G Boivin

Summary

Country: Canada

Publications

  1. doi request reprint Neurovirulence and latency of drug-resistant clinical herpes simplex viruses in animal models
    Sarah Dambrosi
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Universite Laval, Quebec, Canada
    J Med Virol 82:1000-6. 2010
  2. ncbi request reprint Diagnosis of herpesvirus infections of the central nervous system
    Guy Boivin
    Laboratoire d infectiologie, Pavillon CHUL, Universite Laval, Canada
    Herpes 11:48A-56A. 2004
  3. ncbi request reprint Expression of the late cytomegalovirus (CMV) pp150 transcript in leukocytes of AIDS patients is associated with a high viral DNA load in leukocytes and presence of CMV DNA in plasma
    G Boivin
    Division of Microbiology and Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Sainte Foy, Quebec, Canada G1V 4G2
    J Infect Dis 179:1101-7. 1999
  4. ncbi request reprint Inhibition of human metapneumovirus replication by small interfering RNA
    Céline Deffrasnes
    Infectious Disease Research Centre of the Centre Hospitalier Universitaire de Québec, Laval University, Quebec City, QC, Canada
    Antivir Ther 13:821-32. 2008
  5. pmc Identification and evaluation of a highly effective fusion inhibitor for human metapneumovirus
    Céline Deffrasnes
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 52:279-87. 2008
  6. pmc Human bocavirus infections in hospitalized children and adults
    Jean Longtin
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 14:217-21. 2008
  7. pmc Respiratory tract reinfections by the new human Metapneumovirus in an immunocompromised child
    Gilles Pelletier
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec, Canada
    Emerg Infect Dis 8:976-8. 2002
  8. pmc Human parechovirus types 1, 2 and 3 infections in Canada
    Yacine Abed
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 12:969-75. 2006
  9. pmc Seroconversion to seasonal influenza viruses after A(H1N1)pdm09 virus infection, Quebec, Canada
    Mariana Baz
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 18:1132-4. 2012
  10. pmc Role of permissive neuraminidase mutations in influenza A/Brisbane/59/2007-like (H1N1) viruses
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    PLoS Pathog 7:e1002431. 2011

Collaborators

Detail Information

Publications97

  1. doi request reprint Neurovirulence and latency of drug-resistant clinical herpes simplex viruses in animal models
    Sarah Dambrosi
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Universite Laval, Quebec, Canada
    J Med Virol 82:1000-6. 2010
    ..A good correlation was generally found between replication kinetics of DNA pol mutants and their neurovirulence potential in mice whereas such correlation was not straightforward for TK mutants...
  2. ncbi request reprint Diagnosis of herpesvirus infections of the central nervous system
    Guy Boivin
    Laboratoire d infectiologie, Pavillon CHUL, Universite Laval, Canada
    Herpes 11:48A-56A. 2004
    ..This article presents the current evidence for these and other guidelines for the diagnosis of herpesvirus infections of the CNS...
  3. ncbi request reprint Expression of the late cytomegalovirus (CMV) pp150 transcript in leukocytes of AIDS patients is associated with a high viral DNA load in leukocytes and presence of CMV DNA in plasma
    G Boivin
    Division of Microbiology and Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Sainte Foy, Quebec, Canada G1V 4G2
    J Infect Dis 179:1101-7. 1999
    ..Thus, active viral replication as determined by a high CMV DNA load in PBL is reflected by expression of the late CMV transcript in the same cells and by the presence of CMV DNA in plasma...
  4. ncbi request reprint Inhibition of human metapneumovirus replication by small interfering RNA
    Céline Deffrasnes
    Infectious Disease Research Centre of the Centre Hospitalier Universitaire de Québec, Laval University, Quebec City, QC, Canada
    Antivir Ther 13:821-32. 2008
    ..Despite its major effects related to bronchiolitis, pneumonia and its potential role in recurrent wheezing episodes, there is still no commercial treatment or vaccine available against this paramyxovirus...
  5. pmc Identification and evaluation of a highly effective fusion inhibitor for human metapneumovirus
    Céline Deffrasnes
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 52:279-87. 2008
    ..Moreover, hMPV, compared to other paramyxoviruses and to the human immunodeficiency virus, seems to be more susceptible to HRA- than HRB-derived peptides...
  6. pmc Human bocavirus infections in hospitalized children and adults
    Jean Longtin
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 14:217-21. 2008
    ..Two clades of HBoV were identified. The pathogenic role of HBoV in RTIs is uncertain because it was frequently detected in symptomatic and asymptomatic children and was commonly found with other viruses in symptomatic children...
  7. pmc Respiratory tract reinfections by the new human Metapneumovirus in an immunocompromised child
    Gilles Pelletier
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec, Canada
    Emerg Infect Dis 8:976-8. 2002
    ..We report the case of a young, immunocompromised child who had severe lower respiratory tract infections during two consecutive winter seasons caused by genetically distinct HMPV strains...
  8. pmc Human parechovirus types 1, 2 and 3 infections in Canada
    Yacine Abed
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 12:969-75. 2006
    ..Two infected patients (1 child with leukemia and a 78-year-old woman) died of septic shock and severe pneumonia, respectively...
