Affiliation: Instituto Butantan
- Effect of medium composition on the production of tetanus toxin by Clostridium tetaniFernando Fratelli
Division of Technology and Production Development of Institute Butantan, Av Vital Brazil, 1500, 05504 900 São Paulo SP, Brazil
Biotechnol Prog 21:756-61. 2005..The highest final average yield of tetanus toxin (72 Lf/mL), achieved at G0= 9.7 g/L and NZ0= 43.5 g/L, was 80% higher than that obtained with standard cultivations (G0= 8.0 g/L and NZ0= 25.0 g/L)...
- Fed-batch production of tetanus toxin by Clostridium tetaniFernando Fratelli
Div of Technology and Production Development of Institute Butantan, Av Vital Brasil, 1500, 05503 900 Sao Paulo, SP, Brazil
Biotechnol Prog 26:88-92. 2010..The results of this work promise to substantially improve the present production of tetanus toxin and may be adopted for human vaccine production after detoxification and purification...
- Development of an operational synaptobrevin-based fluorescent substrate for tetanus neurotoxin quantificationElen A Perpetuo
Laboratory of Biochemistry and Biophysics, Center of Applied Toxinology, CEPID, Av Vital Brasil, 1500 Butantan, 05503 900 Sao Paulo, SP, Brazil
Biotechnol Appl Biochem 36:155-61. 2002..It is a good alternative to some of the existing methods such as flocculation assay, and it can replace, under some conditions, the biological assays (minimal mortal dose)...