H Roger Lijnen

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Enzyme-linked immunosorbent assay for the specific detection of angiostatin-like plasminogen moieties in biological samples
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000, Louvain, Belgium
    Thromb Res 102:53-9. 2001
  2. ncbi request reprint Accelerated neointima formation after vascular injury in mice with stromelysin-3 (MMP-11) gene inactivation
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 19:2863-70. 1999
  3. ncbi request reprint Specific proteolysis of human plasminogen by a 24 kDa endopeptidase from a novel Chryseobacterium Sp
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Biochemistry 39:479-88. 2000
  4. ncbi request reprint Alpha(2)-antiplasmin gene deficiency in mice does not affect neointima formation after vascular injury
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 20:1488-92. 2000
  5. ncbi request reprint Neointima formation and thrombosis after vascular injury in transgenic mice overexpressing plasminogen activator inhibitor-1 (PAI-1)
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    J Thromb Haemost 2:16-22. 2004
  6. ncbi request reprint CD9 gene deficiency does not affect smooth muscle cell migration and neointima formation after vascular injury in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 83:956-61. 2000
  7. ncbi request reprint Inactivation of plasminogen activator inhibitor-1 by specific proteolysis with stromelysin-1 (MMP-3)
    H R Lijnen
    Center for Molecular and Vascular Biology and Laboratory for Pharmaceutical Biology and Phytopharmacology, University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 275:37645-50. 2000
  8. ncbi request reprint Deficiency of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) impairs nutritionally induced obesity in mice
    H Roger Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Thromb Haemost 89:249-55. 2003
  9. ncbi request reprint Gene targeting in hemostasis. Alpha2-antiplasmin
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Campus, Gasthuisberg, O and N, Herestraat 49, B 3000 Leuven, Belgium
    Front Biosci 6:D239-47. 2001
  10. ncbi request reprint Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inhibitor-1
    H Roger Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Arterioscler Thromb Vasc Biol 23:78-84. 2003

Collaborators

Detail Information

Publications117 found, 100 shown here

  1. ncbi request reprint Enzyme-linked immunosorbent assay for the specific detection of angiostatin-like plasminogen moieties in biological samples
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000, Louvain, Belgium
    Thromb Res 102:53-9. 2001
    ..This ELISA thus appears suitable for the specific quantitation of angiostatin-like plasminogen moieties in biological samples, and may be useful to study its (patho)physiological relevance...
  2. ncbi request reprint Accelerated neointima formation after vascular injury in mice with stromelysin-3 (MMP-11) gene inactivation
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 19:2863-70. 1999
    ..8% at 3 weeks, P<0. 005). The mechanisms by which MMP-11 could impair elastin degradation and cellular migration in this model remain, however, unknown...
  3. ncbi request reprint Specific proteolysis of human plasminogen by a 24 kDa endopeptidase from a novel Chryseobacterium Sp
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Biochemistry 39:479-88. 2000
    ..Interaction of this novel 24k-endopeptidase with plasminogen thus yields an angiostatin-like fragment and affects plasmin-mediated cellular proteolytic activity...
  4. ncbi request reprint Alpha(2)-antiplasmin gene deficiency in mice does not affect neointima formation after vascular injury
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 20:1488-92. 2000
    ....
  5. ncbi request reprint Neointima formation and thrombosis after vascular injury in transgenic mice overexpressing plasminogen activator inhibitor-1 (PAI-1)
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    J Thromb Haemost 2:16-22. 2004
    ..These findings indicate that in a vascular injury model that induces extensive and persistent fibrin deposition in femoral arteries of mice, overexpression of PAI-1 does not affect neointima formation...
  6. ncbi request reprint CD9 gene deficiency does not affect smooth muscle cell migration and neointima formation after vascular injury in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 83:956-61. 2000
    ..Thus, in this model CD9 is highly expressed by migrating SMC, but deficiency of CD9 does not affect SMC migration or neointima formation after perivascular injury...
  7. ncbi request reprint Inactivation of plasminogen activator inhibitor-1 by specific proteolysis with stromelysin-1 (MMP-3)
    H R Lijnen
    Center for Molecular and Vascular Biology and Laboratory for Pharmaceutical Biology and Phytopharmacology, University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 275:37645-50. 2000
    ..Cleavage and inactivation of PAI-1 by MMP-3 may thus constitute a mechanism decreasing the antiproteolytic activity of PAI-1 and impairing the potential inhibitory effect of vitronectin-bound PAI-1 on cell adhesion and/or migration...
  8. ncbi request reprint Deficiency of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) impairs nutritionally induced obesity in mice
    H Roger Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Thromb Haemost 89:249-55. 2003
    ..Thus, in a murine model of nutritionally induced obesity, TIMP-1 promotes adipose tissue development...
