K Thevissen

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. pmc The Antifungal Plant Defensin HsAFP1 from Heuchera sanguinea Induces Apoptosis in Candida albicans
    An M Aerts
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven Heverlee, Belgium
    Front Microbiol 2:47. 2011
  2. pmc The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Mol Microbiol 84:166-80. 2012
  3. doi request reprint Novel fungicidal benzylsulfanyl-phenylguanidines
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    Bioorg Med Chem Lett 21:3686-92. 2011
  4. pmc Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    FEMS Microbiol Lett 303:163-8. 2010
  5. ncbi request reprint Antifungal carbazoles
    Karin Thevissen
    CMPG, K U Leuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium
    Curr Med Chem 16:2205-11. 2009
  6. doi request reprint Joined in death: highlights of the Sixth International Meeting on Yeast Apoptosis in Leuven, Belgium, 30 April-4 May 2008
    Karin Thevissen
    Centre for Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium
    Yeast 25:927-34. 2008
  7. ncbi request reprint Ceramide involvement in apoptosis and apoptotic diseases
    Karin Thevissen
    Centre for Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    Mini Rev Med Chem 6:699-709. 2006
  8. ncbi request reprint Fungal sphingolipids as targets for the development of selective antifungal therapeutics
    K Thevissen
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Curr Drug Targets 6:923-8. 2005
  9. pmc A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii)
    K Thevissen
    F A Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Belgium
    Proc Natl Acad Sci U S A 97:9531-6. 2000
  10. ncbi request reprint Fungal membrane responses induced by plant defensins and thionins
    K Thevissen
    F A Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Willem de Croylaan 42, B 3001 Heverlee, Belgium
    J Biol Chem 271:15018-25. 1996

