B Nilius

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Permeation and selectivity of TRP channels
    Grzegorz Owsianik
    Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Annu Rev Physiol 68:685-717. 2006
  2. ncbi request reprint What do we really know and what do we need to know: some controversies, perspectives, and surprises
    Bernd Nilius
    Department Cell Mol Medicine, Laboratory Ion Channel Research, KU Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49 bus 802, 3000, Leuven, Belgium
    Handb Exp Pharmacol 223:1239-80. 2014
  3. pmc TRPV3: time to decipher a poorly understood family member!
    Bernd Nilius
    Campus Gasthuisberg, Department of Physiology, KU Leuven, Campus Gasthuisberg, Herestraat 49, Leuven B 3000, Belgium
    J Physiol 592:295-304. 2014
  4. doi request reprint Spices: the savory and beneficial science of pungency
    Bernd Nilius
    KU Leuven Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research, Leuven, Belgium
    Rev Physiol Biochem Pharmacol 164:1-76. 2013
  5. pmc The puzzle of TRPV4 channelopathies
    Bernd Nilius
    KU Leuven, Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research, Campus Gasthuisberg, Leuven, Belgium
    EMBO Rep 14:152-63. 2013
  6. doi request reprint The transient receptor potential channel TRPA1: from gene to pathophysiology
    Bernd Nilius
    Department of Cellular and Molecular Medicine, Laboratory Ion Channel Research, KU Leuven, Campus Gasthuisberg, 3000, Leuven, Belgium
    Pflugers Arch 464:425-58. 2012
  7. pmc Immunohistochemistry using an antibody to unphosphorylated connexin 43 to identify human myometrial interstitial cells
    Graham Hutchings
    Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium
    Reprod Biol Endocrinol 6:43. 2008
  8. ncbi request reprint TRP channels in disease
    Bernd Nilius
    KU Leuven, Department of Molecular Cell Biology, Division of Physiology, Laboratory of Ion Channel Research, Campus Gasthuisberg, Herestraat 49, Bus 802, B 3000 Leuven, Belgium
    Biochim Biophys Acta 1772:805-12. 2007
  9. ncbi request reprint Transient receptor potential channelopathies
    Bernd Nilius
    Laboratory Ion Channel Research, Department of Molecular Cell Biology, KU Leuven, Campus Gasthuisberg, Herestraat 49, Bus 802, Leuven, Belgium
    Pflugers Arch 460:437-50. 2010
  10. ncbi request reprint Transient receptor potential (TRP) cation channels: rewarding unique proteins
    Bernd Nilius
    KU Leuven, Department of Cell Biology, Division Physiology, Campus Gasthuisberg, Belgium
    Bull Mem Acad R Med Belg 162:244-53. 2007

Collaborators

Detail Information

Publications139 found, 100 shown here

  1. ncbi request reprint Permeation and selectivity of TRP channels
    Grzegorz Owsianik
    Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Annu Rev Physiol 68:685-717. 2006
    ..This review gives an overview of available experimental and theoretical data and discusses the functional impact of pore-structure modifications on TRP channel properties...
  2. ncbi request reprint What do we really know and what do we need to know: some controversies, perspectives, and surprises
    Bernd Nilius
    Department Cell Mol Medicine, Laboratory Ion Channel Research, KU Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49 bus 802, 3000, Leuven, Belgium
    Handb Exp Pharmacol 223:1239-80. 2014
    ..We will conclude that our knowledge on TRP channels is still at the very beginning of an exciting research journey. ..
  3. pmc TRPV3: time to decipher a poorly understood family member!
    Bernd Nilius
    Campus Gasthuisberg, Department of Physiology, KU Leuven, Campus Gasthuisberg, Herestraat 49, Leuven B 3000, Belgium
    J Physiol 592:295-304. 2014
    ..More recently, mutations in TRPV3 were linked with a rare genodermatosis known as the Olmsted syndrome. This review gives an overview on properties of TRPV3 and its functions in the skin and skin diseases. ..
  4. doi request reprint Spices: the savory and beneficial science of pungency
    Bernd Nilius
    KU Leuven Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research, Leuven, Belgium
    Rev Physiol Biochem Pharmacol 164:1-76. 2013
    ....
  5. pmc The puzzle of TRPV4 channelopathies
    Bernd Nilius
    KU Leuven, Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research, Campus Gasthuisberg, Leuven, Belgium
    EMBO Rep 14:152-63. 2013
    ..Putative mechanisms to explain the puzzle, and how mutations in the same region of the channel cause different diseases, are discussed and experimental approaches to tackle this surprising problem are suggested...
  6. doi request reprint The transient receptor potential channel TRPA1: from gene to pathophysiology
    Bernd Nilius
    Department of Cellular and Molecular Medicine, Laboratory Ion Channel Research, KU Leuven, Campus Gasthuisberg, 3000, Leuven, Belgium
    Pflugers Arch 464:425-58. 2012
    ..Moreover, its role in the (patho)physiology of nearly all organ systems is anticipated, and will be discussed along with the potential of TRPA1 as a drug target for the management of various pathological conditions...
