canada H3T 1C5
Am J Physiol Regul Integr Comp Physiol 287:R1155-63. 2004..Hence, contrary to a previously proposed model of exclusive COX-2/mPGES-1 coordination, COX-2 can coordinate with mPGES-1 and/or cPGES in the brain, depending on the cell compartment and the age group...
Induction of selective blood-tumor barrier permeability and macromolecular transport by a biostable kinin B1 receptor agonist in a glioma rat modelJérôme Côté
Department of Pharmacology, Universite de Sherbrooke, Sherbrooke, Quebec, Canada corrected
PLoS ONE 7:e37485. 2012..They also underlined the potential value of synthetic biostable B1R agonists as selective BTB modulators for local delivery of different sized-therapeutics at (peri)tumoral sites...
New insights into the retinal circulation: inflammatory lipid mediators in ischemic retinopathyPierre Hardy
Department of Obstetrics, Research Center Sainte Justine Hospital and University of Montreal, Montreal, Quebec, Canada, H3T 1C5
Prostaglandins Leukot Essent Fatty Acids 72:301-25. 2005..This review focuses on mechanisms that precede the development of neovascularization, most notably regarding the role of lipid mediators that partake in microvascular degeneration...
Platelet-activating factor in vasoobliteration of oxygen-induced retinopathyMartin Hervé Beauchamp
Department of Pediatrics, Ophthalmology, and Pharmacology, Research Center of Hôpital Ste Justine, Montreal, Quebec, Canada
Invest Ophthalmol Vis Sci 43:3327-37. 2002..To test whether platelet-activating factor (PAF) directly causes retinovascular endothelial cell (EC) death...
Expression of endogenous nuclear bradykinin B2 receptors mediating signaling in immediate early gene activationMartin Savard
Department of Pharmacology, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
J Cell Physiol 216:234-44. 2008..This study discloses a previously undescribed mechanism in BK-induced transcriptional events, via intracrine B2R-mediated signaling, occurring in rat autologous hepatic cells...
Proinflammatory gene induction by platelet-activating factor mediated via its cognate nuclear receptorA Marilise Marrache
Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada
J Immunol 169:6474-81. 2002..This study describes for the first time the nucleus as a putative organelle capable of generating PAF and expresses its receptor, which upon stimulation induces the expression of the proinflammatory gene COX-2...
Novel kinin B1 receptor agonists with improved pharmacological profilesJérôme Côté
Department of Pharmacology, Universite de Sherbrooke, Quebec, Canada
Peptides 30:788-95. 2009..These selective agonists should be valuable in future animal and human studies to investigate the potential benefits of B1R activation...
G-protein-coupled receptors, channels, and Na+-H+ exchanger in nuclear membranes of heart, hepatic, vascular endothelial, and smooth muscle cellsGhassan Bkaily
Department of Anatomy and Cell Biology, Faculty of Medicine, Universite de Sherbrooke, QC, Canada
Can J Physiol Pharmacol 84:431-41. 2006..This article will emphasize these findings...
Angiotensin II induced increase in frequency of cytosolic and nuclear calcium waves of heart cells via activation of AT1 and AT2 receptorsGhassan Bkaily
Department of Anatomy and Cell Biology, Faculty of Medicine, Universite de Sherbrooke, 3001 12th Avenue North, Sherbrooke, Que, Canada J1H 5N4
Peptides 26:1418-26. 2005..These results demonstrate that Ang II, via the activation of its receptors AT1 and AT2, affects the frequency of spontaneous Ca2+ waves and this effect seems to be mediated by the PKC pathway...
Development of a novel noncompetitive antagonist of IL-1 receptorChristiane Quiniou
St Justine Hospital Research Centre, Montreal, Quebec, Canada
J Immunol 180:6977-87. 2008..Findings describe the discovery of a potent and specific small (peptide) antagonist of IL-1RI, with properties in line with an allosteric negative modulator...
Structure-activity relationships of novel peptide agonists of the human bradykinin B2 receptorSimon Belanger
Department of Pharmacology, Universite de Sherbrooke, Quebec, Canada
Peptides 30:777-87. 2009..These novel compounds show promise as candidates for investigating the pharmacology of BK receptors and developing potential therapeutical applications...
