Doug Crump

Summary

Affiliation: Environment Canada
Country: Canada

Publications

  1. ncbi request reprint 1,2-Dibromo-4-(1,2-dibromoethyl)-cyclohexane and tris(methylphenyl) phosphate cause significant effects on development, mRNA expression, and circulating bile acid concentrations in chicken embryos
    Doug Crump
    National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3, Canada Electronic address
    Toxicol Appl Pharmacol 277:279-87. 2014
  2. doi request reprint Effects of tris(1,3-dichloro-2-propyl) phosphate and tris(1-chloropropyl) phosphate on cytotoxicity and mRNA expression in primary cultures of avian hepatocytes and neuronal cells
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 126:140-8. 2012
  3. doi request reprint Pipping success, isomer-specific accumulation, and hepatic mRNA expression in chicken embryos exposed to HBCD
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 115:492-500. 2010
  4. ncbi request reprint Detection of polybrominated diphenyl ethers in herring gull (Larus argentatus) brains: effects on mRNA expression in cultured neuronal cells
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
    Environ Sci Technol 42:7715-21. 2008
  5. doi request reprint Effects of hexabromocyclododecane and polybrominated diphenyl ethers on mRNA expression in chicken (Gallus domesticus) hepatocytes
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada
    Toxicol Sci 106:479-87. 2008
  6. ncbi request reprint Detection of PBDE effects on mRNA expression in chicken (Gallus domesticus) neuronal cells using real-time RT-PCR and a new differential display method
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6
    Toxicol In Vitro 22:1337-43. 2008
  7. doi request reprint The effects of Dechlorane Plus on toxicity and mRNA expression in chicken embryos: a comparison of in vitro and in ovo approaches
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 154:129-34. 2011
  8. doi request reprint Amino acid sequence of the ligand-binding domain of the aryl hydrocarbon receptor 1 predicts sensitivity of wild birds to effects of dioxin-like compounds
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    Toxicol Sci 131:139-52. 2013
  9. doi request reprint In ovo effects of perfluorohexane sulfonate and perfluorohexanoate on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryos
    Cristina G Cassone
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 127:216-24. 2012
  10. doi request reprint In vitro and in ovo effects of four brominated flame retardants on toxicity and hepatic mRNA expression in chicken embryos
    Caroline Egloff
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario, Canada K1N6N5
    Toxicol Lett 207:25-33. 2011

