Affiliation: Canadian Forest Service
- Characterization of an AGAMOUS homologue from the conifer black spruce (Picea mariana) that produces floral homeotic conversions when expressed in ArabidopsisR Rutledge
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Sainte Foy, Quebec, Canada
Plant J 15:625-34. 1998..This suggests that some of the genetic pathways controlling flower and cone development are homologous, and antedate the 300-million-year-old divergence of angiosperms and gymnosperms...
- Mathematics of quantitative kinetic PCR and the application of standard curvesR G Rutledge
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, PO Box 3800, Sainte Foy, Quebec G1V 4C7, Canada
Nucleic Acids Res 31:e93. 2003..A simplified method for absolute quantification is also proposed, in which quantitative scale is determined by DNA mass at threshold...
- An improved MUG fluorescent assay for the determination of GUS activity within transgenic tissue of woody plantsC Cote
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, P O Box 3800, G1V 4C7 Sainte Foy, Quebec, Canada
Plant Cell Rep 21:619-24. 2003..Normalization of GUS activity upon DNA concentration was also found to be an effective alternative to protein concentration, providing the ability to make cross-species and inter-tissue comparisons of gusA transgene activity...
- Transgene integration patterns and expression levels in transgenic tissue lines of Picea mariana, P glauca and P abiesK Klimaszewska
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, PO Box 3800, G1 V 4C7, Sainte Foy, Quebec, Canada
Plant Cell Rep 21:1080-7. 2003..The average level of GUS activity, in lines that integrated one gene copy, was the highest in white spruce followed by black spruce and Norway spruce (22.7, 16.5 and 6.3 nmol 4-methylumbelliferone min(-1 )mg(-1 )protein, respectively)...
- Isolation and expression of cDNA clones coding for two sequence variants of Xenopus laevis histone H5R G Rutledge
Molecular Genetics Section, National Research Council of Canada, Ottawa, Ontario
Gene 70:117-26. 1988..Further, the XLH5-coding mRNA is likely polyadenylated and lacks the highly conserved, 23-nucleotide dyad symmetry element found within the 3' untranslated regions of most histone-coding mRNAs...
- A complete complementary DNA for the oncodevelopmental calcium-binding protein, oncomodulinM F Gillen
J Biol Chem 262:5308-12. 1987..The oncomodulin gene appears to have diverged more from that of calmodulin than has the parvalbumin gene...
- Characterization of a MYBR2R3 gene from black spruce (Picea mariana) that shares functional conservation with maize C1B Xue
Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055 du P E P S, P O Box 3800, Sainte Foy, Quebec, G1V 4C7, Canada
Mol Genet Genomics 270:78-86. 2003....
- Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applicationsR G Rutledge
Natural Resources Canada, 1055 du P E P S, Sainte Foy, Quebec, Canada G1V 4C7
Nucleic Acids Res 32:e178. 2004..Combined with provision of quantitative scale via optical calibration, sigmoidal curve-fitting could confer the capability for fully automated quantification of nucleic acids with unparalleled accuracy and reliability...