Research Topics
Genomes and GenesSpecies | Evanguedes KalapothakisSummaryAffiliation: Belo Horizonte Country: Brazil Publications
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Detail Information
Publications
Screening of expression libraries using ELISA: identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venomE Kalapothakis
Divisao de Biologia Celular, Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, MG 31 270901, Belo Horizonte, Brazil
Toxicon 39:679-85. 2001..The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects...- Brown spider dermonecrotic toxin directly induces nephrotoxicityOlga Meiri Chaim
Department of Cell Biology, Federal University of Paran, , 81531-990, Curitiba, Paran, Brazil
Toxicol Appl Pharmacol 211:64-77. 2006.... - Evaluation of Brazilian biotechnology patent activity from 1975 to 2010F Dias
Departamento de Biologia Geral, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Recent Pat DNA Gene Seq 6:145-59. 2012..The first biotechnology patent applications via PCT were submitted by Brazilians in 1997, with 3 from UFMG (university), 2 from individuals, and 1 from EMBRAPA (research institute)... - The Loxtox protein family in Loxosceles intermedia (Mello-Leitão) venomE Kalapothakis
Departamento de Biologia Geral Instituto de Ciências Biológicas Universidade Federal de Minas Gerais, CEP 31270901 Belo Horizonte, MG, Brazil
Toxicon 50:938-46. 2007..The finding of several isoforms of Loxtox in the venom of this spider may reflect an evolutionary adaptation for different prey types and reinforces the idea of an efficient mutational mechanism in the venom gland of spiders... - Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venomEvanguedes Kalapothakis
Departamento de Farmacologia, Divisao de Biologia Celular, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Belo Horizonte, MG, CEP 31270901, Brazil
Toxicon 40:1691-9. 2002..recLiD1 was strongly recognised by anti-dermonecrotic antibodies and was also able to generate reactive antibodies against native dermonecrotic proteins isolated from the venom of L. intermedia... - Protection against dermonecrotic and lethal activities of Loxosceles intermedia spider venom by immunization with a fused recombinant proteinSimone C Araujo
Departamento de Farmacologia, Universidade Federal de Minas Gerais, CEP 31 270 901, MG, Belo Horizonte, Brazil
Toxicon 41:261-7. 2003..intermedia venom... - Production of monoclonal antibodies capable of neutralizing dermonecrotic activity of Loxosceles intermedia spider venom and their use in a specific immunometric assayLarissa M Alvarenga
, ICB, Universidade Federal de Minas Gerais, CEP 31270901, Belo Horizonte-MG, Brazil
Toxicon 42:725-31. 2003..intermedia IgGs prepared by immunoaffinity chromatography and conjugated to horseradish peroxidase were used to set up a sandwich-type ELISA. Measurable absorbance signals were obtained with 0.2 ng of L. intermedia crude venom per assay... 
Vitamin D3 and androgen receptors in testis and epididymal region of roosters (Gallus domesticus) as affected by epididymal lithiasisAndré G Oliveira
Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
Anim Reprod Sci 109:343-55. 2008..The AR were increased, however, mainly in the epididymal duct epithelium. These results suggest that the vitamin D3 and androgen responsive system may be directly/indirectly involved in the development of the disease...- Identification and molecular cloning of insecticidal toxins from the venom of the brown spider Loxosceles intermediaCibele Soares de Castro
Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, , Belo Horizonte Cep: 31270901, MG, Brazil
Toxicon 44:273-80. 2004..The amino acid sequence deduced from the cDNA of LiTx x 1, LiTx x 2 and LiTx x 3 revealed mature proteins of approximately 7.4, 7.9 and 5.6 kDa... - Determination of sphingomyelinase-D activity of Loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidaseMarco Túlio R Gomes
Departamento de Bioquímica Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270 901, Minas Gerais, Brazil
Toxicon 57:574-9. 2011..However, Tityus serrulatus scorpion venom, Phoneutria nigriventer spider venom and Bothrops jararaca, Crotalus durissus, Lachesis muta and Micrurus frontalis snake venoms did not show measurable SMase-D activity... - An in vivo protective response against toxic effects of the dermonecrotic protein from Loxosceles intermedia spider venom elicited by synthetic epitopesLiza Felicori
Departamento de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, Brazil
Vaccine 27:4201-8. 2009..These results encourage us in the utilization of synthetic peptides for therapeutic serum development or vaccination approaches... 