  9. pmc Seroconversion to seasonal influenza viruses after A(H1N1)pdm09 virus infection, Quebec, Canada
    Mariana Baz
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 18:1132-4. 2012
    ..05)...
  10. pmc Role of permissive neuraminidase mutations in influenza A/Brisbane/59/2007-like (H1N1) viruses
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    PLoS Pathog 7:e1002431. 2011
    ..In conclusion, the Q222R reversion mutation compromised Bris07-like H1N1 virus in vitro and in vivo. Thus, the R222Q NA mutation present in the WT virus may have facilitated the emergence of NAI-resistant Bris07 variants...
  11. pmc Modulation of protease activated receptor 1 influences human metapneumovirus disease severity in a mouse model
    Laetitia Aerts
    Centre de Recherche en Infectiologie du Centre Hospitalier Universitaire de Québec and Université Laval, Quebec, Canada
    PLoS ONE 8:e72529. 2013
    ..Thus, inhibition of PAR1 by selected antagonists could represent an interesting strategy for decreasing the severity of paramyxovirus infections. ..
  12. pmc Human parainfluenza type 4 infections, Canada
    Marie Louise Vachon
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 12:1755-8. 2006
  13. pmc Molecular evolution of respiratory syncytial virus fusion gene, Canada, 2006-2010
    Jesse Papenburg
    McGill University Health Centre, Montreal, Quebec, Canada
    Emerg Infect Dis 18:120-4. 2012
    ..Resistance-conferring mutations within the palivizumab binding site occurred in 8.7% of palivizumab recipients and none of the nonrecipients...
  14. pmc Human metapneumovirus infection in immunocompromised child
    Yacine Abed
    Emerg Infect Dis 14:854-6. 2008
  15. pmc New Saffold cardioviruses in 3 children, Canada
    Yacine Abed
    Centre Hospitalier Universitaire de Quebec, Quebec City, Canada
    Emerg Infect Dis 14:834-6. 2008
    ..Polyprotein sequence of the Can112051-06 isolate had 91.2% aa identity with Saffold virus; however, EF and CD loops of the viral surface varied substantially...
  16. pmc WU polyomavirus in children, Canada
    Yacine Abed
    Centre Hospitalier Universitaire de Québec and Research Center in Infectious Diseases, Quebec City, Quebec, Canada
    Emerg Infect Dis 13:1939-41. 2007
    ..The strains were closely related to Australian and American viruses based on analysis of large T antigen (TAg) and VP2 genes. The pathogenic role of WU virus is still uncertain...
  17. doi request reprint Modulation of TLR9 response in a mouse model of herpes simplex virus encephalitis
    Nicolas Boivin
    Research Center in Infectious Diseases, CHUQ CHUL and Laval University, Quebec City, QC, Canada
    Antiviral Res 96:414-21. 2012
    ..On the other hand, TLR9 antagonists can be helpful to control the inflammatory response that could be detrimental after infection...
  18. pmc Impact of mutations at residue I223 of the neuraminidase protein on the resistance profile, replication level, and virulence of the 2009 pandemic influenza virus
    Andrés Pizzorno
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 56:1208-14. 2012
    ..In conclusion, amino acid changes at residue I223 may alter the NAI susceptibilities of pH1N1 variants without compromising fitness. Consequently, I223R and I223V mutations, alone or with H275Y, need to be thoroughly monitored...
  19. pmc Effects of different adjuvants in the context of intramuscular and intranasal routes on humoral and cellular immune responses induced by detergent-split A/H3N2 influenza vaccines in mice
    Mariana Baz
    Research Center in Infectious Diseases of the CHUL, CHUQ and Department of Microbiology and Immunology of Laval University, Quebec, Canada
    Clin Vaccine Immunol 19:209-18. 2012
    ..Our data indicate that the adjuvanted vaccines tested in this study can elicit stronger, more persistent, and broader immune responses against A/H3N2 strains than nonadjuvanted inactivated influenza vaccines...
  20. pmc Systematic review of influenza resistance to the neuraminidase inhibitors
    Kristian Thorlund
    Department of Clinical Epidemiology and Biostatistics, Faculty of Health Sciences, McMaster University, Hamilton, L8N 3Z5 Ontario, Canada
    BMC Infect Dis 11:134. 2011
    ....
  21. pmc Human parechovirus 3 and neonatal infections
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    Emerg Infect Dis 11:103-5. 2005
    ..6%-74.8%, 73.4%-73.6%, and 97.0%-97.1% when compared to HPeV-1, -2, and -3 prototype strains, respectively. Although different, our isolates were closely related; amino acid identity was 99.6%-100% for the last 3 proteins...
  22. doi request reprint Effect of pretreatment with toll-like receptor agonists in a mouse model of herpes simplex virus type 1 encephalitis
    Nicolas Boivin
    Research Center in Infectious Diseases, CHUQ CHUL, Quebec City, Canada
    J Infect Dis 198:664-72. 2008
    ..We evaluated the effect of pretreatment with Toll-like receptor (TLR) agonists in a mouse model of herpes simplex virus type 1 (HSV-1) encephalitis...
  23. ncbi request reprint Longitudinal evaluation of herpes simplex virus DNA load during episodes of herpes labialis
    Guy Boivin
    Infectious Diseases Research Center, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    J Clin Virol 37:248-51. 2006
    ..Few studies have performed sequential evaluation of the herpes simplex virus (HSV) load using quantitative PCR during episodes of herpes labialis...
