K Gebruers

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Properties of TAXI-type endoxylanase inhibitors
    Kurt Gebruers
    KU Leuven, Laboratory of Food Chemistry, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Biochim Biophys Acta 1696:213-21. 2004
  2. doi request reprint Algerian pearl millet ( Pennisetum glaucum L.) contains XIP but not TAXI and TLXI type xylanase inhibitors
    Hind Mokrane
    Laboratoire des Produits Bioactifs et de la Valorisation de la Biomasse, Ecole Normale Superieure, Vieux Kouba, Alger, Algeria
    J Agric Food Chem 57:5542-8. 2009
  3. pmc Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificities
    K Gebruers
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Heverlee, Belgium
    Biochem J 353:239-44. 2001
  4. ncbi request reprint Purification of TAXI-like endoxylanase inhibitors from wheat (Triticum aestivum L.) whole meal reveals a family of iso-forms
    Kurt Gebruers
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Belgium
    J Enzyme Inhib Med Chem 17:61-8. 2002
  5. ncbi request reprint Crystallization and preliminary X-ray diffraction study of a wheat (Triticum aestivum L.) TAXI-type endoxylanase inhibitor
    Stefaan Sansen
    Laboratorium voor Analytische Chemie en Medicinale Fysiochemie, K U Leuven, Belgium
    Acta Crystallogr D Biol Crystallogr 59:744-6. 2003
  6. ncbi request reprint Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificity
    Gert Raedschelders
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Biochem Biophys Res Commun 335:512-22. 2005
  7. ncbi request reprint His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases
    Katleen Fierens
    Katholieke Universiteit Leuven, Laboratory of Food Chemistry, Leuven, Belgium
    FEBS J 272:5872-82. 2005
  8. ncbi request reprint Insight into the distribution of arabinoxylans, endoxylanases, and endoxylanase inhibitors in industrial wheat roller mill streams
    Emmie Dornez
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    J Agric Food Chem 54:8521-9. 2006
  9. pmc TLXI, a novel type of xylanase inhibitor from wheat (Triticum aestivum) belonging to the thaumatin family
    Ellen Fierens
    Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    Biochem J 403:583-91. 2007
  10. ncbi request reprint Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme
    Steven Van Campenhout
    Laboratory of Gene Technology, K U Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Biochem Biophys Res Commun 356:799-804. 2007

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Properties of TAXI-type endoxylanase inhibitors
    Kurt Gebruers
    KU Leuven, Laboratory of Food Chemistry, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Biochim Biophys Acta 1696:213-21. 2004
    ....
  2. doi request reprint Algerian pearl millet ( Pennisetum glaucum L.) contains XIP but not TAXI and TLXI type xylanase inhibitors
    Hind Mokrane
    Laboratoire des Produits Bioactifs et de la Valorisation de la Biomasse, Ecole Normale Superieure, Vieux Kouba, Alger, Algeria
    J Agric Food Chem 57:5542-8. 2009
    ..longibrachiatum GH family 11 xylanase. On the basis of immunoblot neither TAXI nor TLXI type xylanase inhibitors were detected in pearl millet grains...
  3. pmc Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificities
    K Gebruers
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Heverlee, Belgium
    Biochem J 353:239-44. 2001
    ..The N-terminal amino acid sequences of both inhibitors show a high degree of identity, which might indicate that there is an evolutionary relationship between them...
  4. ncbi request reprint Purification of TAXI-like endoxylanase inhibitors from wheat (Triticum aestivum L.) whole meal reveals a family of iso-forms
    Kurt Gebruers
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Belgium
    J Enzyme Inhib Med Chem 17:61-8. 2002
    ..The first type of endoxylanase inhibitor is active against both endoxylanases and the second type only has significant activity against the B. subtilis endoxylanase...
  5. ncbi request reprint Crystallization and preliminary X-ray diffraction study of a wheat (Triticum aestivum L.) TAXI-type endoxylanase inhibitor
    Stefaan Sansen
    Laboratorium voor Analytische Chemie en Medicinale Fysiochemie, K U Leuven, Belgium
    Acta Crystallogr D Biol Crystallogr 59:744-6. 2003
    ..25 and 1.75 A resolution, respectively. The heavy-atom derivative of this crystal form was obtained by the soaking method and allowed determination of the initial phases...
