M Bouvier

Summary

Country: Canada

Publications

  1. pmc Impedance responses reveal β₂-adrenergic receptor signaling pluridimensionality and allow classification of ligands with distinct signaling profiles
    Wayne Stallaert
    Department of Biochemistry, Universite de Montreal, Montreal, Quebec, Canada
    PLoS ONE 7:e29420. 2012
  2. pmc Identification and characterization of an activating F229V substitution in the V2 vasopressin receptor in an infant with NSIAD
    Eric Carpentier
    Institute for Research in Immunology and Cancer, Universite de Montreal, C P 6128 Succursale Centre Ville, Montreal, Quebec, Canada
    J Am Soc Nephrol 23:1635-40. 2012
  3. pmc Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base
    Lucy Skrabanek
    Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, NY, USA
    BMC Bioinformatics 8:177. 2007
  4. ncbi Oligomerization of G-protein-coupled transmitter receptors
    M Bouvier
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Faculte de Medecine, Universite de Montreal, P O Box 6128, Down Town Station, Montreal, Quebec, H3C 3J7 Canada
    Nat Rev Neurosci 2:274-86. 2001
  5. ncbi When an inhibitor promotes activity
    Michel Bouvier
    Universite de Montreal, Institute for Research in Immunology and Cancer, Marcelle Coutu Pavillion, C P 6128 Succursale Centre Ville, Montreal, Quebec H3C 3J7, Canada
    Chem Biol 14:241-2. 2007
  6. ncbi Distinct regulation of beta 1- and beta 2-adrenergic receptors in Chinese hamster fibroblasts
    T Suzuki
    Department of Biochemistry, Universite de Montreal, Canada
    Mol Pharmacol 41:542-8. 1992
  7. ncbi Biochemical and biophysical demonstration of GPCR oligomerization in mammalian cells
    S Angers
    , , Quebec, Canada
    Life Sci 68:2243-50. 2001
  8. ncbi Homodimerization of the beta2-adrenergic receptor as a prerequisite for cell surface targeting
    Ali Salahpour
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 279:33390-7. 2004
  9. ncbi Agonist-modulated palmitoylation of beta 2-adrenergic receptor in Sf9 cells
    B Mouillac
    Department of Biochemistry, University of Montreal, Quebec, Canada
    J Biol Chem 267:21733-7. 1992
  10. ncbi Expression and recovery of functional G-protein-coupled receptors using baculovirus expression systems
    M Bouvier
    Department of Biochemistry, Universite de Montreal, QC, Canada
    Curr Opin Biotechnol 9:522-7. 1998

Collaborators

Detail Information

Publications93

  1. pmc Impedance responses reveal β₂-adrenergic receptor signaling pluridimensionality and allow classification of ligands with distinct signaling profiles
    Wayne Stallaert
    Department of Biochemistry, Universite de Montreal, Montreal, Quebec, Canada
    PLoS ONE 7:e29420. 2012
    ..Taken together, these data indicate that the pluridimensionality of GPCR signaling can be captured using integrative approaches to provide a comprehensive readout of drug activity...
  2. pmc Identification and characterization of an activating F229V substitution in the V2 vasopressin receptor in an infant with NSIAD
    Eric Carpentier
    Institute for Research in Immunology and Cancer, Universite de Montreal, C P 6128 Succursale Centre Ville, Montreal, Quebec, Canada
    J Am Soc Nephrol 23:1635-40. 2012
    ..These results emphasize the importance of genetic testing and the functional characterization of mutant receptors for patients with nephrogenic syndrome of inappropriate antidiuresis because the results might inform treatment decisions...
  3. pmc Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base
    Lucy Skrabanek
    Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, NY, USA
    BMC Bioinformatics 8:177. 2007
    ....
  4. ncbi Oligomerization of G-protein-coupled transmitter receptors
    M Bouvier
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Faculte de Medecine, Universite de Montreal, P O Box 6128, Down Town Station, Montreal, Quebec, H3C 3J7 Canada
    Nat Rev Neurosci 2:274-86. 2001
    ..Here, we review the evidence supporting the existence of G-protein-coupled-receptor dimerization and discuss its functional importance...
  5. ncbi When an inhibitor promotes activity
    Michel Bouvier
    Universite de Montreal, Institute for Research in Immunology and Cancer, Marcelle Coutu Pavillion, C P 6128 Succursale Centre Ville, Montreal, Quebec H3C 3J7, Canada
    Chem Biol 14:241-2. 2007
  6. ncbi Distinct regulation of beta 1- and beta 2-adrenergic receptors in Chinese hamster fibroblasts
    T Suzuki
    Department of Biochemistry, Universite de Montreal, Canada
    Mol Pharmacol 41:542-8. 1992
    ..In fact, both agonist-induced sequestration and down-regulation are considerably blunted for the beta 1AR, compared with the beta 2AR...
