Rik Gijsbers

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. pmc Role of the PWWP domain of lens epithelium-derived growth factor (LEDGF)/p75 cofactor in lentiviral integration targeting
    Rik Gijsbers
    Division of Molecular Medicine, KU Leuven, 3000 Leuven, Belgium
    J Biol Chem 286:41812-25. 2011
  2. ncbi request reprint A p-[18F]fluoroethoxyphenyl bicyclic nucleoside analogue as a potential positron emission tomography imaging agent for varicella-zoster virus thymidine kinase gene expression
    Satish K Chitneni
    Molecular Small Animal Imaging Center, Laboratory for Radiopharmacy Faculty of Pharmaceutical Sciences, Department of Nuclear Medicine, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium
    J Med Chem 50:6627-37. 2007
  3. pmc The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering
    Jelle Hendrix
    Laboratory for Biomolecular Dynamics, University of Leuven, Leuven, Flanders, B 3000, Belgium
    Nucleic Acids Res 39:1310-25. 2011
  4. doi request reprint Synthesis and biological evaluation of (11)C-labeled beta-galactosyl triazoles as potential PET tracers for in vivo LacZ reporter gene imaging
    Sofie Celen
    Laboratory for Radiopharmacy, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49 bus 821, BE 3000 Leuven, Belgium
    Bioorg Med Chem 17:5117-25. 2009
  5. pmc Virus evolution reveals an exclusive role for LEDGF/p75 in chromosomal tethering of HIV
    Anneleen Hombrouck
    The Laboratory for Molecular Virology and Gene Therapy, KULeuven and IRC KULAK, Leuven, Flanders, Belgium
    PLoS Pathog 3:e47. 2007
  6. pmc Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model
    Sofie Vets
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    Mol Ther 20:908-17. 2012
  7. ncbi request reprint Synthesis and biological evaluation of an 123I-labeled bicyclic nucleoside analogue (BCNA) as potential SPECT tracer for VZV-tk reporter gene imaging
    Satish K Chitneni
    Laboratory for Radiopharmacy, Katholieke Universiteit Leuven, Leuven, Belgium
    Bioorg Med Chem Lett 17:3458-62. 2007
  8. ncbi request reprint Differential interaction of HIV-1 integrase and JPO2 with the C terminus of LEDGF/p75
    Koen Bartholomeeusen
    Molecular Medicine, KULeuven and IRC KULAK, Leuven, Belgium
    J Mol Biol 372:407-21. 2007
  9. pmc LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin
    Rik Gijsbers
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    Mol Ther 18:552-60. 2010
  10. doi request reprint Characterization of rare lens epithelium-derived growth factor/p75 genetic variants identified in HIV-1 long-term nonprogressors
    Rik Schrijvers
    Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium
    AIDS 27:539-43. 2013

Collaborators

Detail Information

Publications35

  1. pmc Role of the PWWP domain of lens epithelium-derived growth factor (LEDGF)/p75 cofactor in lentiviral integration targeting
    Rik Gijsbers
    Division of Molecular Medicine, KU Leuven, 3000 Leuven, Belgium
    J Biol Chem 286:41812-25. 2011
    ....
  2. ncbi request reprint A p-[18F]fluoroethoxyphenyl bicyclic nucleoside analogue as a potential positron emission tomography imaging agent for varicella-zoster virus thymidine kinase gene expression
    Satish K Chitneni
    Molecular Small Animal Imaging Center, Laboratory for Radiopharmacy Faculty of Pharmaceutical Sciences, Department of Nuclear Medicine, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium
    J Med Chem 50:6627-37. 2007
    ..LC-MS analysis confirmed the formation of monophosphate derivative of 5 upon catalysis by VZV TK. In vivo studies of this new reporter gene/probe system are in progress...
  3. pmc The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering
    Jelle Hendrix
    Laboratory for Biomolecular Dynamics, University of Leuven, Leuven, Flanders, B 3000, Belgium
    Nucleic Acids Res 39:1310-25. 2011
    ..The PWWP is crucial for locking the complex on chromatin. We propose a scan-and-lock model for LEDGF/p75, unifying paradoxical notions of transcriptional co-activation and lentiviral integration targeting...
  4. doi request reprint Synthesis and biological evaluation of (11)C-labeled beta-galactosyl triazoles as potential PET tracers for in vivo LacZ reporter gene imaging
    Sofie Celen
    Laboratory for Radiopharmacy, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49 bus 821, BE 3000 Leuven, Belgium
    Bioorg Med Chem 17:5117-25. 2009
    ..In vitro evaluation in HEK-293T cells showed an increased cell uptake for the more lipophilic [(11)C]-13, however, there was no statistically higher uptake in LacZ expressing cells compared to control cells...