Collaborators

Detail Information

Publications38

  1. pmc The Antifungal Plant Defensin HsAFP1 from Heuchera sanguinea Induces Apoptosis in Candida albicans
    An M Aerts
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven Heverlee, Belgium
    Front Microbiol 2:47. 2011
    ..albicans. These data provide novel mechanistic insights in the mode of action of a plant defensin...
  2. pmc The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Mol Microbiol 84:166-80. 2012
    ..This is the first report on the interaction of a plant defensin with glucosylceramides in the fungal cell wall, causing cell wall stress, and on the effects of a defensin on septin localization and ceramide accumulation...
  3. doi request reprint Novel fungicidal benzylsulfanyl-phenylguanidines
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    Bioorg Med Chem Lett 21:3686-92. 2011
    ..Moreover, addition of those compounds to Candida-infected C. elegans cultures resulted in increased survival of Candida-infected worms, demonstrating their in vivo efficacy in a mini-host model...
  4. pmc Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    FEMS Microbiol Lett 303:163-8. 2010
    ..These data point to a negative regulation of autophagy by both Ipt1 and Skn1 in yeast, with a putative involvement of phytosphingosine in this process...
  5. ncbi request reprint Antifungal carbazoles
    Karin Thevissen
    CMPG, K U Leuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium
    Curr Med Chem 16:2205-11. 2009
    ..In this review, we will further discuss the putative therapeutic potential of the antifungal carbazole compounds as antimycotics...
  6. doi request reprint Joined in death: highlights of the Sixth International Meeting on Yeast Apoptosis in Leuven, Belgium, 30 April-4 May 2008
    Karin Thevissen
    Centre for Microbial and Plant Genetics, Katholieke Universiteit Leuven, Leuven, Belgium
    Yeast 25:927-34. 2008
    ..Together, these data demonstrate the power and advantages of the yeast system to uncover novel cellular factors governing life and death, placing yeast at the forefront of apoptosis research...
  7. ncbi request reprint Ceramide involvement in apoptosis and apoptotic diseases
    Karin Thevissen
    Centre for Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    Mini Rev Med Chem 6:699-709. 2006
    ..Additionally, this review discusses putative therapeutic approaches inhibiting ceramide production in apoptotic diseases...
  8. ncbi request reprint Fungal sphingolipids as targets for the development of selective antifungal therapeutics
    K Thevissen
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Curr Drug Targets 6:923-8. 2005
    ..These compounds include plant and insect defensins, syringomycin E and antifungal antibodies to sphingolipids...
  9. pmc A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii)
    K Thevissen
    F A Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Belgium
    Proc Natl Acad Sci U S A 97:9531-6. 2000
    ..Our data support a model in which membrane patches containing sphingolipids act as binding sites for DmAMP1 or, alternatively, are required to anchor membrane or cell wall-associated proteins, which themselves interact with DmAMP1...
  10. ncbi request reprint Fungal membrane responses induced by plant defensins and thionins
    K Thevissen
    F A Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Willem de Croylaan 42, B 3001 Heverlee, Belgium
    J Biol Chem 271:15018-25. 1996
    ..Our results are consistent with the notion that thionins inhibit fungal growth as a result of direct protein-membrane interactions, whereas plant defensins might act via a different, possibly receptor-mediated, mechanism...
  11. pmc Permeabilization of fungal membranes by plant defensins inhibits fungal growth
    K Thevissen
    F A Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, B 3001 Heverlee Leuven, Belgium
    Appl Environ Microbiol 65:5451-8. 1999
    ..We think that cation-resistant permeabilization is binding site mediated and linked to the primary cause of fungal growth inhibition induced by plant defensins...
  12. ncbi request reprint Ydc1p ceramidase triggers organelle fragmentation, apoptosis and accelerated ageing in yeast
    A M Aerts
    Center of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium
    Cell Mol Life Sci 65:1933-42. 2008
    ..In conclusion, this study describes a direct link between ceramide metabolism in yeast and mitochondrial and vacuolar fragmentation and function, with consequences for chronological lifespan in yeast...
  13. ncbi request reprint SKN1, a novel plant defensin-sensitivity gene in Saccharomyces cerevisiae, is implicated in sphingolipid biosynthesis
    Karin Thevissen
    Centre for Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    FEBS Lett 579:1973-7. 2005
    ..These results show that SKN1, together with IPT1, is involved in sphingolipid biosynthesis in S. cerevisiae...
  14. doi request reprint The mode of antifungal action of plant, insect and human defensins
    A M Aerts
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    Cell Mol Life Sci 65:2069-79. 2008
    ..This review summarizes current knowledge concerning the mode of antifungal action of plant, insect and human defensins...
  15. pmc Membrane rafts are involved in intracellular miconazole accumulation in yeast cells
    Isabelle E J A Francois
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    J Biol Chem 284:32680-5. 2009
    ..We further show that this primary role of membrane rafts in miconazole action consists of mediating intracellular accumulation of miconazole in yeast cells...
  16. ncbi request reprint Currently used antimycotics: spectrum, mode of action and resistance occurrence
    Isabelle E J A Francois
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Curr Drug Targets 6:895-907. 2005
    ..In this review, the mode of action and antifungal spectrum of these classes are discussed, together with possible resistance development against them...
  17. doi request reprint Synthesis and fungicidal activity of 3,5-dichloropyrazin-2(1H)-one derivatives
    Isabelle E J A Francois
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Bioorg Med Chem Lett 19:4064-6. 2009
    ....
  18. ncbi request reprint Genome-wide expression analysis reveals TORC1-dependent and -independent functions of Sch9
    Bart Smets
    Laboratory of Functional Biology, Katholieke Universiteit Leuven, Heverlee, Belgium
    FEMS Yeast Res 8:1276-88. 2008
    ....
  19. ncbi request reprint DmAMP1, an antifungal plant defensin from dahlia (Dahlia merckii), interacts with sphingolipids from Saccharomyces cerevisiae
    Karin Thevissen
    CMPG Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001, Heverlee, Belgium
    FEMS Microbiol Lett 226:169-73. 2003
    ..cerevisiae and that this interaction is enhanced in the presence of equimolar concentrations of ergosterol. Therefore, DmAMP1 antifungal action involving membrane interaction with sphingolipids and ergosterol is proposed...
