Johan Hofkens

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Triplet states as non-radiative traps in multichromophoric entities: single molecule spectroscopy of an artificial and natural antenna system
    J Hofkens
    Department of Organic Chemistry, Katholieke Universiteit Leuven, Belgium
    Spectrochim Acta A Mol Biomol Spectrosc 57:2093-107. 2001
  2. pmc Revealing competitive Forster-type resonance energy-transfer pathways in single bichromophoric molecules
    Johan Hofkens
    Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    Proc Natl Acad Sci U S A 100:13146-51. 2003
  3. doi request reprint Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications
    Virgile Adam
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Chem Biol 18:1241-51. 2011
  4. ncbi request reprint Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:16132-41. 2007
  5. ncbi request reprint A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants
    Cristina Flors
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:13970-7. 2007
  6. ncbi request reprint Revealing the excited-state dynamics of the fluorescent protein Dendra2
    Eduard Fron
    Division of Molecular Imaging and Photonics, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Phys Chem B 117:2300-13. 2013
  7. ncbi request reprint Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Photochem Photobiol Sci 5:567-76. 2006
  8. doi request reprint Energy transfer pathways in a rylene-based Triad
    Eduard Fron
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Chemphyschem 12:595-608. 2011
  9. doi request reprint Characterization of fluorescence in heat-treated silver-exchanged zeolites
    Gert De Cremer
    Department of Microbial and Molecular Systems, Centre for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B 3001 Leuven, Belgium
    J Am Chem Soc 131:3049-56. 2009
  10. pmc Single-molecule fluorescence spectroscopy in (bio)catalysis
    Maarten B J Roeffaers
    Department of Microbial and Molecular Systems, Centre for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B 3001 Leuven, Belgium
    Proc Natl Acad Sci U S A 104:12603-9. 2007

Collaborators

Detail Information

Publications67

  1. ncbi request reprint Triplet states as non-radiative traps in multichromophoric entities: single molecule spectroscopy of an artificial and natural antenna system
    J Hofkens
    Department of Organic Chemistry, Katholieke Universiteit Leuven, Belgium
    Spectrochim Acta A Mol Biomol Spectrosc 57:2093-107. 2001
    ....
  2. pmc Revealing competitive Forster-type resonance energy-transfer pathways in single bichromophoric molecules
    Johan Hofkens
    Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    Proc Natl Acad Sci U S A 100:13146-51. 2003
    ..Here we report selectively visualization of different energy-transfer pathways taking place between identical fluorophores in individual bichromophoric molecules...
  3. doi request reprint Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications
    Virgile Adam
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Chem Biol 18:1241-51. 2011
    ..We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic properties that make it ideal for advanced fluorescence-based imaging applications...
  4. ncbi request reprint Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:16132-41. 2007
    ..We experimentally demonstrate the possibility of obtaining increased resolution by making use of the efficient and thermally stable Dronpa photoswitching, using equipment that is commonly available...
  5. ncbi request reprint A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants
    Cristina Flors
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:13970-7. 2007
    ..We provide a new approach to achieve fast PALM by introducing simultaneous two-color stroboscopic illumination...
  6. ncbi request reprint Revealing the excited-state dynamics of the fluorescent protein Dendra2
    Eduard Fron
    Division of Molecular Imaging and Photonics, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Phys Chem B 117:2300-13. 2013
    ....
  7. ncbi request reprint Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Photochem Photobiol Sci 5:567-76. 2006
    ..Our results demonstrate that Dronpa holds its outstanding photoswitching properties, based on a photo-induced protonation/deprotonation process, even at the single-molecule level...
  8. doi request reprint Energy transfer pathways in a rylene-based Triad
    Eduard Fron
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Chemphyschem 12:595-608. 2011
    ....
  9. doi request reprint Characterization of fluorescence in heat-treated silver-exchanged zeolites
    Gert De Cremer
    Department of Microbial and Molecular Systems, Centre for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B 3001 Leuven, Belgium
    J Am Chem Soc 131:3049-56. 2009
    ..This new class of photostable luminescent particles with tunable emission colors offers interesting perspectives for various applications such as biocompatible labels for intracellular imaging...