  24. pmc Global genetic diversity of human metapneumovirus fusion gene
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Sainte Foy, Quebec City, Canada
    Emerg Infect Dis 10:1154-7. 2004
    ..Although group A strains predominated, accounting for 69% of all strains, as many B as A strains were found in persons >3 years of age...
  25. ncbi request reprint Analysis of cytomegalovirus DNA polymerase (UL54) mutations in solid organ transplant patients receiving valganciclovir or ganciclovir prophylaxis
    Guy Boivin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    J Med Virol 77:425-9. 2005
    ..Thus, emergence of CMV UL54 mutations in the absence of UL97 mutations is a rare but possible event that is not necessarily associated with detrimental clinical outcome in solid organ transplant recipients...
  26. ncbi request reprint Infections by human coronavirus-NL in hospitalized children
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    Pediatr Infect Dis J 24:1045-8. 2005
    ..Little information is available on the incidence, clinical manifestations and epidemiologic features of HCoV-NL infections...
  27. ncbi request reprint Cytomegalovirus resistance in solid organ transplant recipients treated with intravenous ganciclovir or oral valganciclovir
    Guy Boivin
    Infectious Disease Research Center of the Centre hospitalier universitaire de Québec, Quebec City, QC, Canada
    Antivir Ther 14:697-704. 2009
    ..The rate of cytomegalovirus (CMV) mutations conferring ganciclovir resistance was assessed in a trial comparing intravenous ganciclovir and oral valganciclovir for treatment of CMV disease in solid organ transplant (SOT) recipients...
  28. doi request reprint Human respiratory syncytial virus and other viral infections in infants receiving palivizumab
    Guy Boivin
    Research Center in Infectious Diseases, CHUQ CHUL, Quebec City, QC, Canada
    J Clin Virol 42:52-7. 2008
    ..Palivizumab is a humanized monoclonal antibody that prevents severe human respiratory syncytial virus (HRSV) infections...
  29. ncbi request reprint Clinical impact of ganciclovir-resistant cytomegalovirus infections in solid organ transplant patients
    G Boivin
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Transpl Infect Dis 7:166-70. 2005
    ..Additionally, no CMV resistance mutations were observed in non-lung transplant patients receiving valganciclovir...
  30. pmc Seasonal cholera from multiple small outbreaks, rural Bangladesh
    O Colin Stine
    University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Emerg Infect Dis 14:831-3. 2008
    ..The observed minimal overlap in VNTR patterns between the 2 communities was consistent with sequential, small outbreaks from local sources...
  31. ncbi request reprint An outbreak of severe respiratory tract infection due to human metapneumovirus in a long-term care facility
    Guy Boivin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Clin Infect Dis 44:1152-8. 2007
    ..We sought to describe the epidemiological, virological, and histopathological findings associated with a large outbreak of hMPV infection in a long-term care facility...
  32. ncbi request reprint Characterization of multidrug-resistant influenza A/H3N2 viruses shed during 1 year by an immunocompromised child
    Mariana Baz
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, G1V 4G2, Canada
    Clin Infect Dis 43:1555-61. 2006
    ..Development of influenza drug resistance is an important problem in immunocompromised children that could result in treatment failure and viral transmission to others...
  33. ncbi request reprint Absence of cytomegalovirus-resistance mutations after valganciclovir prophylaxis, in a prospective multicenter study of solid-organ transplant recipients
    Guy Boivin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    J Infect Dis 189:1615-8. 2004
    ..Valganciclovir was associated with negligible risk of resistance and thus constitutes a useful alternative to ganciclovir prophylaxis for CMV in high-risk SOT recipients...
  34. pmc Human herpesvirus 8 DNA load in leukocytes of human immunodeficiency virus-infected subjects: correlation with the presence of Kaposi's sarcoma and response to anticytomegalovirus therapy
    G Boivin
    Infectious Disease Research Center of the Centre Hospitalier de l Université Laval, Quebec City, Canada
    Antimicrob Agents Chemother 43:377-80. 1999
    ..The use of intravenous ganciclovir or foscarnet therapy to treat cytomegalovirus disease did not affect the HHV-8 DNA load in seven patients for whom serial samples were analyzed...
  35. pmc Role of picornaviruses in flu-like illnesses of adults enrolled in an oseltamivir treatment study who had no evidence of influenza virus infection
    Guy Boivin
    Infectious Disease Research Center of the Centre hospitalier universitaire de Québec, Universite Laval, Quebec, Canada
    J Clin Microbiol 40:330-4. 2002
    ..We conclude that, among the influenza virus-negative preselected cases of this study, rhinoviruses were relatively frequent pathogens associated with important respiratory and systemic symptoms...
  36. ncbi request reprint Rate of emergence of cytomegalovirus (CMV) mutations in leukocytes of patients with acquired immunodeficiency syndrome who are receiving valganciclovir as induction and maintenance therapy for CMV retinitis
    G Boivin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Sainte Foy, Quebec, Canada G1V 4G2
    J Infect Dis 184:1598-602. 2001
    ..The rate of emergence of ganciclovir-resistant viruses with use of oral valganciclovir is no greater than that reported with use of intravenous ganciclovir...