  9. ncbi request reprint Gene targeting in hemostasis. Alpha2-antiplasmin
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Campus, Gasthuisberg, O and N, Herestraat 49, B 3000 Leuven, Belgium
    Front Biosci 6:D239-47. 2001
    ..These data suggest that, at least in the murine system, the main role of alpha2-antiplasmin is in regulating plasmin activity in the circulating blood and in controlling intravascular fibrinolysis...
  10. ncbi request reprint Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inhibitor-1
    H Roger Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Arterioscler Thromb Vasc Biol 23:78-84. 2003
    ..The objective of this study was to investigate the role of plasminogen activator inhibitor-1 (PAI-1) in adipose tissue development in vivo...
  11. ncbi request reprint Inactivation of the serpin alpha(2)-antiplasmin by stromelysin-1
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Louvain, Campus Gasthuisberg, O and N, Herestraat 49, B 3000, Leuven, Belgium
    Biochim Biophys Acta 1547:206-13. 2001
    ..Cleavage and inactivation of alpha(2)-AP by MMP-3 may constitute a mechanism favoring local plasmin-mediated proteolysis...
  12. ncbi request reprint Adipose tissue expression of gelatinases in mouse models of obesity
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 85:1111-6. 2001
    ..The presence of MMP-2 in the adipose tissue was confirmed immunologically and its localization in adipocytes revealed by immunogold electron microscopy. The potential functional role of MMP-2 in adipose tissue remains to be determined...
  13. ncbi request reprint Elements of the fibrinolytic system
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Ann N Y Acad Sci 936:226-36. 2001
    ..Thereby, MMP-3 may downregulate cell associated plasmin activity by decreasing the amount of activatable plasminogen, without affecting cell bound u-PA activity...
  14. ncbi request reprint Plasmin and matrix metalloproteinases in vascular remodeling
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 86:324-33. 2001
    ..Thus, the plasminogen/plasmin and MMP systems, in concert, contribute to vascular remodeling in the setting of cardiovascular disease...
  15. ncbi request reprint Modulation of fibrinolytic and gelatinolytic activity during adipose tissue development in a mouse model of nutritionally induced obesity
    H Roger Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000 Leuven, Belgium
    Thromb Haemost 88:345-53. 2002
    ..05). The fibrillar collagen content was lower in adipose tissue of mice on HFD. Thus, in this model time-dependent development of adipose tissue appears to be associated with modulation of proteolytic activity...
  16. ncbi request reprint Extracellular proteolysis in the development and progression of atherosclerosis
    H Roger Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000 Leuven, Belgium
    Biochem Soc Trans 30:163-7. 2002
    ....
  17. ncbi request reprint Matrix metalloproteinases and cellular fibrinolytic activity
    H Roger Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000 Leuven, Belgium
    Biochemistry (Mosc) 67:92-8. 2002
    ..Furthermore, the temporal and topographic expression pattern of MMP components, as well as studies in gene-deficient mice, suggest a functional role in neointima formation after vascular injury...
  18. ncbi request reprint Tissue inhibitor of matrix metalloproteinases-1 impairs arterial neointima formation after vascular injury in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Circ Res 85:1186-91. 1999
    ..These data are compatible with a role of TIMP-1 in the impairment of SMC migration and neointima formation after vascular injury, as a result of inhibition of MMP activity...
  19. ncbi request reprint Temporal and topographic matrix metalloproteinase expression after vascular injury in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 81:799-807. 1999
    ..The temporal and topographic expression pattern of MMPs after vascular injury, coinciding with smooth muscle cell migration and neointima formation, thus is compatible with a role in vascular remodeling...
  20. ncbi request reprint Characterization of the murine plasminogen/urokinase-type plasminogen-activator system
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Eur J Biochem 241:840-8. 1996
    ..However, quantitative differences were observed both in the presence of cells and in plasma which may contribute to a reduced u-PA-mediated fibrinolytic activity in the murine systems...
  21. ncbi request reprint Plasminogen-dependent and -independent proteolytic activity of murine endothelioma cells with targeted inactivation of fibrinolytic genes
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 77:362-7. 1997
    ..These data indicate that the proteolytic activity of the transformed murine endothelioma cells, measured in plasminogen activation or matrix degradation assays, is essentially u-PA-related and largely plasminogen-dependent...
  22. ncbi request reprint On the role of plasminogen activator inhibitor-1 in adipose tissue development and insulin resistance in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Belgium
    J Thromb Haemost 3:1174-9. 2005
    ..To investigate the role of plasminogen activator inhibitor-1 (PAI-1) in adipose tissue development and insulin metabolism...