Trans-arachidonic acids generated during nitrative stress induce a thrombospondin-1-dependent microvascular degenerationElsa Kermorvant-Duchemin
Department of Pediatrics, Ophthalmology and Pharmacology, Research Center, Hopital Ste Justine, 3175 Côte de Ste Catherine, Montreal, Quebec, H3T1C5, Canada
Nat Med 11:1339-45. 2005....
Altered vascular function in fetal programming of hypertensionDelphine Lamireau
Research Center, , Department of Pediatrics and Pharmacology, , Montreal, Canada
Stroke 33:2992-8. 2002..The study provides an additional explanation for abnormal vasorelaxation in nutrient-deprived subjects in utero...
Bradykinin protects against brain microvascular endothelial cell death induced by pathophysiological stimuliVeronica Bovenzi
Department of Pharmacology, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
J Cell Physiol 222:168-76. 2010..The present study proposes a novel role for BK in microvascular endothelial protection, which may be pertinent to the complex mechanism of action of ACEi explaining their long-term beneficial effects in maintaining vascular integrity...
Selective tumor blood-brain barrier opening with the kinin B2 receptor agonist [Phe(8)psi(CH(2)NH)Arg(9)]-BK in a F98 glioma rat model: an MRI studyJérôme Côté
Department of Nuclear Medicine and Radiobiology, Universite de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4
Neuropeptides 44:177-85. 2010..We concluded that R523 allows the extravasation of hydrophilic macromolecular agents (17kDa) into tumor tissues by inducing selective tumor BBB permeability via B2R- and NO-dependent mechanisms...
Effect of thrombin and bradykinin on endothelial cell mechanical properties monitored through membrane deformationCharles M Cuerrier
Departement de Pharmacologie, Universite de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada
J Mol Recognit 22:389-96. 2009..These changes in the mechanical properties of endothelial cells provide relevant informations in the biological and pathophysiological behaviors of endothelial cells...
Nitric oxide and reactive oxygen species in the nucleus revisitedChantale Provost
Department of Anatomy and Cell Biology, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Can J Physiol Pharmacol 88:296-304. 2010..These results highly suggest that ROS formation is also generated directly by the nucleus and that free radicals may contribute to ET-1 regulation of nuclear Ca2+ homeostasis...
Hyperoxic exposure leads to nitrative stress and ensuing microvascular degeneration and diminished brain mass and function in the immature subjectMirna Sirinyan
Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada
Stroke 37:2807-15. 2006..CONCLUSIONS: Data identify NO and nitrating agents as major mediators of cerebral microvascular damage, ensuing impaired brain development and function in immature subjects exposed to hyperoxia...
Lipopolysaccharide and hypoxia/ischemia induced IL-2 expression by microglia in neonatal brainSylvie Girard
Child Neurology Laboratory, Department of Pediatrics, Universite de Sherbrooke, Quebec, Canada
Neuroreport 19:997-1002. 2008..These results disclosed the ability of microglia to produce IL-2 and also suggest the implication of IL-2 in neural cell death triggered by perinatal lipopolysaccharide and hypoxia/ischemia exposures...
Intracellular signaling of lipid mediators via cognate nuclear G protein-coupled receptorsAnne Marilise Marrache
Department of Pediatrics, , Montreal, Quebec, Canada
Endothelium 12:63-72. 2005..Intracrine signaling for lipid mediators uncover novel pathways to elicit their effects; moreover, intracellular GPCRs constitute a distinctive mode of action for gene regulation...
Urotensin II-induced hypotensive responses in Wistar-Kyoto (Wky) and spontaneously hypertensive (Shr) ratsGabrielle Gendron
Department of Pharmacology, Faculty of Medicine, , Sherbrooke, Canada J1H 5N4
Peptides 26:1468-74. 2005....
Contractile responses of aortae from WKY and SHR to vasoconstrictorsGabrielle Gendron
Department of Pharmacology, University of Sherbrooke, Sherbrooke, Quebec, Canada
Clin Exp Hypertens 26:511-23. 2004....
