Benoit Stijlemans

Summary

Affiliation: Vrije Universiteit Brussel
Country: Belgium

Publications

  1. doi Role of iron homeostasis in trypanosomiasis-associated anemia
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel VUB, Building E, Level 8, Pleinlaan 2, B 1050 Brussels, Belgium
    Immunobiology 213:823-35. 2008
  2. pmc Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly
    Linda De Vooght
    Department of Biomedical Sciences, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium
    Microb Cell Fact 11:23. 2012
  3. pmc High affinity nanobodies against the Trypanosome brucei VSG are potent trypanolytic agents that block endocytosis
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Brussels, Belgium
    PLoS Pathog 7:e1002072. 2011
  4. doi Scrutinizing the mechanisms underlying the induction of anemia of inflammation through GPI-mediated modulation of macrophage activation in a model of African trypanosomiasis
    Benoit Stijlemans
    Department of Molecular and Cellular Interactions, VIB, 1050 Brussel, Belgium
    Microbes Infect 12:389-99. 2010
  5. ncbi The central role of macrophages in trypanosomiasis-associated anemia: rationale for therapeutical approaches
    Benoit Stijlemans
    Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium
    Endocr Metab Immune Disord Drug Targets 10:71-82. 2010
  6. ncbi A glycosylphosphatidylinositol-based treatment alleviates trypanosomiasis-associated immunopathology
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Pleinlaan 2, Brussels, Belgium
    J Immunol 179:4003-14. 2007
  7. ncbi African trypanosomosis: from immune escape and immunopathology to immune intervention
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Vet Parasitol 148:3-13. 2007
  8. ncbi Parallel selection of multiple anti-infectome Nanobodies without access to purified antigens
    Dirk Saerens
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Pleinlaan 2, B 1050 Brussels, Belgium
    J Immunol Methods 329:138-50. 2008
  9. doi Identification of a parasitic immunomodulatory protein triggering the development of suppressive M1 macrophages during African trypanosomiasis
    Julio Gomez-Rodriguez
    Department of Molecular and Cellular Interactions, Vlaams Institute voor Biotechnologie VIB, Vrije Universiteit Brussel, 1050 Brussels, Belgium
    J Infect Dis 200:1849-60. 2009
  10. ncbi Experimental therapy of African trypanosomiasis with a nanobody-conjugated human trypanolytic factor
    Toya Nath Baral
    Department of Cellular and Molecular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Laboratorium voor Cellulaire en Moleculaire Immunologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
    Nat Med 12:580-4. 2006

