H Goossens

Summary

Affiliation: University Hospital Antwerp
Country: Belgium

Publications

  1. pmc Differences in gut microbiota composition between obese and lean children: a cross-sectional study
    Liene Bervoets
    Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium
    Gut Pathog 5:10. 2013
  2. pmc General practitioners' views on the acceptability and applicability of a web-based intervention to reduce antibiotic prescribing for acute cough in multiple European countries: a qualitative study prior to a randomised trial
    Sibyl Anthierens
    Centre for General Practice, Vaccine and Infectious Disease Institute VAXINFECTIO, Laboratory of Microbiology, University of Antwerp, Antwerp, Belgium
    BMC Fam Pract 13:101. 2012
  3. pmc Nosocomial acquisition of methicillin-resistant Staphyloccocus aureus (MRSA) and extended-spectrum beta-lactamase (ESBL) Enterobacteriaceae in hospitalised patients: a prospective multicenter study
    Giulia De Angelis
    Department of Infectious Diseases, Universita Cattolica del Sacro Cuore, Largo F, VITO, 1 00168 Rome, Italy
    BMC Infect Dis 12:74. 2012
  4. pmc Short-term cultured, interleukin-15 differentiated dendritic cells have potent immunostimulatory properties
    Sébastien Anguille
    University of Antwerp Faculty of Medicine, Vaccine and Infectious Disease Institute VAXINFECTIO, Laboratory of Experimental Hematology, Universiteitsplein 1, B 2610 Wilrijk Antwerp, Belgium
    J Transl Med 7:109. 2009
  5. pmc Intestinal microflora and body mass index during the first three years of life: an observational study
    Carl Vael
    Department of Microbiology, University of Antwerp, Antwerp, Belgium
    Gut Pathog 3:8. 2011
  6. pmc Early intestinal Bacteroides fragilis colonisation and development of asthma
    Carl Vael
    Department of Microbiology, University of Antwerp, Antwerp, Belgium
    BMC Pulm Med 8:19. 2008
  7. pmc The appropriateness of prescribing antibiotics in the community in Europe: study design
    Evelien M E van Bijnen
    NIVEL, Netherlands Institute for Health Services Research, 3500 BN Utrecht, The Netherlands
    BMC Infect Dis 11:293. 2011
  8. ncbi request reprint European status of resistance in nosocomial infections
    Herman Goossens
    University Hospital Antwerp, Antwerp, Belgium
    Chemotherapy 51:177-81. 2005
  9. ncbi request reprint Prevalence and antimicrobial susceptibility data for extended-spectrum beta-lactamase- and AmpC-producing Enterobacteriaceae from the MYSTIC Program in Europe and the United States (1997-2004)
    Herman Goossens
    Department of Microbiology, University Hospital Antwerp, Edegem Antwerp, B 2650, Belgium
    Diagn Microbiol Infect Dis 53:257-64. 2005
  10. ncbi request reprint Comparison of outpatient systemic antibacterial use in 2004 in the United States and 27 European countries
    Herman Goossens
    University of Antwerp, Antwerp, Belgium
    Clin Infect Dis 44:1091-5. 2007

Detail Information

Publications108 found, 100 shown here

  1. pmc Differences in gut microbiota composition between obese and lean children: a cross-sectional study
    Liene Bervoets
    Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium
    Gut Pathog 5:10. 2013
    ..Secondly, associations between analysed gut bacterial species, dietary compounds, energy intake and biochemical blood parameters are evaluated...
  2. pmc General practitioners' views on the acceptability and applicability of a web-based intervention to reduce antibiotic prescribing for acute cough in multiple European countries: a qualitative study prior to a randomised trial
    Sibyl Anthierens
    Centre for General Practice, Vaccine and Infectious Disease Institute VAXINFECTIO, Laboratory of Microbiology, University of Antwerp, Antwerp, Belgium
    BMC Fam Pract 13:101. 2012
    ..We explored GPs' views on the initial version of INTRO to test acceptability and potentially increase applicability for use in multiple countries before the start of a randomised trial...
  3. pmc Nosocomial acquisition of methicillin-resistant Staphyloccocus aureus (MRSA) and extended-spectrum beta-lactamase (ESBL) Enterobacteriaceae in hospitalised patients: a prospective multicenter study
    Giulia De Angelis
    Department of Infectious Diseases, Universita Cattolica del Sacro Cuore, Largo F, VITO, 1 00168 Rome, Italy
    BMC Infect Dis 12:74. 2012
    ....
  4. pmc Short-term cultured, interleukin-15 differentiated dendritic cells have potent immunostimulatory properties
    Sébastien Anguille
    University of Antwerp Faculty of Medicine, Vaccine and Infectious Disease Institute VAXINFECTIO, Laboratory of Experimental Hematology, Universiteitsplein 1, B 2610 Wilrijk Antwerp, Belgium
    J Transl Med 7:109. 2009
    ..The applicability of these so-called IL-15 DCs has not yet been firmly established. We therefore developed a novel pre-clinical approach for the generation of IL-15 DCs with potent immunostimulatory properties...
  5. pmc Intestinal microflora and body mass index during the first three years of life: an observational study
    Carl Vael
    Department of Microbiology, University of Antwerp, Antwerp, Belgium
    Gut Pathog 3:8. 2011
    ..abstract:..
  6. pmc Early intestinal Bacteroides fragilis colonisation and development of asthma
    Carl Vael
    Department of Microbiology, University of Antwerp, Antwerp, Belgium
    BMC Pulm Med 8:19. 2008
    ..The aim of the study was to investigate the association between early intestinal colonisation and the development of asthma in the first 3 years of life...
