Guy Boivin

Summary

Country: Canada

Publications

  1. ncbi Expression of the late cytomegalovirus (CMV) pp150 transcript in leukocytes of AIDS patients is associated with a high viral DNA load in leukocytes and presence of CMV DNA in plasma
    G Boivin
    Division of Microbiology and Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Sainte Foy, Quebec, Canada G1V 4G2
    J Infect Dis 179:1101-7. 1999
  2. pmc Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses
    Guy Boivin
    Research Center in Infectious Diseases of the CHUQ CHUL, Department of Pediatrics, Laval University, Quebec City, Canada
    J Clin Microbiol 42:45-51. 2004
  3. doi Neurovirulence and latency of drug-resistant clinical herpes simplex viruses in animal models
    Sarah Dambrosi
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Universite Laval, Quebec, Canada
    J Med Virol 82:1000-6. 2010
  4. doi Comparison of risk factors for human metapneumovirus and respiratory syncytial virus disease severity in young children
    Jesse Papenburg
    Centre de recherche en infectiologie de l Université Laval, Centre de recherche du CHUQ, Quebec, Canada
    J Infect Dis 206:178-89. 2012
  5. pmc Impact of mutations at residue I223 of the neuraminidase protein on the resistance profile, replication level, and virulence of the 2009 pandemic influenza virus
    Andrés Pizzorno
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 56:1208-14. 2012
  6. pmc Thymidine kinase mutations conferring acyclovir resistance in herpes simplex type 1 recombinant viruses
    Yan Sergerie
    CHUQ CHUL, Room RC 709, 2705, blvd Laurier, Sainte Foy, Quebec, Canada
    Antimicrob Agents Chemother 50:3889-92. 2006
  7. doi Incidence and characterization of cytomegalovirus resistance mutations among pediatric solid organ transplant patients who received valganciclovir prophylaxis
    Melanie Martin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, 2705 Blvd Laurier, Room RC 709, Quebec City, Quebec G1V 4G2, Canada
    J Clin Virol 47:321-4. 2010
  8. ncbi A reverse genetics study of resistance to neuraminidase inhibitors in an influenza A/H1N1 virus
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL, Quebec City, Canada
    Antivir Ther 9:577-81. 2004
  9. ncbi Infections by human coronavirus-NL in hospitalized children
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    Pediatr Infect Dis J 24:1045-8. 2005
  10. doi Prophylactic and therapeutic benefits of a monoclonal antibody against the fusion protein of human metapneumovirus in a mouse model
    Marie Eve Hamelin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, 2705 Laurier Blvd, Quebec City, QC, Canada G1V 4G2
    Antiviral Res 88:31-7. 2010

Collaborators

Detail Information

Publications115 found, 100 shown here

  1. ncbi Expression of the late cytomegalovirus (CMV) pp150 transcript in leukocytes of AIDS patients is associated with a high viral DNA load in leukocytes and presence of CMV DNA in plasma
    G Boivin
    Division of Microbiology and Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Sainte Foy, Quebec, Canada G1V 4G2
    J Infect Dis 179:1101-7. 1999
    ..Thus, active viral replication as determined by a high CMV DNA load in PBL is reflected by expression of the late CMV transcript in the same cells and by the presence of CMV DNA in plasma...
  2. pmc Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses
    Guy Boivin
    Research Center in Infectious Diseases of the CHUQ CHUL, Department of Pediatrics, Laval University, Quebec City, Canada
    J Clin Microbiol 42:45-51. 2004
    ..5 and 98.9% compared to 81.6 and 94.7% for the antigenic test. In conclusion, our multiplex real-time PCR assay combines both rapidity and sensitivity for detecting the most important respiratory viral pathogens in children...
  3. doi Neurovirulence and latency of drug-resistant clinical herpes simplex viruses in animal models
    Sarah Dambrosi
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Universite Laval, Quebec, Canada
    J Med Virol 82:1000-6. 2010
    ..A good correlation was generally found between replication kinetics of DNA pol mutants and their neurovirulence potential in mice whereas such correlation was not straightforward for TK mutants...
  4. doi Comparison of risk factors for human metapneumovirus and respiratory syncytial virus disease severity in young children
    Jesse Papenburg
    Centre de recherche en infectiologie de l Université Laval, Centre de recherche du CHUQ, Quebec, Canada
    J Infect Dis 206:178-89. 2012
    ..However, risk factors for severe hMPV disease remain unknown. We comparatively assessed environmental, host, and viral determinants for severe hMPV and RSV infections...
  5. pmc Impact of mutations at residue I223 of the neuraminidase protein on the resistance profile, replication level, and virulence of the 2009 pandemic influenza virus
    Andrés Pizzorno
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 56:1208-14. 2012
    ..In conclusion, amino acid changes at residue I223 may alter the NAI susceptibilities of pH1N1 variants without compromising fitness. Consequently, I223R and I223V mutations, alone or with H275Y, need to be thoroughly monitored...
  6. pmc Thymidine kinase mutations conferring acyclovir resistance in herpes simplex type 1 recombinant viruses
    Yan Sergerie
    CHUQ CHUL, Room RC 709, 2705, blvd Laurier, Sainte Foy, Quebec, Canada
    Antimicrob Agents Chemother 50:3889-92. 2006
    ..Alterations in both conserved and nonconserved regions of the TK gene were shown to confer high levels of resistance to ACV...