  23. ncbi request reprint Effect of plasminogen activator inhibitor-1 deficiency on nutritionally-induced obesity in mice
    H Roger Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Belgium
    Thromb Haemost 93:816-9. 2005
    ..Thus, this study in littermate mice on high fat diet did not reveal an effect of PAI-1 deficiency on body weight, and a differential effect on SC and GON adipose tissue...
  24. ncbi request reprint Generation of an angiostatin-like fragment from plasminogen by stromelysin-1 (MMP-3)
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Biochemistry 37:4699-702. 1998
    ..Thus, proteolytic cleavage of plasminogen by MMP-3 generates angiostatin-like fragments...
  25. ncbi request reprint Matrix metalloproteinase inhibition impairs adipose tissue development in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 22:374-9. 2002
    ..These data suggest a functional role for MMPs in the development of adipose tissue...
  26. ncbi request reprint Regulation of gelatinase activity in mice with targeted inactivation of components of the plasminogen/plasmin system
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 79:1171-6. 1998
    ..Activation of proMMP-9 was enhanced in the presence of plasmin(ogen), but active MMP-9 was also detected in fibroblasts of Plg(-/-) mice, indicating that in vivo activation may occur via plasmin(ogen)-independent mechanisms...
  27. ncbi request reprint Function of the plasminogen/plasmin and matrix metalloproteinase systems after vascular injury in mice with targeted inactivation of fibrinolytic system genes
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Arterioscler Thromb Vasc Biol 18:1035-45. 1998
    ....
  28. ncbi request reprint Metalloproteinases in development and progression of vascular disease
    H Roger Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Belgium
    Pathophysiol Haemost Thromb 33:275-81. 2003
    ..The MMP system may therefore represent a potential therapeutic target for treatment of restenosis or atherosclerosis...
  29. ncbi request reprint Pleiotropic functions of plasminogen activator inhibitor-1
    H R Lijnen
    Center for Molecular and Vascular Biology, KU, Leuven, Belgium
    J Thromb Haemost 3:35-45. 2005
    ..It is not always clear if these functions depend on the antiproteolytic activity of PAI-1, on its binding to vitronectin or on its intereference with cellular migration or matrix binding...
  30. ncbi request reprint Tiplaxtinin impairs nutritionally induced obesity in mice
    H Roger Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Box 911, Herestraat 49, B 3000 Leuven, Belgium
    Thromb Haemost 96:731-7. 2006
    ..03). Thus, in this model of diet-induced obesity in mice administration of tiplaxtinin resulted in impaired adipose tissue development...
  31. ncbi request reprint Enhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation
    Erik Maquoi
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000 Leuven, Belgium
    Thromb Haemost 89:696-704. 2003
    ..Thus, in a murine model of nutritionally induced obesity, MMP-3 impairs adipose tissue development, possibly by affecting food intake and/or adipose tissue-related angiogenesis...
  32. ncbi request reprint Prothrombotic effect of Rofecoxib in a murine venous thrombosis model
    Nobuo Nagai
    Center for Molecular and Vascular Biology, K U Leuven, Leuven, Belgium
    Thromb Res 122:668-73. 2008
    ..5+/-0.7 min versus 6.8+/-3.0 min). Thus, an enhanced prothrombotic effect of Rofecoxib was detected when using a venous thrombosis model in lean mice, but not when using an arterial thrombosis model in obese mice...
  33. ncbi request reprint Modulation of angiogenesis during adipose tissue development in murine models of obesity
    Gabor Voros
    Center for Molecular and Vascular Biology, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs en Navorsing, Herestraat 49, B 3000 Leuven, Belgium
    Endocrinology 146:4545-54. 2005
    ..This modulation of angiogenic factors during development of adipose tissue supports their important functional role in obesity...
  34. ncbi request reprint On the role of placental growth factor in murine adipogenesis
    Valerie Christiaens
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, Belgium
    Thromb Res 120:399-405. 2007
    ..Thus, in murine model systems, PlGF does not seem to play an important role in early adipogenesis...
  35. doi request reprint Short-term ethinyl estradiol treatment suppresses inferior caval vein thrombosis in obese mice
    Audrey C A Cleuren
    Center for Molecular and Vascular Biology, KU Leuven, 3000 Leuven, Belgium
    Thromb Haemost 102:993-1000. 2009
    ....
  36. ncbi request reprint Impairment of adipose tissue development by hypoxia is not mediated by plasminogen activator inhibitor-1
    Fabrizio Semeraro
    Center for Molecular and Vascular Biology, KU Leuven, Belgium
    Thromb Haemost 95:174-81. 2006
    ..In conclusion, impairment of in vivo adipose tissue development and in vitro differentiation of preadipocytes by hypoxia is not mediated by PAI-1...
  37. ncbi request reprint Effect of plasminogen activator inhibitor-1 on adipogenesis in vivo
    Ilse Scroyen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49, Box 911, B 3000 Leuven, Belgium
    Thromb Haemost 101:388-93. 2009
    ..Whereas local expression resulted in larger fat pads, systemic overexpression had no effect on de novo adipogenesis, although angiogenesis appeared to be impaired...