  10. pmc Single-molecule fluorescence spectroscopy in (bio)catalysis
    Maarten B J Roeffaers
    Department of Microbial and Molecular Systems, Centre for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B 3001 Leuven, Belgium
    Proc Natl Acad Sci U S A 104:12603-9. 2007
    ..Future challenges comprising the integration of other techniques, such as diffraction, scanning probe, or vibrational methods in single-molecule fluorescence spectroscopy are suggested...
  11. doi request reprint Time-resolved single molecule fluorescence spectroscopy of an α-chymotrypsin catalyzed reaction
    Tatyana G Terentyeva
    Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium
    J Phys Chem B 117:1252-60. 2013
    ..Our results clearly show the power of using lifetime-resolved measurements for investigating enzymatic reactions at the single molecule level...
  12. pmc Visualization of membrane rafts using a perylene monoimide derivative and fluorescence lifetime imaging
    Anca Margineanu
    Laboratory of Photochemistry and Spectroscopy, Katholieke Universiteit Leuven, Leuven, Belgium
    Biophys J 93:2877-91. 2007
    ..For other fluorophores with QY<1, the suggested mechanism could also contribute, in addition to effects reducing the nonradiative decay pathways, to an increase of the fluorescence decay time in the Lo phase...
  13. ncbi request reprint Dynamic disorder and stepwise deactivation in a chymotrypsin catalyzed hydrolysis reaction
    Gert De Cremer
    Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B 3001 Leuven, Belgium
    J Am Chem Soc 129:15458-9. 2007
  14. ncbi request reprint Ultrafast excited-state dynamics of the photoswitchable protein Dronpa
    Eduard Fron
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:4870-1. 2007
  15. pmc Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy
    Peter Dedecker
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 91:L45-7. 2006
    ....
  16. pmc Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters
    Yasuko Antoku
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium
    Photochem Photobiol Sci 9:716-21. 2010
    ....
  17. pmc Dark states in monomeric red fluorescent proteins studied by fluorescence correlation and single molecule spectroscopy
    Jelle Hendrix
    Laboratory of Biomolecular Dynamics, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 94:4103-13. 2008
    ..We apply dual-color fluorescence correlation spectroscopy inside live cells to prove that this flickering can seriously hamper cellular measurements if the timescales of the flickering and diffusion are not well separated...
  18. ncbi request reprint Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 125:8446-7. 2003
    ..Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level...
  19. ncbi request reprint Origin of simultaneous donor-acceptor emission in single molecules of peryleneimide-terrylenediimide labeled polyphenylene dendrimers
    Sergey M Melnikov
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Phys Chem B 111:708-19. 2007
    ..Two-color defocused wide-field imaging showed that the two-color emission goes hand in hand with an unfavorable orientation between one of the donors and the acceptor chromophore...
  20. doi request reprint Second-harmonic generation in GFP-like proteins
    Inge Asselberghs
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200D and F, B 3001 Leuven, Belgium
    J Am Chem Soc 130:15713-9. 2008
    ..An accurate value of the first hyperpolarizability is also essential for any qualitative analysis of the nonlinear images...
  21. ncbi request reprint 3D nanoscopy: bringing biological nanostructures into sharp focus
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Angew Chem Int Ed Engl 46:8330-2. 2007
  22. ncbi request reprint Excited state dynamics of the photoconvertible fluorescent protein Kaede revealed by ultrafast spectroscopy
    Eduard Fron
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Photochem Photobiol Sci 13:867-74. 2014
    ..FRET between red neutral and red anionic forms occurs within the tetramer with time constants of 13.4 ps and 210 ps. In contrast to literature proposals no ESPT was observed. ..
