R Rutledge

Summary

Affiliation: Canadian Forest Service
Country: Canada

Publications

  1. ncbi request reprint Characterization of an AGAMOUS homologue from the conifer black spruce (Picea mariana) that produces floral homeotic conversions when expressed in Arabidopsis
    R Rutledge
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Sainte Foy, Quebec, Canada
    Plant J 15:625-34. 1998
  2. pmc Mathematics of quantitative kinetic PCR and the application of standard curves
    R G Rutledge
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, PO Box 3800, Sainte Foy, Quebec G1V 4C7, Canada
    Nucleic Acids Res 31:e93. 2003
  3. ncbi request reprint An improved MUG fluorescent assay for the determination of GUS activity within transgenic tissue of woody plants
    C Cote
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, P O Box 3800, G1V 4C7 Sainte Foy, Quebec, Canada
    Plant Cell Rep 21:619-24. 2003
  4. ncbi request reprint Transgene integration patterns and expression levels in transgenic tissue lines of Picea mariana, P glauca and P abies
    K Klimaszewska
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, PO Box 3800, G1 V 4C7, Sainte Foy, Quebec, Canada
    Plant Cell Rep 21:1080-7. 2003
  5. ncbi request reprint Isolation and expression of cDNA clones coding for two sequence variants of Xenopus laevis histone H5
    R G Rutledge
    Molecular Genetics Section, National Research Council of Canada, Ottawa, Ontario
    Gene 70:117-26. 1988
  6. ncbi request reprint A complete complementary DNA for the oncodevelopmental calcium-binding protein, oncomodulin
    M F Gillen
    J Biol Chem 262:5308-12. 1987
  7. ncbi request reprint Characterization of a MYBR2R3 gene from black spruce (Picea mariana) that shares functional conservation with maize C1
    B Xue
    Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055 du P E P S, P O Box 3800, Sainte Foy, Quebec, G1V 4C7, Canada
    Mol Genet Genomics 270:78-86. 2003
  8. pmc Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
    R G Rutledge
    Natural Resources Canada, 1055 du P E P S, Sainte Foy, Quebec, Canada G1V 4C7
    Nucleic Acids Res 32:e178. 2004

Collaborators

  • K Klimaszewska
  • C Cote
  • B Xue
  • Y Devantier
  • P J Charest
  • M F Gillen
  • V L Seligy
  • D Banville
  • S Narang
  • J F Whitfield
  • J P MacManus

Detail Information

Publications8

  1. ncbi request reprint Characterization of an AGAMOUS homologue from the conifer black spruce (Picea mariana) that produces floral homeotic conversions when expressed in Arabidopsis
    R Rutledge
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Sainte Foy, Quebec, Canada
    Plant J 15:625-34. 1998
    ..This suggests that some of the genetic pathways controlling flower and cone development are homologous, and antedate the 300-million-year-old divergence of angiosperms and gymnosperms...
  2. pmc Mathematics of quantitative kinetic PCR and the application of standard curves
    R G Rutledge
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, PO Box 3800, Sainte Foy, Quebec G1V 4C7, Canada
    Nucleic Acids Res 31:e93. 2003
    ..A simplified method for absolute quantification is also proposed, in which quantitative scale is determined by DNA mass at threshold...
  3. ncbi request reprint An improved MUG fluorescent assay for the determination of GUS activity within transgenic tissue of woody plants
    C Cote
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P E P S, P O Box 3800, G1V 4C7 Sainte Foy, Quebec, Canada
    Plant Cell Rep 21:619-24. 2003
    ..Normalization of GUS activity upon DNA concentration was also found to be an effective alternative to protein concentration, providing the ability to make cross-species and inter-tissue comparisons of gusA transgene activity...
  4. ncbi request reprint Transgene integration patterns and expression levels in transgenic tissue lines of Picea mariana, P glauca and P abies
    K Klimaszewska
    Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, PO Box 3800, G1 V 4C7, Sainte Foy, Quebec, Canada
    Plant Cell Rep 21:1080-7. 2003
    ..The average level of GUS activity, in lines that integrated one gene copy, was the highest in white spruce followed by black spruce and Norway spruce (22.7, 16.5 and 6.3 nmol 4-methylumbelliferone min(-1 )mg(-1 )protein, respectively)...
  5. ncbi request reprint Isolation and expression of cDNA clones coding for two sequence variants of Xenopus laevis histone H5
    R G Rutledge
    Molecular Genetics Section, National Research Council of Canada, Ottawa, Ontario
    Gene 70:117-26. 1988
    ..Further, the XLH5-coding mRNA is likely polyadenylated and lacks the highly conserved, 23-nucleotide dyad symmetry element found within the 3' untranslated regions of most histone-coding mRNAs...
  6. ncbi request reprint A complete complementary DNA for the oncodevelopmental calcium-binding protein, oncomodulin
    M F Gillen
    J Biol Chem 262:5308-12. 1987
    ..The oncomodulin gene appears to have diverged more from that of calmodulin than has the parvalbumin gene...
  7. ncbi request reprint Characterization of a MYBR2R3 gene from black spruce (Picea mariana) that shares functional conservation with maize C1
    B Xue
    Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055 du P E P S, P O Box 3800, Sainte Foy, Quebec, G1V 4C7, Canada
    Mol Genet Genomics 270:78-86. 2003
    ....
  8. pmc Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
    R G Rutledge
    Natural Resources Canada, 1055 du P E P S, Sainte Foy, Quebec, Canada G1V 4C7
    Nucleic Acids Res 32:e178. 2004
    ..Combined with provision of quantitative scale via optical calibration, sigmoidal curve-fitting could confer the capability for fully automated quantification of nucleic acids with unparalleled accuracy and reliability...