  7. pmc The appropriateness of prescribing antibiotics in the community in Europe: study design
    Evelien M E van Bijnen
    NIVEL, Netherlands Institute for Health Services Research, 3500 BN Utrecht, The Netherlands
    BMC Infect Dis 11:293. 2011
    ..We will also evaluate the appropriateness of national antibiotic prescription guidelines in relation to resistance patterns...
  8. ncbi request reprint European status of resistance in nosocomial infections
    Herman Goossens
    University Hospital Antwerp, Antwerp, Belgium
    Chemotherapy 51:177-81. 2005
    ..Surveillance studies are mandatory to carefully monitor changing trends in Europe...
  9. ncbi request reprint Prevalence and antimicrobial susceptibility data for extended-spectrum beta-lactamase- and AmpC-producing Enterobacteriaceae from the MYSTIC Program in Europe and the United States (1997-2004)
    Herman Goossens
    Department of Microbiology, University Hospital Antwerp, Edegem Antwerp, B 2650, Belgium
    Diagn Microbiol Infect Dis 53:257-64. 2005
    ..0% for both). The continued efficacy of carbapenems such as meropenem confirms that these remain first-line agents for treatment of nosocomial infections caused by Enterobacteriaceae-producing ESBL or AmpC beta-lactamases...
  10. ncbi request reprint Comparison of outpatient systemic antibacterial use in 2004 in the United States and 27 European countries
    Herman Goossens
    University of Antwerp, Antwerp, Belgium
    Clin Infect Dis 44:1091-5. 2007
    ....
  11. ncbi request reprint Outpatient antibiotic use in Europe and association with resistance: a cross-national database study
    Herman Goossens
    ESAC Management Team, Department of Microbiology, University of Antwerp, B 2610 Antwerp, Belgium
    Lancet 365:579-87. 2005
    ..Our aim was to assess outpatient use of antibiotics and the association with resistance...
  12. ncbi request reprint National campaigns to improve antibiotic use
    Herman Goossens
    Laboratory of Medical Microbiology, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium
    Eur J Clin Pharmacol 62:373-9. 2006
    ..The benefits and risks of such campaigns have been less well established in countries where antibiotic use is already low or declining...
  13. ncbi request reprint Results of two worldwide surveys into physician awareness and perceptions of extended-spectrum beta-lactamases
    H Goossens
    Department of Microbiology, University Hospital, Antwerp, Belgium
    Clin Microbiol Infect 10:760-2. 2004
    ..The surveys demonstrated that awareness of, and testing for, ESBLs is inconsistent...
  14. ncbi request reprint European survey of vancomycin-resistant enterococci in at-risk hospital wards and in vitro susceptibility testing of ramoplanin against these isolates
    Herman Goossens
    University Hospital Antwerp and Belgian Reference Centre of Enterococci, Edegem, Belgium
    J Antimicrob Chemother 51:iii5-12. 2003
    ..This survey suggests that in some centres in Europe, a similar situation may be encountered to that in the USA (monoclonal spread of multi-resistant VRE in at-risk wards)...
  15. ncbi request reprint Susceptibility of multi-drug-resistant Pseudomonas aeruginosa in intensive care units: results from the European MYSTIC study group
    H Goossens
    Department of Microbiology, University Hospital, Edegem Antwerp, Belgium
    Clin Microbiol Infect 9:980-3. 2003
    ..7% (32/>128), meropenem 29.1% (8/64), imipenem 44.9% (16/64), cefepime 49.5% (32/64) and piperacillin/tazobactam 57.9% (128/>128)...
  16. doi request reprint Antibiotic consumption and link to resistance
    H Goossens
    Department of Medical Microbiology, University Hospital Antwerp, Antwerp, Belgium
    Clin Microbiol Infect 15:12-5. 2009
    ..Higher consumption of clarithromycin in Europe correlated with a predominance of erm(B)-carrying Streptococcus pneumoniae. The erm(B) gene caused resistance in 84% of the isolates in Europe...
  17. pmc Development of real-time multiplex nucleic acid sequence-based amplification for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. in respiratory specimens
    K Loens
    Department of Medical Microbiology, University of Antwerp, Universiteitsplein 1 S009a, B 2610 Wilrijk, Belgium
    J Clin Microbiol 46:185-91. 2008
    ..pneumoniae, C. pneumoniae, and Legionella spp. in respiratory specimens, regarding handling, speed, and number of samples that can be analyzed in a single run...
  18. pmc Molecular epidemiology of recent belgian isolates of Neisseria meningitidis serogroup B
    M Van Looveren
    Department of Microbiology, University Hospital Antwerp, UIA, Antwerp, Belgium
    J Clin Microbiol 36:2828-34. 1998
    ..4 and B:nontypeable:P1.4, but strains with several other phenotypes were also encountered. Therefore, serotyping alone would underestimate the prevalence of the epidemic clone...
  19. doi request reprint Evaluation of different nucleic acid amplification techniques for the detection of M. pneumoniae, C. pneumoniae and Legionella spp. in respiratory specimens from patients with community-acquired pneumonia
    K Loens
    Department of Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp UIA, Antwerp, Belgium
    J Microbiol Methods 73:257-62. 2008
    ..It's practical attractiveness pleads for further optimalisation of the multiplex approach...
  20. ncbi request reprint Evaluation of the NucliSens miniMAG RNA extraction and real-time NASBA applications for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in throat swabs
    K Loens
    Department of Microbiology, University of Antwerp UA, Universiteitsplein 1 S009a, Antwerp Wilrijk, Belgium
    J Microbiol Methods 72:217-9. 2008
    ..The NucliSens miniMAG and NucliSens EasyQ applications were successfully coupled to detect both organisms in throat swabs...
