N G Seidah

Summary

Affiliation: Clinical Research Institute of Montreal
Country: Canada

Publications

  1. ncbi request reprint The biology and therapeutic targeting of the proprotein convertases
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal affiliated to University of Montreal, 110 Pine Ave West, Montreal, Quebec H2W 1R7, Canada
    Nat Rev Drug Discov 11:367-83. 2012
  2. pmc Proprotein convertase subtilisin/kexin type 9 (PCSK9) can mediate degradation of the low density lipoprotein receptor-related protein 1 (LRP-1)
    Maryssa Canuel
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Quebec, Canada
    PLoS ONE 8:e64145. 2013
  3. pmc PCSK9 prosegment chimera as novel inhibitors of LDLR degradation
    Yascara Grisel Luna Saavedra
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Affiliated to the Université de Montréal, Montreal, Quebec, Canada
    PLoS ONE 8:e72113. 2013
  4. pmc A novel mouse model of Alzheimer's disease with chronic estrogen deficiency leads to glial cell activation and hypertrophy
    Annik Prat
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, H2W 1R7, Canada
    J Aging Res 2011:251517. 2011
  5. pmc The multifaceted proprotein convertases: their unique, redundant, complementary, and opposite functions
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal IRCM, affiliated with the University of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 288:21473-81. 2013
  6. ncbi request reprint Proprotein convertase subtilisin kexin 9 (PCSK9) inhibitors in the treatment of hypercholesterolemia and other pathologies
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal IRCM, affiliated with the University of Montreal, 110 Pine Avenue West Montreal, Québec H2W1R7, Canada
    Curr Pharm Des 19:3161-72. 2013
  7. pmc Chloroquine is a potent inhibitor of SARS coronavirus infection and spread
    Martin J Vincent
    Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Virol J 2:69. 2005
  8. pmc The proprotein convertase PC5/6 is protective against intestinal tumorigenesis: in vivo mouse model
    Xiaowei Sun
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Quebec, Canada
    Mol Cancer 8:73. 2009
  9. pmc The secretory proprotein convertase neural apoptosis-regulated convertase 1 (NARC-1): liver regeneration and neuronal differentiation
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, H2W 1R7 Canada
    Proc Natl Acad Sci U S A 100:928-33. 2003
  10. ncbi request reprint Precursor convertases in the secretory pathway, cytosol and extracellular milieu
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, Canada H2W 1R7
    Essays Biochem 38:79-94. 2002

Research Grants

Detail Information

Publications142 found, 100 shown here

  1. ncbi request reprint The biology and therapeutic targeting of the proprotein convertases
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal affiliated to University of Montreal, 110 Pine Ave West, Montreal, Quebec H2W 1R7, Canada
    Nat Rev Drug Discov 11:367-83. 2012
    ....
  2. pmc Proprotein convertase subtilisin/kexin type 9 (PCSK9) can mediate degradation of the low density lipoprotein receptor-related protein 1 (LRP-1)
    Maryssa Canuel
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Quebec, Canada
    PLoS ONE 8:e64145. 2013
    ..Identification of PCSK9 targets should allow a better understanding of the consequences of PCSK9 inhibition for lowering LDLc and tumor metastasis...
  3. pmc PCSK9 prosegment chimera as novel inhibitors of LDLR degradation
    Yascara Grisel Luna Saavedra
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Affiliated to the Université de Montréal, Montreal, Quebec, Canada
    PLoS ONE 8:e72113. 2013
    ..Our data suggest a novel strategic approach for the design and isolation of PCSK9 inhibitors. ..
  4. pmc A novel mouse model of Alzheimer's disease with chronic estrogen deficiency leads to glial cell activation and hypertrophy
    Annik Prat
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, H2W 1R7, Canada
    J Aging Res 2011:251517. 2011
    ....
  5. pmc The multifaceted proprotein convertases: their unique, redundant, complementary, and opposite functions
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal IRCM, affiliated with the University of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 288:21473-81. 2013
    ..In vivo, PCs exhibit unique and often essential functions during development and/or in adulthood, but certain convertases also exhibit complementary, redundant, or opposite functions. ..
  6. ncbi request reprint Proprotein convertase subtilisin kexin 9 (PCSK9) inhibitors in the treatment of hypercholesterolemia and other pathologies
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal IRCM, affiliated with the University of Montreal, 110 Pine Avenue West Montreal, Québec H2W1R7, Canada
    Curr Pharm Des 19:3161-72. 2013
    ..This opens the door to novel applications of PCSK9 inhibitors/silencers in cancer/metastasis...
  7. pmc Chloroquine is a potent inhibitor of SARS coronavirus infection and spread
    Martin J Vincent
    Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Virol J 2:69. 2005
    ..Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available...
  8. pmc The proprotein convertase PC5/6 is protective against intestinal tumorigenesis: in vivo mouse model
    Xiaowei Sun
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Quebec, Canada
    Mol Cancer 8:73. 2009
    ..Aside from the role of furin in salivary gland tumorigenesis, no other in vivo genetic model of PC-knockout was reported in relation to cancer development...
  9. pmc The secretory proprotein convertase neural apoptosis-regulated convertase 1 (NARC-1): liver regeneration and neuronal differentiation
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, H2W 1R7 Canada
    Proc Natl Acad Sci U S A 100:928-33. 2003
    ..5 telencephalon cells led to enhanced recruitment of undifferentiated neural progenitor cells into the neuronal lineage, suggesting that NARC-1 is implicated in the differentiation of cortical neurons...
