B Nilius

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Heat-evoked activation of TRPV4 channels in a HEK293 cell expression system and in native mouse aorta endothelial cells
    Hiroyuki Watanabe
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:47044-51. 2002
  2. ncbi request reprint Differential activation of the volume-sensitive cation channel TRP12 (OTRPC4) and volume-regulated anion currents in HEK-293 cells
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 443:227-33. 2001
  3. ncbi request reprint TRPV4 calcium entry channel: a paradigm for gating diversity
    Bernd Nilius
    Laboratorium voor Fysiologie, KU Leuven, Campus Gasthuisberg, 3000 Leuven, Belgium
    Am J Physiol Cell Physiol 286:C195-205. 2004
  4. ncbi request reprint Myosin light chain phosphorylation-dependent modulation of volume-regulated anion channels in macrovascular endothelium
    B Nilius
    Laboratorium voor Fysiologie, Campus Gasthuisberg, Leuven, Belgium
    FEBS Lett 466:346-50. 2000
  5. pmc The Ca2+-activated cation channel TRPM4 is regulated by phosphatidylinositol 4,5-biphosphate
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    EMBO J 25:467-78. 2006
  6. pmc Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC
    B Nilius
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit, Leuven, Leuven, Belgium
    J Physiol 527:239-48. 2000
  7. ncbi request reprint The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven B 3000, Belgium
    J Biol Chem 276:1020-5. 2001
  8. ncbi request reprint The endothelial volume-regulated anion channel, VRAC
    B Nilius
    Laboratory of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Physiol Biochem 10:313-20. 2000
  9. ncbi request reprint Pflügers Archiv and the advent of modern electrophysiology. From the first action potential to patch clamp
    Bernd Nilius
    Laboratorium voor Fysiologie, KU Leuven, Campus Gasthuisberg, 3000, Leuven, Belgium
    Pflugers Arch 447:267-71. 2003
  10. ncbi request reprint Modulation of the epithelial calcium channel, ECaC, by intracellular Ca2+
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Calcium 29:417-28. 2001

Collaborators

Detail Information

Publications127 found, 100 shown here

  1. ncbi request reprint Heat-evoked activation of TRPV4 channels in a HEK293 cell expression system and in native mouse aorta endothelial cells
    Hiroyuki Watanabe
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:47044-51. 2002
    ..The failure to activate TRPV4 channels by heat in inside-out patches, which responded to 4alphaPDD, may indicate that heat activation depends on the presence of an endogenous ligand, which is missing in inside-out patches...
  2. ncbi request reprint Differential activation of the volume-sensitive cation channel TRP12 (OTRPC4) and volume-regulated anion currents in HEK-293 cells
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 443:227-33. 2001
    ..The kinetic and pharmacological profile of VRAC and TRP12 currents were also different...
  3. ncbi request reprint TRPV4 calcium entry channel: a paradigm for gating diversity
    Bernd Nilius
    Laboratorium voor Fysiologie, KU Leuven, Campus Gasthuisberg, 3000 Leuven, Belgium
    Am J Physiol Cell Physiol 286:C195-205. 2004
    ..This review summarizes data on TRPV4 as a paradigm of gating diversity in this subfamily of Ca2+ entry channels...
  4. ncbi request reprint Myosin light chain phosphorylation-dependent modulation of volume-regulated anion channels in macrovascular endothelium
    B Nilius
    Laboratorium voor Fysiologie, Campus Gasthuisberg, Leuven, Belgium
    FEBS Lett 466:346-50. 2000
    ..Conversely, NIPP1(191-210), an MLCP inhibitory peptide, potentiated the activation of VRAC by a 25% hypotonic stimulus. These data indicate that activation of VRAC is modulated by MLC phosphorylation...
  5. pmc The Ca2+-activated cation channel TRPM4 is regulated by phosphatidylinositol 4,5-biphosphate
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    EMBO J 25:467-78. 2006
    ..Our data demonstrate that PIP2 is a strong positive modulator of TRPM4, and implicate the C-terminal PH domain in PIP2 action. PLC-mediated PIP2 breakdown may constitute a physiologically important brake on TRPM4 activity...
  6. pmc Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC
    B Nilius
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit, Leuven, Leuven, Belgium
    J Physiol 527:239-48. 2000
    ..It is concluded that the permeation profile of ECaC for monovalent cations suggests a strong field-strength binding site that may be involved in Ca2+ permeation and Mg2+ block...
  7. ncbi request reprint The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven B 3000, Belgium
    J Biol Chem 276:1020-5. 2001
    ..Mutations of two neighboring negatively charged residues, i.e. Glu(535) and Asp(550), had only minor effects on Ca(2+) permeation properties...
  8. ncbi request reprint The endothelial volume-regulated anion channel, VRAC
    B Nilius
    Laboratory of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Physiol Biochem 10:313-20. 2000
    ..The functional impact of VRAC as a possible mechano-sensor in endothelium and as a regulatory component in the cell cycle, endothelial cell proliferation and possible angiogenesis will be discussed...
