Robert A Holt

Summary

Affiliation: British Columbia
Country: Canada

Publications

  1. pmc Constructing large DNA segments by iterative clone recombination
    Duane E Smailus
    Canada s Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Suite 100, 570 West 7th Avenue, Vancouver, BC, Canada, V5Z 4S6
    Syst Synth Biol 1:139-44. 2007
  2. pmc A physical map of the bovine genome
    Warren M Snelling
    USDA, ARS, US Meat Animal Research Center, Clay Center, NE 68933, USA
    Genome Biol 8:R165. 2007
  3. pmc LongSAGE profiling of nine human embryonic stem cell lines
    Martin Hirst
    Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3
    Genome Biol 8:R113. 2007
  4. pmc Identification of genes expressed in the hermaphrodite germ line of C. elegans using SAGE
    Xin Wang
    Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
    BMC Genomics 10:213. 2009
  5. pmc A salmonid EST genomic study: genes, duplications, phylogeny and microarrays
    Ben F Koop
    Centre for Biomedical Research, University of Victoria, Victoria, British Columbia V8W3N5, Canada
    BMC Genomics 9:545. 2008
  6. pmc Salmo salar and Esox lucius full-length cDNA sequences reveal changes in evolutionary pressures on a post-tetraploidization genome
    Jong S Leong
    Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia, V8W 3N5 Canada
    BMC Genomics 11:279. 2010
  7. pmc Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters
    Nathalie Pavy
    Pavillon Charles Eugène Marchand, Universite Laval, Ste Foy, Quebec G1K 7P4, Canada
    BMC Genomics 6:144. 2005
  8. ncbi request reprint Rebuilding microbial genomes
    Robert A Holt
    Canada s Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada
    Bioessays 29:580-90. 2007
  9. doi request reprint The new paradigm of flow cell sequencing
    Robert A Holt
    British Columbia Cancer Agency, Genome Sciences Centre, Vancouver, British Columbia V5Z 4E6, Canada
    Genome Res 18:839-46. 2008
  10. pmc Efficient assembly of very short oligonucleotides using T4 DNA Ligase
    Daniel R Horspool
    British Columbia Cancer Agency, Genome Sciences Centre, Suite 100, 570 West 7th Avenue, Vancouver, British Columbia V5Z 4S6, Canada
    BMC Res Notes 3:291. 2010

Research Grants

Detail Information

Publications62

  1. pmc Constructing large DNA segments by iterative clone recombination
    Duane E Smailus
    Canada s Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Suite 100, 570 West 7th Avenue, Vancouver, BC, Canada, V5Z 4S6
    Syst Synth Biol 1:139-44. 2007
    ..Using this technique we rebuilt two non-contiguous regions of the Haemophilus influenzae genome as episomes in recombinogenic Escherichia coli host cells. These regions together comprise190 kbp, or 10.4% of the H. influenze genome...
  2. pmc A physical map of the bovine genome
    Warren M Snelling
    USDA, ARS, US Meat Animal Research Center, Clay Center, NE 68933, USA
    Genome Biol 8:R165. 2007
    ..The BAC libraries and clone maps are essential for the hybrid clone-by-clone/whole-genome shotgun sequencing approach taken by the bovine genome sequencing project...
  3. pmc LongSAGE profiling of nine human embryonic stem cell lines
    Martin Hirst
    Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3
    Genome Biol 8:R113. 2007
    ....
  4. pmc Identification of genes expressed in the hermaphrodite germ line of C. elegans using SAGE
    Xin Wang
    Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
    BMC Genomics 10:213. 2009
    ..To increase our understanding of the genes that might be necessary for the formation and function of the germ line, we have constructed a SAGE library from hand dissected C. elegans hermaphrodite gonads...
  5. pmc A salmonid EST genomic study: genes, duplications, phylogeny and microarrays
    Ben F Koop
    Centre for Biomedical Research, University of Victoria, Victoria, British Columbia V8W3N5, Canada
    BMC Genomics 9:545. 2008
    ..A comprehensive gene list and a comparison of genes in some of the different species provide valuable genomic information for one of the most widely studied groups of fish...