Effects of 3-beta-diol, an androgen metabolite with intrinsic estrogen-like effects, in modulating the aquaporin-9 expression in the rat efferent ductulesPatrícia Picciarelli-Lima
Department of Morphology, Federal University of Minas Gerais, CX, Postal 486, CEP 31, 270 901, Belo Horizonte, Minas Gerais, Brazil
Reprod Biol Endocrinol 4:51. 2006..Considering that both ER-alpha and ER-beta are highly expressed in efferent ductules, we hypothesized that the DHT regulation of AQP9 could be due to the 3-beta-diol metabolite...- Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1Camila Dias-Lopes
Biochemistry Departament, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, CP 486, CEP 30161 970, Belo Horizonte, Brazil
Toxicon 56:1426-35. 2010..Thus, L. intermedia spider venom and the Loxtox rLiD1 play a key role in heart dysfunction... - Phoneutria nigriventer venom: a cocktail of toxins that affect ion channelsMarcus V Gomez
Departamento de Farmacologia, Instituto de Ciências Biológicas Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
Cell Mol Neurobiol 22:579-88. 2002..4. It is expected that molecular genetics in addition to biochemical, biophysical and pharmacological approaches will help to further define Phoneutria toxins and their mechanisms of action in the near future... - Molecular cloning and characterization of Phoneutria nigriventer toxins active on calcium channelsF C Cardoso
Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Belo Horizonte, Minas Gerais 31 270 900, Brazil
Toxicon 41:755-63. 2003..In addition, the toxin Pn3-5A showed 58% identity to the Tx3-5 amino acid sequence, and the toxins Pn3-6A and Pn3-6B showed 85 and 33% identity, respectively, to the Tx3-6 amino acid sequence... - Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversityFlávia F Siqueira
Universidade Federal de Minas Gerais, UFMG, Instituto de Ciencias Biologicas, Departamento de Biologia Geral, Laboratório de Biotecnologia e Marcadores Moleculares, Av Antonio Carlos 6627, Campus da UFMG, CEP 30123 970, Belo Horizonte, Minas Gerais, Brazil
Vet Microbiol 159:397-405. 2012..perfringens plc gene resulting in the identification of various isoforms. A better understanding of sequences encoding phospholipase C isoforms may reveal changes associated with protein function and C. perfringens virulence... - Distribution of vitamin D3 receptor in the epididymal region of roosters (Gallus domesticus) is cell and segment specificRubem A P Dornas
Department of Morphology, Federal University of Minas Gerais, Av Antonio Carlos 6627, Cx Postal 486, CEP 31270 901, Belo Horizonte, MG Brazil
Gen Comp Endocrinol 150:414-8. 2007.... - Effects of a Lasiodora spider venom on Ca2+ and Na+ channelsC Kushmerick
Departamento de Farmacologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Toxicon 39:991-1002. 2001..The observed effects of this venom on whole-cell currents explain the changes it causes in intracellular Ca2+ in GH3 cells and demonstrate that the venom of this spider is a source of toxins active against ion channels... - Molecular cloning and expression of epsilon toxin from Clostridium perfringens type D and tests of animal immunizationA M Souza
Laboratório de Marcadores Moleculares e Biotecnologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
Genet Mol Res 9:266-76. 2010..This rec-epsilon is a good candidate for vaccine production against enterotoxemia caused by epsilon toxin of C. perfringens type D... - Characterization of the venom from the Brazilian Brown Spider Loxosceles similis Moenkhaus, 1898 (Araneae, Sicariidae)F G Silvestre
Departamento de Biologia Geral, , ICB, Universidade Federal de Minas Gerais, , 6627, Belo Horizonte, MG, CEP 31270901, Brazil
Toxicon 46:927-36. 2005..2 kDa with an estimated pI of 7.37 and sequence similar to LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom, a synanthropic species of medical importance... - Purification, amino-acid sequence and partial characterization of two toxins with anti-insect activity from the venom of the South American scorpion Tityus bahiensis (Buthidae)A M Pimenta
Departamento de Fisiologia e Biofisica, ICB, Universidade Federal de Minas Gerais, 31270 901, MG, Belo Horizonte, Brazil