  38. ncbi request reprint Role of Gas-6 in adipogenesis and nutritionally induced adipose tissue development in mice
    Erik Maquoi
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 25:1002-7. 2005
    ..A potential role of growth arrest-specific gene 6 (Gas-6) in energy storage in adipose tissue was investigated in murine models of obesity. Gas-6 is a ligand for the Axl, C-Mer, and Sky family of tyrosine kinase receptors...
  39. doi request reprint Effect of premature aging on murine adipose tissue
    Bianca Hemmeryckx
    Center for Molecular and Vascular Biology, Katholieke Universiteit Leuven, 3000 Leuven, Belgium
    Exp Gerontol 47:256-62. 2012
    ..Thus, premature aging in Bmal1(-/-) mice kept on normal chow was associated with reduced adiposity...
  40. ncbi request reprint Effect of matrix metalloproteinase inhibition on adipose tissue development
    Diego Demeulemeester
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Biochem Biophys Res Commun 329:105-10. 2005
    ..Possibly, enhanced MMP expression to some extent counteracts the in vivo effect of the inhibitor in adipose tissue...
  41. doi request reprint Obesity promotes injury induced femoral artery thrombosis in mice
    Nobuo Nagai
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    Thromb Res 122:549-55. 2008
    ..80; p<0.0001) or Factor V levels (r=0.65; p=0.003). Thus, this injury induced femoral artery thrombosis model in mice establishes experimentally a correlation between obesity and prothrombotic tendency...
  42. ncbi request reprint Mouse carotid artery ligation induces platelet-leukocyte-dependent luminal fibrin, required for neointima development
    T Kawasaki
    Center for Molecular and Vascular Biology, University of Leuven, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    Circ Res 88:159-66. 2001
    ..Delayed coagulation then results in formation of a fibrin matrix, which is used by smooth muscle cells to migrate into the lumen...
  43. ncbi request reprint Stromelysin-1 (MMP-3)-independent gelatinase expression and activation in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Blood 91:2045-53. 1998
    ..These data thus indicate that proMMP-2 activation may occur via a plasmin- and MMP-3-independent mechanism, whereas plasmin can directly activate proMMP-9 via a MMP-3-independent mechanism...
  44. doi request reprint Role of proteolysis in development of murine adipose tissue
    Valerie Christiaens
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49, Leuven, Belgium
    Thromb Haemost 99:290-4. 2008
    ..These studies support a role of both systems in adipogenesis and obesity, and suggest that modulation of proteolytic activity may affect development of adipose tissue...
  45. ncbi request reprint Role of the fibrinolytic and matrix metalloproteinase systems in development of adipose tissue
    Valerie Christiaens
    Center for Molecular and Vascular Biology, KULeuven, Leuven, Belgium
    Arch Physiol Biochem 112:254-9. 2006
    ..These studies support a role of both systems in adipogenesis and obesity; the role of specific members of these families, however, remains to be determined...
  46. ncbi request reprint Overexpression of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in mice does not affect adipogenesis or adipose tissue development
    Diego Demeulemeester
    Center for Molecular and Vascular Biology, K U Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000, Leuven, Belgium
    Thromb Haemost 95:1019-24. 2006
    ..Thus, overexpression of hTIMP-1 in mice had no significant effect on ongoing adipogenesis or adipose tissue development, although the blood vessel size in adipose tissues was reduced...
  47. doi request reprint Growth arrest-specific protein 6 receptor antagonism impairs adipocyte differentiation and adipose tissue development in mice
    H Roger Lijnen
    Center for Molecular and Vascular Biology, Katholieke Universiteit Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49, Box 911, B 3000 Leuven, Belgium
    J Pharmacol Exp Ther 337:457-64. 2011
    ..Thus, affecting GAS6 signaling through receptor antagonism using a low-molecular-weight Axl antagonist impairs adipocyte differentiation and reduces adipose tissue development in a murine model of nutritionally induced obesity...
  48. ncbi request reprint Characterization of the murine plasma fibrinolytic system
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Eur J Biochem 224:863-71. 1994
    ....
  49. ncbi request reprint Role of plasminogen system components in focal cerebral ischemic infarction: a gene targeting and gene transfer study in mice
    N Nagai
    Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Center for Molecular and Vascular Biology, KU Leuven, Belgium
    Circulation 99:2440-4. 1999
    ....
  50. doi request reprint Caloric restriction improves coagulation and inflammation profile in obese mice
    H Roger Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    Thromb Res 129:74-9. 2012
    ..Thus, caloric restriction and drastic weight loss in obese mice is associated with improved plasma coagulation profile and with reduced oxidative stress and inflammation in the adipose tissues...
