Laurent Blanchoin

Summary

Publications

  1. ncbi request reprint Phosphorylation of microtubule-associated protein STOP by calmodulin kinase II
    Julie Baratier
    Laboratoire du Cytosquelette, INSERM U366, DRDC CS, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    J Biol Chem 281:19561-9. 2006
  2. ncbi request reprint Actin dynamics: old friends with new stories
    Christopher J Staiger
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Curr Opin Plant Biol 9:554-62. 2006
  3. pmc Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover
    Parul Khurana
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Plant Cell 22:2727-48. 2010
  4. pmc Arabidopsis actin depolymerizing factor4 modulates the stochastic dynamic behavior of actin filaments in the cortical array of epidermal cells
    Jessica L Henty
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Plant Cell 23:3711-26. 2011
  5. pmc Identification of Arabidopsis cyclase-associated protein 1 as the first nucleotide exchange factor for plant actin
    Faisal Chaudhry
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2064, USA
    Mol Biol Cell 18:3002-14. 2007
  6. pmc Arabidopsis VILLIN1 generates actin filament cables that are resistant to depolymerization
    Shanjin Huang
    Department of Biological Sciences and Purdue Motility Group, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Plant Cell 17:486-501. 2005
  7. pmc Capping protein modulates the dynamic behavior of actin filaments in response to phosphatidic acid in Arabidopsis
    Jiejie Li
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2064, USA
    Plant Cell 24:3742-54. 2012
  8. pmc Heterodimeric capping protein from Arabidopsis is regulated by phosphatidic acid
    Shanjin Huang
    Department of Biological Sciences and The Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907 2064, USA
    Mol Biol Cell 17:1946-58. 2006
  9. doi request reprint Regulation of actin dynamics by actin-binding proteins in pollen
    Christopher J Staiger
    Department of Biological Sciences and Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907 2064, USA
    J Exp Bot 61:1969-86. 2010
  10. pmc Arabidopsis capping protein senses cellular phosphatidic acid levels and transduces these into changes in actin cytoskeleton dynamics
    Jiejie Li
    Department of Biological Sciences, Purdue University, West Lafayette, IN, USA
    Plant Signal Behav 7:1727-30. 2012

Collaborators

  • M Vantard
  • Christopher J Staiger
  • Einat Sadot
  • Brad Day
  • David W McCurdy
  • Richard B Meagher
  • David Kovar
  • Noni Franklin-Tong
  • Thomas Pollard
  • James Bamburg
  • Shanjin Huang
  • Jiejie Li
  • Jessica L Henty-Ridilla
  • Alphée Michelot
  • Christophe Guerin
  • Jessica L Henty
  • Parul Khurana
  • Faisal Chaudhry
  • Mathieu Ingouff
  • Jeremy Roland
  • Brannon R McCullough
  • Jean Louis Martiel
  • Julie Baratier
  • Robert C Robinson
  • Catherine Wicker-Planquart
  • Edward Irobi
  • Shundai Li
  • Giles O C Cory
  • Kyoko Okada
  • Benjamin H Staiger
  • Mohamad Abu-Abied
  • Roman Pleskot
  • Martin Potocky
  • Xia Wang
  • Samuel W Bledsoe
  • Andrew M Staiger
  • Julien Berro
  • Enrique M De La Cruz
  • Matthias von Witsch
  • Fabrice Dufour
  • Christophe Bosc
  • Leticia Peris
  • Sylvie Gory-Fauré
  • Francois Parcy
  • Didier Job
  • Rajaa Paterski-Boujemaa
  • Paul Salin
  • Annie Andrieux
  • Anne Fourest-Lieuvin
  • Lisa Gao
  • Emmanuel Derivery
  • Jacques Brocard
  • Hélène Robert
  • Danny Geelen
  • Tracie Matsumoto
  • Jonathan N Fitz Gerald
  • Daniel Van Damme
  • Natalia Rodiuc
  • Mikael Blom Sørensen
  • Arnaud Brunet
  • Lisa Y Gao
  • Stephane Richard
  • Frederic Berger
  • Virginie Stoppin-Mellet
  • Helen L Yin
  • Adeleke H Aguda
  • Vernonica E Franklin-Tong
  • Mårten Larsson
  • Leslie D Burtnick
  • Zhenbiao Yang
  • Elizabeth M Lord
  • Rainer Cramer
  • Anne J Ridley
  • Hiroshi Abe
  • Hui Chen