  21. pmc Development of conventional and real-time nucleic acid sequence-based amplification assays for detection of Chlamydophila pneumoniae in respiratory specimens
    K Loens
    Department of Medical Microbiology, University of Antwerp, Universiteitsplein 1 S3, B 2610 Wilrijk, Belgium
    J Clin Microbiol 44:1241-4. 2006
    ..1 and 1 IFU/100 mul sample, depending on the type of specimen. Finally, conventional and real-time NASBA were applied to respiratory specimens previously tested by PCR. A 100% concordance between the test results was obtained...
  22. ncbi request reprint Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human medicine
    P Descheemaeker
    Department of Microbiology, University Hospital Antwerp, Universitaire Instelling Antwerpen, Belgium
    Int J Syst Bacteriol 47:555-61. 1997
    ..flavescens. PFGE did not result in species-discriminative DNA bands or band patterns, but proved to be superior for interpretation of interstrain relationships...
  23. pmc Comparison of glycopeptide-resistant Enterococcus faecium isolates and glycopeptide resistance genes of human and animal origins
    P R Descheemaeker
    Belgian Reference Centre for Enterococci, Department of Microbiology, University Hospital Antwerp, Universitaire Instelling Antwerpen, Antwerp, University of Ghent, Ghent, Belgium
    Antimicrob Agents Chemother 43:2032-7. 1999
    ..Type 2 transposons were significantly associated with pig isolates. The other types were seldom detected. These data suggest a possible exchange of glycopeptide resistance markers between animals and humans...
  24. ncbi request reprint High-dose cefepime as an alternative treatment for infections caused by TEM-24 ESBL-producing Enterobacter aerogenes in severely-ill patients
    K Goethaert
    Department of Microbiology, University Hospital of Antwerp, Edegem, Belgium
    Clin Microbiol Infect 12:56-62. 2006
    ..It was concluded that cefepime may be an alternative agent for therapy of severe infections caused by TEM-24 ESBL-producing E. aerogenes, although further studies are required to confirm these observations...
  25. ncbi request reprint Culture-based detection of methicillin-resistant Staphylococcus aureus by a network of European laboratories: an external quality assessment study
    M Gazin
    Department of Medical Microbiology, Vaccine and Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium
    Eur J Clin Microbiol Infect Dis 31:1765-70. 2012
    ....
  26. doi request reprint Evaluation of GeneOhm VanR and Xpert vanA/vanB molecular assays for the rapid detection of vancomycin-resistant enterococci
    M Gazin
    National Reference Centre for Enterococcus spp, Department of Medical Microbiology, Vaccine and Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium
    Eur J Clin Microbiol Infect Dis 31:273-6. 2012
    ..87.5%), but equally non-specific (20.6% vs. 14.7%). A further estimation on log-serial dilutions of VRE strains showed that Xpert was more sensitive at detecting VRE at low concentrations (10-100 colony forming units [cfu]/ml)...
  27. pmc Evaluation of NucliSens easyMAG for automated nucleic acid extraction from various clinical specimens
    K Loens
    Department of Medical Microbiology, University of Antwerp, Universiteitsplein 1 S009a, B 2610 Wilrijk, Belgium
    J Clin Microbiol 45:421-5. 2007
    ..95 (range, -5.51 to +1.68). The NucliSens easyMAG is considerably easier to perform, efficiently extracts nucleic acids from throat swabs and whole blood, is automated, and has high throughput...
  28. ncbi request reprint Outbreak of hospital-adapted clonal complex-17 vancomycin-resistant Enterococcus faecium strain in a haematology unit: role of rapid typing for early control
    A Deplano
    Department of Microbiology, Erasme Hospital, Universite Libre de Bruxelles, Brussels, Belgium
    J Antimicrob Chemother 60:849-54. 2007
    ..To describe the investigation and molecular characterization of a vancomycin-resistant Enterococcus faecium (VREF) strain responsible for a nosocomial outbreak in the haematology unit of a tertiary-care university hospital...
  29. doi request reprint A multicenter pilot external quality assessment programme to assess the quality of molecular detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae
    K Loens
    Department of Microbiology, University of Antwerp, Antwerp, Belgium
    J Microbiol Methods 82:131-5. 2010
    ..The percentage of correct qualitative results for M. pneumoniae demonstrated that the concentrations included in this panel proved challenging for a number of participants...
  30. ncbi request reprint Hospital consumption of antibiotics in 15 European countries: results of the ESAC Retrospective Data Collection (1997-2002)
    R H Vander Stichele
    ESAC Management Team, Laboratory of Medical Microbiology, University of Antwerp, Universiteitsplein 1 B 2610 Antwerp, Belgium
    J Antimicrob Chemother 58:159-67. 2006
    ..To collect reliable, comparable and publicly available data on hospital use of antibiotics in Europe aggregated at the national level (1997-2002)...
  31. ncbi request reprint MYSTIC (Meropenem Yearly Susceptibility Test Information Collection) results from Europe: comparison of antibiotic susceptibilities between countries and centre types. MYSTIC Study Group (European centres only)
    H Goossens
    Department of Microbiology, University Hospital, Edegem Antwerp, Belgium
    J Antimicrob Chemother 46:39-52. 2000
    ..No increase in resistance to the carbapenems, to date, has been detected in any of the European centres included in this study...
  32. pmc Survey of enterococcal susceptibility patterns in Belgium
    P Vandamme
    Department of Microbiology, University Hospital UIA, Antwerp, Belgium
    J Clin Microbiol 34:2572-6. 1996
    ..E. faecium is generally more resistant to a wide range of antibiotics, but glycopeptide-resistant strains (1.5%) have not yet become widespread...