  10. ncbi request reprint Precursor convertases in the secretory pathway, cytosol and extracellular milieu
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, Canada H2W 1R7
    Essays Biochem 38:79-94. 2002
    ..NRDc could represent the equivalent of the PCs in the cytosol or the extracellular space...
  11. doi request reprint What lies ahead for the proprotein convertases?
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Canada
    Ann N Y Acad Sci 1220:149-61. 2011
    ..In vivo studies demonstrated that PCs play major roles in health and disease states...
  12. ncbi request reprint PCSK9 as a therapeutic target of dyslipidemia
    Nabil G Seidah
    Clinical Research Institute of Montreal, Laboratory of Biochemical Neuroendocrinology, 110 Pine Ave West, Montreal, QC, H2W 1R7 Canada
    Expert Opin Ther Targets 13:19-28. 2009
    ..Its gene is associated with the development of familial hypercholesterolemia. mRNA silencing or inhibition of PCSK9-induced degradation of LDLR may be used to treat this disease...
  13. doi request reprint The activation and physiological functions of the proprotein convertases
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada
    Int J Biochem Cell Biol 40:1111-25. 2008
    ..These data provide evidence that an inhibitor of PCSK9-LDLR interaction is a viable target for the development of a novel cholesterol lowering drug in conjunction with the classical statins...
  14. ncbi request reprint Proprotein and prohormone convertases: a family of subtilases generating diverse bioactive polypeptides
    N G Seidah
    Laboratories of Biochemical and Molecular Neuroendocrinology and the Protein Engineering Network of Centres of Excellence, Clinical Research Institute of Montreal, Canada
    Brain Res 848:45-62. 1999
    ....
  15. ncbi request reprint The proprotein convertases are potential targets in the treatment of dyslipidemia
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal, Quebec, H2W 1R7, Canada
    J Mol Med (Berl) 85:685-96. 2007
    ..Specific inhibitors/modulators of the other PCs should find novel therapeutic applications in the control of PC-regulated pathologies...
  16. pmc Unexpected similarity between the cytosolic West Nile virus NS3 and the secretory furin-like serine proteinases
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal, QC, H2W 1R7, Canada
    Biochem J 393:e1-3. 2006
    ..Structural modelling based on Dengue virus NS3 provided a possible rationale for the observed cleavage specificity of West Nile virus NS3...
  17. ncbi request reprint Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, Furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2
    N G Seidah
    J A DeSève Laboratories of Biochemical, Clinical Research Institute of Montreal, Quebec, Canada
    Mol Endocrinol 5:111-22. 1991
    ..The genes coding for mPC1 and mPC2 map to the murine chromosomes 13 (band 13c) and 2 (2F3-2H2 region), respectively...
  18. ncbi request reprint cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases
    N G Seidah
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    DNA Cell Biol 9:415-24. 1990
    ..8 kb) demonstrated tissue and cellular specificity of expression, only within endocrine and neuroendocrine cells. These data suggest that mPC1 and mPC2 represent prime candidates for tissue-specific pro-hormone converting proteinases...
  19. ncbi request reprint The prosegments of furin and PC7 as potent inhibitors of proprotein convertases. In vitro and ex vivo assessment of their efficacy and selectivity
    M Zhong
    Laboratory of Biochemical, Clinical Research Institute of Montreal, University of Montreal, Montreal, Quebec H2W 1R7
    J Biol Chem 274:33913-20. 1999
    ..Thus, we have demonstrated for the first time that PC prosegments, expressed ex vivo as independent domains, can act in trans to inhibit precursor maturation by intracellular PCs...
  20. pmc Comparative biosynthesis, covalent post-translational modifications and efficiency of prosegment cleavage of the prohormone convertases PC1 and PC2: glycosylation, sulphation and identification of the intracellular site of prosegment cleavage of PC1 and P
    S Benjannet
    J A DeSève Laboratory of Molecular Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    Biochem J 294:735-43. 1993
    ..In order to test the possibility that zymogen processing is performed by furin, we co-expressed this convertase with either pro-PC1 or pro-PC2. The data demonstrated the inability of furin to cleave either proenzyme...
  21. pmc Mammalian subtilisin/kexin isozyme SKI-1: A widely expressed proprotein convertase with a unique cleavage specificity and cellular localization
    N G Seidah
    Laboratory of Biochemical, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, Canada H2W 1R7
    Proc Natl Acad Sci U S A 96:1321-6. 1999
    ..In vitro studies suggest that SKI-1 is a Ca2+-dependent serine proteinase exhibiting a wide pH optimum for cleavage of pro-brain-derived neurotrophic factor...
  22. pmc Cellular processing of the nerve growth factor precursor by the mammalian pro-protein convertases
    N G Seidah
    J A DeSève Laboratories of Biochemical and Molecular Neuroendocrinology, Clinical Research Institute of Montreal, University of Montreal, Canada
    Biochem J 314:951-60. 1996
    ..This comparative study shows that the three candidate mammalian subtilisin/kexin-like convertases identified process proNGF into NGF and that the nature of the final processed products is dependent on the intracellular environment...
  23. pmc The isoforms of proprotein convertase PC5 are sorted to different subcellular compartments
    I De Bie
    J A De Sève Laboraotry of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, QC, Canada
    J Cell Biol 135:1261-75. 1996
    ..Thus, a single PC5 gene generates COOH-terminally modified isoforms with different sorting signals directing these proteins to distinct subcellular localization, thereby allowing them to process their appropriate substrates...