Toxicon 39:1009-19. 2001..Based on the available comparative information, this study attempts identify possible structure-function relationships that may be responsible for the differences in bioactivity displayed by these toxins... - Differential effects of Tityus bahiensis scorpion venom on tetrodotoxin-sensitive and tetrodotoxin-resistant sodium currentsEder R Moraes
Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 31270 901 Belo Horizonte, MG, Brazil
Neurotox Res 19:102-14. 2011..The cardiac tetrodotoxin-resistant current has complex sensitivity that includes both inhibition of current amplitude and slowing of activation and inactivation... - Effects of the venom of a Mygalomorph spider (Lasiodora sp.) on the isolated rat heartEvanguedes Kalapothakis
Departamento de Farmacologia, , Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Pampulha, 31270-901, MG, Belo Horizonte, Brazil
Toxicon 41:23-8. 2003..Together, these data suggest that this Lasiodora venom evokes vesicular release of acetylcholine from parasympathetic nerve terminals by activating tetrodotoxin-resistant Na(+) channels... - A new and fast technique to generate offspring after germ cells transplantation in adult fish: the Nile tilapia (Oreochromis niloticus) modelSamyra M S N Lacerda
Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
PLoS ONE 5:e10740. 2010..niloticus), in which endogenous spermatogenesis had been depleted with the cytostatic drug busulfan... - Potency against enterotoxemia of a recombinant Clostridium perfringens type D epsilon toxoid in ruminantsFrancisco C F Lobato
Laboratory of Bacteriology, Department of Preventive Veterinary Medicine, Veterinary School of Federal University of Minas Gerais, Antônio Carlos Avenue, 6627, Belo Horizonte 30123 970, MG, Brazil
Vaccine 28:6125-7. 2010..Antibody titers were 40, 14.3, 26, and 13.1 IU/mL in the rabbit, goat, sheep, and cattle serum pools, respectively. The epsilon toxoid produced and tested in this work is adequate for immunization of ruminants against enterotoxemia... - Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2-6Alessandra Matavel
Department of Biochemistry and Immunology, , Universidade Federal de Minas Gerais, Caixa Postal 486, 30161-970, MG, Belo Horizonte, Brazil
FEBS Lett 523:219-23. 2002..The concentration dependence of the modified current suggests a K(D) of 0.8 microM for the toxin-channel complex... - Epitope mapping of the antigenic protein TsNTxP from Tityus serrulatus scorpion venom using mouse, rabbit and sheep antibodiesT M Mendes
, ICB, Universidade Federal de Minas Gerais, , 31270-901 Belo Horizonte, MG, Brazil
Toxicon 44:617-24. 2004..In addition, in vitro neutralization assays indicated that sheep are an alternative and efficient model for the production of anti-Tityus serrulatus venom... - Expression of a functional recombinant Phoneutria nigriventer toxin active on K+ channelsA M D Carneiro
, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Belo Horizonte, 31270-901, Minas Gerais, Brazil
Toxicon 41:305-13. 2003..The recombinant ISEF-PnTx3-1 should be useful for further understanding of the role of A-type K(+) currents in biological processes... - Functional characterization and epitope analysis of a recombinant dermonecrotic protein from Loxosceles intermedia spiderL Felicori
, Universidade Federal de Minas Gerais, CEP 30161-970, Belo Horizonte, MG, Brazil
Toxicon 48:509-19. 2006..The amino acid sequence of this epitope was found in several dermonecrotic proteins and some of its residues have been implicated with the active site of the toxin... - Spider neurotoxins block the beta scorpion toxin-induced calcium uptake in rat brain cortical synaptosomesD M Miranda
, Departamento de Farmacologia, ICB-UFMG, MG, Belo Horizonte, Brazil
Brain Res Bull 54:533-6. 2001..0 and 30.0 nM, respectively. It is suggested that spider neurotoxins Tx3-3 and Tx3-4 blocking effect in the TiTX gamma-induced calcium uptake involves P/Q-type calcium channels... - A protective immune response against lethal, dermonecrotic and hemorrhagic effects of Loxosceles intermedia venom elicited by a 27-residue peptideC Dias-Lopes
Departamento de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, CP 486 CEP 31270901 Belo Horizonte MG, Brazil