  33. doi request reprint Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods
    K Loens
    Department of Microbiology, Vaccine and Infectious Disease Institute VIDI, University of Antwerp, Antwerp, Belgium
    Eur J Clin Microbiol Infect Dis 29:1055-69. 2010
    ..pneumoniae as a cause of respiratory tract infections (RTIs) differs from study to study due to geographical and epidemiological differences, as well as to the application of different diagnostic techniques and criteria used...
  34. pmc Evaluation of the discriminatory power of typing methods for Neisseria gonorrhoeae
    M Van Looveren
    Department of Medical Microbiology, University Hospital Antwerp, University of Antwerp, Antwerp, Belgium
    J Clin Microbiol 37:2183-8. 1999
    ..743 alone but a DI of 0.955 in combination with serotyping. PFGE using the restriction enzyme BglII and opa typing produced the highest discrimination (DIs, 0.997 and 0. 996, respectively) for isolates of N. gonorrhoeae...
  35. pmc Performance of different mono- and multiplex nucleic acid amplification tests on a multipathogen external quality assessment panel
    K Loens
    Department of Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium
    J Clin Microbiol 50:977-87. 2012
    ..However, large differences in amplification efficacy were found between the commercially available tests; acceptable results were obtained by using the PathoFinder assays...
  36. ncbi request reprint Identification of clinically relevant viridans streptococci by analysis of transfer DNA intergenic spacer length polymorphism
    Y De Gheldre
    Department of Microbiology, Hopital Erasme, Universite Libre de Bruxelles, Belgium
    Int J Syst Bacteriol 49:1591-8. 1999
    ..mitis biovar 2 strains belong to several streptococcal taxa. In conclusion, PCR analysis of tDNA-ILP holds promise for rapid identification of viridans streptococci that are difficult to identify by phenotypic tests...
  37. ncbi request reprint Antimicrobial susceptibility of nontyphoidal Salmonella isolated from humans in Belgium
    M Van Looveren
    Department of Medical Microbiology, University Hospital Antwerp, UIA, Antwerp, Belgium
    Acta Clin Belg 56:180-6. 2001
    ..25 microgram/ml) and MIC90 (1 microgram/ml) was observed for ciprofloxacin. The high rate of resistance to nalidixic acid can be a first step towards the development of resistance to ciprofloxacin...
  38. ncbi request reprint Development of conventional and real-time NASBA for the detection of Legionella species in respiratory specimens
    K Loens
    Department of Microbiology, University of Antwerp UA, Antwerp, Belgium
    J Microbiol Methods 67:408-15. 2006
    ..All 11 PCR positive samples were positive by conventional NASBA, 9/11 and 10/11 were positive by L. pneumophila real-time NASBA and Legionella spp. real-time NASBA, respectively...
  39. pmc Two quality control exercises involving nucleic acid amplification methods for detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and carried out 2 years apart (in 2002 and 2004)
    K Loens
    Laboratory of Medical Microbiology, Department of Medicine, Universitaire Instelling Antwerpen, Wilrijk, Belgium
    J Clin Microbiol 44:899-908. 2006
    ..Both multiplex PCR and nucleic acid sequence-based amplification (NASBA) formats scored a smaller number of samples positive than the monoplex reactions...
  40. ncbi request reprint MYSTIC program: summary of European data from 1997 to 2000
    H Goossens
    Department of Microbiology, University Hospital, Antwerp, Belgium
    Diagn Microbiol Infect Dis 41:183-9. 2001
    ..There was no significant decrease in susceptibility to the carbapenems throughout the four-year period. Meropenem and imipenem appear to remain reliable options for the treatment of serious nosocomial infections...
  41. pmc Comparison of direct plating and broth enrichment culture for the detection of intestinal colonization by glycopeptide-resistant enterococci among hospitalized patients
    M Ieven
    Laboratory for Microbiology, University Hospital Antwerp, B 2650 Edegem, Belgium
    J Clin Microbiol 37:1436-40. 1999
    ..The majority of isolates were polyclonal. Our data indicate that the rate of detection of GRE from both stool samples and rectal swabs is significantly increased with enrichment cultures...
  42. pmc Development of a multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium
    Vanessa Vankerckhoven
    Laboratory of Medical Microbiology, University of Antwerp, Antwerp, Belgium
    J Clin Microbiol 42:4473-9. 2004
    ..Molecular analysis showed the intrahospital spread of esp-positive VREF clones (in Italy) and hyl-positive VREF clones (in the United Kingdom); the role of hyl remains to be elucidated...
  43. ncbi request reprint Interpreting the rising incidence of meningococcal disease in Belgium: the contribution of molecular typing
    M Van Looveren
    Department of Medical Microbiology, University Hospital Antwerp, UIA, Belgium
    J Med Microbiol 50:986-90. 2001
    ..In conclusion, the ongoing increase of meningococcal disease in Belgium could be attributed not only to the further expansion of lineage III, but also to the introduction of C:2b:P1.5,2 strains of cluster A4 in to the Belgian population...
  44. doi request reprint Validation of the MicroScan-96 for the species identification and methicillin susceptibility testing of clinical significant coagulase-negative staphylococci
    L Patteet
    Laboratory of Medical Microbiology, University Hospital Antwerp, Wilrijkstraat 10, Edegem, Belgium
    Eur J Clin Microbiol Infect Dis 31:747-51. 2012
    ..The automated system can be considered a simple and reliable method for identification and methicillin susceptibility of CNS...
