Research Topics
| Jacques van HeldenSummaryCountry: Belgium Publications
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Detail Information
Publications
Machine learning techniques to identify putative genes involved in nitrogen catabolite repression in the yeast Saccharomyces cerevisiaeKevin Kontos
Machine Learning Group, , , , Boulevard du Triomphe CP 212, 1050 Brussels, Belgium
BMC Proc 2:S5. 2008..CONCLUSION : These results suggest that our approach can successfully identify potential NCR genes. Hence, the dimensionality of the problem of identifying all genes involved in NCR is drastically reduced...
Regulatory sequence analysis toolsJacques van Helden
Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, Campus Plaine, CP 263, Bld du Triomphe, B 1050 Bruxelles, Belgium
Nucleic Acids Res 31:3593-6. 2003..RSAT currently holds >100 fully sequenced genomes and these data are regularly updated from GenBank...
Unraveling networks of co-regulated genes on the sole basis of genome sequencesSylvain Brohée
Lab Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles ULB, CP 263, Campus Plaine, Bld du Triomphe, 1050 Brussels, Belgium
Nucleic Acids Res 39:6340-58. 2011..Long-term benefits can easily be perceived when considering the exponential increase of new genome sequences...
Using RSAT to scan genome sequences for transcription factor binding sites and cis-regulatory modulesJean Valery Turatsinze
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Université Libre de Bruxelles CP 263, Campus Plaine, Boulevard du Triomphe, Bruxelles, Belgium
Nat Protoc 3:1578-88. 2008..This protocol is also tested on random control sequences to evaluate the reliability of the predictions. Each task requires a few minutes of computation time on the server. The complete protocol can be executed in about one hour...
NeAT: a toolbox for the analysis of biological networks, clusters, classes and pathwaysSylvain Brohée
Laboratoire de Bioinformatique des Génomes et Réseaux BiGRE, Universite Libre de Bruxelles ULB, Boulevard du Triomphe, CP263, B 1050 Bruxelles, Belgium
Nucleic Acids Res 36:W444-51. 2008..NeAT can also be used as web services (SOAP/WSDL interface), in order to design programmatic workflows and integrate them with other available resources...
Using RSAT oligo-analysis and dyad-analysis tools to discover regulatory signals in nucleic sequencesMatthieu Defrance
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Campus Plaine, CP 263, Boulevard du Triomphe, Bruxelles, Belgium
Nat Protoc 3:1589-603. 2008..Computation times vary from seconds (gene clusters) to minutes (whole genomes). The execution of the whole protocol should take approximately 1 h...
Analyzing multiple data sets by interconnecting RSAT programs via SOAP Web services: an example with ChIP-chip dataOlivier Sand
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Campus Plaine, CP 263, Boulevard du Triomphe, Bruxelles, Belgium
Nat Protoc 3:1604-15. 2008..This protocol is addressed to bioinformaticians and biologists with programming skills (notions of Perl). Running time is approximately 6 min on the example data set...
Retrieve-ensembl-seq: user-friendly and large-scale retrieval of single or multi-genome sequences from EnsemblOlivier Sand
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Université Libre de Bruxelles CP 263, Campus Plaine, Boulevard du Triomphe, B 1050 Bruxelles, Belgium
Bioinformatics 25:2739-40. 2009..The complete manual, a convenient tutorial and demos are available from the RSAT website. Additional help can be found on the RSAT public forum...
Pathway discovery in metabolic networks by subgraph extractionKaroline Faust
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Bruxelles, Belgium
Bioinformatics 26:1211-8. 2010..Although these approaches have been adapted to metabolic networks, they are generic enough to be adjusted to other biological networks as well...
Network Analysis Tools: from biological networks to clusters and pathwaysSylvain Brohée
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Campus Plaine, CP 263, Boulevard du Triomphe, Bruxelles, Belgium
Nat Protoc 3:1616-29. 2008..e., clusters or paths) or tables displaying statistics. Typical networks comprising several thousands of nodes and arcs can be analyzed within a few minutes. The complete protocol can be read and executed in approximately 1 h...
Prophinder: a computational tool for prophage prediction in prokaryotic genomesGipsi Lima-Mendez
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, 1050 Bruxelles, Belgium
Bioinformatics 24:863-5. 2008..AVAILABILITY: Softare is available at http://aclame.ulb.ac.be/prophinder..
info-gibbs: a motif discovery algorithm that directly optimizes information content during samplingMatthieu Defrance
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Université Libre de Bruxelles CP 263, Campus Plaine, Boulevard du Triomphe, B 1050 Bruxelles, Belgium
Bioinformatics 25:2715-22. 2009..However, these information-based metrics are usually used as a posteriori statistics rather than during the motif search process itself...