  7. ncbi Biochemical and biophysical demonstration of GPCR oligomerization in mammalian cells
    S Angers
    , , Quebec, Canada
    Life Sci 68:2243-50. 2001
    ..Our results clearly demonstrate the presence of constitutive beta2AR oligomers in living cells that can be modulated by the selective adrenergic agonist isoproterenol, suggesting a pertinent physiological role for GPCR oligomerization...
  8. ncbi Homodimerization of the beta2-adrenergic receptor as a prerequisite for cell surface targeting
    Ali Salahpour
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 279:33390-7. 2004
    ..Taken together, these data indicate that beta2AR homodimerization plays an important role in ER export and cell surface targeting...
  9. ncbi Agonist-modulated palmitoylation of beta 2-adrenergic receptor in Sf9 cells
    B Mouillac
    Department of Biochemistry, University of Montreal, Quebec, Canada
    J Biol Chem 267:21733-7. 1992
    ..These results suggest that agonist-mediated regulation of beta 2-AR post-translational palmitoylation could represent an other regulatory process for G protein-coupled receptors...
  10. ncbi Expression and recovery of functional G-protein-coupled receptors using baculovirus expression systems
    M Bouvier
    Department of Biochemistry, Universite de Montreal, QC, Canada
    Curr Opin Biotechnol 9:522-7. 1998
    ..Methods that allow easy and selective recovery of properly folded and mature receptors in viral particles open new perspectives for such applications...
  11. ncbi Newly synthesized human delta opioid receptors retained in the endoplasmic reticulum are retrotranslocated to the cytosol, deglycosylated, ubiquitinated, and degraded by the proteasome
    U E Petaja-Repo
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 276:4416-23. 2001
    ....
  12. ncbi Bioluminescence resonance energy transfer reveals ligand-induced conformational changes in CXCR4 homo- and heterodimers
    Yann Percherancier
    Department of Biochemistry, Universite de Montreal, Montreal H3C 3J7, Quebec, Canada
    J Biol Chem 280:9895-903. 2005
    ....
  13. ncbi Dimerization: an emerging concept for G protein-coupled receptor ontogeny and function
    Stephane Angers
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, H3C 3J7, Canada
    Annu Rev Pharmacol Toxicol 42:409-35. 2002
    ..In addition to fundamentally changing our views on the structure and activation processes of GPCRs, the concept of homo- and heterodimerization could have dramatic impacts on drug development and screening...
  14. ncbi Reciprocal regulation of agonist and inverse agonist signaling efficacy upon short-term treatment of the human delta-opioid receptor with an inverse agonist
    Graciela Pineyro
    Centre de Recherche Fernand Seguin, 7331 rue Hochelaga, Montreal, Quebec, H1N 3V2 Canada
    Mol Pharmacol 67:336-48. 2005
    ..Taken together, these data show that inverse agonists may induce rapid regulation in receptor signaling efficacy...
  15. doi VRQ397 (CRAVKY): a novel noncompetitive V2 receptor antagonist
    L Rihakova
    Departments of Pediatrics and Pharmacology, Hopital Ste Justine, Research Center, Montreal, QC, Canada
    Am J Physiol Regul Integr Comp Physiol 297:R1009-18. 2009
    ..We hereby describe the discovery of a first potent noncompetitive antagonist of V2R, which exhibits functional selectivity, in line with properties of a negative allosteric modulator...
  16. ncbi Hetero-oligomerization between beta2- and beta3-adrenergic receptors generates a beta-adrenergic signaling unit with distinct functional properties
    Andreas Breit
    Département de Biochimie et Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 279:28756-65. 2004
    ..Together, these results demonstrate that hetero-oligomerization between beta(2)AR and beta(3)AR forms a beta-adrenergic signaling unit that possesses unique functional properties...
  17. ncbi Src-dependent phosphorylation of beta2-adaptin dissociates the beta-arrestin-AP-2 complex
    Delphine Fessart
    Hormones and Cancer Research Unit, Department of Medicine, Royal Victoria Hospital, Montreal, QC, H3A 1A1, Canada
    J Cell Sci 120:1723-32. 2007
    ..Our findings not only unveil beta2-adaptin as a new Src target during AT1R internalization, but also support the role of receptor-mediated signaling in the control of clathrin-dependent endocytosis of receptors...
  18. ncbi Ca(2+)-dependent sensitization of adenylyl cyclase activity
    G Rousseau
    , , Montreal, Quebec, Canada
    Eur J Pharmacol 422:53-60. 2001
    ..Taken together, our data reveal the existence of a CamKinase-dependent sensitization process acting at the level of the adenylyl cyclase catalytic moiety...
  19. ncbi Structural and functional aspects of G protein-coupled receptor oligomerization
    T E Hebert
    Centre de Recherche, Institut de Cardiologie de Montreal, Universite de Montreal, QC, Canada
    Biochem Cell Biol 76:1-11. 1998
    ..In this paper, we review the biochemical and functional evidence supporting this notion...
