Nikhil A Thomas

Summary

Affiliation: Dalhousie University
Country: Canada

Publications

  1. ncbi request reprint The bacterial virulence factor NleA inhibits cellular protein secretion by disrupting mammalian COPII function
    Jinoh Kim
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell Host Microbe 2:160-71. 2007
  2. pmc Expanded roles for multicargo and class 1B effector chaperones in type III secretion
    Nikhil A Thomas
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada
    J Bacteriol 194:3767-73. 2012
  3. ncbi request reprint Hierarchical delivery of an essential host colonization factor in enteropathogenic Escherichia coli
    Nikhil A Thomas
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
    J Biol Chem 282:29634-45. 2007
  4. ncbi request reprint CesT is a multi-effector chaperone and recruitment factor required for the efficient type III secretion of both LEE- and non-LEE-encoded effectors of enteropathogenic Escherichia coli
    Nikhil A Thomas
    Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada
    Mol Microbiol 57:1762-79. 2005
  5. pmc A novel C-terminal region within the multicargo type III secretion chaperone CesT contributes to effector secretion
    Thangadurai Ramu
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada
    J Bacteriol 195:740-56. 2013
  6. pmc A comprehensive proteomic analysis of the type III secretome of Citrobacter rodentium
    Wanyin Deng
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    J Biol Chem 285:6790-800. 2010
  7. pmc Role of EscU auto-cleavage in promoting type III effector translocation into host cells by enteropathogenic Escherichia coli
    Jenny Lee Thomassin
    Department of Microbiology and Immunology, Dalhousie University, 5850 College Street, PO Box 15000, Halifax, Nova Scotia, B3H 4R2 Canada
    BMC Microbiol 11:205. 2011
  8. ncbi request reprint Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157:H7
    Samantha Gruenheid
    Biotechnology Laboratory, University of British Columbia, Room 237, Wesbrook Building, 6174 University Boulevard, Vancouver, BC, V6T 1Z3, Canada
    Mol Microbiol 51:1233-49. 2004
  9. ncbi request reprint Structural characterization of the molecular platform for type III secretion system assembly
    Calvin K Yip
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3
    Nature 435:702-7. 2005
  10. doi request reprint Characterization of the type III secretion associated low calcium response genes of Vibrio parahaemolyticus RIMD2210633
    Darren Sarty
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada
    Can J Microbiol 58:1306-15. 2012

Collaborators

  • B B Finlay
  • Jan K Rainey
  • Xiang He
  • Roger O Ebanks
  • Lori L Graham
  • Leonard J Foster
  • Tony Pawson
  • Keith Ashman
  • Philip R Hardwidge
  • Wanyin Deng
  • Jenny Lee Thomassin
  • Thangadurai Ramu
  • Elizabeth A Frey
  • Samantha Gruenheid
  • Erin Brouwers
  • Darren Sarty
  • Jose L Puente
  • Víctor A García-Angulo
  • Yuling Li
  • Jinoh Kim
  • Pavel Metalnikov
  • Calvin K Yip
  • Jason Leblanc
  • Madhulika Esther Prasad
  • Erica Connors
  • Amit Mishra
  • Cheryl Rafuse
  • Noel T Baker
  • Irene Ma
  • Euan L Thomson
  • Hong B Yu
  • Carmen L de Hoog
  • Matthew A Croxen
  • Keyrillos Rizg
  • Tessa Chaworth-Musters
  • Randy Schekman
  • Paula I Lario
  • Sau Fung Lee
  • Natalie C Strynadka
  • Ajitha Thanabalasuriar
  • J Chris Fromme
  • Elizabeth L Hartland
  • Samuel I Miller
  • Heather B Felise
  • Richard A Pfuetzner
  • Natalie C J Strynadka
  • Tyler G Kimbrough
  • Marija Vuckovic
  • Inna Sekirov
  • Paul O'Donnell
  • David Goode
  • Nathaniel F Brown

