Andres Finzi

Summary

Country: Canada

Publications

  1. Lee W, Richard J, Lichtfuss M, Smith A, Park J, Courter J, et al. Antibody-Dependent Cellular Cytotoxicity against Reactivated HIV-1-Infected Cells. J Virol. 2016;90:2021-30 pubmed publisher
    ..Our study highlights the potential role that antibody-dependent cellular cytotoxicity might play in antilatency cure approaches. ..
  2. Richard J, Veillette M, Brassard N, Iyer S, Roger M, Martin L, et al. CD4 mimetics sensitize HIV-1-infected cells to ADCC. Proc Natl Acad Sci U S A. 2015;112:E2687-94 pubmed publisher
    ..Thus, CD4 mimetics hold the promise of therapeutic utility in preventing and controlling HIV-1 infection. ..
  3. Ding S, Tolbert W, Prévost J, Pacheco B, Coutu M, Debbeche O, et al. A Highly Conserved gp120 Inner Domain Residue Modulates Env Conformation and Trimer Stability. J Virol. 2016;90:8395-409 pubmed publisher
    ..Here, we show that W69, located at the interface between gp120 and gp41 in the PGT151-bound trimer, plays a critical role in the interprotomer signaling induced by CD4 binding. This new information might be useful in immunogen design. ..
  4. Alsahafi N, Bakouche N, Kazemi M, Richard J, Ding S, Bhattacharyya S, et al. An Asymmetric Opening of HIV-1 Envelope Mediates Antibody-Dependent Cellular Cytotoxicity. Cell Host Microbe. 2019;25:578-587.e5 pubmed publisher
    ..Additionally, triggering State 2A on HIV-infected primary CD4+ T cells exposes epitopes that induce ADCC. Strategies that induce this Env conformation may represent approaches to fight HIV-1 infection. ..
  5. Anand S, Grover J, Tolbert W, Prévost J, Richard J, Ding S, et al. Antibody-induced internalization of HIV-1 Env proteins limits the surface expression of the closed conformation of Env. J Virol. 2019;: pubmed publisher
    ..Thus a better understanding this mechanism might help develop antibodies with improved capacities to mediate ADCC. ..
  6. Anand S, Prévost J, Baril S, Richard J, Medjahed H, Chapleau J, et al. Two Families of Env Antibodies Efficiently Engage Fc-Gamma Receptors and Eliminate HIV-1-Infected Cells. J Virol. 2019;93: pubmed publisher
    ..Here we report that the binding of these two families of antibodies is required to engage FcγRIIIa and mediate ADCC. ..
  7. Alsahafi N, Anand S, Castillo Menendez L, Verly M, Medjahed H, Prévost J, et al. SOSIP Changes Affect Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Conformation and CD4 Engagement. J Virol. 2018;92: pubmed publisher
    ..Our studies underscore the need to acquire structural information on the pretriggered Env conformation, which is recognized by most broadly reactive neutralizing antibodies. ..
  8. Richard J, Prévost J, Baxter A, von Bredow B, Ding S, Medjahed H, et al. Uninfected Bystander Cells Impact the Measurement of HIV-Specific Antibody-Dependent Cellular Cytotoxicity Responses. MBio. 2018;9: pubmed publisher
    ..Our results strongly argue for the use of assays that measure ADCC against HIV-1-infected cells expressing physiologically relevant conformations of Env to evaluate correlates of protection in vaccine trials. ..
  9. Coutu M, Finzi A. HIV-1 gp120 dimers decrease the overall affinity of gp120 preparations for CD4-induced ligands. J Virol Methods. 2015;215-216:37-44 pubmed publisher
    ..Removal of these aberrant disulfide-linked gp120 dimers by standard size exclusion chromatography is sufficient to restore the overall affinity of gp120 preparations for these ligands. ..

