Research Topics
| Doug CrumpSummaryAffiliation: Environment Canada Country: Canada Publications
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Detail Information
Publications
Effects of tris(1,3-dichloro-2-propyl) phosphate and tris(1-chloropropyl) phosphate on cytotoxicity and mRNA expression in primary cultures of avian hepatocytes and neuronal cellsDoug Crump
Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1A 0H3
Toxicol Sci 126:140-8. 2012..The mRNA expression data were similar to those from a previous study with hexabromocyclododecane...- Pipping success, isomer-specific accumulation, and hepatic mRNA expression in chicken embryos exposed to HBCDDoug Crump
Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
Toxicol Sci 115:492-500. 2010..Research on the effects of HBCD in wild avian species is warranted... - Detection of PBDE effects on mRNA expression in chicken (Gallus domesticus) neuronal cells using real-time RT-PCR and a new differential display methodDoug Crump
Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6
Toxicol In Vitro 22:1337-43. 2008..Taken together, the findings from this study indicate that this in vitro cell culture method can be used to characterize the effects of PBDEs in the avian brain... - Effects of hexabromocyclododecane and polybrominated diphenyl ethers on mRNA expression in chicken (Gallus domesticus) hepatocytesDoug Crump
Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada
Toxicol Sci 106:479-87. 2008.... 
Detection of polybrominated diphenyl ethers in herring gull (Larus argentatus) brains: effects on mRNA expression in cultured neuronal cellsDoug Crump
Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
Environ Sci Technol 42:7715-21. 2008..Herring gull neuronal cells were responsive to both T3 and PBDEs although, receptors associated with two predicted mechanisms of PBDE action were not effective molecular biomarkers of exposure...- The effects of Dechlorane Plus on toxicity and mRNA expression in chicken embryos: a comparison of in vitro and in ovo approachesDoug Crump
Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
Comp Biochem Physiol C Toxicol Pharmacol 154:129-34. 2011..At current environmental exposure levels, no adverse effects of DP on embryonic viability or pathways associated with the genes assessed are predicted... - In vitro and in ovo effects of four brominated flame retardants on toxicity and hepatic mRNA expression in chicken embryosCaroline Egloff
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario, Canada K1N6N5
Toxicol Lett 207:25-33. 2011..None of the gene targets were responsive to BEHTBP exposure in CEH. The multi-tiered in vitro/in ovo screening approach was effective for assessing toxicological and molecular biological effects of these BFRs in an avian species... - Induction of cytochrome P4501A by highly purified hexachlorobenzene in primary cultures of ring-necked pheasant and Japanese quail embryo hepatocytesLukas J Mundy
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
Comp Biochem Physiol C Toxicol Pharmacol 155:498-505. 2012.... - In ovo effects of perfluorohexane sulfonate and perfluorohexanoate on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryosCristina G Cassone
Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
Toxicol Sci 127:216-24. 2012..This in ovo study successfully validated previous in vitro results concerning the modulation of TH-responsive genes and identified adverse effects associated with TH homeostasis in response to PFHxS treatment... - Ethoxyresorufin O-deethylase induction by TCDD, PeCDF and TCDF in ring-necked pheasant and Japanese quail hepatocytes: Time-dependent effects on concentration-response curvesJessica C Hervé
Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
Toxicol In Vitro 24:1301-5. 2010.... - Technical-grade perfluorooctane sulfonate alters the expression of more transcripts in cultured chicken embryonic hepatocytes than linear perfluorooctane sulfonateJason M O'Brien
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
Environ Toxicol Chem 30:2846-59. 2011..These data show that T-PFOS elicits a greater transcriptional response in CEH than L-PFOS alone and demonstrates the importance of considering the isomer-specific toxicological properties of PFOS when assessing exposure risk... - Sequence and in vitro function of chicken, ring-necked pheasant, and Japanese quail AHR1 predict in vivo sensitivity to dioxinsReza Farmahin
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
Environ Sci Technol 46:2967-75. 2012..Our results support and expand previous in vitro and in vivo work that demonstrated ligand-dependent species differences in AHR1 affinity. The findings and methods will be of use for DLC risk assessments... 
