Evanguedes Kalapothakis

Summary

Affiliation: Belo Horizonte
Country: Brazil

Publications

  1. ncbi request reprint Screening of expression libraries using ELISA: identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom
    E Kalapothakis
    Divisao de Biologia Celular, Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, MG 31 270901, Belo Horizonte, Brazil
    Toxicon 39:679-85. 2001
  2. ncbi request reprint Brown spider dermonecrotic toxin directly induces nephrotoxicity
    Olga Meiri Chaim
    Department of Cell Biology, Federal University of Parana, Jardim das Americas, 81531 990, Curitiba, Parana, Brazil
    Toxicol Appl Pharmacol 211:64-77. 2006
  3. ncbi request reprint Evaluation of Brazilian biotechnology patent activity from 1975 to 2010
    F Dias
    Departamento de Biologia Geral, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    Recent Pat DNA Gene Seq 6:145-59. 2012
  4. ncbi request reprint The Loxtox protein family in Loxosceles intermedia (Mello-Leitão) venom
    E Kalapothakis
    Departamento de Biologia Geral Instituto de Ciências Biológicas Universidade Federal de Minas Gerais, CEP 31270901 Belo Horizonte, MG, Brazil
    Toxicon 50:938-46. 2007
  5. ncbi request reprint Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom
    Evanguedes Kalapothakis
    Departamento de Farmacologia, Divisao de Biologia Celular, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Belo Horizonte, MG, CEP 31270901, Brazil
    Toxicon 40:1691-9. 2002
  6. ncbi request reprint Identification and molecular cloning of insecticidal toxins from the venom of the brown spider Loxosceles intermedia
    Cibele Soares de Castro
    Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Belo Horizonte Cep 31270901, MG, Brazil
    Toxicon 44:273-80. 2004
  7. doi request reprint Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversity
    Flávia F Siqueira
    Universidade Federal de Minas Gerais, UFMG, Instituto de Ciencias Biologicas, Departamento de Biologia Geral, Laboratório de Biotecnologia e Marcadores Moleculares, Av Antonio Carlos 6627, Campus da UFMG, CEP 30123 970, Belo Horizonte, Minas Gerais, Brazil
    Vet Microbiol 159:397-405. 2012
  8. ncbi request reprint Production of monoclonal antibodies capable of neutralizing dermonecrotic activity of Loxosceles intermedia spider venom and their use in a specific immunometric assay
    Larissa M Alvarenga
    Departamentos de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, CEP 31270901, Belo Horizonte MG, Brazil
    Toxicon 42:725-31. 2003
  9. doi request reprint Vitamin D3 and androgen receptors in testis and epididymal region of roosters (Gallus domesticus) as affected by epididymal lithiasis
    André G Oliveira
    Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
    Anim Reprod Sci 109:343-55. 2008
  10. ncbi request reprint Protection against dermonecrotic and lethal activities of Loxosceles intermedia spider venom by immunization with a fused recombinant protein
    Simone C Araujo
    Departamento de Farmacologia, Universidade Federal de Minas Gerais, CEP 31 270 901, MG, Belo Horizonte, Brazil
    Toxicon 41:261-7. 2003

Collaborators

  • Cleida A Oliveira
  • Rex A Hess
  • Claude Granier
  • Francisco C F Lobato
  • Marco Túlio R Gomes
  • Marcus V Gomez
  • Jader S Cruz
  • M A Romano-Silva
  • Carlos E Salas
  • K C Barbaro
  • Frederic Frezard
  • Tânia M Segatelli
  • Cristina Guatimosim
  • Luiz R Franca
  • S S Veiga
  • A M Pimenta
  • F Mesquita de Carvalho
  • C Chavez-Olortegui
  • Carlos Chavez-Olortegui
  • André G Oliveira
  • T M Mendes
  • F Dias
  • Liza Felicori
  • Luciana de Paula Le Sueur
  • L Felicori
  • Rubem A P Dornas
  • Germán A B Mahecha
  • Flávia F Siqueira
  • Francisco S Schneider
  • C C Horta
  • Natássia C R Corrêa
  • Eder R Moraes