  7. doi Incidence and characterization of cytomegalovirus resistance mutations among pediatric solid organ transplant patients who received valganciclovir prophylaxis
    Melanie Martin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, 2705 Blvd Laurier, Room RC 709, Quebec City, Quebec G1V 4G2, Canada
    J Clin Virol 47:321-4. 2010
    ..Drug-resistant cytomegalovirus (CMV) infections can cause significant morbidity among high-risk transplant recipients...
  8. ncbi A reverse genetics study of resistance to neuraminidase inhibitors in an influenza A/H1N1 virus
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL, Quebec City, Canada
    Antivir Ther 9:577-81. 2004
    ..The replication capacity of both mutants, in particular that of the His274Tyr virus, was impaired when compared with the wild-type virus in vitro...
  9. ncbi Infections by human coronavirus-NL in hospitalized children
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    Pediatr Infect Dis J 24:1045-8. 2005
    ..Little information is available on the incidence, clinical manifestations and epidemiologic features of HCoV-NL infections...
  10. doi Prophylactic and therapeutic benefits of a monoclonal antibody against the fusion protein of human metapneumovirus in a mouse model
    Marie Eve Hamelin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, 2705 Laurier Blvd, Quebec City, QC, Canada G1V 4G2
    Antiviral Res 88:31-7. 2010
    ..Both prophylactic and, to a lesser extent, therapeutic administration of MAb 338 improved acute and late consequences of HMPV infection in a relevant mouse model...
  11. doi Effect of the neuraminidase mutation H274Y conferring resistance to oseltamivir on the replicative capacity and virulence of old and recent human influenza A(H1N1) viruses
    Mariana Baz
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 201:740-5. 2010
    ..Unexpectedly, an oseltamivir-resistant A(H1N1) variant containing the H274Y neuraminidase (NA) mutation recently disseminated worldwide, suggesting that the replication and virulence properties of this mutant virus were not compromised...
  12. doi Opposite effect of two cytomegalovirus DNA polymerase mutations on replicative capacity and polymerase activity
    Melanie Martin
    Centre Hospitalier Universitaire de Quebec, Quebec City, QC, Canada
    Antivir Ther 15:579-86. 2010
    ..The aim of this study was to characterize the role of two HCMV DNA polymerase mutations (K805Q and T821I) found in a ganciclovir- and foscarnet-resistant clinical isolate from an AIDS patient...
  13. doi Human respiratory syncytial virus and other viral infections in infants receiving palivizumab
    Guy Boivin
    Research Center in Infectious Diseases, CHUQ CHUL, Quebec City, QC, Canada
    J Clin Virol 42:52-7. 2008
    ..Palivizumab is a humanized monoclonal antibody that prevents severe human respiratory syncytial virus (HRSV) infections...
  14. ncbi Analysis of cytomegalovirus DNA polymerase (UL54) mutations in solid organ transplant patients receiving valganciclovir or ganciclovir prophylaxis
    Guy Boivin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    J Med Virol 77:425-9. 2005
    ..Thus, emergence of CMV UL54 mutations in the absence of UL97 mutations is a rare but possible event that is not necessarily associated with detrimental clinical outcome in solid organ transplant recipients...
  15. ncbi Characterization of drug-resistant recombinant influenza A/H1N1 viruses selected in vitro with peramivir and zanamivir
    Mariana Baz
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Que, Canada
    Antiviral Res 74:159-62. 2007
    ..The zanamivir-selected variant had a massive deletion in the region encoding the NA active center and an A200T hemagglutinin mutation. This variant exhibited reduced susceptibility to zanamivir with a drug-dependent phenotype...
  16. pmc Human bocavirus infections in hospitalized children and adults
    Jean Longtin
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 14:217-21. 2008
    ..Two clades of HBoV were identified. The pathogenic role of HBoV in RTIs is uncertain because it was frequently detected in symptomatic and asymptomatic children and was commonly found with other viruses in symptomatic children...
  17. pmc Evaluation of serological diagnostic methods for the 2009 pandemic influenza A (H1N1) virus
    Jesse Papenburg
    Infectious Disease Research Center of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Clin Vaccine Immunol 18:520-2. 2011
    ..Compared to hemagglutination inhibition, microneutralization demonstrated a higher level of concordance with RT-PCR (kappa = 0.69 versus kappa = 0.60) and greater sensitivity (83% versus 71%; P = 0.016)...
  18. pmc Effects of different adjuvants in the context of intramuscular and intranasal routes on humoral and cellular immune responses induced by detergent-split A/H3N2 influenza vaccines in mice
    Mariana Baz
    Research Center in Infectious Diseases of the CHUL, CHUQ and Department of Microbiology and Immunology of Laval University, Quebec, Canada
    Clin Vaccine Immunol 19:209-18. 2012
    ..Our data indicate that the adjuvanted vaccines tested in this study can elicit stronger, more persistent, and broader immune responses against A/H3N2 strains than nonadjuvanted inactivated influenza vaccines...
  19. pmc Recombinant phenotyping of cytomegalovirus UL54 mutations that emerged during cell passages in the presence of either ganciclovir or foscarnet
    Christian Gilbert
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 55:4019-27. 2011
    ....