  20. ncbi Allosteric effects of G protein overexpression on the binding of beta-adrenergic ligands with distinct inverse efficacies
    M Azzi
    , , , , Canada
    Mol Pharmacol 60:999-1007. 2001
    ..Taken together, these results demonstrate that the apparent receptor number detected by radiolabeled inverse agonists is affected by the content in G proteins as a result of their influence on R/R* isomerization...
  21. ncbi Protein-protein interaction and not glycosylation determines the binding selectivity of heterodimers between the calcitonin receptor-like receptor and the receptor activity-modifying proteins
    S Hilairet
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 276:29575-81. 2001
    ..Overall, the data indicate that direct protein-protein interactions dictate the pharmacological properties of the CRLR-RAMP complexes...
  22. ncbi Export from the endoplasmic reticulum represents the limiting step in the maturation and cell surface expression of the human delta opioid receptor
    U E Petaja-Repo
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 275:13727-36. 2000
    ..Whether or not the low efficiency of the ER export is a general feature among G protein-coupled receptors remains to be investigated...
  23. ncbi Palmitoylation of the V2 vasopressin receptor carboxyl tail enhances beta-arrestin recruitment leading to efficient receptor endocytosis and ERK1/2 activation
    Pascale G Charest
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 278:41541-51. 2003
    ..Taken together the results indicate that palmitoylation enhances the recruitment of beta-arrestin to the activated V2 vasopressin receptor thus facilitating processes requiring the scaffolding action of beta-arrestin...
  24. ncbi The gene product of the gp78/AMFR ubiquitin E3 ligase cDNA is selectively recognized by the 3F3A antibody within a subdomain of the endoplasmic reticulum
    Marilyn Registre
    Departement de pathologie et biologie cellulaire, Universite de Montreal, Que, Canada
    Biochem Biophys Res Commun 320:1316-22. 2004
    ..The3F3A anti-AMFR mAb therefore selectively recognizes a subpopulation of expressed AMFR localized to a subdomain of the ER...
  25. pmc Functional rescue of beta-adrenoceptor dimerization and trafficking by pharmacological chaperones
    Hiroyuki Kobayashi
    Departement de Biochimie, Groupe de Recherche Universitaire sur le Médicament and Institut de recherche en Immunologie et en Cancérologie, Universite de Montreal, Quebec, Canada
    Traffic 10:1019-33. 2009
    ....
  26. ncbi Conformational rearrangements and signaling cascades involved in ligand-biased mitogen-activated protein kinase signaling through the beta1-adrenergic receptor
    Ségolène Galandrin
    Department of Biochemistry and Groupe de Recherche Universitaire sur le Médicament, Institute for Research in Immunology and Cancer, Universite de Montreal, Montreal, Quebec, Canada
    Mol Pharmacol 74:162-72. 2008
    ....
  27. pmc Receptor activity-independent recruitment of betaarrestin2 reveals specific signalling modes
    Sonia Terrillon
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Quebec, Canada
    EMBO J 23:3950-61. 2004
    ..These data demonstrate that betaarrestin2 can act as a 'bonafide' signalling molecule even in the absence of activated receptor...
  28. ncbi Functional calcitonin gene-related peptide receptors are formed by the asymmetric assembly of a calcitonin receptor-like receptor homo-oligomer and a monomer of receptor activity-modifying protein-1
    Madeleine Heroux
    Department of Biochemistry and Groupe de Recherche Universitaire sur le Médicament, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 282:31610-20. 2007
    ....
  29. pmc Coordinated action of NSF and PKC regulates GABAB receptor signaling efficacy
    Stephanie M Pontier
    Département de Biochimie and Groupe de Recherche Universitaire sur le Médicament, Institut de Recherche en Immunologie et Cancérologie, Universite de Montreal, Montreal, QC, Canada
    EMBO J 25:2698-709. 2006
    ..The functional interaction between three bona fide regulators of neurotransmitter release, such as GBR, NSF and PKC, could shed new light on the modulation of presynaptic GBR action...
  30. ncbi Unraveling G protein-coupled receptor endocytosis pathways using real-time monitoring of agonist-promoted interaction between beta-arrestins and AP-2
    Fadi F Hamdan
    Department of Biochemistry, Groupe de Recherche Universitaire sur le Médicament, Institute for Research in Immunology and Cancer, Universite de Montreal, Montreal, Quebec, Canada
    J Biol Chem 282:29089-100. 2007
    ....
  31. ncbi Oligomerization of transcriptional intermediary factor 1 regulators and interaction with ZNF74 nuclear matrix protein revealed by bioluminescence resonance energy transfer in living cells
    Delphine Germain-Desprez
    Department of Biochemistry, Universite de Montreal, C P 6128, Succ Centre Ville, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 278:22367-73. 2003
    ....