More Information

Publications24

  1. Veillette M, Coutu M, Richard J, Batraville L, Désormeaux A, Roger M, et al. Conformational evaluation of HIV-1 trimeric envelope glycoproteins using a cell-based ELISA assay. J Vis Exp. 2014;:51995 pubmed publisher
    ..It requires minimal amount of material and no highly-specialized equipment or know-how. Thus, this technique can be established for medium to high throughput screening of antigens and antibodies, such as newly-isolated antibodies. ..
  2. Lewis G, Finzi A, DeVico A, Pazgier M. Conformational Masking and Receptor-Dependent Unmasking of Highly Conserved Env Epitopes Recognized by Non-Neutralizing Antibodies That Mediate Potent ADCC against HIV-1. Viruses. 2015;7:5115-32 pubmed publisher
    ..Here, we discuss a less studied problem, the structural basis of protection (and its evasion) by antibodies that protect only by potent Fc-mediated effector function. ..
  3. Alsahafi N, Ding S, Richard J, Markle T, Brassard N, Walker B, et al. Nef Proteins from HIV-1 Elite Controllers Are Inefficient at Preventing Antibody-Dependent Cellular Cytotoxicity. J Virol. 2015;90:2993-3002 pubmed publisher
    ..Therefore, targeting Nef's ability to downregulate CD4 could render HIV-1-infected cells susceptible to ADCC and thus have therapeutic utility. ..
  4. Ding S, Medjahed H, Prévost J, Coutu M, Xiang S, Finzi A. Lineage-Specific Differences between the gp120 Inner Domain Layer 3 of Human Immunodeficiency Virus and That of Simian Immunodeficiency Virus. J Virol. 2016;90:10065-10073 pubmed publisher
    ..Here we report that SIV layer 3 has a greater impact on CD4 binding than does layer 3 in HIV-1 gp120. This work defines lineage-specific differences in layer 3 from HIV-1 and SIV. ..
  5. Richard J, Veillette M, Ding S, Zoubchenok D, Alsahafi N, Coutu M, et al. Small CD4 Mimetics Prevent HIV-1 Uninfected Bystander CD4 + T Cell Killing Mediated by Antibody-dependent Cell-mediated Cytotoxicity. EBioMedicine. 2016;3:122-134 pubmed publisher
  6. Alsahafi N, Richard J, Prévost J, Coutu M, Brassard N, Parsons M, et al. Impaired Downregulation of NKG2D Ligands by Nef Proteins from Elite Controllers Sensitizes HIV-1-Infected Cells to Antibody-Dependent Cellular Cytotoxicity. J Virol. 2017;91: pubmed publisher
    ..We now show that downregulation of NKG2D ligands by HIV-1 Nef from EC is inefficient and leaves infected cells susceptible to ADCC. These data suggest a critical role for NKG2D ligands in anti-HIV-1 ADCC responses. ..
  7. Veillette M, Coutu M, Richard J, Batraville L, Dagher O, Bernard N, et al. The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals. J Virol. 2015;89:545-51 pubmed publisher
    ..This observation suggests that HIV-1 developed sophisticated mechanisms to minimize the exposure of these epitopes at the surface of infected cells. ..
  8. Prévost J, Zoubchenok D, Richard J, Veillette M, Pacheco B, Coutu M, et al. Influence of the Envelope gp120 Phe 43 Cavity on HIV-1 Sensitivity to Antibody-Dependent Cell-Mediated Cytotoxicity Responses. J Virol. 2017;91: pubmed publisher
    ..CRF01_AE HIV-1 strains have an unusual Phe 43 cavity-filling His 375 residue, which increases the propensity of Env to sample the CD4-bound conformation, thereby increasing susceptibility to ADCC. ..
  9. Ding S, Veillette M, Coutu M, Prévost J, Scharf L, Bjorkman P, et al. A Highly Conserved Residue of the HIV-1 gp120 Inner Domain Is Important for Antibody-Dependent Cellular Cytotoxicity Responses Mediated by Anti-cluster A Antibodies. J Virol. 2016;90:2127-34 pubmed publisher
    ..Here, we show that a highly conserved tryptophan at position 69 of the gp120 inner domain is important for ADCC mediated by anti-cluster A antibodies and sera from HIV-1-infected individuals. ..
  10. request reprint
    Veillette M, Richard J, Pazgier M, Lewis G, Parsons M, Finzi A. Role of HIV-1 Envelope Glycoproteins Conformation and Accessory Proteins on ADCC Responses. Curr HIV Res. 2016;14:9-23 pubmed
  11. Richard J, Prévost J, Alsahafi N, Ding S, Finzi A. Impact of HIV-1 Envelope Conformation on ADCC Responses. Trends Microbiol. 2018;26:253-265 pubmed publisher
    ..Cells exposing State 1 Env are susceptible to ADCC mediated by bNAbs. Here, we discuss how Env conformation affects ADCC responses and in vitro measurements. ..
  12. Zoubchenok D, Veillette M, Prévost J, Sanders Buell E, Wagh K, Korber B, et al. Histidine 375 Modulates CD4 Binding in HIV-1 CRF01_AE Envelope Glycoproteins. J Virol. 2017;91: pubmed publisher
    ..Moreover, this work suggests that specific residues of the gp120 inner domain layers have coevolved with H375 in order to maintain its ability to mediate viral entry. ..
  13. Richard J, Pacheco B, Gohain N, Veillette M, Ding S, Alsahafi N, et al. Co-receptor Binding Site Antibodies Enable CD4-Mimetics to Expose Conserved Anti-cluster A ADCC Epitopes on HIV-1 Envelope Glycoproteins. EBioMedicine. 2016;12:208-218 pubmed publisher
    ..The understanding of the conformational changes required to expose these vulnerable Env epitopes might be important in the design of new strategies aimed at fighting HIV-1. ..
  14. Prévost J, Richard J, Ding S, Pacheco B, Charlebois R, Hahn B, et al. Envelope glycoproteins sampling states 2/3 are susceptible to ADCC by sera from HIV-1-infected individuals. Virology. 2018;515:38-45 pubmed publisher
    ..Our results indicate that the conformations spontaneously sampled by the Env trimer at the surface of infected cells has a significant impact on ADCC responses. ..
  15. Richard J, Ding S, Finzi A. Unlocking HIV-1 Env: implications for antibody attack. AIDS Res Ther. 2017;14:42 pubmed publisher