  20. doi Reduced airborne transmission of oseltamivir-resistant pandemic A/H1N1 virus in ferrets
    Marie Eve Hamelin
    CHUQ CHUL Research Center in Infectious Diseases and Laval University, Quebec City, QC, Canada
    Antivir Ther 16:775-9. 2011
    ..We sought to evaluate transmission of this mutant virus through the direct contact and the airborne (aerosol and droplet) routes in the ferret model...
  21. pmc Oseltamivir-resistant pandemic A/H1N1 virus is as virulent as its wild-type counterpart in mice and ferrets
    Marie Eve Hamelin
    CHUQ CHUL Research Center in Infectious Diseases and Laval University, Quebec City, Quebec, Canada
    PLoS Pathog 6:e1001015. 2010
    ..Furthermore, the H274Y mutant strain was transmitted to ferrets. In conclusion, viral fitness of the H274Y pH1N1 isolate is not substantially altered and has the potential to induce severe disease and to disseminate...
  22. pmc The I222V neuraminidase mutation has a compensatory role in replication of an oseltamivir-resistant influenza virus A/H3N2 E119V mutant
    Philippe Simon
    Centre de Recherche en Infectiologie, Universite Laval, 2705 Boul Laurier, Quebec City, Quebec G1V 4G2, Canada
    J Clin Microbiol 49:715-7. 2011
    ..Based on plaque size, yield assays, and NA activity, the impaired viral fitness of the E119V mutant was partially restored by the I222V NA mutation...
  23. ncbi Distribution and clinical impact of human respiratory syncytial virus genotypes in hospitalized children over 2 winter seasons
    Rodica Gilca
    Laval University, Quebec, Canada
    J Infect Dis 193:54-8. 2006
    ..Group A and genotype GA2 strains were associated with greater severity of hRSV disease than were group B strains...
  24. ncbi Cytomegalovirus resistance in solid organ transplant recipients treated with intravenous ganciclovir or oral valganciclovir
    Guy Boivin
    Infectious Disease Research Center of the Centre hospitalier universitaire de Québec, Quebec City, QC, Canada
    Antivir Ther 14:697-704. 2009
    ..The rate of cytomegalovirus (CMV) mutations conferring ganciclovir resistance was assessed in a trial comparing intravenous ganciclovir and oral valganciclovir for treatment of CMV disease in solid organ transplant (SOT) recipients...
  25. pmc Identification and evaluation of a highly effective fusion inhibitor for human metapneumovirus
    Céline Deffrasnes
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 52:279-87. 2008
    ..Moreover, hMPV, compared to other paramyxoviruses and to the human immunodeficiency virus, seems to be more susceptible to HRA- than HRB-derived peptides...
  26. ncbi Hydroxyurea enhances the activity of acyclovir and cidofovir against herpes simplex virus type 1 resistant strains harboring mutations in the thymidine kinase and/or the DNA polymerase genes
    Yan Sergerie
    Research Center in Infectious Diseases of the CHUQ CHUL, Quebec City, QC, Canada
    Antiviral Res 77:77-80. 2008
    ..Hydroxyurea, a ribonucleotide reductase inhibitor, can increase the activity of nucleoside/nucleotide analogues against drug-resistant viruses...
  27. ncbi Discordant phenotypes and genotypes of cytomegalovirus (CMV) in patients with AIDS and relapsing CMV retinitis
    Christian Gilbert
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Laval, Laval University, 2705 boulevard Laurier Sainte Foy, Quebec City, Canada G1V 4G2
    AIDS 17:337-41. 2003
    ..To investigate the correlation between genotypic studies performed on blood leukocytes and phenotypic results obtained from the corresponding blood viral isolates in AIDS patients with relapsing cytomegalovirus (CMV) retinitis...
  28. ncbi Influenzavirus infection is a primary cause of febrile respiratory illness in HIV-infected adults, despite vaccination
    Marina B Klein
    Department of Medicine, Division of Infectious Diseases, Royal Victoria Hospital, McGill University Health Centre, Montreal, Quebec, Canada
    Clin Infect Dis 45:234-40. 2007
    ..We performed a surveillance study of outpatients who presented with fever and respiratory symptoms to examine the role of viral pathogens in these patients...
  29. pmc The H275Y neuraminidase mutation of the pandemic A/H1N1 influenza virus lengthens the eclipse phase and reduces viral output of infected cells, potentially compromising fitness in ferrets
    Lady Tatiana Pinilla
    Infectious Disease Research Centre, CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    J Virol 86:10651-60. 2012
    ..In ferrets, however, the mutant strain appears to suffer a delay in its infection of the respiratory tract that allows the susceptible strain to dominate mixed-strain infections...
  30. ncbi Characterization of human cytomegalovirus (HCMV) UL97 mutations found in a valganciclovir/oral ganciclovir prophylactic trial by use of a bacterial artificial chromosome containing the HCMV genome
    Melanie Martin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    J Infect Dis 194:579-83. 2006
    ..All recombinant viruses containing these unknown mutations were found to be susceptible to ganciclovir...
  31. doi Effect of pretreatment with toll-like receptor agonists in a mouse model of herpes simplex virus type 1 encephalitis
    Nicolas Boivin
    Research Center in Infectious Diseases, CHUQ CHUL, Quebec City, Canada
    J Infect Dis 198:664-72. 2008
    ..We evaluated the effect of pretreatment with Toll-like receptor (TLR) agonists in a mouse model of herpes simplex virus type 1 (HSV-1) encephalitis...
