Affiliation: Katholieke Universiteit Leuven
- Channels involved in transient currents unmasked by removal of extracellular calcium in cardiac cellsRegina Macianskiene
Laboratory of Cardiac Cellular Research, Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Belgium
Am J Physiol Heart Circ Physiol 282:H1879-88. 2002..They could be increased by BAY K-8644 or by isoproterenol. We conclude that the I(to)-like currents are due to monovalent cation flow through L-type Ca(2+) channels, which in pig myocytes show low sensitivity to nifedipine...
- Mechanisms underlying the frequency dependence of contraction and [Ca(2+)](i) transients in mouse ventricular myocytesGudrun Antoons
Laboratory of Experimental Cardiology, University of Leuven, Belgium
J Physiol 543:889-98. 2002..Small changes in this balance may contribute to variability in frequency-dependent behaviour. In addition, there may be a regulation of the contractile response downstream of [Ca(2+)](i)...
- Spatial and temporal inhomogeneities during Ca2+ release from the sarcoplasmic reticulum in pig ventricular myocytesFrank R Heinzel
Laboratory of Experimental Cardiology, University of Leuven, Leuven, Belgium
Circ Res 91:1023-30. 2002..This lower number of functional couplons contributes to a slower overall rate of rise of [Ca2+]i...
- Increased phospholamban phosphorylation limits the force-frequency response in the MLP-/- mouse with heart failureGudrun Antoons
Laboratory of Experimental Cardiology, University of Leuven, Campus Gasthuisberg O N 704, Herestraat 49, B 3000 Leuven, Belgium
J Mol Cell Cardiol 40:350-60. 2006..This represents a novel paradigm for altered Ca(2+) handling in heart failure, underscoring the importance of phosphorylation pathways...
- Calcium uptake by the sarcoplasmic reticulum, high energy content and histological changes in ischemic cardiomyopathyK Mubagwa
Laboratory of Experimental Cardiac Surgery, Katholieke Universiteit Leuven, Belgium
Cardiovasc Res 37:515-23. 1998..Sarcoplasmic reticulum (SR) Ca2+ uptake, myocardial high energy content and histology were examined in different zones of hearts from patients with ischemic cardiomyopathy...
- KB130015, a new amiodarone derivative with multiple effects on cardiac ion channelsKanigula Mubagwa
Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Belgium
Cardiovasc Drug Rev 21:216-35. 2003..At high concentrations KB015 exerts a positive inotropic action. It also interacts with thyroid hormone nuclear receptors. Its toxic effects remain largely unexplored, but it is well tolerated during chronic administration...
- Adenosine, adenosine receptors and myocardial protection: an updated overviewK Mubagwa
Centre for Experimental Surgery and Anaesthesiology, University of Leuven, 3000, Leuven, Belgium
Cardiovasc Res 52:25-39. 2001..In addition, more studies are needed to clarify the signalling mechanisms by which A(3) receptor activation or overexpression may promote apoptosis and cellular injury, as reported by a few recent studies...
- Action potential changes associated with a slowed inactivation of cardiac voltage-gated sodium channels by KB130015R Macianskiene
Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Leuven B 3000, Belgium
Br J Pharmacol 139:1469-79. 2003..6 It is suggested that a prolongation of short action potentials by drugs or mutations that only slow I(Na) inactivation does not necessarily imply identical changes in other species or in different myocardial regions...
- Suppression of transient outward potassium currents in mouse ventricular myocytes by imidazole antimycotics and by glybenclamideM J Hernandez-Benito
Laboratory of Cardiac Cellular Research, Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Leuven, Belgium
J Pharmacol Exp Ther 298:598-606. 2001..These data demonstrate a pharmacological separation of I(to) components using antimycotic drugs but not glybenclamide...
- Extracellular divalent cations block a cation non-selective conductance unrelated to calcium channels in rat cardiac muscleK Mubagwa
Centrum voor Experimentele Heelkunde en Anesthesiologie, University of Leuven, Belgium
J Physiol 502:235-47. 1997..6. These results suggest that a novel conductance pathway, permeable to monovalent cations but not to Cl- and blocked by divalent cations, exists in ventricular myocytes...
- Improved functional recovery after ischemic preconditioning in the globally ischemic rabbit heart is not mediated by adenosine A1 receptor activationM Hendrikx
Laboratory for Experimental Cardiac Surgery, Katholieke Universiteit Leuven, Belgium
Basic Res Cardiol 88:576-93. 1993..Administration of exogenous adenosine reduces functional impairment to a certain extent, but less than preconditioning...
- Modulation of the extracellular divalent cation-inhibited non-selective conductance in cardiac cells by metabolic inhibition and by oxidantsR Macianskiene
Laboratory of Cardiac Cellular Research, Centre for Experimental Surgery and Anaesthesiology, Katholieke Universiteit Leuven, Campus Gasthuisberg, Leuven, B-3000, Belgium
J Mol Cell Cardiol 33:1371-85. 2001....
- Induction of a novel cation current in cardiac ventricular myocytes by flufenamic acid and related drugsR Macianskiene
Division of Experimental Cardiac Surgery, Department of Cardiovascular Diseases, University of Leuven, Leuven, Belgium
Br J Pharmacol 161:416-29. 2010..Interest in non-selective cation channels has increased recently following the discovery of transient receptor potential (TRP) proteins, which constitute many of these channels...
- Antiarrhythmic drugs and cardiac ion channels: mechanisms of actionE Carmeliet
Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Belgium
Prog Biophys Mol Biol 70:1-72. 1998..Finally, the question is asked as to whether the available drugs by their action on channels and receptors modify the vulnerable parameter in the desired way to stop or prevent arrhythmias...