  7. pmc Immunohistochemistry using an antibody to unphosphorylated connexin 43 to identify human myometrial interstitial cells
    Graham Hutchings
    Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium
    Reprod Biol Endocrinol 6:43. 2008
    ....
  8. ncbi request reprint TRP channels in disease
    Bernd Nilius
    KU Leuven, Department of Molecular Cell Biology, Division of Physiology, Laboratory of Ion Channel Research, Campus Gasthuisberg, Herestraat 49, Bus 802, B 3000 Leuven, Belgium
    Biochim Biophys Acta 1772:805-12. 2007
    ..Finally, the role of TRPs will be discussed in neuronal diseases and neurodegenerative disorders...
  9. ncbi request reprint Transient receptor potential channelopathies
    Bernd Nilius
    Laboratory Ion Channel Research, Department of Molecular Cell Biology, KU Leuven, Campus Gasthuisberg, Herestraat 49, Bus 802, Leuven, Belgium
    Pflugers Arch 460:437-50. 2010
    ..Based on available genetic indications, we will also describe several putative pathological conditions in which (mal)function of TRP channels could be anticipated...
  10. ncbi request reprint Transient receptor potential (TRP) cation channels: rewarding unique proteins
    Bernd Nilius
    KU Leuven, Department of Cell Biology, Division Physiology, Campus Gasthuisberg, Belgium
    Bull Mem Acad R Med Belg 162:244-53. 2007
    ..Finally, TRP channels will become important novel pharmacological targets for the treatment of human diseases and for modulation of sensory functions, e.g. the perception of flavor...
  11. ncbi request reprint A road map for TR(I)Ps
    Bernd Nilius
    Laboratory of Physiology, KU Leuven, B 3000 Leuven, Belgium
    Mol Cell 22:297-307. 2006
    ....
  12. pmc Irritating channels: the case of TRPA1
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium
    J Physiol 589:1543-9. 2011
    ..Finally, we assess some problems inherent to activation of TRPA1 by the reaction of electrophilic compounds with the nucleophilic thiol sink of N-terminal reactive cysteines...
  13. pmc Transient receptor potential channels meet phosphoinositides
    Bernd Nilius
    Department of Molecular Cell Biology, Laboratory of Ion Channel Research, Campus Gasthuisberg, KU Leuven, Belgium
    EMBO J 27:2809-16. 2008
    ..In this review, we summarize current knowledge about the mechanisms of interaction between TRP channels and PIPs, and discuss the possible functional implications of TRP-PIP interactions to human physiology and pathophysiology...
  14. doi request reprint A TRP channel-steroid marriage
    Bernd Nilius
    Department of Molecular Cell Biology, Laboratory of Ion Channel Research, KU Leuven, Campus Gasthuisberg, Herestraat 49, Bus 802, B 3000 Leuven, Belgium
    Nat Cell Biol 10:1383-4. 2008
    ..A surprising functional association between TRPM3, a mysterious member of the family of transient receptor potential (TRP) cation channels, and the sulphated version of pregnenolone, 'mother' of all steroid hormones, has been identified...
  15. ncbi request reprint Polycystins under pressure
    Bernd Nilius
    KU Leuven, Department of Molecular Cell Biology, Laboratory Ion Channel Research, B 3000 Leuven, Belgium
    Cell 139:466-7. 2009
    ..In this issue of Cell, Sharif-Naeini et al. (2009) show that a candidate mechanosensing channel, TRPP2 (PKD2), unexpectedly acts as an inhibitor of a still-elusive stretch-activated cation channel in vascular smooth muscle cells...
  16. ncbi request reprint Transient receptor potential cation channels in disease
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KULeuven, Leuven, Belgium
    Physiol Rev 87:165-217. 2007
    ..In this review, we provide an overview of the impact of TRP channels on the pathogenesis of several diseases and identify several TRPs for which a causal pathogenic role might be anticipated...
  17. ncbi request reprint Regulation of TRP channels: a voltage-lipid connection
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem Soc Trans 35:105-8. 2007
    ..A PIP(2)-binding pocket seems to comprise a part of the TRP domain and especially pleckstrin homology domains in the C-terminus...
  18. ncbi request reprint From cardiac cation channels to the molecular dissection of the transient receptor potential channel TRPM4
    Bernd Nilius
    Laboratorium voor Fysiologie, Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Pflugers Arch 453:313-21. 2006
    ..A functional hallmark of TRPM4, as for several TRP channels, is a dramatic shift of its voltage dependence towards negative, physiologically meaningful potentials...
  19. ncbi request reprint T-type calcium channels: the never ending story
    Bernd Nilius
    Laboratorium voor Fysiologie, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Cell Calcium 40:81-8. 2006
    ..Finally, an overview is given how research on T-type Ca2+ channels has developed in the last years and in which topics it is branching out, a process, which is still ongoing...
