MicroRNAs in metamorphic and non-metamorphic transitions in hemimetabolan insect metamorphosisMercedes Rubio
Institute of Evolutionary Biology, CSIC UPF, Passeig Marítim 39, 08003 Barcelona, Spain
BMC Genomics 13:386. 2012
..To gain insight about which miRNAs might be important, we have constructed two miRNA libraries, one of the penultimate, pre-metamorphic nymphal instar (N5) and the other of the last, metamorphic nymphal instar (N6)...
Beyond Drosophila: RNAi in vivo and functional genomics in insectsXavier Belles
Institut de Biologia Evolutiva CSIC UPF, Barcelona, Spain
Annu Rev Entomol 55:111-28. 2010
..RNAi also has drawbacks and obscure points, however, such as those related to differences in species sensitivity. Disentangling these differences is one of the main challenges in the RNAi field...
- The hormonal pathway controlling cell death during metamorphosis in a hemimetabolous insect
Daniel Mané-Padrós
Institute of Evolutionary Biology CSIC UPF, Passeig Marítim de la Barceloneta 37 49, 08003 Barcelona, Spain
Dev Biol 346:150-60. 2010
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- Nuclear receptor HR4 plays an essential role in the ecdysteroid-triggered gene cascade in the development of the hemimetabolous insect Blattella germanica
Daniel Mané-Padrós
Institute of Evolutionary Biology CSIC UPF, Passeig Marítim de la Barceloneta 37 49, 08003 Barcelona, Spain
Mol Cell Endocrinol 348:322-30. 2012
..Finally, we have also shown that BgHR4 is an essential gene required for successfully completing nymphal-nymphal and nymphal-adult transitions, by allowing the appropriate delay in the induction of BgFTZ-F1...
- Identification and functional characterization of an ovarian aquaporin from the cockroach Blattella germanica L. (Dictyoptera, Blattellidae)
Alba Herraiz
Institut de Biologia Evolutiva CSIC UPF and LINC Global, Passeig Maryítim de la Barceloneta 37 49, 08003 Barcelona, Spain
J Exp Biol 214:3630-8. 2011
..The absence of an apparent ovarian phenotype after BgAQP RNAi suggests that other functionally redundant AQPs that were not silenced in our experiments might exist in the ovary of B. germanica...
- Key roles of the Broad-Complex gene in insect embryogenesis
Maria Dolors Piulachs
Institut de Biologia Evolutiva CSIC UPF, Passeig Marítim 39, 08003 Barcelona, Spain
Insect Biochem Mol Biol 40:468-75. 2010
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- Brownie, a gene involved in building complex respiratory devices in insect eggshells
Paula Irles
Institut de Biologia Evolutiva UPF CSIC, Barcelona, Spain
PLoS ONE 4:e8353. 2009
..The sponge-like body has been well described morphologically, but nothing is known about how it is built up...
- Target of rapamycin (TOR) mediates the transduction of nutritional signals into juvenile hormone production
José L Maestro
Institut de Biologia Evolutiva CSIC UPF, Passeig Marítim de la Barceloneta 37 49, 08003 Barcelona, Spain
J Biol Chem 284:5506-13. 2009
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- Conservation of fruitless' role as master regulator of male courtship behaviour from cockroaches to flies
Elke Clynen
Institute of Evolutionary Biology CSIC UPF, Passeig Maritim de la Barceloneta 37, 08003, Barcelona, Spain
Dev Genes Evol 221:43-8. 2011
..This suggests that the role of fruitless as master regulator of male sexual behaviour has been conserved along insect evolution, at least from cockroaches to flies...
- Leucomyosuppressin modulates cardiac rhythm in the cockroach Blattella germanica
José L Maestro
Institut de Biologia Evolutiva CSIC UPF, Passeig Maritim de la Barceloneta 37, 08003 Barcelona, Spain
J Insect Physiol 57:1677-81. 2011
..These data suggest that LMS contributes to the modulation of cardiac rhythm in B. germanica during the reproductive cycle...
- Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptors
Laura Ciudad
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona, CSIC, Jordi Girona 18, Barcelona, Spain
BMC Mol Biol 8:53. 2007
..The aim of the present work was to characterize the LpR of the cockroach Blattella germanica, not only molecularly but also functionally using RNAi techniques, and to place LpRs in a phylogenetical context among lipoprotein receptors...