  6. ncbi request reprint Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificity
    Gert Raedschelders
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Biochem Biophys Res Commun 335:512-22. 2005
    ..These results show that TAXI-II inhibition specificity bears on the identity of two key residues at positions 294 and 376, which are involved in the interaction at the -2 glycon subsite and the active site of GHF 11, respectively...
  7. ncbi request reprint His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases
    Katleen Fierens
    Katholieke Universiteit Leuven, Laboratory of Food Chemistry, Leuven, Belgium
    FEBS J 272:5872-82. 2005
    ..In addition, the results show that His374 plays a critical role in the stabilization of the endoxylanase-TAXI-I complex rather than in the docking of inhibitor onto enzyme...
  8. ncbi request reprint Insight into the distribution of arabinoxylans, endoxylanases, and endoxylanase inhibitors in industrial wheat roller mill streams
    Emmie Dornez
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    J Agric Food Chem 54:8521-9. 2006
    ..3-fold and were not correlated with any of the parameters measured in this study. The results can be valuable in blending and optimizing wheat flour fractions to obtain flours with specific technological and nutritional benefits...
  9. pmc TLXI, a novel type of xylanase inhibitor from wheat (Triticum aestivum) belonging to the thaumatin family
    Ellen Fierens
    Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    Biochem J 403:583-91. 2007
    ..60-nM. Except for zeamatin, an alpha-amylase/trypsin inhibitor from maize (Zea mays), no other enzyme inhibitor is currently known among the TLPs. TLXI thus represents a novel type of inhibitor within this group of proteins...
  10. ncbi request reprint Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme
    Steven Van Campenhout
    Laboratory of Gene Technology, K U Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Biochem Biophys Res Commun 356:799-804. 2007
    ..The implications of these findings in relation to the putative role of the N-terminal propeptide as a carbohydrate binding module and the possible consequences for the way X-I fulfils its role in the germination process, are discussed...
  11. ncbi request reprint Indirect enzyme-antibody sandwich enzyme-linked immunosorbent assay for quantification of TAXI and XIP type xylanase inhibitors in wheat and other cereals
    Johnny Beaugrand
    Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 30, B 3001 Leuven, Belgium
    J Agric Food Chem 55:7682-8. 2007
    ..The TAXI ELISA for wheat was successfully adapted for barley ( Hordeum vulgare) and could also be used for other cereals...
  12. doi request reprint Variability of polymorphic families of three types of xylanase inhibitors in the wheat grain proteome
    Evi Croes
    Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Leuven, Belgium
    Proteomics 8:1692-705. 2008
    ..Besides genetic diversity, PTMs, such as glycosylation, account for the additional complexity of the 2-DE patterns...
  13. ncbi request reprint A family 11 xylanase from Penicillium funiculosum is strongly inhibited by three wheat xylanase inhibitors
    Caroline S M Furniss
    Nutrition and Consumer Sciences Division, Institute of Food Research, Norwich Research Park, Colney, Norwich, UK
    Biochim Biophys Acta 1598:24-9. 2002
    ..This is the first report describing a xylanase that is inhibited by all three wheat xylanase inhibitor proteins described to date...
  14. ncbi request reprint Functional importance of Asp37 from a family 11 xylanase in the binding to two proteinaceous xylanase inhibitors from wheat
    Tariq A Tahir
    Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
    FEMS Microbiol Lett 239:9-15. 2004
    ..The results are consistent with a competitive inhibition mode and underline the strategic importance of Asp37 in the inhibition mechanism...
  15. ncbi request reprint Molecular identification of wheat endoxylanase inhibitor TAXI-I1, member of a new class of plant proteins
    Katleen Fierens
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    FEBS Lett 540:259-63. 2003
    ..Our data indicate that TAXI-I belongs to a newly identified class of plant proteins for which a molecular function as glycoside hydrolase inhibitor can now be suggested...