  5. pmc Virus evolution reveals an exclusive role for LEDGF/p75 in chromosomal tethering of HIV
    Anneleen Hombrouck
    The Laboratory for Molecular Virology and Gene Therapy, KULeuven and IRC KULAK, Leuven, Flanders, Belgium
    PLoS Pathog 3:e47. 2007
    ..Moreover, the role of LEDGF/p75 in targeting integration will stimulate research on strategies to direct gene therapy vectors into safe landing sites...
  6. pmc Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model
    Sofie Vets
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    Mol Ther 20:908-17. 2012
    ..LEDGF(325-530) overexpression potently inhibits HIV replication in vivo and protects against HIV mediated cell killing of primary CD(4)(+) T-cells...
  7. ncbi request reprint Synthesis and biological evaluation of an 123I-labeled bicyclic nucleoside analogue (BCNA) as potential SPECT tracer for VZV-tk reporter gene imaging
    Satish K Chitneni
    Laboratory for Radiopharmacy, Katholieke Universiteit Leuven, Leuven, Belgium
    Bioorg Med Chem Lett 17:3458-62. 2007
    ..2 microM). Biodistribution of [(123)I]-4 was examined in normal mice. Evaluation of [(123)I]-4 in HEK-293T cells showed 1.74-fold higher accumulation in VZV-TK-expressing cells compared to control cells...
  8. ncbi request reprint Differential interaction of HIV-1 integrase and JPO2 with the C terminus of LEDGF/p75
    Koen Bartholomeeusen
    Molecular Medicine, KULeuven and IRC KULAK, Leuven, Belgium
    J Mol Biol 372:407-21. 2007
    ..Furthermore, JPO2 over-expression activated transcription from the HIV-1 LTR...
  9. pmc LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin
    Rik Gijsbers
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    Mol Ther 18:552-60. 2010
    ..Thus, engineered LEDGF/p75 chimeras provide technology for controlling integration site selection by lentiviral vectors...
  10. doi request reprint Characterization of rare lens epithelium-derived growth factor/p75 genetic variants identified in HIV-1 long-term nonprogressors
    Rik Schrijvers
    Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium
    AIDS 27:539-43. 2013
    ..Here, we study two LEDGF/p75 exonic variants I436S and T473I, identified in HIV-1 long-term nonprogressors, together with Q472L...
  11. pmc LEDGF/p75-independent HIV-1 replication demonstrates a role for HRP-2 and remains sensitive to inhibition by LEDGINs
    Rik Schrijvers
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Flanders, Belgium
    PLoS Pathog 8:e1002558. 2012
    ..These results further support the potential of LEDGINs as allosteric integrase inhibitors...
  12. doi request reprint Highly efficient multicistronic lentiviral vectors with peptide 2A sequences
    Abdelilah Ibrahimi
    Molecular Medicine, Katholieke Universiteit Leuven, B 3000 Leuven, Flanders, Belgium
    Hum Gene Ther 20:845-60. 2009
    ..Last, using various peptide 2A sequences, we engineered the triple reporter LV-3R (i.e., eGFP, fLuc, HSV1-sr39tk), enabling efficient multimodality readouts in vivo...
  13. pmc HRP-2 determines HIV-1 integration site selection in LEDGF/p75 depleted cells
    Rik Schrijvers
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Flanders, Belgium
    Retrovirology 9:84. 2012
    ..We previously identified Hepatoma-derived growth factor related protein 2 (HRP-2) as a factor mediating LEDGF/p75-independent HIV-1 replication. However, the role of HRP-2 in HIV-1 integration site selection was not addressed...
  14. doi request reprint Transportin-SR2 imports HIV into the nucleus
    Frauke Christ
    Laboratory for Molecular Virology and Gene Therapy, KU Leuven and IRC, Kapucijnenvoer 33, B 3000 Leuven, Flanders, Belgium
    Curr Biol 18:1192-202. 2008
    ..This requires import of the preintegration complex (PIC) through the nuclear pore. Although many cellular and viral determinants have been proposed, the mechanism leading to nuclear import is not yet understood...
  15. doi request reprint A PET brain reporter gene system based on type 2 cannabinoid receptors
    Caroline Vandeputte
    Division of Nuclear Medicine, University Hospital and K U Leuven, Leuven, Belgium
    J Nucl Med 52:1102-9. 2011
    ..Here we report the first, to our knowledge, PET reporter system for imaging gene expression in the intact brain...