  51. ncbi request reprint Expression of aggrecan(ases) during murine preadipocyte differentiation and adipose tissue development
    Gabor Voros
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N, Herestraat 49, Leuven, Belgium
    Biochim Biophys Acta 1760:1837-44. 2006
    ..Proteoglycans in the extracellular matrix of adipose tissue, such as aggrecan, may contribute to the regulation of lipid uptake and obesity in mice...
  52. ncbi request reprint Effect of tissue inhibitor of matrix metalloproteinases-1 on in vitro and in vivo adipocyte differentiation
    Ilse Scroyen
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    Thromb Res 124:578-83. 2009
    ..Thus, local hTIMP-1 overexpression did not significantly affect early stages of adipogenesis as evaluated from the extent of in vitro adipocyte differentiation or of in vivo de novo adipose tissue formation...
  53. ncbi request reprint Impaired adipose tissue development in mice with inactivation of placental growth factor function
    H Roger Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Box 911, Herestraat 49, B 3000 Leuven, Belgium
    Diabetes 55:2698-704. 2006
    ..Thus, in murine models of diet-induced obesity, inactivation of PlGF impairs adipose tissue development, at least in part as a result of reduced angiogenesis...
  54. ncbi request reprint Differential expression of plasminogen activator inhibitor-1, tumor necrosis factor-alpha, TNF-alpha converting enzyme and ADAMTS family members in murine fat territories
    Gabor Voros
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg O and N, Herestraat 49, B 3000 Louvain, Belgium
    Biochim Biophys Acta 1625:36-42. 2003
    ..Several ADAM and ADAMTS family members are expressed in adipose tissue and during differentiation of preadipocytes. Modulation of their expression upon development of obesity is adipose tissue-dependent...
  55. ncbi request reprint Vascular release of plasminogen activator inhibitor-1 impairs fibrinolysis during acute arterial thrombosis in mice
    T Kawasaki
    Center for Molecular and Vascular Biology, University of Leuven, and Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    Blood 96:153-60. 2000
    ..Hence, during acute thrombosis, fibrinolysis is efficiently prevented by plasma alpha(2)-AP, but also by vascular PAI-1, locally released into the circulation after endothelial injury...
  56. doi request reprint CD36 promotes adipocyte differentiation and adipogenesis
    Valerie Christiaens
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    Biochim Biophys Acta 1820:949-56. 2012
    ..CD36 is a membrane glycoprotein, contributing to the pathogenesis of metabolic disorders, like obesity, which has become a major health concern worldwide...
  57. doi request reprint Axl deficiency does not affect adipogenesis or adipose tissue development
    Ilse Scroyen
    Center for Molecular and Vascular Biology, Leuven, Belgium
    Obesity (Silver Spring) 20:1168-73. 2012
    ..Glucose and insulin tolerance tests (ITT) in Axl(-/-) and Axl(+/+) mice did not reveal significant differences in glucose homeostasis. Thus, Axl deficiency had no significant effect on adipogenesis in vitro or in vivo...
  58. ncbi request reprint Rofecoxib impairs adipose tissue development in a murine model of nutritionally induced obesity
    H Roger Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Leuven, Belgium
    Thromb Haemost 100:338-42. 2008
    ..Thus, in a nutritionally induced murine obesity model, oral administration of Vioxx, as compared to placebo, resulted in reduced adipose tissue development, associated with lower feeding efficiency and smaller adipocytes...
  59. ncbi request reprint Age-associated adaptations in murine adipose tissues
    Bianca Hemmeryckx
    Center for Molecular and Vascular Biology, Katholieke Universiteit Leuven, Leuven, Belgium
    Endocr J 57:925-30. 2010
    ..Thus, ageing of C57Bl/6 male mice is associated with a biphasic pattern (increase up to 12 months followed by a decrease up to 24 months) of body weight, SC and GON fat mass, adipocyte and blood vessel size...
  60. ncbi request reprint Factor V Leiden mutation is associated with enhanced arterial thrombotic tendency in lean but not in obese mice
    Nobuo Nagai
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49, Box 911, 3000 Leuven, Belgium
    Thromb Haemost 98:858-63. 2007
    ....
  61. ncbi request reprint Obesity and vascular risk
    H R Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, 3000 Leuven, Belgium
    Hamostaseologie 29:44-5. 2009
    ..Venous or arterial thrombosis models in obese mice confirm a prothrombotic risk associated with obesity...
  62. doi request reprint Matrix metalloproteinase inhibition affects adipose tissue mass in obese mice
    Matthias Van Hul
    Center for Molecular and Vascular Biology, Katholieke Universiteit Leuven, Leuven, Belgium
    Clin Exp Pharmacol Physiol 39:544-50. 2012
    ..001). 5. Thus, the relatively gelatinase-specific MMP inhibitor tolylsam has the potential to affect fat tissue growth in obese mice...