Hypotensive effects of hemopressin and bradykinin in rabbits, rats and mice. A comparative studyPaul André Blais
Faculty of Medicine, Department of Pharmacology, Universite de Sherbrooke, 301 12th North Avenue, Fleurimont, Sherbrooke, Que, Canada J1H 5N4
Peptides 26:1317-22. 2005..Therefore, HP acts as a weak hypotensive mediator, which does not activate kinin B2 receptors, but uses a functional site and/or signaling paths appearing to be up-regulated by LPS...
Ascorbic acid decreases the binding affinity of the AT1 receptor for angiotensin IIPatrice C Leclerc
Department of Pharmacology, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Quebec, Canada
Am J Hypertens 21:67-71. 2008..We therefore hypothesized that ascorbic acid could modify the pharmacological properties of the AT(1) receptor for Ang II...
Real-time monitoring of angiotensin II-induced contractile response and cytoskeleton remodeling in individual cells by atomic force microscopyCharles M Cuerrier
Departement de Pharmacologie, Universite de Sherbrooke, 3001, 12e Avenue Nord, Sherbrooke, QC J1H 5N4, Canada
Pflugers Arch 457:1361-72. 2009....
Enzymatic pathways involved in the generation of endothelin-1(1-31) from exogenous big endothelin-1 in the rabbit aortaCarlos R Tirapelli
Department of Pharmacology, Faculty of Medicine and Health Sciences, , , Canada
Br J Pharmacol 148:527-35. 2006..Finally, we showed that NEP is the predominant enzymatic pathway involved in the cleavage of ET-1(1-31) to a bioactive metabolite that will act on ET(A) receptors to induce contraction in the rabbit aorta...
Pro-inflammatory disequilibrium of the IL-1 beta/IL-1ra ratio in an experimental model of perinatal brain damages induced by lipopolysaccharide and hypoxia-ischemiaSylvie Girard
Laboratoire de Neuropédiatrie, Departement de Pediatrie, Universite de Sherbrooke, 3001 12eme Avenue Nord, Sherbrooke, Que, Canada
Cytokine 43:54-62. 2008..The resulting pro-inflammatory orientation in the IL-1/IL-1ra balance might play a role in the initiation of perinatal brain damages...
Immunofluorescence revealed the presence of NHE-1 in the nuclear membranes of rat cardiomyocytes and isolated nuclei of human, rabbit, and rat aortic and liver tissuesGhassan Bkaily
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Sherbrooke, 3001 12th Avenue North, Sherbrooke, Quebec J1H 5N4, Canada
Can J Physiol Pharmacol 82:805-11. 2004..These results suggest that nuclear membranes' NHE-1 may play a role in the modulation of intranuclear pH...
Human urotensin-II enhances plasma extravasation in specific vascular districts in Wistar ratsGabrielle Gendron
Institute of Pharmacology, Faculty of Medicine, Universite de Sherbrooke, 3001 12th Avenue Nord, Sherbrooke, Quebec J1K 2R1, Canada
Can J Physiol Pharmacol 82:16-21. 2004..The present study is the first report describing the modulator roles of U-II on vascular permeability in specific organs. Moreover, the action of U-II appears specific, since it is independent of the ET-1 and PAF signalling pathways...
Kinin B1 receptor antagonists with multi-enzymatic resistance propertiesWitold Neugebauer
Institute of Pharmacology, Universite de Sherbrooke, QC, Canada
Can J Physiol Pharmacol 80:287-92. 2002..g., Lys[Leu8]desArg9BK) and more recent (e.g., R-715, B-9858) generations of B, receptor antagonists. Ongoing in vivo assays will validate the assumption that the analogues R-954 and R-955 have a prolonged duration of action...
Studies on the angiotensin-converting enzyme and the kinin B2 receptor in the rabbit jugular vein: modulation of contractile response to bradykininFernand Gobeil
Department of Pharmacology, Medical School, , QC, Canada
Can J Physiol Pharmacol 80:153-63. 2002....