  6. pmc Salmo salar and Esox lucius full-length cDNA sequences reveal changes in evolutionary pressures on a post-tetraploidization genome
    Jong S Leong
    Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia, V8W 3N5 Canada
    BMC Genomics 11:279. 2010
    ....
  7. pmc Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters
    Nathalie Pavy
    Pavillon Charles Eugène Marchand, Universite Laval, Ste Foy, Quebec G1K 7P4, Canada
    BMC Genomics 6:144. 2005
    ..Our objective was to produce extensive collections of ESTs and cDNA clones to support manufacture of cDNA microarrays and gene discovery in white spruce (Picea glauca [Moench] Voss)...
  8. ncbi request reprint Rebuilding microbial genomes
    Robert A Holt
    Canada s Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada
    Bioessays 29:580-90. 2007
    ..influenzae and other microbial genome assemblies reveals few genes routinely incompatible with E. coli. In conclusion, rebuilding and booting a genome remains a feasible and pragmatic approach to creating a synthetic microbial cell...
  9. doi request reprint The new paradigm of flow cell sequencing
    Robert A Holt
    British Columbia Cancer Agency, Genome Sciences Centre, Vancouver, British Columbia V5Z 4E6, Canada
    Genome Res 18:839-46. 2008
    ..Given the ever increasing demand for DNA sequence information, we can expect continuous improvement of this new generation of instruments and their eventual replacement by even more powerful technology...
  10. pmc Efficient assembly of very short oligonucleotides using T4 DNA Ligase
    Daniel R Horspool
    British Columbia Cancer Agency, Genome Sciences Centre, Suite 100, 570 West 7th Avenue, Vancouver, British Columbia V5Z 4S6, Canada
    BMC Res Notes 3:291. 2010
    ..In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short oligonucleotide substrates and defined conditions suitable for assembly of a plurality of oligonucleotides...
  11. pmc A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)
    Steven G Ralph
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada
    BMC Genomics 9:484. 2008
    ..Sequence-finished full-length (FL)cDNAs and large collections of expressed sequence tags (ESTs) are essential for gene discovery, functional genomics, and for future efforts of conifer genome annotation...
  12. pmc Analysis of 4,664 high-quality sequence-finished poplar full-length cDNA clones and their utility for the discovery of genes responding to insect feeding
    Steven G Ralph
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
    BMC Genomics 9:57. 2008
    ..Poplar has been established as a model system for genomics studies of growth, development, and adaptation of woody perennial plants including secondary xylem formation, dormancy, adaptation to local environments, and biotic interactions...
  13. pmc Transcription of foreign DNA in Escherichia coli
    Rene L Warren
    BC Cancer Agency, Canada s Michael Smith Genome Sciences Centre, Vancouver, British Columbia V5Z 4S6, Canada
    Genome Res 18:1798-805. 2008
    ..Ongoing characterization of cross-expression will help inform the design of biosynthetic gene clusters and synthetic microbial genomes...
  14. pmc A regulatory toolbox of MiniPromoters to drive selective expression in the brain
    Elodie Portales-Casamar
    Centre for Molecular Medicine and Therapeutics at the Child and Family Research Institute, University of British Columbia, Vancouver, BC, Canada V5Z4H4
    Proc Natl Acad Sci U S A 107:16589-94. 2010
    ..The publicly available Pleiades MiniPromoter Project is a key resource to facilitate research on brain development and therapies...
  15. pmc Genome and transcriptome analyses of the mountain pine beetle-fungal symbiont Grosmannia clavigera, a lodgepole pine pathogen
    Scott DiGuistini
    Department of Wood Science, University of British Columbia, Vancouver, BC, Canada V6T 1Z4
    Proc Natl Acad Sci U S A 108:2504-9. 2011
    ..This work is a major step toward understanding the biological interactions between the tripartite MPB/fungus/forest system...