  32. ncbi Beta 1/beta 2-adrenergic receptor heterodimerization regulates beta 2-adrenergic receptor internalization and ERK signaling efficacy
    Catherine Lavoie
    Centre de Recherche, Institut de Cardiologie de Montreal, Montreal, Quebec, H1T 1C8, Canada
    J Biol Chem 277:35402-10. 2002
    ..Taken together, our data suggest that heterodimerization between beta(1)AR and beta(2)AR inhibits the agonist-promoted internalization of the beta(2)AR and its ability to activate the ERK1/2 MAPK signaling pathway...
  33. ncbi Role of palmitoylation/depalmitoylation reactions in G-protein-coupled receptor function
    Riad Qanbar
    Departement de Biochimie, Universite de Montreal, C P 6128 Succursale Centre Ville, 2900 Edouard Montpetit, Montreal, Quebec, Canada H3C 3J7
    Pharmacol Ther 97:1-33. 2003
    ..The palmitoylation of other proteins involved in GPCR signaling, such as G-proteins, regulators of G-protein signaling, and G-protein-coupled receptor kinases, is also discussed...
  34. ncbi Functional desensitization to isoproterenol without reducing cAMP production in canine failing cardiocytes
    C E Laurent
    , , , , Canada
    Am J Physiol Regul Integr Comp Physiol 280:R355-64. 2001
    ....
  35. ncbi The human beta 3-adrenergic receptor is resistant to short term agonist-promoted desensitization
    F Nantel
    Departement de Biochimie, Universite de Montreal, Quebec, Canada
    Mol Pharmacol 43:548-55. 1993
    ....
  36. ncbi Association of calnexin with wild type and mutant AVPR2 that causes nephrogenic diabetes insipidus
    J P Morello
    , , , Quebec, Canada
    Biochemistry 40:6766-75. 2001
    ....
  37. pmc Ligands act as pharmacological chaperones and increase the efficiency of delta opioid receptor maturation
    Ulla E Petäjä-Repo
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    EMBO J 21:1628-37. 2002
    ..This novel property of G protein-coupled receptor ligands may have important implications when considering their effects on cellular responsiveness during therapeutic treatments...
  38. ncbi Real-time monitoring of receptor and G-protein interactions in living cells
    Céline Gales
    Department of Biochemistry, Universite de Montreal, P O Box 6128 Down town station, Montreal, H3C 3J7, Canada
    Nat Methods 2:177-84. 2005
    ....
  39. ncbi Real-time monitoring of ubiquitination in living cells by BRET
    Julie Perroy
    Département de Biochimie and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, C P 6128 Succursale Centre Ville Montréal, Quebec H3C 3J7, Canada
    Nat Methods 1:203-8. 2004
    ..The ubiquitination BRET assay should prove to be a useful tool for studying the dynamic ubiquitination of proteins and for understanding which cellular functions are regulated by this post-translational event...
  40. ncbi Quantitative assessment of beta 1- and beta 2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer
    Jean Francois Mercier
    Département de Biochimie and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 277:44925-31. 2002
    ....
  41. ncbi The V2 vasopressin receptor stimulates ERK1/2 activity independently of heterotrimeric G protein signalling
    Pascale G Charest
    Department of Biochemistry and Groupe de Recherche Universitaire sur le Médicament, Institute for Research in Immunology and Cancer, Universite de Montreal, C P 6128 Succursale Centre Ville, Montreal Quebec Canada H3C 3J7
    Cell Signal 19:32-41. 2007
    ..These findings demonstrate that beta arrestin is a genuine signalling initiator that can, on its own, engage a MAPK activation machinery upon stimulation of a GPCR by its natural ligand...
  42. ncbi Assembly and signaling of CRLR and RAMP1 complexes assessed by BRET
    Madeleine Heroux
    Departement de Biochimie, Institut de Recherche en Immunologie et Cancérologie and Groupe de Recherche Universitaire sur le Médicament, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal, QC, Canada H3C 3J7
    Biochemistry 46:7022-33. 2007
    ..In addition to shed new light on the CRLR-RAMP1 signaling complex, the BRET assays developed herein offer new biosensors for probing CGRP receptor activity...
  43. ncbi High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based beta-arrestin2 recruitment assay
    Fadi F Hamdan
    University of Montreal, Department of Biochemistry, Montreal, Quebec, Canada
    J Biomol Screen 10:463-75. 2005
    ..This is the 1st study describing a BRET1-beta-arrestin recruitment assay in stable mammalian cells and its successful application in HTS for GPCRs antagonists...
  44. ncbi Distinct signaling profiles of beta1 and beta2 adrenergic receptor ligands toward adenylyl cyclase and mitogen-activated protein kinase reveals the pluridimensionality of efficacy
    Ségolène Galandrin
    Department of Biochemistry, Groupe de Recherche Universitaire sur le Médicament and Institute for Research in Immunology and Cancer, Universite de Montreal, C P 6128 Succursale Centre Ville, Montreal, QC, Canada, H3C 3J7
    Mol Pharmacol 70:1575-84. 2006
    ..This should have important implications for the future design of screening assays used in drug discovery campaigns...