Toxicon 55:481-7. 2010..The possible mechanisms of neutralization are discussed in light of these findings... - Preclinical testing of Peruvian anti-bothropic anti-venom against Bothrops andianus snake venomFrancisco S Schneider
Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, CP 486, CEP 31270 901, Belo Horizonte, Minas Gerais, Brazil
Toxicon 60:1018-21. 2012..The Peruvian anti-bothropic anti-venom displays consistent cross-reactivity with B. andianus venom, by ELISA and Western Blotting and is also effective in neutralizing the venom's toxic activities... - Molecular cloning of a mitogenic proteinase from Carica candamarcensis: its potential use in wound healingNatássia C R Corrêa
Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, UFMG 31270 901, Belo Horizonte, MG, Brazil
Phytochemistry 72:1947-54. 2011..The mitogenic activity of this protein and its lack of proteolytic activity underscore a potential for use in wound healing treatment... - Expression of different classes of immunoglobulin in intraepithelial plasma cells of the Harderian gland of domestic ducks Anas platyrhynchosCleida A Oliveira
Departamento de Morfologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, CEP 31270 901 Belo Horizonte, MG, Brazil
Vet Immunol Immunopathol 113:257-66. 2006..The constancy of intraepithelial plasma cells in all specimens examined indicates that they may be essential mediator for an effective immunesurvaillance of the ocular mucosa... - Identification of immunogenic proteins from Paracoccidioides brasiliensis antigenic fractions F0, FII and FIIIT S Goes
, , Universidade Federal de Minas Gerais, Caixa Postal 486, CEP 31 270-901 Belo Horizonte, MG, Brazil
Immunol Lett 101:24-31. 2005..Prediction of transmembrane regions with the prediction transmembrane protein topology with a hidden markov model (TMHMM) revealed consensus sequences representing structural membrane segments in 28 encoded proteins... - Effective Tityus serrulatus anti-venom produced using the Ts1 componentT M Mendes
Departamento de Biologia Geral Genética, ICB, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627 Pampulha, 31270 901 Belo Horizonte, MG, Brazil
Toxicon 52:787-93. 2008..serrulatus crude venom and native Ts1. Thus, Ts1 could be used in a mixed "cocktail" of immunogens for T. serrulatus anti-venom production... - Sandwich-ELISA detection of venom antigens in envenoming by Phoneutria nigriventer spiderC Chavez-Olortegui
Fundação Ezequiel Dias FUNED, Rua Conde Pereira Carneiro 80, Gameleira, 30510 010, Belo Horizonte, MG, Brazil
Toxicon 39:909-11. 2001..8ng of venom per assay. The ELISA was used to follow the kinetic distribution of antigens in experimentally envenomed mice and to detect antigens in the sera of patients envenomed by P. nigriventer... - Molecular characterization of a neutralizing murine monoclonal antibody against Tityus serrulatus scorpion venomL M Alvarenga
, ICB, Universidade Federal de Minas Gerais, CP: 486; CEP: 31270901, Belo Horizonte-MG, Brazil
Toxicon 46:664-71. 2005..The neutralizing properties of mAbTs1 might be explained by spatial vicinity of epitope residues with pharmacophore residues... - In vitro effect of the Phoneutria nigriventer spider venom on cell viability, paracellular barrier function and transcellular transport in cultured cell linesLuciana de Paula Le Sueur
Departamento de Histologia e Embriologia, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP, CEP 13083-970 Campinas, SP, Brazil
Toxicon 46:130-41. 2005..We conclude that the venom of P. nigriventer displays a relatively low cytotoxicity in vitro as well as activates directly the endocytic transport pathway in MDCK cells without disrupting the paracellular route... - Breakdown of the blood-brain barrier and neuropathological changes induced by Phoneutria nigriventer spider venomLuciana de Paula Le Sueur
Departamento de Histologia e Embriologia, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP, S.P, CEP 13083-970, Brasil
Acta Neuropathol (Berl) 105:125-34. 2003..This study demonstrates that systemic PNV inoculation induces BBB breakdown through trans- and paracellular routes. It is concluded that BBB breakdown is an event not associated with the acute neurotoxicity exhibited by the rats...