Celio L Silva

Summary

Country: Brazil

Publications

  1. pmc DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes
    Rubens R Santos
    University of Sao Paulo, Ribeirao Preto Medical School, Department of Biochemistry and Immunology, Ribeirao Preto, Sao Paulo, Brazil
    J Immune Based Ther Vaccines 7:4. 2009
  2. pmc Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock protein
    Larissa Lumi Watanabe Ishikawa
    Department of Microbiology and Immunology, Biosciences Institute, , Botucatu, , 18618 000, Brazil
    Genet Vaccines Ther 7:11. 2009
  3. ncbi request reprint DNA encoding individual mycobacterial antigens protects mice against tuberculosis
    C L Silva
    Departamento de Parasitologia, Microbiologia e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brasil
    Braz J Med Biol Res 32:231-4. 1999
  4. ncbi request reprint Cytotoxic T cells and mycobacteria
    C L Silva
    Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil
    FEMS Microbiol Lett 197:11-8. 2001
  5. ncbi request reprint Vaccines of the future: from rational design to clinical development. 17-19 October 2001, Paris, France
    Celio L Silva
    REDE TB Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, 14049 900 Avenida dos Bandeirantes 3900, Riberão Preto, Sao Paulo, Brazil
    Expert Opin Biol Ther 2:219-22. 2002
  6. pmc Blockade of endogenous leukotrienes exacerbates pulmonary histoplasmosis
    Alexandra I Medeiros
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, São Paulo 14040 903, Brazil
    Infect Immun 72:1637-44. 2004
  7. pmc Helminth coinfection does not affect therapeutic effect of a DNA vaccine in mice harboring tuberculosis
    Fabiani G Frantz
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
    PLoS Negl Trop Dis 4:e700. 2010
  8. ncbi request reprint Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis
    Patrícia R M Souza
    Universidade de Sao Paulo, Núcleo de Pesquisas em Tuberculose, Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, FMRP USP, 14049 900 Ribeirao Preto, SP, Brasil
    Expert Opin Biol Ther 8:1255-64. 2008
  9. ncbi request reprint Immunomodulation and protection induced by DNA-hsp65 vaccination in an animal model of arthritis
    Rubens R Santos-Junior
    Centro de Pesquisas em Tuberculose, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, 14049 900, Sao Paulo, Brazil
    Hum Gene Ther 16:1338-45. 2005
  10. doi request reprint Modulation of angiogenic factor VEGF by DNA-hsp65 vaccination in a murine CNS tuberculosis model
    Fabiola C R Zucchi
    Department of Neuroscience, University of São Paulo at Ribeirão Preto School of Medicine, Ribeirao Preto, SP, Brazil
    Tuberculosis (Edinb) 93:373-80. 2013

Collaborators

Detail Information

Publications40

  1. pmc DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes
    Rubens R Santos
    University of Sao Paulo, Ribeirao Preto Medical School, Department of Biochemistry and Immunology, Ribeirao Preto, Sao Paulo, Brazil
    J Immune Based Ther Vaccines 7:4. 2009
    ..abstract:..
  2. pmc Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock protein
    Larissa Lumi Watanabe Ishikawa
    Department of Microbiology and Immunology, Biosciences Institute, , Botucatu, , 18618 000, Brazil
    Genet Vaccines Ther 7:11. 2009
    ..CONCLUSION: 20% restriction in food intake deeply compromised humoral immunity induced by a genetic vaccine, alerting, therefore, for the relevance of the nutritional condition in vaccination programs based on these kinds of constructs...
  3. ncbi request reprint DNA encoding individual mycobacterial antigens protects mice against tuberculosis
    C L Silva
    Departamento de Parasitologia, Microbiologia e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brasil
    Braz J Med Biol Res 32:231-4. 1999
    ..Our most recent studies indicate that DNA vaccination is an effective way to establish long-lasting cytotoxic T cell memory and protection against tuberculosis...