  45. ncbi An aplysia dopamine1-like receptor: molecular and functional characterization
    Demian Barbas
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec, Canada
    J Neurochem 96:414-27. 2006
    ..Apdop1 sensitivity to dopamine makes it a potential modulator of operant conditioning procedure...
  46. pmc Functional characterization of vasopressin type 2 receptor substitutions (R137H/C/L) leading to nephrogenic diabetes insipidus and nephrogenic syndrome of inappropriate antidiuresis: implications for treatments
    Moulay D Rochdi
    Institut de Recherche en Immunologie et Cancérologie, Departement de Biochimie, Universite de Montreal, Montreal, Quebec, Canada
    Mol Pharmacol 77:836-45. 2010
    ....
  47. pmc Beta-arrestin-mediated activation of MAPK by inverse agonists reveals distinct active conformations for G protein-coupled receptors
    Mounia Azzi
    Department of Biochemistry, Universite de Montreal, Montreal, QC, Canada H3C 3J7
    Proc Natl Acad Sci U S A 100:11406-11. 2003
    ..These results point to a multistate model of receptor activation in which ligand-specific conformations are capable of differentially activating distinct signaling partners...
  48. doi Protein-protein interactions monitored in cells from transgenic mice using bioluminescence resonance energy transfer
    Martin Audet
    Department of Biochemistry, Institute for Research in Immunology and Cancer, and Groupe de Recherche Universitaire sur le Médicament, Universite de Montreal, Montreal, Quebec, Canada
    FASEB J 24:2829-38. 2010
    ..Our study demonstrates that BRET can be used to monitor the dynamic regulation of protein-protein interactions in cells derived from transgenic mice...
  49. ncbi The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS)
    Lucie Bertrand
    BioSignal Packard Inc, 1744 William, Montreal, Quebec, H3J IR4, Canada
    J Recept Signal Transduct Res 22:533-41. 2002
    ....
  50. ncbi Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin
    S Hilairet
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec, H3C 3J7, Canada
    J Biol Chem 276:42182-90. 2001
    ..These results therefore indicate that although atypical by its heterodimeric composition and its targeting to the plasma membrane, the CGRP receptor shares endocytotic mechanisms that are common to most classical 7TM receptors...
  51. ncbi Short-term inverse-agonist treatment induces reciprocal changes in delta-opioid agonist and inverse-agonist binding capacity
    G Pineyro
    Departement de Biochimie, Universite de Montreal, Montreal, Canada
    Mol Pharmacol 60:816-27. 2001
    ....
  52. ncbi Increased production of active human beta(2)-adrenergic/G(alphas) fusion receptor in Sf-9 cells using nutrient limiting conditions
    E Carpentier
    Bioprocess Sector, Biotechnology Research Institute, 6100 Royalmount Avenue, Montreal, Quebec, H4P 2R2, Canada
    Protein Expr Purif 23:66-74. 2001
    ....
  53. ncbi Primary sequence requirements for S-acylation of beta(2)-adrenergic receptor peptides
    C Belanger
    Département de Biochimie and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, C P 6128, Succursale Centre Ville, H3C 3J7, Montreal, QC, Canada
    FEBS Lett 499:59-64. 2001
    ..Whether or not these sequence characteristics also contribute to the selectivity of palmitoylation in vivo will need to be further investigated...
  54. doi Combining resonance energy transfer methods reveals a complex between the alpha2A-adrenergic receptor, Galphai1beta1gamma2, and GRK2
    Billy Breton
    Department of Biochemistry, Institute for Research in Immunology and Cancer, and Groupe de Recherche Universitaire sur le Médicament, Universite de Montreal, Montreal, Quebec, Canada
    FASEB J 24:4733-43. 2010
    ..RET combination analysis revealed unanticipated dynamics in GPCR signaling and will be applicable to many biological systems...
  55. pmc Functional effects of long-term activation on human beta 2- and beta 3-adrenoceptor signalling
    F Nantel
    , , , Canada
    Br J Pharmacol 114:1045-51. 1995
    ..These findings might have practical implications for future strategies involving long-term therapies with receptor agonists...
  56. pmc Pharmacological chaperones rescue cell-surface expression and function of misfolded V2 vasopressin receptor mutants
    J P Morello
    Département de Biochimie and Le Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Clin Invest 105:887-95. 2000
    ....
  57. doi Monitoring protein-protein interactions in living cells by bioluminescence resonance energy transfer (BRET)
    Fadi F Hamdan
    University of Montreal, Montreal, Quebec, Canada
    Curr Protoc Neurosci . 2006
    ..This noninvasive and homogeneous assay provides a robust and sensitive proteomic platform with applications for basic science research and drug discovery...