  • Clara G Duarte
  • L M Alvarenga
  • Samyra M S N Lacerda
  • C Dias-Lopes
  • A M Souza
  • Camila Dias-Lopes
  • Oldemir C Mangili
  • C R Diniz
  • C Kushmerick
  • R A Assis
  • Patrícia Picciarelli-Lima
  • Olga Meiri Chaim
  • R A Machado de Avila
  • F G Silvestre
  • T S Goes
  • Cibele Soares de Castro
  • Maria Alice da Cruz-Höfling
  • Simone C Araujo
  • M De Maria
  • F C Cardoso
  • A M D Carneiro
  • Larissa M Alvarenga
  • W S Maria
  • M N Cordeiro
  • Alessandra Matavel
  • I Drummond
  • Walter Silva Suarez
  • Cesar Bonilla
  • M A Prado
  • Maria C Starling
  • Marcelle O Almeida
  • T M Barroca
  • Silvia Seraylan Ormachea
  • A O Carmo
  • Anderson O Carmo
  • Armando Yarleque
  • Rodrigo O S Silva
  • Carolina C R Horta
  • Benigno Tintaya
  • D M Miranda
  • Tatiana M Barroca
  • Ricardo Machado de Avila
  • F Delfim
  • Prhiscylla S Pires
  • Karin Flores Garrido
  • Christopher Kushmerick
  • Brisa C A Chagas
  • Ligia A Naves
  • Isabela C Mendes
  • Miriam T P Lopes
  • C Contigli
  • E R Alvarenga
  • L Emery
  • Alvair P Almeida
  • F Molina
  • Danilo Roman-Campos
  • Denis Damasceno
  • Guilherme M J Costa

Detail Information

Publications40

  1. ncbi request reprint Screening of expression libraries using ELISA: identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom
    E Kalapothakis
    Divisao de Biologia Celular, Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, MG 31 270901, Belo Horizonte, Brazil
    Toxicon 39:679-85. 2001
    ..The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects...
  2. ncbi request reprint Brown spider dermonecrotic toxin directly induces nephrotoxicity
    Olga Meiri Chaim
    Department of Cell Biology, Federal University of Parana, Jardim das Americas, 81531 990, Curitiba, Parana, Brazil
    Toxicol Appl Pharmacol 211:64-77. 2006
    ....
  3. ncbi request reprint Evaluation of Brazilian biotechnology patent activity from 1975 to 2010
    F Dias
    Departamento de Biologia Geral, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    Recent Pat DNA Gene Seq 6:145-59. 2012
    ..The first biotechnology patent applications via PCT were submitted by Brazilians in 1997, with 3 from UFMG (university), 2 from individuals, and 1 from EMBRAPA (research institute)...
  4. ncbi request reprint The Loxtox protein family in Loxosceles intermedia (Mello-Leitão) venom
    E Kalapothakis
    Departamento de Biologia Geral Instituto de Ciências Biológicas Universidade Federal de Minas Gerais, CEP 31270901 Belo Horizonte, MG, Brazil
    Toxicon 50:938-46. 2007
    ..The finding of several isoforms of Loxtox in the venom of this spider may reflect an evolutionary adaptation for different prey types and reinforces the idea of an efficient mutational mechanism in the venom gland of spiders...
  5. ncbi request reprint Molecular cloning, expression and immunological properties of LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom
    Evanguedes Kalapothakis
    Departamento de Farmacologia, Divisao de Biologia Celular, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Belo Horizonte, MG, CEP 31270901, Brazil
    Toxicon 40:1691-9. 2002
    ..recLiD1 was strongly recognised by anti-dermonecrotic antibodies and was also able to generate reactive antibodies against native dermonecrotic proteins isolated from the venom of L. intermedia...
  6. ncbi request reprint Identification and molecular cloning of insecticidal toxins from the venom of the brown spider Loxosceles intermedia
    Cibele Soares de Castro
    Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Belo Horizonte Cep 31270901, MG, Brazil
    Toxicon 44:273-80. 2004
    ..The amino acid sequence deduced from the cDNA of LiTx x 1, LiTx x 2 and LiTx x 3 revealed mature proteins of approximately 7.4, 7.9 and 5.6 kDa...
