Wei Gang Hu

Summary

Affiliation: Defence Research and Development Canada
Country: Canada

Publications

  1. ncbi request reprint Infectivity variation and genetic diversity among strains of Western equine encephalitis virus
    Les P Nagata
    Chemical and Biological Defence Section, Defence Research and Development Canada Suffield, Station Main, Medicine Hat, AB T1A 8K6, Canada
    J Gen Virol 87:2353-61. 2006
  2. ncbi request reprint Functional enhancement of a partially active single-chain variable fragment antibody to Venezuelan equine encephalitis virus
    Azhar Z Alvi
    AFAM ALVI Researchers and Consultants, Medicine Hat, Canada
    Viral Immunol 16:213-22. 2003
  3. ncbi request reprint Development of immunofiltration assay by light addressable potentiometric sensor with genetically biotinylated recombinant antibody for rapid identification of Venezuelan equine encephalitis virus
    Wei Gang Hu
    Chemical and Biological Defence Section, Defence R and D Canada Suffield, Medicine Hat, Alberta, Canada
    J Immunol Methods 289:27-35. 2004
  4. ncbi request reprint Cloning and expression in E. coli of a functional Fab fragment obtained from single human lymphocyte against anthrax toxin
    Saad A Masri
    Canadian Food Inspection Agency, Centre for Plant Health, 8801, East Saanich Road, Sidney, British Columbia, Canada
    Mol Immunol 44:2101-6. 2007
  5. ncbi request reprint Complete protection of mice against a lethal dose challenge of western equine encephalitis virus after immunization with an adenovirus-vectored vaccine
    Josh Q H Wu
    Chemical and Biological Defence Section, Defence Research and Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada
    Vaccine 25:4368-75. 2007
  6. pmc Conformation-dependent high-affinity potent ricin-neutralizing monoclonal antibodies
    Wei Gang Hu
    Defence Research and Development Canada Suffield, P O Box 4000, Station Main, Medicine Hat, AB, Canada T1A 8K6
    Biomed Res Int 2013:471346. 2013
  7. pmc Humanization and characterization of an anti-ricin neutralization monoclonal antibody
    Wei Gang Hu
    Defence Research and Development Canada Suffield, Medicine Hat, Alberta, Canada
    PLoS ONE 7:e45595. 2012
  8. ncbi request reprint Antibody gene-based prophylaxis and therapy for biodefence
    Wei Gang Hu
    Defense Research and Development Canada Suffield, Alberta, Canada
    Hum Vaccin 4:74-8. 2008
  9. doi request reprint A novel approach to development of monoclonal antibodies using native antigen for immunization and recombinant antigen for screening
    Wei Gang Hu
    Defence Research and Development Canada Suffield, Medicine Hat, Alberta, Canada
    Hybridoma (Larchmt) 27:307-11. 2008
  10. ncbi request reprint Humanization and mammalian expression of a murine monoclonal antibody against Venezuelan equine encephalitis virus
    Wei Gang Hu
    Defence Research Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada T1A 8K6
    Vaccine 25:3210-4. 2007