  37. ncbi request reprint Susceptibility of recent Canadian influenza A and B virus isolates to different neuraminidase inhibitors
    Guy Boivin
    Research Center in Infectious Diseases, CHUQ CHUL, Room RC 709, 2705 Blvd Laurier, Sainte Foy, Quebec, Canada G1V4G2
    Antiviral Res 54:143-7. 2002
    ..87 nM) viruses. Oseltamivir carboxylate was more active than zanamivir for influenza A(H3N2) isolates (mean IC(50): 0.73 vs. 2.09 nM) whereas it was less potent against B viruses (mean IC(50): 11.53 vs. 4.15 nM)...
  38. ncbi request reprint Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups
    Guy Boivin
    Research Center in Infectious Diseases, Regional Virology Laboratory, Laval University, Quebec City, Canada
    J Infect Dis 186:1330-4. 2002
    ..These findings suggest that HMPV can be associated with severe lower-respiratory-tract infections in very young children, the elderly, and immunocompromised patients...
  39. pmc Human metapneumovirus infections in hospitalized children
    Guy Boivin
    Centre Hospitalier Universitaire de Quebec, Quebec City, Canada
    Emerg Infect Dis 9:634-40. 2003
    ..HMPV is an important cause of illness in young children with a similar, although less severe, clinical presentation to that of HRSV...
  40. pmc Evaluation of the AMPLICOR cytomegalovirus test with specimens from human immunodeficiency virus-infected subjects
    G Boivin
    Research Center in Infections Diseases CHUL, Quebec City, Montreal, Canada
    J Clin Microbiol 36:2509-13. 1998
    ..A positive result by this test seems particularly important since this implies, in most instances, the presence or the imminence of CMV disease, although a negative test result does not rule out disease...
  41. ncbi request reprint Prolonged excretion of amantadine-resistant influenza a virus quasi species after cessation of antiviral therapy in an immunocompromised patient
    Guy Boivin
    Research Center in Infectious Diseases, Centre hospitalier de l Universite Laval, Sainte Foy, Quebec, Canada
    Clin Infect Dis 34:E23-5. 2002
    ..These results confirm animal studies reported elsewhere regarding the genetic stability of influenza M2 mutants and their potential for transmission in humans...
  42. pmc Evaluation of a rapid optical immunoassay for influenza viruses (FLU OIA test) in comparison with cell culture and reverse transcription-PCR
    G Boivin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    J Clin Microbiol 39:730-2. 2001
    ..The rate of positivity of the FLU OIA test (41.5%) was significantly lower than that of cell culture (55.2%) or reverse transcription-PCR (55.9%) during a season in which only influenza A virus was detected...
  43. ncbi request reprint Predicting influenza infections during epidemics with use of a clinical case definition
    G Boivin
    Research Center in Infectious Diseases and Centre Hospitalier Universitaire de Québec, Quebec City, Quebec, Canada
    Clin Infect Dis 31:1166-9. 2000
    ..8%, 39.3%, 77.6%, and 55.0%, respectively...
  44. ncbi request reprint Quantification of the influenza virus load by real-time polymerase chain reaction in nasopharyngeal swabs of patients treated with oseltamivir
    Guy Boivin
    Research Center in Infectious Diseases of CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 188:578-80. 2003
    ..3% vs. 20.7%). These virological results support the clinical benefit provided by early therapeutic intervention of influenza illness...
  45. ncbi request reprint Rapid antiviral effect of inhaled zanamivir in the treatment of naturally occurring influenza in otherwise healthy adults
    G Boivin
    Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Sainte Foy, Quebec, Canada
    J Infect Dis 181:1471-4. 2000
    ..03 after adjusting for the initial virus titer and the time between onset of symptoms and treatment). Resistance to zanamivir was not detected in virus isolates by either phenotypic or genotypic assays...
  46. ncbi request reprint A reverse genetics study of resistance to neuraminidase inhibitors in an influenza A/H1N1 virus
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL, Quebec City, Canada
    Antivir Ther 9:577-81. 2004
    ..The replication capacity of both mutants, in particular that of the His274Tyr virus, was impaired when compared with the wild-type virus in vitro...
  47. pmc Thymidine kinase mutations conferring acyclovir resistance in herpes simplex type 1 recombinant viruses
    Yan Sergerie
    CHUQ CHUL, Room RC 709, 2705, blvd Laurier, Sainte Foy, Quebec, Canada
    Antimicrob Agents Chemother 50:3889-92. 2006
    ..Alterations in both conserved and nonconserved regions of the TK gene were shown to confer high levels of resistance to ACV...
  48. ncbi request reprint Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997-2000 period
    I Hardy
    Infectious Disease Research Center, , Pavillon, CHUL RC-709, 2705 Blvd Laurier, , GIV 4G2, Sainte-Foy, Canada
    Virus Res 77:89-96. 2001
    ..Our studies suggest that molecular analysis of the HA1 gene should complement the HI assay for a more accurate analysis of influenza A virus drift...
  49. doi request reprint Incidence and characterization of cytomegalovirus resistance mutations among pediatric solid organ transplant patients who received valganciclovir prophylaxis
    Melanie Martin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, 2705 Blvd Laurier, Room RC 709, Quebec City, Quebec G1V 4G2, Canada
    J Clin Virol 47:321-4. 2010
    ..Drug-resistant cytomegalovirus (CMV) infections can cause significant morbidity among high-risk transplant recipients...