  16. pmc Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera
    Uljana Hesse-Orce
    Department of Wood Science, University of British Columbia, Vancouver, Canada
    BMC Genomics 11:536. 2010
    ..Trees respond to attacks from beetles and associated fungi by releasing terpenoid and phenolic defense compounds. It is unclear which genes are important for G. clavigera's ability to overcome antifungal pine terpenoids and phenolics...
  17. pmc Novel avian influenza H7N3 strain outbreak, British Columbia
    Martin Hirst
    British Columbia Cancer Agency BCCA Genome Sciences Centre, Vancouver, British Columbia, Canada
    Emerg Infect Dis 10:2192-5. 2004
    ..This insertion likely occurred by recombination between the hemagglutination and matrix genes in the LPAI virus...
  18. ncbi request reprint Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences
    Steven Ralph
    Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
    Mol Ecol 15:1275-97. 2006
    ..This study provides the first genome-scale approach to characterize insect-induced defences in a woody perennial providing a solid platform for functional investigation of plant-insect interactions in poplar...
  19. pmc Oligonucleotide microarray analysis of genomic imbalance in children with mental retardation
    J M Friedman
    Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada
    Am J Hum Genet 79:500-13. 2006
    ..This technology can detect at least twice as many potentially pathogenic de novo copy-number variants as conventional cytogenetic analysis can in people with mental retardation...
  20. pmc A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination
    Perseus I Missirlis
    Genome Sciences Centre, BC Cancer Agency, Suite 100, 570 West 7th Ave, Vancouver, BC, V5Z 4S6, Canada
    BMC Genomics 7:73. 2006
    ..In this study we introduce a high-throughput in vitro assay to rapidly detect novel loxP spacer mutants and describe the sequence characteristics of successful recombinants...
  21. pmc CAG-encoded polyglutamine length polymorphism in the human genome
    Stefanie L Butland
    UBC Bioinformatics Centre, Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
    BMC Genomics 8:126. 2007
    ....
  22. pmc Satellog: a database for the identification and prioritization of satellite repeats in disease association studies
    Perseus I Missirlis
    Genome Sciences Centre, BC Cancer Agency, Suite 100, 570 West 7th Ave, Vancouver, BC, V5Z 4S6, Canada
    BMC Bioinformatics 6:145. 2005
    ..Here we present Satellog, a database that catalogs all pure 1-16 repeat unit satellite repeats in the human genome along with supplementary data. Satellog analyzes each pure repeat in UniGene clusters for evidence of repeat polymorphism...
  23. doi request reprint Cytosolic protein interactions of the schizophrenia susceptibility gene dysbindin
    Carri Lyn R Mead
    Michael Smith Genome Sciences Centre, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada
    J Neurochem 113:1491-503. 2010
    ..Our results are consistent with the notion that impairment of aspects of the synaptic vesicle life cycle may be a pathogenic mechanism in schizophrenia...
  24. pmc Identification of a set of genes showing regionally enriched expression in the mouse brain
    Cletus A D'Souza
    Genome Sciences Centre, British Columbia Cancer Agency, 570 West 7th Ave Suite 100, Vancouver, BC, V5Z 4E6, Canada
    BMC Neurosci 9:66. 2008
    ....
  25. doi request reprint Clonal evolution of high-grade serous ovarian carcinoma from primary to recurrent disease
    Mauro Castellarin
    BC Cancer Agency, Michael Smith Genome Sciences Centre, 675 West 10th Avenue, Vancouver, BC, V5Z 1 L3, Canada
    J Pathol 229:515-24. 2013
    ..Moreover, they provide the first evidence at single nucleotide resolution that recurrent HGSC arises from multiple clones present in the primary tumour with negligible accumulation of new mutations during standard treatment...
  26. pmc High-throughput sequencing: a failure mode analysis
    George S Yang
    Canada s Michael Smith Genome Sciences Centre, BC Cancer Research Centre, Suite 100, 570 West 7th Avenue, Vancouver, BC, Canada
    BMC Genomics 6:2. 2005
    ..While high-throughput centres report failure rates typically on the order of 10%, the causes of sporadic sequencing failures are seldom analyzed in detail and have not, in the past, been formally reported...