  9. ncbi request reprint Pflügers Archiv and the advent of modern electrophysiology. From the first action potential to patch clamp
    Bernd Nilius
    Laboratorium voor Fysiologie, KU Leuven, Campus Gasthuisberg, 3000, Leuven, Belgium
    Pflugers Arch 447:267-71. 2003
    ..Bernstein, the first detailed description of the conductance properties of excitable tissues by L. Hermann, and more recently the publication of the patch-clamp method by E. Neher and B. Sakmann...
  10. ncbi request reprint Modulation of the epithelial calcium channel, ECaC, by intracellular Ca2+
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Calcium 29:417-28. 2001
    ..Slow recovery seems to depend on dissociation of Ca( 2+ from an internal Ca2+ binding site at ECaC...
  11. ncbi request reprint Voltage dependence of the Ca2+-activated cation channel TRPM4
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven B 3000, Belgium
    J Biol Chem 278:30813-20. 2003
    ..Voltage dependence of TRPM4 may be functionally important, especially in excitable tissues generating plateau-like or bursting action potentials...
  12. ncbi request reprint The carboxyl terminus of the epithelial Ca(2+) channel ECaC1 is involved in Ca(2+)-dependent inactivation
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 445:584-8. 2003
    ..In conclusion, we have identified two domains in the carboxyl terminus of ECaC1 that control Ca(2+)-dependent inactivation...
  13. ncbi request reprint The TRPV4 channel: structure-function relationship and promiscuous gating behaviour
    Bernd Nilius
    Department of Physiology, KU Leuven, Campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 446:298-303. 2003
    ..Possible functional roles of this channel will be correlated with its observed expression pattern. Finally, we discuss the structural determinants of TRPV4 channel function...
  14. ncbi request reprint Transient receptor potential channels in endothelium: solving the calcium entry puzzle?
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg O and N, KU Leuven, Leuven, Belgium
    Endothelium 10:5-15. 2003
    ....
  15. ncbi request reprint Fast and slow inactivation kinetics of the Ca2+ channels ECaC1 and ECaC2 (TRPV5 and TRPV6). Role of the intracellular loop located between transmembrane segments 2 and 3
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, Herestraat 49, B 3000 KU Leuven, Belgium
    J Biol Chem 277:30852-8. 2002
    ..In conclusion, the TM2-TM3 loop is a critical determinant of the inactivation in ECaC1 and ECaC2...
  16. ncbi request reprint Ion channels and their functional role in vascular endothelium
    B Nilius
    Department of Physiology, KU Leuven, Campus Gasthuisberg, Leuven, Belgium
    Physiol Rev 81:1415-59. 2001
    ....
  17. pmc Calcium-impermeable monovalent cation channels: a TRP connection?
    Bernd Nilius
    KU Leuven, Campus Gasthuisberg, Department of Physiology, Herestraat 49, B 3000 Leuven, Belgium
    Br J Pharmacol 138:5-7. 2003
  18. ncbi request reprint Intracellular nucleotides and polyamines inhibit the Ca2+-activated cation channel TRPM4b
    Bernd Nilius
    Laboratorium voor Fysiologie Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 448:70-5. 2004
    ..Spermine blocked TRPM4 currents with an IC(50) of 61 microM. In conclusion, TRPM4 is a channel that can be effectively modulated by intracellular nucleotides and polyamines...
  19. pmc Role of Rho and Rho kinase in the activation of volume-regulated anion channels in bovine endothelial cells
    B Nilius
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    J Physiol 516:67-74. 1999
    ..9. These experiments provide strong evidence that the Rho-Rho kinase pathway is involved in the VRAC activation cascade...
  20. ncbi request reprint TRP channels: a TR(I)P through a world of multifunctional cation channels
    Bernd Nilius
    Laboratorium voor Fysiologie, Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven 3000, Belgium
    Pflugers Arch 451:1-10. 2005
    ..Hopefully, a better understanding of TRP channel physiology will provide important insight into the relationship between TRP channel dysfunction and human diseases...
  21. pmc Calcium-activated chloride channels in bovine pulmonary artery endothelial cells
    B Nilius
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Belgium
    J Physiol 498:381-96. 1997
    ..8. It is concluded that [Ca2+]i activates small-conductance Cl- channels in endothelial cells, which coexist with the volume-activated Cl- channels described previously...
  22. ncbi request reprint Volume-activated Cl- channels
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Belgium
    Gen Pharmacol 27:1131-40. 1996
    ..4. Molecular candidates for this channel (i.e. ClC-2, a member of the ClC-family of voltage-dependent Cl- channels, the mdr-1 encoded P-glycoprotein, the nucleotide-sensitive pICln protein and phospholemman) will be discussed...
  23. ncbi request reprint Annexin II modulates volume-activated chloride currents in vascular endothelial cells
    B Nilius
    Laboratorium voor Fysiologie, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 271:30631-6. 1996
    ..We conclude that annexin II-p11 complex formation is either directly or indirectly involved in the activation of ICl, vol in endothelial cells...
