Patrick M L Vanderheyden

Summary

Affiliation: Vrije Universiteit Brussel
Country: Belgium

Publications

  1. ncbi request reprint Effect of BIBP3226 on inositol phosphate accumulation and cytosolic calcium level in control and NPY Y1 receptor expressing CHO-K1 cells
    P M Vanderheyden
    Department of Protein Chemistry, Free University of Brussels VUB, Sint Genesius Rode, Belgium
    Regul Pept 75:191-9. 1998
  2. ncbi request reprint Binding characteristics of [(3)H]-irbesartan to human recombinant angiotensin type 1 receptors
    P M Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Free University of Brussels, Rode, B 1640, Belgium
    J Renin Angiotensin Aldosterone Syst 1:159-65. 2000
  3. ncbi request reprint Synergistic inhibition of the enzymatic activity of aminopeptidase N by divalent metal ion chelators
    Patrick M L Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Fundam Clin Pharmacol 20:613-9. 2006
  4. doi request reprint From angiotensin IV binding site to AT4 receptor
    Patrick M L Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    Mol Cell Endocrinol 302:159-66. 2009
  5. ncbi request reprint Inhibition of angiotensin II-induced inositol phosphate production by triacid nonpeptide antagonists in CHO cells expressing human AT1 receptors
    P M Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Institute of Molecular Biology and Biotechnology, Free University of Brussels, Sint Genesius Rode, Belgium
    Pharm Res 17:1482-8. 2000
  6. ncbi request reprint Angiotensin II type 1 receptor antagonists. Why do some of them produce insurmountable inhibition?
    P M Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Free University of Brussels, Sint Genesius Rode, Belgium
    Biochem Pharmacol 60:1557-63. 2000
  7. ncbi request reprint Reversible and syntopic interaction between angiotensin receptor antagonists on Chinese hamster ovary cells expressing human angiotensin II type 1 receptors
    P M Vanderheyden
    Department of Molecular Pharmacology, Free University of Brussels, Sint Genesius Rode, Belgium
    Biochem Pharmacol 59:927-35. 2000
  8. pmc Distinction between surmountable and insurmountable selective AT1 receptor antagonists by use of CHO-K1 cells expressing human angiotensin II AT1 receptors
    P M Vanderheyden
    Department of Protein Chemistry, Free University of Brussels VUB, Sint Genesius Rode, Belgium
    Br J Pharmacol 126:1057-65. 1999
  9. pmc Metal ion modulation of cystinyl aminopeptidase
    Hilde Laeremans
    Department of Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Biochem J 390:351-7. 2005
  10. ncbi request reprint Ligand binding and functional properties of human angiotensin AT1 receptors in transiently and stably expressed CHO-K1 cells
    Minh Tam Le
    Department of Molecular and Biochemical Pharmacology, Institute for Molecular Biology and Biotechnology, Vrije Universiteit Brussel VUB, Belgium
    Eur J Pharmacol 513:35-45. 2005