  16. doi request reprint Synthesis and evaluation of 18F- and 11C-labeled phenyl-galactopyranosides as potential probes for in vivo visualization of LacZ gene expression using positron emission tomography
    Sofie Celen
    Laboratory for Radiopharmacy, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Leuven, Belgium
    Bioconjug Chem 19:441-9. 2008
    ..Further structural modifications to improve the diffusion over the tumor cell membrane and to increase retention in beta-gal expressing cells may lead to more favorable in vivo imaging probes...
  17. doi request reprint Preliminary validation of varicella zoster virus thymidine kinase as a novel reporter gene for PET
    Christophe M Deroose
    Division of Nuclear Medicine, University Hospital Leuven, Leuven, Belgium
    Nucl Med Biol 39:1266-74. 2012
    ..We investigated varicella zoster virus thymidine kinase (VZV-tk) as new PRG with radiolabeled bicyclic nucleoside analogues (BCNAs) as PET tracers...
  18. doi request reprint Noninvasive and quantitative monitoring of adult neuronal stem cell migration in mouse brain using bioluminescence imaging
    Veerle Reumers
    Neurobiology and Gene Therapy, Katholieke Universiteit Leuven, Leuven, Flanders, Belgium
    Stem Cells 26:2382-90. 2008
    ..Long-term expression of brain-derived neurotrophic factor in the subventricular zone attenuated neurogenesis, as detected by histology and BLI. In vivo monitoring of the impact of drugs or genes on adult neurogenesis is now within reach...
  19. pmc LEDGINs inhibit late stage HIV-1 replication by modulating integrase multimerization in the virions
    Belete Ayele Desimmie
    Department of Pharmaceutical and Pharmacological Sciences, Laboratory for Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium
    Retrovirology 10:57. 2013
    ..They block HIV-1 integration by abrogating the interaction between LEDGF/p75 and IN as well as by allosterically inhibiting the catalytic activity of IN...
  20. pmc Interplay between HIV entry and transportin-SR2 dependency
    Wannes Thys
    Laboratory of Molecular Virology and Gene Therapy, Katholieke Universiteit Leuven, Kapucijnenvoer 33, VCTB 5, B 3000 Leuven, Flanders, Belgium
    Retrovirology 8:7. 2011
    ....
  21. pmc Efficient gene transfer into the mouse lung by fetal intratracheal injection of rAAV2/6.2
    Marianne Carlon
    Department of Molecular and Cellular Medicine, Katholieke Universiteit Leuven, Flanders, Belgium
    Mol Ther 18:2130-8. 2010
    ....
  22. pmc Comparison of lentiviral vector titration methods
    Martine Geraerts
    Laboratory for Molecular Virology and Gene Therapy, K U Leuven and IRC KULAK, Flanders, Belgium
    BMC Biotechnol 6:34. 2006
    ..Methods are required to analyze the quality of lentiviral vector production, the efficiency of gene transfer and the extent of therapeutic gene expression...
  23. pmc Transient and stable knockdown of the integrase cofactor LEDGF/p75 reveals its role in the replication cycle of human immunodeficiency virus
    Linos Vandekerckhove
    Molecular Medicine, K U Leuven and IRC KULAK, Kapucijnenvoer 33, B 3000 Leuven, Flanders, Belgium
    J Virol 80:1886-96. 2006
    ..Altogether, our data validate LEDGF/p75 as an important cellular cofactor for HIV integration and as a potential target for antiviral drug development...
  24. ncbi request reprint Parkin protects against neurotoxicity in the 6-hydroxydopamine rat model for Parkinson's disease
    Linda Vercammen
    Laboratory for Neurobiology and Gene Therapy, Molecular Medicine, K U Leuven, Kapucijnenvoer 33 VCTB 5, B 3000 Leuven, Flanders, Belgium
    Mol Ther 14:716-23. 2006
    ..These data support the therapeutic potential of parkin for the treatment of not only familial but also sporadic Parkinson's disease...
  25. doi request reprint The BET family of proteins targets moloney murine leukemia virus integration near transcription start sites
    Jan De Rijck
    Laboratory for Molecular Virology and Gene Therapy, KU Leuven, 3000 Leuven, Belgium
    Cell Rep 5:886-94. 2013
    ..Together, these data validate BET proteins as MLV integration targeting factors...
