Research Topics
| P E J BolsSummaryAffiliation: University of Antwerp Country: Belgium Publications
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Detail Information
Publications
A comparison of a mechanical sector and a linear array transducer for ultrasound-guided transvaginal oocyte retrieval (OPU) in the cowP E J Bols
Laboratory of Veterinary Physiology, Faculty of Farmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Gebouw U, 2610 Wilrijk, Belgium
Theriogenology 62:906-14. 2004..In conclusion, both systems can be effectively used for oocyte retrieval in the cow, however, the MAP transducer demonstrated superior visualization of small follicles...
Xenotransplantation in immunodeficient mice to study ovarian follicular development in domestic animalsP E J Bols
Laboratory of Veterinary Physiology, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Wilrijk, Belgium
Theriogenology 73:740-7. 2010..Different grafting strategies will be discussed as well as the consequences of this procedure on the viability and dynamic behavior of the grafted tissue and follicles...
Unravelling the needs of singly in vitro-produced bovine embryos: from cumulus cell co-culture to semi-defined, oil-free culture conditionsI G F Goovaerts
University of Antwerp, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Veterinary Physiology, Universiteitsplein 1, Gebouw U, B 2610 Wilrijk, Belgium
Reprod Fertil Dev 24:1084-92. 2012..This research additionally stresses the fact that specific interaction mechanisms between somatic cells and a developing in vitro embryo are far from unravelled...
Intrafollicular conditions as a major link between maternal metabolism and oocyte quality: a focus on dairy cow fertilityJ L M R Leroy
Laboratory for Veterinary Physiology, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B 2610 Wilrijk, Belgium
Reprod Fertil Dev 24:1-12. 2011..Finally, there is growing evidence that adverse conditions for oocyte growth and maturation may also jeopardise the health and performance of the offspring...
Single in vitro bovine embryo production: coculture with autologous cumulus cells, developmental competence, embryo quality and gene expression profilesI G F Goovaerts
University of Antwerp, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Wilrijk, Belgium
Theriogenology 76:1293-303. 2011....
Roles of lipid peroxidation and cytoplasmic droplets on in vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34 degrees CM Nichi
University of Antwerp, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Veterinary Physiology, Universiteitsplein 1, Gebouw U, B 2610 Wilrijk, Belgium
Theriogenology 67:334-40. 2007..We hypothesize that this protection against ROS is one of the functions of distal sperm droplets in the epididymis...
Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part I. The importance of negative energy balance and altered corpus luteum function to the reduction of oocyte and embryo quality in high-yielding dairy cowsJ L M R Leroy
Laboratory for Veterinary Physiology, Department of Veterinary Sciences, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Wilrijk, Belgium
Reprod Domest Anim 43:612-22. 2008..This may partly account for the low conception rates and the high incidence of early embryonic mortality in high yielding dairy cows...
Quantification and viability assessment of isolated bovine primordial and primary ovarian follicles retrieved through a standardized biopsy pick-up procedureJ M J Aerts
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium
Reprod Domest Anim 43:360-6. 2008..We conclude that the standardized BPU procedure generates sufficiently large numbers of vital primordial and primary follicles, thus validating BPU as a new tool for research into early bovine follicular development...
Effect of cumulus cell coculture and oxygen tension on the in vitro developmental competence of bovine zygotes cultured singlyI G F Goovaerts
University of Antwerp, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Veterinary Physiology, Universiteitsplein 1, Gebouw U, B 2610 Wilrijk, Belgium
Theriogenology 71:729-38. 2009..These results were consistently achieved after acquiring experience and expertise in the handling of single zygotes...
Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditionsM Nichi
University of Antwerp, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Veterinary Physiology, Universiteitsplein 1, Gebouw U, B 2610 Wilrijk, Belgium
Theriogenology 66:822-8. 2006....