Collaborators

Detail Information

Publications20

  1. ncbi request reprint Effect of BIBP3226 on inositol phosphate accumulation and cytosolic calcium level in control and NPY Y1 receptor expressing CHO-K1 cells
    P M Vanderheyden
    Department of Protein Chemistry, Free University of Brussels VUB, Sint Genesius Rode, Belgium
    Regul Pept 75:191-9. 1998
    ..This effect of BIBP3226 is likely to be mediated by activation of an until now unknown receptor or cellular target that is endogeneously expressed in CHO-K1 cells...
  2. ncbi request reprint Binding characteristics of [(3)H]-irbesartan to human recombinant angiotensin type 1 receptors
    P M Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Free University of Brussels, Rode, B 1640, Belgium
    J Renin Angiotensin Aldosterone Syst 1:159-65. 2000
    ..In contrast, other phenomena such as the plasma half life and tissue-related factors are necessary to explain its sustained in vivo antihypertensive effect...
  3. ncbi request reprint Synergistic inhibition of the enzymatic activity of aminopeptidase N by divalent metal ion chelators
    Patrick M L Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Fundam Clin Pharmacol 20:613-9. 2006
    ..Compatible with this model, Ca2+ may bind to this allosteric site resulting in the potentiation of Zn2+-mediated re-activation of the enzyme activity in the presence of EDTA and 1,10-phenanthroline...
  4. doi request reprint From angiotensin IV binding site to AT4 receptor
    Patrick M L Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    Mol Cell Endocrinol 302:159-66. 2009
    ..This link is discussed for the AT(4) receptor by providing an overview of the cellular effects by AT(4) ligands...
  5. ncbi request reprint Inhibition of angiotensin II-induced inositol phosphate production by triacid nonpeptide antagonists in CHO cells expressing human AT1 receptors
    P M Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Institute of Molecular Biology and Biotechnology, Free University of Brussels, Sint Genesius Rode, Belgium
    Pharm Res 17:1482-8. 2000
    ..The aim of the present work is to describe the inhibitory properties of LY301875 and LY303336, two polysubstituted 4-aminoimidazole AT1 receptor antagonists, on CHO cells expressing human recombinant AT1 receptors...
  6. ncbi request reprint Angiotensin II type 1 receptor antagonists. Why do some of them produce insurmountable inhibition?
    P M Vanderheyden
    Department of Molecular and Biochemical Pharmacology, Free University of Brussels, Sint Genesius Rode, Belgium
    Biochem Pharmacol 60:1557-63. 2000
    ..In addition to the relatively slow dissociation of candesartan, reassociation to the receptor, which is measurable in CHO-AT(1) cells, likely contributes to its long-lasting blood pressure lowering effect in vivo...
  7. ncbi request reprint Reversible and syntopic interaction between angiotensin receptor antagonists on Chinese hamster ovary cells expressing human angiotensin II type 1 receptors
    P M Vanderheyden
    Department of Molecular Pharmacology, Free University of Brussels, Sint Genesius Rode, Belgium
    Biochem Pharmacol 59:927-35. 2000
    ..In addition, similar kinetic data were obtained from the slowing of the [(3)H]candesartan association rate to antagonist preincubated cells...
  8. pmc Distinction between surmountable and insurmountable selective AT1 receptor antagonists by use of CHO-K1 cells expressing human angiotensin II AT1 receptors
    P M Vanderheyden
    Department of Protein Chemistry, Free University of Brussels VUB, Sint Genesius Rode, Belgium
    Br J Pharmacol 126:1057-65. 1999
    ....
  9. pmc Metal ion modulation of cystinyl aminopeptidase
    Hilde Laeremans
    Department of Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Biochem J 390:351-7. 2005
    ..These findings support the concept that high-affinity [125I]angiotensin IV binding, previously referred to as 'AT4 receptor binding', only occurs for the cystinyl aminopeptidase apoenzyme...
  10. ncbi request reprint Ligand binding and functional properties of human angiotensin AT1 receptors in transiently and stably expressed CHO-K1 cells
    Minh Tam Le
    Department of Molecular and Biochemical Pharmacology, Institute for Molecular Biology and Biotechnology, Vrije Universiteit Brussel VUB, Belgium
    Eur J Pharmacol 513:35-45. 2005
    ..Confocal microscopy revealed rapid internalization induced by angiotensin II and sarile but not by candesartan. The above disparities may result from differences in receptor maturation and/or cellular surrounding...
  11. ncbi request reprint Peptide and nonpeptide antagonist interaction with constitutively active human AT1 receptors
    Minh Tam Le
    Department of Molecular and Biochemical Pharmacology, Institute for Molecular Biology and Biotechnology, Vrije Universiteit Brussel VUB, Pleinlaan 2, Belgium
