Jun ichi Hotta

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:16132-41. 2007
  2. doi Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores
    Jun ichi Hotta
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    J Am Chem Soc 132:5021-3. 2010
  3. ncbi A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants
    Cristina Flors
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:13970-7. 2007
  4. ncbi Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Photochem Photobiol Sci 5:567-76. 2006
  5. pmc Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy
    Peter Dedecker
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 91:L45-7. 2006
  6. ncbi 3D nanoscopy: bringing biological nanostructures into sharp focus
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Angew Chem Int Ed Engl 46:8330-2. 2007
  7. ncbi Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor: is cis-trans isomerization involved?
    Davey C Loos
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 128:6270-1. 2006
  8. doi Data storage based on photochromic and photoconvertible fluorescent proteins
    Virgile Adam
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Biotechnol 149:289-98. 2010
  9. pmc Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters
    Yasuko Antoku
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium
    Photochem Photobiol Sci 9:716-21. 2010
  10. pmc Visualization of membrane rafts using a perylene monoimide derivative and fluorescence lifetime imaging
    Anca Margineanu
    Laboratory of Photochemistry and Spectroscopy, Katholieke Universiteit Leuven, Leuven, Belgium
    Biophys J 93:2877-91. 2007

Collaborators

Detail Information

Publications10

  1. ncbi Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:16132-41. 2007
    ..We experimentally demonstrate the possibility of obtaining increased resolution by making use of the efficient and thermally stable Dronpa photoswitching, using equipment that is commonly available...
  2. doi Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores
    Jun ichi Hotta
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    J Am Chem Soc 132:5021-3. 2010
    ..Thus, assessment of the excited-state absorption band provides a rational means of dye selection and determination of the optimal wavelength for STED...
  3. ncbi A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants
    Cristina Flors
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:13970-7. 2007
    ..We provide a new approach to achieve fast PALM by introducing simultaneous two-color stroboscopic illumination...
  4. ncbi Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Photochem Photobiol Sci 5:567-76. 2006
    ..Our results demonstrate that Dronpa holds its outstanding photoswitching properties, based on a photo-induced protonation/deprotonation process, even at the single-molecule level...
  5. pmc Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy
    Peter Dedecker
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 91:L45-7. 2006
    ....
  6. ncbi 3D nanoscopy: bringing biological nanostructures into sharp focus
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Angew Chem Int Ed Engl 46:8330-2. 2007
  7. ncbi Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor: is cis-trans isomerization involved?
    Davey C Loos
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 128:6270-1. 2006
    ..These observations are tentatively interpreted as trans-to-cis isomerization of the chromophore taking place upon irradiation together with the opening of new, nonradiative pathways...
  8. doi Data storage based on photochromic and photoconvertible fluorescent proteins
    Virgile Adam
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Biotechnol 149:289-98. 2010
    ..Hence, this contribution highlights several data storage schemes based on the remarkable properties of PCFPs/RSFPs...
  9. pmc Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters
    Yasuko Antoku
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium
    Photochem Photobiol Sci 9:716-21. 2010
    ....
  10. pmc Visualization of membrane rafts using a perylene monoimide derivative and fluorescence lifetime imaging
    Anca Margineanu
    Laboratory of Photochemistry and Spectroscopy, Katholieke Universiteit Leuven, Leuven, Belgium
    Biophys J 93:2877-91. 2007
    ..For other fluorophores with QY<1, the suggested mechanism could also contribute, in addition to effects reducing the nonradiative decay pathways, to an increase of the fluorescence decay time in the Lo phase...