  24. ncbi request reprint Mouse plasma kallikrein: cDNA structure, enzyme characterization, and comparison of protein and mRNA levels among species
    N G Seidah
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    DNA Cell Biol 9:737-48. 1990
    ..The regulation of the mRNA stability and/or turnover rate of PK may possibly be affected by its 5' end in a species-dependent manner...
  25. ncbi request reprint The family of subtilisin/kexin like pro-protein and pro-hormone convertases: divergent or shared functions
    N G Seidah
    JA DeSève Laboratories of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Que, Canada
    Biochimie 76:197-209. 1994
    ..Although the genetic organization of the convertase genes is very similar, they exhibit unique promoter sequences and only furin and PACE4 genes are localized on the same chromosome...
  26. pmc Enzymic characterization in vitro of recombinant proprotein convertase PC4
    A Basak
    Molecular Neuroendocrinology Laboratory, Clinical Research Institute of Montreal, Montreal, Que, Canada
    Biochem J 343:29-37. 1999
    ..PC4A was also inhibited by transition-metal ions (Cu(2+)>Hg(2+)>Zn(2+) Ni(2+)>Co(2+)), as well as by small peptide semicarbazones (SCs), such as Arg-Lys-Lys-Arg-SC (K(i) 0.75 microM) and Arg-Ser-Lys-Arg-SC (K(i) 11.4 microM)...
  27. pmc cDNA structure of the mouse and rat subtilisin/kexin-like PC5: a candidate proprotein convertase expressed in endocrine and nonendocrine cells
    J Lusson
    J A DeSève Laboratorie of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, PQ, Canada
    Proc Natl Acad Sci U S A 90:6691-5. 1993
    ..In situ hybridization of rat brain sections demonstrated a unique distribution of PC5 compared to PC1, PC2, and furin...
  28. ncbi request reprint Structure-function studies on the biosynthesis and bioactivity of the precursor convertase PC2 and the formation of the PC2/7B2 complex
    S Benjannet
    J A de Sève Laboratory of Molecular Neuroendocrinology, Clinical Research Institute of Montreal, Canada
    FEBS Lett 362:151-5. 1995
    ....
  29. ncbi request reprint Structure of the gene for the testis-specific proprotein convertase 4 and of its alternate messenger RNA isoforms
    M Mbikay
    Laboratoires de Neuroendocrinologie, Universite de Montreal, Quebec, Canada
    Genomics 20:231-7. 1994
    ..The multiple forms of PC4 mRNA can all be explained by alternate splicing of primary transcripts of a single gene...
  30. ncbi request reprint Gene organization of the mouse pro-hormone and pro-protein convertase PC1
    N Ftouhi
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    DNA Cell Biol 13:395-407. 1994
    ..Using a 3' end probe specifically hybridizing with the 5-kb mRNA, we show that in the mouse pituitary neurointermediate lobe the 3-kb form is negatively regulated by dopamine, while the 5-kb form is not...
  31. ncbi request reprint Comparative functional role of PC7 and furin in the processing of the HIV envelope glycoprotein gp160
    E Decroly
    J A DeSève Laboratory of Biochemical Neuroendocrinology, University of Montreal, QC, Canada
    FEBS Lett 405:68-72. 1997
    ..The data demonstrate that PC7 can cleave specifically and in a cell-type specific manner gp160 into gp120gp41, suggesting that both furin and PC7 are so far the major PC-like candidate gp160 convertase in T4 lymphocytes...
  32. ncbi request reprint Identification and localization of 7B2 protein in human, porcine, and rat thyroid gland and in human medullary carcinoma
    M Marcinkiewicz
    J A de Sève Laboratories of Biochemical and Molecular Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    Endocrinology 123:866-73. 1988
    ..Results suggest that 7B2 may play a role in endocrine function, possibly as a secretory substance, and may be a histochemical marker in addition to calcitonin for medullary carcinoma...
  33. ncbi request reprint Chromosomal assignments of the genes for neuroendocrine convertase PC1 (NEC1) to human 5q15-21, neuroendocrine convertase PC2 (NEC2) to human 20p11.1-11.2, and furin (mouse 7[D1-E2] region)
    N G Seidah
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    Genomics 11:103-7. 1991
    ..Furthermore, the identification of the NEC1 locus on human chromosome 5 and mouse chromosome 13 suggests a conservation of synthenic regions between these regions of the human and mouse genomes...
  34. ncbi request reprint Prosomatostatin is proteolytically processed at the amino terminal segment by subtilase SKI-1
    R Mouchantaf
    Fraser Laboratories, Department of Medicine, Room M3 15, Royal Victoria Hospital, McGill University, 687 Pine Avenue West, Montreal, Quebec, Canada H3A 1A1
    Regul Pept 120:133-40. 2004
    ..Mutagenesis studies showed that efficient processing of PSST to PSST[1-10] required the RXRXXL motif. However, this NH2-terminal cleavage was not a prerequisite for the formation of SST-14 and SST-28...
  35. ncbi request reprint The LIM homeobox protein mLIM3/Lhx3 induces expression of the prolactin gene by a Pit-1/GHF-1-independent pathway in corticotroph AtT20 cells
    S E Girardin
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Que, Canada
    FEBS Lett 431:333-8. 1998
    ..We conclude that mLIM3 may play a key role in inducing PRL gene expression in lactotrophs by binding to a conserved motif close to a Pit-1/GHF-1 site within the proximal PRL promoter...