Effects of perfluoroalkyl compounds on mRNA expression levels of thyroid hormone-responsive genes in primary cultures of avian neuronal cellsViengtha Vongphachan
Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
Toxicol Sci 120:392-402. 2011..This is the first study to report that PFC exposure alters mRNA expression in primary cultures of avian neuronal cells and may provide insight into the possible mechanisms of action of PFCs in the avian brain...- Highly purified hexachlorobenzene induces cytochrome P4501A in primary cultures of chicken embryo hepatocytesLukas J Mundy
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
Toxicol Appl Pharmacol 248:185-93. 2010..The maximal EROD activity and CYP1A4/5 mRNA expression differed greatly between HCB and TCDD, and may contribute to an overestimation of the ReP value calculated for highly purified HCB... - Pipping success and liver mRNA expression in chicken embryos exposed in ovo to C8 and C11 perfluorinated carboxylic acids and C10 perfluorinated sulfonateJason M O'Brien
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario K1N 6N5, Canada
Toxicol Lett 190:134-9. 2009..Based on the present results for white leghorn chickens, current environmental concentrations of PFOA, PFUdA and PFDS are unlikely to affect the hatching success of wild birds... - Transcriptional profiles in the cerebral hemisphere of chicken embryos following in ovo perfluorohexane sulfonate exposureCristina G Cassone
Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
Toxicol Sci 129:380-91. 2012.... - Amino acid sequence of the ligand-binding domain of the aryl hydrocarbon receptor 1 predicts sensitivity of wild birds to effects of dioxin-like compoundsReza Farmahin
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
Toxicol Sci 131:139-52. 2013..93, p < 0.0001) with in ovo toxicity data for those species. These results indicate promise for the use of AHR1 LBD amino acid sequences independently, or combined with the LRG assay, to predict avian species sensitivity to DLCs... - Cytochrome P4501A induction in avian hepatocyte cultures exposed to polychlorinated biphenyls: comparisons with AHR1-mediated reporter gene activity and in ovo toxicityGillian E Manning
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
Toxicol Appl Pharmacol 266:38-47. 2013..Relative potency values were generally similar between the LRG and EROD assays and indicate that the relative potency of some PCBs may differ among species... - Fluorescent RNA arbitrarily primed polymerase chain reaction. A new differential display approach to detect contaminant-induced alterations of gene expression in wildlife speciesDoug Crump
National Wildlife Research Center, Canadian Wildlife Service, Carleton University, Ottawa, Ontario, Canada
Methods Mol Biol 410:15-27. 2008..g., cultured cells, tissue). This straightforward, safe, and cost-effective approach represents a useful alternative to the radiometric-based RAP-PCR method... - Effects of 18 perfluoroalkyl compounds on mRNA expression in chicken embryo hepatocyte culturesNathan J Hickey
Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario K1A 0H3, Canada
Toxicol Sci 111:311-20. 2009..This study is the first to expose CEH cultures to multiple PFCs in vitro and demonstrates that exposure to PFC solutions of different isomeric content or chain length causes variable transcriptional responses... - Cytochrome P4501A induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin and two chlorinated dibenzofurans in primary hepatocyte cultures of three avian speciesJessica C Hervé
Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ontario, Canada
Toxicol Sci 113:380-91. 2010..This study indicates that PeCDF is more potent than TCDD in ring-necked pheasant and Japanese quail hepatocytes and that ring-necked pheasant, Japanese quail, and chicken hepatocytes are equally sensitive to CYP1A induction by PeCDF... - Cytochrome P4501A induction in primary cultures of embryonic European starling hepatocytes exposed to TCDD, PeCDF and TCDFReza Farmahin
Centre for Advanced Research in Environmental Genomics Department of Biology, University of Ottawa, Ottawa, ON, K1N 6N5, Canada
Ecotoxicology 22:731-9. 2013..However, starling hepatocytes are less sensitive than chicken hepatocytes to CYP1A4/5 induction by PeCDF and TCDF... - A luciferase reporter gene assay and aryl hydrocarbon receptor 1 genotype predict the LD50 of polychlorinated biphenyls in avian speciesGillian E Manning
Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
Toxicol Appl Pharmacol 263:390-401. 2012.... - Expression profiles of novel thyroid hormone-responsive genes and proteins in the tail of Xenopus laevis tadpoles undergoing precocious metamorphosisCaren C Helbing
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada V8W 3P6
Mol Endocrinol 17:1395-409. 2003.... - Exposure to the herbicide acetochlor alters thyroid hormone-dependent gene expression and metamorphosis in Xenopus LaevisDoug Crump
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
Environ Health Perspect 110:1199-205. 2002..The morphologic changes observed are preceded by alterations in gene expression profiles detected in the tadpole tail, and the nature of these profiles suggest a novel mechanism of action for acetochlor... - Distinctive gene profiles occur at key points during natural metamorphosis in the Xenopus laevis tadpole tailNik Veldhoen
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
Dev Dyn 225:457-68. 2002..This work establishes an important baseline for determining the mechanisms whereby tissues undergo differing metamorphic fates...