Research Topics
Genomes and Genes | Benoit StijlemansSummaryAffiliation: Vrije Universiteit Brussel Country: Belgium Publications
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Detail Information
Publications
Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse flyLinda De Vooght
Department of Biomedical Sciences, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium
Microb Cell Fact 11:23. 2012..glossinidius as a paratransgenic platform organism in tsetse flies, few potential effector molecules have been identified so far and to date none of these molecules have been successfully expressed in this bacterium...- High affinity nanobodies against the Trypanosome brucei VSG are potent trypanolytic agents that block endocytosisBenoit Stijlemans
Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Brussels, Belgium
PLoS Pathog 7:e1002072. 2011.... 
Scrutinizing the mechanisms underlying the induction of anemia of inflammation through GPI-mediated modulation of macrophage activation in a model of African trypanosomiasisBenoit Stijlemans
Department of Molecular and Cellular Interactions, VIB, 1050 Brussel, Belgium
Microbes Infect 12:389-99. 2010..Collectively, our results demonstrate that reprogramming macrophages towards an anti-inflammatory state alleviates anemia of inflammation by normalizing iron homeostasis and restoring erythropoiesis...
The central role of macrophages in trypanosomiasis-associated anemia: rationale for therapeutical approachesBenoit Stijlemans
Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium
Endocr Metab Immune Disord Drug Targets 10:71-82. 2010....- Role of iron homeostasis in trypanosomiasis-associated anemiaBenoit Stijlemans
Laboratory of Cellular and Molecular Immunology, VIB Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel VUB, Building E, Level 8, Pleinlaan 2, B 1050 Brussels, Belgium
Immunobiology 213:823-35. 2008.... - A glycosylphosphatidylinositol-based treatment alleviates trypanosomiasis-associated immunopathologyBenoit Stijlemans
Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Pleinlaan 2, Brussels, Belgium
J Immunol 179:4003-14. 2007.... - African trypanosomosis: from immune escape and immunopathology to immune interventionBenoit Stijlemans
Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
Vet Parasitol 148:3-13. 2007..Finally, the possibility to interfere with such activation states hereby providing new therapeutical modalities in the treatment of this infectious disease will be illustrated... - Parallel selection of multiple anti-infectome Nanobodies without access to purified antigensDirk Saerens
Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussels, Pleinlaan 2, B 1050 Brussels, Belgium
J Immunol Methods 329:138-50. 2008..Due to the omnipresence of these targets on different parasite species and forms, these antibody fragments are a valuable source for validation of novel, not yet identified targets to design new diagnostics and therapeutics... - Identification of a parasitic immunomodulatory protein triggering the development of suppressive M1 macrophages during African trypanosomiasisJulio Gomez-Rodriguez
Department of Molecular and Cellular Interactions, Vlaams Institute voor Biotechnologie VIB, Vrije Universiteit Brussel, 1050 Brussels, Belgium
J Infect Dis 200:1849-60. 2009..TSIF knock-down trypanosomes died within 2 days, indicating that TSIF may be essential for parasite biology... - Experimental therapy of African trypanosomiasis with a nanobody-conjugated human trypanolytic factorToya Nath Baral
Department of Cellular and Molecular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Laboratorium voor Cellulaire en Moleculaire Immunologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
Nat Med 12:580-4. 2006..Treatment with this engineered conjugate resulted in clear curative and alleviating effects on acute and chronic infections of mice with both NHS-resistant and NHS-sensitive trypanosomes... - Understanding the role of monocytic cells in liver inflammation using parasite infection as a modelTom Bosschaerts
Department of Molecular and Cellular Interactions, VIB, Brussel, Belgium
Immunobiology 214:737-47. 2009.... - P75 tumor necrosis factor-receptor shedding occurs as a protective host response during African trypanosomiasisStefan Magez
Laboratory of Cellular and Molecular Immunology, Department of Cellular and Molecular Recognition, Flanders Interuniversity Institute for Biotechnology, Free University of Brussels VUB, Brussels, Belgium
J Infect Dis 189:527-39. 2004.... - Tsetse fly saliva accelerates the onset of Trypanosoma brucei infection in a mouse model associated with a reduced host inflammatory responseGuy Caljon
Unit of Cellular and Molecular Immunology, Flanders Interuniversity Institute for Biotechnology VIB, Vrije Universiteit Brussel VUB, Pleinlaan 2, B 1050 Brussels, Belgium
