Claire Remacle

Summary

Country: Belgium

Publications

  1. doi request reprint Atypical subunit composition of the chlorophycean mitochondrial F1FO-ATP synthase and role of Asa7 protein in stability and oligomycin resistance of the enzyme
    Marie Lapaille
    Genetics of Microorganisms, Department of Life Sciences, Universite de Liege, Belgium
    Mol Biol Evol 27:1630-44. 2010
  2. doi request reprint Eukaryotic complex I: functional diversity and experimental systems to unravel the assembly process
    Claire Remacle
    Genetics of Microorganisms Laboratory, Department of Life Sciences, University of Liege, Liege, Belgium
    Mol Genet Genomics 280:93-110. 2008
  3. pmc High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes
    Claire Remacle
    Génétique des Microorganismes, Departement des Sciences de la Vie, Institut de Botanique B22, Universite de Liege, B 4000 Liege, Belgium
    Proc Natl Acad Sci U S A 103:4771-6. 2006
  4. doi request reprint Knock-down of the COX3 and COX17 gene expression of cytochrome c oxidase in the unicellular green alga Chlamydomonas reinhardtii
    Claire Remacle
    Department of Life Sciences, Institute of Botany, B22 University of Liege, 4000 Liege, Belgium
    Plant Mol Biol 74:223-33. 2010
  5. ncbi request reprint Impact of a mutation in the mitochondrial LSU rRNA gene from Chlamydomonas reinhardtii on the activity and the assembly of respiratory-chain complexes
    C Remacle
    Department of Life Sciences, Institute of Botany B22, University of Liege, Sart Tilman, 4000, Liege, Belgium
    Curr Genet 45:323-30. 2004
  6. ncbi request reprint Mutations inactivating mitochondrial genes in Chlamydomonas reinhardtii
    C Remacle
    Genetics of Microorganisms, Department of Plant Biology B22, University of Liege, B 4000 Liege, Belgium
    Biochem Soc Trans 29:442-6. 2001
  7. ncbi request reprint Structure of the telomeric ends of mt DNA, transcriptional analysis and complex I assembly in the dum24 mitochondrial mutant of Chlamydomonas reinhardtii
    F Duby
    Department of Plant Biology, University of Liege, Belgium
    Mol Genet Genomics 266:109-14. 2001
  8. doi request reprint Function of the chloroplastic NAD(P)H dehydrogenase Nda2 for H₂ photoproduction in sulphur-deprived Chlamydomonas reinhardtii
    Emmanuel Mignolet
    Laboratory of Bioenergetics, Institute of Plant Biology B22, University of Liege, Belgium
    J Biotechnol 162:81-8. 2012
  9. doi request reprint Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1
    Gregory Mathy
    Laboratory of Bioenergetics and Cellular Physiology, University of Liege, Belgium
    J Proteome Res 9:2825-38. 2010
  10. pmc ND3 and ND4L subunits of mitochondrial complex I, both nucleus encoded in Chlamydomonas reinhardtii, are required for activity and assembly of the enzyme
    Pierre Cardol
    Biochemistry and Photobiology Laboratory, Department of Life Sciences, Universite de Liege, B 4000, Liege, Belgium
    Eukaryot Cell 5:1460-7. 2006

Collaborators

  • Pierre Cardol
  • Bart Ghysels
  • R F Matagne
  • Adrian Reyes-Prieto
  • Marc Hanikenne
  • Gilles Peltier
  • Götz Hofhaus
  • M Havaux
  • Benedicte Mousson de Camaret
  • Marie Lapaille
  • Fabrice Franck
  • Patrick Motte
  • Nathalie Bonnefoy
  • Helene Vigeolas
  • Renaud Lecler
  • Véronique Larosa
  • Nadine Coosemans
  • Emmanuel Mignolet
  • Thalia Salinas
  • Diego González-Halphen
  • Francéline Duby
  • Gregory Mathy
  • Laurence Maréchal-Drouard
  • Frédéric Jans
  • Valerie Cognat
  • Miriam Vázquez-Acevedo
  • Vinciane Tillemans
  • Ingrid Bourges
  • Esra Kaymak
  • Barbara Emonds-Alt
  • Guillaume Niessen
  • F Duby
  • Arnaud Blomme
  • Elizabeth Rodríguez-Salinas
  • Marie Cloes
  • Monique Dinant
  • Stéphanie Gérin
  • Francis E Sluse
  • Marc Boutry
  • Edwin Depauw
  • Adelma Escobar-Ramírez
  • Claudine Sluse-Goffart
  • Marc Thiry
  • Denis Baurain
  • Emilie Perez
  • Pierre Leprince
  • Hervé Degand
  • Elizaveta Vinogradova
  • Pierre Alain Houyoux
  • Laurent Cournac
  • Stéphan Cuiné
  • Jean Marc Deragon
  • Thomas D Fox
  • Araceli Cano-Estrada
  • Laurence Dispa
  • Mélanie Collinge
  • Jean Paul Issartel
  • Claire Ramus
  • Benedicte Mousson de Camaret
  • Réjane Beugnot