  27. ncbi request reprint DNA copy-number analysis in bipolar disorder and schizophrenia reveals aberrations in genes involved in glutamate signaling
    Gary M Wilson
    Canada s Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Suite 100, 570 West 7th Avenue, Vancouver, BC, Canada V5Z 4S6
    Hum Mol Genet 15:743-9. 2006
    ..Further scrutiny of these regions may reveal insights into the etiology and genetic risk factors for these complex psychiatric disorders...
  28. pmc Identification by full-coverage array CGH of human DNA copy number increases relative to chimpanzee and gorilla
    Gary M Wilson
    Canada s Michael Smith Genome Sciences Centre, Vancouver, BC, Canada V5Z 4S6
    Genome Res 16:173-81. 2006
    ..The observation of increased copy number of a human cluster of core histone genes suggests that altered dosage, even of highly constrained genes, may be an important evolutionary mechanism...
  29. pmc Profiling the T-cell receptor beta-chain repertoire by massively parallel sequencing
    J Douglas Freeman
    Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada
    Genome Res 19:1817-24. 2009
    ..Direct sequence-based immunoprofiling will likely prove to be a useful tool for understanding repertoire dynamics in response to immune challenge, without a priori knowledge of antigen...
  30. pmc Systematic recovery and analysis of full-ORF human cDNA clones
    Agnes Baross
    Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, V5Z 4E6, Canada
    Genome Res 14:2083-92. 2004
    ..Clones containing novel splice variants or polymorphisms have also been identified. Our approach to clone recovery, applied at large scale, has the potential to recover many and possibly most of the genes absent from the MGC collection...
  31. pmc De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data
    Scott DiGuistini
    Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
    Genome Biol 10:R94. 2009
    ..Our results demonstrate that eukaryotic genome sequences can be accurately assembled by combining Illumina, 454 and Sanger sequence data...
  32. ncbi request reprint Assembling millions of short DNA sequences using SSAKE
    Rene L Warren
    British Columbia Cancer Agency, Genome Sciences Centre, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada
    Bioinformatics 23:500-1. 2007
    ..Availability: http://www.bcgsc.ca/bioinfo/software/ssake...
  33. doi request reprint BACE1 gene promoter single-nucleotide polymorphisms in Alzheimer's disease
    Weihui Zhou
    Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia, 2255 Wesbrook Mall, Vancouver, BC V6T1Z3, Canada
    J Mol Neurosci 42:127-33. 2010
    ..6 kb of the human BACE1 gene promoter region from late-onset AD patients and found that there was no significant association between single-nucleotide polymorphisms and AD cases...
  34. doi request reprint SP1 regulates a human SNAP-25 gene expression
    Fang Cai
    Department of Psychiatry, Brain Research Center, The University of British Columbia, Vancouver, British Columbia, Canada
    J Neurochem 105:512-23. 2008
    ..These results suggest that SP1 plays an important role in regulation of the human SNAP-25 gene expression...
  35. pmc Exhaustive T-cell repertoire sequencing of human peripheral blood samples reveals signatures of antigen selection and a directly measured repertoire size of at least 1 million clonotypes
    Rene L Warren
    BC Cancer Agency, Michael Smith Genome Sciences Centre, Vancouver, British Columbia V5Z 1L3, Canada
    Genome Res 21:790-7. 2011
    ..We also observed a highly statistically significant association between numbers of shared sequences and shared HLA class I alleles...
  36. pmc Complete haplotype sequence of the human immunoglobulin heavy-chain variable, diversity, and joining genes and characterization of allelic and copy-number variation
    Corey T Watson
    Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
    Am J Hum Genet 92:530-46. 2013
    ..3-0.5), with the greatest differences observed between African and Asian populations. These CNVs exhibit weak linkage disequilibrium with SNPs from two commercial arrays in most of the populations tested...
  37. pmc Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma
    Mauro Castellarin
    BC Cancer Agency, Michael Smith Genome Sciences Centre, Vancouver, British Columbia V5Z 1L3, Canada
    Genome Res 22:299-306. 2012
    ..5 × 10(-6)), and we observed a positive association with lymph node metastasis...