  20. pmc Calcium-activated chloride channels in bovine pulmonary artery endothelial cells
    B Nilius
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Belgium
    J Physiol 498:381-96. 1997
    ..8. It is concluded that [Ca2+]i activates small-conductance Cl- channels in endothelial cells, which coexist with the volume-activated Cl- channels described previously...
  21. pmc Activation of volume-regulated chloride currents by reduction of intracellular ionic strength in bovine endothelial cells
    B Nilius
    Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium
    J Physiol 506:353-61. 1998
    ..7. It is concluded that a reduction of gamma 1 activates an anion current that is identical to that activated by cell swelling. Changes in intracellular ionic strength may shift the volume set point for activation of ICl,swell...
  22. pmc Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC
    B Nilius
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit, Leuven, Leuven, Belgium
    J Physiol 527:239-48. 2000
    ..It is concluded that the permeation profile of ECaC for monovalent cations suggests a strong field-strength binding site that may be involved in Ca2+ permeation and Mg2+ block...
  23. pmc The amino side of the C-terminus determines fast inactivation of the T-type calcium channel alpha1G
    M Staes
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B-3000 Leuven, Belgium
    J Physiol 530:35-45. 2001
    ....
  24. ncbi request reprint Inhibition of volume-regulated anion channels by dominant-negative caveolin-1
    D Trouet
    Laboratory of Physiology, Catholic University of Leuven, Campus Gasthuisberg, Leuven, B-3000, Belgium
    Biochem Biophys Res Commun 284:461-5. 2001
    ..We therefore conclude that the interaction between caveolin-1 and detergent-resistant lipid rafts is an important prerequisite for endothelial VRAC activity...
  25. pmc Role of Rho and Rho kinase in the activation of volume-regulated anion channels in bovine endothelial cells
    B Nilius
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    J Physiol 516:67-74. 1999
    ..9. These experiments provide strong evidence that the Rho-Rho kinase pathway is involved in the VRAC activation cascade...
  26. ncbi request reprint The C-terminal part of the R-domain, but not the PDZ binding motif, of CFTR is involved in interaction with Ca(2+)-activated Cl- channels
    L Wei
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 442:280-5. 2001
    ..It is concluded that the C-terminal part of the R-domain is an important determinant for CFTR-CaCC interaction...
  27. ncbi request reprint Modulation of volume-regulated anion channels by extra- and intracellular pH
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Pflugers Arch 436:742-8. 1998
    ..HCO3- and lactate permeation, as well as the up-regulation of VRAC at an acidic intracellular pH might be related to a possible role of this channel in cellular pH regulation...
  28. ncbi request reprint Mibefradil (Ro 40-5967) blocks multiple types of voltage-gated calcium channels in cultured rat spinal motoneurones
    F Viana
    Laboratory of Physiology, Catholic University of Leuven, Belgium
    Cell Calcium 22:299-311. 1997
    ..We conclude that mibefradil has potent blocking effects on Ca2+ channels in mammalian motoneurones. We hypothesize that therapeutic and pharmacological effects of mibefradil may involve actions on Ca2+ channels other than type T...
  29. ncbi request reprint Multiple types of chloride channels in bovine pulmonary artery endothelial cells
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, Belgium
    J Vasc Res 34:220-8. 1997
    ..We conclude that CPAE cells do not express voltage-gated (ClC-type) or cAMP-gated (CFTR-type) Cl- channels, but activate large Cl- currents after volume (mechanical?) or chemical (Ca2+) stimulation...
  30. ncbi request reprint Modulation of the epithelial calcium channel, ECaC, by intracellular Ca2+
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Calcium 29:417-28. 2001
    ..Slow recovery seems to depend on dissociation of Ca( 2+ from an internal Ca2+ binding site at ECaC...
  31. ncbi request reprint Kinetic and pharmacological properties of the calcium-activated chloride-current in macrovascular endothelial cells
    B Nilius
    Laboratorium voor Fysiologie, KU Leuven, Belgium
    Cell Calcium 22:53-63. 1997
    ..RT-PCR experiments did not provide any evidence that the endothelial Ca(2+)-activated Cl- channel might be identical with a recently cloned Ca(2+)-sensitive Cl- channel (CaCC)...
  32. pmc Sulphonic acid derivatives as probes of pore properties of volume-regulated anion channels in endothelial cells
    G Droogmans
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Br J Pharmacol 128:35-40. 1999
    ..From the structural information of the calixarenes, we estimate a lower and upper limit of 11*12 and 17*12 A2 respectively for the cross-sectional area of the pore...
  33. ncbi request reprint Effect of prolonged c-kit receptor inhibition by imatinib mesylate on the uterine contractility of pregnant rabbits
    G Hutchings
    Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium
    Gynecol Obstet Invest 65:108-11. 2008
    ..The c-kit receptor expressed by interstitial cells in the gastrointestinal tract is crucial to their pacemaking function. The function of similar c-kit-expressing myometrial cells is unknown...