Effects of hypocholesterolaemic agents on the expression and activity of 3-hydroxy-3-methylglutaryl-CoA reductase in the fat body of the German cockroachRafael Zapata
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona, Barcelona, Spain
Arch Insect Biochem Physiol 49:177-86. 2002
..Short-term assays showed that both compounds reduce the protein levels and enzymatic activity of HMG-CoA reductase, and long-term experiments revealed that fluvastatin impairs embryo development...
- Super-induction of Dicer-2 expression by alien double-stranded RNAs: an evolutionary ancient response to viral infection?
Jesus Lozano
Institute of Evolutionary Biology, Barcelona, Spain
Dev Genes Evol 222:229-35. 2012
..germanica, a phylogenetically basal insect, suggests that sensing alien RNAs might be an ancestral function of Dicer-2 proteins...
- Juvenile hormone and allatostatins in the German cockroach embryo
José L Maestro
Institut de Biologia Evolutiva CSIC UPF, Passeig Marítim de la Barceloneta 37 49, 08003 Barcelona, Spain
Insect Biochem Mol Biol 40:660-5. 2010
..Moreover, the uncorrelated patterns of JH concentration and CYP15A1 mRNA levels suggest that CYP15A1 expression does not modulate JH production...
- Systemic RNAi of the cockroach vitellogenin receptor results in a phenotype similar to that of the Drosophila yolkless mutant
Laura Ciudad
Department of Physiology and Molecular Biodiversity. Institute of Molecular Biology of Barcelona, Barcelona, Spain
FEBS J 273:325-35. 2006
..The results additionally open the perspective that development genes can be functionally analyzed via systemic RNAi in this basal species...
- MicroRNA-dependent metamorphosis in hemimetabolan insects
Eva Gómez-Orte
Institut de Biologia Evolutiva Consejo Superior de Investigaciones Científicas and Universitat Pompeu Fabra, Passeig Maritim de la Barceloneta, 37 49, 08003 Barcelona, Spain
Proc Natl Acad Sci U S A 106:21678-82. 2009
..The experiments show that miRNAs play a key role in hemimetabolan metamorphosis, perhaps regulating genes that are juvenile hormone targets...
- Nuclear receptor BgFTZ-F1 regulates molting and the timing of ecdysteroid production during nymphal development in the hemimetabolous insect Blattella germanica
Josefa Cruz
Institut de Biologia Evolutiva CSIC UPF, Passeig Marítim de la Barceloneta 37 49, Barcelona, Spain
Dev Dyn 237:3179-91. 2008
..Nymphs with silenced BgFTZ-F1 cannot ecdyse, arrest development, and show structures of ectodermal origin duplicated. BgFTZ-F1 also controls the timing of the ecdysteroid molting pulse...
- Broad-complex functions in postembryonic development of the cockroach Blattella germanica shed new light on the evolution of insect metamorphosis
Jia Hsin Huang
CSIC Universitat Pompeu Fabra, Passeig Maritim de la Barceloneta, Barcelona, Spain
Biochim Biophys Acta 1830:2178-87. 2013
..In holometabolous species, like Drosophila melanogaster, BR-C expression is inhibited by JH in young larvae and enhanced in mature larvae, when JH declines and BR-C expression specifies the pupal stage...
- Conserved repressive function of Krüppel homolog 1 on insect metamorphosis in hemimetabolous and holometabolous species
Jesus Lozano
Institut de Biologia Evolutiva CSIC UPF, Passeig Martim de la Barceloneta 37, 08003 Barcelona, Spain
Sci Rep 1:163. 2011
..germanica, thereby suggesting that this role is an ancestral condition that has been conserved in insect evolution from hemimetabolous to holometabolous species...
- Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization
Paula Irles
Institut de Biologia Evolutiva UPF CSIC, Passeig Maritim de la Barceloneta, Barcelona, Spain
BMC Genomics 10:206. 2009
..To this end, we planned the construction of a Suppression Subtractive Hybridization (SSH) library to identify genes involved in panoistic choriogenesis, using the cockroach Blattella germanica as model...
- Identification of a tachykinin-related peptide with orexigenic properties in the German cockroach
Nuria Pascual
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona CSIC, Jordi Girona 18 26, Barcelona, Spain
Peptides 29:386-92. 2008
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- Silencing allatostatin expression using double-stranded RNA targeted to preproallatostatin mRNA in the German cockroach
José L Maestro
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona, CSIC, Barcelona, Spain
Arch Insect Biochem Physiol 62:73-9. 2006
..Nevertheless, while brain AST levels were reduced approximately 70-80%, JH synthesis did not increase in any of the age groups tested...