  50. ncbi request reprint Discordant phenotypes and genotypes of cytomegalovirus (CMV) in patients with AIDS and relapsing CMV retinitis
    Christian Gilbert
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Laval, Laval University, 2705 boulevard Laurier Sainte Foy, Quebec City, Canada G1V 4G2
    AIDS 17:337-41. 2003
    ..To investigate the correlation between genotypic studies performed on blood leukocytes and phenotypic results obtained from the corresponding blood viral isolates in AIDS patients with relapsing cytomegalovirus (CMV) retinitis...
  51. pmc New reporter cell line to evaluate the sequential emergence of multiple human cytomegalovirus mutations during in vitro drug exposure
    C Gilbert
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Canada
    Antimicrob Agents Chemother 49:4860-6. 2005
    ..e., ganciclovir, cidofovir, and foscarnet. Emergence of each viral mutation conferred stepwise increases in drug 50% inhibitory concentrations that could be objectively measured with the new reporter cell assay...
  52. ncbi request reprint Case study: rapid emergence of a cytomegalovirus UL97 mutant in a heart-transplant recipient on pre-emptive ganciclovir therapy
    C Gilbert
    Infectious Disease Research Center of the Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    Herpes 8:80-2. 2001
    ..Frequent monitoring of the CMV viral load and/or mutational studies are warranted in highly immunocompromised transplant recipients to prevent the development of CMV disease caused by ganciclovir-resistant strains...
  53. ncbi request reprint Characterization of the DNA polymerase and thymidine kinase genesof herpes simplex virus isolates from AIDS patients in whom acyclovirand foscarnet therapy sequentially failed
    I Schmit
    Infectious Disease Research Center, Centre hospitalier de l Universite Laval, Quebec, Canada, G1V 4G2
    J Infect Dis 180:487-90. 1999
    ..In this study of clinical HSV isolates, DNA pol mutations conferring foscarnet resistance were not associated with decreased acyclovir or cidofovir susceptibility...
  54. ncbi request reprint Pathogenicity and response to topical antiviral therapy in a murine model of acyclovir-sensitive and acyclovir-resistant herpes simplex viruses isolated from the same patient
    Annie Lebel
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, CHUQ CHUL, and Laval University, Quebec City, Que, Canada
    J Clin Virol 37:34-7. 2006
    ..Acyclovir-resistant herpes simplex viruses (HSV) are commonly recovered from immunocompromised individuals who exhibit chronic and/or disseminated herpetic lesions...
  55. ncbi request reprint Human metapneumovirus infection induces long-term pulmonary inflammation associated with airway obstruction and hyperresponsiveness in mice
    Marie Eve Hamelin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec, Laval University, Quebec City, Quebec, Canada
    J Infect Dis 193:1634-42. 2006
    ..The objective of the present work was to study the duration of pulmonary inflammation and the functional consequences of infection with hMPV by use of a BALB/c mouse model...
  56. pmc Drug resistance patterns of recombinant herpes simplex virus DNA polymerase mutants generated with a set of overlapping cosmids and plasmids
    Julie Bestman-Smith
    Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Québec Pavillon CHUL and Université Laval, 2705 Boulevard Laurier, Ste Foy, Quebec, Canada G1V 4G2
    J Virol 77:7820-9. 2003
    ..This new system allows a rapid and accurate evaluation of the functional role of various DNA Pol mutations, which should translate into improved management of drug-resistant HSV infections...
  57. pmc Prophylactic activity of intramuscular peramivir in mice infected with a recombinant influenza A/WSN/33 (H1N1) virus containing the H274Y neuraminidase mutation
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 54:2819-22. 2010
    ....
  58. pmc Evaluation of susceptibility of human herpesvirus 8 to antiviral drugs by quantitative real-time PCR
    Yan Sergerie
    Centre de Recherche en Infectiologie, Centre hospitalier universitaire de Québec Pavillon CHUL, and Université Laval, Quebec, Canada
    J Clin Microbiol 41:3897-900. 2003
    ..61 microM), adefovir (18.00 microM), acyclovir (31.00 microM), and foscarnet (34.15 microM). The potential therapeutic efficacy for HHV-8 (i.e., peak serum drug level/IC(50)) is highest for cidofovir (167) and foscarnet (22)...
  59. pmc Comparative evaluation of real-time PCR assays for detection of the human metapneumovirus
    Stephanie Cote
    Infectious Diseases Research Center, Centre Hospitalier Universitaire de Québec CHUQ CHUL, Quebec City, Canada
    J Clin Microbiol 41:3631-5. 2003
    ..In conclusion, real-time PCR assays aimed at amplifying the N and L genes which are coding for two internal viral proteins appear particularly suitable for hMPV diagnostic...
  60. ncbi request reprint Distribution and clinical impact of human respiratory syncytial virus genotypes in hospitalized children over 2 winter seasons
    Rodica Gilca
    Laval University, Quebec, Canada
    J Infect Dis 193:54-8. 2006
    ..Group A and genotype GA2 strains were associated with greater severity of hRSV disease than were group B strains...