  7. doi request reprint Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversity
    Flávia F Siqueira
    Universidade Federal de Minas Gerais, UFMG, Instituto de Ciencias Biologicas, Departamento de Biologia Geral, Laboratório de Biotecnologia e Marcadores Moleculares, Av Antonio Carlos 6627, Campus da UFMG, CEP 30123 970, Belo Horizonte, Minas Gerais, Brazil
    Vet Microbiol 159:397-405. 2012
    ..perfringens plc gene resulting in the identification of various isoforms. A better understanding of sequences encoding phospholipase C isoforms may reveal changes associated with protein function and C. perfringens virulence...
  8. ncbi request reprint Production of monoclonal antibodies capable of neutralizing dermonecrotic activity of Loxosceles intermedia spider venom and their use in a specific immunometric assay
    Larissa M Alvarenga
    Departamentos de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, CEP 31270901, Belo Horizonte MG, Brazil
    Toxicon 42:725-31. 2003
    ..intermedia IgGs prepared by immunoaffinity chromatography and conjugated to horseradish peroxidase were used to set up a sandwich-type ELISA. Measurable absorbance signals were obtained with 0.2 ng of L. intermedia crude venom per assay...
  9. doi request reprint Vitamin D3 and androgen receptors in testis and epididymal region of roosters (Gallus domesticus) as affected by epididymal lithiasis
    André G Oliveira
    Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
    Anim Reprod Sci 109:343-55. 2008
    ..The AR were increased, however, mainly in the epididymal duct epithelium. These results suggest that the vitamin D3 and androgen responsive system may be directly/indirectly involved in the development of the disease...
  10. ncbi request reprint Protection against dermonecrotic and lethal activities of Loxosceles intermedia spider venom by immunization with a fused recombinant protein
    Simone C Araujo
    Departamento de Farmacologia, Universidade Federal de Minas Gerais, CEP 31 270 901, MG, Belo Horizonte, Brazil
    Toxicon 41:261-7. 2003
    ..intermedia venom...
  11. doi request reprint Determination of sphingomyelinase-D activity of Loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidase
    Marco Túlio R Gomes
    Departamento de Bioquímica Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270 901, Minas Gerais, Brazil
    Toxicon 57:574-9. 2011
    ..However, Tityus serrulatus scorpion venom, Phoneutria nigriventer spider venom and Bothrops jararaca, Crotalus durissus, Lachesis muta and Micrurus frontalis snake venoms did not show measurable SMase-D activity...
  12. doi request reprint An in vivo protective response against toxic effects of the dermonecrotic protein from Loxosceles intermedia spider venom elicited by synthetic epitopes
    Liza Felicori
    Departamento de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, Brazil
    Vaccine 27:4201-8. 2009
    ..These results encourage us in the utilization of synthetic peptides for therapeutic serum development or vaccination approaches...
  13. pmc Effects of 3-beta-diol, an androgen metabolite with intrinsic estrogen-like effects, in modulating the aquaporin-9 expression in the rat efferent ductules
    Patrícia Picciarelli-Lima
    Department of Morphology, Federal University of Minas Gerais, CX, Postal 486, CEP 31, 270 901, Belo Horizonte, Minas Gerais, Brazil
    Reprod Biol Endocrinol 4:51. 2006
    ..Considering that both ER-alpha and ER-beta are highly expressed in efferent ductules, we hypothesized that the DHT regulation of AQP9 could be due to the 3-beta-diol metabolite...
  14. doi request reprint Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1
    Camila Dias-Lopes
    Biochemistry Departament, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, CP 486, CEP 30161 970, Belo Horizonte, Brazil
    Toxicon 56:1426-35. 2010
    ..Thus, L. intermedia spider venom and the Loxtox rLiD1 play a key role in heart dysfunction...