  4. ncbi request reprint Cytotoxic T cells and mycobacteria
    C L Silva
    Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil
    FEMS Microbiol Lett 197:11-8. 2001
    ..Based on recent evidence, we express here the view that cytotoxic T lymphocytes also make an important contribution and suggest that DNA vaccines might be a good way to enhance this...
  5. ncbi request reprint Vaccines of the future: from rational design to clinical development. 17-19 October 2001, Paris, France
    Celio L Silva
    REDE TB Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, 14049 900 Avenida dos Bandeirantes 3900, Riberão Preto, Sao Paulo, Brazil
    Expert Opin Biol Ther 2:219-22. 2002
    ..Specific issues related to the development of therapeutic vaccines directed against diseases such as cancer or chronic infectious disease were presented...
  6. pmc Blockade of endogenous leukotrienes exacerbates pulmonary histoplasmosis
    Alexandra I Medeiros
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, São Paulo 14040 903, Brazil
    Infect Immun 72:1637-44. 2004
    ..This finding may enable us to alter the course of the immune response and inflammation caused by histoplasmosis. The data from the present study suggest an important new strategy for immunologic or drug intervention in human patients...
  7. pmc Helminth coinfection does not affect therapeutic effect of a DNA vaccine in mice harboring tuberculosis
    Fabiani G Frantz
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
    PLoS Negl Trop Dis 4:e700. 2010
    ..leprae (DNAhsp65) has been used in therapy during experimental tuberculosis. This study focused on the impact of the co-existence of worms and TB on the therapeutic effects of DNAhsp65...
  8. ncbi request reprint Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis
    Patrícia R M Souza
    Universidade de Sao Paulo, Núcleo de Pesquisas em Tuberculose, Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, FMRP USP, 14049 900 Ribeirao Preto, SP, Brasil
    Expert Opin Biol Ther 8:1255-64. 2008
    ..Nonetheless, there are substantial data indicating that BCG, the only available anti-TB vaccine for clinical use, provides other important beneficial effects in immunized infants...
  9. ncbi request reprint Immunomodulation and protection induced by DNA-hsp65 vaccination in an animal model of arthritis
    Rubens R Santos-Junior
    Centro de Pesquisas em Tuberculose, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, 14049 900, Sao Paulo, Brazil
    Hum Gene Ther 16:1338-45. 2005
    ..Our results strongly suggest that DNA-hsp65 has no arthritogenic potential and is actually protective against experimentally induced arthritis in mice...
  10. doi request reprint Modulation of angiogenic factor VEGF by DNA-hsp65 vaccination in a murine CNS tuberculosis model
    Fabiola C R Zucchi
    Department of Neuroscience, University of São Paulo at Ribeirão Preto School of Medicine, Ribeirao Preto, SP, Brazil
    Tuberculosis (Edinb) 93:373-80. 2013
    ..The DNA-hsp65 treatment blocked the expression of VEGF in mice TBM. Therefore, our murine model indicated the VEGF participation in the physiopathology of CNS-TB and the potential prevention of the DNA-hsp65 in the disease progression...
  11. pmc Intranasal vaccination with messenger RNA as a new approach in gene therapy: use against tuberculosis
    Julio C C Lorenzi
    Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Brazil
    BMC Biotechnol 10:77. 2010
    ..The use of mRNA as strategy for the stimulation of the immune system has been used mainly in current strategies for the cancer treatment but until now no one tested this molecule as vaccine for infectious disease...
  12. ncbi request reprint Comparison of different delivery systems of vaccination for the induction of protection against tuberculosis in mice
    K M Lima
    Department of Biochemistry and Immunology, , , Av. Bandeirantes 3900, 14049-900, SP, Ribeirao Preto, Brazil
    Vaccine 19:3518-25. 2001
    ....