  23. doi request reprint Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa
    Ngan Nguyen Bich
    Biomolecular Architecture, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven Heverlee, Belgium
    Acta Crystallogr D Biol Crystallogr 68:1653-9. 2012
    ..It is proposed that the slower off-switching rate in PDM1-4 is caused by a decrease in the potential flexibility of certain β-strands caused by oligomerization along the AC interface...
  24. doi request reprint Photocatalytic growth of dendritic silver nanostructures as SERS substrates
    Katrien G M Laurier
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001, Heverlee, Belgium
    Chem Commun (Camb) 48:1559-61. 2012
    ..These self-organised structures show an excellent Raman enhancement enabling the detection of analytes from dilute solutions by surface-enhanced Raman spectroscopy...
  25. pmc Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Proc Natl Acad Sci U S A 102:9511-6. 2005
    ..The fast response of Dronpa to light holds great promise for following fast diffusion or transport of signaling molecules in live cells...
  26. doi request reprint Fluorescence-based analysis of enzymes at the single-molecule level
    Kerstin Blank
    Katholieke Universiteit Leuven, Department of Chemistry, Belgium
    Biotechnol J 4:465-79. 2009
    ....
  27. doi request reprint Photoactivation of silver-exchanged zeolite A
    Gert De Cremer
    Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, 3001 Heverlee, Belgium
    Angew Chem Int Ed Engl 47:2813-6. 2008
  28. doi request reprint Exploration of single molecule events in a haloperoxidase and its biomimic: localization of halogenation activity
    Virginia Martínez Martínez
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001 Leuven, Belgium
    J Am Chem Soc 130:13192-3. 2008
    ....
  29. pmc Super-resolution optical DNA Mapping via DNA methyltransferase-directed click chemistry
    Charlotte Vranken
    Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium, Life Science Technologies, IMEC, Kapeldreef 75, 3001 Heverlee, Belgium and Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark
    Nucleic Acids Res 42:e50. 2014
    ..Three of 11 enzymes transalkylate DNA with the cofactor we tested (a readily prepared s-adenosyl-l-methionine analogue). ..
  30. ncbi request reprint Green-to-Red Photoconvertible Dronpa Mutant for Multimodal Super-resolution Fluorescence Microscopy
    Benjamien Moeyaert
    Department of Chemistry, KU Leuven, Celestijnenlaan 200F, bus 2404, 3001 Heverlee, Belgium
    ACS Nano 8:1664-73. 2014
    ..We herein show that, despite its tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by sequentially imaging a given sample using both super-resolution fluctuation imaging and localization microscopy. ..
  31. doi request reprint Single molecule methods for the study of catalysis: from enzymes to heterogeneous catalysts
    Kris P F Janssen
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    Chem Soc Rev 43:990-1006. 2014
    ..In the second part topics specific to both bio and heterogeneous catalysis will be reviewed in more detail. ..
  32. doi request reprint Dynamic disorder in single-enzyme experiments: facts and artifacts
    Tatyana G Terentyeva
    Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium
    ACS Nano 6:346-54. 2012
    ..When using change point analysis for data of the enzyme α-chymotrypsin, no characteristics of dynamic disorder was found. In light of these results, dynamic disorder might not be a general sign of enzymatic reactions...
  33. doi request reprint Spectroscopic characterization of Venus at the single molecule level
    Charlotte C David
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium
    Photochem Photobiol Sci 11:358-63. 2012
    ..Such long off-times can complicate the analysis of single molecule counting experiments or single-molecule FRET experiments...
  34. doi request reprint Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters
    Tatyana G Terentyeva
    Department of Chemistry, Katholieke Universiteit Leuven, Heverlee, Belgium
    Bioconjug Chem 22:1932-8. 2011
    ..This knowledge about the determinants of the spectroscopic properties of monosubstituted Rhodamine 110 introduces a way for a more systematic development of new fluorogenic protease substrate analogues...
  35. doi request reprint Data storage based on photochromic and photoconvertible fluorescent proteins
    Virgile Adam
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Biotechnol 149:289-98. 2010
    ..Hence, this contribution highlights several data storage schemes based on the remarkable properties of PCFPs/RSFPs...