  15. ncbi request reprint Phoneutria nigriventer venom: a cocktail of toxins that affect ion channels
    Marcus V Gomez
    Departamento de Farmacologia, Instituto de Ciências Biológicas Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
    Cell Mol Neurobiol 22:579-88. 2002
    ..4. It is expected that molecular genetics in addition to biochemical, biophysical and pharmacological approaches will help to further define Phoneutria toxins and their mechanisms of action in the near future...
  16. ncbi request reprint Molecular cloning and characterization of Phoneutria nigriventer toxins active on calcium channels
    F C Cardoso
    Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Belo Horizonte, Minas Gerais 31 270 900, Brazil
    Toxicon 41:755-63. 2003
    ..In addition, the toxin Pn3-5A showed 58% identity to the Tx3-5 amino acid sequence, and the toxins Pn3-6A and Pn3-6B showed 85 and 33% identity, respectively, to the Tx3-6 amino acid sequence...
  17. ncbi request reprint Distribution of vitamin D3 receptor in the epididymal region of roosters (Gallus domesticus) is cell and segment specific
    Rubem A P Dornas
    Department of Morphology, Federal University of Minas Gerais, Av Antonio Carlos 6627, Cx Postal 486, CEP 31270 901, Belo Horizonte, MG Brazil
    Gen Comp Endocrinol 150:414-8. 2007
    ....
  18. ncbi request reprint Effects of a Lasiodora spider venom on Ca2+ and Na+ channels
    C Kushmerick
    Departamento de Farmacologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    Toxicon 39:991-1002. 2001
    ..The observed effects of this venom on whole-cell currents explain the changes it causes in intracellular Ca2+ in GH3 cells and demonstrate that the venom of this spider is a source of toxins active against ion channels...
  19. doi request reprint Preclinical testing of Peruvian anti-bothropic anti-venom against Bothrops andianus snake venom
    Francisco S Schneider
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, CP 486, CEP 31270 901, Belo Horizonte, Minas Gerais, Brazil
    Toxicon 60:1018-21. 2012
    ..The Peruvian anti-bothropic anti-venom displays consistent cross-reactivity with B. andianus venom, by ELISA and Western Blotting and is also effective in neutralizing the venom's toxic activities...
  20. ncbi request reprint Characterization of the venom from the Brazilian Brown Spider Loxosceles similis Moenkhaus, 1898 (Araneae, Sicariidae)
    F G Silvestre
    Departamento de Biologia Geral, Instituto de Ciencias Biologicas, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Belo Horizonte, MG, CEP 31270901, Brazil
    Toxicon 46:927-36. 2005
    ..2 kDa with an estimated pI of 7.37 and sequence similar to LiD1, a protein from the dermonecrotic family of Loxosceles intermedia spider venom, a synanthropic species of medical importance...
  21. ncbi request reprint Purification, amino-acid sequence and partial characterization of two toxins with anti-insect activity from the venom of the South American scorpion Tityus bahiensis (Buthidae)
    A M Pimenta
    Departamento de Fisiologia e Biofisica, ICB, Universidade Federal de Minas Gerais, 31270 901, MG, Belo Horizonte, Brazil
    Toxicon 39:1009-19. 2001
    ..Based on the available comparative information, this study attempts identify possible structure-function relationships that may be responsible for the differences in bioactivity displayed by these toxins...
  22. doi request reprint Molecular cloning and expression of epsilon toxin from Clostridium perfringens type D and tests of animal immunization
    A M Souza
    Laboratório de Marcadores Moleculares e Biotecnologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
    Genet Mol Res 9:266-76. 2010
    ..This rec-epsilon is a good candidate for vaccine production against enterotoxemia caused by epsilon toxin of C. perfringens type D...
  23. doi request reprint Differential effects of Tityus bahiensis scorpion venom on tetrodotoxin-sensitive and tetrodotoxin-resistant sodium currents
    Eder R Moraes
    Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 31270 901 Belo Horizonte, MG, Brazil
    Neurotox Res 19:102-14. 2011
    ..The cardiac tetrodotoxin-resistant current has complex sensitivity that includes both inhibition of current amplitude and slowing of activation and inactivation...