  61. pmc Generation and characterization of recombinant influenza A (H1N1) viruses harboring amantadine resistance mutations
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Canada
    Antimicrob Agents Chemother 49:556-9. 2005
    ..These findings could help explain the frequent emergence and transmission of amantadine-resistant influenza viruses during antiviral pressure in the clinical setting...
  62. ncbi request reprint Molecular characterization of a Canadian human parechovirus (HPeV)-3 isolate and its relationship to other HPeVs
    Yacine Abed
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    J Med Virol 77:566-70. 2005
    ..Phylogenetic analysis confirmed the relatedness between Can82853-01 and A308/99 and their divergence from other picornaviruses...
  63. ncbi request reprint Impact of neuraminidase mutations conferring influenza resistance to neuraminidase inhibitors in the N1 and N2 genetic backgrounds
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antivir Ther 11:971-6. 2006
    ..It suggests that certain drug-resistant NA mutants may still be virulent although additional studies using clinical isolates are needed to confirm our results...
  64. ncbi request reprint Influenzavirus infection is a primary cause of febrile respiratory illness in HIV-infected adults, despite vaccination
    Marina B Klein
    Department of Medicine, Division of Infectious Diseases, Royal Victoria Hospital, McGill University Health Centre, Montreal, Quebec, Canada
    Clin Infect Dis 45:234-40. 2007
    ..We performed a surveillance study of outpatients who presented with fever and respiratory symptoms to examine the role of viral pathogens in these patients...
  65. ncbi request reprint Characterization of drug-resistant recombinant influenza A/H1N1 viruses selected in vitro with peramivir and zanamivir
    Mariana Baz
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Que, Canada
    Antiviral Res 74:159-62. 2007
    ..The zanamivir-selected variant had a massive deletion in the region encoding the NA active center and an A200T hemagglutinin mutation. This variant exhibited reduced susceptibility to zanamivir with a drug-dependent phenotype...
  66. doi request reprint Opposite effect of two cytomegalovirus DNA polymerase mutations on replicative capacity and polymerase activity
    Melanie Martin
    Centre Hospitalier Universitaire de Quebec, Quebec City, QC, Canada
    Antivir Ther 15:579-86. 2010
    ..The aim of this study was to characterize the role of two HCMV DNA polymerase mutations (K805Q and T821I) found in a ganciclovir- and foscarnet-resistant clinical isolate from an AIDS patient...
  67. ncbi request reprint Human metapneumovirus infection in adults with community-acquired pneumonia and exacerbation of chronic obstructive pulmonary disease
    M E Hamelin
    Centre Hospitalier Universitaire de Quebec, Sainte Foy, QC, Canada
    Clin Infect Dis 41:498-502. 2005
    ..1%) of 145 adult patients who presented to the emergency department for pneumonia or acute exacerbation of chronic obstructive pulmonary disease during 2 winter/spring seasons in Quebec, Canada...
  68. pmc Human cytomegalovirus resistance to antiviral drugs
    C Gilbert
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Universite Laval, Sainte Foy, Quebec, Canada
    Antimicrob Agents Chemother 49:873-83. 2005
  69. doi request reprint A novel neuraminidase deletion mutation conferring resistance to oseltamivir in clinical influenza A/H3N2 virus
    Yacine Abed
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Québec Centre Hospitalier de l Université Laval and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 199:180-3. 2009
    ..This study highlights the diversity of mechanisms of resistance to oseltamivir in clinical settings, reinforcing the need for novel anti-influenza strategies...
  70. pmc Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses
    Guy Boivin
    Research Center in Infectious Diseases of the CHUQ CHUL, Department of Pediatrics, Laval University, Quebec City, Canada
    J Clin Microbiol 42:45-51. 2004
    ..5 and 98.9% compared to 81.6 and 94.7% for the antigenic test. In conclusion, our multiplex real-time PCR assay combines both rapidity and sensitivity for detecting the most important respiratory viral pathogens in children...
  71. pmc Activity of the oral neuraminidase inhibitor A-322278 against the oseltamivir-resistant H274Y (A/H1N1) influenza virus mutant in mice
    Mariana Baz
    Research Center in Infectious Diseases, CHUQ CHUL, Laval University, Quebec City, Canada
    Antimicrob Agents Chemother 53:791-3. 2009
    ..A-322278 decreased mortality rates and lung virus titers significantly more than oseltamivir in mice infected with the NA H274Y mutant when therapy was started 4 h before or even 48 h after infection...
  72. ncbi request reprint Functional ecology of immature parasitoids
    Jacques Brodeur
    Departement de Phytologie, Universite Laval, Quebec, Canada G1K 7P4
    Annu Rev Entomol 49:27-49. 2004
    ....
  73. ncbi request reprint Quantification of human herpesvirus 8 by real-time PCR in blood fractions of AIDS patients with Kaposi's sarcoma and multicentric Castleman's disease
    Guy Boivin
    Centre Hospitalier Universitaire de Québec CHUQ CHUL and Laval University, Quebec City, Canada
    J Med Virol 68:399-403. 2002
    ..These results suggest that the pathogenesis of the two HHV8-associated diseases (i.e., KS and MCD) might be different, as only the latter was associated with important viremia in our patients...