  32. doi Molecular and antigenic evolution of human influenza A/H3N2 viruses in Quebec, Canada, 2009-2011
    Julie Ann
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec, Quebec, Canada
    J Clin Virol 53:88-92. 2012
    ..A/H3N2 variability leads to poor vaccine effectiveness when the vaccine strain is not well matched to the circulating virus...
  33. pmc Contagious period for pandemic (H1N1) 2009
    Gaston De Serres
    Institut National de Sante Publique du Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 16:783-8. 2010
    ..Self-isolation only until fever abates appears insufficient to limit transmission. Self-isolation for a week may be more effective, although some case-patients still would shed infectious virus...
  34. pmc Activity of the oral neuraminidase inhibitor A-322278 against the oseltamivir-resistant H274Y (A/H1N1) influenza virus mutant in mice
    Mariana Baz
    Research Center in Infectious Diseases, CHUQ CHUL, Laval University, Quebec City, Canada
    Antimicrob Agents Chemother 53:791-3. 2009
    ..A-322278 decreased mortality rates and lung virus titers significantly more than oseltamivir in mice infected with the NA H274Y mutant when therapy was started 4 h before or even 48 h after infection...
  35. pmc Generation and characterization of recombinant pandemic influenza A(H1N1) viruses resistant to neuraminidase inhibitors
    Andrés Pizzorno
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 203:25-31. 2011
    ..Given the novel pandemic influenza A(H1N1) (pH1N1) virus and the restricted number of approved anti-influenza drugs, evaluation of potential drug-resistant variants is of high priority...
  36. pmc Prophylactic activity of intramuscular peramivir in mice infected with a recombinant influenza A/WSN/33 (H1N1) virus containing the H274Y neuraminidase mutation
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 54:2819-22. 2010
    ....
  37. pmc Human parechovirus types 1, 2 and 3 infections in Canada
    Yacine Abed
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 12:969-75. 2006
    ..Two infected patients (1 child with leukemia and a 78-year-old woman) died of septic shock and severe pneumonia, respectively...
  38. ncbi Longitudinal evaluation of herpes simplex virus DNA load during episodes of herpes labialis
    Guy Boivin
    Infectious Diseases Research Center, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    J Clin Virol 37:248-51. 2006
    ..Few studies have performed sequential evaluation of the herpes simplex virus (HSV) load using quantitative PCR during episodes of herpes labialis...
  39. ncbi Human metapneumovirus infection induces long-term pulmonary inflammation associated with airway obstruction and hyperresponsiveness in mice
    Marie Eve Hamelin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Laval University, Quebec City, Quebec, Canada
    J Infect Dis 193:1634-42. 2006
    ..The objective of the present work was to study the duration of pulmonary inflammation and the functional consequences of infection with hMPV by use of a BALB/c mouse model...
  40. pmc Generation and characterization of recombinant influenza A (H1N1) viruses harboring amantadine resistance mutations
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Canada
    Antimicrob Agents Chemother 49:556-9. 2005
    ..These findings could help explain the frequent emergence and transmission of amantadine-resistant influenza viruses during antiviral pressure in the clinical setting...
  41. pmc Evaluation of recombinant 2009 pandemic influenza A (H1N1) viruses harboring zanamivir resistance mutations in mice and ferrets
    Andrés Pizzorno
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 57:1784-9. 2013
    ..i. Our results demonstrate that zanamivir-resistant E119G and Q136K mutations compromise viral fitness and transmissibility in A(H1N1)pdm09 viruses...
  42. doi Incidence of cytomegalovirus UL97 and UL54 amino acid substitutions detected after 100 or 200 days of valganciclovir prophylaxis
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec, QC, Canada G1V 4G2
    J Clin Virol 53:208-13. 2012
    ..8% vs 200-day: 16.1%(1)), the consequence of extending the duration of prophylaxis on the development of viral resistance remains unknown...
  43. pmc Role of permissive neuraminidase mutations in influenza A/Brisbane/59/2007-like (H1N1) viruses
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    PLoS Pathog 7:e1002431. 2011
    ..In conclusion, the Q222R reversion mutation compromised Bris07-like H1N1 virus in vitro and in vivo. Thus, the R222Q NA mutation present in the WT virus may have facilitated the emergence of NAI-resistant Bris07 variants...
  44. pmc Molecular evolution of respiratory syncytial virus fusion gene, Canada, 2006-2010
    Jesse Papenburg
    McGill University Health Centre, Montreal, Quebec, Canada
    Emerg Infect Dis 18:120-4. 2012
    ..Resistance-conferring mutations within the palivizumab binding site occurred in 8.7% of palivizumab recipients and none of the nonrecipients...
  45. pmc Human metapneumovirus infections in hospitalized children
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec, Quebec City, Canada
    Emerg Infect Dis 9:634-40. 2003
    ..HMPV is an important cause of illness in young children with a similar, although less severe, clinical presentation to that of HRSV...
  46. pmc Development of a serological assay based on a synthetic peptide selected from the VP0 capsid protein for detection of human parechoviruses
    Yacine Abed
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    J Clin Microbiol 45:2037-9. 2007
    ..Seroprevalence for HPeVs was 70% in children of < or = 5 years of age and 95% in adults. For children from whom serial sera were sampled, seropositivity increased from 22% to 88% between 2 and 24 months of age...
