B Nilius


Affiliation: Katholieke Universiteit Leuven
Country: Belgium


  1. Nilius B, Voets T, Prenen J, Barth H, Aktories K, Kaibuchi K, et al. Role of Rho and Rho kinase in the activation of volume-regulated anion channels in bovine endothelial cells. J Physiol. 1999;516 ( Pt 1):67-74 pubmed
    ..9. These experiments provide strong evidence that the Rho-Rho kinase pathway is involved in the VRAC activation cascade. ..
  2. Nilius B, Prenen J, Voets T, Eggermont J, Droogmans G. Activation of volume-regulated chloride currents by reduction of intracellular ionic strength in bovine endothelial cells. J Physiol. 1998;506 ( Pt 2):353-61 pubmed
    ..7. It is concluded that a reduction of gamma 1 activates an anion current that is identical to that activated by cell swelling. Changes in intracellular ionic strength may shift the volume set point for activation of ICl,swell. ..
  3. Nilius B, Vriens J, Prenen J, Droogmans G, Voets T. TRPV4 calcium entry channel: a paradigm for gating diversity. Am J Physiol Cell Physiol. 2004;286:C195-205 pubmed
    ..This review summarizes data on TRPV4 as a paradigm of gating diversity in this subfamily of Ca2+ entry channels. ..
  4. Nilius B, Vennekens R, Prenen J, Hoenderop J, Droogmans G, Bindels R. The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel. J Biol Chem. 2001;276:1020-5 pubmed publisher
    ..Mutations of two neighboring negatively charged residues, i.e. Glu(535) and Asp(550), had only minor effects on Ca(2+) permeation properties...
  5. Nilius B, Droogmans G. Ion channels and their functional role in vascular endothelium. Physiol Rev. 2001;81:1415-59 pubmed
  6. Nilius B, Prenen J, Hoenderop J, Vennekens R, Hoefs S, Weidema A, et al. Fast and slow inactivation kinetics of the Ca2+ channels ECaC1 and ECaC2 (TRPV5 and TRPV6). Role of the intracellular loop located between transmembrane segments 2 and 3. J Biol Chem. 2002;277:30852-8 pubmed
    ..In conclusion, the TM2-TM3 loop is a critical determinant of the inactivation in ECaC1 and ECaC2. ..
  7. Nilius B, Weidema F, Prenen J, Hoenderop J, Vennekens R, Hoefs S, et al. The carboxyl terminus of the epithelial Ca(2+) channel ECaC1 is involved in Ca(2+)-dependent inactivation. Pflugers Arch. 2003;445:584-8 pubmed publisher
    ..In conclusion, we have identified two domains in the carboxyl terminus of ECaC1 that control Ca(2+)-dependent inactivation...
  8. Nilius B, Prenen J, Droogmans G, Voets T, Vennekens R, Freichel M, et al. Voltage dependence of the Ca2+-activated cation channel TRPM4. J Biol Chem. 2003;278:30813-20 pubmed
    ..Voltage dependence of TRPM4 may be functionally important, especially in excitable tissues generating plateau-like or bursting action potentials. ..
  9. Nilius B, Prenen J, Voets T, Droogmans G. Intracellular nucleotides and polyamines inhibit the Ca2+-activated cation channel TRPM4b. Pflugers Arch. 2004;448:70-5 pubmed
    ..Spermine blocked TRPM4 currents with an IC(50) of 61 microM. In conclusion, TRPM4 is a channel that can be effectively modulated by intracellular nucleotides and polyamines. ..

More Information


  1. Nilius B, Prenen J, Janssens A, Voets T, Droogmans G. Decavanadate modulates gating of TRPM4 cation channels. J Physiol. 2004;560:753-65 pubmed
    ..These results indicate that the C-terminus of TRPM4 is critically involved in mediating the DV effects. In conclusion, decavanadate modulates TRPM4, but not TRPM5, by inhibiting voltage-dependent closure of the channel. ..
  2. Nilius B, Prenen J, Tang J, Wang C, Owsianik G, Janssens A, et al. Regulation of the Ca2+ sensitivity of the nonselective cation channel TRPM4. J Biol Chem. 2005;280:6423-33 pubmed
    ..Thus, the Ca(2+) sensitivity of TRPM4 is regulated by ATP, PKC-dependent phosphorylation, and calmodulin binding at the C terminus. ..
  3. Nilius B, Prenen J, Janssens A, Owsianik G, Wang C, Zhu M, et al. The selectivity filter of the cation channel TRPM4. J Biol Chem. 2005;280:22899-906 pubmed
    ..Our findings delineate the selectivity filter of TRPM channels and provide the first insight into the molecular basis of monovalent cation selectivity. ..
  4. Nilius B, Mahieu F, Prenen J, Janssens A, Owsianik G, Vennekens R, et al. The Ca2+-activated cation channel TRPM4 is regulated by phosphatidylinositol 4,5-biphosphate. EMBO J. 2006;25:467-78 pubmed
    ..Our data demonstrate that PIP2 is a strong positive modulator of TRPM4, and implicate the C-terminal PH domain in PIP2 action. PLC-mediated PIP2 breakdown may constitute a physiologically important brake on TRPM4 activity. ..