  24. pmc Gating of TRP channels: a voltage connection?
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    J Physiol 567:35-44. 2005
    ..Some structural considerations will be given indicating that, although the voltage sensor is not yet known, the C-terminus may substantially change the voltage dependence of these channels...
  25. pmc Decavanadate modulates gating of TRPM4 cation channels
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    J Physiol 560:753-65. 2004
    ..These results indicate that the C-terminus of TRPM4 is critically involved in mediating the DV effects. In conclusion, decavanadate modulates TRPM4, but not TRPM5, by inhibiting voltage-dependent closure of the channel...
  26. ncbi request reprint Regulation of the Ca2+ sensitivity of the nonselective cation channel TRPM4
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 280:6423-33. 2005
    ..Thus, the Ca(2+) sensitivity of TRPM4 is regulated by ATP, PKC-dependent phosphorylation, and calmodulin binding at the C terminus...
  27. ncbi request reprint Store-operated Ca2+ entry channels: still elusive!
    Bernd Nilius
    Department of Physiology, KU Leuven, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Sci STKE 2004:pe36. 2004
    ..This perspective summarizes problems in identifying SOCs, suggests approaches to solving these problems, and discusses concerns over the current tendency to focus exclusively on the hypothesis that TRPCs comprise SOCs...
  28. ncbi request reprint Multiple types of chloride channels in bovine pulmonary artery endothelial cells
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, Belgium
    J Vasc Res 34:220-8. 1997
    ..We conclude that CPAE cells do not express voltage-gated (ClC-type) or cAMP-gated (CFTR-type) Cl- channels, but activate large Cl- currents after volume (mechanical?) or chemical (Ca2+) stimulation...
  29. ncbi request reprint Kinetic and pharmacological properties of the calcium-activated chloride-current in macrovascular endothelial cells
    B Nilius
    Laboratorium voor Fysiologie, KU Leuven, Belgium
    Cell Calcium 22:53-63. 1997
    ..RT-PCR experiments did not provide any evidence that the endothelial Ca(2+)-activated Cl- channel might be identical with a recently cloned Ca(2+)-sensitive Cl- channel (CaCC)...
  30. ncbi request reprint Modulation of volume-regulated anion channels by extra- and intracellular pH
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Pflugers Arch 436:742-8. 1998
    ..HCO3- and lactate permeation, as well as the up-regulation of VRAC at an acidic intracellular pH might be related to a possible role of this channel in cellular pH regulation...
  31. ncbi request reprint Is the volume-regulated anion channel VRAC a "water-permeable" channel?
    Bernd Nilius
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Neurochem Res 29:3-8. 2004
    ..It is concluded that water permeates VRAC and might be able to accelerate water transport by providing an additional permeation pathway for water. Therefore VRAC can be considered as a water-permeable, "wet" channel...
  32. ncbi request reprint The selectivity filter of the cation channel TRPM4
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Belgium
    J Biol Chem 280:22899-906. 2005
    ..Our findings delineate the selectivity filter of TRPM channels and provide the first insight into the molecular basis of monovalent cation selectivity...
  33. pmc Activation of volume-regulated chloride currents by reduction of intracellular ionic strength in bovine endothelial cells
    B Nilius
    Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium
    J Physiol 506:353-61. 1998
    ..7. It is concluded that a reduction of gamma 1 activates an anion current that is identical to that activated by cell swelling. Changes in intracellular ionic strength may shift the volume set point for activation of ICl,swell...
  34. ncbi request reprint TRP channels in disease
    Bernd Nilius
    Department of Physiology, Campus Gasthuisberg Katholieke Universiteit, Leuven, Belgium
    Sci STKE 2005:re8. 2005
    ..An understanding of the genetics of disease may lead to the development of targeted new therapies...
  35. pmc The amino side of the C-terminus determines fast inactivation of the T-type calcium channel alpha1G
    M Staes
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B-3000 Leuven, Belgium
    J Physiol 530:35-45. 2001
    ....
  36. ncbi request reprint Inhibition of volume-regulated anion channels by dominant-negative caveolin-1
    D Trouet
    Laboratory of Physiology, Catholic University of Leuven, Campus Gasthuisberg, Leuven, B-3000, Belgium
    Biochem Biophys Res Commun 284:461-5. 2001
    ..We therefore conclude that the interaction between caveolin-1 and detergent-resistant lipid rafts is an important prerequisite for endothelial VRAC activity...
  37. ncbi request reprint Amazing chloride channels: an overview
    B Nilius
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, Leuven, Belgium
    Acta Physiol Scand 177:119-47. 2003
    ..Finally, we discuss the role of Cl- channels in various diseases and describe the pathological phenotypes observed in knockout mice models...
  38. pmc Properties of heterologously expressed hTRP3 channels in bovine pulmonary artery endothelial cells
    M Kamouchi
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Physiol 518:345-58. 1999
    ..6. It is concluded that hTRP3 forms non-selective plasmalemmal cation channels that function as a pathway for agonist-induced Ca2+ influx...