  63. ncbi request reprint What has been learned about the cardiovascular effects of matrix metalloproteinases from mouse models?
    Stefan Janssens
    Department of Cardiology, Center for Transgene Technology and Gene Therapy, Flemish Interuniversity Institute for Biotechnology, University of Leuven, Leuven, Belgium
    Cardiovasc Res 69:585-94. 2006
    ....
  64. ncbi request reprint Biological effects of combined inactivation of plasminogen activator and plasminogen activator inhibitor-1 gene function in mice
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 74:1126-31. 1995
    ....
  65. pmc Plasminogen activation: a mediator of vascular smooth muscle cell apoptosis in atherosclerotic plaques
    P Rossignol
    Center for Molecular and Vascular Biology, K U Leuven, Belgium
    J Thromb Haemost 4:664-70. 2006
    ..Plasmin can be generated in atherosclerotic plaques and recent in vitro data suggest that plasminogen activation may trigger vascular smooth muscle cell (VSMC) apoptosis...
  66. ncbi request reprint Critical role of glutamic acid 202 in the enzymatic activity of stromelysin-1 (MMP-3)
    B Arza
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Eur J Biochem 268:826-31. 2001
    ..Taken together, these data suggest that Glu202 plays a crucial role in the enzymatic activity of MMP-3...
  67. ncbi request reprint No functional role of plasminogen activator inhibitor-1 in murine adipogenesis or adipocyte differentiation
    I Scroyen
    Department of Molecular and Cellular Medicine, Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    J Thromb Haemost 5:139-45. 2007
    ..To substantiate a potential role of plasminogen activator inhibitor-1 (PAI-1) in adipogenesis, we have studied its effects on in vitro adipocyte differentiation and on in vivo adipose tissue formation...
  68. ncbi request reprint Modulation of adipose tissue expression of murine matrix metalloproteinases and their tissue inhibitors with obesity
    Erik Maquoi
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Diabetes 51:1093-101. 2002
    ..Moreover, this in vitro adipogenesis was reduced in the presence of a synthetic MMP inhibitor. Thus, the adipose tissue expresses a large array of MMPs and TIMPs, which modulate adipocyte differentiation...
  69. ncbi request reprint Angiogenesis and obesity
    H Roger Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49, Box 911, 3000 Leuven, Belgium
    Cardiovasc Res 78:286-93. 2008
    ..Modulation of angiogenesis and of proteolytic systems may have the potential to impair adipose tissue development...
  70. ncbi request reprint Matrix metalloproteinase inhibition impairs murine adipose tissue development independently of leptin
    Matthias Van Hul
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    Endocr J 58:101-7. 2011
    ..This indicates that MMP inhibition may impair adipose tissue development independently of leptin...
  71. ncbi request reprint Influence of membrane-bound tumor necrosis factor (TNF)-alpha on obesity and glucose metabolism
    G Voros
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    J Thromb Haemost 2:507-13. 2004
    ..To investigate the influence of transmembrane tumor necrosis factor (TNF)-alpha on adipose tissue development and insulin-mediated glucose metabolism...
  72. ncbi request reprint Characterization of a recombinant chimeric plasminogen activator composed of a fibrin fragment-D-dimer-specific humanized monoclonal antibody and a truncated single-chain urokinase
    A M Vandamme
    Center for Thrombosis and Vascular Research, University of Leuven, Belgium
    Eur J Biochem 205:139-46. 1992
    ..The chimera has intact antigen-binding capacity, u-PA enzymatic activity and a significantly increased fibrinolytic potency in a plasma medium in vitro...
  73. doi request reprint Role of fibrinolysis in obesity and thrombosis
    H R Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Belgium
    Thromb Res 123:S46-9. 2009
    ..Studies using venous or arterial thrombosis models in obese mice, with impaired fibrinolytic activity, confirm a prothrombotic risk associated with obesity...
  74. ncbi request reprint Characterization of a chimeric plasminogen activator consisting of a single-chain Fv fragment derived from a fibrin fragment D-dimer-specific antibody and a truncated single-chain urokinase
    P Holvoet
    Center for Thrombosis and Vascular Research, University of Leuven, Belgium
    J Biol Chem 266:19717-24. 1991
    ..5-fold increase of the potency of the two-chain form...
  75. ncbi request reprint Restoration of thrombolytic potential in plasminogen-deficient mice by bolus administration of plasminogen
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Blood 88:870-6. 1996
    ..Thus, restoration of normal plasminogen levels in Plg-/- mice normalized the thrombolytic potential toward experimentally induced pulmonary emboli, and resulted in removal of endogenous fibrin deposits within 24 hours...