  38. pmc An integrated strategy to study muscle development and myofilament structure in Caenorhabditis elegans
    Barbara Meissner
    Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS Genet 5:e1000537. 2009
    ..Many of the genes affecting sarcomere integrity have human homologs for which little or nothing is known...
  39. doi request reprint Profiling model T-cell metagenomes with short reads
    Rene L Warren
    BC Cancer Agency, Michael Smith Genome Sciences Centre, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada
    Bioinformatics 25:458-64. 2009
    ..We developed a sequencing approach and assembly software (immuno-SSAKE or iSSAKE) for profiling T-cell metagenomes using short reads from the massively parallel sequencing platforms...
  40. pmc Analysis of long-lived C. elegans daf-2 mutants using serial analysis of gene expression
    Julius Halaschek-Wiener
    Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia V5Z 1L3, Canada
    Genome Res 15:603-15. 2005
    ..Comparison of our results to recent microarray studies highlights sets of overlapping genes that are highly conserved throughout evolution and thus represent strong candidate genes that control aging and longevity...
  41. doi request reprint Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice
    Jean Francois Schmouth
    Centre for Molecular Medicine and Therapeutics at the Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada
    BMC Biol 11:106. 2013
    ..In this study, we build upon our HuGX ('high-throughput human genes on the X chromosome') strategy to expand our understanding of human gene regulation in vivo...
  42. pmc Identification of novel androgen-responsive genes by sequencing of LongSAGE libraries
    Tammy L Romanuik
    Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    BMC Genomics 10:476. 2009
    ..The androgen pathway continues to be important in prostate cancer. Here, we evaluated the transcriptome of prostate cancer cells in response to androgen using long serial analysis of gene expression (LongSAGE) libraries...
  43. pmc The sensitivity of massively parallel sequencing for detecting candidate infectious agents associated with human tissue
    Richard A Moore
    Genome Sciences Centre, BC Cancer Agency, Vancouver, British Columbia, Canada
    PLoS ONE 6:e19838. 2011
    ..With current sequencing platforms approaching outputs of one billion reads per run, this is a highly sensitive method for detecting putative infectious agents associated with human tissues...
  44. ncbi request reprint A physical map of the highly heterozygous Populus genome: integration with the genome sequence and genetic map and analysis of haplotype variation
    Colin T Kelleher
    Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Plant J 50:1063-78. 2007
    ....
  45. doi request reprint A census of predicted mutational epitopes suitable for immunologic cancer control
    Rene L Warren
    British Columbia Cancer Agency, Genome Sciences Centre, Vancouver, British Columbia, Canada
    Hum Immunol 71:245-54. 2010
    ..From our data, we estimate that prophylactic vaccination could provide meaningful levels of prevention of tumors associated with common recurrent mutations...
  46. pmc Nonlinear electrophoretic response yields a unique parameter for separation of biomolecules
    Joel Pel
    Department of Physics and Astronomy, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
    Proc Natl Acad Sci U S A 106:14796-801. 2009
    ..Consequently, we are able to concentrate DNA while rejecting high concentrations of contaminants. We demonstrate one application of this method by extracting DNA from challenging samples originating in the Athabasca oil sands...
  47. pmc Simple, robust methods for high-throughput nanoliter-scale DNA sequencing
    Duane E Smailus
    Canada s Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada V5Z 4S6
    Genome Res 15:1447-50. 2005
    ..Implementation of these procedures at high-throughput genome centers could have a substantial impact on the amount of data that can be generated per unit cost...
  48. pmc Evolution of an adenocarcinoma in response to selection by targeted kinase inhibitors
    Steven Jm Jones
    Genome Sciences Centre, British Columbia Cancer Agency, 570 West 7th Avenue, Vancouver, BC, V5Z 4S6, Canada
    Genome Biol 11:R82. 2010
    ..We investigated the utility of massively parallel sequencing to characterize an adenocarcinoma of the tongue, before and after treatment...