  58. ncbi Insights into signaling from the beta2-adrenergic receptor structure
    Martin Audet
    Department of Biochemistry, Institute for Research in Immunology and Cancer, Groupe de Recherche Universitaire sur le Médicament, Universite de Montreal, CP 6128 Succursale Centre Ville, Montreal, Quebec H3C 3J7, Canada
    Nat Chem Biol 4:397-403. 2008
    ..The different binding modes of these ligands, which correlated with their reported efficacy profiles, suggest that it could be possible to predict the structural determinants of drug signaling efficacies...
  59. pmc Bioluminescence resonance energy transfer assays reveal ligand-specific conformational changes within preformed signaling complexes containing delta-opioid receptors and heterotrimeric G proteins
    Nicolas Audet
    Centre de Recherche Fernand Seguin, Hopital Louis H Lafontaine, Montreal, Quebec, Canada
    J Biol Chem 283:15078-88. 2008
    ..The existence of multiple active receptor states has implications for the way we conceive specificity of signal transduction...
  60. ncbi Calcitonin gene-related peptide analogues with aza and indolizidinone amino acid residues reveal conformational requirements for antagonist activity at the human calcitonin gene-related peptide 1 receptor
    Damien Boeglin
    Departement de Chimie, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal, Quebec, Canada H3C 3J7
    J Med Chem 50:1401-8. 2007
    ..The structure-activity relationships exhibited by our 26 analogues illustrate conformational requirements important for designing CGRP antagonists and highlight the importance of beta-turns centered at Gly33-Pro34 for potency...
  61. ncbi Probing the activation-promoted structural rearrangements in preassembled receptor-G protein complexes
    Céline Gales
    Department of Biochemistry and Groupe de Recherche Universitaire sur le Médicament, Institute for Research in Immunology and Cancer, Universite de Montreal, P O Box 6128, Downtown Station, Montreal, Quebec, Canada H3C 3J7
    Nat Struct Mol Biol 13:778-86. 2006
    ..This conformational change may represent the movement required to allow nucleotide exit from the G alpha subunit, thus reflecting the initial activation event...
  62. pmc Multimerization of Staufen1 in live cells
    Catherine Martel
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    RNA 16:585-97. 2010
    ..Altogether, these results indicate that Stau1 self-associates in mRNPs via its multiple functional domains that can select mRNAs to be transported and establish protein-protein interaction...
  63. pmc Cholesterol-dependent separation of the beta2-adrenergic receptor from its partners determines signaling efficacy: insight into nanoscale organization of signal transduction
    Stephanie M Pontier
    Institut de Recherche en Immunologie et Cancérologie, Departement de Biochimie, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 283:24659-72. 2008
    ....
  64. ncbi Pharmacological chaperone action on G-protein-coupled receptors
    Virginie Bernier
    Département de Biochimie and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, Montreal, Quebec, Canada H3C 3J7
    Curr Opin Pharmacol 4:528-33. 2004
    ..Although only a few examples are currently available, the observation that pharmacological chaperones can also favour the folding of wild-type G-protein-coupled receptors indicates that these compounds could have wide applications...
  65. ncbi Pharmacological chaperones: potential treatment for conformational diseases
    Virginie Bernier
    Département de Biochimie and Le Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, H3T 1J4, Canada
    Trends Endocrinol Metab 15:222-8. 2004
    ....
  66. ncbi Characterization of oligomeric human ATP binding cassette transporter A1. Potential implications for determining the structure of nascent high density lipoprotein particles
    Maxime Denis
    Cardiovascular Genetics Laboratory, Cardiology Division, McGill University Health Centre Royal Victoria Hospital, Montreal, Quebec H3A 1A1, Canada
    J Biol Chem 279:41529-36. 2004
    ....
  67. ncbi Determination of protein-bound palmitate turnover rates using a three-compartment model that formally incorporates [3H]palmitate recycling
    Riad Qanbar
    Department of Biochemistry, Universite de Montreal, Montreal, QC, Canada H3C 3J7
    Biochemistry 43:12275-88. 2004
    ..The model may also be generally applicable to other areas of research where recycling of tracer is a concern...
  68. ncbi Functional rescue of the constitutively internalized V2 vasopressin receptor mutant R137H by the pharmacological chaperone action of SR49059
    Virginie Bernier
    Department of Biochemistry, Universite de Montreal, C P 6128 Succursale Centre Ville, Montreal, Quebec, Canada H3C 3J7
    Mol Endocrinol 18:2074-84. 2004
    ..SR49059 treatment significantly improved its maturation and cell surface targeting, indicating that the functional rescue of R137H V2Rs results from the pharmacological chaperone action of the antagonist...
  69. pmc Heterodimerization of V1a and V2 vasopressin receptors determines the interaction with beta-arrestin and their trafficking patterns
    Sonia Terrillon
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec, Canada H3C 3J7
    Proc Natl Acad Sci U S A 101:1548-53. 2004
    ....