RSAT: regulatory sequence analysis toolsMorgane Thomas-Chollier
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Campus Plaine, CP 263 Bld du Triomphe, B 1050 Bruxelles, Belgium
Nucleic Acids Res 36:W119-27. 2008..Since 1998, the tools have been used by several hundreds of researchers from all over the world. Several predictions made with RSAT were validated experimentally and published...
Modularity of the transcriptional response of protein complexes in yeastNicolas Simonis
, Centre de Biologie Structurale et Bioinformatique, CP 263, , Bld. du Triomphe B-1050 Bruxelles, Belgium
J Mol Biol 363:589-610. 2006..Together these findings suggest that transcriptional modules may represent basic functional and evolutionary building blocs of protein complexes...
Evaluation of clustering algorithms for protein-protein interaction networksSylvain Brohée
Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, CP 263, Campus Plaine, Bd, du Triomphe, B 1050 Bruxelles, Belgium
BMC Bioinformatics 7:488. 2006..In this paper we present a comparative assessment of four algorithms: Markov Clustering (MCL), Restricted Neighborhood Search Clustering (RNSC), Super Paramagnetic Clustering (SPC), and Molecular Complex Detection (MCODE)...
Effect of 21 different nitrogen sources on global gene expression in the yeast Saccharomyces cerevisiaePatrice Godard
Physiologie Moléculaire de la Cellule, IBMM, Universite Libre de Bruxelles, Rue des Pr Jeener et Brachet 12, 6041 Gosselies, Belgium
Mol Cell Biol 27:3065-86. 2007..Similar studies conducted for other nutrients should provide a more complete view of alternative metabolic pathways in yeast and contribute to the attribution of functions to many other orphan genes...
In silico identification of NF-kappaB-regulated genes in pancreatic beta-cellsNajib Naamane
Laboratory of Experimental Medicine, Universite Libre de Bruxelles, Route de Lennik, 808, CP 618, B 1070 Brussels, Belgium
BMC Bioinformatics 8:55. 2007..To identify NF-kappaB-regulated gene networks in beta-cells we presently used a discriminant analysis-based approach to predict NF-kappaB responding genes on the basis of putative regulatory elements...
Bacterial molecular networks: bridging the gap between functional genomics and dynamical modellingJacques van Helden
Laboratoire de Bioinformatique des Génomes et des Réseaux, Universite Libre de Bruxelles, Bruxelles, Belgium
Methods Mol Biol 804:1-11. 2012..Altogether, the chapters provide a representative overview of state-of-the-art methods for data integration and retrieval, network visualization, graph analysis, and dynamical modelling...
Metabolic PathFinding: inferring relevant pathways in biochemical networksDidier Croes
SCMBB, , Campus Plaine, CP 263, Boulevard du Triomphe, B-1050 Bruxelles, Belgium
Nucleic Acids Res 33:W326-30. 2005..Multiple requests can be submitted in a queue. Results are returned by email, in textual as well as graphical formats (available in http://www.scmbb.ulb.ac.be/pathfinding/)...
Discovery of motifs in promoters of coregulated genesOlivier Sand
Service de Conformation des Macromolécules, Biologiques et de Bioinformatiques, Universite Libre de Bruxelles, Belgium
Methods Mol Biol 395:329-48. 2007..It can be used with higher organisms as well, but with a loss in reliability of the predictions. A web interface is available at the Regulatory Sequence Analysis Tools site (http://rsat.scmbb.ulb.ac.be/rsat/)...
Reticulate representation of evolutionary and functional relationships between phage genomesGipsi Lima-Mendez
Service de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Bruxelles, Belgium
Mol Biol Evol 25:762-77. 2008..These 2 classification schemes provide an automatic and dynamic way of representing the relationships within the phage population and can be extended to include newly sequenced phage genomes, as well as other types of genetic elements...
Metabolic pathfinding using RPAIR annotationKaroline Faust
Laboratoire de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Campus Plaine, CP 263, Bld du Triomphe, B 1050 Bruxelles, Belgium
J Mol Biol 388:390-414. 2009..Our pathfinding approach is available as part of the Network Analysis Tools...
RSAT 2011: regulatory sequence analysis toolsMorgane Thomas-Chollier
Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
Nucleic Acids Res 39:W86-91. 2011..Availability (web site, stand-alone programs and SOAP/WSDL (Simple Object Access Protocol/Web Services Description Language) web services): http://rsat.ulb.ac.be/rsat/...