Collaborators

Detail Information

Publications32

  1. ncbi request reprint 1,2-Dibromo-4-(1,2-dibromoethyl)-cyclohexane and tris(methylphenyl) phosphate cause significant effects on development, mRNA expression, and circulating bile acid concentrations in chicken embryos
    Doug Crump
    National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3, Canada Electronic address
    Toxicol Appl Pharmacol 277:279-87. 2014
    ....
  2. doi request reprint Effects of tris(1,3-dichloro-2-propyl) phosphate and tris(1-chloropropyl) phosphate on cytotoxicity and mRNA expression in primary cultures of avian hepatocytes and neuronal cells
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 126:140-8. 2012
    ..The mRNA expression data were similar to those from a previous study with hexabromocyclododecane...
  3. doi request reprint Pipping success, isomer-specific accumulation, and hepatic mRNA expression in chicken embryos exposed to HBCD
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 115:492-500. 2010
    ..Research on the effects of HBCD in wild avian species is warranted...
  4. ncbi request reprint Detection of polybrominated diphenyl ethers in herring gull (Larus argentatus) brains: effects on mRNA expression in cultured neuronal cells
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
    Environ Sci Technol 42:7715-21. 2008
    ..Herring gull neuronal cells were responsive to both T3 and PBDEs although, receptors associated with two predicted mechanisms of PBDE action were not effective molecular biomarkers of exposure...
  5. doi request reprint Effects of hexabromocyclododecane and polybrominated diphenyl ethers on mRNA expression in chicken (Gallus domesticus) hepatocytes
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada
    Toxicol Sci 106:479-87. 2008
    ....
  6. ncbi request reprint Detection of PBDE effects on mRNA expression in chicken (Gallus domesticus) neuronal cells using real-time RT-PCR and a new differential display method
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6
    Toxicol In Vitro 22:1337-43. 2008
    ..Taken together, the findings from this study indicate that this in vitro cell culture method can be used to characterize the effects of PBDEs in the avian brain...
  7. doi request reprint The effects of Dechlorane Plus on toxicity and mRNA expression in chicken embryos: a comparison of in vitro and in ovo approaches
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 154:129-34. 2011
    ..At current environmental exposure levels, no adverse effects of DP on embryonic viability or pathways associated with the genes assessed are predicted...
  8. doi request reprint Amino acid sequence of the ligand-binding domain of the aryl hydrocarbon receptor 1 predicts sensitivity of wild birds to effects of dioxin-like compounds
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    Toxicol Sci 131:139-52. 2013
    ..93, p < 0.0001) with in ovo toxicity data for those species. These results indicate promise for the use of AHR1 LBD amino acid sequences independently, or combined with the LRG assay, to predict avian species sensitivity to DLCs...
  9. doi request reprint In ovo effects of perfluorohexane sulfonate and perfluorohexanoate on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryos
    Cristina G Cassone
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 127:216-24. 2012
    ..This in ovo study successfully validated previous in vitro results concerning the modulation of TH-responsive genes and identified adverse effects associated with TH homeostasis in response to PFHxS treatment...
  10. doi request reprint In vitro and in ovo effects of four brominated flame retardants on toxicity and hepatic mRNA expression in chicken embryos
    Caroline Egloff
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario, Canada K1N6N5
    Toxicol Lett 207:25-33. 2011
    ..None of the gene targets were responsive to BEHTBP exposure in CEH. The multi-tiered in vitro/in ovo screening approach was effective for assessing toxicological and molecular biological effects of these BFRs in an avian species...
  11. doi request reprint Induction of cytochrome P4501A by highly purified hexachlorobenzene in primary cultures of ring-necked pheasant and Japanese quail embryo hepatocytes
    Lukas J Mundy
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 155:498-505. 2012
    ....
  12. ncbi request reprint Tris(1,3-dichloro-2-propyl) phosphate perturbs the expression of genes involved in immune response and lipid and steroid metabolism in chicken embryos
    Amani Farhat
    Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5, Canada National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3, Canada
    Toxicol Appl Pharmacol 275:104-12. 2014
    ..Finally, our results are in concordance with the literature that describes TDCPP as a cancer-causing agent, since the majority of dysregulated genes were involved in cancer pathways...
  13. doi request reprint Cytochrome P4501A induction in primary cultures of embryonic European starling hepatocytes exposed to TCDD, PeCDF and TCDF
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics Department of Biology, University of Ottawa, Ottawa, ON, K1N 6N5, Canada
    Ecotoxicology 22:731-9. 2013
    ..However, starling hepatocytes are less sensitive than chicken hepatocytes to CYP1A4/5 induction by PeCDF and TCDF...
  14. doi request reprint In Ovo effects of two organophosphate flame retardants--TCPP and TDCPP--on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryos
    Amani Farhat
    Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Toxicol Sci 134:92-102. 2013
    ..The effects of TDCPP on the gallbladder indicate that the disturbance of lipid metabolism is a likely mechanism of toxicity. ..
  15. doi request reprint In vitro microarray analysis identifies genes in acute-phase response pathways that are down-regulated in the liver of chicken embryos exposed in ovo to PFUdA
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie, Ottawa, ON, Canada
    Toxicol In Vitro 27:1649-58. 2013
    ..These findings suggest in ovo exposure of birds to PFUdA may lead to post-hatch developmental deficiencies, such as impaired inflammatory response...
  16. doi request reprint Ethoxyresorufin O-deethylase induction by TCDD, PeCDF and TCDF in ring-necked pheasant and Japanese quail hepatocytes: Time-dependent effects on concentration-response curves
    Jessica C Hervé
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    Toxicol In Vitro 24:1301-5. 2010
    ....
  17. ncbi request reprint Use of an avian hepatocyte assay and the avian Toxchip Polymerse chain reaction array for testing prioritization of 16 organic flame retardants
    Emily Porter