  70. pmc Phosphorylation-independent desensitization of GABA(B) receptor by GRK4
    Julie Perroy
    Departement de Biochimie, Universite de Montreal, C P 6128 Succursale Centre Ville, Montreal, Quebec, Canada
    EMBO J 22:3816-24. 2003
    ....
  71. pmc Roles of G-protein-coupled receptor dimerization
    Sonia Terrillon
    Department of Biochemistry, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal, Quebec, Canada H3C 3J7
    EMBO Rep 5:30-4. 2004
    ..In particular, the changes in ligand-binding and signalling properties that accompany heterodimerization could give rise to an unexpected pharmacological diversity that would need to be considered...
  72. ncbi Biochemical characterization of beta2-adrenergic receptor dimers and oligomers
    Ali Salahpour
    Department of Biochemistry and Groupe de Recherche sur le Système Nerveux Autonome, Universite de Montreal, 2900 Edouard Montpetit, P O Box 6128, Succ Centre Ville, Montreal H3C 3J7, Canada
    Biol Chem 384:117-23. 2003
    ....
  73. ncbi Monitoring of ligand-independent dimerization and ligand-induced conformational changes of melatonin receptors in living cells by bioluminescence resonance energy transfer
    Mohammed A Ayoub
    Department of Cell Biology, Institut Cochin, INSERM U 567, CNRS UMR 8104, 22 rue Mechain, 75014 Paris, France
    J Biol Chem 277:21522-8. 2002
    ..The observation that ligands favored the energy transfer within the hetero-oligomer from MT1R to MT2R but not in the reverse orientation, from MT2R to MT1R, supports this view...
  74. ncbi Heterotrimeric G proteins form stable complexes with adenylyl cyclase and Kir3.1 channels in living cells
    R Victor Rebois
    Laboratory of Cellular Biology, 5 Research Court, National Institute of Deafness and Communicative Disorders, National Institutes of Health, Rockville, MD 20850, USA
    J Cell Sci 119:2807-18. 2006
    ..Furthermore, receptor-sensitive G-protein-effector complexes could be detected before being transported to the plasma membrane, providing the first direct evidence for an intracellular site of assembly...
  75. ncbi Adenosine A2A-dopamine D2 receptor-receptor heteromerization: qualitative and quantitative assessment by fluorescence and bioluminescence energy transfer
    Meritxell Canals
    Department of Biochemistry and Molecular Biology, University of Barcelona, Martí y Franquès 1, E 08028 Barcelona, Spain
    J Biol Chem 278:46741-9. 2003
    ..In the most probable one, helix 5 and/or helix 6 and the N-terminal portion of I3 from D2R approached helix 4 and the C-terminal portion of the C-tail from the A2AR, respectively...
  76. ncbi Distinct motifs of neuropeptide Y receptors differentially regulate trafficking and desensitization
    Moussa Ouedraogo
    Institut Gilbert Laustriat, UMR 7175, CNRS Université Louis Pasteur, Strasbourg I, France
    Traffic 9:305-24. 2008
    ..Overall, our results indicate that beta-arrestin-mediated desensitization and internalization of Y(1) and Y(2) receptors are differentially regulated by the C-terminal motif and the i2 loop consensus motif...
  77. ncbi Constitutive agonist-independent CCR5 oligomerization and antibody-mediated clustering occurring at physiological levels of receptors
    Hassan Issafras
    Department of Cell Biology, Cochin Institute, INSERM U 567, CNRS UMR 8104 and University René Descartes, Paris 75014, France
    J Biol Chem 277:34666-73. 2002
    ..Taken together, our data indicate that constitutive receptor homo-oligomerization has a biologically relevant significance and might be involved in the process of receptor biosynthesis...
  78. ncbi A cyclic peptide mimicking the third intracellular loop of the V2 vasopressin receptor inhibits signaling through its interaction with receptor dimer and G protein
    Sebastien Granier
    Unité INSERM U469, CCIPE, 141 rue de la Cardonille, 34094 Montpellier, France
    J Biol Chem 279:50904-14. 2004
    ..Taken together, the data demonstrate that a peptide mimicking V2-R third intracellular loop affects both the dimeric structural organization of the receptor and has direct inhibitory action on Gs...
  79. ncbi Oxytocin and vasopressin V1a and V2 receptors form constitutive homo- and heterodimers during biosynthesis
    Sonia Terrillon
    Institut National de la Sante et de la Recherche Medicale, Cedex 5, France
    Mol Endocrinol 17:677-91. 2003
    ..Taken together, these results strongly support the notion that GPCR dimerization is a constitutive process...
  80. pmc Glycoprotein hormone receptors: link between receptor homodimerization and negative cooperativity
    Eneko Urizar
    IRIBHM, Universite Libre de Bruxelles, Campus Erasme, Brussels, Belgium
    EMBO J 24:1954-64. 2005
    ..This study is the first report of homodimerization associated with negative cooperativity in rhodopsin-like GPCRs. As such, it may warrant revisitation of allosterism in the whole GPCR family...