    Biochem Pharmacol 65:1329-38. 2003
    ....
  12. ncbi request reprint Synergistic modulation of cystinyl aminopeptidase by divalent cation chelators
    Heidi Demaegdt
    Research Group on Experimental Pharmacology, Department of Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium
    Biochem Pharmacol 68:893-900. 2004
    ..Modulation of the effects of 1,10-PHE by other chelators such as EDTA or EGTA, suggests that, in addition to the binding site for zinc in the catalytic site, cystinyl aminopeptidase also bears a regulatory divalent cation binding site...
  13. ncbi request reprint Antagonist interaction with endogenous AT(1) receptors in human cell lines
    Ilse Verheijen
    Department of Molecular and Biochemical Pharmacology, Free University of Brussels VUB, 65 Paardenstraat, B 1640 Sint Genesius Rode, Belgium
    Biochem Pharmacol 64:1207-14. 2002
    ..The similar binding and inhibitory properties of these antagonists among the investigated cell types validates the use of CHO-hAT(1) cells for investigating pharmacological properties of human AT(1) receptors...
  14. ncbi request reprint Effect of saponin and filipin on antagonist binding to AT 1 receptors in intact cells
    Ilse Verheijen
    Department of Molecular and Biochemical Pharmacology, Free University of Brussels VUB, Pleinlaan 2, 1050 Brussels, Belgium
    Biochem Pharmacol 67:1601-6. 2004
    ..This suggests that the intracellular composition and/or organisation of living cells play an active role with regard to antagonist-AT1 receptor interactions...
  15. ncbi request reprint Distinct binding properties of the AT(1) receptor antagonist [(3)H]candesartan to intact cells and membrane preparations
    Frederik L P Fierens
    Department of Molecular and Biochemical Pharmacology, Free University of Brussels VUB, 65 Paardenstraat, B 1640 Sint Genesius Rode, Belgium
    Biochem Pharmacol 63:1273-9. 2002
    ..Whereas the binding was almost completely enthalpy-driven on intact cells, there was a mixed contribution of both enthalpy and entropy on membranes...
  16. doi request reprint [3H]IVDE77, a novel radioligand with high affinity and selectivity for the insulin-regulated aminopeptidase
    Alexandros Nikolaou
    Molecular and Biochemical Pharmacology, Vrije Universiteit Brussel, Building E, Pleinlaan 2, B 1050 Brussels, Belgium
    Eur J Pharmacol 702:93-102. 2013
    ..In summary, IVDE77 is a useful tool for the detection of IRAP under physiological conditions, and may contribute to elucidating the mechanism of Ang IV to ascertain which functions are IRAP-dependent...
  17. doi request reprint Antagonist-radioligand binding to D2L-receptors in intact cells
    Ann Packeu
    Free University of Brussels VUB, Department of Molecular and Biochemical Pharmacology, Institute for Molecular Biology and Biotechnology, Pleinlaan 2, B 1050 Brussels, Belgium
    Biochem Pharmacol 75:2192-203. 2008
    ..To integrate these new findings, a model is proposed in which raclopride approaches the receptor from the aqueous phase, while spiperone approaches the receptor by lateral diffusion within the membrane...
  18. ncbi request reprint Molecular characterization of the high-affinity [3H]neuropeptide Y-binding component from the venom of Conus anemone
    Minh Tam Le
    Department of Molecular and Biochemical Pharmacology, Institute for Molecular Biology and Biotechnology, Free University of Brussels VUB, Pleinlaan 2, 1050 Brussel, Belgium
    Fundam Clin Pharmacol 17:457-62. 2003
    ..Its approximate molecular weight is 17.5 kDa and its [3H]NPY binding activity is extremely stable below 37 degrees C, even in the absence of protease inhibitors...
  19. ncbi request reprint Angiotensin IV is a potent agonist for constitutive active human AT1 receptors. Distinct roles of the N-and C-terminal residues of angiotensin II during AT1 receptor activation
    Minh Tam Le
    Department of Molecular and Biochemical Pharmacology, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, B 1640 Sint Genesius Rode, Belgium
    J Biol Chem 277:23107-10. 2002
    ..The receptor adopts a more relaxed conformation, allowing the binding of the C-terminal five residues of Ang II that switches this "preactivated" receptor into the fully active conformation...
  20. ncbi request reprint Formation of angiotensin-(1-7) from angiotensin II by the venom of Conus geographus
    Minh Tam Le
    Department of Molecular and Biochemical Pharmacology, Institute for Molecular Biology and Biotechnology, Free University of Brussels VUB, Paardenstraat 65, B 1640 Sint Genesius Rode, Belgium
    Regul Pept 105:101-8. 2002
    ..The molecular weight of the involved peptidases exceeds 50 kDa, as determined by gel chromatography and ultrafitration...