  45. ncbi request reprint Antimicrobial resistance of Acinetobacter spp. in Europe
    M Van Looveren
    Department of Medical Microbiology, University Hospital Antwerp, UA, Antwerp, Belgium
    Clin Microbiol Infect 10:684-704. 2004
    ..in Europe, as well as the main mechanisms of antimicrobial resistance, and summarises the remaining treatment options for multiresistant Acinetobacter infections...
  46. ncbi request reprint Molecular characterisation of group A streptococci from invasive and non-invasive disease episodes in Belgium during 1993-1994
    P Descheemaeker
    Department of Microbiology, University Hospital Antwerp, Universitaire Instelling Antwerpen, Belgium
    J Med Microbiol 49:467-71. 2000
    ..Other streptococcal virulence factors in conjunction with host factors may determine the outcome of invasive GAS infection...
  47. ncbi request reprint Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium
    M Van Looveren
    Department of Medical Microbiology, University Hospital Antwerp, UIA, Antwerp, Belgium
    J Antimicrob Chemother 48:235-40. 2001
    ..2%). Alarmingly high rates of resistance to ciprofloxacin were also noted, particularly for C. coli from broilers (62.1%). The latter indicates that resistance of Campylobacter in humans could derive from animals...
  48. ncbi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient antibiotic use in Europe, 1998-2005
    A Muller
    Vaccine and Infectious Disease Institute VIDI, University of Antwerp, Belgium
    Euro Surveill 12:E071011.1. 2007
  49. ncbi request reprint Organization of the ribosomal operon 165-235 gene spacer region in representatives of Neisseria gonorrhoeae
    M Van Looveren
    Department of Medical Microbiology, University Hospital Antwerp, University of Antwerp, Belgium
    Syst Appl Microbiol 23:9-14. 2000
    ..We demonstrated that this results from sequence divergence in the 4 rRNA operons, present in the genome of N. gonorrhoeae, giving rise to patterns that are a combination of several other patterns...
  50. pmc European Surveillance of Antimicrobial Consumption (ESAC): quality indicators for outpatient antibiotic use in Europe
    Samuel Coenen
    University of Antwerp Campus Drie Eiken, Department of General Practice, Universiteitsplein 1, 2610 Antwerp, Belgium
    Qual Saf Health Care 16:440-5. 2007
    ....
  51. doi request reprint Unusual resistance patterns in macrolide-resistant Streptococcus pyogenes harbouring erm(A)
    Surbhi Malhotra-Kumar
    Department of Medical Microbiology, Vaccine and Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium
    J Antimicrob Chemother 63:42-6. 2009
    ..Here we analysed the genetic basis of these novel phenotypes...
  52. pmc Genotypic diversity, antimicrobial resistance, and virulence factors of human isolates and probiotic cultures constituting two intraspecific groups of Enterococcus faecium isolates
    Vanessa Vankerckhoven
    Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium
    Appl Environ Microbiol 74:4247-55. 2008
    ..In combination with antimicrobial resistance and virulence testing, this grouping might represent an additional criterion in assessing the safety of new potential probiotic E. faecium isolates...
  53. pmc Detection of rhinoviruses by tissue culture and two independent amplification techniques, nucleic acid sequence-based amplification and reverse transcription-PCR, in children with acute respiratory infections during a winter season
    K Loens
    Department of Medical Microbiology, University of Antwerp, Wilrijk, Belgium
    J Clin Microbiol 44:166-71. 2006
    ..5% and 82.9, 93.4, 55.7, and 98.2%, respectively. NASBA and RT-PCR produced comparable results and were significantly more sensitive than virus culture. RVs showed the highest incidence in acute respiratory tract infections in children...
  54. pmc Impact of a short period of pre-enrichment on detection and bacterial loads of methicillin-resistant Staphylococcus aureus from screening specimens
    L Van Heirstraeten
    University of Antwerp, Antwerp, Belgium
    J Clin Microbiol 47:3326-8. 2009
    ..005)...
  55. ncbi request reprint Herpes simplex virus in the respiratory tract of critical care patients: a prospective study
    Peggy Bruynseels
    Department of Microbiology, University Hospital of Antwerp, Antwerp, Belgium
    Lancet 362:1536-41. 2003
    ..We did a prospective cohort study to define the prevalence, origin, risk factors, and clinical relevance of HSV in the respiratory tract of patients undergoing critical care...
  56. doi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient parenteral antibiotic treatment in Europe
    Samuel Coenen
    Department of General Practice, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium
    J Antimicrob Chemother 64:200-5. 2009
    ..To assess the proportion of parenteral treatment of the total outpatient antibiotic use in Europe, and to identify the antibiotic groups and individual antibiotics most commonly administered in this way...
  57. ncbi request reprint Sensitivity of detection of rhinoviruses in spiked clinical samples by nucleic acid sequence-based amplification in the presence of an internal control
    Katherine Loens
    Microbiology Centre for Molecular Diagnostics, University Hospital Antwerp, Wilrijkstraat 10, B 2650 Edegem, Belgium
    J Microbiol Methods 66:73-8. 2006
    ..NASBA allows a sensitive detection of RV RNA in spiked respiratory specimens in the presence of an internal control...
  58. ncbi request reprint Oropharyngeal carriage of macrolide-resistant viridans group streptococci: a prevalence study among healthy adults in Belgium
    Surbhi Malhotra-Kumar
    Department of Medical Microbiology, University of Antwerp, S3, Universiteitsplein 1, B 2610 Wilrijk, Antwerp, Belgium
    J Antimicrob Chemother 53:271-6. 2004
    ..Our aim was to investigate healthy adults for oropharyngeal carriage of VGS that are resistant to macrolides, as well as to other common antibiotics...