  49. pmc A mouse atlas of gene expression: large-scale digital gene-expression profiles from precisely defined developing C57BL/6J mouse tissues and cells
    Asim S Siddiqui
    Canada s Michael Smith Genome Sciences Centre, British Columbia Cancer Research Centre, British Columbia Cancer Agency, Vancouver, BC, Canada V5Z 4S6
    Proc Natl Acad Sci U S A 102:18485-90. 2005
    ..The visual cortex, pancreas, mammary gland, preimplantation embryo, and placenta contain the largest number of differentially expressed transcripts, 25% of which are previously undescribed loci...
  50. ncbi request reprint The Genome sequence of the SARS-associated coronavirus
    Marco A Marra
    British Columbia Cancer Agency BCCA Genome Sciences Centre, 600 West 10th Avenue, Vancouver, British Columbia V5Z 4E6, Canada
    Science 300:1399-404. 2003
    ....
  51. pmc Targeted assembly of short sequence reads
    Rene L Warren
    Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    PLoS ONE 6:e19816. 2011
    ..Targeted assembly is a powerful method for interrogating large data sets for the presence of sequence variants of interest. TASR is a fast, flexible and easy to use tool for targeted assembly...
  52. ncbi request reprint A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome
    Richard J Mural
    Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA
    Science 296:1661-71. 2002
    ....
  53. ncbi request reprint The genome sequence of the malaria mosquito Anopheles gambiae
    Robert A Holt
    Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA
    Science 298:129-49. 2002
    ..An expressed sequence tag analysis of genes regulated by blood feeding provided insights into the physiological adaptations of a hematophagous insect...
  54. ncbi request reprint The Anopheles gambiae genome: an update
    Emmanuel Mongin
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Trends Parasitol 20:49-52. 2004
    ..This article reviews progress and considers limitations in the current sequence assembly and gene annotation, as well as approaches to address these problems and outstanding issues that users of the data must bear in mind...
  55. pmc The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)
    Daniela S Gerhard
    Genome Res 14:2121-7. 2004
    ..Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline...
  56. ncbi request reprint The ELT-2 GATA-factor and the global regulation of transcription in the C. elegans intestine
    James D McGhee
    Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    Dev Biol 302:627-45. 2007
    ..Other intestinal transcription factors would thus modulate the action of ELT-2, depending on the worm's nutritional and physiological needs...
  57. ncbi request reprint Duplication and divergence of 2 distinct pancreatic ribonuclease genes in leaf-eating African and Asian colobine monkeys
    John E Schienman
    Department of Biological Sciences, University at Albany, State University of New York, USA
    Mol Biol Evol 23:1465-79. 2006
    ..Conclusions relating our data to general hypotheses of evolution following gene duplication are discussed...
  58. pmc Sequencing and analysis of 10,967 full-length cDNA clones from Xenopus laevis and Xenopus tropicalis reveals post-tetraploidization transcriptome remodeling
    Ryan D Morin
    British Columbia Genome Sciences Centre, BCCA, Vancouver, BC V5Z 1L3 Canada
    Genome Res 16:796-803. 2006
    ..Approximately 14% of the paralogous pairs analyzed here also show differential expression indicative of subfunctionalization...
  59. ncbi request reprint Genome sequence of the Brown Norway rat yields insights into mammalian evolution
    Richard A Gibbs
    Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, MS BCM226, One Baylor Plaza, Houston, Texas 77030, USA lt http www hgsc bcm tmc edu
    Nature 428:493-521. 2004
    ....
  60. pmc The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron
    John F Etchberger
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA
    Genes Dev 21:1653-74. 2007
    ..Taken together, our results provide a unique view of the molecular landscape of a single neuron type and reveal an important aspect of the regulatory logic for gustatory neuron specification in C. elegans...
  61. ncbi request reprint Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster
    Evgeny M Zdobnov
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 298:149-59. 2002
    ....
  62. doi request reprint Synthetic genomes brought closer to life
    Robert A Holt
    Nat Biotechnol 26:296-7. 2008

Research Grants1