Infect Immun 74:6324-30. 2006..We propose that tsetse fly saliva accelerates the onset of trypanosome infection by inhibiting local and systemic inflammatory responses involved in parasite control... - Tip-DC development during parasitic infection is regulated by IL-10 and requires CCL2/CCR2, IFN-gamma and MyD88 signalingTom Bosschaerts
Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium
PLoS Pathog 6:e1001045. 2010..Therefore, investigating the contribution of recruited monocytes to African trypanosome induced liver injury could potentially identify new targets to treat hepatic inflammation in general, and during parasite infection in particular... - IL-10 limits production of pathogenic TNF by M1 myeloid cells through induction of nuclear NF-κB p50 member in Trypanosoma congolense infection-resistant C57BL/6 miceTom Bosschaerts
Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, Brussels, Belgium
Eur J Immunol 41:3270-80. 2011..congolense infection in resistant C57BL/6 mice... - Lack of galectin-3 alleviates trypanosomiasis-associated anemia of inflammationAnn Vankrunkelsven
Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Immunobiology 215:833-41. 2010..Our data indicate that Gal-3 is involved in the development of inflammation-associated anemia during African trypanosomiasis, possibly due to a disturbed iron metabolism that in turn may also lead to liver malfunction... - Efficient targeting of conserved cryptic epitopes of infectious agents by single domain antibodies. African trypanosomes as paradigmBenoit Stijlemans
Department of Cellular and Molecular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium
J Biol Chem 279:1256-61. 2004..This was illustrated by the specific trypanolysis induced by an antibody::beta-lactamase fusion activating a prodrug... - Affinity is an important determinant of the anti-trypanosome activity of nanobodiesGuy Caljon
Department of Biomedical Sciences, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium
PLoS Negl Trop Dis 6:e1902. 2012..The anti-trypanosomal activity relies on perturbing the highly active recycling of the Variant-specific Surface Glycoprotein (VSG) that occurs in the parasite's flagellar pocket... - Current status of vaccination against African trypanosomiasisStefan Magez
Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel VUB, Pleinlaan 2, B 1050 Brussels, Belgium
Parasitology 137:2017-27. 2010..This paper reviews past and current attempts to design both anti-trypanosome vaccines, as well as vaccines directed towards the inhibition of infection-associated pathology... - Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activityGuy Caljon
Department of Biomedical Sciences, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp ITM, Antwerp, Belgium
PLoS ONE 7:e47233. 2012..Knockdown of Tsal expression by in vivo RNA interference in the tsetse fly revealed a partially impaired blood digestion phenotype as evidenced by higher gut nucleic acid, hematin and protein contents... - Bacterial lipoprotein-based vaccines induce tumor necrosis factor-dependent type 1 protective immunity against Leishmania majorJavier Cote-Sierra
Department of Immunology, Parasitology and Ultrastructure, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Sint Genesius Rode, Belgium
Infect Immun 70:240-8. 2002..These data indicate that bacterial lipoproteins constitute appropriate adjuvants to include in vaccines against diseases in which type 1 immune responses are important for protection... - Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VHKatja Conrath
Laboratorium voor Cellulaire en Moleculaire Immunologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
J Mol Biol 350:112-25. 2005.... - VSG-GPI anchors of African trypanosomes: their role in macrophage activation and induction of infection-associated immunopathologyStefan Magez
Laboratory of Cellular Immunology, Free University of Brussels Flemish Interuniversity, Institute for Biotechnology, Paardenstraat 65, Sint Genesius Rode, Belgium
Microbes Infect 4:999-1006. 2002..Their interaction with various cells of the immune system underlies the severe infection-associated pathology. Recent results have shown that anti-GPI vaccination can prevent the occurrence of this pathology... - [Experimental therapy of African trypanosomiasis with a nanobody-conjugated human trypanolytic factor]Toya Nath Baral
, Vlaams Interuniversitair Instituut voor Biotechnologie, Laboratorium voor Cellulaire en Moleculaire Immunologie, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Bruxelles, Belgique
Med Sci (Paris) 22:914-6. 2006 - Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in miceHannelie Korf
Laboratory of Mycobacterial Immunology, Scientific Institute of Public Health, Brussels, Belgium
J Clin Invest 119:1626-37. 2009..The dependence of LXR signaling on the neutrophil IL-17 axis represents what we believe to be a novel function for these nuclear receptors in resistance to M. tuberculosis infection and may provide a new target for therapeutics...