  39. ncbi request reprint The C-terminal part of the R-domain, but not the PDZ binding motif, of CFTR is involved in interaction with Ca(2+)-activated Cl- channels
    L Wei
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 442:280-5. 2001
    ..It is concluded that the C-terminal part of the R-domain is an important determinant for CFTR-CaCC interaction...
  40. pmc Sulphonic acid derivatives as probes of pore properties of volume-regulated anion channels in endothelial cells
    G Droogmans
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Br J Pharmacol 128:35-40. 1999
    ..From the structural information of the calixarenes, we estimate a lower and upper limit of 11*12 and 17*12 A2 respectively for the cross-sectional area of the pore...
  41. pmc Pore properties and ionic block of the rabbit epithelial calcium channel expressed in HEK 293 cells
    R Vennekens
    Department of Physiology, Campus Gasthuisberg, KULeuven, Leuven, Belgium
    J Physiol 530:183-91. 2001
    ....
  42. ncbi request reprint Mibefradil (Ro 40-5967) blocks multiple types of voltage-gated calcium channels in cultured rat spinal motoneurones
    F Viana
    Laboratory of Physiology, Catholic University of Leuven, Belgium
    Cell Calcium 22:299-311. 1997
    ..We conclude that mibefradil has potent blocking effects on Ca2+ channels in mammalian motoneurones. We hypothesize that therapeutic and pharmacological effects of mibefradil may involve actions on Ca2+ channels other than type T...
  43. pmc Functional interaction between TRP4 and CFTR in mouse aorta endothelial cells
    L Wei
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    BMC Physiol 1:3. 2001
    ..This study describes the functional interaction between the putative Ca2+ channel TRP4 and the cystic fibrosis transmembrane conductance regulator, CFTR, in mouse aorta endothelium (MAEC)...
  44. pmc Cell swelling, heat, and chemical agonists use distinct pathways for the activation of the cation channel TRPV4
    J Vriens
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 101:396-401. 2004
    ..Phorbol esters and heat operate by means of a distinct, PLA(2)- and cytochrome p450 epoxygenase-independent pathway, which critically depends on an aromatic residue at the N terminus of the third transmembrane domain...
  45. ncbi request reprint Functional characterization of the CFTR R domain using CFTR/MDR1 hybrid and deletion constructs
    A Vankeerberghen
    Center for Human Genetics and Laboratory of Physiology, University of Leuven, Gasthuisberg O and N6, Herestraat 49, B 3000 Leuven, Belgium
    Biochemistry 38:14988-98. 1999
    ..Taken together, these results suggest that the MDR1 linker domain can substitute for part of the regulatory domain of the CFTR protein...
  46. ncbi request reprint Modulation of the epithelial Ca2+ channel ECaC by extracellular pH
    R Vennekens
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium
    Pflugers Arch 442:237-42. 2001
    ..4 and 38 +/- 4 microM (n = 8) at pH 8.5. The affinity of ECaC for Ca2+ was also affected by extracellular pH, shifting from 4.8 +/- 0.7 microM (n = 6) at pH 6.0 to 161 +/- 30 nM (n = 5) at pH 7.4 and 425 +/- 117 nM (n = 8) at pH 8.5...
  47. ncbi request reprint Inhibition of VRAC by c-Src tyrosine kinase targeted to caveolae is mediated by the Src homology domains
    D Trouet
    Laboratory of Physiology, Catholic University of Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium
    Am J Physiol Cell Physiol 281:C248-56. 2001
    ..We therefore conclude that c-Src-mediated inhibition of VRAC requires compartmentalization of c-Src to caveolae and that the Src homology domains 2 and/or 3 are necessary and sufficient for inhibition...
  48. ncbi request reprint Cellular function and control of volume-regulated anion channels
    J Eggermont
    Laboratory of Physiology, Catholic University of Leuven, Belgium
    Cell Biochem Biophys 35:263-74. 2001
    ..The second part concentrates on the Rho/Rho kinase/myosin phosphorylation cascade and on compartmentalization in caveolae as modulators of the signal transduction cascade that controls VRAC gating in vascular endothelial cells...
  49. pmc Pharmacological modulation of monovalent cation currents through the epithelial Ca2+ channel ECaC1
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Belgium
    Br J Pharmacol 134:453-62. 2001
    ....
  50. ncbi request reprint Effect of prolonged c-kit receptor inhibition by imatinib mesylate on the uterine contractility of pregnant rabbits
    G Hutchings
    Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium
    Gynecol Obstet Invest 65:108-11. 2008
    ..The c-kit receptor expressed by interstitial cells in the gastrointestinal tract is crucial to their pacemaking function. The function of similar c-kit-expressing myometrial cells is unknown...