Collaborators

Detail Information

Publications21

  1. ncbi request reprint Infectivity variation and genetic diversity among strains of Western equine encephalitis virus
    Les P Nagata
    Chemical and Biological Defence Section, Defence Research and Development Canada Suffield, Station Main, Medicine Hat, AB T1A 8K6, Canada
    J Gen Virol 87:2353-61. 2006
    ..Furthermore, potential amino acid differences in some positions between the two groups were identified, suggesting that these amino acid variations contributed to the observed differences in virulence...
  2. ncbi request reprint Functional enhancement of a partially active single-chain variable fragment antibody to Venezuelan equine encephalitis virus
    Azhar Z Alvi
    AFAM ALVI Researchers and Consultants, Medicine Hat, Canada
    Viral Immunol 16:213-22. 2003
    ..Sequence analysis revealed that only MA116-15 had incorporated the three intended base insertions. The varying degrees of reactivity of MA116 clones are discussed in light of their molecular changes...
  3. ncbi request reprint Development of immunofiltration assay by light addressable potentiometric sensor with genetically biotinylated recombinant antibody for rapid identification of Venezuelan equine encephalitis virus
    Wei Gang Hu
    Chemical and Biological Defence Section, Defence R and D Canada Suffield, Medicine Hat, Alberta, Canada
    J Immunol Methods 289:27-35. 2004
    ..Furthermore, the potential for batch-to-batch variability resulting from inequality in the number of biotin molecules labeled per antibody molecule is eliminated...
  4. ncbi request reprint Cloning and expression in E. coli of a functional Fab fragment obtained from single human lymphocyte against anthrax toxin
    Saad A Masri
    Canadian Food Inspection Agency, Centre for Plant Health, 8801, East Saanich Road, Sidney, British Columbia, Canada
    Mol Immunol 44:2101-6. 2007
    ..This approach allowed us to obtain human Fab that retained the natural heavy and light chain pairing, which is supposed to have a high antigen-binding affinity...
  5. ncbi request reprint Complete protection of mice against a lethal dose challenge of western equine encephalitis virus after immunization with an adenovirus-vectored vaccine
    Josh Q H Wu
    Chemical and Biological Defence Section, Defence Research and Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada
    Vaccine 25:4368-75. 2007
    ..In addition, we showed that passive transfer of serum from the Ad5-WEEV-immunized mice could partially control WEEV infection. These results demonstrate that HAd5 vectors are promising for WEEV vaccine development...
  6. pmc Conformation-dependent high-affinity potent ricin-neutralizing monoclonal antibodies
    Wei Gang Hu
    Defence Research and Development Canada Suffield, P O Box 4000, Station Main, Medicine Hat, AB, Canada T1A 8K6
    Biomed Res Int 2013:471346. 2013
    ....
  7. pmc Humanization and characterization of an anti-ricin neutralization monoclonal antibody
    Wei Gang Hu
    Defence Research and Development Canada Suffield, Medicine Hat, Alberta, Canada
    PLoS ONE 7:e45595. 2012
    ..In addition, administered 6 hours post-challenge, hD9 could still rescue 50% of the mice. The hD9 has the potential to be used for prophylactic or therapeutic purposes against ricin poisoning...
  8. ncbi request reprint Antibody gene-based prophylaxis and therapy for biodefence
    Wei Gang Hu
    Defense Research and Development Canada Suffield, Alberta, Canada
    Hum Vaccin 4:74-8. 2008
    ....
  9. doi request reprint A novel approach to development of monoclonal antibodies using native antigen for immunization and recombinant antigen for screening
    Wei Gang Hu
    Defence Research and Development Canada Suffield, Medicine Hat, Alberta, Canada
    Hybridoma (Larchmt) 27:307-11. 2008
    ..This novel approach to the development of MAbs should be applicable to the production of MAbs to other microbes, especially to those from which antigens can hardly be purified to a high degree...
  10. ncbi request reprint Humanization and mammalian expression of a murine monoclonal antibody against Venezuelan equine encephalitis virus
    Wei Gang Hu
    Defence Research Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada T1A 8K6
    Vaccine 25:3210-4. 2007
    ..After expression in HEK 293 cells and purification by protein L column, the humanized antibody was demonstrated to retain antigen-binding specificity and neutralizing activity...
  11. doi request reprint Generation of a recombinant full-length human antibody binding to botulinum neurotoxin A
    Wei Gang Hu
    Defence Research and Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada T1A8K6
    Appl Biochem Biotechnol 160:1206-16. 2010
    ....
  12. ncbi request reprint Cloning, expression and purification of envelope proteins E1 and E2 of western equine encephalitis virus and potential use of them as antigens in immunoassays
    Wei Gang Hu
    Biotechnology Section, Defence Research and Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada T1A 8K6
    Vet Microbiol 128:374-9. 2008
    ....
  13. doi request reprint A recombinant humanized monoclonal antibody completely protects mice against lethal challenge with Venezuelan equine encephalitis virus
    Wei Gang Hu
    Defence Research and Development Canada Suffield, Medicine Hat, Alberta, Canada T1A 8K6
    Vaccine 28:5558-64. 2010
    ....
  14. ncbi request reprint Efficacy of DNA vaccination against western equine encephalitis virus infection
    Les P Nagata
    Chemical Biological Defence Section, Defence R and D Canada Suffield, Box 4000, Station Main, Medicine Hat, Alta, Canada T1A 8K6
    Vaccine 23:2280-3. 2005
    ..The efficacy results suggest a DNA vaccine may be a promising approach against WEE infection...
  15. ncbi request reprint Development and characterization of a novel fusion protein composed of a human IgG1 heavy chain constant region and a single-chain fragment variable antibody against Venezuelan equine encephalitis virus
    Wei Gang Hu
    Chemical Biological Defence Section, Defence Research and Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada T1A 8K6
    J Biochem 133:59-66. 2003
    ..This work forms the basis for characterization of the recombinant antibody as to efficacy in vivo...
  16. ncbi request reprint Recombinant anti-botulinum neurotoxin A single-chain variable fragment antibody generated using a phage display system
    David C W Mah
    Canada West Biosciences, Calgary, Alberta, Canada
    Hybrid Hybridomics 22:277-83. 2003
    ..Forty-eight clones were found to be BoNT/A-reactive. The most reactive clone, designated D12, was selected for further study. The scFv gene of D12 was subcloned into a Pichia pastoris vector, and expression in yeast was evaluated...
  17. ncbi request reprint Genetic engineering of streptavidin-binding peptide tagged single-chain variable fragment antibody to Venezuelan equine encephalitis virus
    Wei Gang Hu
    Chemical Biological Defence Section, Defence Research and Development Canada Suffield, Box 4000, Station Main, Medicine Hat, Alberta, Canada T1A 8K6
    Hybrid Hybridomics 21:415-20. 2002
    ....
  18. doi request reprint Microbead electrochemiluminescence immunoassay for detection and identification of Venezuelan equine encephalitis virus
    Xiaojiang Dai
    Defence Research and Development Canada Suffield, PO Box 4000, Station Main, Medicine Hat, AB, Canada
    J Virol Methods 169:274-81. 2010
    ..Results have demonstrated a sensitive, rapid, and fully automated ECL immunoassay for detection and identification of VEEV...
  19. ncbi request reprint Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane
    Daxin Peng
    Vaccine Research Section, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD, USA
    FEBS J 274:5350-9. 2007
    ..Both the lipid A and linker moieties contribute to the LOS toxicity...
  20. ncbi request reprint Expression of furin-linked Fab fragments against anthrax toxin in a single mammalian expression vector
    Christopher D Reed
    Canadian Food Inspection Agency, Centre for Plant Health, 8801 East Saanich Road, Sidney, BC, Canada V8L 1H3
    Protein Expr Purif 54:261-6. 2007
    ..The human Fab antibodies produced by this technique were functional when tested in Western blots using the recombinant PA antigen as the target...
  21. ncbi request reprint Exploration of Moraxella catarrhalis outer membrane proteins, CD and UspA, as new carriers for lipooligosaccharide-based conjugates
    Wei Gang Hu
    Vaccine Research Section, National Institute on Deafness and Other Communication Disorders, Rockville, MD 20850, USA
    FEMS Immunol Med Microbiol 41:109-15. 2004
    ....