Endocrine disruptors and female fertility: focus on (bovine) ovarian follicular physiologyE M L Petro
Gamete Research Center, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium
Theriogenology 78:1887-900. 2012..In this review, we explore how EDCs can interfere with the well-balanced conditions in the ovarian follicle. In addition, we highlight the bovine ovarian follicle as an alternative in vitro model for EDC and broader toxicology research...
181 xenotransplantation of bovine ovarian cortex in severe combined immunodeficient mice to study pre-antral follicular development: determination of the optimal graft siteA Langbeen
University of Antwerp, Wilrijk, Antwerp, Belgium
Reprod Fertil Dev 25:239-40. 2012....
94 a polarized cell-culture system to study the effects of elevated serum nonesterified Fatty Acid concentrations on the bovine oviductal microenvironment in vitroL Jordaens
University of Antwerp, Wilrijk, Antwerp, Belgium
Reprod Fertil Dev 25:195. 2012..Further research should focus on transport and metabolisation of the fatty acids added...
Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part II. Mechanisms linking nutrition and reduced oocyte and embryo quality in high-yielding dairy cowsJ L M R Leroy
Laboratory for Veterinary Physiology, Department of Veterinary Sciences, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Wilrijk, Belgium
Reprod Domest Anim 43:623-32. 2008..Several nutrition-linked mechanisms, through which oocyte and/or embryo quality can be affected in modern dairy cows, well after the period of NEB, are proposed and comprehensively reviewed in the present report...
Noninvasive bovine oocyte quality assessment: possibilities of a single oocyte cultureI G F Goovaerts
University of Antwerp, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Veterinary Physiology, Wilrijk, Belgium
Theriogenology 74:1509-20. 2010....
The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo qualityJ L M R Leroy
Laboratory for Veterinary Physiology, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B 2610 Wilrijk, Belgium
Hum Reprod 25:768-78. 2010..We aimed to induce hyperlipidaemia nutritionally and investigate the consequences of hyperlipidaemic culture conditions on bovine in vitro embryo development, embryo quality and gene expression patterns...
The effect of animal handling procedures on the blood non-esterified fatty acid and glucose concentrations of lactating dairy cowsJ L M R Leroy
Laboratory for Veterinary Physiology, Department of Veterinary Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Wilrijk B 2610, Belgium
Vet J 187:81-4. 2011..The findings suggest that handling lactating cows prior to blood-sampling should be minimised in order to reduce the effect on blood glucose and NEFA concentrations and possible consequent misinterpretation of the animals' energy status...
Oocyte developmental failure in response to elevated nonesterified fatty acid concentrations: mechanistic insightsV Van Hoeck
Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, Gamete Research Center, University of Antwerp, Universiteitsplein 1 Gebouw U, B 2610 Wilrijk, Belgium
Reproduction 145:33-44. 2013....
139 the effect of starch and saturated or polyunsaturated rich diets on in vitro bovine embryo development and qualityV Van Hoeck
University of Antwerp, Wilrijk, Antwerp, Belgium
Reprod Fertil Dev 25:217. 2012..Changing the fat source to polyunsaturated eliminated these negative effects. Furthermore, we showed that the order in which different fat types are fed affects the zygote's ability to sustain further development...
Ovarian follicular dynamics: a review with emphasis on the bovine species. Part I: Folliculogenesis and pre-antral follicle developmentJ M J Aerts
Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium
Reprod Domest Anim 45:171-9. 2010..Finally, an in-depth discussion of pre-antral follicle development engages in the current hypotheses regarding primordial follicle activation, and the role of gonadotrophins and angiogenesis...
Ovarian follicular dynamics. A review with emphasis on the bovine species. Part II: Antral development, exogenous influence and future prospectsJ M J Aerts
Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, Belgium
Reprod Domest Anim 45:180-7. 2010..The next section expounds upon the mechanisms for exogenous modulation of follicular dynamics with the aim of superovulation/superstimulation, and finally prospective future research directions are sketched...