- Deterioration of the protein kinase C-K(ATP) channel pathway in regulation of coronary flow in hypercholesterolaemic rabbitsE Pongo
Centre of Experimental Surgery and Anaesthesiology, Katholieke Universiteit Minderbroedenstraat 17, B-3000, Leuven, Belgium
Eur J Pharmacol 418:217-23. 2001..We conclude that non-cholesterol mevalonate products are necessary for the functional integrity of the protein kinase C-K(ATP) channel pathway in the rabbit heart...
- Slowing of the inactivation of cardiac voltage-dependent sodium channels by the amiodarone derivative 2-methyl-3-(3,5-diiodo-4-carboxymethoxybenzyl)benzofuran (KB130015)R Macianskiene
Centre for Experimental Surgery and Anaesthesiology, University of Leuven, Leuven, Belgium
J Pharmacol Exp Ther 304:130-8. 2003..These data suggest the existence of a binding site for KB and T3 on Na+ channels...
- [Na(+)] in the subsarcolemmal 'fuzzy' space and modulation of [Ca(2+)](i) and contraction in cardiac myocytesFons Verdonck
Interdisciplinary Research Center, Katholieke University of Leuven, Campus Kortrijk, Kortrijk, Belgium
Cell Calcium 35:603-12. 2004..This has implications for modulation of [Ca(2+)](i) during a single beat as well as during alterations in Na(+) fluxes seen in pathological conditions...
- The amiodarone derivative KB130015 [2-methyl-3-(3,5-diiodo-4-carboxymethoxybenzyl)benzofuran] induces an Na+-dependent increase of [Ca2+] in ventricular myocytesVirginie Bito
Laboratory of Experimental Cardiology, Catholic University Leuven, Campus Gasthuisberg O N 7th Floor, Herestraat 49, B 3000 Leuven, Belgium
J Pharmacol Exp Ther 316:162-8. 2006..This effect is partially offset by a decrease in I(CaL) resulting in a mild inotropic effect without the signs of Ca(2+) overload and related arrhythmias usually associated with Na(+) channel openers...
- Inhibition of the magnesium-sensitive TRPM7-like channel in cardiac myocytes by nonhydrolysable GTP analogs: involvement of phosphoinositide metabolismRegina Macianskiene
Department of Cardiovascular Diseases, Katholieke Universiteit Leuven, Leuven, Belgium
Cell Physiol Biochem 22:109-18. 2008..A magnesium-inhibited, transient receptor potential melastatin 7 (TRPM7)-like channel is expressed in cardiac cell membranes. The role and regulation of this channel by intracellular nucleotides and membrane components remain unclear...
- Regulation of cation channels in cardiac and smooth muscle cells by intracellular magnesiumKanigula Mubagwa
Division of Experimental Cardiac Surgery, Department of Heart and Vessel Diseases, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
Arch Biochem Biophys 458:73-89. 2007..Modifications of cardiovascular electrical or mechanical function, possibly resulting in arrhythmias or hypertension, may result from such changes of Mg(i)(2+) and their effects on cation channels...
- ATP and PIP2 dependence of the magnesium-inhibited, TRPM7-like cation channel in cardiac myocytesAsfree Gwanyanya
Experimental Cardiac Surgery, Heart and Vessel Diseases, Katholieke Universiteit Leuven, Leuven, Belgium
Am J Physiol Cell Physiol 291:C627-35. 2006..Finally, loading ATP-depleted cells with exogenous PIP(2) (10 microM) prevented rundown. These results suggest that PIP(2), likely generated by ATP-utilizing lipid kinases, is necessary for maintaining cardiac MIC channel activity...
- Magnesium-inhibited, TRPM6/7-like channel in cardiac myocytes: permeation of divalent cations and pH-mediated regulationAsfree Gwanyanya
Centre for Experimental Surgery and Anaesthesiology, Katholieke Universiteit, Leuven, Belgium
J Physiol 559:761-76. 2004..Our results suggest that IMIC in cardiac cells is due to TRPM channels, most probably to TRPM6 or TRPM7 channels or to their heteromultimeres...
- Reduced synchrony of Ca2+ release with loss of T-tubules-a comparison to Ca2+ release in human failing cardiomyocytesWilliam E Louch
Laboratory of Experimental Cardiology, University of Leuven, Campus Gasthuisberg O N 7th Floor, Herestraat 49, B 3000 Leuven, Belgium
Cardiovasc Res 62:63-73. 2004..A partial loss of T-tubules has been described in myocytes from failing canine and human hearts. We examined how graded reduction of T-tubule density would affect the synchrony of Ca2+ release...
- Monovalent cation (MC) current in cardiac and smooth muscle cells: regulation by intracellular Mg2+ and inhibition by polycationsSergey I Zakharov
Vascular Biology Unit, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, U S A
Br J Pharmacol 138:234-44. 2003..7 It is concluded that MC current could be regulated by intracellular Mg(2+), and low concentrations of extracellular spermine could be used to discriminate it from SOC current, and to assess its role in cellular function...
- Overexpression of the Na/Ca exchanger shapes stimulus-induced cytosolic Ca(2+) oscillations in insulin-producing BRIN-BD11 cellsFrançoise Van Eylen
Laboratory of Pharmacology, Brussels University School of Medicine, Brussels, Belgium
Diabetes 51:366-75. 2002....
- Does adenosine protect the heart by acting on the sarcoplasmic reticulum?Kanigula Mubagwa
Cardiovasc Res 53:286-9. 2002