Detail Information

Publications27

  1. ncbi request reprint Phosphorylation of microtubule-associated protein STOP by calmodulin kinase II
    Julie Baratier
    Laboratoire du Cytosquelette, INSERM U366, DRDC CS, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    J Biol Chem 281:19561-9. 2006
    ..Thus, STOP phosphorylation by CaMKII may promote STOP translocation from microtubules to synaptic compartments where it may interact with actin, which could be important for STOP function in synaptic plasticity...
  2. ncbi request reprint Actin dynamics: old friends with new stories
    Christopher J Staiger
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Curr Opin Plant Biol 9:554-62. 2006
    ..These and other stories motivate a careful description of the properties of plant proteins in vitro as a prelude to greater insight into the molecular mechanism(s) underlying the regulation of actin dynamics in vivo...
  3. pmc Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover
    Parul Khurana
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Plant Cell 22:2727-48. 2010
    ..Moreover, VLN3 can sever actin filament bundles in the presence of VLN1 when calcium is elevated to micromolar levels. Collectively, these results demonstrate that two villin isovariants have overlapping and distinct activities...
  4. pmc Arabidopsis actin depolymerizing factor4 modulates the stochastic dynamic behavior of actin filaments in the cortical array of epidermal cells
    Jessica L Henty
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Plant Cell 23:3711-26. 2011
    ..5-fold reduced severing frequency as well as significantly increased actin filament lengths and lifetimes. Thus, we provide evidence that ADF4 contributes to the stochastic dynamic turnover of actin filaments in plant cells...
  5. pmc Identification of Arabidopsis cyclase-associated protein 1 as the first nucleotide exchange factor for plant actin
    Faisal Chaudhry
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2064, USA
    Mol Biol Cell 18:3002-14. 2007
    ..Collectively, these results and our understanding of other actin-binding proteins implicate CAP1 as a central player in regulating the pool of unpolymerized ATP-actin...
  6. pmc Arabidopsis VILLIN1 generates actin filament cables that are resistant to depolymerization
    Shanjin Huang
    Department of Biological Sciences and Purdue Motility Group, Purdue University, West Lafayette, Indiana 47907 2064, USA
    Plant Cell 17:486-501. 2005
    ....
  7. pmc Capping protein modulates the dynamic behavior of actin filaments in response to phosphatidic acid in Arabidopsis
    Jiejie Li
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2064, USA
    Plant Cell 24:3742-54. 2012
    ..Specifically, CP acts as a PA biosensor and key transducer of fluxes in membrane signaling phospholipids into changes in actin cytoskeleton dynamics...
  8. pmc Heterodimeric capping protein from Arabidopsis is regulated by phosphatidic acid
    Shanjin Huang
    Department of Biological Sciences and The Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907 2064, USA
    Mol Biol Cell 17:1946-58. 2006
    ..Such regulation may be important for the response of plant cells to extracellular stimuli as well as for the normal process of pollen tube tip growth...
  9. doi request reprint Regulation of actin dynamics by actin-binding proteins in pollen
    Christopher J Staiger
    Department of Biological Sciences and Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907 2064, USA
    J Exp Bot 61:1969-86. 2010
    ..A new model for the dynamic control of actin filament turnover in plant cells is presented...
  10. pmc Arabidopsis capping protein senses cellular phosphatidic acid levels and transduces these into changes in actin cytoskeleton dynamics
    Jiejie Li
    Department of Biological Sciences, Purdue University, West Lafayette, IN, USA
    Plant Signal Behav 7:1727-30. 2012