  34. ncbi request reprint Annexin II modulates volume-activated chloride currents in vascular endothelial cells
    B Nilius
    Laboratorium voor Fysiologie, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 271:30631-6. 1996
    ..We conclude that annexin II-p11 complex formation is either directly or indirectly involved in the activation of ICl, vol in endothelial cells...
  35. ncbi request reprint Heat-evoked activation of TRPV4 channels in a HEK293 cell expression system and in native mouse aorta endothelial cells
    Hiroyuki Watanabe
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:47044-51. 2002
    ..The failure to activate TRPV4 channels by heat in inside-out patches, which responded to 4alphaPDD, may indicate that heat activation depends on the presence of an endogenous ligand, which is missing in inside-out patches...
  36. ncbi request reprint Amazing chloride channels: an overview
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, Leuven, Belgium
    Acta Physiol Scand 177:119-47. 2003
    ..Finally, we discuss the role of Cl- channels in various diseases and describe the pathological phenotypes observed in knockout mice models...
  37. ncbi request reprint Cellular function and control of volume-regulated anion channels
    J Eggermont
    Laboratory of Physiology, Catholic University of Leuven, Belgium
    Cell Biochem Biophys 35:263-74. 2001
    ..The second part concentrates on the Rho/Rho kinase/myosin phosphorylation cascade and on compartmentalization in caveolae as modulators of the signal transduction cascade that controls VRAC gating in vascular endothelial cells...
  38. ncbi request reprint Myosin light chain phosphorylation-dependent modulation of volume-regulated anion channels in macrovascular endothelium
    B Nilius
    Laboratorium voor Fysiologie, Campus Gasthuisberg, Leuven, Belgium
    FEBS Lett 466:346-50. 2000
    ..Conversely, NIPP1(191-210), an MLCP inhibitory peptide, potentiated the activation of VRAC by a 25% hypotonic stimulus. These data indicate that activation of VRAC is modulated by MLC phosphorylation...
  39. pmc Pore properties and ionic block of the rabbit epithelial calcium channel expressed in HEK 293 cells
    R Vennekens
    Department of Physiology, Campus Gasthuisberg, KULeuven, Leuven, Belgium
    J Physiol 530:183-91. 2001
    ....
  40. pmc Functional interaction between TRP4 and CFTR in mouse aorta endothelial cells
    L Wei
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    BMC Physiol 1:3. 2001
    ..This study describes the functional interaction between the putative Ca2+ channel TRP4 and the cystic fibrosis transmembrane conductance regulator, CFTR, in mouse aorta endothelium (MAEC)...
  41. ncbi request reprint Modulation of the epithelial Ca2+ channel ECaC by extracellular pH
    R Vennekens
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 442:237-42. 2001
    ..4 and 38 +/- 4 microM (n = 8) at pH 8.5. The affinity of ECaC for Ca2+ was also affected by extracellular pH, shifting from 4.8 +/- 0.7 microM (n = 6) at pH 6.0 to 161 +/- 30 nM (n = 5) at pH 7.4 and 425 +/- 117 nM (n = 8) at pH 8.5...
  42. ncbi request reprint Ion channels and their functional role in vascular endothelium
    B Nilius
    Department of Physiology, KU Leuven, Campus Gasthuisberg, Leuven, Belgium
    Physiol Rev 81:1415-59. 2001
    ....
  43. pmc Pharmacological modulation of monovalent cation currents through the epithelial Ca2+ channel ECaC1
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Belgium
    Br J Pharmacol 134:453-62. 2001
    ....
  44. ncbi request reprint Differential activation of the volume-sensitive cation channel TRP12 (OTRPC4) and volume-regulated anion currents in HEK-293 cells
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 443:227-33. 2001
    ..The kinetic and pharmacological profile of VRAC and TRP12 currents were also different...
  45. ncbi request reprint Current understanding of mammalian TRP homologues
    R Vennekens
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Calcium 31:253-64. 2002
    ..This review summarizes the currently available information concerning members of the TRP family expressed in mammalian tissues...
  46. ncbi request reprint CaT1 and the calcium release-activated calcium channel manifest distinct pore properties
    T Voets
    Laboratory of Physiology, Catholic University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 276:47767-70. 2001
    ..Our results imply that the pores of CaT1 and CRAC are not identical and indicate that CaT1 is a Mg(2+)-gated channel not directly related to CRAC...
  47. ncbi request reprint Functional characterization of the CFTR R domain using CFTR/MDR1 hybrid and deletion constructs
    A Vankeerberghen
    Center for Human Genetics and Laboratory of Physiology, University of Leuven, Gasthuisberg O and N6, Herestraat 49, B 3000 Leuven, Belgium
    Biochemistry 38:14988-98. 1999
    ..Taken together, these results suggest that the MDR1 linker domain can substitute for part of the regulatory domain of the CFTR protein...
  48. doi request reprint Modulation of the transient receptor potential channel TRPA1 by phosphatidylinositol 4,5-biphosphate manipulators
    Yuji Karashima
    KU Leuven Department of Mol Cell Biology, Lab Ion Channel Research, Campus Gasthuisberg, Herestraat 49, Bus 802, B 3000, Leuven, Belgium
    Pflugers Arch 457:77-89. 2008
    ....
