L Best

Summary

Publications

  1. pmc A dual action of saturated fatty acids on electrical activity in rat pancreatic β-cells. Role of volume-regulated anion channel and KATP channel currents
    L Best
    Schools of Biomedicine, University of Manchester, Manchester, UK
    J Physiol 589:1307-16. 2011
  2. doi request reprint Studies of the mechanism of activation of the volume-regulated anion channel in rat pancreatic beta-cells
    Len Best
    School of Medicine, Manchester Royal Infirmary, University of Manchester, Oxford Road, Manchester M139WL, UK
    J Membr Biol 230:83-91. 2009
  3. doi request reprint Opposing effects of tenidap on the volume-regulated anion channel and K(ATP) channel activity in rat pancreatic beta-cells
    Leonard Best
    Schools of Medicine, University of Manchester, UK
    Eur J Pharmacol 629:159-63. 2010
  4. ncbi request reprint Electrophysiological effects of osmotic cell shrinkage in rat pancreatic β-cells
    Len Best
    Schools of Biomedicine, University of Manchester, Manchester, UK
    Islets 2:303-7. 2010
  5. doi request reprint Electrical activity in pancreatic islet cells: The VRAC hypothesis
    Leonard Best
    University of Manchester, UK
    Islets 2:59-64. 2010
  6. ncbi request reprint Swelling-induced changes in cytosolic [Ca2++] in insulin-secreting cells: a role in regulatory volume decrease?
    E A Sheader
    Department of Medicine, University of Manchester, M13 9WL, Manchester, UK
    Mol Cell Endocrinol 181:179-87. 2001
  7. pmc Expression of volume-sensitive Cl(-) channels and ClC-3 in acinar cells isolated from the rat lacrimal gland and submandibular salivary gland
    A Majid
    School of Biological Sciences, G.38 Stopford Building, University of Manchester, Manchester M13 9PT, UK
    J Physiol 534:409-21. 2001
  8. ncbi request reprint Glucose-sensitive conductances in rat pancreatic beta-cells: contribution to electrical activity
    L Best
    Department of Medicine, University of Manchester, Oxford Road, M13 9WL, Manchester, UK
    Biochim Biophys Acta 1468:311-9. 2000
  9. ncbi request reprint Cell-attached recordings of the volume-sensitive anion channel in rat pancreatic beta-cells
    L Best
    Department of Medicine, University of Manchester, Oxford Road, Manchester M13 9WL, UK
    Biochim Biophys Acta 1419:248-56. 1999
  10. ncbi request reprint Tolbutamide potentiates the volume-regulated anion channel current in rat pancreatic beta cells
    L Best
    Department of Medicine, University of Manchester, UK
    Diabetologia 47:1990-7. 2004