Regulation of eNOS expression in brain endothelial cells by perinuclear EP(3) receptorsFernand Gobeil
Departments of Pediatrics, ,
Circ Res 90:682-9. 2002....
Agonist-independent nuclear localization of the Apelin, angiotensin AT1, and bradykinin B2 receptorsDennis K Lee
Department of Pharmacology, University of Toronto, Toronto, Ontario M5S 1A8, USA
J Biol Chem 279:7901-8. 2004..This discovery of apelin, AT(1), and B(2) receptors with agonist-independent nuclear translocation suggests major unanticipated roles for these receptors in cell signaling and function...
Do angiotensin-converting enzyme inhibitors directly stimulate the kinin B1 receptor?Jean Philippe Fortin
Centre de recherche en cancérologie de l Université Laval, Centre Hospitalier Universitaire de Quebec, Centre de recherche du Pavillon l Hôtel Dieu de Québec, Canada G1R 2J6
Am J Physiol Heart Circ Physiol 285:H277-82. 2003..The phospho-ERK1/2 content of arterial smooth muscle cells of human or rabbit origin was increased by treatment with Lys-des-Arg9-BK but not with enalaprilat. ACE inhibitors do not act as bona fide agonists of the kinin B1 receptors...
Dominant role for calpain in thromboxane-induced neuromicrovascular endothelial cytotoxicityChristiane Quiniou
Department of Pediatrics, , , , Canada
J Pharmacol Exp Ther 316:618-27. 2006..Overall, this work identifies (specifically) m-calpain as a dominant protease in TXA(2)-induced neurovascular endothelial cell death...
Selective neuromicrovascular endothelial cell death by 8-Iso-prostaglandin F2alpha: possible role in ischemic brain injurySonia Brault
, Department of Pediatrics and Pharmacology, , , , Canada
Stroke 34:776-82. 2003..CONCLUSIONS: These data disclose a novel action of 8-iso-PGF2alpha involving TXA2 in oxidant stress-induced cerebral microvascular injury and brain damage...
Redox-dependent effects of nitric oxide on microvascular integrity in oxygen-induced retinopathyMartin H Beauchamp
Department of Pediatrics, Ophthalmology, and Pharmacology, , , , Canada
Free Radic Biol Med 37:1885-94. 2004..Data disclose that the dual effects of NO on newborn retinal microvascular integrity in response to hyperoxia in vivo depend on the redox state and seem mediated at least in part by VEGFR-2...
Intracrine signaling through lipid mediators and their cognate nuclear G-protein-coupled receptors: a paradigm based on PGE2, PAF, and LPA1 receptorsTang Zhu
Department of Pediatrics, , , QC, Canada
Can J Physiol Pharmacol 84:377-91. 2006..Intracrine signaling for lipid mediators uncover novel pathways to elicit their effects; accordingly, intracellular GPCRs constitute a distinctive mode of action for gene regulation...
Isomer-specific contractile effects of a series of synthetic f2-isoprostanes on retinal and cerebral microvasculatureXin Hou
, Department of Pediatrics and Department of Pharmacology, , , PQ, H3T 1C5, , Canada
Free Radic Biol Med 36:163-72. 2004..These new findings broaden the scope of our understanding regarding the potential involvement of F2-IsoP's as mediators of oxidant injury...
Increased platelet-activating factor-induced periventricular brain microvascular constriction associated with immaturityXin Hou
, Department of Pediatrics and Pharmacology, , , H3T 1C5
Am J Physiol Regul Integr Comp Physiol 284:R928-35. 2003..The resulting decrease in blood flow may contribute to the increased vulnerability of the PV brain regions to oxidant stress-induced injury in immature subjects...
Lysophosphatidic acid induces endothelial cell death by modulating the redox environmentSonia Brault
Department of Pediatrics, Research Center, Hopital Sainte Justine, 3175 Cote Sainte Catherine, Montreal, Quebec, Canada H3T 1C5
Am J Physiol Regul Integr Comp Physiol 292:R1174-83. 2007..Data implicate novel properties for LPA as a modulator of the cell redox environment, which partakes in endothelial cell death and ensued neuromicrovascular rarefaction...
