Beny Spira

Summary

Country: Brazil

Publications

  1. pmc The constancy of global regulation across a species: the concentrations of ppGpp and RpoS are strain-specific in Escherichia coli
    Thomas Ferenci
    School of Molecular and Microbial Biosciences, The University of Sydney, NSW, Australia
    BMC Microbiol 11:62. 2011
  2. ncbi Differentially expressed genes in the liver of thioacetamide treated rats
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes 1374, São Paulo SP CEP 05508 900, Brazil
    Comp Biochem Physiol C Toxicol Pharmacol 135:129-35. 2003
  3. ncbi Alkaline phosphatase as a reporter of sigma(S) levels and rpoS polymorphisms in different E. coli strains
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, CEP 05508 900, Sao Paulo, SP, Brazil
    Arch Microbiol 189:43-7. 2008
  4. pmc The uncertain consequences of transferring bacterial strains between laboratories - rpoS instability as an example
    Beny Spira
    Departamento de Microbiologia, Universidade de Sao Paulo, Sao Paulo, Brazil
    BMC Microbiol 11:248. 2011
  5. doi Alternative promoters in the pst operon of Escherichia coli
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, Sao Paulo, SP 05508 900, Brazil
    Mol Genet Genomics 284:489-98. 2010
  6. ncbi The effect of IHF on sigmaS selectivity of the phoA and pst promoters of Escherichia coli
    Natalia Pasternak Taschner
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes 1374, CEP 05508 900, Sao Paulo, Brazil
    Arch Microbiol 185:234-7. 2006
  7. ncbi A differential effect of sigmaS on the expression of the PHO regulon genes of Escherichia coli
    Natalia Pasternak Taschner
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Professor Lineu Prestes, 1374, São Paulo SP CEP 05508 900, Brazil
    Microbiology 150:2985-92. 2004
  8. doi Stability of the pstS transcript of Escherichia coli
    Meire Aguena
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, Sao Paulo, SP, CEP 05508 900, Brazil
    Arch Microbiol 191:105-12. 2009
  9. doi Strain variation in ppGpp concentration and RpoS levels in laboratory strains of Escherichia coli K-12
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo SP, Brazil
    Microbiology 154:2887-95. 2008
  10. doi Transcriptional processing of the pst operon of Escherichia coli
    Meire Aguena
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, Sao Paulo, SP CEP 05508 900, Brazil
    Curr Microbiol 58:264-7. 2009