  36. pmc Molecular cloning and biochemical characterization of a new mouse testis soluble-zinc-metallopeptidase of the neprilysin family
    G Ghaddar
    Departement de Biochimie, Faculte de Medecine, Universite de Montreal, C P 6128, Succ Centre Ville, Montreal, Quebec, Canada H3C 3J7
    Biochem J 347:419-29. 2000
    ..This distribution of NL1 mRNA suggests that it could be involved in sperm formation or other processes related to fertility...
  37. pmc cDNA structure, tissue distribution, and chromosomal localization of rat PC7, a novel mammalian proprotein convertase closest to yeast kexin-like proteinases
    N G Seidah
    Clinical Research Institute of Montreal, Canada
    Proc Natl Acad Sci U S A 93:3388-93. 1996
    ..The gene for PC7 (Pcsk7) was mapped to mouse chromosome 9 by linkage analysis of an interspecific backcross DNA panel...
  38. ncbi request reprint Functional analysis of human PACE4-A and PACE4-C isoforms: identification of a new PACE4-CS isoform
    M Zhong
    J A DeSève Laboratories of Biochemical Neuroendocrinology Clinical Research Institute of Montreal, University of Montreal, QC, Canada
    FEBS Lett 396:31-6. 1996
    ..Microsequencing of the intracellular PACE4-CS protein in two cell lines revealed that it is proPACE4-CS with an N-terminal trimming reminiscent of the action of a dipeptidylpeptidase recognizing the motifs X-Ala and X-Pro...
  39. pmc Ontogeny of the prohormone convertases PC1 and PC2 in the mouse hypophysis and their colocalization with corticotropin and alpha-melanotropin
    M Marcinkiewicz
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    Proc Natl Acad Sci U S A 90:4922-6. 1993
    ..It is expected that beta-endorphin processing will follow that of alpha MSH...
  40. pmc Comparative cellular processing of the human immunodeficiency virus (HIV-1) envelope glycoprotein gp160 by the mammalian subtilisin/kexin-like convertases
    F Vollenweider
    J A DeSève Laboratories of Biochemical and Molecular Neuroendocrinology, Montreal, Quebec, Canada
    Biochem J 314:521-32. 1996
    ..Finally, the co-expression of the [Arg355, Arg358]-alpha-1-antitrypsin Portland variant was shown to potently inhibit the processing of both gp160 and gp120 by these convertases...
  41. ncbi request reprint Chromogranin B (secretogranin I), a putative precursor of two novel pituitary peptides through processing at paired basic residues
    S Benjannet
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    FEBS Lett 224:142-8. 1987
    ..These data are thus in favor of a putative role of chromogranins as precursors to potentially bioactive peptides...
  42. ncbi request reprint Proprotein conversion is determined by a multiplicity of factors including convertase processing, substrate specificity, and intracellular environment. Cell type-specific processing of human prorenin by the convertase PC1
    S Benjannet
    J A DeSève Laboratory of Molecular Neuroendocrinology, University of Montreal, Quebec, Canada
    J Biol Chem 267:11417-23. 1992
    ..Our data demonstrate that propeptide removal from these enzymes, possibly leading to their activation, is not the only criterion which governs precursor processing...
  43. pmc pH-induced conformational transitions of a molten-globule-like state of the inhibitory prodomain of furin: implications for zymogen activation
    S Bhattacharjya
    Biomolecular Nuclear Magnetic Resonance Laboratory, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada
    Protein Sci 10:934-42. 2001
    ....
  44. ncbi request reprint The cDNA structure of rat plasma kallikrein
    N G Seidah
    Laboratory of Biochemical Neuronendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    DNA 8:563-74. 1989
    ....
  45. ncbi request reprint The neuroendocrine precursor 7B2 is a sulfated protein proteolytically processed by a ubiquitous furin-like convertase
    L Paquet
    Laboratory of Structure and Metabolism of Neuropeptides, Clinical Research Institute of Montreal, University of Montreal, Quebec, Canada
    J Biol Chem 269:19279-85. 1994
    ..These results demonstrate that pro7B2 is first processed by a furin-like enzyme within the trans-Golgi network into a 23-kDa form that is then sequestered into secretory granules...
  46. ncbi request reprint The cDNA sequence of the human pro-hormone and pro-protein convertase PC1
    N G Seidah
    J A DeSève Laboratory of Biochemical, Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    DNA Cell Biol 11:283-9. 1992
    ..2 kb and the presence of smaller transcripts in some tissues. The major site of production of hPC1 seems to be the pituitary and brain, although detection was also possible in pancreas and heart...
  47. ncbi request reprint 7B2 is a specific intracellular binding protein of the prohormone convertase PC2
    S Benjannet
    J A DeSève Laboratories of Molecular, Clinical Research Institute of Montreal, Quebec, Canada
    J Neurochem 64:2303-11. 1995
    ..Coexpression of 7B2 and PC2, although resulting in an elevation of the level of pro-PC2, did not eliminate the processing of pro-PC2 to PC2.(ABSTRACT TRUNCATED AT 250 WORDS)..
  48. ncbi request reprint Prohormone convertases in mouse submandibular gland: co-localization of furin and nerve growth factor
    H Farhadi
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Quebec, Canada
    J Histochem Cytochem 45:795-804. 1997
    ..These data, taken together with those from previous studies, suggest that furin is a candidate processing enzyme for NGF in mouse submandibular glands...