Detail Information

Publications28

  1. doi request reprint Atypical subunit composition of the chlorophycean mitochondrial F1FO-ATP synthase and role of Asa7 protein in stability and oligomycin resistance of the enzyme
    Marie Lapaille
    Genetics of Microorganisms, Department of Life Sciences, Universite de Liege, Belgium
    Mol Biol Evol 27:1630-44. 2010
    ..In these respects, we discuss an evolutionary scenario for F(1)F(O)-ATP synthase in the whole green lineage (i.e., Chlorophyta and Streptophyta)...
  2. doi request reprint Eukaryotic complex I: functional diversity and experimental systems to unravel the assembly process
    Claire Remacle
    Genetics of Microorganisms Laboratory, Department of Life Sciences, University of Liege, Liege, Belgium
    Mol Genet Genomics 280:93-110. 2008
    ..Moreover, mitochondrial transformation, a recent technological advance, is now available, allowing the manipulation of all five complex I mitochondrial genes in this organism...
  3. pmc High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes
    Claire Remacle
    Génétique des Microorganismes, Departement des Sciences de la Vie, Institut de Botanique B22, Universite de Liege, B 4000 Liege, Belgium
    Proc Natl Acad Sci U S A 103:4771-6. 2006
    ....
  4. doi request reprint Knock-down of the COX3 and COX17 gene expression of cytochrome c oxidase in the unicellular green alga Chlamydomonas reinhardtii
    Claire Remacle
    Department of Life Sciences, Institute of Botany, B22 University of Liege, 4000 Liege, Belgium
    Plant Mol Biol 74:223-33. 2010
    ..Concerning Cox3, its role in Chlamydomonas complex IV is similar to that of other eukaryotes although this subunit is encoded in the nuclear genome in the alga contrary to the situation found in all other organisms...
  5. ncbi request reprint Impact of a mutation in the mitochondrial LSU rRNA gene from Chlamydomonas reinhardtii on the activity and the assembly of respiratory-chain complexes
    C Remacle
    Department of Life Sciences, Institute of Botany B22, University of Liege, Sart Tilman, 4000, Liege, Belgium
    Curr Genet 45:323-30. 2004
    ..We moreover showed that m did not suppress other frameshift or UGA stop mutations which affect mitochondrial genes...
  6. ncbi request reprint Mutations inactivating mitochondrial genes in Chlamydomonas reinhardtii
    C Remacle
    Genetics of Microorganisms, Department of Plant Biology B22, University of Liege, B 4000 Liege, Belgium
    Biochem Soc Trans 29:442-6. 2001
    ..Assembly of complex I in several mutant strains and mapping of mitochondrial mutations by recombinational analysis are also described...
  7. ncbi request reprint Structure of the telomeric ends of mt DNA, transcriptional analysis and complex I assembly in the dum24 mitochondrial mutant of Chlamydomonas reinhardtii
    F Duby
    Department of Plant Biology, University of Liege, Belgium
    Mol Genet Genomics 266:109-14. 2001
    ....
  8. doi request reprint Function of the chloroplastic NAD(P)H dehydrogenase Nda2 for H₂ photoproduction in sulphur-deprived Chlamydomonas reinhardtii
    Emmanuel Mignolet
    Laboratory of Bioenergetics, Institute of Plant Biology B22, University of Liege, Belgium
    J Biotechnol 162:81-8. 2012
    ..Nda2 is therefore important for linking H₂ photoproduction with catabolism of storage carbon compounds, and seems also involved in regulating the redox poise of the photosynthetic electron transport chain during H₂ photoproduction...
  9. doi request reprint Proteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1
    Gregory Mathy
    Laboratory of Bioenergetics and Cellular Physiology, University of Liege, Belgium
    J Proteome Res 9:2825-38. 2010
    ....
  10. pmc ND3 and ND4L subunits of mitochondrial complex I, both nucleus encoded in Chlamydomonas reinhardtii, are required for activity and assembly of the enzyme
    Pierre Cardol
    Biochemistry and Photobiology Laboratory, Department of Life Sciences, Universite de Liege, B 4000, Liege, Belgium
    Eukaryot Cell 5:1460-7. 2006
    ..The putative role of hydrophobic ND subunits is discussed in relation to the structure of the complex I enzyme. A model for the assembly pathway of the Chlamydomonas enzyme is proposed...