  13. pmc Protection against tuberculosis by a single intranasal administration of DNA-hsp65 vaccine complexed with cationic liposomes
    Rogério S Rosada
    Núcleo de Pesquisas em Tuberculose, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo
    BMC Immunol 9:38. 2008
    ..Our technique consists of a single-dose, genetic vaccine formulation of DNA-hsp65 complexed with cationic liposomes and administered intranasally...
  14. ncbi request reprint Vaccine adjuvant: it makes the difference
    Karla M Lima
    Center for Tuberculosis Research, School of Medicine of Ribeirao Preto, Instituto do Milênio REDE TB, University of Sao Paulo, Avenue Bandeirantes 3900, Ribeirao Preto, Sao Paulo 14049 900, Brazil
    Vaccine 22:2374-9. 2004
    ..Thus, the same antigen might or not protect mice from challenge with Mycobacterium tuberculosis, depending on the formulation. These data suggest that, despite the name, the adjuvant plays a fundamental role on the vaccination process...
  15. ncbi request reprint TLR2-dependent mast cell activation contributes to the control of Mycobacterium tuberculosis infection
    Daniela Carlos
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, SP 14 040 903, Brazil
    Microbes Infect 11:770-8. 2009
    ..Our results suggest that activation of MCs via TLR2 is required to compensate the defect in protective immunity and inability of TLR2(-/-) mice to control MTB infection...
  16. doi request reprint Recent advances in DNA vaccines for autoimmune diseases
    Celio L Silva
    The Centre for Tuberculosis Research, Department of Biochemistry and Immunology, Medicine School of Ribeirao Preto, University of Sao Paulo, CEP 14049 900, Brazil
    Expert Rev Vaccines 8:239-52. 2009
    ..Our experience with a genetic vaccine containing the heat-shock protein gene (hsp65) from mycobacteria is also described...
  17. ncbi request reprint Genetic aspects and microenvironment affect expression of CD18 and VLA-4 in experimental tuberculosis
    V L D Bonato
    REDE TB, Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil
    Scand J Immunol 56:185-94. 2002
    ..Thus, the up- or downmodulation of these adhesion receptors is probably associated with differential recruitment of T cells at the site of infection, which may or may not mediate protection in experimental tuberculosis...
  18. pmc Low-dose plasmid DNA treatment increases plasma vasopressin and regulates blood pressure in experimental endotoxemia
    Thiago Malardo
    Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto SP 14049 900, Brazil
    BMC Immunol 13:59. 2012
    ..Although plasmid DNA encoding an antigen from pathogens or tumor cells has been widely studied as vaccine, the use of plasmid vector (without insert) as therapeutic agent requires further investigation...
  19. doi request reprint Antimycobacterial activity of 2-phenoxy-1-phenylethanone, a synthetic analogue of neolignan, entrapped in polymeric microparticles
    Paulo R R Minarini
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Drug Dev Ind Pharm 38:259-63. 2012
    ..Chemotherapeutic potential of LS-2 entrapped in microparticles was comparable to control groups. These findings are encouraging and indicate that LS-2-loaded microparticles are a potential alternative to conventional chemotherapy of TB...
  20. pmc B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
    Luciana P Almeida
    Department of Biochemistry and Immunology, Medical School of Ribeirao Preto, University of Sao Paulo, Brazil
    Genet Vaccines Ther 9:5. 2011
    ..abstract:..
  21. ncbi request reprint Th1 polarized response induced by intramuscular DNA-HSP65 immunization is preserved in experimental atherosclerosis
    D M Fonseca
    Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brasil
    Braz J Med Biol Res 40:1495-504. 2007
    ..These results indicate that experimentally induced atherosclerosis did not affect the ability of DNA-HSP65 to induce a predominant Th1 response that is potentially protective against tuberculosis...