  24. pmc A new and fast technique to generate offspring after germ cells transplantation in adult fish: the Nile tilapia (Oreochromis niloticus) model
    Samyra M S N Lacerda
    Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    PLoS ONE 5:e10740. 2010
    ..niloticus), in which endogenous spermatogenesis had been depleted with the cytostatic drug busulfan...
  25. doi request reprint Potency against enterotoxemia of a recombinant Clostridium perfringens type D epsilon toxoid in ruminants
    Francisco C F Lobato
    Laboratory of Bacteriology, Department of Preventive Veterinary Medicine, Veterinary School of Federal University of Minas Gerais, Antônio Carlos Avenue, 6627, Belo Horizonte 30123 970, MG, Brazil
    Vaccine 28:6125-7. 2010
    ..Antibody titers were 40, 14.3, 26, and 13.1 IU/mL in the rabbit, goat, sheep, and cattle serum pools, respectively. The epsilon toxoid produced and tested in this work is adequate for immunization of ruminants against enterotoxemia...
  26. ncbi request reprint Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2-6
    Alessandra Matavel
    Department of Biochemistry and Immunology, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Caixa Postal 486, 30161 970, MG, Belo Horizonte, Brazil
    FEBS Lett 523:219-23. 2002
    ..The concentration dependence of the modified current suggests a K(D) of 0.8 microM for the toxin-channel complex...
  27. ncbi request reprint Effects of the venom of a Mygalomorph spider (Lasiodora sp.) on the isolated rat heart
    Evanguedes Kalapothakis
    Departamento de Farmacologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Pampulha, 31270 901, MG, Belo Horizonte, Brazil
    Toxicon 41:23-8. 2003
    ..Together, these data suggest that this Lasiodora venom evokes vesicular release of acetylcholine from parasympathetic nerve terminals by activating tetrodotoxin-resistant Na(+) channels...
  28. ncbi request reprint Spider neurotoxins block the beta scorpion toxin-induced calcium uptake in rat brain cortical synaptosomes
    D M Miranda
    , Departamento de Farmacologia, ICB-UFMG, MG, Belo Horizonte, Brazil
    Brain Res Bull 54:533-6. 2001
    ..0 and 30.0 nM, respectively. It is suggested that spider neurotoxins Tx3-3 and Tx3-4 blocking effect in the TiTX gamma-induced calcium uptake involves P/Q-type calcium channels...
  29. doi request reprint A protective immune response against lethal, dermonecrotic and hemorrhagic effects of Loxosceles intermedia venom elicited by a 27-residue peptide
    C Dias-Lopes
    Departamento de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, CP 486 CEP 31270901 Belo Horizonte MG, Brazil
    Toxicon 55:481-7. 2010
    ..The possible mechanisms of neutralization are discussed in light of these findings...
  30. ncbi request reprint Expression of a functional recombinant Phoneutria nigriventer toxin active on K+ channels
    A M D Carneiro
    Departamento de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Belo Horizonte, 31270 901, Minas Gerais, Brazil
    Toxicon 41:305-13. 2003
    ..The recombinant ISEF-PnTx3-1 should be useful for further understanding of the role of A-type K(+) currents in biological processes...
  31. ncbi request reprint Epitope mapping of the antigenic protein TsNTxP from Tityus serrulatus scorpion venom using mouse, rabbit and sheep antibodies
    T M Mendes
    Departamento de Biologia Geral Genética, ICB, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627 Pampulha, 31270 901 Belo Horizonte, MG, Brazil
    Toxicon 44:617-24. 2004
    ..In addition, in vitro neutralization assays indicated that sheep are an alternative and efficient model for the production of anti-Tityus serrulatus venom...
  32. ncbi request reprint Functional characterization and epitope analysis of a recombinant dermonecrotic protein from Loxosceles intermedia spider
    L Felicori
    Departamentos de Bioquímica Imunologia, Universidade Federal de Minas Gerais, CEP 30161 970, Belo Horizonte, MG, Brazil
    Toxicon 48:509-19. 2006
    ..The amino acid sequence of this epitope was found in several dermonecrotic proteins and some of its residues have been implicated with the active site of the toxin...