  51. pmc Inhibition of volume-regulated anion channels in cultured endothelial cells by the anti-oestrogens clomiphene and nafoxidine
    C Maertens
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Br J Pharmacol 132:135-42. 2001
    ..The inhibition by clomiphene is not stereoselective and occurs at concentrations close to therapeutically relevant concentrations. Finally, both drugs inhibit the proliferation of endothelial cells...
  52. ncbi request reprint CaT1 and the calcium release-activated calcium channel manifest distinct pore properties
    T Voets
    Laboratory of Physiology, Catholic University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 276:47767-70. 2001
    ..Our results imply that the pores of CaT1 and CRAC are not identical and indicate that CaT1 is a Mg(2+)-gated channel not directly related to CRAC...
  53. pmc Inhibition of volume-activated chloride currents in endothelial cells by chromones
    S Heinke
    KU Leuven, Laboratorium voor Fysiologie, Belgium
    Br J Pharmacol 115:1393-8. 1995
    ..6 It is concluded that blockade of volume-activated Cl- currents by extracellular sodium cromoglycatemay be due to an intracellular action following its permeation across the cell membrane...
  54. ncbi request reprint Block of volume-regulated anion channels by selective serotonin reuptake inhibitors
    C Maertens
    KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
    Naunyn Schmiedebergs Arch Pharmacol 366:158-65. 2002
    ..We conclude that VRAC block is a general property of this pharmacological class of selective serotonin reuptake inhibitors...
  55. doi request reprint Nicotine activates the chemosensory cation channel TRPA1
    Karel Talavera
    Laboratory for Ion Channel Research, Department of Molecular Cell Biology, KU Leuven, Leuven, Belgium
    Nat Neurosci 12:1293-9. 2009
    ..The identification of TRPA1 as a nicotine target suggests that existing models of nicotine-induced irritation should be revised and may facilitate the development of smoking cessation therapies with less adverse effects...
  56. pmc Reduced intracellular ionic strength as the initial trigger for activation of endothelial volume-regulated anion channels
    T Voets
    Department of Physiology, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 96:5298-303. 1999
    ..Instead, they are in complete agreement with a model in which a decrease of Gammai rather than an increase in cell volume is the initial trigger for activation of ICl,swell...
  57. ncbi request reprint Abnormal intracellular ca(2+)homeostasis and disease
    L Missiaen
    Laboratory of Physiology, K U Leuven Campus Gasthuisberg O N, Leuven, Belgium
    Cell Calcium 28:1-21. 2000
    ..In some instances, disease-causing mutations or abnormalities provide us with new insights into the cell biology of the various Ca(2+)transporters...
  58. pmc Alternative splicing of ClC-6 (a member of the CIC chloride-channel family) transcripts generates three truncated isoforms one of which, ClC-6c, is kidney-specific
    J Eggermont
    Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, B 3000 Leuven, Belgium
    Biochem J 325:269-76. 1997
    ..In contrast, expression of ClC-6c is more restricted, since it was only detected in kidney...
  59. ncbi request reprint Calcium signalling through nucleotide receptor P2Y2 in cultured human vascular endothelium
    F Viana
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Belgium
    Cell Calcium 24:117-27. 1998
    ..These results indicate that P2Y2 receptors linked to intracellular Ca2+ mobilization are present in human vascular endothelial cells. The initial [Ca2+]i mobilization is followed by a phase of elevated [Ca2+]i influx...
  60. ncbi request reprint Current understanding of mammalian TRP homologues
    R Vennekens
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Leuven, Belgium
    Cell Calcium 31:253-64. 2002
    ..This review summarizes the currently available information concerning members of the TRP family expressed in mammalian tissues...
  61. ncbi request reprint TRPM8-independent menthol-induced Ca2+ release from endoplasmic reticulum and Golgi
    Frank Mahieu
    Department of Molecular Cell Biology, Division of Physiology, Laboratory of Ion Channel Research, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 282:3325-36. 2007
    ..Finally, aequorin-based measurements demonstrate that the TRPM8-independent menthol-induced Ca2+ release originates from both endoplasmic reticulum and Golgi compartments...
  62. ncbi request reprint Activation of TRPV4 channels (hVRL-2/mTRP12) by phorbol derivatives
    Hiroyuki Watanabe
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:13569-77. 2002
    ..We concluded that the phorbol derivatives activate TRPV4 (VR-OAC, VRL-2, OTRPC4, TRP12) independently from protein kinase C, in a manner consistent with direct agonist gating of the channel...
  63. ncbi request reprint Comparison of functional properties of the Ca2+-activated cation channels TRPM4 and TRPM5 from mice
    Nina D Ullrich
    Laboratorium voor Fysiologie, Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Cell Calcium 37:267-78. 2005
    ..TRPM4 displayed a 10-fold higher affinity for block by flufenamic acid. Importantly, ATP4- blocked TRPM4 with high affinity (IC50 of 0.8+/-0.1 microM), whereas TRPM5 is insensitive to ATP4- at concentrations up to 1 mM...
