Research Topics
| I LiebaersSummaryAffiliation: Universitair Ziekenhuis Brussel Country: Belgium Publications
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Detail Information
Publications
Generation of lung epithelial-like tissue from human embryonic stem cellsLindsey Van Haute
Department of Embryology and Genetics, UZ Brussel, Belgium
Respir Res 10:105. 2009..The aim of the present study was to investigate the effect of specific culture conditions on the differentiation of hESC into lung epithelial cells...
Report on a consecutive series of 581 children born after blastomere biopsy for preimplantation genetic diagnosisI Liebaers
Centre for Medical Genetics, Universitair Ziekenhuis Brussel, Laarbeeklaan 101 1090, Brussels, Belgium
Hum Reprod 25:275-82. 2010..Although healthy children post-PGD/PGS have been born, the question of whether embryo biopsy could have any harmful effects has to be studied on large series in a prospective manner...
Preimplantation diagnosis for fragile X syndrome based on the detection of the non-expanded paternal and maternal CGGK Sermon
Centre for Medical Genetics, University Hospital and Medical School, Dutch Speaking Brussels Free University, Belgium
Prenat Diagn 19:1223-30. 1999..Our candidate-patient population, as well as two clinical preimplantation genetic diagnosis (PGD) cycles which led to a pregnancy with an unaffected fetus, are presented in this paper...- PGD in the lab for triplet repeat diseases - myotonic dystrophy, Huntington's disease and Fragile-X syndromeK Sermon
Centre for Medical Genetics, Dutch Speaking Brussels Free University, University Hospital and Medical School, Laarbeeklaan 101, 1090, Brussels, Belgium
Mol Cell Endocrinol 183:S77-85. 2001..We describe here our experiences from 1995 to May 2000 with PGD for these three triplet repeat diseases... - Real and expected delivery rates of patients with myotonic dystrophy undergoing intracytoplasmic sperm injection and preimplantation genetic diagnosisW Verpoest
Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, UZ Brussel, 101 Laarbeeklaan, B 1090 Brussel, Belgium
Hum Reprod 23:1654-60. 2008..The secondary outcome parameter of this study was ovarian response as a way to express gonadal function in female DM1 patients... - Chromosomal aneuploidy in embryos conceived with unstimulated cycle IVFW Verpoest
Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, 101 Laarbeeklaan, B 1090 Brussels, Belgium
Hum Reprod 23:2369-71. 2008..Two healthy girls were born and one patient miscarried. Numerical chromosomal abnormalities (aneuploidy) are present even in embryos of young women, and in the absence of ovarian stimulation... - PGD for autosomal dominant polycystic kidney disease type 1M De Rycke
Centre for Medical Genetics, University Hospital and Medical School, Dutch Speaking Brussels Free University, Laarbeeklaan, Brussels, Belgium
Mol Hum Reprod 11:65-71. 2005..4% allele drop-out and 4.3% contamination), and can be applied in all informative ADPKD type 1 couples. From five clinical cycles carried out for three couples, two pregnancies ensued, resulting in the birth of two healthy children... - Single embryo transfer in preimplantation genetic diagnosis cycles for women <36 years does not reduce delivery rateP Donoso
Centre for Reproductive Medicine, University Hospital, Dutch Speaking Brussels Free University Vrije Universiteit Brussel, Brussels, Belgium
Hum Reprod 22:1021-5. 2007..The aim of this study is to assess the impact of this legislation on the outcome of preimplantation genetic diagnosis (PGD) for inherited diseases in young women undergoing SET... - Cumulative reproductive outcome after preimplantation genetic diagnosis: a report on 1498 couplesW Verpoest
Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, 101 Laarbeeklaan, B 1090 Brussel, Belgium
Hum Reprod 24:2951-9. 2009..The aim of this study was to analyse the cumulative reproductive outcome of a large cohort of couples undergoing PGD in relation to a number of explanatory variables potentially affecting the prognosis... - The incidence of monozygotic twinning following PGD is not increasedW Verpoest
Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, 101 Laarbeeklaan, B 1090 Brussel, Belgium
Hum Reprod 24:2945-50. 2009..We compared the incidence of MZ twin pregnancies in ICSI cycles with PGD, versus ICSI cycles without PGD... - Neonatal follow-up of 995 consecutively born children after embryo biopsy for PGDS Desmyttere
Centre for Medical Genetics, UZ Brussel, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium
Hum Reprod 27:288-93. 2012..The objective of this study was to determine whether embryo biopsy as performed in PGD has an impact on the health of infants up to 2 months of age... - Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfersA De Vos
Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, B 1090 Brussels, Belgium
Hum Reprod 24:2988-96. 2009..Human embryo biopsy is performed for preimplantation genetic diagnosis (PGD). The impact of 1- or 2-cell removal at cleavage-stage on future embryo development and implantation capacity is highly debated... - Preimplantation genetic screening does not improve delivery rate in women under the age of 36 following single-embryo transferC Staessen