  20. doi request reprint Design and synthesis of a series of piperazine-1-carboxamidine derivatives with antifungal activity resulting from accumulation of endogenous reactive oxygen species
    Isabelle E Fran├žois
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Heverlee Belgium
    ChemMedChem 4:1714-21. 2009
    ..Moreover, we could link the fungicidal mode of action of the piperazine-1-carboxamidine derivatives to the accumulation of endogenous ROS...
  21. doi request reprint Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast
    An M Aerts
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    FEBS Lett 583:113-7. 2009
    ..All these data confirm the importance of functional mitochondria and respiration in determining yeast chronological lifespan and apoptosis...
  22. ncbi request reprint Fungicidal activity of truncated analogues of dihydrosphingosine
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium
    Bioorg Med Chem Lett 18:3728-30. 2008
    ..e. C18-DHS). DHS derivatives that were active, that is, comprising 12, 15, 17, or 18 carbon atoms, induced accumulation of reactive oxygen species (ROS) in C. albicans...
  23. ncbi request reprint Heterologous production of human beta-defensin-2 in Arabidopsis thaliana
    A Aerts
    Centre of Microbial and Plant Genetics, Heverlee, Belgium
    Commun Agric Appl Biol Sci 70:51-5. 2005
  24. ncbi request reprint Isolation and characterization of Neurospora crassa mutants resistant to antifungal plant defensins
    Kathelijne K A Ferket
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Fungal Genet Biol 40:176-85. 2003
    ..Evidence is provided to link these clear differences in sphingolipid profiles of N. crassa mutants with their resistance towards different plant defensins...
  25. ncbi request reprint Therapeutic potential of antifungal plant and insect defensins
    Karin Thevissen
    Centre of Microbial and Plant Genetics, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Drug Discov Today 12:966-71. 2007
    ..Studies evaluating their in vivo antifungal efficacy demonstrate their therapeutic potential...
  26. ncbi request reprint Interactions of antifungal plant defensins with fungal membrane components
    Karin Thevissen
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Peptides 24:1705-12. 2003
    ..This review will focus on different classes of complex lipids in fungal membranes and on the selective interaction of plant defensins with these complex lipids...
  27. ncbi request reprint Level of M(IP)2C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast
    An M Aerts
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    FEBS Lett 580:1903-7. 2006
    ..In addition, DmTn11 and HsTnII are characterized by increased and reduced oxidative stress resistance/chronological life-span (CL), respectively. A putative involvement of M(IP)2C in oxidative stress and CL in yeast is discussed...
  28. ncbi request reprint Defensins from insects and plants interact with fungal glucosylceramides
    Karin Thevissen
    Centre of Microbial and Plant Genetics, Kasteelpark Arenberg 20, Heverlee B 3001, Belgium
    J Biol Chem 279:3900-5. 2004
    ....
  29. doi request reprint The antifungal plant defensin RsAFP2 from radish induces apoptosis in a metacaspase independent way in Candida albicans
    An M Aerts
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Heverlee, Belgium
    FEBS Lett 583:2513-6. 2009
    ..Moreover, our data point to the existence of at least two different types of caspases or caspase-like proteases in C. albicans...
  30. ncbi request reprint Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4
    Karin Thevissen
    Centre for Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    Peptides 26:1113-9. 2005
    ..Native Ib-AMPs showed no hemolytic nor toxic activity up to a concentration of 100 microM. All these data demonstrate the potential of the native Ib-AMPs to combat fungal infections...
  31. ncbi request reprint Plant defensins
    Bart P H J Thomma
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Heverlee Leuven, Belgium
    Planta 216:193-202. 2002
    ..This review focuses on the classification of plant defensins in general and in Arabidopsis specifically, and on the mode of action of plant defensins against fungal pathogens...
  32. ncbi request reprint Mode of action of plant defensins suggests therapeutic potential
    Bart P H J Thomma
    Centre of Microbial and Plant Genetics CMPG, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee Leuven, Belgium
    Curr Drug Targets Infect Disord 3:1-8. 2003
    ..Some of these defensins have been found to interact with fungal-specific components in the plasmamembrane, resulting in membrane permeabilization. This makes them an attractive source of potential therapeutics to treat fungal infections...
  33. ncbi request reprint Miconazole induces changes in actin cytoskeleton prior to reactive oxygen species induction in yeast
    Karin Thevissen
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium
    J Biol Chem 282:21592-7. 2007
    ..These data provide novel mechanistic insights in the mode of action of a ROS-inducing azole...
  34. ncbi request reprint The antifungal activity of RsAFP2, a plant defensin from raphanus sativus, involves the induction of reactive oxygen species in Candida albicans
    An M Aerts
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium
    J Mol Microbiol Biotechnol 13:243-7. 2007
    ..These data point to the presence of an intracellular plant defensin-induced signaling cascade, which involves ROS generation and leads to fungal cell growth arrest...
  35. ncbi request reprint Fungal infections and antifungal strategies
    Karin Thevissen
    Curr Drug Targets 6:847. 2005
  36. ncbi request reprint Antibacterial and antifungal properties of alpha-helical, cationic peptides in the venom of scorpions from southern Africa
    Leentje Moerman
    Interdisciplinary Research Center, Katholieke Universiteit Leuven Campus Kortrijk, Kortrijk, Belgium
    Eur J Biochem 269:4799-810. 2002
    ..Furthermore, all peptides inhibited growth of fungi. Experiments with SYTOX green suggested that this effect is related to membrane permeabilization...
  37. ncbi request reprint Neutral glycolipids of the filamentous fungus Neurospora crassa: altered expression in plant defensin-resistant mutants
    Chaeho Park
    Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 7229, USA
    J Lipid Res 46:759-68. 2005
    ..The potential implications of these findings with respect to defensin resistance in the N. crassa mutants are discussed...
  38. ncbi request reprint IPT1-independent sphingolipid biosynthesis and yeast inhibition by syringomycin E and plant defensin DmAMP1
    Yang Ju Im
    Department of Biology, Utah State University, Logan 84322 5305, USA
    FEMS Microbiol Lett 223:199-203. 2003
    ..These results show that the terminal sphingolipid but not IPT1 expression is required for fungicidal activity, and they suggest an IPT1-independent route for mannosyldiinositolphosphoceramide biosynthesis...