Collaborators

Detail Information

Publications15

  1. pmc The constancy of global regulation across a species: the concentrations of ppGpp and RpoS are strain-specific in Escherichia coli
    Thomas Ferenci
    School of Molecular and Microbial Biosciences, The University of Sydney, NSW, Australia
    BMC Microbiol 11:62. 2011
    ..To find out the extent of strain variation we measured the level of RpoS and ppGpp using 31 E. coli strains from the ECOR collection and one reference K-12 strain...
  2. ncbi Differentially expressed genes in the liver of thioacetamide treated rats
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes 1374, São Paulo SP CEP 05508 900, Brazil
    Comp Biochem Physiol C Toxicol Pharmacol 135:129-35. 2003
    ..It is proposed that since the product of TAP is involved in mRNA transport, thioacetamide inhibition of TAP expression might, at least partially, explain the thioacetamide-induced swelling of the nucleolus...
  3. ncbi Alkaline phosphatase as a reporter of sigma(S) levels and rpoS polymorphisms in different E. coli strains
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, CEP 05508 900, Sao Paulo, SP, Brazil
    Arch Microbiol 189:43-7. 2008
    ..Consequently, a simple and non-destructive AP assay can be employed to differentiate between strains expressing different levels of sigma(S) on agar plates...
  4. pmc The uncertain consequences of transferring bacterial strains between laboratories - rpoS instability as an example
    Beny Spira
    Departamento de Microbiologia, Universidade de Sao Paulo, Sao Paulo, Brazil
    BMC Microbiol 11:248. 2011
    ..Under nutrient limiting conditions mutations in rpoS or in genes that regulate its expression tend to accumulate. Here, we investigate whether short-term storage and mailing of cultures in stabs results in genetic heterogeneity...
  5. doi Alternative promoters in the pst operon of Escherichia coli
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, Sao Paulo, SP 05508 900, Brazil
    Mol Genet Genomics 284:489-98. 2010
    ..Plasmids carrying the pst internal genes partially complement chromosomal mutations in their corresponding genes, indicating that they are translated into functional proteins...
  6. ncbi The effect of IHF on sigmaS selectivity of the phoA and pst promoters of Escherichia coli
    Natalia Pasternak Taschner
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes 1374, CEP 05508 900, Sao Paulo, Brazil
    Arch Microbiol 185:234-7. 2006
    ..Introduction of a -13C residue in the promoter region of phoA, another PHO regulon gene that is not directly affected by IHF, did not affect its exclusive transcription initiation by Esigma (D)...
  7. ncbi A differential effect of sigmaS on the expression of the PHO regulon genes of Escherichia coli
    Natalia Pasternak Taschner
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Professor Lineu Prestes, 1374, São Paulo SP CEP 05508 900, Brazil
    Microbiology 150:2985-92. 2004
    ..The interpretation of these observations is based on the competition between sigma(S) and sigma(D) for the binding to the core RNA polymerase...
  8. doi Stability of the pstS transcript of Escherichia coli
    Meire Aguena
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, Sao Paulo, SP, CEP 05508 900, Brazil
    Arch Microbiol 191:105-12. 2009
    ..The presence of the REP sequence also results in an increased level of PstS proteins. However, the surplus level of PstS proteins produced in the presence of REP does not contribute to the repressive role of Pst in PHO expression...
  9. doi Strain variation in ppGpp concentration and RpoS levels in laboratory strains of Escherichia coli K-12
    Beny Spira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo SP, Brazil
    Microbiology 154:2887-95. 2008
    ..coli...
  10. doi Transcriptional processing of the pst operon of Escherichia coli
    Meire Aguena
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, Sao Paulo, SP CEP 05508 900, Brazil
    Curr Microbiol 58:264-7. 2009
    ..Other ribonucleases, such as RNase III and MazF, do not play a role in pst mRNA processing. RNase E is thus at least partially responsible for processing the pst primary transcript...
  11. ncbi Thioacetamide differentially affects the expression and activity of glutathione-S-transferase in the liver of Wistar rats
    Murilo Tadeu Domingues Bueno
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
    Hum Exp Toxicol 23:431-7. 2004
    ..Differences in the basal level of class alpha mRNA were observed in rats from at least three different sources, while class mu mRNA level was distinct in two groups of animals...
  12. ncbi RT-PCR of long prokaryotic operon transcripts without DNase treatment
    Meire Aguena
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes 1374, Sao Paulo, SP 05508 900, Brazil
    J Microbiol Methods 55:419-23. 2003
    ..To avoid amplification of genomic DNA, the hybrid-primer molecules that were not incorporated into the first-strand cDNA were removed by RNase H treatment followed by ultrafiltration...
  13. doi The pst operon of enteropathogenic Escherichia coli enhances bacterial adherence to epithelial cells
    Gerson Moura Ferreira
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Prof Lineu Prestes, 1374, São Paulo SP CEP 05508 900, Brazil
    Microbiology 154:2025-36. 2008
    ..Overall, the data presented here demonstrate that the pst operon of EPEC plays a positive role in the bacterial adherence mechanism by increasing the expression of perA and perC and consequently the transcription of bfp and eae...
  14. ncbi Variation in stress responses within a bacterial species and the indirect costs of stress resistance
    Thomas Ferenci
    School of Molecular and Microbial Biosciences G08, The University of Sydney, NSW 2006, Australia
    Ann N Y Acad Sci 1113:105-13. 2007
    ..The intimate association of RpoS/ppGpp with stress resistance and SPANC balancing influences numerous cellular processes and bacterial properties, including virulence...
  15. pmc relA enhances the adherence of enteropathogenic Escherichia coli
    Beny Spira
    Departamento de Microbiologia, Instituto de Cêincias Biomédicas, Universidade de Sao Paulo, Sao Paulo SP, Brazil
    PLoS ONE 9:e91703. 2014
    ..Bacterial adherence, BFP and intimin synthesis and perABC expression are restored upon complementation with the wild-type relA allele. ..