Affiliation: Children's Hospital of Eastern Ontario
- Evaluation of the QuickLab RSV test, a new rapid lateral-flow immunoassay for detection of respiratory syncytial virus antigenRobert Slinger
Division of Infectious Disease, Children s Hospital of Eastern Ontario, 401 Smyth Rd, Ottawa, Ontario K1H 8L1, Canada
J Clin Microbiol 42:3731-3. 2004..001). We conclude that the QuickLab RSV test has sensitivity similar to that of the DFA assay and better than that of the DIR assay. QuickLab testing is also simpler to perform and interpret than both DFA and DIR testing...
- Direct Streptococcus pneumoniae real-time PCR serotyping from pediatric parapneumonic effusionsRobert Slinger
Department of Laboratory Medicine and Pathology and Department of Pediatrics, University of Ottawa, Ottawa, ON, Canada
BMC Pediatr 14:189. 2014..To determine the serotypes of Streptococcus pneumoniae responsible for pneumonia complicated by parapneumonic effusion in children, we performed real-time PCR based pneumococcal "serotyping" directly on parapneumonic fluid samples...
- Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, CanadaDavid M Goldfarb
Department of Pediatrics, McMaster University, Hamilton, ON, Canada
Int J Circumpolar Health 72:19903. 2013..There are a number of limitations of conventional microbiology testing techniques for diarrhoeal pathogens, and these may be further compromised in the Arctic, given the often long distances for specimen transport...
- Successful nanolitre real-time PCR detection of respiratory pathogens in clinical specimensR Slinger
Children s Hospital of Eastern Ontario, Ottawa, ON, Canada
Clin Microbiol Infect 18:E286-8. 2012..RSV nanolitre PCR was also 100% sensitive (21/21; 95% CI, 85-100%) and specific (25/25; 95%, CI 87-100%). Respiratory pathogens can be successfully detected in clinical specimens using nanolitre-volume PCR...
- Rapid detection of Bordetella pertussis and Bordetella parapertussis in clinical and molecular proficiency panel specimens with a novel intercalating dye-based PCR assayRobert Slinger
Children s Hospital of Eastern Ontario, University of Ottawa, Ottawa, Ontario, Canada
Scand J Infect Dis 43:968-71. 2011..The assay can be performed in 1 h including DNA extraction. The new assay thus offers a simple and rapid alternative for the detection of B. pertussis and B. parapertussis...
- Cloxacillin versus vancomycin for presumed late-onset sepsis in the Neonatal Intensive Care Unit and the impact upon outcome of coagulase negative staphylococcal bacteremia: a retrospective cohort studySarah L Lawrence
Department of Obstetrics, Gynecology and Newborn Care, The Ottawa Hospital, Ottawa, Ontario, Canada
BMC Pediatr 5:49. 2005..The objective of this study was to compare duration of CONS sepsis and mortality before and after implementation of a policy of selective vancomycin use and compare use of vancomycin between the 2 time periods...
- Rapid PCR detection of group A Streptococcus from flocked throat swabs: a retrospective clinical studyRobert Slinger
Department of Laboratory Medicine, Children s Hospital of Eastern Ontario, University of Ottawa, Ontario, Canada
Ann Clin Microbiol Antimicrob 10:33. 2011..Very few molecular methods for detection of GAS in clinical throat swab specimens have been described...
- Pyrosequencing assay to rapidly detect clarithromycin resistance mutations in Canadian Helicobacter pylori isolatesR Slinger
Children s Hospital of Eastern Ontario, University of Ottawa, Ottawa, ON, Canada
Can J Gastroenterol 23:609-12. 2009..Mutations at positions 2142 or 2143 in the twocopy 23S ribosomal RNA gene of Helicobacter pylori are highly predictive of in vitro clarithromycin resistance and failure of clarithromycin-containing treatment regimens...
- Transfusion-transmitted malaria in CanadaR Slinger
Centre for Infectious Disease Prevention and Control, Population and Public Health Branch, Health Canada, Ottawa, Ont
CMAJ 164:377-9. 2001..Although very rare, this diagnosis should be considered in transfusion recipients who have undiagnosed symptoms consistent with malaria. Thick and thin blood smears should be urgently examined to exclude this possibility...
- Pyrosequencing of a recA gene variable region for Burkholderia cepacia complex genomovar identificationRobert Slinger
Department of Pediatrics, University of Ottawa, Ottawa, Ontario, Canada K1H 8MS
Diagn Microbiol Infect Dis 58:379-84. 2007..Three Burkholderia multivorans isolates were identified as B. multivorans/Burkholderia ambifaria, indicating that additional identification methods may be needed for some B. multivorans strains...
- High-resolution melting assay for the detection of gyrA mutations causing quinolone resistance in Salmonella enterica serovars Typhi and ParatyphiRobert Slinger
Division of Bacteriology, Department of Pathology and Laboratory Medicine, Children s Hospital of Eastern Ontario, Ottawa, ON, Canada K1H 8L1
Diagn Microbiol Infect Dis 57:455-8. 2007..The presence of gyrA mutations led to small but consistent changes in amplicon melting temperatures that allowed quinolone-resistant isolates to be differentiated from susceptible ones...
- Multiple combination antibiotic susceptibility testing of nontypeable Haemophilus influenzae biofilmsRobert Slinger
Department of Pediatrics, Ottawa, Ontario, Canada K1H 8L1
Diagn Microbiol Infect Dis 56:247-53. 2006..Biofilm testing reveals differences in effectiveness among antibiotics not apparent from conventional susceptibility testing, and suggests novel antibiotic regimens that could be studied for treatment of OME...
- Suboptimal clinical response to ciprofloxacin in patients with enteric fever due to Salmonella spp. with reduced fluoroquinolone susceptibility: a case seriesRobert Slinger
Division of Infectious Disease, Children s Hospital of Eastern Ontario, 401 Smyth Rd, Ottawa, ON, Canada
BMC Infect Dis 4:36. 2004..Delayed treatment response to fluoroquinolones has been noted, especially in cases of enteric fever due to such strains. We reviewed the ciprofloxacin susceptibility and clinical outcome of our recent enteric fever cases...
- Infection with Burkholderia cepacia complex bacteria and pulmonary exacerbations of cystic fibrosisMelissa St Denis
The Ottawa Hospital, General Campus, 501 Smyth Rd, Ottawa, ON, Canada
Chest 131:1188-96. 2007..Our objective was to determine whether pulmonary exacerbations of CF are associated with acquisition of new B cepacia strains or with B cepacia strain replacement...
- Assessment of flocked swabs for use in identification of streptococcal pharyngitisDavid M Goldfarb
Division of Infectious Diseases, Children s Hospital of Eastern Ontario, University of Ottawa, Ontario, Canada
J Clin Microbiol 47:3029-30. 2009..Sensitivity was higher for flocked swabs, but this did not reach statistical significance. We conclude that flocked swabs can be used in place of traditional swabs for diagnosis of streptococcal pharyngitis...
- Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilmsTalal Alandejani
Department of Otolaryngology, University of Ottawa, and Childrens Hospital of Eastern Ontario, Ottawa, Ontario, Canada
Otolaryngol Head Neck Surg 141:114-8. 2009..As well, honey has been used as an effective topical antimicrobial agent for years. Our objective is to determine the in vitro effect of honey against biofilms produced by PA and SA...
- Tache noireNick Daneman
Department of Medicine, Infectious Diseases, Sunnybrook Health Sciences Centre, Toronto, Ont
CMAJ 178:841. 2008