  76. doi request reprint Age-associated pro-inflammatory adaptations of the mouse thoracic aorta
    Bianca Hemmeryckx
    H R Lijnen, Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, CDG, Herestraat 49, Box 911, 3000 Leuven, Belgium, Tel 32 16 345771, Fax 32 16 345990, E mail
    Thromb Haemost 110:785-94. 2013
    ..In conclusion, a reduction in endothelial cell-mediated vasodilation in aged thoracic aortas of C57BL/6J mice was accompanied by a shift towards a pro-inflammatory state of the endothelium. ..
  77. doi request reprint Functional interactions between pancreatic beta cells and (pre)adipocytes
    Valerie Christiaens
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, Leuven, Belgium
    Endocrine 38:118-26. 2010
    ..Over 9 days culture, (pre)adipocytes did not directly and irreversibly affect beta cell functions...
  78. doi request reprint The tissue-type plasminogen activator story
    D Collen
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49 Box 911, B 3000 Leuven, Belgium
    Arterioscler Thromb Vasc Biol 29:1151-5. 2009
    ....
  79. ncbi request reprint Reduced atherosclerotic plaque but enhanced aneurysm formation in mice with inactivation of the tissue inhibitor of metalloproteinase-1 (TIMP-1) gene
    J Silence
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Circ Res 90:897-903. 2002
    ..These data suggest that enhanced MMP activity, as a result of TIMP-1 deficiency, contributes to a reduction of atherosclerotic plaque size but promotes aneurysm formation...
  80. ncbi request reprint Matrix metalloproteinase deficiencies do not impair cell-associated fibrinolytic activity
    F Ugwu
    Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O and N, Herestraat 49, B 3000, Leuven, Belgium
    Thromb Res 102:61-9. 2001
    ....
  81. ncbi request reprint Adipocyte hypertrophy in stromelysin-3 deficient mice with nutritionally induced obesity
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 87:530-5. 2002
    ..As the main biological function of MMP-11 remains unknown, it is not clear whether the adipocyte hypertrophy in MMP-11-/- adipose tissue is directly related to the deficiency or to other pathways affected by MMP-11...
  82. ncbi request reprint Plasminogen activator inhibitor-1 contributes to the deleterious effect of obesity on the outcome of thrombotic ischemic stroke in mice
    N Nagai
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    J Thromb Haemost 5:1726-31. 2007
    ..Adipose tissue is an important source of plasminogen activator inhibitor-1 (PAI-1), the main inhibitor of plasminogen activation...
  83. doi request reprint Stromelysin-2 (MMP-10) deficiency does not affect adipose tissue formation in a mouse model of nutritionally induced obesity
    H R Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, B 3000 Leuven, Belgium
    Biochem Biophys Res Commun 389:378-81. 2009
    ..These data indicate that MMP-10 does not significantly contribute to adipose tissue development and associated angiogenesis in a mouse model of nutritionally induced obesity...
  84. ncbi request reprint Effects of plasminogen activator inhibitor-1 on ischemic brain injury in permanent and thrombotic middle cerebral artery occlusion models in mice
    N Nagai
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    J Thromb Haemost 3:1379-84. 2005
    ..Here, the effect of plasminogen activator inhibitor-1 (PAI-1), an endogenous inhibitor of t-PA, on ischemic infarct volume was studied...
  85. doi request reprint Progression of the prothrombotic state in aging Bmal1-deficient mice
    Bianca Hemmeryckx
    Center for Molecular and Vascular Biology, Katholieke Universiteit Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 31:2552-9. 2011
    ..The goal of this study was to examine the functional relationship between aging endothelium and thrombogenicity in a mouse model of premature aging...
  86. ncbi request reprint Thrombolytic agents
    Desire Collen
    Center for Molecular and Vascular Biology, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Thromb Haemost 93:627-30. 2005
    ..Following a decade of clinical investigation t-PA and variants thereof are routinely used for treatment of patients with thromboembolic disease...
  87. ncbi request reprint Arginine 719 in human plasminogen mediates formation of the staphylokinase:plasmin activator complex
    L Jespers
    Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, and Center for Molecular and Vascular Biology, KULeuven, Leuven, Belgium
    Biochemistry 37:6380-6. 1998
    ..M., Marshall, J. M., Raper, R. H., Gilbert, R. J., and Ponting, C. P. (1994) Biochemistry 33, 12042-12047.], suggesting that both bacterial cofactors may share common structural and/or mechanistic aspects for plasminogen activation...
  88. ncbi request reprint Recombinant staphylokinase variants with altered immunoreactivity. I: Construction and characterization
    D Collen
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Circulation 94:197-206. 1996
    ..Although reduced immunogenicity of heterologous proteinaceous drugs by protein engineering has not previously been reported, an attempt was made to achieve this in staphylokinase by site-specific mutagenesis...