  49. pmc TRPA1 acts as a cold sensor in vitro and in vivo
    Yuji Karashima
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg O and N1, Katholieke Universiteit Leuven, Herestraat 49 bus 802, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 106:1273-8. 2009
    ..Finally, cold plate and tail-flick experiments reveal TRPA1-dependent, cold-induced nociceptive behavior in mice. We conclude that TRPA1 acts as a major sensor for noxious cold...
  50. pmc Inhibition of volume-regulated anion channels in cultured endothelial cells by the anti-oestrogens clomiphene and nafoxidine
    C Maertens
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Br J Pharmacol 132:135-42. 2001
    ..The inhibition by clomiphene is not stereoselective and occurs at concentrations close to therapeutically relevant concentrations. Finally, both drugs inhibit the proliferation of endothelial cells...
  51. pmc Reduced intracellular ionic strength as the initial trigger for activation of endothelial volume-regulated anion channels
    T Voets
    Department of Physiology, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 96:5298-303. 1999
    ..Instead, they are in complete agreement with a model in which a decrease of Gammai rather than an increase in cell volume is the initial trigger for activation of ICl,swell...
  52. ncbi request reprint Calcium signalling through nucleotide receptor P2Y2 in cultured human vascular endothelium
    F Viana
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Belgium
    Cell Calcium 24:117-27. 1998
    ..These results indicate that P2Y2 receptors linked to intracellular Ca2+ mobilization are present in human vascular endothelial cells. The initial [Ca2+]i mobilization is followed by a phase of elevated [Ca2+]i influx...
  53. pmc Inhibition of volume-activated chloride currents in endothelial cells by chromones
    S Heinke
    KU Leuven, Laboratorium voor Fysiologie, Belgium
    Br J Pharmacol 115:1393-8. 1995
    ..6 It is concluded that blockade of volume-activated Cl- currents by extracellular sodium cromoglycatemay be due to an intracellular action following its permeation across the cell membrane...
  54. ncbi request reprint Structure-function relationship of the TRP channel superfamily
    G Owsianik
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Herestraat 49, 3000 Leuven, Belgium
    Rev Physiol Biochem Pharmacol 156:61-90. 2006
    ..This review summarizes and discusses the current knowledge on the TRP protein structure and its impact on the regulation of the channel function...
  55. pmc Cell swelling, heat, and chemical agonists use distinct pathways for the activation of the cation channel TRPV4
    J Vriens
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 101:396-401. 2004
    ..Phorbol esters and heat operate by means of a distinct, PLA(2)- and cytochrome p450 epoxygenase-independent pathway, which critically depends on an aromatic residue at the N terminus of the third transmembrane domain...
  56. ncbi request reprint Abnormal intracellular ca(2+)homeostasis and disease
    L Missiaen
    Laboratory of Physiology, K U Leuven Campus Gasthuisberg O N, Leuven, Belgium
    Cell Calcium 28:1-21. 2000
    ..In some instances, disease-causing mutations or abnormalities provide us with new insights into the cell biology of the various Ca(2+)transporters...
  57. ncbi request reprint The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven B 3000, Belgium
    J Biol Chem 276:1020-5. 2001
    ..Mutations of two neighboring negatively charged residues, i.e. Glu(535) and Asp(550), had only minor effects on Ca(2+) permeation properties...
  58. ncbi request reprint Inhibition of VRAC by c-Src tyrosine kinase targeted to caveolae is mediated by the Src homology domains
    D Trouet
    Laboratory of Physiology, Catholic University of Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium
    Am J Physiol Cell Physiol 281:C248-56. 2001
    ..We therefore conclude that c-Src-mediated inhibition of VRAC requires compartmentalization of c-Src to caveolae and that the Src homology domains 2 and/or 3 are necessary and sufficient for inhibition...
  59. ncbi request reprint Functional properties of the epithelial Ca2+ channel, ECaC
    R Vennekens
    Department of Physiology, KU Leuven, Belgium
    Gen Physiol Biophys 20:239-53. 2001
    ....
  60. ncbi request reprint Volume-activated Cl- channels
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Belgium
    Gen Pharmacol 27:1131-40. 1996
    ..4. Molecular candidates for this channel (i.e. ClC-2, a member of the ClC-family of voltage-dependent Cl- channels, the mdr-1 encoded P-glycoprotein, the nucleotide-sensitive pICln protein and phospholemman) will be discussed...
  61. ncbi request reprint Aspartate residues of the Glu-Glu-Asp-Asp (EEDD) pore locus control selectivity and permeation of the T-type Ca(2+) channel alpha(1G)
    K Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
    J Biol Chem 276:45628-35. 2001
    ..The mutation of both divergent aspartates only partially confers HVA channel permeation properties to the alpha(1G) Ca(2+) channel subunit...
