Hypoxylon pulicicidum sp. nov. (Ascomycota, Xylariales), a pantropical insecticide-producing endophyteGerald F Bills
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Parque Tecnológico de Ciencias de la Salud, Armilla, Granada, Spain
PLoS ONE 7:e46687. 2012
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The "FERMEX" method for metabolite-enriched fungal extractsGerald F Bills
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Armilla, Granada, Spain
Methods Mol Biol 944:79-96. 2012
..The necessary equipment, protocol, and media recipes are described along with examples of bioactive fungal metabolites produced in this system...
- Kabatiella bupleuri sp. nov. (Dothideales), a pleomorphic epiphyte and endophyte of the Mediterranean plant Bupleurum gibraltarium (Apiaceae)
Gerald F Bills
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Granada, Spain
Mycologia 104:962-73. 2012
..This new species is a sister species of the morphologically similar clover pathogen, K. caulivora...
Discovery of the parnafungins, antifungal metabolites that inhibit mRNA polyadenylation, from the Fusarium larvarum complex and other Hypocrealean fungiGerald F Bills
Centro de Investigación Básica, Merck, SHARP and DOHME de ESPANA S A, Josefa Valcárcel 38, Madrid, E 28027, Spain
Mycologia 101:449-72. 2009
..rubrum represents a species distinct from var. larvarum. Finally we report that two other species from the Hypocreales, Trichonectria rectipila and Cladobotryum pinarense, are able to produce parnafungins and their open-ring forms...
- Isolation and structural elucidation of cyclic tetrapeptides from Onychocola sclerotica
Ignacio Pérez-Victoria
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avenida del Conocimiento 3, Parque Tecnológico de Ciencias de la Salud, E 18100 Armilla, Granada, Spain
J Nat Prod 75:1210-4. 2012
..2) but did not inhibit the hERG potassium channel and were not cytotoxic. These peptides are the first secondary metabolites ever reported from fungi of the order Arachnomycetales...
- Distribution of the antifungal agents sordarins across filamentous fungi
Francisca Vicente
Centro de Investigación Básica, Merck Sharp and Dohme de Espana, Josefa Valcarcel, 38, 28027 Madrid, Spain
Mycol Res 113:754-70. 2009
..e., Sordariales, Eurotiales, and Microascales), suggesting that the production of sordarin is a trait more frequently associated to members of the Xylariales than to any other fungal order...
- High-throughput culturing of fungi from plant litter by a dilution-to-extinction technique
Javier Collado
Centro de Investigación Básica, Merck Sharp and Dohme de Espana, S A, Madrid, Spain
FEMS Microbiol Ecol 60:521-33. 2007
..Intercolony interactions are reduced, permitting longer incubation times, and colony initiation and recovery improved. Effort to evaluate and recover colonies from fungal isolation plates was substantially reduced...
- Studies on Morinia: recognition of Morinia longiappendiculata sp. nov. as a new endophytic fungus, and a new circumscription of Morinia pestalozzioides
Javier Collado
Centro de Investigación Básica, Merck Research Laboratories, Merck, Sharp and Dohme de España, josefa Valcárcel 38 E 28027 Madrid, Espana
Mycologia 98:616-27. 2006
..A lectotype and epitype are designated for the species. A study of bioactive metabolites revealed that M. pestalozzioides cultures produced moriniafungin, a novel sordarin analog with potent antifungal activity...
- Manipulating filamentous fungus chemical phenotypes by growth on nutritional arrays
José R Tormo
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Armilla, Granada, Spain
Methods Mol Biol 944:59-78. 2012
..The necessary equipment, custom tools, and protocols to grow fungi in nutritional arrays are described along with examples of bioactive secondary metabolites discovered using this system...
- Conspecificity of the cerulenin and helvolic acid producing 'Cephalosporium caerulens', and the hypocrealean fungus Sarocladium oryzae
Gerald F Bills
Centro de Investigación Básica, Merck Sharp and Dohme de Espana, Josefa Valcárcel 38, ES 28027 Madrid, Spain
Mycol Res 108:1291-300. 2004
..At least two of the isolates of S. oryzae produced titres of cerulenin and helvolic acid similar to those of KF-140. This finding demonstrates that manufacture of cerulenin need not be limited to the original strain...
- Prescreening bacterial colonies for bioactive molecules with Janus plates, a SBS standard double-faced microbial culturing system
Marina Sanchez-Hidalgo
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Parque Tecnológico de Ciencias de la Salud, Armilla, Granada, Spain
Antonie Van Leeuwenhoek 102:361-74. 2012
..We also envisage the method's utility for functional prescreening colonies of clones from genomic and metagenomic libraries or improved strains originating from mutagenized cells...
- Chemical and physical modulation of antibiotic activity in emericella species
Mercedes de la Cruz
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda del Conocimiento 3, Parque Tecnológico de Ciencias de la Salud, ES 18100 Armilla, Granada
Chem Biodivers 9:1095-113. 2012
..Several species are reported for the first time to produce echinocandin B. A new echinocandin analog, echinocandin E, was identified from E. quadrilineata...