  89. ncbi request reprint Enhanced fibrinolytic potential in mice with combined homozygous deficiency of alpha2-antiplasmin and PAI-1
    M Dewerchin
    Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    Thromb Haemost 86:640-6. 2001
    ..Taken together, these data indicate that the higher endogenous fibrinolytic capacity observed in mice with combined deficiency is mainly due to the lack of alpha2-AP and suggest a less important role for PAI-1...
  90. ncbi request reprint Tissue-type plasminogen activator: a historical perspective and personal account
    D Collen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    J Thromb Haemost 2:541-6. 2004
    ..Genetically modified variants of t-PA have been developed for bolus administration in patients with AMI...
  91. ncbi request reprint Functional role of the fibrinolytic system in development of adipose tissue
    H R Lijnen
    Center for Molecular and Vascular Biology, Faculty of Medicine, Campus Gasthuisberg, Katholieke Universiteit Leuven, Leuven
    Verh K Acad Geneeskd Belg 71:101-13. 2009
    ..These studies thus support a role for fibrinolytic activity and suggest that its modulation may allow to affect development of adipose tissue...
  92. doi request reprint A functional role of gelatinase A in the development of nutritionally induced obesity in mice
    M Van Hul
    Centre for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    J Thromb Haemost 6:1198-206. 2008
    ..Development of adipose tissue is a complex process involving adipogenesis, angiogenesis and proteolytic remodeling of the extracellular matrix. The matrix metalloproteinase (MMP) system plays an important role in these processes...
  93. ncbi request reprint Characterization and targeting of the murine alpha2-antiplasmin gene
    K Okada
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Thromb Haemost 78:1104-10. 1997
    ..These targeted clones will be used to generate alpha2-AP deficient mice...
  94. ncbi request reprint Alpha2-antiplasmin gene deficiency in mice is associated with enhanced fibrinolytic potential without overt bleeding
    H R Lijnen
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Blood 93:2274-81. 1999
    ..005). Thus, alpha2-AP-/- mice develop and reproduce normally; they have an enhanced endogenous fibrinolytic capacity without overt bleeding...
  95. ncbi request reprint Stromelysin-1 (MMP-3) is critical for intracranial bleeding after t-PA treatment of stroke in mice
    Y Suzuki
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    J Thromb Haemost 5:1732-9. 2007
    ..Matrix metalloproteinases (MMPs), which can be activated through the plasminogen/plasmin system, may contribute to ICB after ischemic stroke...
  96. ncbi request reprint Thrombolytic efficacy of recombinant human microplasmin in a canine model of copper coil-induced coronary artery thrombosis
    Christophe Dommke
    Department of Cardiology, University Hospital Gasthuisberg, Leuven, Belgium
    J Thromb Thrombolysis 30:46-54. 2010
    ..Intracoronay administration of microplasmin effectively lysed coronary thrombosis...
  97. ncbi request reprint Deficiency of plasminogen activator inhibitor-2 impairs nutritionally induced murine adipose tissue development
    H R Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium
    J Thromb Haemost 5:2259-65. 2007
    ..No information is available, however, on a potential role of plasminogen activator inhibitor-2 (PAI-2) in obesity...
  98. doi request reprint Effect of fumagillin on adipocyte differentiation and adipogenesis
    I Scroyen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N 1, Herestraat 49, Box 911, 3000 Leuven, Belgium
    Biochim Biophys Acta 1800:425-9. 2010
    ..Inhibition of angiogenesis may impair adipose tissue development...
  99. ncbi request reprint Inhibition of vascular endothelial growth factor receptor tyrosine kinases impairs adipose tissue development in mouse models of obesity
    H R Lijnen
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N1, Herestraat 49, Box 911, 3000 Leuven, Belgium
    Biochim Biophys Acta 1770:1369-73. 2007
    ..Thus, in nutritionally induced murine obesity models, oral administration of the VEGFR tyrosine kinases inhibitor PTK787 resulted in reduced adipose tissue development...
  100. doi request reprint Gelatinase B (MMP-9) deficiency does not affect murine adipose tissue development
    Matthias Van Hul
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, Herestraat 49, Leuven, Belgium
    Thromb Haemost 104:165-71. 2010
    ..0001). However, MMP-9 does not seem to play a major role in adipose tissue development in murine models of diet-induced obesity...
  101. ncbi request reprint Persistence of atherosclerotic plaque but reduced aneurysm formation in mice with stromelysin-1 (MMP-3) gene inactivation
    J Silence
    Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
    Arterioscler Thromb Vasc Biol 21:1440-5. 2001
    ..MMP-3 activity may then contribute to a reduction of plaque size, possibly by degradation of matrix components, and promote aneurysm formation by degradation of the elastica lamina...