Centre for Medical Genetics, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium
Hum Reprod 23:2818-25. 2008..Besides the morphological quality and embryo kinetics up to the blastocyst stage, preimplantation genetic screening (PGS) of aneuploidy has been advocated as an adjuvant approach to select the embryo... - Novel universal approach for preimplantation genetic diagnosis of beta-thalassaemia in combination with HLA matching of embryosH Van de Velde
Centre for Reproductive Medicine, Brussels, Belgium
Hum Reprod 19:700-8. 2004..Furthermore, healthy embryos could be HLA matched with the affected child so that cord blood from the future newborn can be used to transplant the affected sibling... - Medical outcome of 8-year-old singleton ICSI children (born >or=32 weeks' gestation) and a spontaneously conceived comparison groupF Belva
AZ VUB Medical Genetics, Brussels, Belgium
Hum Reprod 22:506-15. 2007..To investigate possible health problems, a thorough medical and neurological examination was performed... - Preimplantation genetic diagnosis for sickle-cell anemia and for beta-thalassemiaM De Rycke
Centre for Medical Genetics, University Hospital of the Dutch speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium
Prenat Diagn 21:214-22. 2001..The patient with the fluorescent PCR-PGD cycle became pregnant. Overall, the three fluorescent PCR assays were accurate and reliable with amplification efficiencies of minimum 93% and allele dropout (ADO) rates between 0 and 12%... - GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditionsU Ullmann
Department of Embryology and Genetics, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel VUB, Laarbeeklaan 101, 1090 Brussels, Belgium
Mol Hum Reprod 14:169-79. 2008..This study showed that BIO, a GSK-3-specific inhibitor, prevents the EMT process which is associated with the feeder-free hESC culture. Nevertheless, BIO was not sufficient to expand hESCs in a long-term culture system... - Preimplantation genetic diagnosis for medium-chain acyl-CoA dehydrogenase (MCAD) deficiencyK Sermon
Centre for Medical Genetics, and Centre for Reproductive Medicine, University Hospital and Medical School of the Dutch speaking Brussels Free University Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium
Mol Hum Reprod 6:1165-8. 2000..The patient became pregnant after one PGD cycle during which two embryos were replaced. The twin pregnancy was checked by chorionic villus sampling (CVS) and was shown to be unaffected. The twins have been born and are healthy... - PGD in 47,XXY Klinefelter's syndrome patientsC Staessen
Centre for Reproductive Medicine, University Hospital, Dutch speaking Free University of Brussels Vrije Universiteit Brussel, Brussels, Belgium
Hum Reprod Update 9:319-30. 2003..Hence, a cautious approach is warranted in advising couples with non-mosaic Klinefelter's syndrome. Moreover, the use of ICSI + PGD or prenatal diagnosis should be carefully considered... - Cerebral palsy and pyruvate dehydrogenase deficiency: identification of two new mutations in the E1alpha geneW Lissens
Department of Paediatric Neurology, University Hospital, Vrije Universiteit Brussel, Brussels, Belgium
Eur J Pediatr 158:853-7. 1999..CONCLUSION: Mutation identification in pyruvate dehydrogenase complex deficiency remains important especially for the determination of the recurrence risk and for reliable genetic counselling in couples with an affected child... - A mutation (IVS8+0.6kbdelTC) creating a new donor splice site activates a cryptic exon in an Alu-element in intron 8 of the human beta-glucuronidase geneR Vervoort
Department of Medical Genetics, University Hospital, Vrije Universiteit Brussel, Brussels, Belgium
Hum Genet 103:686-93. 1998..This is the third report of a human disease mutation that creates a splice site and activates an antisense Alu-cassette; the question rises as to how these apparently strong cryptic exons are generally excluded from coding sequences... - Optimization and evaluation of single-cell whole-genome multiple displacement amplificationC Spits
Research Centre for Reproduction and Genetics, Academisch Ziekenhuis, Vrije Universiteit Brussel, Brussels, Belgium
Hum Mutat 27:496-503. 2006..This new protocol can be useful for many applications involving minute quantities of starting material, such as forensic DNA analysis, prenatal and preimplantation genetic diagnosis, or cancer research... - Mutation analysis of three genes in patients with maturation arrest of spermatogenesis and couples with recurrent miscarriagesK Stouffs
Centre for Medical Genetics, Universitair Ziekenhuis Brussel, Belgium
Reprod Biomed Online 22:65-71. 2011..More research is also necessary to determine the impact and frequency of such changes before implementing mutation screening in genetic counselling... - Epithelial-mesenchymal transition process in human embryonic stem cells cultured in feeder-free conditionsU Ullmann
Research Centre Reproduction and Genetics, University Hospital and Medical School of the Vrije Universiteit Brussel, VUB, Free University of Brussels, Brussels, Belgium
Mol Hum Reprod 13:21-32. 2007..Taken together, our data suggest that culturing hESCs in feeder-free conditions enhances an early differentiation process identified as an EMT... - Developmental outcome at 2 years of age for children born after ICSI compared with children born after IVFM Bonduelle