Collaborators

Detail Information

Publications17

  1. pmc A dual action of saturated fatty acids on electrical activity in rat pancreatic β-cells. Role of volume-regulated anion channel and KATP channel currents
    L Best
    Schools of Biomedicine, University of Manchester, Manchester, UK
    J Physiol 589:1307-16. 2011
    ..These effects appear to be the result of reciprocal actions on VRAC and KATP channel currents, and could contribute towards the stimulatory and inhibitory actions of FFAs on pancreatic β-cell function...
  2. doi request reprint Studies of the mechanism of activation of the volume-regulated anion channel in rat pancreatic beta-cells
    Len Best
    School of Medicine, Manchester Royal Infirmary, University of Manchester, Oxford Road, Manchester M139WL, UK
    J Membr Biol 230:83-91. 2009
    ..These findings are consistent with the hypothesis that the accumulation of glucose metabolites in the beta cell, and the resultant increase in cell volume, provides a signal coupling glucose metabolism with VRAC activation...
  3. doi request reprint Opposing effects of tenidap on the volume-regulated anion channel and K(ATP) channel activity in rat pancreatic beta-cells
    Leonard Best
    Schools of Medicine, University of Manchester, UK
    Eur J Pharmacol 629:159-63. 2010
    ..These actions could at least in part explain the recently reported inhibitory actions of the drug on electrical and secretory activity in the beta-cell, and could also underlie other pharmacological actions of the drug...
  4. ncbi request reprint Electrophysiological effects of osmotic cell shrinkage in rat pancreatic β-cells
    Len Best
    Schools of Biomedicine, University of Manchester, Manchester, UK
    Islets 2:303-7. 2010
    ..The secondary hyperpolarisation is likely to be the result of inhibition of VRAC activity. These opposing ionic effects could underlie the biphasic effect on insulin release following exposure to hypertonic media...
  5. doi request reprint Electrical activity in pancreatic islet cells: The VRAC hypothesis
    Leonard Best
    University of Manchester, UK
    Islets 2:59-64. 2010
    ....
  6. ncbi request reprint Swelling-induced changes in cytosolic [Ca2++] in insulin-secreting cells: a role in regulatory volume decrease?
    E A Sheader
    Department of Medicine, University of Manchester, M13 9WL, Manchester, UK
    Mol Cell Endocrinol 181:179-87. 2001
    ..RVD depends upon simultaneous activation of Cl(-) and K(+) channels. We suggest that the BK(Ca) channel is the major K(+) conductance involved in RVD...
  7. pmc Expression of volume-sensitive Cl(-) channels and ClC-3 in acinar cells isolated from the rat lacrimal gland and submandibular salivary gland
    A Majid
    School of Biological Sciences, G.38 Stopford Building, University of Manchester, Manchester M13 9PT, UK
    J Physiol 534:409-21. 2001
    ..5. The data suggest that the expression of ClC-3 is not an absolute requirement for the activity of volume-sensitive anion channels in rat lacrimal gland acinar cells...
  8. ncbi request reprint Glucose-sensitive conductances in rat pancreatic beta-cells: contribution to electrical activity
    L Best
    Department of Medicine, University of Manchester, Oxford Road, M13 9WL, Manchester, UK
    Biochim Biophys Acta 1468:311-9. 2000
    ..At higher glucose concentrations effective in generating electrical activity, activation of the volume-sensitive anion channel could contribute towards the nutrient-induced increase in G(input)...
  9. ncbi request reprint Cell-attached recordings of the volume-sensitive anion channel in rat pancreatic beta-cells
    L Best
    Department of Medicine, University of Manchester, Oxford Road, Manchester M13 9WL, UK
    Biochim Biophys Acta 1419:248-56. 1999
    ..This generates an inward current in non-voltage-clamped cells. The channel showed complex kinetics which depended in part upon extracellular [Cl(-)]...
  10. ncbi request reprint Tolbutamide potentiates the volume-regulated anion channel current in rat pancreatic beta cells
    L Best
    Department of Medicine, University of Manchester, UK
    Diabetologia 47:1990-7. 2004
    ..The aim of this study was to test the hypothesis that sulphonylureas influence the current of the glucose- and volume-regulated anion channel...
  11. ncbi request reprint How does glucose evoke electrical activity in the pancreatic beta-cell?
    L Best
    Department of Medicine, University of Manchester, Oxford Road, Manchester, M13 9WL, UK
    Diabetes Metab 28:3S18-24; discussion 3S108-12. 2002
    ..It is possible that the major role of the K(ATP) channel is in hyperpolarizing the cell, thus inhibiting electrical and secretory activity, during hypoglycaemia...
  12. pmc Glucose-induced electrical activity in rat pancreatic beta-cells: dependence on intracellular chloride concentration
    L Best
    Department of Medicine, University of Manchester, Multipurpose Building, Manchester Royal Infirmary, Oxford Road, Manchester, M13 9WL, UK
    J Physiol 568:137-44. 2005
    ....
  13. ncbi request reprint Evidence that glucose-induced electrical activity in rat pancreatic beta-cells does not require KATP channel inhibition
    L Best
    Department of Medicine, Manchester Royal Infirmary, University of Manchester, Oxford Road, Manchester M13 9WL, UK
    J Membr Biol 185:193-200. 2002
    ..The KATP channel-independent mechanism could involve, at least in part, activation by glucose of the volume-sensitive anion channel...
  14. ncbi request reprint Expression of the Na+K+-2CI- cotransporter in alpha and beta cells isolated from the rat pancreas
    A Majid
    School of Biological Sciences and Department of Medicine, University of Manchester, UK
    Pflugers Arch 442:570-6. 2001
    ..This is consistent with the hypothesis that Cl- is accumulated above the expected equilibrium distribution in beta cells, but is below equilibrium in alpha cells...
  15. ncbi request reprint Functional characterisation of the volume-sensitive anion channel in rat pancreatic beta-cells
    L Best
    Department of Medicine, University of Manchester, Oxford Road, Manchester M13 9WL, UK
    Exp Physiol 86:145-50. 2001
    ..Furthermore, the beta-cell channel does not appear to be subject to regulation via protein kinase C. Experimental Physiology (2001) 86.2, 145-150...
  16. ncbi request reprint Cytotoxic action of methylglyoxal on insulin-secreting cells
    E A Sheader
    Department of Medicine, Manchester Royal Infirmary, Manchester University, Oxford Road, M13 9WL, Manchester, UK
    Biochem Pharmacol 61:1381-6. 2001
    ..Flow cytometric analysis suggested a degree of necrosis in addition to apoptosis resulting from treatment with methylglyoxal. The cytotoxic effect of methylglyoxal could contribute towards glucose toxicity in insulin-secreting cells...
  17. ncbi request reprint Trioses and related substances: tools for the study of pancreatic beta-cell function
    L Best
    Department of Medicine, University of Manchester, England, UK
    Biochem Pharmacol 57:583-8. 1999
    ..These substances are also likely to be of pathophysiological importance, especially in the context of sugar toxicity and autoxidative cell damage...