  64. pmc Permeation properties and modulation of volume-activated Cl(-)-currents in human endothelial cells
    B Nilius
    K U Leuven, Laboratorium voor Fysiologie, Belgium
    Br J Pharmacol 112:1049-56. 1994
    ..Evidence that they belong to the class of P-glycoprotein associated Cl--channels is however only marginal, whereas their biophysical characteristics differ significantly from those of the CIC-2 volume-activated Cl--channels...
  65. ncbi request reprint Aspartate residues of the Glu-Glu-Asp-Asp (EEDD) pore locus control selectivity and permeation of the T-type Ca(2+) channel alpha(1G)
    K Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
    J Biol Chem 276:45628-35. 2001
    ..The mutation of both divergent aspartates only partially confers HVA channel permeation properties to the alpha(1G) Ca(2+) channel subunit...
  66. ncbi request reprint Regulation of TRP channels: a voltage-lipid connection
    B Nilius
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem Soc Trans 35:105-8. 2007
    ..A PIP(2)-binding pocket seems to comprise a part of the TRP domain and especially pleckstrin homology domains in the C-terminus...
  67. ncbi request reprint Structure-function relationship of the TRP channel superfamily
    G Owsianik
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Herestraat 49, 3000 Leuven, Belgium
    Rev Physiol Biochem Pharmacol 156:61-90. 2006
    ..This review summarizes and discusses the current knowledge on the TRP protein structure and its impact on the regulation of the channel function...
  68. ncbi request reprint TRPV channels and modulation by hepatocyte growth factor/scatter factor in human hepatoblastoma (HepG2) cells
    Joris Vriens
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit, B 3000 Leuven, Belgium
    Cell Calcium 36:19-28. 2004
    ..HGF/SF increases Ca(2+) entry via TRPV1, but not via TRPV4. This rise in [Ca(2+)](i) may constitute an early response of a signalling cascade that gives rise to cell locomotion and the migratory phenotype...
  69. ncbi request reprint Heat activation of TRPM5 underlies thermal sensitivity of sweet taste
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    Nature 438:1022-5. 2005
    ....
  70. ncbi request reprint Anandamide and arachidonic acid use epoxyeicosatrienoic acids to activate TRPV4 channels
    Hiroyuki Watanabe
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    Nature 424:434-8. 2003
    ..Activation of TRPV4 in vascular endothelial cells might therefore contribute to the relaxant effects of endocannabinoids and their P450 epoxygenase-dependent metabolites on vascular tone...
  71. ncbi request reprint Molecular determinants of permeation through the cation channel TRPV4
    Thomas Voets
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    J Biol Chem 277:33704-10. 2002
    ..Our findings delineate the pore region of TRPV4 and give a first insight into the possible architecture of its permeation pathway...
  72. ncbi request reprint Diversity of TRP channel activation
    Bernd Nilius
    KU Leuven, Department of Physiology, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Novartis Found Symp 258:140-9; discussion 149-59, 263-6. 2004
    ..Regulation of channel availability by interaction with a protein bound to a site at the C-terminus of both channels will be demonstrated. Functional consequences of these different mechanisms of gating will be discussed...
  73. pmc Functional characterization of transient receptor potential channels in mouse urothelial cells
    Wouter Everaerts
    Department of Molecular Cell Biology, Laboratory Ion Channel Research, Campus Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium
    Am J Physiol Renal Physiol 298:F692-701. 2010
    ..These channels may contribute to the (mechano)sensory function of the urothelial layer and represent potential targets for the treatment of bladder dysfunction...
  74. pmc TRPA1 acts as a cold sensor in vitro and in vivo
    Yuji Karashima
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg O and N1, Katholieke Universiteit Leuven, Herestraat 49 bus 802, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 106:1273-8. 2009
    ..Finally, cold plate and tail-flick experiments reveal TRPA1-dependent, cold-induced nociceptive behavior in mice. We conclude that TRPA1 acts as a major sensor for noxious cold...
  75. ncbi request reprint Stimulation by caveolin-1 of the hypotonicity-induced release of taurine and ATP at basolateral, but not apical, membrane of Caco-2 cells
    Nina Ullrich
    Laboratory of Physiology, Campus Gasthuisberg, O and N, Herestraat 49, PO Box 802, B 3000 Leuven, Belgium
    Am J Physiol Cell Physiol 290:C1287-96. 2006
    ..It is therefore concluded that caveolin-1 facilitates the hypotonicity-induced release of Cl(-), taurine, and ATP, and that in polarized epithelial cells, the effect of caveolin-1 is compartmentalized to the basolateral membrane...
  76. ncbi request reprint Chronic exposure to EGF affects trafficking and function of ENaC channel in cystic fibrosis cells
    Lishuang Cao
    Department of Physiology, KU Leuven, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Biochem Biophys Res Commun 331:503-11. 2005
    ..Thus, in JME/CF15 cells inhibition of the ENaC function by chronic incubation with EGF is a Ca(2+)-mediated process that affects trafficking and surface expression of ENaC channels...