  22. ncbi request reprint The Mycobacterium leprae hsp65 displays proteolytic activity. Mutagenesis studies indicate that the M. leprae hsp65 proteolytic activity is catalytically related to the HslVU protease
    Fernanda C V Portaro
    Center for Applied Toxinology, Butantan Institute, CAT CEPID, Rua Murajuba 125, Sao Paulo, SP 05467 010, Brazil
    Biochemistry 41:7400-6. 2002
    ..leprae hsp65. The possible pathophysiological implications of the proteolytic activity of the M. leprae hsp65 are now under investigation in our laboratory...
  23. pmc Therapeutic efficacy of Cintredekin Besudotox (IL13-PE38QQR) in murine lung fibrosis is unaffected by immunity to Pseudomonas aeruginosa exotoxin A
    Rogério S Rosada
    Núcleo de Pesquisa em Tuberculose, Departamento de Bioquimica e Imunologia, Universidade de Sao Paulo, Sao Paulo, Brazil
    PLoS ONE 5:e8721. 2010
    ..The aim of the present study was to determine whether the presence of an immune response to P. aeruginosa and/or its exotoxin A (PE) would diminish the anti-fibrotic properties of IL13-PE...
  24. ncbi request reprint pcDNA-IL-12 vaccination blocks eosinophilic inflammation but not airway hyperresponsiveness following murine Toxocara canis infection
    Adriana Malheiro
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av Café S N, 14040 903 Ribeirao Preto, SP, Brazil
    Vaccine 26:305-15. 2008
    ..canis infection, suggesting their possible use in further combined therapeutic interventions...
  25. pmc Endocytosis of DNA-Hsp65 alters the pH of the late endosome/lysosome and interferes with antigen presentation
    Ana Paula F Trombone
    Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil
    PLoS ONE 2:e923. 2007
    ....
  26. pmc Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: activation of CD8+ cells, interferon-gamma recovery and reduction of lung injury
    V L D Bonato
    TB Network, Department of Biochemistry and Immunology, Ribeirao Preto School of Medicine, University of Sao Paulo, Brazil
    Immunology 113:130-8. 2004
    ..Treatment with pcDNA3 vector also induced an immune stimulatory effect. However, only infected pHSP65-treated mice were able to produce significant levels of interferon-gamma and to restrict the growth of bacilli...
  27. pmc Identification and characterization of murine cytotoxic T cells that kill Mycobacterium tuberculosis
    C L Silva
    Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil
    Infect Immun 68:3269-74. 2000
    ..Granule-mediated cytotoxicity thus may have a key role in protection against tuberculosis by delivering mycobactericidal granule contents...
  28. ncbi request reprint Efficacy of DNA-hsp65 vaccination for tuberculosis varies with method of DNA introduction in vivo
    K M Lima
    Instituto do Milênio REDE TB, Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, 14049 900 Ribeirao Preto, SP, Brazil
    Vaccine 22:49-56. 2003
    ..However, in contrast to intramuscular immunization, which was protective in these studies, gene gun immunization did not protect BALB/c mice against challenge infection...
  29. ncbi request reprint Downmodulation of CD18 and CD86 on macrophages and VLA-4 on lymphocytes in experimental tuberculosis
    V L Bonato
    Centre for Tuberculosis Research, Department of Biochemistry and Immunology, , , Brazil
    Scand J Immunol 54:564-73. 2001
    ..The interplay among IL-12, IFN-gamma and IL-10 in vivo and the downmodulation of cell-surface receptors during the infection at the inflammatory site may contribute to the explanation of the maintenance of infection...
  30. ncbi request reprint Single dose of a vaccine based on DNA encoding mycobacterial hsp65 protein plus TDM-loaded PLGA microspheres protects mice against a virulent strain of Mycobacterium tuberculosis
    K M Lima
    Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil
    Gene Ther 10:678-85. 2003
    ..Thus, this combination of DNA vaccine and adjuvants with immunomodulatory and carrier properties holds the potential for an improved vaccine against tuberculosis...