  47. ncbi Inhibition of human metapneumovirus replication by small interfering RNA
    Céline Deffrasnes
    Infectious Disease Research Centre of the Centre Hospitalier Universitaire de Québec, Laval University, Quebec City, QC, Canada
    Antivir Ther 13:821-32. 2008
    ..Despite its major effects related to bronchiolitis, pneumonia and its potential role in recurrent wheezing episodes, there is still no commercial treatment or vaccine available against this paramyxovirus...
  48. doi The need for validation of statistical methods for estimating respiratory virus-attributable hospitalization
    Rodica Gilca
    Institut National de Sante Publique du Quebec, Quebec, PQ, Canada
    Am J Epidemiol 170:925-36. 2009
    ..Until these methods are validated, their methodological limitations should be made explicit and proxy estimates used cautiously in guiding public policy...
  49. doi Host and viral factors affecting clinical performance of a rapid diagnostic test for respiratory syncytial virus in hospitalized children
    Jesse Papenburg
    Department of Epidemiology, McGill University, Montreal, Quebec, Canada
    J Pediatr 163:911-3. 2013
    ..Older age, prolonged symptoms, and respiratory syncytial virus genotype-B infection were significantly associated with false-negative results of RADT. ..
  50. ncbi Pathogenicity and response to topical antiviral therapy in a murine model of acyclovir-sensitive and acyclovir-resistant herpes simplex viruses isolated from the same patient
    Annie Lebel
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, CHUQ CHUL, and Laval University, Quebec City, Que, Canada
    J Clin Virol 37:34-7. 2006
    ..Acyclovir-resistant herpes simplex viruses (HSV) are commonly recovered from immunocompromised individuals who exhibit chronic and/or disseminated herpetic lesions...
  51. ncbi Molecular characterization of a Canadian human parechovirus (HPeV)-3 isolate and its relationship to other HPeVs
    Yacine Abed
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    J Med Virol 77:566-70. 2005
    ..Phylogenetic analysis confirmed the relatedness between Can82853-01 and A308/99 and their divergence from other picornaviruses...
  52. doi Development of a universal influenza A vaccine based on the M2e peptide fused to the papaya mosaic virus (PapMV) vaccine platform
    Jérôme Denis
    Centre de Recherche en Infectiologie, Pavillon CHUL, Universite Laval, 2705 Boulevard Laurier, Quebec, PQ, G1V 4G2 Canada
    Vaccine 26:3395-403. 2008
    ..These results show, for the first time, the potential of a recombinant plant virus protein to serve as both peptide delivery system and adjuvant in the crucial field of influenza vaccine development...
  53. pmc Both TRIF and IPS-1 adaptor proteins contribute to the cerebral innate immune response against herpes simplex virus 1 infection
    Rafik Menasria
    Research Center in Infectious Diseases, CHUQ CHUL, and Department of Microbiology Infectiology and Immunology, Laval University, Quebec City, QC, Canada
    J Virol 87:7301-8. 2013
    ..These data suggest that both the TRIF and IPS-1 signaling pathways are important for the control of HSV replication in the brain and survival through IFN-β production...
  54. pmc Therapeutic activity of intramuscular peramivir in mice infected with a recombinant influenza A/WSN/33 (H1N1) virus containing the H275Y neuraminidase mutation
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 56:4375-80. 2012
    ..IM peramivir could constitute an alternative for treatment of oseltamivir-resistant A/H1N1 infections, although additional studies are warranted to support such a recommendation...
  55. pmc Comparative evaluation of real-time PCR assays for detection of the human metapneumovirus
    Stephanie Cote
    Infectious Diseases Research Center, Centre Hospitalier Universitaire de Québec CHUQ CHUL, Quebec City, Canada
    J Clin Microbiol 41:3631-5. 2003
    ..In conclusion, real-time PCR assays aimed at amplifying the N and L genes which are coding for two internal viral proteins appear particularly suitable for hMPV diagnostic...
  56. ncbi An outbreak of severe respiratory tract infection due to human metapneumovirus in a long-term care facility
    Guy Boivin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Clin Infect Dis 44:1152-8. 2007
    ..We sought to describe the epidemiological, virological, and histopathological findings associated with a large outbreak of hMPV infection in a long-term care facility...
  57. doi Antibody persistence and response to 2010-2011 trivalent influenza vaccine one year after a single dose of 2009 AS03-adjuvanted pandemic H1N1 vaccine in children
    Vladimir Gilca
    Quebec Public Health Institute, Canada
    Vaccine 30:35-41. 2011
    ..In 2009, several countries used the ASO3-adjuvanted pandemic A/H1N1 vaccine. We assessed the persistence of antibody and the priming induced by a single paediatric dose of this vaccine in children...
  58. ncbi Absence of cytomegalovirus-resistance mutations after valganciclovir prophylaxis, in a prospective multicenter study of solid-organ transplant recipients
    Guy Boivin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    J Infect Dis 189:1615-8. 2004
    ..Valganciclovir was associated with negligible risk of resistance and thus constitutes a useful alternative to ganciclovir prophylaxis for CMV in high-risk SOT recipients...
  59. doi The 2009 pandemic H1N1 D222G hemagglutinin mutation alters receptor specificity and increases virulence in mice but not in ferrets
    Yacine Abed
    Research Center in Infectious Diseases, CHUQ CHUL and Laval University, Quebec City, Canada
    J Infect Dis 204:1008-16. 2011
    ..We investigated the impact of this mutation in vitro and in animal models using recombinant pH1N1 viruses...