  62. pmc Polyvariant mutant cystic fibrosis transmembrane conductance regulator genes. The polymorphic (Tg)m locus explains the partial penetrance of the T5 polymorphism as a disease mutation
    H Cuppens
    Center for Human Genetics, KULeuven, B 3000 Leuven, Belgium
    J Clin Invest 101:487-96. 1998
    ..Such polyvariant mutant genes could explain why apparently normal CFTR genes cause disease. Moreover, they might be responsible for variation in the phenotypic expression of CFTR mutations, and be of relevance in other genetic diseases...
  63. pmc Permeation properties and modulation of volume-activated Cl(-)-currents in human endothelial cells
    B Nilius
    K U Leuven, Laboratorium voor Fysiologie, Belgium
    Br J Pharmacol 112:1049-56. 1994
    ..Evidence that they belong to the class of P-glycoprotein associated Cl--channels is however only marginal, whereas their biophysical characteristics differ significantly from those of the CIC-2 volume-activated Cl--channels...
  64. ncbi request reprint The effect of imatinib mesylate on the contractility of isolated rabbit myometrial strips
    G Hutchings
    Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium
    Gynecol Obstet Invest 62:79-83. 2006
    ..C-kit receptor expressing interstitial cells generate and coordinate the electrical signals that control peristalsis in the gut. However, the function of interstitial cells in the myometrium is not known...
  65. ncbi request reprint The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels
    Thomas Voets
    Laboratory of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    Nature 430:748-54. 2004
    ..Our results suggest a simple unifying principle that explains both cold and heat sensitivity in TRP channels...
  66. ncbi request reprint Modulation of the Ca2 permeable cation channel TRPV4 by cytochrome P450 epoxygenases in vascular endothelium
    J Vriens
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Belgium
    Circ Res 97:908-15. 2005
    ..Together, our data establish that CYP-derived EETs modulate the activity of TRPV4 channels in endothelial cells and shows the unraveling of novel modulatory pathways via CYP2C modulation and sEH inhibition...
  67. ncbi request reprint Ca(2+)-activated Cl- channels in Ehrlich ascites tumor cells are distinct from mCLCA1, 2 and 3
    J Papassotiriou
    Laboratorium voor Fysiologie, KU Leuven, Campus Gasthuisberg, 3000 Leuven, Belgium
    Pflugers Arch 442:273-9. 2001
    ..Thus, phenotypic differences in combination with RT-PCR data point to the existence of different molecular species for Ca(2+)-activated Cl- channels...
  68. ncbi request reprint Block of volume-regulated anion channels by selective serotonin reuptake inhibitors
    C Maertens
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
    Naunyn Schmiedebergs Arch Pharmacol 366:158-65. 2002
    ..We conclude that VRAC block is a general property of this pharmacological class of selective serotonin reuptake inhibitors...
  69. pmc The Ca2+-activated cation channel TRPM4 is regulated by phosphatidylinositol 4,5-biphosphate
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    EMBO J 25:467-78. 2006
    ..Our data demonstrate that PIP2 is a strong positive modulator of TRPM4, and implicate the C-terminal PH domain in PIP2 action. PLC-mediated PIP2 breakdown may constitute a physiologically important brake on TRPM4 activity...
  70. ncbi request reprint Comparison of functional properties of the Ca2+-activated cation channels TRPM4 and TRPM5 from mice
    Nina D Ullrich
    Laboratorium voor Fysiologie, Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Cell Calcium 37:267-78. 2005
    ..TRPM4 displayed a 10-fold higher affinity for block by flufenamic acid. Importantly, ATP4- blocked TRPM4 with high affinity (IC50 of 0.8+/-0.1 microM), whereas TRPM5 is insensitive to ATP4- at concentrations up to 1 mM...
  71. ncbi request reprint Transient receptor potential channels in sensory neurons are targets of the antimycotic agent clotrimazole
    Victor Meseguer
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg O and N1, KU Leuven, B 3000 Leuven, Belgium
    J Neurosci 28:576-86. 2008
    ..Together, our results identify TRP channels in sensory neurons as molecular targets of CLT, and offer means to develop novel CLT preparations with fewer unwanted sensory side effects...
  72. ncbi request reprint Voltage dependence of the Ca2+-activated cation channel TRPM4
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven B 3000, Belgium
    J Biol Chem 278:30813-20. 2003
    ..Voltage dependence of TRPM4 may be functionally important, especially in excitable tissues generating plateau-like or bursting action potentials...
  73. pmc Mg2+-dependent gating and strong inward rectification of the cation channel TRPV6
    Thomas Voets
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Belgium
    J Gen Physiol 121:245-60. 2003
    ..The effects of intracellular Mg(2+) on TRPV6 are partially reminiscent of the gating mechanism of inwardly rectifying K(+) channels and may represent a novel regulatory mechanism for TRPV6 function in vivo...
  74. ncbi request reprint Activation of TRPV4 channels (hVRL-2/mTRP12) by phorbol derivatives
    Hiroyuki Watanabe
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:13569-77. 2002
    ..We concluded that the phorbol derivatives activate TRPV4 (VR-OAC, VRL-2, OTRPC4, TRP12) independently from protein kinase C, in a manner consistent with direct agonist gating of the channel...