  49. ncbi request reprint Molecular cloning and expression of the ovine testicular follicle stimulating hormone receptor
    T A Yarney
    Reproduction Research Laboratory, Clinical Research Institute of Montreal, Quebec, Canada
    Mol Cell Endocrinol 93:219-26. 1993
    ..Chemically deglycosylated oFSH (DG-oFSH) was inactive in these cells but it effectively blocked the action of native hormone.(ABSTRACT TRUNCATED AT 250 WORDS)..
  50. pmc Comparative analysis of expression of the proprotein convertases furin, PACE4, PC1 and PC2 in human lung tumours
    M Mbikay
    Institut de Recherches Cliniques de Montreal, Universite de Montreal, Quebec, Canada
    Br J Cancer 75:1509-14. 1997
    ....
  51. ncbi request reprint Electron microscopic immunocytochemical evidence for the involvement of the convertases PC1 and PC2 in the processing of proinsulin in pancreatic beta-cells
    D Malide
    Department of Anatomy, Universite de Montreal, Quebec, Canada
    J Histochem Cytochem 43:11-9. 1995
    ..Co-expression of PC1 and PC2 with proinsulin in islet beta-cells indicates that these proteases are actively involved, probably in a sequential manner, in the conversion of proinsulin into insulin...
  52. ncbi request reprint Biosynthesis and enzymatic characterization of human SKI-1/S1P and the processing of its inhibitory prosegment
    B B Touré
    Laboratory of Biochemical, Protein Engineering Network of Centres of Excellence, Clinical Research Institute of Montreal, University of Montreal, Montreal, Quebec H2W 1R7
    J Biol Chem 275:2349-58. 2000
    ..Finally, we demonstrate that, in vitro, recombinant prosegments of SKI-1 inhibit its activity with apparent inhibitor constants of 100-200 nM...
  53. ncbi request reprint Processing of prodynorphin by the prohormone convertase PC1 results in high molecular weight intermediate forms. Cleavage at a single arginine residue
    A Dupuy
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    FEBS Lett 337:60-5. 1994
    ..Additionally, PC1 cleaves proDyn at a single arginine residue to yield an 8 kDa product and the C-peptide. This demonstrates that PC1 cleaves proDyn at single and pairs of basic residues...
  54. ncbi request reprint Histidine-rich human salivary peptides are inhibitors of proprotein convertases furin and PC7 but act as substrates for PC1
    A Basak
    Laboratory of Structure and Metabolism of Neuropeptides, Clinical Research Institute of Montreal, Quebec, Canada
    J Pept Res 49:596-603. 1997
    ..When tested against rPC7, H3 exhibited almost identical inhibition profile with a measured Ki of 2.4 microM. The partial sequence identity of H3 to the inhibitory pro-peptide of furin and PC7 provides a rationale for our observation...
  55. pmc Heterologous processing of rat prosomatostatin to somatostatin-14 by PC2: requirement for secretory cell but not the secretion granule
    A S Galanopoulou
    Fraser Laboratories, McGill University Department of Medicine, Royal Victoria Hospital, Montreal, Quebec, Canada
    Biochem J 311:111-8. 1995
    ..The activity of PC2 requires the milieu of the secretory cell but not the secretory granule...
  56. ncbi request reprint Secretory protein 7B2 is associated with pancreatic hormones within normal islets and some experimentally induced tumors
    S Benjannet
    J A de Sève Laboratories of Molecular and Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    Endocrinology 123:874-84. 1988
    ..In addition, IR-7B2 could be detected immunocytochemically in three of seven tumors produced in the rat by streptozotocin-nicotinamide treatment...
  57. ncbi request reprint Processed forms of neuroendocrine proteins 7B2 and secretogranin II are found in porcine pituitary extracts
    C Lazure
    J A De Sève Laboratory of Molecular, Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada, University of Montreal
    Int J Pept Protein Res 38:392-400. 1991
    ....
  58. ncbi request reprint The mouse homeoprotein mLIM-3 is expressed early in cells derived from the neuroepithelium and persists in adult pituitary
    N G Seidah
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada
    DNA Cell Biol 13:1163-80. 1994
    ..These data suggest a potential role for mLIM-3 in the transcriptional regulation of certain genes during morphogenesis and/or maintenance of the differentiated state of the pituitary, motor neurons, and pineal gland...
  59. ncbi request reprint Inhibitory activity and structural characterization of a C-terminal peptide fragment derived from the prosegment of the proprotein convertase PC7
    S Bhattacharjya
    Biomolecular NMR Laboratory, Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec, Canada H4P 2R2
    Biochemistry 39:2868-77. 2000
    ..We surmise that such a structure-function correlation for an inhibitory peptide could lead to the design and discovery of molecules mimicking the specific interactions of the PC prosegments for their cognate proteases...
  60. ncbi request reprint Pilocarpine-induced seizures are accompanied by a transient elevation in the messenger RNA expression of the prohormone convertase PC1 in rat hippocampus: comparison with nerve growth factor and brain-derived neurotrophic factor expression
    M Marcinkiewicz
    J A DeSève Laboratory of Molecular Neuroendocrinology, University ofMontreal, Quebec, Canada
    Neuroscience 76:425-39. 1997
    ..Our findings suggest the involvement of PC1 in the processing of precursor proteins during seizure activity...