  77. doi request reprint Regulation of the murine TRPP3 channel by voltage, pH, and changes in cell volume
    Takahiro Shimizu
    Department of Molecular Cell Biology, Laboratory of Ion Channel Research, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat 49, Bus 802, B 3000, Leuven, Belgium
    Pflugers Arch 457:795-807. 2009
    ..Taken together, our results demonstrate that TRPP3, on its own, can act as a voltage-dependent, pH- and volume-sensitive plasma membrane cation channel...
  78. ncbi request reprint ATP and nitric oxide modulate a Ca(2+)-activated non-selective cation current in macrovascular endothelial cells
    Suk H Suh
    Katholieke Universiteit Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, 3000 Leuven, Belgium
    Pflugers Arch 444:438-45. 2002
    ..It is concluded that the Ca(2+)-activated non-selective action channel in EA cells is modulated by the metabolic state of the cell and by NO...
  79. ncbi request reprint Characterization of 19 disease-associated missense mutations in the regulatory domain of the cystic fibrosis transmembrane conductance regulator
    A Vankeerberghen
    Centre for Human Genetics and Department of Physiology, University of Leuven, B 3000 Leuven, Belgium
    Hum Mol Genet 7:1761-9. 1998
    ..Two mutations, H620Q and A800G, resulted in increased intrinsic chloride transport activities. Finally, T665S and E826K CFTR had single channel properties not significantly different from wild-type CFTR...
  80. pmc Polyvariant mutant cystic fibrosis transmembrane conductance regulator genes. The polymorphic (Tg)m locus explains the partial penetrance of the T5 polymorphism as a disease mutation
    H Cuppens
    Center for Human Genetics, KULeuven, B 3000 Leuven, Belgium
    J Clin Invest 101:487-96. 1998
    ..Such polyvariant mutant genes could explain why apparently normal CFTR genes cause disease. Moreover, they might be responsible for variation in the phenotypic expression of CFTR mutations, and be of relevance in other genetic diseases...
  81. ncbi request reprint Do voltage-gated Kv1.1 and inward rectifier Kir2.1 potassium channels form heteromultimers?
    J Tytgat
    University of Leuven, Laboratory of Toxicology, Belgium
    FEBS Lett 390:280-4. 1996
    ..We conclude that Kv1.1 and Kir2.1 alpha-subunit proteins fail to assemble and do not contribute functional diversity to K+ channels...
  82. doi request reprint The loss and progressive recovery of voiding after spinal cord interruption in rats is associated with simultaneous changes in autonomous contractile bladder activity
    Thomas Gevaert
    KU Leuven, Department of Urology, University Hospitals Gasthuisberg, Leuven, Belgium
    Eur Urol 56:168-76. 2009
    ..Autonomous contractile activity (ACA) is a well-known phenomenon in isolated bladders from different species and seems to be important in the physiology of both normal and dysfunctional voiding...
  83. pmc Modulation of the cold-activated cation channel TRPM8 by surface charge screening
    Frank Mahieu
    KU Leuven, Laboratory of Ion Channel Research, Department of Molecular Cell Biology, Leuven, Belgium
    J Physiol 588:315-24. 2010
    ..These results represent the first description of the effects of surface charge screening on a TRP channel and provide a straightforward explanation for the known effects of extracellular Ca(2+) on cold-sensitive neurons...
  84. ncbi request reprint TRPM8 voltage sensor mutants reveal a mechanism for integrating thermal and chemical stimuli
    Thomas Voets
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg O and N1, KU Leuven, Herestraat 49 bus 802, B 3000 Leuven, Belgium
    Nat Chem Biol 3:174-82. 2007
    ..Based on these findings, we present a Monod-Wyman-Changeux-type model explaining the combined effects of voltage, temperature and menthol on TRPM8 gating...
  85. doi request reprint Transient receptor potential channels in sensory neurons are targets of the antimycotic agent clotrimazole
    Victor Meseguer
    Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg O and N1, KU Leuven, B 3000 Leuven, Belgium
    J Neurosci 28:576-86. 2008
    ..Together, our results identify TRP channels in sensory neurons as molecular targets of CLT, and offer means to develop novel CLT preparations with fewer unwanted sensory side effects...
  86. pmc Loss of high-frequency glucose-induced Ca2+ oscillations in pancreatic islets correlates with impaired glucose tolerance in Trpm5-/- mice
    Barbara Colsoul
    Laboratory of Ion Channel Research and Gene Expression Unit, Department of Molecular Cell Biology, Katholieke Universiteit Leuven, B 3001 Louvain, Belgium
    Proc Natl Acad Sci U S A 107:5208-13. 2010
    ..As a consequence, glucose-induced insulin release from Trpm5(-/-) pancreatic islets is significantly reduced, resulting in an impaired glucose tolerance in Trpm5(-/-) mice...
  87. ncbi request reprint Biophysics and structure-function relationship of T-type Ca2+ channels
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    Cell Calcium 40:97-114. 2006
    ..Certainly, more structure-function studies are needed for a better understanding of T-type channel physiology and the rational design of treatments against T-type channel-related pathologies...