    ..This allows rapid actin polymerization and increases in filament abundance following stimulation and could be one key factor in the physiological responses of plant cells to environmental stimuli...
  11. ncbi request reprint A gelsolin-like protein from Papaver rhoeas pollen (PrABP80) stimulates calcium-regulated severing and depolymerization of actin filaments
    Shanjin Huang
    Department of Biological Sciences and The Purdue Motility Group, Purdue University, West Lafayette, Indiana 47907 2064, USA
    J Biol Chem 279:23364-75. 2004
    ..We propose that PrABP80 functions at the center of the self-incompatibility response by creating new filament pointed ends for disassembly and by blocking barbed ends from profilin-actin assembly...
  12. pmc The availability of filament ends modulates actin stochastic dynamics in live plant cells
    Jiejie Li
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2064 Institute of Plant Sciences, Volcani Center, Bet Dagan 50250, Israel Institut de Recherches en Technologie et Sciences pour le Vivant, Laboratoire de Physiologie Cellulaire et Végétale, Comissariat a l Energie Atomique Centre National de la Recherche Scientifique Institute de la Recherche Agronomique Université Joseph Fourier, F38054 Grenoble, France Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907
    Mol Biol Cell 25:1263-75. 2014
    ..On the basis of these and other genetic studies in this model system, we hypothesize that filament length and lifetime positively correlate with the extent of axial cell expansion in dark-grown hypocotyls. ..
  13. doi request reprint Actin dynamics in the cortical array of plant cells
    Jessica L Henty-Ridilla
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2064, USA
    Curr Opin Plant Biol 16:678-87. 2013
    ..Collectively, the latest data emphasize the importance of filament severing activities and regulation of barbed-end availability as key facets of plant actin filament turnover. ..
  14. pmc Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array
    Christopher J Staiger
    Department of Biological Sciences, Hansen Life Sciences Research Building, Purdue University, West Lafayette, IN 47907, USA
    J Cell Biol 184:269-80. 2009
    ..These observations indicate a mechanism inconsistent with treadmilling. Instead, cortical actin filament dynamics resemble the stochastic dynamics of an in vitro biomimetic system for actin assembly...
  15. ncbi request reprint Arabidopsis capping protein (AtCP) is a heterodimer that regulates assembly at the barbed ends of actin filaments
    Shanjin Huang
    Department of Biological Sciences and Purdue Motility Group, Purdue University, West Lafayette, Indiana 47907 2064, USA
    J Biol Chem 278:44832-42. 2003
    ..We propose that AtCP is a major regulator of actin dynamics in plant cells that, together with abundant profilin, is responsible for maintaining a large pool of actin subunits and a surprisingly small population of F-actin...
  16. pmc Cofilin increases the bending flexibility of actin filaments: implications for severing and cell mechanics
    Brannon R McCullough
    Yale University, Department of Molecular Biophysics and Biochemistry, 260 Whitney Avenue, New Haven, CT 06520, USA
    J Mol Biol 381:550-8. 2008
    ....
  17. pmc Stochastic severing of actin filaments by actin depolymerizing factor/cofilin controls the emergence of a steady dynamical regime
    Jeremy Roland
    Universite Joseph Fourier, TIMC IMAG Laboratory, Grenoble, France
    Biophys J 94:2082-94. 2008
    ..Our analysis shows that ADF/cofilin regulation maintains actin filaments in a highly dynamical state compatible with the cytoskeleton dynamics observed in vivo...
  18. ncbi request reprint A novel mechanism for the formation of actin-filament bundles by a nonprocessive formin
    Alphée Michelot
    Département Réponse et Dynamique Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Joseph Fourier, F38054 Grenoble, France
    Curr Biol 16:1924-30. 2006