  59. pmc Induction of complete and molecular remissions in acute myeloid leukemia by Wilms' tumor 1 antigen-targeted dendritic cell vaccination
    Viggo F Van Tendeloo
    University of Antwerp, Faculty of Medicine, Vaccine and Infectious Disease Institute Vaxinfectio and Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Antwerp B 2650, Belgium
    Proc Natl Acad Sci U S A 107:13824-9. 2010
    ..These data support the further development of vaccination with WT1 mRNA-loaded dendritic cells as a postremission treatment to prevent full relapse in AML patients...
  60. ncbi request reprint Detection of Mycoplasma pneumoniae in respiratory samples by real-time PCR using an inhibition control
    Dominique Ursi
    Laboratory for Microbiology, University Hospital Antwerp, Wilrijkstraat 10, B 2650 Edegem, Antwerp, Belgium
    J Microbiol Methods 55:149-53. 2003
    ..There was 100% agreement between the assays, but the real-time PCR proved to be highly superior in speed with a much lower risk of false positives by laboratory contamination...
  61. ncbi request reprint Genetic identification of Candida species in HIV-positive patients using the polymerase chain reaction and restriction fragment length polymorphism analysis of its DNA
    J Irobi
    Department of Biochemistry, University of Antwerp, UIA, Antwerp, Belgium
    Mol Cell Probes 13:401-6. 1999
    ..tropicalis, C. dubliniensis and C. krusei could be identified following restriction digestion of the PCR products. The methods proved to be both simple and reproducible and may offer potential advantages over phenotyping methods...
  62. ncbi request reprint In vitro activity of gemifloxacin compared with other antimicrobial agents against recent clinical isolates of streptococci
    M Ieven
    Laboratory for Microbiology, University Hospital Antwerp, Wilrijkstraat 10, B 2650 Edegem, Belgium
    J Antimicrob Chemother 45:51-3. 2000
    ..pyogenes (MIC = 0.03- 0.06 mg/L) with reduced susceptibility to ofloxacin (MIC = 4-8 mg/L) and grepafloxacin (MIC = 4 mg/L). These preliminary observations indicate that gemifloxacin is a promising antimicrobial agent for clinical use...
  63. pmc Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection
    G Van Den Bosch
    Laboratory of Experimental Hematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium
    J Clin Microbiol 39:2308-10. 2001
    ..Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease...
  64. ncbi request reprint Antibiotic use in ambulatory care in Europe (ESAC data 1997-2002): trends, regional differences and seasonal fluctuations
    Monique M Elseviers
    Faculty of Medicine, Division of Nursing and Midwivery, University of Antwerp, Antwerp, Belgium
    Pharmacoepidemiol Drug Saf 16:115-23. 2007
    ..This paper reports on the retrospective data collection in ambulatory care for the period 1997-2002...
  65. pmc Bacitracin-resistant clone of Streptococcus pyogenes isolated from pharyngitis patients in Belgium
    Surbhi Malhotra-Kumar
    Department of Microbiology, University of Antwerp UIA, Antwerp, Belgium
    J Clin Microbiol 41:5282-4. 2003
    ..The emergence of a bacitracin-resistant S. pyogenes clone raises questions about the continued reliability of bacitracin susceptibility testing for S. pyogenes identification...
  66. pmc Macrolide- and telithromycin-resistant Streptococcus pyogenes, Belgium, 1999-2003
    Surbhi Malhotra-Kumar
    University of Antwerp, Antwerp, Belgium
    Emerg Infect Dis 11:939-42. 2005
    ..We also report one of the largest collections of telithromycin-resistant isolates...
  67. doi request reprint Determinants of between-country differences in ambulatory antibiotic use and antibiotic resistance in Europe: a longitudinal observational study
    A Blommaert
    Centre for Health Economic Research and Modelling Infectious Diseases CHERMID, Vaccine and Infectious Disease Institute VAXINFECTIO, University of Antwerp, Antwerp, Belgium
    J Antimicrob Chemother 69:535-47. 2014
    ..To identify key determinants explaining country-year variations in antibiotic use and resistance...
  68. pmc European surveillance of antimicrobial consumption (ESAC): data collection performance and methodological approach
    R H Vander Stichele
    ESAC Management Team, Department of Microbiology, University of Antwerp, Antwerp, Belgium
    Br J Clin Pharmacol 58:419-28. 2004
    ..Europe is a continent with strong public healthcare systems, but diverging antibiotic policies and resistance patterns...
  69. ncbi request reprint Diphtheria immunity in Flanders
    C Mathei
    Department of Epidemiology and Community Medicine, University of Antwerp, Belgium
    Eur J Clin Microbiol Infect Dis 16:631-6. 1997
    ..9% and 55.5%, respectively). These results emphasise the need for booster immunization of adults...
  70. pmc Current trends in rapid diagnostics for methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococcus species
    Surbhi Malhotra-Kumar
    Department of Medical Microbiology, Campus Drie Eiken, University of Antwerp, S3, Universiteitsplein 1, B 2610 Wilrijk, Belgium
    J Clin Microbiol 46:1577-87. 2008
  71. doi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient systemic antimycotic and antifungal use in Europe
    Niels Adriaenssens
    Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium
    J Antimicrob Chemother 65:769-74. 2010
    ..To assess the total outpatient systemic antimycotic and antifungal use in Europe, and to identify the antimycotic and antifungal substances most commonly used...
  72. doi request reprint Emergence of high-level fluoroquinolone resistance in emm6 Streptococcus pyogenes and in vitro resistance selection with ciprofloxacin, levofloxacin and moxifloxacin
    Surbhi Malhotra-Kumar
    Department of Medical Microbiology, Vaccine and Infectious Disease Institute, Universiteit Antwerpen, Belgium
    J Antimicrob Chemother 63:886-94. 2009
    ..To investigate the prevalence of fluoroquinolone resistance in Streptococcus pyogenes and its in vitro selection by ciprofloxacin and the respiratory fluoroquinolones, levofloxacin and moxifloxacin...