  11. doi request reprint Reconstruction of a human mitochondrial complex I mutation in the unicellular green alga Chlamydomonas
    Véronique Larosa
    Genetics of Microorganisms, Department of Life Sciences, Institute of Botany, University of Liege, B 4000 Liege, Belgium
    Plant J 70:759-68. 2012
    ..The in vitro defects correlate with a decrease in dark respiration and growth rate in vivo...
  12. pmc Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas
    Thalia Salinas
    Génétique des Microorganismes, Department of Life Sciences, Institute of Botany, University of Liege, Liege, Belgium
    PLoS Genet 8:e1002946. 2012
    ..It rather suggests that the steady-state levels of imported tRNAs in mitochondria result from a co-evolutive adaptation between the tRNA import mechanism and the requirements of the mitochondrial translation machinery...
  13. ncbi request reprint Impact of mutations affecting ND mitochondria-encoded subunits on the activity and assembly of complex I in Chlamydomonas. Implication for the structural organization of the enzyme
    Pierre Cardol
    Genetics of Microorganisms, Department of Life Sciences, B22, University of Liege, Belgium
    J Mol Biol 319:1211-21. 2002
    ..In mitochondria from all the strains analyzed, we moreover detected a 160-210 kDa fragment comprising the hydrophilic 49 kDa and 76 kDa subunits of the complex I peripheral arm and showing NADH dehydrogenase activity...
  14. doi request reprint Loss of mitochondrial ATP synthase subunit beta (Atp2) alters mitochondrial and chloroplastic function and morphology in Chlamydomonas
    Marie Lapaille
    Laboratoire de génétique des microorganismes, B22, Institut de Botanique, Universite de Liege, B 4000 Liege, Belgium
    Biochim Biophys Acta 1797:1533-9. 2010
    ..Altogether, our results contribute to the understanding of the yet poorly studied bioenergetic interactions between organelles in photosynthetic organisms...
  15. doi request reprint Characterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria
    Renaud Lecler
    Laboratory of Genetics of Microorganisms, Institute of Botany, University of Liege, B22, Boulevard du Rectorat, 27, 4000 Liege, Belgium
    Curr Genet 58:205-16. 2012
    ..Respiration and growth rates in heterotrophic conditions were significantly altered in the double mutants investigated, suggesting that Nda1 plays a role in the oxidation of matrix NADH in the absence of complex I...
  16. pmc The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii
    Claire Remacle
    Department of Life Sciences, Universite de Liege, Liege, Belgium
    Eukaryot Cell 8:1460-3. 2009
    ..Integration of an engineered ARG9 cassette in the plastid chromosome of the nuclear arg9 mutant restores arginine prototrophy. This suggests that ARG9 could be used as a new selectable marker for plastid transformation...
  17. pmc A type II NAD(P)H dehydrogenase mediates light-independent plastoquinone reduction in the chloroplast of Chlamydomonas
    Frédéric Jans
    Laboratorie of Plant Biochemistry and Photobiology, Department of Life Sciences, Institute of Plant Biology, B22, University of Liege, 27 Boulevard du Rectorat, 4000 Liege, Belgium
    Proc Natl Acad Sci U S A 105:20546-51. 2008
    ..These results demonstrate the importance of Nda2 for nonphotochemical PQ reduction and associated processes in C. reinhardtii...
  18. doi request reprint In Chlamydomonas, the loss of ND5 subunit prevents the assembly of whole mitochondrial complex I and leads to the formation of a low abundant 700 kDa subcomplex
    Pierre Cardol
    Genetics of Microorganisms, Department of Life Sciences, B22, University of Liege, B 4000 Liege, Belgium
    Biochim Biophys Acta 1777:388-96. 2008
    ..Finally, a supercomplex most probably corresponding to complex I-complex III association, was identified in mutant mitochondria, indicating that the missing part of the enzyme is not required for the formation of the supercomplex...
  19. doi request reprint Isolation and partial characterization of mutants with elevated lipid content in Chlorella sorokiniana and Scenedesmus obliquus
    Helene Vigeolas
    Laboratory of Genetics of Microorganisms, Department of Life Sciences, Institute of Botany, B22 Boulevard du Rectorat 27, University of Liege, 4000 Liege, Belgium
    J Biotechnol 162:3-12. 2012