Centre for Medical Genetics, Department of Developmental Psychology, Dutch Speaking Brussels Free University, Brussels, Belgium
Hum Reprod 18:342-50. 2003..Hence, this outcome was assessed by performing a standard developmental test on children born after ICSI as compared with children born after IVF, at the age of 2 years... - Improving clinical preimplantation genetic diagnosis for cystic fibrosis by duplex PCR using two polymorphic markers or one polymorphic marker in combination with the detection of the DeltaF508 mutationV Goossens
Centre for Medical Genetics, University Hospital and Medical School, Dutch Speaking Brussels Free University, Brussels, Belgium
Mol Hum Reprod 9:559-67. 2003..In total, 22 PGD cycles with duplex PCR (IVS8CA/IVS17BTA, DeltaF508/IVS8CA, DeltaF508/IVS17BTA and D7S486/D7S490) were carried out in 16 couples, which resulted in four ongoing pregnancies and one miscarriage... - Cognitive and motor development of 8-year-old children born after ICSI compared to spontaneously conceived childrenL Leunens
Developmental and Lifespan Psychology, Centre for Medical Genetics, Vrije Universiteit Brussel, Brussels, Belgium
Hum Reprod 21:2922-9. 2006..As a continuation of two large-scale, multicentre studies on the development of 5-year-old ICSI children, we present results of the follow-up study undertaken on the cognitive and motor development of 8-year-old ICSI children... - Characterization of CD30 expression in human embryonic stem cell lines cultured in serum-free media and passaged mechanicallyI Mateizel
Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium
Hum Reprod 24:2477-89. 2009..Here we investigate the expression of CD30, reported to be a biomarker of hESCs with abnormal karyotype, in undifferentiated and spontaneously differentiated hESC... - Markers that define stemness in ESC are unable to identify the totipotent cells in human preimplantation embryosG Cauffman
Department of Embryology and Genetics, UZ Brussel and the Vrije Universiteit Brussel, Laarbeeklaan 101, Brussels 1090, Belgium
Hum Reprod 24:63-70. 2009..KRT18, a trophoblast-determining gene, may mark early differentiation. Examining the expression pattern of these genes may inform us about when and in which cells totipotency is lost during early human development... - Neonatal outcome of 724 children born after ICSI using non-ejaculated spermF Belva
Center for Medical Genetics, UZ Brussel, Brussels 1090, Belgium
Hum Reprod 26:1752-8. 2011..Therefore, adverse health outcomes, birth parameters, major anomaly rates and chromosomal aberrations in children born after ICSI using testicular and epididymal sperm were investigated... - Methylation of the CpG sites in the myotonic dystrophy locus does not correlate with CTG expansion size or with the congenital form of the diseaseC Spits
Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, Brussels 1090, Belgium
J Med Genet 47:700-3. 2010..The size range of the repeat expansions with methylation was from as small as 300 to as large as 2800 repeats... - Closed blastocyst vitrification of biopsied embryos: evaluation of 100 consecutive warming cyclesL Van Landuyt
Centre for Reproductive Medicine, UZ Brussel, Laarbeeklaan 101, 1090 Jette, Belgium
Hum Reprod 26:316-22. 2011..The aim of this study was to analyse the outcome of closed blastocyst vitrification of embryos biopsied at the cleavage stage... - Epigenetic risks related to assisted reproductive technologies: risk analysis and epigenetic inheritanceM De Rycke
Centre for Medical Genetics, University Hospital of the Dutch speaking Brussels Free University, Laarbeeklaan 101, Belgium
Hum Reprod 17:2487-94. 2002..The interference of in-vitro embryo culture, immature sperm cells and nuclear transfer with epigenetic reprogramming is discussed, as well as the possibility of epigenetic inheritance... - Outcome of twin gestations after first trimester chorionic villus samplingL De Catte
Feto maternal Medicine Unit, Department of Obstetrics and Gynecology, Academisch Ziekenhuis VUB, Vrije Universiteit, Brussels, Belgium
Obstet Gynecol 96:714-20. 2000..To tabulate genetic results and obstetric outcomes of twin pregnancies after first-trimester chorionic villus sampling (CVS)... - Mutations in the X-linked pyruvate dehydrogenase (E1) alpha subunit gene (PDHA1) in patients with a pyruvate dehydrogenase complex deficiencyW Lissens
Center for Medical Genetics, University Hospital, Vrije Universiteit Brussel, Brussels, Belgium
Hum Mutat 15:209-19. 2000..The mutation was never present in the somatic cells of the father; in 63 mothers studied, 16 were carriers (25%). In four families, the origin of the new mutation was determined to be twice paternal and twice maternal... - Intracytoplasmic sperm injectionAndre Van Steirteghem
Center for Reproductive Medicine, University Hospital and Medical School, Brussels Free University, Laarbeeklaan 101, B 1090, Brussels, Belgium
Mol Cell Endocrinol 186:199-203. 2002..Important outcome data to be examined comprise information on major and minor congenital malformations obtained prenatally or after birth, as well as on the further development of the children...