  60. doi Contrasting effects on ganciclovir susceptibility and replicative capacity of two mutations at codon 466 of the human cytomegalovirus UL97 gene
    Melanie Martin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    J Clin Virol 49:296-8. 2010
    ..Resistance to ganciclovir is predominantly associated with alterations in the HCMV UL97 kinase and, more occasionally, with mutations in the HCMV DNA polymerase gene...
  61. ncbi Impact of neuraminidase mutations conferring influenza resistance to neuraminidase inhibitors in the N1 and N2 genetic backgrounds
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antivir Ther 11:971-6. 2006
    ..It suggests that certain drug-resistant NA mutants may still be virulent although additional studies using clinical isolates are needed to confirm our results...
  62. ncbi Delayed but not early glucocorticoid treatment protects the host during experimental herpes simplex virus encephalitis in mice
    Yan Sergerie
    Centre Hospitalier de l Universite Laval Research Center and Laval University, Quebec, G1V 4G2, Canada
    J Infect Dis 195:817-25. 2007
    ..Glucocorticoids (GCs) have not been established as an evidence-based therapy for herpes simplex virus (HSV) encephalitis. We sought to evaluate the effects of early versus delayed GC administration in a model of HSV-1 encephalitis...
  63. pmc Seroconversion to seasonal influenza viruses after A(H1N1)pdm09 virus infection, Quebec, Canada
    Mariana Baz
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 18:1132-4. 2012
    ..05)...
  64. ncbi Enhanced lung disease and Th2 response following human metapneumovirus infection in mice immunized with the inactivated virus
    Marie Eve Hamelin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, 2705 Blvd Laurier, Quebec City, QC G1V 4G2, Canada
    J Gen Virol 88:3391-400. 2007
    ..Our data demonstrate the risks associated with immunization using inactivated hMPV in this animal model and that this aberrant response should be considered in the development of hMPV vaccines...
  65. pmc Infection with human metapneumovirus predisposes mice to severe pneumococcal pneumonia
    Irena Kukavica-Ibrulj
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    J Virol 83:1341-9. 2009
    ..Prior infection with either hMPV or influenza A virus predisposes mice to severe pneumococcus infection...
  66. ncbi Human metapneumovirus
    Céline Deffrasnes
    Department of Medical Biology, Faculty of Medicine, Laval University, Quebec, Canada
    Semin Respir Crit Care Med 28:213-21. 2007
    ..No commercial treatments are yet available for hMPV, although ribavirin has shown activity both in vitro and in animal models. Live attenuated vaccines produced by reverse genetics have also shown good efficacy in animals...
  67. ncbi Characterization of multidrug-resistant influenza A/H3N2 viruses shed during 1 year by an immunocompromised child
    Mariana Baz
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, G1V 4G2, Canada
    Clin Infect Dis 43:1555-61. 2006
    ..Development of influenza drug resistance is an important problem in immunocompromised children that could result in treatment failure and viral transmission to others...
  68. doi Modulation of TLR9 response in a mouse model of herpes simplex virus encephalitis
    Nicolas Boivin
    Research Center in Infectious Diseases, CHUQ CHUL and Laval University, Quebec City, QC, Canada
    Antiviral Res 96:414-21. 2012
    ..On the other hand, TLR9 antagonists can be helpful to control the inflammatory response that could be detrimental after infection...
  69. pmc Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children
    Frederic Raymond
    Infectious Disease Research Center of the CHUQ CHUL and Laval University, Sainte Foy, Quebec, Canada
    J Clin Microbiol 47:743-50. 2009
    ..The two methods yielded concordant results for 94.1% of specimens. These tests allowed a thorough etiological assessment of respiratory viruses infecting children in hospital settings and would assist public health interventions...
  70. doi Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
    Irena Kukavica-Ibrulj
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    J Clin Virol 44:88-90. 2009
    ..Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups...
  71. ncbi The prophylactic administration of a monoclonal antibody against human metapneumovirus attenuates viral disease and airways hyperresponsiveness in mice
    Marie Eve Hamelin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Laval University, Quebec City, Quebec, Canada
    Antivir Ther 13:39-46. 2008
    ..Our objective was to assess the prophylactic benefit of a monoclonal antibody (mAb) against the hMPV fusion protein in a murine model...
  72. ncbi Activity of the neuraminidase inhibitor A-315675 against oseltamivir-resistant influenza neuraminidases of N1 and N2 subtypes
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antiviral Res 77:163-6. 2008
    ..These results confirm the potent inhibitory effect of A-315675 against oseltamivir-resistant influenza viruses of the N1 and N2 subtypes and support the clinical development of its bioavailable prodrug A-322278...
  73. pmc New Saffold cardioviruses in 3 children, Canada
    Yacine Abed
    Centre Hospitalier Universitaire de Quebec, Quebec City, Canada
    Emerg Infect Dis 14:834-6. 2008
    ..Polyprotein sequence of the Can112051-06 isolate had 91.2% aa identity with Saffold virus; however, EF and CD loops of the viral surface varied substantially...
  74. ncbi Quantification of the influenza virus load by real-time polymerase chain reaction in nasopharyngeal swabs of patients treated with oseltamivir
    Guy Boivin
    Research Center in Infectious Diseases of CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 188:578-80. 2003
    ..3% vs. 20.7%). These virological results support the clinical benefit provided by early therapeutic intervention of influenza illness...