  73. ncbi request reprint Effect of azithromycin and clarithromycin therapy on pharyngeal carriage of macrolide-resistant streptococci in healthy volunteers: a randomised, double-blind, placebo-controlled study
    Surbhi Malhotra-Kumar
    Department of Medical Microbiology, University of Antwerp, Belgium
    Lancet 369:482-90. 2007
    ..We used the macrolides azithromycin and clarithromycin to investigate the direct effect of antibiotic exposure on resistance in the oral streptococcal flora of healthy volunteers...
  74. doi request reprint Evaluation of the ESPLINE® Influenza A & B-N assay for the detection of influenza A and B in nasopharyngeal aspirates
    E De Witte
    Laboratory of Microbiology, University Hospital Antwerp, Wilrijkstraat 10, 2650, Edegem, Antwerp, Belgium
    Eur J Clin Microbiol Infect Dis 31:761-6. 2012
    ..The test seems less sensitive to detect H1N1 compared to seasonal influenza, although the difference is borderline not significant (p = 0.067)...
  75. pmc Chlamydial infection: an accurate model for opportunistic screening in general practice
    V Verhoeven
    University of Antwerp, Department of General Practice Universiteitsplein 1 Wilrijk, 2610, Belgium
    Sex Transm Infect 79:313-7. 2003
    ..To estimate the prevalence of Chlamydia trachomatis in women in general practice and to assess risk factors associated with infection...
  76. doi request reprint Efficient mRNA electroporation of peripheral blood mononuclear cells to detect memory T cell responses for immunomonitoring purposes
    K Van Camp
    Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, Faculty of Medicine, University of Antwerp, Antwerp, Belgium
    J Immunol Methods 354:1-10. 2010
    ..This novel method is a rapid and elegant tool and will be convenient for monitoring the cellular immune status of patients in a clinical vaccination setting...
  77. pmc Detection of Mycoplasma pneumoniae by real-time nucleic acid sequence-based amplification
    K Loens
    Department of Medical Microbiology, University of Antwerp UIA, Antwerp, Belgium
    J Clin Microbiol 41:4448-50. 2003
    ..pneumoniae by RT-NASBA in respiratory samples might be slightly reduced compared to that by conventional NASBA. However, the real-time assay is superior in speed and ease of handling...
  78. pmc Multiplex PCR for simultaneous detection of macrolide and tetracycline resistance determinants in streptococci
    Surbhi Malhotra-Kumar
    Belgian Reference Center for Group A Streptococcus, University of Antwerp, Belgium
    Antimicrob Agents Chemother 49:4798-800. 2005
    ..We developed a multiplex PCR to facilitate simultaneous and specific detection of resistance determinants for both macrolides [erm(A), erm(B), and mef(A/E)] and tetracyclines [tet(M), tet(O), tet(K), and tet(L)] in streptococci...
  79. ncbi request reprint Clonal spread of fluoroquinolone non-susceptible Streptococcus pyogenes
    Surbhi Malhotra-Kumar
    Belgian Reference Centre for Group A Streptococcus, University of Antwerp, Campus Drie Eiken, S3, Universiteitsplein 1, B 2610 Wilrijk, Antwerp, Belgium
    J Antimicrob Chemother 55:320-5. 2005
    ..So far, resistance to fluoroquinolones in Streptococcus pyogenes is very rare...
  80. doi request reprint A longitudinal analysis on the association between antibiotic use, intestinal microflora, and wheezing during the first year of life
    Stijn L Verhulst
    University of Antwerp, Department of Pediatrics, Wilrijk, Belgium
    J Asthma 45:828-32. 2008
    ..To examine the association between the intestinal flora at the age of three weeks and wheezing during the first year of life in a prospective birth cohort study...
  81. ncbi request reprint Comparison of three Legionella urinary antigen assays during an outbreak of legionellosis in Belgium
    Kristien Dirven
    Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium
    J Med Microbiol 54:1213-6. 2005
    ....
  82. doi request reprint Antibiotic consumption in southern and eastern Mediterranean hospitals: results from the ARMed project
    Michael A Borg
    Infection Control Unit, Mater Dei Hospital, Msida, Malta
    J Antimicrob Chemother 62:830-6. 2008
    ..Hospitals are therefore being encouraged to undertake surveillance and benchmarking of antimicrobial consumption patterns with a view to identify and rectify possible evidence of overuse or misuse...
  83. ncbi request reprint Bacteraemia in Europe--antimicrobial susceptibility data from the MYSTIC surveillance programme
    Serhat Unal
    Department of Internal Medicine, Medical Faculty, Hacettepe University, Ankara 06100, Turkey
    Int J Antimicrob Agents 23:155-63. 2004
    ..aeruginosa and showed the highest potency and activity against all extended-spectrum and AmpC beta-lactamase producers. These results are relevant to the choice of initial, empirical therapy for patients with suspected bacteraemia...
  84. ncbi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient cephalosporin use in Europe
    Samuel Coenen
    Department of General Practice, University of Antwerp Antwerp, Belgium
    J Antimicrob Chemother 58:413-7. 2006
    ..Data on outpatient cephalosporin use in Europe were collected from 25 countries within the ESAC project, funded by DG SANCO of the European Commission, using the WHO ATC/DDD methodology...
  85. ncbi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient penicillin use in Europe
    Matus Ferech
    Laboratory of Microbiology, University of Antwerp Antwerp, Belgium
    J Antimicrob Chemother 58:408-12. 2006
    ..Data on outpatient penicillin use in Europe were collected from 25 countries within the ESAC project, funded by DG SANCO of the European Commission, using the WHO ATC/DDD methodology...