    ..Such mutants could subsequently be used as commercial oleaginous algae and serve as an alternative to conventional petrol...
  20. pmc Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration
    Pierre Cardol
    Genetics of Microorganisms, Institute of Plant Biology B22, University of Liege, B 4000 Liege, Belgium
    Plant Physiol 133:2010-20. 2003
    ..The present results demonstrate that in C. reinhardtii mutants, permanent defects in the mitochondrial electron transport chain stabilize state 2, which favors cyclic over linear electron transport in the chloroplast...
  21. pmc The mitochondrial oxidative phosphorylation proteome of Chlamydomonas reinhardtii deduced from the Genome Sequencing Project
    Pierre Cardol
    Genetics of Microorganisms, Institute of Plant Biology B22, University of Liege, B 4000 Liege, Belgium
    Plant Physiol 137:447-59. 2005
  22. ncbi request reprint Higher plant-like subunit composition of mitochondrial complex I from Chlamydomonas reinhardtii: 31 conserved components among eukaryotes
    Pierre Cardol
    Genetics of Microorganisms, Department of Life Sciences, University of Liege, B22, Institute of Botany, B4000 Liege, Belgium
    Biochim Biophys Acta 1658:212-24. 2004
    ..Protein sequence divergence in these kingdom-specific sets is significant and currently we cannot exclude the possibility that homology between them exists, but has not yet been detected...
  23. pmc Mutants of Chlamydomonas reinhardtii deficient in mitochondrial complex I: characterization of two mutations affecting the nd1 coding sequence
    C Remacle
    Laboratory of Genetics of Microorganisms, Department of Plant Biology, , , Belgium
    Genetics 158:1051-60. 2001
    ..A sequencing analysis confirmed this conclusion: dum20 is a deletion of one T at codon 243 of nd1; dum25 corresponds to a 6-bp deletion that eliminates two amino acids located in a very conserved hydrophilic segment of the protein...
  24. ncbi request reprint Functional distribution and dynamics of Arabidopsis SR splicing factors in living plant cells
    Vinciane Tillemans
    Laboratory of Plant Cell and Molecular Biology, University of Liege, B 4000 Liege, Belgium
    Plant J 41:567-82. 2005
    ..SR proteins also localized within small foci at anaphase. These results demonstrate interesting related features as well as potentially important differences between plant and animal SR proteins...
  25. ncbi request reprint The mitochondrial ATP synthase of chlorophycean algae contains eight subunits of unknown origin involved in the formation of an atypical stator-stalk and in the dimerization of the complex
    Miriam Vázquez-Acevedo
    Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Apartado Postal 70 600, Delegacion Coyoacan, 04510, Mexico D F, Mexico
    J Bioenerg Biomembr 38:271-82. 2006
    ..The algal enzyme seems to have modified the structural features of its surrounding scaffold, while conserving almost intact the structure of its catalytic subunits...
  26. pmc Structural organization of mitochondrial human complex I: role of the ND4 and ND5 mitochondria-encoded subunits and interaction with prohibitin
    Ingrid Bourges
    UMR 5090 CNRS DRDC, CEA Grenoble, 38054 Grenoble Cedex 9, France
    Biochem J 383:491-9. 2004
    ..This is the first description of prohibitin interaction with complex I subunits and suggests that this protein might play a role in the assembly or degradation of mitochondrial complex I...
  27. ncbi request reprint Genetic transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii mitochondria
    Nathalie Bonnefoy
    Centre de Genetique Moleculaire, CNRS UPR2167, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Methods Cell Biol 80:525-48. 2007
  28. pmc On the evolution and expression of Chlamydomonas reinhardtii nucleus-encoded transfer RNA genes
    Valerie Cognat
    Institut de Biologie Moleculaire des Plantes, Unité Propre de Recherche 2357, Conventionnée avec l Université Louis Pasteur Strasbourg 1, Centre National de la Recherche Scientifique, 67084 Strasbourg Cedex, France
    Genetics 179:113-23. 2008
    ..Phylogenetic analysis strongly suggests that tRNA(Asp)-related SINEs originate from a prokaryotic-type tRNA either horizontally transferred from a bacterium or originally present in mitochondria or chloroplasts...