Collaborators

Detail Information

Publications27

  1. doi Role of iron homeostasis in trypanosomiasis-associated anemia
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel VUB, Building E, Level 8, Pleinlaan 2, B 1050 Brussels, Belgium
    Immunobiology 213:823-35. 2008
    ....
  2. pmc Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly
    Linda De Vooght
    Department of Biomedical Sciences, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium
    Microb Cell Fact 11:23. 2012
    ..glossinidius as a paratransgenic platform organism in tsetse flies, few potential effector molecules have been identified so far and to date none of these molecules have been successfully expressed in this bacterium...
  3. pmc High affinity nanobodies against the Trypanosome brucei VSG are potent trypanolytic agents that block endocytosis
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Brussels, Belgium
    PLoS Pathog 7:e1002072. 2011
    ....
  4. doi Scrutinizing the mechanisms underlying the induction of anemia of inflammation through GPI-mediated modulation of macrophage activation in a model of African trypanosomiasis
    Benoit Stijlemans
    Department of Molecular and Cellular Interactions, VIB, 1050 Brussel, Belgium
    Microbes Infect 12:389-99. 2010
    ..Collectively, our results demonstrate that reprogramming macrophages towards an anti-inflammatory state alleviates anemia of inflammation by normalizing iron homeostasis and restoring erythropoiesis...
  5. ncbi The central role of macrophages in trypanosomiasis-associated anemia: rationale for therapeutical approaches
    Benoit Stijlemans
    Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium
    Endocr Metab Immune Disord Drug Targets 10:71-82. 2010
    ....
  6. ncbi A glycosylphosphatidylinositol-based treatment alleviates trypanosomiasis-associated immunopathology
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Pleinlaan 2, Brussels, Belgium
    J Immunol 179:4003-14. 2007
    ....
  7. ncbi African trypanosomosis: from immune escape and immunopathology to immune intervention
    Benoit Stijlemans
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Vet Parasitol 148:3-13. 2007
    ..Finally, the possibility to interfere with such activation states hereby providing new therapeutical modalities in the treatment of this infectious disease will be illustrated...
  8. ncbi Parallel selection of multiple anti-infectome Nanobodies without access to purified antigens
    Dirk Saerens
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Pleinlaan 2, B 1050 Brussels, Belgium
    J Immunol Methods 329:138-50. 2008
    ..Due to the omnipresence of these targets on different parasite species and forms, these antibody fragments are a valuable source for validation of novel, not yet identified targets to design new diagnostics and therapeutics...
  9. doi Identification of a parasitic immunomodulatory protein triggering the development of suppressive M1 macrophages during African trypanosomiasis
    Julio Gomez-Rodriguez
    Department of Molecular and Cellular Interactions, Vlaams Institute voor Biotechnologie VIB, Vrije Universiteit Brussel, 1050 Brussels, Belgium
    J Infect Dis 200:1849-60. 2009
    ..TSIF knock-down trypanosomes died within 2 days, indicating that TSIF may be essential for parasite biology...
  10. ncbi Experimental therapy of African trypanosomiasis with a nanobody-conjugated human trypanolytic factor
    Toya Nath Baral
    Department of Cellular and Molecular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Laboratorium voor Cellulaire en Moleculaire Immunologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
    Nat Med 12:580-4. 2006
    ..Treatment with this engineered conjugate resulted in clear curative and alleviating effects on acute and chronic infections of mice with both NHS-resistant and NHS-sensitive trypanosomes...
  11. ncbi [Experimental therapy of African trypanosomiasis with a nanobody-conjugated human trypanolytic factor]
    Toya Nath Baral
    Département des Interactions cellulaires et moléculaires, Vlaams Interuniversitair Instituut voor Biotechnologie, Laboratorium voor Cellulaire en Moleculaire Immunologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Bruxelles, Belgique
    Med Sci (Paris) 22:914-6. 2006
  12. doi Understanding the role of monocytic cells in liver inflammation using parasite infection as a model
    Tom Bosschaerts
    Department of Molecular and Cellular Interactions, VIB, Brussel, Belgium
    Immunobiology 214:737-47. 2009
    ....
  13. ncbi P75 tumor necrosis factor-receptor shedding occurs as a protective host response during African trypanosomiasis
    Stefan Magez
    Laboratory of Cellular and Molecular Immunology, Department of Cellular and Molecular Recognition, Flanders Interuniversity Institute for Biotechnology, Free University of Brussels VUB, Brussels, Belgium
    J Infect Dis 189:527-39. 2004
    ....
  14. pmc Tsetse fly saliva accelerates the onset of Trypanosoma brucei infection in a mouse model associated with a reduced host inflammatory response
    Guy Caljon
    Unit of Cellular and Molecular Immunology, Flanders Interuniversity Institute for Biotechnology VIB, Vrije Universiteit Brussel VUB, Pleinlaan 2, B 1050 Brussels, Belgium
    Infect Immun 74:6324-30. 2006
    ..We propose that tsetse fly saliva accelerates the onset of trypanosome infection by inhibiting local and systemic inflammatory responses involved in parasite control...
  15. pmc Tip-DC development during parasitic infection is regulated by IL-10 and requires CCL2/CCR2, IFN-gamma and MyD88 signaling