  61. ncbi request reprint Developmental expression of the prohormone convertases PC1 and PC2 in mouse pancreatic islets
    M Marcinkiewicz
    J A DeSève Laboratories of Molecular Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    Endocrinology 135:1651-60. 1994
    ....
  62. pmc Processing of alpha4 integrin by the proprotein convertases: histidine at position P6 regulates cleavage
    Eric Bergeron
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, Canada, H2W 1R7
    Biochem J 373:475-84. 2003
    ..In conclusion, although the accepted furin processing motif is Arg-Xaa-(Lys/Arg)-Arg downward arrow, our data further extend it to include a regulatory histidine residue at P6 in precursors that lack a basic residue at P4...
  63. ncbi request reprint The regulated cell surface zymogen activation of the proprotein convertase PC5A directs the processing of its secretory substrates
    Gaetan Mayer
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 283:2373-84. 2008
    ..A similar mechanism may also apply to the convertase PACE4, thereby extending our knowledge of the molecular details of the zymogen activation and functions of these heparan sulfate proteoglycan-bound convertases...
  64. ncbi request reprint Prohormone convertase PC5 is a candidate processing enzyme for prorenin in the human adrenal cortex
    C Mercure
    Laboratory of Molecular Biochemistry of Hypertension, Clinical Research Institute of Montreal, Quebec, Canada
    Hypertension 28:840-6. 1996
    ..Human PC5 was colocalized with renin by immunohistochemistry in the zona glomerulosa of the adrenal gland, suggesting that it could participate in the activation of a local renin-angiotensin system in the human adrenal cortex...
  65. ncbi request reprint Post-translational processing of beta-secretase (beta-amyloid-converting enzyme) and its ectodomain shedding. The pro- and transmembrane/cytosolic domains affect its cellular activity and amyloid-beta production
    S Benjannet
    Biochemical Neuroendocrinology, , Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 276:10879-87. 2001
    ..These findings suggest that the shedding of BACE may play a role in the amyloidogenic processing of betaAPP...
  66. ncbi request reprint Processing specificity and biosynthesis of the Drosophila melanogaster convertases dfurin1, dfurin1-CRR, dfurin1-X, and dfurin2
    I De Bie
    J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    J Biol Chem 270:1020-8. 1995
    ..The conclusions drawn from the results of this study may well be applicable to the mammalian convertases PC4, PACE4, and PC5, which also display C-terminal sequence heterogeneity...
  67. ncbi request reprint Heparin enhances the furin cleavage of HIV-1 gp160 peptides
    A Pasquato
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada H2W 1R7
    FEBS Lett 581:5807-13. 2007
    ..Our data support the hypothesis of a direct binding of heparin with site1 and site2, allowing selective exposure/accessibility of the REKR sequence, which is only then optimally cleaved by furin...
  68. ncbi request reprint Two-dimensional immunoblotting analysis and immunocytochemical localization of the secretory polypeptide 7B2 in adrenal medulla
    M Marcinkiewicz
    J A de Sève Laboratories of Molecular and Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Que, Canada
    Neurosci Lett 95:81-7. 1988
    ..These data establish that in chromaffin granules 7B2 represents a minor component of the acidic proteins which include the chromogranins A and B, secretogranin II and the enkephalin-containing peptides...
  69. ncbi request reprint The production by alternate splicing of two mRNAs differing by one codon could be an intrinsic property of neuroendocrine protein 7B2 gene expression in man
    L Paquet
    Laboratoires J A de Sève de Neuroendocrinologie Moléculaire et Biochimique, Institut de Recherches Cliniques de Montreal, Quebec, Canada
    Biochem Biophys Res Commun 174:156-62. 1991
    ....
  70. ncbi request reprint The proteolytic processing of pro-platelet-derived growth factor-A at RRKR(86) by members of the proprotein convertase family is functionally correlated to platelet-derived growth factor-A-induced functions and tumorigenicity
    Geraldine Siegfried
    Laboratory of Biochemical Neuroendocrinology Clinical Research Institute of Montreal, Montreal, Quebec, H2W 1R7, Canada
    Cancer Res 63:1458-63. 2003
    ..Chinese hamster ovary cells overexpressing pro-PDGF-A (ARKA(86)) failed to induce tumors in nude mice. Thus, PC-directed inhibitors might represent new agents for therapy in neoplasia induced by PDGF-A...
  71. ncbi request reprint Gene structure and chromosomal localization of plasma kallikrein
    G Beaubien
    J A DeSève Laboratories of Biochemical and Molecular Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
    Biochemistry 30:1628-35. 1991
    ....
  72. ncbi request reprint Eukaryotic protein processing: endoproteolysis of precursor proteins
    N G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, QC, Canada
    Curr Opin Biotechnol 8:602-7. 1997
    ....
  73. ncbi request reprint Testicular expression of PC4 in the rat: molecular diversity of a novel germ cell-specific Kex2/subtilisin-like proprotein convertase
    N G Seidah
    J A DeSève Laboratories of Biochemical, Clinical Research Institute of Montreal, Quebec, Canada
    Mol Endocrinol 6:1559-70. 1992
    ..The stage-specific expression of PC4 in testis indicates its potential role in the developmental maturation of germ cells and that this convertase may play a specific physiological function in reproduction...
  74. ncbi request reprint Coordinated expression of beta-amyloid precursor protein and the putative beta-secretase BACE and alpha-secretase ADAM10 in mouse and human brain
    M Marcinkiewicz
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada
    J Neurochem 75:2133-43. 2000
    ..Our data provide cytochemical evidence supporting the role of ADAM10 and BACE as authentic alpha- and beta-secretases...
