Christopher Leonard Brett
Affiliation: Concordia University
McNally E, Brett C. The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation. Nat Commun. 2018;9:5358 pubmed publisher
..How this ILF pathway compares to the MVB pathway and potentially contributes to physiology is discussed. ..
Karim M, Samyn D, Brett C. A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion. Methods Mol Biol. 2019;1860:289-301 pubmed publisher
..This method accommodates a multitude of approaches (e.g., genetics, addition of purified protein reagents) to study this process in isolation, and in theory could be repurposed to study other SNARE-mediated fusion events within cells. ..
Mattie S, Kazmirchuk T, Mui J, Vali H, Brett C. Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy. Methods Mol Biol. 2019;1860:361-377 pubmed publisher
Karim M, McNally E, Samyn D, Mattie S, Brett C. Rab-Effector-Kinase Interplay Modulates Intralumenal Fragment Formation during Vacuole Fusion. Dev Cell. 2018;47:80-97.e6 pubmed publisher
..We propose that HOPS coordinates Rab, kinase, and SNARE cycles to modulate ILF size during vacuole fusion, regulating lipid and protein turnover important for quality control and membrane integrity. ..
Mattie S, McNally E, Karim M, Vali H, Brett C. How and why intralumenal membrane fragments form during vacuolar lysosome fusion. Mol Biol Cell. 2017;28:309-321 pubmed publisher
McNally E, Karim M, Brett C. Selective Lysosomal Transporter Degradation by Organelle Membrane Fusion. Dev Cell. 2017;40:151-167 pubmed publisher
Karim M, Brett C. The Na+(K+)/H+ exchanger Nhx1 controls multivesicular body-vacuolar lysosome fusion. Mol Biol Cell. 2018;29:317-325 pubmed publisher
..All contributing mechanisms are evolutionarily conserved offering new insight into the etiology of human disorders linked to loss of endosomal NHE function. ..