  36. doi request reprint Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores
    Jun ichi Hotta
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    J Am Chem Soc 132:5021-3. 2010
    ..Thus, assessment of the excited-state absorption band provides a rational means of dye selection and determination of the optimal wavelength for STED...
  37. ncbi request reprint Evidence for the isomerization and decarboxylation in the photoconversion of the red fluorescent protein DsRed
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 127:8977-84. 2005
    ..Our results demonstrate the importance of the chromophore conformation in the ground state on the brightness of the protein as well as the importance of the photon flux to control/avoid the photoconversion process...
  38. ncbi request reprint Non-conjugated, phenyl assisted coupling in through bond electron transfer in a perylenemonoimide-triphenylamine system
    Toby D M Bell
    Department of Chemistry, Katholieke Universiteit Leuven, Insitute for Nanoscale Physics and Chemistry, Celestijnenlaan 200F, 3001, Heverlee, Belgium
    Photochem Photobiol Sci 6:406-15. 2007
    ..The calculations also indicate a strong angle dependence of the total coupling in both isomers. The experimental results are discussed in the context of the high temperature limit of Marcus's theory of non-adiabatic ET...
  39. doi request reprint Linking phospholipase mobility to activity by single-molecule wide-field microscopy
    Susana Rocha
    Division of Molecular and Nano Materials, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium
    Chemphyschem 10:151-61. 2009
    ..The methods introduced herein represent a promising new approach to the study of interfacial/heterogeneous catalysis systems...
  40. doi request reprint Delayed electron-hole pair recombination in iron(III)-oxo metal-organic frameworks
    Katrien G M Laurier
    Department of Chemistry, KU Leuven, Celestijnenlaan 200F, B 3001, Heverlee, Belgium
    Phys Chem Chem Phys 16:5044-7. 2014
    ..Furthermore, photogeneration of long-lived species in MIL-88B(Fe) has been proven via transient absorption spectroscopy...
  41. ncbi request reprint Photoswitchable fluorescent proteins for superresolution fluorescence microscopy circumventing the diffraction limit of light
    Susana Rocha
    Department of Chemistry, KU Leuven, Leuven, Belgium
    Methods Mol Biol 1076:793-812. 2014
    ..Here we introduce a basic protocol of PALM through the visualization of actin bundles with superresolution. ..
  42. doi request reprint X-ray irradiation-induced formation of luminescent silver clusters in nanoporous matrices
    Eduardo Coutiño-Gonzalez
    Department of Chemistry, KU Leuven, Celestijnenlaan 200F, B 3001 Leuven, Belgium
    Chem Commun (Camb) 50:1350-2. 2014
    ..The activated luminescent samples were investigated by employing a combination of stationary and time-resolved spectroscopic techniques. ..
  43. ncbi request reprint Unraveling excited-state dynamics in a polyfluorene-perylenediimide copolymer
    Eduard Fron
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium, Max Planck Institut fur Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany, and Laboratoire de Spectrochimie Infrarouge et Raman UMR 8516 du CNRS, Centre d Etudes et de Recherches Lasers et Applications FR 2416 du CNRS, Universite Lille 1 Sciences et Technologies de Lille, bat C5, 59655 Villeneuve d Ascq cedex, France
    J Phys Chem B 114:1277-86. 2010
    ..The data suggest that a fraction of the absorbed energy is directed, transferred, and used in charge separation, providing a clear view of a multistep mechanism of exciton dissociation into charges...
  44. ncbi request reprint Probing the influence of O2 on photoinduced reversible electron transfer in perylenediimide-triphenylamine-based dendrimers by single-molecule spectroscopy
    Mircea Cotlet
    Laboratory of Photochemistry and Spectroscopy, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Angew Chem Int Ed Engl 43:6116-20. 2004
  45. doi request reprint Iron(III)-based metal-organic frameworks as visible light photocatalysts
    Katrien G M Laurier
    Department of Chemistry, KU Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    J Am Chem Soc 135:14488-91. 2013
    ..For the first time, the remarkable photocatalytic efficiency of such Fe(III)-based MOFs under visible light illumination (350 up to 850 nm) is shown. ..