  31. doi request reprint Phase I trial of DNA-hsp65 immunotherapy for advanced squamous cell carcinoma of the head and neck
    P Michaluart
    HSP65 Clinical Trial Group, University of Sao Paulo, Sao Paulo, Brazil
    Cancer Gene Ther 15:676-84. 2008
    ..Further studies in a larger number of patients are needed to confirm the efficacy of this novel strategy...
  32. pmc Histamine plays an essential regulatory role in lung inflammation and protective immunity in the acute phase of Mycobacterium tuberculosis infection
    D Carlos
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Infect Immun 77:5359-68. 2009
    ..tuberculosis infection may play a regulatory role not only by enhancing the pulmonary neutrophilia and production of IL-6 and TNF-alpha but also by impairing the protective Th1 response, which ultimately restricts mycobacterial growth...
  33. ncbi request reprint Immunotherapy with plasmid DNA encoding mycobacterial hsp65 in association with chemotherapy is a more rapid and efficient form of treatment for tuberculosis in mice
    C L Silva
    REDE TB, Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Gene Ther 12:281-7. 2005
    ....
  34. pmc Role of trehalose dimycolate in recruitment of cells and modulation of production of cytokines and NO in tuberculosis
    V M Lima
    Department of Biochemistry and Immunology, , , SP, Brazil
    Infect Immun 69:5305-12. 2001
    ..In addition, our findings are important for a better understanding of the immunostimulatory activity of TDM and its possible use as an adjuvant in experiments using DNA vaccine or gene therapy against tuberculosis...
  35. pmc Inhibition of leukotriene biosynthesis abrogates the host control of Mycobacterium tuberculosis
    Camila M Peres
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo USP, Av do Cafe S N, Ribeirao Preto, São Paulo 14040 903, Brazil
    Microbes Infect 9:483-9. 2007
    ....
  36. ncbi request reprint Mycobacterium tuberculosis expressing phospholipase C subverts PGE2 synthesis and induces necrosis in alveolar macrophages
    Patricia A Assis
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av Café, S N, Ribeirao Preto, SP 14040 903, Brazil
    BMC Microbiol 14:128. 2014
    ..It has been reported that induction of cell necrosis by virulent Mtb is coordinated by subversion of PGE2, an essential factor in cell membrane protection...
  37. doi request reprint The contribution of a murine CNS-TB model for the understanding of the host-pathogen interactions in the formation of granulomas
    Fabiola C R Zucchi
    Department of Neuroscience, University of São Paulo at Ribeirão Preto School of Medicine, Ribeirao Preto, SP, Brazil
    J Neurosci Methods 206:88-93. 2012
    ....
  38. doi request reprint Characterization and in vitro activities of cell-free antigens from Histoplasma capsulatum-loaded biodegradable microspheres
    Daiane F dos Santos
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av do Cafe S N, 14040 903 Ribeirao Preto, Sao Paulo, Brazil
    Eur J Pharm Sci 38:548-55. 2009
    ..CFAgs-loaded MS present potential to be used as vaccine in order to confer protection against H. capsulatum infection...
  39. ncbi request reprint Gr-1+ cells play an essential role in an experimental model of disseminated histoplasmosis
    Anderson Sá-Nunes
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av do Café s no, Ribeirao Preto, São Paulo 14040 903, Brazil
    Microbes Infect 9:1393-401. 2007
    ..This study improves the understanding of the role of Gr-1(+) cells on the protective immunity in histoplasmosis...
  40. ncbi request reprint Cytotoxicity, antitumoral and antimycobacterial activity of tetrazole and oxadiazole derivatives
    A O De Souza
    Department of Biochemistry and Immunology, School of Medicine of Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, S P, Brazil
    Pharmazie 60:396-7. 2005
    ..Structure Activity Relationship (SAR) analysis was performed using Principal Component Analysis (PCA) to determine the relationship between these compounds and their biological activities...