  75. pmc The secretory proprotein convertases furin, PC5, and PC7 activate VEGF-C to induce tumorigenesis
    Geraldine Siegfried
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada
    J Clin Invest 111:1723-32. 2003
    ..Our findings demonstrate the processing of proVEGF-C by PCs and highlight the potential use of PC inhibitors as agents for inhibiting malignancies induced by VEGF-C...
  76. ncbi request reprint NH2-terminal fragment of rat pro-atrial natriuretic factor in the circulation: identification, radioimmunoassay and half-life
    G Thibault
    Clinical Research Institute of Montreal, Quebec, Canada
    Peptides 9:47-53. 1988
    ..The exponential decay was analyzed by a two-compartment model in which the fast and slow components had a half-life of 2.5 +/- 0.3 and 54.8 +/- 3.9 min, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)..
  77. pmc The cysteine-rich domain of the secreted proprotein convertases PC5A and PACE4 functions as a cell surface anchor and interacts with tissue inhibitors of metalloproteinases
    Nadia Nour
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    Mol Biol Cell 16:5215-26. 2005
    ..In conclusion, the CRD of PC5A and PACE4 functions as a cell surface anchor favoring the processing of their cognate surface-anchored substrates, including endothelial lipase...
  78. doi request reprint Proprotein convertase subtilisin/kexin type 9 (PCSK9): hepatocyte-specific low-density lipoprotein receptor degradation and critical role in mouse liver regeneration
    Ahmed Zaid
    Laboratorie of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, affiliated to the University of Montreal, Montreal, Quebec, Canada
    Hepatology 48:646-54. 2008
    ..However, lipid accumulation in hepatocytes of these mice was markedly reduced under both chow and high-cholesterol diets, revealing that PCSK9 deficiency confers resistance to liver steatosis...
  79. ncbi request reprint Development of protein-based inhibitors of the proprotein of convertase SKI-1/S1P: processing of SREBP-2, ATF6, and a viral glycoprotein
    Philomena Pullikotil
    Laboratories of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 279:17338-47. 2004
    ..Finally, these inhibitors were also shown to affect the processing and stability of the Crimean-Congo hemorrhagic fever virus glycoprotein...
  80. ncbi request reprint NARC-1/PCSK9 and its natural mutants: zymogen cleavage and effects on the low density lipoprotein (LDL) receptor and LDL cholesterol
    Suzanne Benjannet
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 279:48865-75. 2004
    ..In conclusion, NARC-1 seems to affect both the level of LDLR and that of circulating apoB-containing lipoproteins in an LDLR-dependent and -independent fashion...
  81. ncbi request reprint Statins upregulate PCSK9, the gene encoding the proprotein convertase neural apoptosis-regulated convertase-1 implicated in familial hypercholesterolemia
    Geneviève Dubuc
    Laboratory of Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montreal, Quebec, Canada
    Arterioscler Thromb Vasc Biol 24:1454-9. 2004
    ..The 2 other known genes implicated in ADH encode the low-density lipoprotein receptor and apolipoprotein B. As an approach toward the elucidation of the physiological role(s) of NARC-1, we studied its transcriptional regulation...
  82. ncbi request reprint The proprotein convertase PCSK9 induces the degradation of low density lipoprotein receptor (LDLR) and its closest family members VLDLR and ApoER2
    Steve Poirier
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 283:2363-72. 2008
    ..Thus, this study demonstrates a more general effect of PCSK9 on the degradation of the LDLR family that emphasizes its major role in cholesterol and lipid homeostasis as well as brain development...
  83. pmc Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route
    Steve Poirier
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 284:28856-64. 2009
    ..Therefore, targeting either pathway, or both, would be an effective method to reduce PCSK9 activity in the treatment of hypercholesterolemia and coronary heart disease...
  84. ncbi request reprint The proprotein convertase SKI-1/S1P. In vitro analysis of Lassa virus glycoprotein-derived substrates and ex vivo validation of irreversible peptide inhibitors
    Antonella Pasquato
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 281:23471-81. 2006
    ....
  85. ncbi request reprint The cellular trafficking of the secretory proprotein convertase PCSK9 and its dependence on the LDLR
    Nasha Nassoury
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada H2W 1R7
    Traffic 8:718-32. 2007
    ..Mutations that affect this transport can lead to hyper- or hypocholesterolemia...
  86. pmc Deletion of the gene encoding proprotein convertase 5/6 causes early embryonic lethality in the mouse
    Rachid Essalmani
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada
    Mol Cell Biol 26:354-61. 2006
    ..5 and 7.5. These data demonstrate that Pcsk5 is an essential gene...
  87. ncbi request reprint The proprotein convertases and their implication in sterol and/or lipid metabolism
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal H2W 1R7, QC, Canada
    Biol Chem 387:871-7. 2006
    ..Finally, PCSK9 downregulates the protein levels of the LDLR by enhancement of its intracellular metabolic pathway in subcellular acidic compartments...
  88. doi request reprint Annexin A2 is a C-terminal PCSK9-binding protein that regulates endogenous low density lipoprotein receptor levels
    Gaetan Mayer
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 283:31791-801. 2008
    ..The identification of the minimal inhibitory sequence of AnxA2 should pave the way toward the development of PCSK9 inhibitory lead molecules for the treatment of hypercholesterolemia...