  75. pmc Resistance of herpes simplex viruses to nucleoside analogues: mechanisms, prevalence, and management
    Jocelyne Piret
    Centre de Recherche en Infectiologie, Universite Laval, Quebec, Quebec, Canada
    Antimicrob Agents Chemother 55:459-72. 2011
    ..There is a need to develop new antiherpetic compounds with different mechanisms of action...
  76. doi Long-term impairment of Streptococcus pneumoniae lung clearance is observed after initial infection with influenza A virus but not human metapneumovirus in mice
    Herbert P Ludewick
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, QC, G1V 4G2, Canada
    J Gen Virol 92:1662-5. 2011
    ..In conclusion, hMPV does not cause long-term impairment of pneumococcus lung clearance, in contrast to influenza A virus...
  77. ncbi Characterization of 2 influenza A(H3N2) clinical isolates with reduced susceptibility to neuraminidase inhibitors due to mutations in the hemagglutinin gene
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 186:1074-80. 2002
    ..Our study shows the importance of the HA1 229 residue in virus binding to MDCK cells and confirms the unreliability of cell-based assays in predicting the in vivo susceptibility of HA variants to NAIs...
  78. pmc WU polyomavirus in children, Canada
    Yacine Abed
    Centre Hospitalier Universitaire de Québec and Research Center in Infectious Diseases, Quebec City, Quebec, Canada
    Emerg Infect Dis 13:1939-41. 2007
    ..The strains were closely related to Australian and American viruses based on analysis of large T antigen (TAg) and VP2 genes. The pathogenic role of WU virus is still uncertain...
  79. doi Influenza drug resistance
    Andrés Pizzorno
    Research Center in Infectious Diseases, Centre hospitalier Univeritaire de Quebec and Laval University, Quebec City, Quebec, Canada
    Semin Respir Crit Care Med 32:409-22. 2011
    ..This highlights the need for continuous monitoring of resistance markers, as well as the development of new anti-influenza drugs and combination therapies...
  80. pmc Human parainfluenza type 4 infections, Canada
    Marie Louise Vachon
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 12:1755-8. 2006
  81. doi The distinguishing features of human metapneumovirus and respiratory syncytial virus
    Jesse Papenburg
    CHUQ CHUL and Université Laval, Quebec City, QC, Canada
    Rev Med Virol 20:245-60. 2010
    ....
  82. pmc Role of picornaviruses in flu-like illnesses of adults enrolled in an oseltamivir treatment study who had no evidence of influenza virus infection
    Guy Boivin
    Infectious Disease Research Center of the Centre hospitalier universitaire de Québec, Universite Laval, Quebec, Canada
    J Clin Microbiol 40:330-4. 2002
    ..We conclude that, among the influenza virus-negative preselected cases of this study, rhinoviruses were relatively frequent pathogens associated with important respiratory and systemic symptoms...
  83. ncbi Quantification of human herpesvirus 8 by real-time PCR in blood fractions of AIDS patients with Kaposi's sarcoma and multicentric Castleman's disease
    Guy Boivin
    Centre Hospitalier Universitaire de Québec CHUQ CHUL and Laval University, Quebec City, Canada
    J Med Virol 68:399-403. 2002
    ..These results suggest that the pathogenesis of the two HHV8-associated diseases (i.e., KS and MCD) might be different, as only the latter was associated with important viremia in our patients...
  84. ncbi Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups
    Guy Boivin
    Research Center in Infectious Diseases, Regional Virology Laboratory, Laval University, Quebec City, Canada
    J Infect Dis 186:1330-4. 2002
    ..These findings suggest that HMPV can be associated with severe lower-respiratory-tract infections in very young children, the elderly, and immunocompromised patients...
  85. ncbi Susceptibility of recent Canadian influenza A and B virus isolates to different neuraminidase inhibitors
    Guy Boivin
    Research Center in Infectious Diseases, CHUQ CHUL, Room RC 709, 2705 Blvd Laurier, Sainte Foy, Quebec, Canada G1V4G2
    Antiviral Res 54:143-7. 2002
    ..87 nM) viruses. Oseltamivir carboxylate was more active than zanamivir for influenza A(H3N2) isolates (mean IC(50): 0.73 vs. 2.09 nM) whereas it was less potent against B viruses (mean IC(50): 11.53 vs. 4.15 nM)...
  86. ncbi Prolonged excretion of amantadine-resistant influenza a virus quasi species after cessation of antiviral therapy in an immunocompromised patient
    Guy Boivin
    Research Center in Infectious Diseases, Centre hospitalier de l Universite Laval, Sainte Foy, Quebec, Canada
    Clin Infect Dis 34:E23-5. 2002
    ..These results confirm animal studies reported elsewhere regarding the genetic stability of influenza M2 mutants and their potential for transmission in humans...
  87. pmc Human parechovirus 3 and neonatal infections
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    Emerg Infect Dis 11:103-5. 2005
    ..6%-74.8%, 73.4%-73.6%, and 97.0%-97.1% when compared to HPeV-1, -2, and -3 prototype strains, respectively. Although different, our isolates were closely related; amino acid identity was 99.6%-100% for the last 3 proteins...