Nutrient prioritization in dairy cows early postpartum: mismatch between metabolism and fertility?J L M R Leroy
Veterinary Centre Someren, Someren, The Netherlands
Reprod Domest Anim 43:96-103. 2008..All this, with one question in mind: is there a mismatch between metabolism and fertility and what can the dairy manager learn from research to tackle the problem of reduced fertility?..
5 the effect of elevated nonesterified Fatty Acid concentrations on murine in vitro folliculogenesis and subsequent oocyte developmental competenceS Valckx
University of Antwerp, Wilrijk, Antwerp, Belgium
Reprod Fertil Dev 25:150. 2012..Ongoing research focuses on the pathways involved...
The consequences of metabolic changes in high-yielding dairy cows on oocyte and embryo qualityJ L M R Leroy
1Laboratory for Veterinary Physiology, Department of Veterinary Sciences, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B 2610 Wilrijk, Belgium
Animal 2:1120-7. 2008..This important observation may be linked to the typical diet provided to stimulate milk yield, or to physiological adaptations sustaining the high milk production. Research into this phenomenon is ongoing...
Evaluation of epididymal semen quality using the Hamilton-Thorne analyser indicates variation between the two caudae epididymides of the same bullI G F Goovaerts
Laboratory of Veterinary Physiology, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Gebouw U, B 2610 Wilrijk, Belgium
Theriogenology 66:323-30. 2006....
BMI-related metabolic composition of the follicular fluid of women undergoing assisted reproductive treatment and the consequences for oocyte and embryo qualityS D M Valckx
Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, 2610 Wilrijk, Belgium
Hum Reprod 27:3531-9. 2012....
Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP systemS A Chaubal
Center for Regenerative Biology, University of Connecticut, 1392 Storrs Road, Storrs, CT 06269 4243, USA
Theriogenology 67:719-28. 2007..05) available follicles for aspiration. Moreover, when ovarian stimulation in the absence of CIDR was followed by administration of LH 6h prior to OPU, it increased (P<0.05) the number of blastocysts per OPU session...
Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cowsS A Chaubal
Center for Regenerative Biology/Department of Animal Science, University of Connecticut, 1392 Storrs Rd, Storrs, CT 06269-4243, USA
Theriogenology 65:1631-48. 2006..However, the protocol that consisted of DFR, FSH treatment and a subsequent single OPU per week, was the most productive and cost-effective, with potential commercial appeal...
Characterisation of low, medium and high responders following FSH stimulation prior to ultrasound-guided transvaginal oocyte retrieval in cowsR De Roover
Universite de Liege, Faculte de Medecine Veterinaire, Service d Obstétrique et de Pathologie de la Reproduction des ruminants, équidés et porcs, B42 Sart Tilman, B 4000 Liege, Belgium
Theriogenology 63:1902-13. 2005..Selection of animals with extreme (high or low) follicle and COC production results allows us to distinguish three populations: 'low, medium, and high responders' to FSH stimulation...
Comparison of embryo quality in high-yielding dairy cows, in dairy heifers and in beef cowsJ L M R Leroy
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, B 9820 Merelbeke, Belgium
Theriogenology 64:2022-36. 2005..Producing milk or not seems to be significantly associated with embryo quality. Therefore, reduced embryo quality on Day 7 following AI, could be an important factor in the subfertility problem in modern high-yielding dairy cows...
Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post partumJ L M R Leroy
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
Theriogenology 62:1131-43. 2004....
Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo productionR De Roover
Faculte de Medecine Veterinaire, Service d Obstétrique et de Pathologie de la Reproduction des ruminants, équidés et porcs, Universite de Liege, Sart Tilman, Boulevard de Colonster 20, 4000 Liege, Belgium
Reprod Domest Anim 43:239-45. 2008....
Puncture of immature ovarian follicles in bovine assisted reproductionP E J Bols
Verh K Acad Geneeskd Belg 67:177-202. 2005....
Metabolite and ionic composition of follicular fluid from different-sized follicles and their relationship to serum concentrations in dairy cowsJ L M R Leroy
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke B 9820, Belgium
Anim Reprod Sci 80:201-11. 2004....