Evaluation of phylogenetic footprint discovery for predicting bacterial cis-regulatory elements and revealing their evolutionRekin s Janky
Laboratoire de Bioinformatique des Génomes et des Réseaux, Universite Libre de Bruxelles ULB, Campus Plaine, CP 263, Boulevard du Triomphe, 1050 Bruxelles, Belgium
BMC Bioinformatics 9:37. 2008..However, these tools are generally used as black boxes, with default parameters. A systematic evaluation of optimal parameters for a footprint discovery strategy can bring a sizeable improvement to the predictions...
Inferring meaningful pathways in weighted metabolic networksDidier Croes
, Campus Plaine, CP 263, Boulevard du Triomphe, 1050 Bruxelles, Belgium
J Mol Biol 356:222-36. 2006..We expect our approach to find useful applications in inferring metabolic pathways in newly sequenced genomes...
Metrics for comparing regulatory sequences on the basis of pattern countsJacques van Helden
SCMBB, Universite Libre de Bruxelles, Campus Plaine CP 263, Boulevard du Triomphe, B 1050 Bruxelles, Belgium
Bioinformatics 20:399-406. 2004..We compare these metrics to several classical dissimilarity and similarity metrics, and illustrate their behaviour with a biological example...
The powerful law of the power law and other myths in network biologyGipsi Lima-Mendez
Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Campus Plaine, CP 263, Boulevard du Triomphe, B 1050 Bruxelles, Belgium
Mol Biosyst 5:1482-93. 2009....
Discrimination of yeast genes involved in methionine and phosphate metabolism on the basis of upstream motifsDidier Gonze
, , CP 263, Campus Plaine, Blvd du Triomphe, B-1050 Bruxelles, Belgium
Bioinformatics 21:3490-500. 2005..scmbb.ulb.ac.be/rsat/). CONTACT: SUPPLEMENTARY INFORMATION: The complete datasets and results are available at http://rsat.scmbb.ulb.ac.be/rsat/data/published_data/Gonze_MET_PHO/..
Transcriptional regulation of protein complexes in yeastNicolas Simonis
, Centre de Biologie Structurale et Bioinformatique, CP 263, , Bld du Triomphe, B-1050 Bruxelles, Belgium
Genome Biol 5:R33. 2004....
Assessing computational tools for the discovery of transcription factor binding sitesMartin Tompa
Department of Computer Science and Engineering, Box 352350, University of Washington, Seattle, Washington 98195 2350, USA
Nat Biotechnol 23:137-44. 2005..The purpose of the current assessment is twofold: to provide some guidance to users regarding the accuracy of currently available tools in various settings, and to provide a benchmark of data sets for assessing future tools...
Fine-tuning enhancer models to predict transcriptional targets across multiple genomesStein Aerts
Laboratory of Neurogenetics, Department of Molecular and Developmental Genetics, Vlaams Instituut voor Biotechnologie VIB, Leuven, Belgium
PLoS ONE 2:e1115. 2007..The ability to model known TF-TG relations, together with the use of multiple genomes, results in a significant step forward in solving the architecture of gene regulatory networks...
Discovery of conserved motifs in promoters of orthologous genes in prokaryotesJacques van Helden
Universite Libre de Bruxelles
Methods Mol Biol 395:293-308. 2007..The method has been used successfully to detect regulatory elements at various taxonomical levels in prokaryotes. A web interface is available at the Regulatory Sequence Analysis Tools site (http://rsat.scmbb.ulb.ac.be/rsat/)...
Discovery of functional elements in 12 Drosophila genomes using evolutionary signaturesAlexander Stark
The Broad Institute, Massachusetts Institute of Technology and Harvard University, Cambridge, Massachusetts 02140, USA
Nature 450:219-32. 2007..We also study how discovery power scales with the divergence and number of species compared, and we provide general guidelines for comparative studies...
An overview of data models for the analysis of biochemical pathwaysYves Deville
Computing Science and Engineering Department, Universite Catholique de Louvain, Louvain la Neuve, Belgium
Brief Bioinform 4:246-59. 2003..This enables us to underline the strengths and weaknesses of the different approaches, as well as to highlight relevant future research directions...
Molecular evolution of eukaryotic genomes: hemiascomycetous yeast spliceosomal intronsElisabeth Bon
, Institut National Agronomique Paris-Grignon, F-78850 Thiverval-Grignon, France
Nucleic Acids Res 31:1121-35. 2003....