  16. ncbi request reprint TAXI type endoxylanase inhibitors in different cereals
    Hans Goesaert
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Belgium
    J Agric Food Chem 51:3770-5. 2003
    ..For each cereal, high-resolution cation exchange chromatography revealed the presence of multiple isoinhibitors, each of which occurs in two molecular forms. However, in durum wheat and barley, a single isoform is predominantly present...
  17. ncbi request reprint Occurrence of proteinaceous endoxylanase inhibitors in cereals
    Hans Goesaert
    KU Leuven, Laboratory of Food Chemistry, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Biochim Biophys Acta 1696:193-202. 2004
    ..Lower inhibitor levels are present in durum wheat and barley, while maize contains solely XIP-type inhibitors. No inhibitors have been isolated from rice, oats and buckwheat...
  18. ncbi request reprint Potential physiological role of plant glycosidase inhibitors
    Daniela Bellincampi
    Dipartimento di Biologia Vegetale, Universita di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
    Biochim Biophys Acta 1696:265-74. 2004
    ..Results recently obtained with plant transformation technology indicate that this class of inhibitors has potential biotechnological applications...
  19. ncbi request reprint Crystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilis
    Stefaan Sansen
    Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, K U Leuven, E Van Evenstraat 4, B 3000 Leuven, Belgium
    Acta Crystallogr D Biol Crystallogr 60:555-7. 2004
    ..subtilis xylanase crystallizes in the monoclinic space group C2, with a = 107.89, b = 95.33, c = 66.31 A, beta = 122.24 degrees. Complete data sets were collected for both crystal types using synchrotron radiation...
  20. ncbi request reprint Structural basis for inhibition of Aspergillus niger xylanase by triticum aestivum xylanase inhibitor-I
    Stefaan Sansen
    Laboratory of Analytical Chemistry and Medicinal Physicochemistry, Katholieke Universiteit Leuven, E Van Evenstraat 4, B 3000 Leuven, Belgium
    J Biol Chem 279:36022-8. 2004
    ..8 A, illustrates the ability of tight binding and inhibition with subnanomolar affinity and indicates the importance of the C-terminal end for the differences in xylanase specificity among different TAXI-type inhibitors...
  21. ncbi request reprint High-level expression, purification, and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastoris
    Katleen Fierens
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Protein Expr Purif 37:39-46. 2004
    ..The present results show that xylanase inhibition activity of TAXI-I is only affected to a limited degree by its glycosylation or proteolytic processing...
  22. ncbi request reprint Purification and characterization of a XIP-type endoxylanase inhibitor from rice (Oryza sativa)
    Hans Goesaert
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    J Enzyme Inhib Med Chem 20:95-101. 2005
    ..were not sensitive to inhibition. The above mentioned A. aculeatus endoxylanase was not inhibited either, although gel permeation chromatography revealed that it complexed rice XIP in a 1:1 molar stoichiometric ratio...
  23. ncbi request reprint Wheat-kernel-associated endoxylanases consist of a majority of microbial and a minority of wheat endogenous endoxylanases
    Emmie Dornez
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    J Agric Food Chem 54:4028-34. 2006
    ..Results showed that microbial endoxylanases can account for over 90% of the total wheat-associated endoxylanase activity and that the latter can be at least 5 times higher than the apparent endoxylanase activity...
  24. ncbi request reprint Impact of wheat flour-associated endoxylanases on arabinoxylan in dough after mixing and resting
    Emmie Dornez
    Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    J Agric Food Chem 55:7149-55. 2007
    ..The results indicate that wheat flour-associated endoxylanases can alter part of the AX in dough, thereby changing their functionality in bread making and potentially affecting dough and end product properties...
  25. ncbi request reprint Xylanase inhibitors bind to nonstarch polysaccharides
    Ellen Fierens
    Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Box 2463, 3001 Leuven, Belgium
    J Agric Food Chem 56:564-70. 2008
    ....
  26. doi request reprint Contribution of wheat endogenous and wheat kernel associated microbial endoxylanases to changes in the arabinoxylan population during breadmaking
    Emmie Dornez
    Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    J Agric Food Chem 56:2246-53. 2008
    ....