    National Wildlife Research Centre, Environment Canada, Ottawa, Ontario, Canada
    Environ Toxicol Chem 33:573-82. 2014
    ....
  18. doi request reprint Technical-grade perfluorooctane sulfonate alters the expression of more transcripts in cultured chicken embryonic hepatocytes than linear perfluorooctane sulfonate
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Environ Toxicol Chem 30:2846-59. 2011
    ..These data show that T-PFOS elicits a greater transcriptional response in CEH than L-PFOS alone and demonstrates the importance of considering the isomer-specific toxicological properties of PFOS when assessing exposure risk...
  19. doi request reprint Sequence and in vitro function of chicken, ring-necked pheasant, and Japanese quail AHR1 predict in vivo sensitivity to dioxins
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    Environ Sci Technol 46:2967-75. 2012
    ..Our results support and expand previous in vitro and in vivo work that demonstrated ligand-dependent species differences in AHR1 affinity. The findings and methods will be of use for DLC risk assessments...
  20. pmc Effects of perfluoroalkyl compounds on mRNA expression levels of thyroid hormone-responsive genes in primary cultures of avian neuronal cells
    Viengtha Vongphachan
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    Toxicol Sci 120:392-402. 2011
    ..This is the first study to report that PFC exposure alters mRNA expression in primary cultures of avian neuronal cells and may provide insight into the possible mechanisms of action of PFCs in the avian brain...
  21. doi request reprint Highly purified hexachlorobenzene induces cytochrome P4501A in primary cultures of chicken embryo hepatocytes
    Lukas J Mundy
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Toxicol Appl Pharmacol 248:185-93. 2010
    ..The maximal EROD activity and CYP1A4/5 mRNA expression differed greatly between HCB and TCDD, and may contribute to an overestimation of the ReP value calculated for highly purified HCB...
  22. doi request reprint Pipping success and liver mRNA expression in chicken embryos exposed in ovo to C8 and C11 perfluorinated carboxylic acids and C10 perfluorinated sulfonate
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario K1N 6N5, Canada
    Toxicol Lett 190:134-9. 2009
    ..Based on the present results for white leghorn chickens, current environmental concentrations of PFOA, PFUdA and PFDS are unlikely to affect the hatching success of wild birds...
  23. doi request reprint Transcriptional profiles in the cerebral hemisphere of chicken embryos following in ovo perfluorohexane sulfonate exposure
    Cristina G Cassone
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 129:380-91. 2012
    ....
  24. doi request reprint Species-specific relative AHR1 binding affinities of 2,3,4,7,8-pentachlorodibenzofuran explain avian species differences in its relative potency
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada Environment Canada, National Wildlife Research Centre, Ottawa, Ontario K1A 0H3, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 161:21-5. 2014
    ..The current report introduces a COS-7 whole-cell binding assay and provides a mechanistic explanation for differential relative potencies of PeCDF among species of birds. ..
  25. doi request reprint Cytochrome P4501A induction in avian hepatocyte cultures exposed to polychlorinated biphenyls: comparisons with AHR1-mediated reporter gene activity and in ovo toxicity
    Gillian E Manning
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    Toxicol Appl Pharmacol 266:38-47. 2013
    ..Relative potency values were generally similar between the LRG and EROD assays and indicate that the relative potency of some PCBs may differ among species...
  26. ncbi request reprint Fluorescent RNA arbitrarily primed polymerase chain reaction. A new differential display approach to detect contaminant-induced alterations of gene expression in wildlife species
    Doug Crump
    National Wildlife Research Center, Canadian Wildlife Service, Carleton University, Ottawa, Ontario, Canada
    Methods Mol Biol 410:15-27. 2008
    ..g., cultured cells, tissue). This straightforward, safe, and cost-effective approach represents a useful alternative to the radiometric-based RAP-PCR method...
  27. doi request reprint Effects of 18 perfluoroalkyl compounds on mRNA expression in chicken embryo hepatocyte cultures
    Nathan J Hickey
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario K1A 0H3, Canada
    Toxicol Sci 111:311-20. 2009
    ..This study is the first to expose CEH cultures to multiple PFCs in vitro and demonstrates that exposure to PFC solutions of different isomeric content or chain length causes variable transcriptional responses...
  28. doi request reprint Cytochrome P4501A induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin and two chlorinated dibenzofurans in primary hepatocyte cultures of three avian species
    Jessica C Hervé
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ontario, Canada
    Toxicol Sci 113:380-91. 2010
    ..This study indicates that PeCDF is more potent than TCDD in ring-necked pheasant and Japanese quail hepatocytes and that ring-necked pheasant, Japanese quail, and chicken hepatocytes are equally sensitive to CYP1A induction by PeCDF...
  29. doi request reprint A luciferase reporter gene assay and aryl hydrocarbon receptor 1 genotype predict the LD50 of polychlorinated biphenyls in avian species
    Gillian E Manning
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    Toxicol Appl Pharmacol 263:390-401. 2012
    ....
  30. ncbi request reprint Expression profiles of novel thyroid hormone-responsive genes and proteins in the tail of Xenopus laevis tadpoles undergoing precocious metamorphosis
    Caren C Helbing
    Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada V8W 3P6
    Mol Endocrinol 17:1395-409. 2003
    ....
  31. pmc Exposure to the herbicide acetochlor alters thyroid hormone-dependent gene expression and metamorphosis in Xenopus Laevis
    Doug Crump
    Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
    Environ Health Perspect 110:1199-205. 2002
    ..The morphologic changes observed are preceded by alterations in gene expression profiles detected in the tadpole tail, and the nature of these profiles suggest a novel mechanism of action for acetochlor...
  32. ncbi request reprint Distinctive gene profiles occur at key points during natural metamorphosis in the Xenopus laevis tadpole tail
    Nik Veldhoen
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
    Dev Dyn 225:457-68. 2002
    ..This work establishes an important baseline for determining the mechanisms whereby tissues undergo differing metamorphic fates...