    ..We show why this new mechanism of nucleation by a member of the formin family is important for bundle formation. Finally, we analyze the different parameters controlling the dynamic formation of such longitudinal actin-filament bundles...
  19. ncbi request reprint Xenopus actin-interacting protein 1 (XAip1) enhances cofilin fragmentation of filaments by capping filament ends
    Kyoko Okada
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 277:43011-6. 2002
    ..We conclude that XAip1 caps the barbed end of filaments severed by cofilin. This capping blocks annealing and depolymerization and allows more extensive severing by cofilin...
  20. ncbi request reprint Actin polymerization processes in plant cells
    Marylin Vantard
    Laboratoire de Physiologie Cellulaire Végétale, UMR 5019, CEA CNRS UJF, DRDC CEA de Grenoble, 17 rue des Martyrs, 38054, Grenoble, France
    Curr Opin Plant Biol 5:502-6. 2002
    ....
  21. ncbi request reprint Phosphorylation of the WASP-VCA domain increases its affinity for the Arp2/3 complex and enhances actin polymerization by WASP
    Giles O C Cory
    Ludwig Institute for Cancer Research, Royal Free and University College Medical School Branch, Courtauld Building, 91 Riding House Street, London W1W 7BS, United Kingdom
    Mol Cell 11:1229-39. 2003
    ..We propose that constitutive VCA domain phosphorylation is required for optimal stimulation of the Arp2/3 complex by WASP...
  22. pmc The putative Arabidopsis arp2/3 complex controls leaf cell morphogenesis
    Shundai Li
    Department of Botany and Plant Sciences, University of California, Riverside, California 92521, USA
    Plant Physiol 132:2034-44. 2003
    ....
  23. ncbi request reprint Interactions of tobacco microtubule-associated protein MAP65-1b with microtubules
    Catherine Wicker-Planquart
    Laboratoire de Physiologie Cellulaire Végétale, Département Réponse et Dynamique Cellulaire, 17 rue des Martyrs, UMR 5168, CNRS CEA INRA Université Joseph Fourier, F 38054 Grenoble Cedex 9, France
    Plant J 39:126-34. 2004
    ..In vitro, NtMAP65-1b stabilizes microtubules against depolymerization induced by cold, but not against katanin-induced destabilization. The biological implications of these results are discussed...
  24. pmc Structural basis of actin sequestration by thymosin-beta4: implications for WH2 proteins
    Edward Irobi
    Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden
    EMBO J 23:3599-608. 2004
    ..The structure implies that multiple WH2 motif-containing proteins will associate longitudinally with actin filaments. Finally, we discuss the role of the WH2 motif in arp2/3 activation...
  25. ncbi request reprint Plant formin AtFH5 is an evolutionarily conserved actin nucleator involved in cytokinesis
    Mathieu Ingouff
    EMBO YIP Team, Unite Mixte de Recherche 5667, Institut Fédératif de Recherche 128 BioSciences Lyon Gerland, Ecole Normale Superieure de Lyon, 46 allee d Italie, F 69364 Lyon Cedex 07, France
    Nat Cell Biol 7:374-80. 2005
    ..Although cytokinesis in animals, yeast and plants occurs through morphologically distinct mechanisms, our study finds that formin recruitment to sites of actin assembly is a common feature of cell division among eukaryotes...
  26. pmc The formin homology 1 domain modulates the actin nucleation and bundling activity of Arabidopsis FORMIN1
    Alphée Michelot
    Laboratoire de Physiologie Cellulaire Végétale, Commissariat a l Energie Atomique, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Joseph Fourier, Unité Mixte de Recherche 5168, Grenoble, France
    Plant Cell 17:2296-313. 2005
    ..We suggest that AFH1 plays a central role in the initiation and organization of actin cables from the pool of actin monomers bound to profilin...
  27. ncbi request reprint Hydrolysis of ATP by polymerized actin depends on the bound divalent cation but not profilin
    Laurent Blanchoin
    Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 41:597-602. 2002
    ..Profilin must move from this site at rates much faster than it dissociates from monomers (4 s(-1)). ATP hydrolysis is not required for this movement...