  88. ncbi Diagnosis of herpesvirus infections of the central nervous system
    Guy Boivin
    Laboratoire d infectiologie, Pavillon CHUL, Universite Laval, Canada
    Herpes 11:48A-56A. 2004
    ..This article presents the current evidence for these and other guidelines for the diagnosis of herpesvirus infections of the CNS...
  89. doi A novel neuraminidase deletion mutation conferring resistance to oseltamivir in clinical influenza A/H3N2 virus
    Yacine Abed
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Québec Centre Hospitalier de l Université Laval and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 199:180-3. 2009
    ..This study highlights the diversity of mechanisms of resistance to oseltamivir in clinical settings, reinforcing the need for novel anti-influenza strategies...
  90. ncbi Functional ecology of immature parasitoids
    Jacques Brodeur
    Departement de Phytologie, Universite Laval, Quebec, Canada G1K 7P4
    Annu Rev Entomol 49:27-49. 2004
    ....
  91. pmc Global genetic diversity of human metapneumovirus fusion gene
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Sainte Foy, Quebec City, Canada
    Emerg Infect Dis 10:1154-7. 2004
    ..Although group A strains predominated, accounting for 69% of all strains, as many B as A strains were found in persons >3 years of age...
  92. ncbi Estradiol is required for a proper immune response to bacterial and viral pathogens in the female brain
    Genevieve Soucy
    Laboratory of Molecular Endocrinology, Centre Hospitalier de l Universite Laval Research Center, Quebec City, Quebec, Canada
    J Immunol 174:6391-8. 2005
    ..This transfer is deviant in mice lacking E(2), which allows pathogens to hide from immune surveillance and exacerbates neuronal damages during viral encephalitis...
  93. ncbi Human metapneumovirus: a new player among respiratory viruses
    Marie Eve Hamelin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, and Laval University, Quebec City, Canada
    Clin Infect Dis 38:983-90. 2004
    ..Additional research is needed to define the pathogenesis of this viral infection and the host's specific immune response...
  94. pmc Role of helix P of the human cytomegalovirus DNA polymerase in resistance and hypersusceptibility to the antiviral drug foscarnet
    Egor P Tchesnokov
    McGill University, Department of Microbiology and Immunology, Room D 6, Duff Medical Building, 3775 University Street, Montreal, Quebec, Canada H3A 2B4
    J Virol 80:1440-50. 2006
    ..In contrast, the removal of a positive charge associated with the K805Q change may facilitate access or increase affinity to the adjacent Q807...
  95. pmc Multi-year study of human metapneumovirus infection at a large US Midwestern Medical Referral Center
    Gregory C Gray
    Center for Emerging Infectious Diseases, Department of Epidemiology, University of Iowa College of Public Health, Iowa City, 200 Hawkins Dr, C21 K GH, Iowa City, IA 52242, USA
    J Clin Virol 37:269-76. 2006
    ..Because of its recent identification, few multi-year epidemiologic studies of hMPV infection have been reported...
  96. ncbi Characterization of human metapneumoviruses isolated from patients in North America
    Teresa C T Peret
    Respiratory Virus Section, Respiratory and Enteric Viruses Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA
    J Infect Dis 185:1660-3. 2002
    ..These findings support a previous finding that HMPV is a human respiratory pathogen that merits further study...
  97. pmc Analysis of replication kinetics of the human metapneumovirus in different cell lines by real-time PCR
    Céline Deffrasnes
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, and Laval University, Canada
    J Clin Microbiol 43:488-90. 2005
    ..Using a quantitative real-time TaqMan PCR, we analyzed the replication kinetics of hMPV in different cell lines. Our results indicate that hMPV replicates slightly more efficiently in LLC-MK2 than in Vero cells and poorly in HEp-2 cells...
  98. ncbi Syncytium formation and HIV-1 replication are both accentuated by purified influenza and virus-associated neuraminidase
    Jiangfeng Sun
    Centre de Recherche en Infectiologie, Hôpital du Centre Hospitalier Universitaire de L Université Laval, Quebec G1V 4G2, Canada
    J Biol Chem 277:9825-33. 2002
    ..Our results provide a new model allowing the investigation of the potential benefit of using NA inhibitors in the treatment of HIV-1-infected patients suffering from coinfection with NA-bearing pathogens...
  99. ncbi Influenza virus activates human immunodeficiency virus type-1 gene expression in human CD4-expressing T cells through an NF-kappaB-dependent pathway
    Jiangfeng Sun
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
    Clin Immunol 114:190-8. 2005
    ..Our data show that fully competent Flu can lead to NF-kappaB-dependent activation of HIV-1 transcription in CD4+ T cells...
  100. pmc Human metapneumovirus, Peru
    Gregory C Gray
    University of Iowa College of Public Health, Iowa City, Iowa 52242, USA
    Emerg Infect Dis 12:347-50. 2006
    ..Twelve specimens (2.3%) were positive by multiple assays. Six specimens yielded HMPV isolates. Four of the 6 isolates were of the uncommon B1 genotype...
  101. pmc Effect of ribavirin and glucocorticoid treatment in a mouse model of human metapneumovirus infection
    Marie Eve Hamelin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Sainte Foy, Quebec, Canada
    Antimicrob Agents Chemother 50:774-7. 2006
    ..Ribavirin significantly decreased both hMPV replication in lungs (by 5 log10) and global pulmonary inflammation on day 5 postinfection, whereas glucocorticoids reduced only alveolar and interstitial inflammation, compared to controls...