  75. pmc Extracellular Ca2+ modulates the effects of protons on gating and conduction properties of the T-type Ca2+ channel alpha1G (CaV3.1)
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Belgium
    J Gen Physiol 121:511-28. 2003
    ....
  76. pmc Pore structure influences gating properties of the T-type Ca2+ channel alpha1G
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Belgium
    J Gen Physiol 121:529-40. 2003
    ..These changes are independent of the alterations of the selectivity properties and channel protonation...
  77. ncbi request reprint Bimodal action of menthol on the transient receptor potential channel TRPA1
    Yuji Karashima
    Laboratorium voor Fysiologie, Campus Gasthuisberg, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Neurosci 27:9874-84. 2007
    ....
  78. doi request reprint The calcium-conducting ion channel transient receptor potential canonical 6 is involved in macrophage inflammatory protein-2-induced migration of mouse neutrophils
    N Damann
    Department of Molecular Cell Biology, Laboratory of Ion Channel Research, KU Leuven, Leuven, Belgium
    Acta Physiol (Oxf) 195:3-11. 2009
    ..The role of the calcium-conducting ion channel transient receptor potential canonical 6 (TRPC6) in macrophage inflammatory protein-2 (MIP-2) induced migration of mouse neutrophils was investigated...
  79. ncbi request reprint The endothelial volume-regulated anion channel, VRAC
    B Nilius
    Laboratory of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Physiol Biochem 10:313-20. 2000
    ..The functional impact of VRAC as a possible mechano-sensor in endothelium and as a regulatory component in the cell cycle, endothelial cell proliferation and possible angiogenesis will be discussed...
  80. ncbi request reprint Increased IgE-dependent mast cell activation and anaphylactic responses in mice lacking the calcium-activated nonselective cation channel TRPM4
    Rudi Vennekens
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg, O and N1, KU Leuven, Herestraat 49 bus 802, B 3000 Leuven, Belgium
    Nat Immunol 8:312-20. 2007
    ..Our results establish the physiological function of TRPM4 channels as critical regulators of Ca(2+) entry in mast cells...
  81. pmc Deletion of the transient receptor potential cation channel TRPV4 impairs murine bladder voiding
    Thomas Gevaert
    Department of Urology, University Hospital Gasthuisberg, Leuven, Belgium
    J Clin Invest 117:3453-62. 2007
    ..These data demonstrate a previously unrecognized role for TRPV4 in voiding behavior, raising the possibility that TRPV4 plays a critical role in urothelium-mediated transduction of intravesical mechanical pressure...
  82. ncbi request reprint Stimulus-specific modulation of the cation channel TRPV4 by PACSIN 3
    Dieter D'Hoedt
    Department of Physiology, Katholieke Universiteit Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49 bus 802, Leuven, Belgium
    J Biol Chem 283:6272-80. 2008
    ..Taken together, these data suggest that PACSIN 3 acts as an auxiliary protein of TRPV4 channel that not only affects the channel's subcellular localization but also modulates its function in a stimulus-specific manner...
  83. ncbi request reprint Evidence for common structural determinants of activation and inactivation in T-type Ca2+ channels
    Karel Talavera
    Laboratorium voor Fysiologie, KU Leuven, Campus Gasthuisberg, Leuven, Belgium
    Pflugers Arch 453:189-201. 2006
    ..1. Our results indicate that common structures, most likely the S6 transmembrane segments, are involved in the conformational changes occurring during both channel activation and inactivation...
  84. ncbi request reprint TRPM8-independent menthol-induced Ca2+ release from endoplasmic reticulum and Golgi
    Frank Mahieu
    Department of Molecular Cell Biology, Division of Physiology, Laboratory of Ion Channel Research, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 282:3325-36. 2007
    ..Finally, aequorin-based measurements demonstrate that the TRPM8-independent menthol-induced Ca2+ release originates from both endoplasmic reticulum and Golgi compartments...
  85. ncbi request reprint The TRPV4 channel: structure-function relationship and promiscuous gating behaviour
    Bernd Nilius
    Department of Physiology, KU Leuven, Campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 446:298-303. 2003
    ..Possible functional roles of this channel will be correlated with its observed expression pattern. Finally, we discuss the structural determinants of TRPV4 channel function...
  86. ncbi request reprint Determinants of 4 alpha-phorbol sensitivity in transmembrane domains 3 and 4 of the cation channel TRPV4
    Joris Vriens
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 282:12796-803. 2007
    ....
  87. ncbi request reprint The taste transduction channel TRPM5 is a locus for bitter-sweet taste interactions
    Karel Talavera
    Laboratory of Ion Channel Research, Dept of Molecular Cell Biology, Herestraat 49, Campus Gasthuisberg, O and N1, KU Leuven, B 3000 Leuven, Belgium
    FASEB J 22:1343-55. 2008
    ..The inhibition of TRPM5 by bitter compounds constitutes the molecular basis of a novel mechanism of taste interactions, whereby the bitter tastant inhibits directly the sweet transduction pathway...