  46. doi request reprint Three-dimensional visualization of defects formed during the synthesis of metal-organic frameworks: a fluorescence microscopy study
    Rob Ameloot
    Center for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Belgium
    Angew Chem Int Ed Engl 52:401-5. 2013
    ..In contrast to currently available techniques, confocal fluorescence microscopy offers the advantage of three-dimensional imaging at the single-crystal level combined with the sensitivity required to study the start of defect formation...
  47. doi request reprint Towards direct monitoring of discrete events in a catalytic cycle at the single molecule level
    Rob Ameloot
    Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B 3001, Leuven, Belgium
    Photochem Photobiol Sci 8:453-6. 2009
    ..The binding state dependent quenching behaviour of functionalized dyes opens perspectives on directly monitoring binding and dissociation events in a catalytic cycle at the single molecule level...
  48. ncbi request reprint Fluorescent proteins: shine on, you crazy diamond
    Peter Dedecker
    Department of Chemistry, University of Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 135:2387-402. 2013
    ..We pay particular attention to emerging or future research opportunities and unsolved questions...
  49. doi request reprint The Escherichia coli GTPase ObgE modulates hydroxyl radical levels in response to DNA replication fork arrest
    Cyrielle I Kint
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium
    FEBS J 279:3692-704. 2012
    ..Together, these results indicate involvement of hydroxyl radical toxicity in ObgE-mediated protection against replication fork stress...
  50. doi request reprint Excitation polarization sensitivity of plasmon-mediated silver nanotriangle growth on a surface
    Aniruddha Paul
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F 3001 Heverlee, Belgium
    Langmuir 28:8920-5. 2012
    ..Thus, this study demonstrates that light-induced, directional synthesis of nanoparticles on solid substrates is in reach, which is of utmost importance for plasmonic applications...
  51. ncbi request reprint Mapping of surface-enhanced fluorescence on metal nanoparticles using super-resolution photoactivation localization microscopy
    Hongzhen Lin
    Katholieke Universiteit Leuven, Celestijnenlaan 200F 3001 Heverlee, Belgium
    Chemphyschem 13:973-81. 2012
    ..Furthermore, nanostructures with sizes below the diffraction limit can be resolved using this technique. SEF-PALM is established as a powerful tool to study plasmon-mediated phenomena on metal nanostructures...
  52. ncbi request reprint Space- and time-resolved visualization of acid catalysis in ZSM-5 crystals by fluorescence microscopy
    Maarten B J Roeffaers
    Department of Microbial and Molecular Systems, Centre for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, 3001 Leuven, Belgium
    Angew Chem Int Ed Engl 46:1706-9. 2007
  53. doi request reprint Molecular organization of hydrophobic molecules and co-adsorbed water in SBA-15 ordered mesoporous silica material
    Randy Mellaerts
    Centre for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, BE 3001 Heverlee, Belgium
    Phys Chem Chem Phys 13:2706-13. 2011
    ..These insights contribute to the development of practical methods for loading ordered mesoporous silica materials with hydrophobic molecules...
  54. pmc Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
    Davey Loos
    Laboratory of Photochemistry and Spectroscopy, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    Biophys J 87:2598-608. 2004
    ....
  55. doi request reprint Subdiffraction limited, remote excitation of surface enhanced Raman scattering
    James A Hutchison
    Division of Molecular and Nano Materials, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, Heverlee 3001, Belgium
    Nano Lett 9:995-1001. 2009
    ..The ability to transfer SERS excitation over several micrometers, through a structure with a subdiffraction limit diameter, is discussed with respect to potential high-resolution SERS imaging applications...
  56. pmc HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme
    Doortje Borrenberghs
    Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, Flanders, Belgium
    ACS Nano 8:3531-45. 2014
    ..e., other PPIs targeted into the HIV-1, or PPIs targeted into other viruses, can potentially be studied with a similar strategy. ..