  88. doi request reprint Maturation of stretch-induced contractile activity and its muscarinic regulation in isolated whole bladder strips from rat
    Thomas Gevaert
    Department of Urology, University Hospitals Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium
    Neurourol Urodyn 29:789-96. 2010
    ..Here we explored the behavior of stretch-induced contractile activity (SIC) and its muscarinic regulation in neonatal and adult rat bladders...
  89. doi request reprint Modulation of the transient receptor potential channel TRPA1 by phosphatidylinositol 4,5-biphosphate manipulators
    Yuji Karashima
    KU Leuven Department of Mol Cell Biology, Lab Ion Channel Research, Campus Gasthuisberg, Herestraat 49, Bus 802, B 3000, Leuven, Belgium
    Pflugers Arch 457:77-89. 2008
    ....
  90. ncbi request reprint Sensing with TRP channels
    Thomas Voets
    Laboratory of Physiology, Campus Gasthuisberg O and N, KU Leuven, B 3000 Leuven, Belgium
    Nat Chem Biol 1:85-92. 2005
    ..Activation of TRP channels is highly sensitive to a variety of chemical and physical stimuli, allowing them to function as dedicated biological sensors that are essential in processes such as vision, taste, tactile sensation and hearing...
  91. pmc Agonist-induced changes in Ca(2+) permeation through the nociceptor cation channel TRPA1
    Yuji Karashima
    Department of Molecular Cell Biology, Katholieke Universiteit Leuven, Belgium
    Biophys J 98:773-83. 2010
    ..We conclude that the pore of TRPA1 is dynamic and supports a surprisingly large Ca(2+) influx...
  92. pmc Mg2+-dependent gating and strong inward rectification of the cation channel TRPV6
    Thomas Voets
    Department of Physiology, Campus Gasthuisberg, KU Leuven, Belgium
    J Gen Physiol 121:245-60. 2003
    ..The effects of intracellular Mg(2+) on TRPV6 are partially reminiscent of the gating mechanism of inwardly rectifying K(+) channels and may represent a novel regulatory mechanism for TRPV6 function in vivo...
  93. ncbi request reprint Functional analysis of CFTR chloride channel activity in cells with elevated MDR1 expression
    Lishuang Cao
    Laboratory of Physiology, Catholic University of Leuven, Campus Gasthuisberg, Herestraat 49, B 3000, Leuven, Belgium
    Biochem Biophys Res Commun 304:248-52. 2003
    ..This indicated that repression of CFTR by MDR1 induction requires the presence of the native CFTR promoter...
  94. pmc Pore structure influences gating properties of the T-type Ca2+ channel alpha1G
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Belgium
    J Gen Physiol 121:529-40. 2003
    ..These changes are independent of the alterations of the selectivity properties and channel protonation...
  95. pmc Extracellular Ca2+ modulates the effects of protons on gating and conduction properties of the T-type Ca2+ channel alpha1G (CaV3.1)
    Karel Talavera
    Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Belgium
    J Gen Physiol 121:511-28. 2003
    ....
  96. ncbi request reprint The pore of TRP channels: trivial or neglected?
    Thomas Voets
    Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Cell Calcium 33:299-302. 2003
    ....
  97. ncbi request reprint Modulation of TRPV4 gating by intra- and extracellular Ca2+
    Hiroyuki Watanabe
    Department of Physiology, Laboratorium voor Fysiologie, Campus Gasthuisberg, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Cell Calcium 33:489-95. 2003
    ....
  98. ncbi request reprint The intracellular tyrosine residues of the ATP-gated P2X(1) ion channel are essential for its function
    Emese Toth-Zsamboki
    Center for Molecular and Vascular Biology, University of Leuven, Herestraat 49, 3000 Leuven, Belgium
    FEBS Lett 524:15-9. 2002
    ..Thus, Y16, Y362, Y363 and Y370 are required for the appropriate three-dimensional structure and function of the intracellular P2X(1) domains...
  99. ncbi request reprint TRPM6 forms the Mg2+ influx channel involved in intestinal and renal Mg2+ absorption
    Thomas Voets
    Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 279:19-25. 2004
    ..Our data indicate that TRPM6 comprises all or part of the apical Mg2+ channel of Mg2+-absorbing epithelia...
  100. ncbi request reprint Rescue of functional DeltaF508-CFTR channels by co-expression with truncated CFTR constructs in COS-1 cells
    Grzegorz Owsianik
    Laboratory of Physiology, Catholic University of Leuven, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    FEBS Lett 554:173-8. 2003
    ..In conclusions, our data demonstrated that expression of truncated CFTR constructs could be a novel promising approach to improve maturation of DeltaF508-CFTR channels...
  101. ncbi request reprint Outer pore architecture of a Ca2+-selective TRP channel
    Thomas Voets
    Department of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    J Biol Chem 279:15223-30. 2004
    ..However, the selectivity filter of TRPV6 is wider than that of K(+) channels and lined by amino acid side chains rather than main chain carbonyls...