  26. doi request reprint PEAR1 attenuates megakaryopoiesis via control of the PI3K/PTEN pathway
    Alexandre Kauskot
    Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium
    Blood 121:5208-17. 2013
    ..In summary, this is the first demonstration that PEAR1 influences the PI3K/PTEN pathway, a critical determinant of Akt phosphorylation, itself controlling megakaryopoiesis and thrombopoiesis. ..
  27. pmc Lens epithelium-derived growth factor/p75 interacts with the transposase-derived DDE domain of PogZ
    Koen Bartholomeeusen
    Laboratory for Molecular Virology and Gene Therapy, Division of Molecular Medicine, Katholieke Universiteit Leuven, Kapucijnenvoer 33, Flanders, Belgium
    J Biol Chem 284:11467-77. 2009
    ..The finding that LEDGF/p75 is capable of interacting with a DDE domain protein that is not a lentiviral integrase points to a profound role of LEDGF/p75 in DDE domain protein function...
  28. pmc Phage display-directed discovery of LEDGF/p75 binding cyclic peptide inhibitors of HIV replication
    Belete A Desimmie
    Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, K U Leuven and IRC KULAK, Flanders, Belgium
    Mol Ther 20:2064-75. 2012
    ..Our work provides proof-of-concept for direct targeting of LEDGF/p75 as novel therapeutic strategy and the CPs thereby serve as scaffold for future development of new HIV therapeutics...
  29. doi request reprint Viral vectors expressing a single microRNA-based short-hairpin RNA result in potent gene silencing in vitro and in vivo
    Luísa Osório
    KU Leuven, Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, Leuven, Belgium
    J Biotechnol 169:71-81. 2014
    ....
  30. pmc Transient Expression of an LEDGF/p75 Chimera Retargets Lentivector Integration and Functionally Rescues in a Model for X-CGD
    Sofie Vets
    Department of Pharmaceutical and Pharmacological Sciences, Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Belgium
    Mol Ther Nucleic Acids 2:e77. 2013
    ..Molecular Therapy - Nucleic Acids (2013) 2, e77; doi:10.1038/mtna.2013.4; published online 5 March 2013...
  31. pmc Immunohistochemical detection of transgene expression in the brain using small epitope tags
    Evy Lobbestael
    Laboratory for Neurobiology and Gene Therapy, Division of Molecular Medicine, Department of Molecular and Cellular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    BMC Biotechnol 10:16. 2010
    ..In order to minimize impact on the bioactivity and biodistribution of the overexpressed protein, preference is given to small tags...
  32. doi request reprint Recipient leukocyte infusion enhances the local and systemic graft-versus-neuroblastoma effect of allogeneic bone marrow transplantation in mice
    Leen Willems
    Laboratory of Experimental Transplantation, KU Leuven, Herestraat 49, Box 811, 3000, Leuven, Belgium
    Cancer Immunol Immunother 62:1733-44. 2013
    ..Our data indicate that RLI may provide combined T-cell and NK-cell reactivity effectively targeting Neuro2A neuroblastoma...
  33. ncbi request reprint Synthesis and preliminary evaluation of 18F- or 11C-labeled bicyclic nucleoside analogues as potential probes for imaging varicella-zoster virus thymidine kinase gene expression using positron emission tomography
    Satish K Chitneni
    Laboratory for Radiopharmacy, Faculty of Pharmaceutical Sciences, K U Leuven, Leuven, Belgium
    J Med Chem 50:1041-9. 2007
    ..These data warrant further evaluation of these tracers as noninvasive imaging agents for VZV infection and VZV-tk reporter gene expression in vivo...
  34. pmc miR669a and miR669q prevent skeletal muscle differentiation in postnatal cardiac progenitors
    Stefania Crippa
    Translational Cardiomyology Laboratory, Interdepartmental Stem Cell Institute, Catholic University of Leuven, 3000 Leuven, Belgium
    J Cell Biol 193:1197-212. 2011
    ....
  35. doi request reprint Regulator of G-protein signaling 18 controls megakaryopoiesis and the cilia-mediated vertebrate mechanosensory system
    Sophie Louwette
    Center for Molecular and Vascular Biology, KU Leuven, Campus Gasthuisberg, O and N1, Herestraat 49, Box 911, 3000 Leuven, Belgium
    FASEB J 26:2125-36. 2012
    ..In summary, our study is the first to show that RGS18 regulates megakaryopoiesis but also reveals its unexpected role in ciliogenesis, at least in lower vertebrates, via interference with Wnt signaling...