    Tom Bosschaerts
    Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium
    PLoS Pathog 6:e1001045. 2010
    ..Therefore, investigating the contribution of recruited monocytes to African trypanosome induced liver injury could potentially identify new targets to treat hepatic inflammation in general, and during parasite infection in particular...
  16. doi IL-10 limits production of pathogenic TNF by M1 myeloid cells through induction of nuclear NF-κB p50 member in Trypanosoma congolense infection-resistant C57BL/6 mice
    Tom Bosschaerts
    Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, Brussels, Belgium
    Eur J Immunol 41:3270-80. 2011
    ..congolense infection in resistant C57BL/6 mice...
  17. doi Lack of galectin-3 alleviates trypanosomiasis-associated anemia of inflammation
    Ann Vankrunkelsven
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
    Immunobiology 215:833-41. 2010
    ..Our data indicate that Gal-3 is involved in the development of inflammation-associated anemia during African trypanosomiasis, possibly due to a disturbed iron metabolism that in turn may also lead to liver malfunction...
  18. ncbi Efficient targeting of conserved cryptic epitopes of infectious agents by single domain antibodies. African trypanosomes as paradigm
    Benoit Stijlemans
    Department of Cellular and Molecular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    J Biol Chem 279:1256-61. 2004
    ..This was illustrated by the specific trypanolysis induced by an antibody::beta-lactamase fusion activating a prodrug...
  19. pmc Affinity is an important determinant of the anti-trypanosome activity of nanobodies
    Guy Caljon
    Department of Biomedical Sciences, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium
    PLoS Negl Trop Dis 6:e1902. 2012
    ..The anti-trypanosomal activity relies on perturbing the highly active recycling of the Variant-specific Surface Glycoprotein (VSG) that occurs in the parasite's flagellar pocket...
  20. doi Current status of vaccination against African trypanosomiasis
    Stefan Magez
    Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel VUB, Pleinlaan 2, B 1050 Brussels, Belgium
    Parasitology 137:2017-27. 2010
    ..This paper reviews past and current attempts to design both anti-trypanosome vaccines, as well as vaccines directed towards the inhibition of infection-associated pathology...
  21. pmc Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity
    Guy Caljon
    Department of Biomedical Sciences, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp ITM, Antwerp, Belgium
    PLoS ONE 7:e47233. 2012
    ..Knockdown of Tsal expression by in vivo RNA interference in the tsetse fly revealed a partially impaired blood digestion phenotype as evidenced by higher gut nucleic acid, hematin and protein contents...
  22. pmc Bacterial lipoprotein-based vaccines induce tumor necrosis factor-dependent type 1 protective immunity against Leishmania major
    Javier Cote-Sierra
    Department of Immunology, Parasitology and Ultrastructure, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Sint Genesius Rode, Belgium
    Infect Immun 70:240-8. 2002
    ..These data indicate that bacterial lipoproteins constitute appropriate adjuvants to include in vaccines against diseases in which type 1 immune responses are important for protection...
  23. ncbi Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VH
    Katja Conrath
    Laboratorium voor Cellulaire en Moleculaire Immunologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
    J Mol Biol 350:112-25. 2005
    ....
  24. pmc Origin and functional diversification of an amphibian defense peptide arsenal
    Kim Roelants
    Amphibian Evolution Lab, Biology Department, Vrije Universiteit Brussel, Brussels, Belgium
    PLoS Genet 9:e1003662. 2013
    ..The processing of multiple peptides from a single precursor entails a mechanism through which peptide-encoding genes may establish new functions without the need for gene duplication to avoid adaptive conflicts with older ones. ..
  25. pmc Low structural variation in the host-defense peptide repertoire of the dwarf clawed frog Hymenochirus boettgeri (Pipidae)
    Severine Matthijs
    Amphibian Evolution Lab, Biology Department, Vrije Universiteit Brussel, Brussels, Belgium
    PLoS ONE 9:e86339. 2014
    ..Our study demonstrates that defense peptide repertoires of closely related taxa, after diverging from each other, may evolve under differential selective regimes, leading to contrasting patterns of structural diversity...
  26. ncbi VSG-GPI anchors of African trypanosomes: their role in macrophage activation and induction of infection-associated immunopathology
    Stefan Magez
    Laboratory of Cellular Immunology, Free University of Brussels Flemish Interuniversity, Institute for Biotechnology, Paardenstraat 65, Sint Genesius Rode, Belgium
    Microbes Infect 4:999-1006. 2002
    ..Their interaction with various cells of the immune system underlies the severe infection-associated pathology. Recent results have shown that anti-GPI vaccination can prevent the occurrence of this pathology...
  27. pmc Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice
    Hannelie Korf
    Laboratory of Mycobacterial Immunology, Scientific Institute of Public Health, Brussels, Belgium
    J Clin Invest 119:1626-37. 2009
    ..The dependence of LXR signaling on the neutrophil IL-17 axis represents what we believe to be a novel function for these nuclear receptors in resistance to M. tuberculosis infection and may provide a new target for therapeutics...