  89. doi request reprint Circulating proprotein convertase subtilisin/kexin 9 (PCSK9) regulates VLDLR protein and triglyceride accumulation in visceral adipose tissue
    Anna Roubtsova
    Laboratory of Biochemical Neuroendocrinology, University of Montreal, Montreal, Quebec, Canada
    Arterioscler Thromb Vasc Biol 31:785-91. 2011
    ..Herein, we investigated the role of PCSK9 in adipose tissue metabolism...
  90. ncbi request reprint The influence of PCSK9 polymorphisms on serum low-density lipoprotein cholesterol and risk of atherosclerosis
    Jean Davignon
    Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montreal IRCM, 110 Pine Avenue West, Montreal, QC, H2W 1R7, Canada
    Curr Atheroscler Rep 12:308-15. 2010
    ..Which one will be clinically applicable will depend on long-term effects, cost, and ease of administration...
  91. pmc Proprotein convertases in tumor progression and malignancy: novel targets in cancer therapy
    Abdel Majid Khatib
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada
    Am J Pathol 160:1921-35. 2002
    ..Thus, PC inhibitors may constitute new promising agents for the treatment of multiple tumors and/or in adjuvant therapy to prevent recurrence...
  92. ncbi request reprint Biosynthesis and cellular trafficking of the convertase SKI-1/S1P: ectodomain shedding requires SKI-1 activity
    Aram Elagoz
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 277:11265-75. 2002
    ..In conclusion, multiple domains ensuring optimal functional characteristics control SKI-1 activity and cellular trafficking...
  93. ncbi request reprint Structure-function analysis of the prosegment of the proprotein convertase PC5A
    Nadia Nour
    Laboratories of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 278:2886-95. 2003
    ..In conclusion, cleavages of the prosegment of PC5A at both Arg(116) and Arg(84) are required for PC5A cellular activity, and ppPC5 is a very potent but modestly selective cellular inhibitor of PC5A...
  94. ncbi request reprint Beta-amyloid protein converting enzyme 1 and brain-specific type II membrane protein BRI3: binding partners processed by furin
    Louise Wickham
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada
    J Neurochem 92:93-102. 2005
    ..Thus, furin efficiently processes both pro-BACE1 and its novel interacting protein pro-BRI3...
  95. ncbi request reprint Implication of the proprotein convertase NARC-1/PCSK9 in the development of the nervous system
    Steve Poirier
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada
    J Neurochem 98:838-50. 2006
    ..These data support a novel role for NARC-1/PCSK9 in CNS development, distinct from that in cholesterogenic organs such as liver...
  96. ncbi request reprint Implication of proprotein convertases in the processing and spread of severe acute respiratory syndrome coronavirus
    Eric Bergeron
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Que, Canada H2W 1R7
    Biochem Biophys Res Commun 326:554-63. 2005
    ..Taken together, our data indicate that furin or PC-mediated processing plays a critical role in SARS-CoV spread and cytopathicity, and inhibitors of the PCs represent potential therapeutic anti-SARS-CoV agents...
  97. ncbi request reprint The proprotein convertase (PC) PCSK9 is inactivated by furin and/or PC5/6A: functional consequences of natural mutations and post-translational modifications
    Suzanne Benjannet
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 281:30561-72. 2006
    ..The latter may reduce the lifetime of this proteinase and its ability to degrade the cell-surface LDL receptor, thereby regulating the levels of circulating LDL cholesterol...
  98. pmc The metalloendopeptidase nardilysin (NRDc) is potently inhibited by heparin-binding epidermal growth factor-like growth factor (HB-EGF)
    Véronique Hospital
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    Biochem J 367:229-38. 2002
    ..We conclude that the DAC is thus a key regulatory element for the two distinct functions that NRDc fulfills, i.e. as an HB-EGF modulator and a peptidase...
  99. ncbi request reprint PC1/3, PC2 and PC5/6A are targeted to dense core secretory granules by a common mechanism
    Jimmy D Dikeakos
    Laboratory of Molecular Biochemistry of Hypertension, Institut de recherches cliniques de Montreal IRCM, QC, Canada
    FEBS J 274:4094-102. 2007
    ..Analysis of the predicted structures formed by these three granule-sorting helices shows a correlation between their granule-sorting efficiency and the clustering of hydrophobic amino acids in their granule-targeting helices...
  100. ncbi request reprint Evidence for proprotein convertase activity in the endoplasmic reticulum/early Golgi
    Alexandre Salvas
    Biochemical Neuroendocrinology Laboratory, Clinical Research Institute of Montreal, QC, Canada
    FEBS Lett 579:5621-5. 2005
    ..This suggests that the convertases could cleave some substrates within the ER, possibly by displacing the inhibitory prosegment associated with them...
  101. pmc In vivo functions of the proprotein convertase PC5/6 during mouse development: Gdf11 is a likely substrate
    Rachid Essalmani
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, Canada
    Proc Natl Acad Sci U S A 105:5750-5. 2008
    ..This work identifies Gdf11 as a likely in vivo specific substrate of PC5/6 and opens the way to the identification of other key substrates of this convertase...

Research Grants1

  1. Proprotein Processing, Trafficking and Secretion Gordon Conference
    Nabil Seidah; Fiscal Year: 2006
    ..The integration of protease systems that are required both for normal physiology and human disease will be relevant to our understanding of the molecular bases of diabetes, cancer, Alzheimer's and drug addiction. ..