  86. ncbi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient antibiotic use in Europe
    Matus Ferech
    Laboratory of Microbiology, Department of General Practice, University of Antwerp Antwerp, Belgium
    J Antimicrob Chemother 58:401-7. 2006
    ..Data on outpatient antibiotic use were collected from 34 countries using the ATC/DDD methodology...
  87. ncbi request reprint A potential virulence gene, hylEfm, predominates in Enterococcus faecium of clinical origin
    Louis B Rice
    Research and Medical Services, Louis Stokes Cleveland Veterans Affairs Medical Center and Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    J Infect Dis 187:508-12. 2003
    ..Differences in the prevalence of these strains may help explain variations in the clinical importance of multiresistant E. faecium across different continents...
  88. pmc Comparison and evaluation of real-time PCR, real-time nucleic acid sequence-based amplification, conventional PCR, and serology for diagnosis of Mycoplasma pneumoniae
    Kate E Templeton
    Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
    J Clin Microbiol 41:4366-71. 2003
    ..Molecular methods are superior for diagnosis of M. pneumoniae, providing more timely diagnosis. In addition, using real-time methods involves less hands-on time and affords the ability to monitor the reaction in the same tube...
  89. pmc Evaluation of new broth media for microdilution antibiotic susceptibility testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria
    Ingo Klare
    Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, D 38855 Wernigerode, Germany
    Appl Environ Microbiol 71:8982-6. 2005
    ....
  90. ncbi request reprint The more antibacterial trade names, the more consumption of antibacterials: a European study
    Dominique L Monnet
    National Center for Antimicrobials and Infection Control, Statens Serum Institut, Copenhagen, Denmark
    Clin Infect Dis 41:114-7. 2005
    ..This relationship was observed in countries in which consumption was high, as well as in countries in which it was low, and in markets that were opened or were not opened to the sale of copies of original agents...
  91. ncbi request reprint Association between antibiotic sales and public campaigns for their appropriate use
    Isabelle Bauraind
    JAMA 292:2468-70. 2004
  92. ncbi request reprint Antibiotic therapy for Klebsiella pneumoniae bacteremia: implications of production of extended-spectrum beta-lactamases
    David L Paterson
    Infectious Disease Section, VA Medical Center, Pittsburgh, Pennsylvania 15240, USA
    Clin Infect Dis 39:31-7. 2004
    ..Antibiotic choice is particularly important in seriously ill patients with infections due to ESBL-producing K. pneumoniae...
  93. ncbi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient macrolide, lincosamide and streptogramin (MLS) use in Europe
    Samuel Coenen
    Department of General Practice, University of Antwerp Antwerp, Belgium
    J Antimicrob Chemother 58:418-22. 2006
    ..Data on outpatient macrolide, lincosamide and streptogramin (MLS) use in Europe were collected from 25 countries within the ESAC project, funded by DG SANCO of the European Commission, using the WHO ATC/DDD methodology...
  94. ncbi request reprint European Surveillance of Antimicrobial Consumption (ESAC): outpatient quinolone use in Europe
    Matus Ferech
    Laboratory of Microbiology, University of Antwerp Antwerp, Belgium
    J Antimicrob Chemother 58:423-7. 2006
    ..Data on outpatient quinolone use in Europe were collected from 25 countries within the ESAC project, funded by DG SANCO of the European Commission, using the WHO ATC/DDD methodology...
  95. ncbi request reprint Surveillance of outpatient antibiotic consumption in Spain according to sales data and reimbursement data
    Jose Campos
    Antibiotic Laboratory, Bacteriology Service, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    J Antimicrob Chemother 60:698-701. 2007
    ..National data on antibiotic consumption are needed in order to interpret trends in antibiotic resistance. We compared antibiotic prescription reimbursement data and sales data in Spain...
  96. ncbi request reprint Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use
    Ingo Klare
    Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany
    J Antimicrob Chemother 59:900-12. 2007
    ..To propose tentative epidemiological cut-off (ECOFF) values for recognizing intrinsic and acquired antimicrobial resistances in numerically dominant species...
  97. ncbi request reprint Comment on: Can mass media campaigns change antimicrobial prescribing? A regional evaluation study
    Samuel Coenen
    J Antimicrob Chemother 60:179-80. 2007
  98. ncbi request reprint International prospective study of Klebsiella pneumoniae bacteremia: implications of extended-spectrum beta-lactamase production in nosocomial Infections
    David L Paterson
    Veterans Affairs Medical Center, Pittsburgh, Pennsylvania 15240, USA
    Ann Intern Med 140:26-32. 2004
    ..Commonly encountered nosocomially acquired gram-negative bacteria, especially Klebsiella pneumoniae, produce extended-spectrum beta-lactamases (ESBLs) as an antibiotic resistance mechanism...
  99. pmc Virulence characteristics of Klebsiella and clinical manifestations of K. pneumoniae bloodstream infections
    Victor L Yu
    University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Emerg Infect Dis 13:986-93. 2007
    ..001). Differences in clinical features of bacteremic infection with K. pneumoniae are due to the virulence factors expressed by the organism...
  100. pmc Antibiotics for respiratory tract infections in primary care
    Samuel Coenen
    BMJ 335:946-7. 2007
  101. pmc Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis
    Kate E Templeton
    Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    J Clin Microbiol 41:4121-6. 2003
    ..The real-time PCR designed for B. pertussis and B. parapertussis provides sensitive and specific diagnosis of B. pertussis and B. parapertussis infections and is therefore suitable for implementation in the diagnostic laboratory...