  74. ncbi request reprint Herpes simplex virus isolates with reduced adefovir susceptibility selected in vivo by foscarnet therapy
    Julie Bestman-Smith
    Centre de Recherche en Infectiologie of the Centre Hospitalier Universitaire de Québec Pavillon CHUL, and Universiteé Laval, Quebec, Canada
    J Med Virol 67:88-91. 2002
    ..6-fold compared to pre-foscarnet or wild-type isolates) suggesting that adefovir-resistant HSV could be selected in vivo by foscarnet therapy...
  75. ncbi request reprint Hydroxyurea enhances the activity of acyclovir and cidofovir against herpes simplex virus type 1 resistant strains harboring mutations in the thymidine kinase and/or the DNA polymerase genes
    Yan Sergerie
    Research Center in Infectious Diseases of the CHUQ CHUL, Quebec City, QC, Canada
    Antiviral Res 77:77-80. 2008
    ..Hydroxyurea, a ribonucleotide reductase inhibitor, can increase the activity of nucleoside/nucleotide analogues against drug-resistant viruses...
  76. ncbi request reprint Syncytium formation and HIV-1 replication are both accentuated by purified influenza and virus-associated neuraminidase
    Jiangfeng Sun
    Centre de Recherche en Infectiologie, Hôpital du Centre Hospitalier Universitaire de L Université Laval, Quebec G1V 4G2, Canada
    J Biol Chem 277:9825-33. 2002
    ..Our results provide a new model allowing the investigation of the potential benefit of using NA inhibitors in the treatment of HIV-1-infected patients suffering from coinfection with NA-bearing pathogens...
  77. pmc Contagious period for pandemic (H1N1) 2009
    Gaston De Serres
    Institut National de Sante Publique du Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 16:783-8. 2010
    ..Self-isolation only until fever abates appears insufficient to limit transmission. Self-isolation for a week may be more effective, although some case-patients still would shed infectious virus...
  78. ncbi request reprint Characterization of 2 influenza A(H3N2) clinical isolates with reduced susceptibility to neuraminidase inhibitors due to mutations in the hemagglutinin gene
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    J Infect Dis 186:1074-80. 2002
    ..Our study shows the importance of the HA1 229 residue in virus binding to MDCK cells and confirms the unreliability of cell-based assays in predicting the in vivo susceptibility of HA variants to NAIs...
  79. pmc Oseltamivir-resistant pandemic A/H1N1 virus is as virulent as its wild-type counterpart in mice and ferrets
    Marie Eve Hamelin
    CHUQ CHUL Research Center in Infectious Diseases and Laval University, Quebec City, Quebec, Canada
    PLoS Pathog 6:e1001015. 2010
    ..Furthermore, the H274Y mutant strain was transmitted to ferrets. In conclusion, viral fitness of the H274Y pH1N1 isolate is not substantially altered and has the potential to induce severe disease and to disseminate...
  80. ncbi request reprint Human metapneumovirus: a ubiquitous and long-standing respiratory pathogen
    Marie Eve Hamelin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Canada
    Pediatr Infect Dis J 24:S203-7. 2005
    ..Human metapneumovirus (hMPV) is a recently described human pathogen first identified in respiratory specimens of young children suffering from respiratory syndromes ranging from mild to severe...
  81. ncbi request reprint Human metapneumovirus
    Céline Deffrasnes
    Department of Medical Biology, Faculty of Medicine, Laval University, Quebec, Canada
    Semin Respir Crit Care Med 28:213-21. 2007
    ..No commercial treatments are yet available for hMPV, although ribavirin has shown activity both in vitro and in animal models. Live attenuated vaccines produced by reverse genetics have also shown good efficacy in animals...
  82. ncbi request reprint Characterization of human cytomegalovirus (HCMV) UL97 mutations found in a valganciclovir/oral ganciclovir prophylactic trial by use of a bacterial artificial chromosome containing the HCMV genome
    Melanie Martin
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Canada
    J Infect Dis 194:579-83. 2006
    ..All recombinant viruses containing these unknown mutations were found to be susceptible to ganciclovir...
  83. pmc Effect of ribavirin and glucocorticoid treatment in a mouse model of human metapneumovirus infection
    Marie Eve Hamelin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Quebec, Sainte Foy, Quebec, Canada
    Antimicrob Agents Chemother 50:774-7. 2006
    ..Ribavirin significantly decreased both hMPV replication in lungs (by 5 log10) and global pulmonary inflammation on day 5 postinfection, whereas glucocorticoids reduced only alveolar and interstitial inflammation, compared to controls...
  84. doi request reprint Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
    Irena Kukavica-Ibrulj
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    J Clin Virol 44:88-90. 2009
    ..Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups...
  85. pmc Infection with human metapneumovirus predisposes mice to severe pneumococcal pneumonia
    Irena Kukavica-Ibrulj
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Quebec City, Quebec, Canada
    J Virol 83:1341-9. 2009
    ..Prior infection with either hMPV or influenza A virus predisposes mice to severe pneumococcus infection...
  86. pmc Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children
    Frederic Raymond
    Infectious Disease Research Center of the CHUQ CHUL and Laval University, Sainte Foy, Quebec, Canada
    J Clin Microbiol 47:743-50. 2009
    ..The two methods yielded concordant results for 94.1% of specimens. These tests allowed a thorough etiological assessment of respiratory viruses infecting children in hospital settings and would assist public health interventions...