  81. ncbi [New talk between receptor and trimeric G proteins: "an intertwined body dance"]
    Céline Gales
    INSERM U858, Equipe 8 Pharmacologie moléculaire et clinique du système nerveux autonome, I2MR, CHU Rangueil, BP84225, 31432 Toulouse Cedex 4, France
    Med Sci (Paris) 23:1031-4. 2007
  82. ncbi G protein-coupled receptors form stable complexes with inwardly rectifying potassium channels and adenylyl cyclase
    Natalie Lavine
    Centre for Addiction and Mental Health, Department of Pharmacology, Institute of Medical Science, University of Toronto, Ontario M5T 1R8, Canada
    J Biol Chem 277:46010-9. 2002
    ..The observation that several G protein-coupled receptors form stable complexes with their effectors suggests that this arrangement might be a general feature of G protein-coupled signal transduction...
  83. ncbi Homodimerization of adenosine A2A receptors: qualitative and quantitative assessment by fluorescence and bioluminescence energy transfer
    Meritxell Canals
    Department of Biochemistry and Molecular Biology of the University of Barcelona, Barcelona, Spain
    J Neurochem 88:726-34. 2004
    ..As A2AR and dopamine D2 receptors do indeed interact, these results indicate that A2AR homodimers are the functional species at the cell surface and that they coexist with A2AR/D2 receptor heterodimers...
  84. ncbi Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type Frizzled in the endoplasmic reticulum by oligomerization
    Ajamete Kaykas
    Howard Hughes Medical Institute, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Nat Cell Biol 6:52-8. 2004
    ..These data support the hypothesis that oligomerization of mutant and wild-type Fz proteins occurs in the endoplasmic reticulum and may explain the genetic dominance of this FEVR allele...
  85. ncbi Functional characterization of a novel serotonin receptor (5-HTap2) expressed in the CNS of Aplysia californica
    Demian Barbas
    Department de Biochimie, Universite de Montreal, Quebec, Canada
    J Neurochem 80:335-45. 2002
    ..Moreover, the high expression of 5-HTap2 in the bag cells, associated with its pharmacological profile, suggests that this receptor may be implicated in modulating the afterdischarge during the egg-laying behavior...
  86. ncbi Resonance energy transfer approaches in molecular pharmacology and beyond
    Stefano Marullo
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris F 75014, France
    Trends Pharmacol Sci 28:362-5. 2007
    ..In this article, we summarize the principal trends of the field that were discussed during the workshop. Consensus guidelines for obtaining reliable information from RET-based experiments are also presented...
  87. ncbi Emerging role of homo- and heterodimerization in G-protein-coupled receptor biosynthesis and maturation
    Sébastien Bulenger
    Cell Biology Department, Institut Cochin, 27 rue du Fg St Jacques, 75014 Paris, France
    Trends Pharmacol Sci 26:131-7. 2005
    ..However, increasing evidence suggests that homodimerization might have a similar role in the receptor maturation process for many GPCRs...
  88. ncbi A human immunodeficiency virus type 1 protease biosensor assay using bioluminescence resonance energy transfer
    Kimberly Hu
    HIV 1 RNA Trafficking Laboratory, Sir Mortimer B Davis Jewish General Hospital, 3999 Cote Ste Catherine Road, Montreal, Que, Canada H3T 1E2
    J Virol Methods 128:93-103. 2005
    ..This PR BRET2 biosensor assay can be adapted for high throughput screening of new HIV-1 PR inhibitors. It can be employed to screen for antiviral compounds that also target the proteases of other viruses...
  89. ncbi Methods to monitor the quaternary structure of G protein-coupled receptors
    Graeme Milligan
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, UK
    FEBS J 272:2914-25. 2005
    ..Herein we assess the widely applied techniques and discuss the relative benefits and limitations of these approaches...
  90. pmc BRET analysis of GPCR oligomerization: newer does not mean better
    Michel Bouvier
    Nat Methods 4:3-4; author reply 4. 2007
  91. ncbi Heterodimerization of beta1- and beta2-adrenergic receptor subtypes optimizes beta-adrenergic modulation of cardiac contractility
    Wei Zhong Zhu
    Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224, USA
    Circ Res 97:244-51. 2005
    ....
  92. ncbi Homo- and hetero-oligomerization of beta-arrestins in living cells
    Hélène Storez
    Department of Cell Biology, Institut Cochin, Paris, F 75014 France
    J Biol Chem 280:40210-5. 2005
    ..Our data clearly indicate that beta-arrestins can exist as homo- and hetero-oligomers in living cells and raise the hypothesis that the oligomeric state may regulate their subcellular distribution and functions...
  93. ncbi International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the recognition and nomenclature of G protein-coupled receptor heteromultimers
    Jean Philippe Pin
    Institut de Genomique Fonctionnelle, 141, rue de la Cardonille, 34094 Montpellier Cedex 5, France
    Pharmacol Rev 59:5-13. 2007
    ....