Detail Information

Publications14

  1. ncbi request reprint The bacterial virulence factor NleA inhibits cellular protein secretion by disrupting mammalian COPII function
    Jinoh Kim
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell Host Microbe 2:160-71. 2007
    ..Collectively, these findings indicate that disruption of COPII function in host cells contributes to the virulence of EPEC and EHEC...
  2. pmc Expanded roles for multicargo and class 1B effector chaperones in type III secretion
    Nikhil A Thomas
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada
    J Bacteriol 194:3767-73. 2012
    ..The evidence implicates multicargo and class 1B T3SC in effector binding and stabilization, in addition to T3SS recruitment and docking events...
  3. ncbi request reprint Hierarchical delivery of an essential host colonization factor in enteropathogenic Escherichia coli
    Nikhil A Thomas
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
    J Biol Chem 282:29634-45. 2007
    ..Collectively, the results suggest a coordinated mechanism involving both Tir and CesT for type III effector injection into host cells...
  4. ncbi request reprint CesT is a multi-effector chaperone and recruitment factor required for the efficient type III secretion of both LEE- and non-LEE-encoded effectors of enteropathogenic Escherichia coli
    Nikhil A Thomas
    Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada
    Mol Microbiol 57:1762-79. 2005
    ..These findings indicate that CesT is centrally involved in recruiting multiple type III effectors to the T3SS via EscN for efficient secretion, and functionally redefine the role of CesT in multiple type III effector interactions...
  5. pmc A novel C-terminal region within the multicargo type III secretion chaperone CesT contributes to effector secretion
    Thangadurai Ramu
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada
    J Bacteriol 195:740-56. 2013
    ..The findings implicate the CesT C terminus in effector secretion and contribute to a model for multiple-cargo chaperone function and effector translocation into host cells during infection...
  6. pmc A comprehensive proteomic analysis of the type III secretome of Citrobacter rodentium
    Wanyin Deng
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
    J Biol Chem 285:6790-800. 2010
    ....
  7. pmc Role of EscU auto-cleavage in promoting type III effector translocation into host cells by enteropathogenic Escherichia coli
    Jenny Lee Thomassin
    Department of Microbiology and Immunology, Dalhousie University, 5850 College Street, PO Box 15000, Halifax, Nova Scotia, B3H 4R2 Canada
    BMC Microbiol 11:205. 2011
    ..The crystal structure of the C-terminal portion of EscU from enteropathogenic Escherichia coli (EPEC) suggests this auto-cleaving protein provides an interface for substrate interactions involved in type III secretion events...
  8. ncbi request reprint Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157:H7
    Samantha Gruenheid
    Biotechnology Laboratory, University of British Columbia, Room 237, Wesbrook Building, 6174 University Boulevard, Vancouver, BC, V6T 1Z3, Canada
    Mol Microbiol 51:1233-49. 2004
    ..coli and Citrobacter rodentium, and was absent from non-pathogenic strains of E. coli and non-LEE-containing pathogens. NleA was determined to play a key role in virulence of C. rodentium in a mouse infection model...
  9. ncbi request reprint Structural characterization of the molecular platform for type III secretion system assembly
    Calvin K Yip
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3
    Nature 435:702-7. 2005
    ..We propose that the YscJ/PrgK protein family functions as an essential molecular platform for TTSS assembly...
  10. doi request reprint Characterization of the type III secretion associated low calcium response genes of Vibrio parahaemolyticus RIMD2210633
    Darren Sarty
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada
    Can J Microbiol 58:1306-15. 2012
    ..parahaemolyticus-mediated host-cell cytotoxicity during infection. These approaches should be valuable towards future detailed genetic and biochemical analyses of T3SS-1 in V. parahaemolyticus...
  11. doi request reprint Dual temporal transcription activation mechanisms control cesT expression in enteropathogenic Escherichia coli
    Erin Brouwers
    Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 4R2, Canada
    Microbiology 158:2246-61. 2012
    ..We suggest that A/E pathogenic E. coli have evolved a mechanism to ready the cell for CesT protein expression in support of infection prior to Ler- and GrlA-related activities...
  12. ncbi request reprint Establishing order for type III secretion substrates--a hierarchical process
    Nikhil A Thomas
    Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada
    Trends Microbiol 11:398-403. 2003
  13. pmc Regulation of expression and secretion of NleH, a new non-locus of enterocyte effacement-encoded effector in Citrobacter rodentium
    Víctor A García-Angulo
    Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad 2001, Cuernavaca, Morelos 62210, Mexico
    J Bacteriol 190:2388-99. 2008
    ..A C. rodentium nleH mutant shows a moderate defect during the colonization of C57BL/6 mice at early stages of infection...
  14. ncbi request reprint Mutants in flaI and flaJ of the archaeon Methanococcus voltae are deficient in flagellum assembly
    Nikhil A Thomas
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Mol Microbiol 46:879-87. 2002
    ..These findings further support a model describing the archaeal flagellum as a novel prokaryotic motility structure...