  88. ncbi request reprint TRPM8 voltage sensor mutants reveal a mechanism for integrating thermal and chemical stimuli
    Thomas Voets
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg O and N1, KU Leuven, Herestraat 49 bus 802, B 3000 Leuven, Belgium
    Nat Chem Biol 3:174-82. 2007
    ..Based on these findings, we present a Monod-Wyman-Changeux-type model explaining the combined effects of voltage, temperature and menthol on TRPM8 gating...
  89. pmc Modulation of TRPs by PIPs
    Thomas Voets
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, KU Leuven, Onderwijs and Navorsing 1, Herestraat 49 bus 802, 3000 Leuven, Belgium
    J Physiol 582:939-44. 2007
    ....
  90. ncbi request reprint Transient receptor potential channels in endothelium: solving the calcium entry puzzle?
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg O and N, KU Leuven, Leuven, Belgium
    Endothelium 10:5-15. 2003
    ....
  91. ncbi request reprint TRP channels in human prostate
    Carl Van Haute
    Department of Molecular Cell Biology, Laboratory of Ion Channel Research, Campus Gasthuisberg, KULeuven, Belgium
    ScientificWorldJournal 10:1597-611. 2010
    ..Controversial data regarding the expression pattern and the potential impact of TRP channels in prostate function, and their involvement in prostate cancer and other prostate diseases, will be discussed...
  92. ncbi request reprint Invertebrate TRP proteins as functional models for mammalian channels
    Joris Vriens
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 449:213-26. 2004
    ....
  93. ncbi request reprint The selectivity filter of the cation channel TRPM4
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Belgium
    J Biol Chem 280:22899-906. 2005
    ..Our findings delineate the selectivity filter of TRPM channels and provide the first insight into the molecular basis of monovalent cation selectivity...
  94. pmc Functional characterization of transient receptor potential channels in mouse urothelial cells
    Wouter Everaerts
    Department of Molecular Cell Biology, Laboratory Ion Channel Research, Campus Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium
    Am J Physiol Renal Physiol 298:F692-701. 2010
    ..These channels may contribute to the (mechano)sensory function of the urothelial layer and represent potential targets for the treatment of bladder dysfunction...
  95. pmc Gating of TRP channels: a voltage connection?
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    J Physiol 567:35-44. 2005
    ..Some structural considerations will be given indicating that, although the voltage sensor is not yet known, the C-terminus may substantially change the voltage dependence of these channels...
  96. pmc Modulation of the cold-activated cation channel TRPM8 by surface charge screening
    Frank Mahieu
    KU Leuven, Laboratory of Ion Channel Research, Department of Molecular Cell Biology, Leuven, Belgium
    J Physiol 588:315-24. 2010
    ..These results represent the first description of the effects of surface charge screening on a TRP channel and provide a straightforward explanation for the known effects of extracellular Ca(2+) on cold-sensitive neurons...
  97. doi request reprint Nicotine activates the chemosensory cation channel TRPA1
    Karel Talavera
    Laboratory for Ion Channel Research, Department of Molecular Cell Biology, KU Leuven, Leuven, Belgium
    Nat Neurosci 12:1293-9. 2009
    ..The identification of TRPA1 as a nicotine target suggests that existing models of nicotine-induced irritation should be revised and may facilitate the development of smoking cessation therapies with less adverse effects...
  98. ncbi request reprint Heat activation of TRPM5 underlies thermal sensitivity of sweet taste
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    Nature 438:1022-5. 2005
    ....
  99. ncbi request reprint Where is TRPV1 expressed in the bladder, do we see the real channel?
    Wouter Everaerts
    Department of Surgery, Laboratory of Experimental Urology, Campus Gasthuisberg, KU Leuven, Herestraat 49, Bus 7003, 3000, Leuven, Belgium
    Naunyn Schmiedebergs Arch Pharmacol 379:421-5. 2009
    ..In conclusion, we think that the use of negative controls on knockout mice, whenever available, is mandatory when conducting immunohistochemical localization studies...
  100. ncbi request reprint TRPM4 regulates migration of mast cells in mice
    Takahiro Shimizu
    Department of Molecular Cell Biology, Laboratory of Ion Channel Research, KU Leuven, Campus Gasthuisberg, Herestraat 49, Bus 802, B 3000 Leuven, Belgium
    Cell Calcium 45:226-32. 2009
    ..Thus, our results suggest that TRPM4 is critically involved in migration of BMMCs by regulation of Ca(2+)-dependent actin cytoskeleton rearrangements...
  101. ncbi request reprint Diversity of TRP channel activation
    Bernd Nilius
    KU Leuven, Department of Physiology, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Novartis Found Symp 258:140-9; discussion 149-59, 263-6. 2004
    ..Regulation of channel availability by interaction with a protein bound to a site at the C-terminus of both channels will be demonstrated. Functional consequences of these different mechanisms of gating will be discussed...