  87. ncbi request reprint Enhanced lung disease and Th2 response following human metapneumovirus infection in mice immunized with the inactivated virus
    Marie Eve Hamelin
    Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, 2705 Blvd Laurier, Quebec City, QC G1V 4G2, Canada
    J Gen Virol 88:3391-400. 2007
    ..Our data demonstrate the risks associated with immunization using inactivated hMPV in this animal model and that this aberrant response should be considered in the development of hMPV vaccines...
  88. ncbi request reprint Activity of the neuraminidase inhibitor A-315675 against oseltamivir-resistant influenza neuraminidases of N1 and N2 subtypes
    Yacine Abed
    Research Center in Infectious Diseases of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Antiviral Res 77:163-6. 2008
    ..These results confirm the potent inhibitory effect of A-315675 against oseltamivir-resistant influenza viruses of the N1 and N2 subtypes and support the clinical development of its bioavailable prodrug A-322278...
  89. ncbi request reprint Three-dimensional modeling of cytomegalovirus DNA polymerase and preliminary analysis of drug resistance
    Rong Shi
    Research Center in Molecular Endocrinology, Centre Hospitalier Universitaire de Québec CHUL hospital and Laval University, Quebec City, Canada
    Proteins 64:301-7. 2006
    ..These results constitute a first step towards facilitating our understanding of drug resistance mechanisms for CMV and the interpretation of novel viral mutations...
  90. ncbi request reprint Estradiol is required for a proper immune response to bacterial and viral pathogens in the female brain
    Genevieve Soucy
    Laboratory of Molecular Endocrinology, Centre Hospitalier de l Universite Laval Research Center, Quebec City, Quebec, Canada
    J Immunol 174:6391-8. 2005
    ..This transfer is deviant in mice lacking E(2), which allows pathogens to hide from immune surveillance and exacerbates neuronal damages during viral encephalitis...
  91. ncbi request reprint Geographical origin of an introduced pest species, Delia radicum (Diptera: anthomyiidae), determined by RAPD analysis and egg micromorphology
    D G Biron
    Departement des Sciences Biologiques, Universite du Quebec a Montreal, CP 8888, Succursale Centre Ville, Montreal, Quebec, Canada, H3C 3P8
    Bull Entomol Res 90:23-32. 2000
    ..radicum into North America, probably via the north-eastern coast (New York area) from north-western Europe. The implications of this study in assisting selection of natural enemies of this important agricultural pest are discussed...
  92. pmc Highly reliable heterologous system for evaluating resistance of clinical herpes simplex virus isolates to nucleoside analogues
    J Bestman-Smith
    Centre de Recherche en Infectiologie, , and Department of Medical Biology, , Sainte-Foy, , Canada
    J Virol 75:3105-10. 2001
    ..The expression of the HSV TK gene in Leishmania provides an easy, reliable, and sensitive assay for evaluating HSV susceptibility to nucleoside analogues and for assessing the role of specific viral TK mutations...
  93. pmc Recombinant phenotyping of cytomegalovirus UL54 mutations that emerged during cell passages in the presence of either ganciclovir or foscarnet
    Christian Gilbert
    Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Quebec and Laval University, Quebec City, Quebec, Canada
    Antimicrob Agents Chemother 55:4019-27. 2011
    ....
  94. pmc The I222V neuraminidase mutation has a compensatory role in replication of an oseltamivir-resistant influenza virus A/H3N2 E119V mutant
    Philippe Simon
    Centre de Recherche en Infectiologie, Universite Laval, 2705 Boul Laurier, Quebec City, Quebec G1V 4G2, Canada
    J Clin Microbiol 49:715-7. 2011
    ..Based on plaque size, yield assays, and NA activity, the impaired viral fitness of the E119V mutant was partially restored by the I222V NA mutation...
  95. pmc Resistance of herpes simplex viruses to nucleoside analogues: mechanisms, prevalence, and management
    Jocelyne Piret
    Centre de Recherche en Infectiologie, Universite Laval, Quebec, Quebec, Canada
    Antimicrob Agents Chemother 55:459-72. 2011
    ..There is a need to develop new antiherpetic compounds with different mechanisms of action...
  96. pmc Evaluation of serological diagnostic methods for the 2009 pandemic influenza A (H1N1) virus
    Jesse Papenburg
    Infectious Disease Research Center of the CHUQ CHUL and Laval University, Quebec City, Quebec, Canada
    Clin Vaccine Immunol 18:520-2. 2011
    ..Compared to hemagglutination inhibition, microneutralization demonstrated a higher level of concordance with RT-PCR (kappa = 0.69 versus kappa = 0.60) and greater sensitivity (83% versus 71%; P = 0.016)...
  97. pmc Viable but nonculturable Vibrio cholerae O1 in the aquatic environment of Argentina
    Norma Binsztein
    Departamento de Bacteriologia, Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr Carlos G Malbran, Ministerio de Salud, Ciudad de Buenos Aires, Argentina
    Appl Environ Microbiol 70:7481-6. 2004
    ..cholerae can revert to the pathogenic, transmissible state. We concluded that V. cholerae O1 is a resident of Argentinean waters, as has been shown to be the case in other geographic regions of the world...