  57. ncbi request reprint Excited state dynamics of photoswitchable fluorescent protein Padron
    Eduard Fron
    Molecular Imaging and Photonics, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Phys Chem B 117:16422-7. 2013
    ..Consequently, upon excitation of the formed anionic species a trans-cis isomerization of the chromophore was found to occur with a time constant as fast as 5.2 ps switching the chromophore quantitatively to the bright (anionic) state. ..
  58. pmc Auto-production of biosurfactants reverses the coffee ring effect in a bacterial system
    Wouter Sempels
    Department of Chemistry, KU Leuven, University of Leuven, Celestijnenlaan 200F, B 3001 Leuven, Belgium
    Nat Commun 4:1757. 2013
    ..Moreover, at biologically relevant conditions, intricate time-dependent flows are observed in addition to the vortex regime, which are also effective in reversing the coffee ring effect at even lower surfactant concentrations...
  59. doi request reprint Optical mapping of DNA: single-molecule-based methods for mapping genomes
    Robert K Neely
    Departement Chemie, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    Biopolymers 95:298-311. 2011
    ..And, finally, this review will discuss the recent development of mapping with subdiffraction-limit precision using methyltransferase enzymes to label the DNA with an ultrahigh density...
  60. pmc Resonance energy transfer in a calcium concentration-dependent cameleon protein
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Biophys J 83:3499-506. 2002
    ..1 samples...
  61. ncbi request reprint Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches
    Branko Kolaric
    Katholieke Universiteit Leuven, Department Chemistry and Institute for Nanoscale Physics and Chemistry INPAC, Celestijnenlaan 200F, Heverlee, B 3001, Belgium
    Photochem Photobiol Sci 6:614-8. 2007
    ..On the single molecule level, each DNA-based nanometric construct shows a complete switching. These observations suggest the tendency of the DNA construct to associate at high concentration...
  62. ncbi request reprint Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor: is cis-trans isomerization involved?
    Davey C Loos
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 128:6270-1. 2006
    ..These observations are tentatively interpreted as trans-to-cis isomerization of the chromophore taking place upon irradiation together with the opening of new, nonradiative pathways...
  63. doi request reprint Synthesis and in vitro evaluation of a PDT active BODIPY-NLS conjugate
    Peter Verwilst
    Division of Molecular Design and Synthesis, Department of Chemistry, University of Leuven, Celestijnenlaan 200F, P O Box 2404, 3001 Heverlee, Belgium
    Bioorg Med Chem Lett 23:3204-7. 2013
    ..Cellular co-localization experiments revealed a localization of both dyes in the lysosomes, as well as a partial localization within the ER (for the peptide-bearing BODIPY)...
  64. ncbi request reprint Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy
    Noel Boens
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200f bus 02404, 3001 Heverlee, Belgium
    Anal Chem 79:2137-49. 2007
    ..Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given...
  65. doi request reprint Water-soluble monofunctional perylene and terrylene dyes: powerful labels for single-enzyme tracking
    Kalina Peneva
    Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
    Angew Chem Int Ed Engl 47:3372-5. 2008
  66. doi request reprint Probing dimerization and intraprotein fluorescence resonance energy transfer in a far-red fluorescent protein from the sea anemone Heteractis crispa
    Guillaume A Lessard
    Los Alamos National Laboratory, Mail Stop J567, Los Alamos, New Mexico 87545, USA
    J Biomed Opt 13:031212. 2008
    ..This study will help in future quantitative microscopy investigations that use HcRed as a fluorescent marker...
  67. pmc Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants
    Ryoko Ando
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, The Institute of Physical and Chemical Research, Hirosawa, Wako City, Saitama 351 0198, Japan
    Biophys J 92:L97-9. 2007
    ..Simultaneous 488- and 405-nm irradiation, however, results in a rapid oscillation between the two states, thereby keeping the emissive state population large enough to produce sufficiently bright fluorescence signals...