  33. ncbi request reprint Expression of different classes of immunoglobulin in intraepithelial plasma cells of the Harderian gland of domestic ducks Anas platyrhynchos
    Cleida A Oliveira
    Departamento de Morfologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, CEP 31270 901 Belo Horizonte, MG, Brazil
    Vet Immunol Immunopathol 113:257-66. 2006
    ..The constancy of intraepithelial plasma cells in all specimens examined indicates that they may be essential mediator for an effective immunesurvaillance of the ocular mucosa...
  34. doi request reprint Molecular cloning of a mitogenic proteinase from Carica candamarcensis: its potential use in wound healing
    Natássia C R Corrêa
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, UFMG 31270 901, Belo Horizonte, MG, Brazil
    Phytochemistry 72:1947-54. 2011
    ..The mitogenic activity of this protein and its lack of proteolytic activity underscore a potential for use in wound healing treatment...
  35. ncbi request reprint Sandwich-ELISA detection of venom antigens in envenoming by Phoneutria nigriventer spider
    C Chavez-Olortegui
    Fundação Ezequiel Dias FUNED, Rua Conde Pereira Carneiro 80, Gameleira, 30510 010, Belo Horizonte, MG, Brazil
    Toxicon 39:909-11. 2001
    ..8ng of venom per assay. The ELISA was used to follow the kinetic distribution of antigens in experimentally envenomed mice and to detect antigens in the sera of patients envenomed by P. nigriventer...
  36. ncbi request reprint Molecular characterization of a neutralizing murine monoclonal antibody against Tityus serrulatus scorpion venom
    L M Alvarenga
    Departamentos de Bioquímica Imunologia, ICB, Universidade Federal de Minas Gerais, CP 486 CEP 31270901, Belo Horizonte MG, Brazil
    Toxicon 46:664-71. 2005
    ..The neutralizing properties of mAbTs1 might be explained by spatial vicinity of epitope residues with pharmacophore residues...
  37. doi request reprint Effective Tityus serrulatus anti-venom produced using the Ts1 component
    T M Mendes
    Departamento de Biologia Geral Genética, ICB, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627 Pampulha, 31270 901 Belo Horizonte, MG, Brazil
    Toxicon 52:787-93. 2008
    ..serrulatus crude venom and native Ts1. Thus, Ts1 could be used in a mixed "cocktail" of immunogens for T. serrulatus anti-venom production...
  38. ncbi request reprint Identification of immunogenic proteins from Paracoccidioides brasiliensis antigenic fractions F0, FII and FIII
    T S Goes
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Caixa Postal 486, Cep 31 270 901 Belo Horizonte, MG, Brazil
    Immunol Lett 101:24-31. 2005
    ..Prediction of transmembrane regions with the prediction transmembrane protein topology with a hidden markov model (TMHMM) revealed consensus sequences representing structural membrane segments in 28 encoded proteins...
  39. ncbi request reprint In vitro effect of the Phoneutria nigriventer spider venom on cell viability, paracellular barrier function and transcellular transport in cultured cell lines
    Luciana de Paula Le Sueur
    Departamento de Histologia e Embriologia, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas UNICAMP, CEP 13083 970 Campinas, SP, Brazil
    Toxicon 46:130-41. 2005
    ..We conclude that the venom of P. nigriventer displays a relatively low cytotoxicity in vitro as well as activates directly the endocytic transport pathway in MDCK cells without disrupting the paracellular route...
  40. ncbi request reprint Breakdown of the blood-brain barrier and neuropathological changes induced by Phoneutria nigriventer spider venom
    Luciana de Paula Le Sueur
    Departamento de Histologia e Embriologia, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas UNICAMP, S P, CEP 13083 970, Brasil
    Acta Neuropathol 105:125-34. 2003
    ..This study demonstrates that systemic PNV inoculation induces BBB breakdown through trans- and paracellular routes. It is concluded that BBB breakdown is an event not associated with the acute neurotoxicity exhibited by the rats...