Research Topics
Genomes and GenesSpecies | K GebruersSummaryAffiliation: Katholieke Universiteit Leuven Country: Belgium Publications
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Publications
Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificitiesK Gebruers
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B 3001 Heverlee, Belgium
Biochem J 353:239-44. 2001..The N-terminal amino acid sequences of both inhibitors show a high degree of identity, which might indicate that there is an evolutionary relationship between them...
Purification of TAXI-like endoxylanase inhibitors from wheat (Triticum aestivum L.) whole meal reveals a family of iso-formsKurt Gebruers
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Belgium
J Enzyme Inhib Med Chem 17:61-8. 2002..The first type of endoxylanase inhibitor is active against both endoxylanases and the second type only has significant activity against the B. subtilis endoxylanase...- Properties of TAXI-type endoxylanase inhibitorsKurt Gebruers
KU Leuven, Laboratory of Food Chemistry, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
Biochim Biophys Acta 1696:213-21. 2004.... - Purification and characterization of a XIP-type endoxylanase inhibitor from rice (Oryza sativa)Hans Goesaert
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
J Enzyme Inhib Med Chem 20:95-101. 2005..were not sensitive to inhibition. The above mentioned A. aculeatus endoxylanase was not inhibited either, although gel permeation chromatography revealed that it complexed rice XIP in a 1:1 molar stoichiometric ratio... - Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificityGert Raedschelders
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium
Biochem Biophys Res Commun 335:512-22. 2005..These results show that TAXI-II inhibition specificity bears on the identity of two key residues at positions 294 and 376, which are involved in the interaction at the -2 glycon subsite and the active site of GHF 11, respectively... - His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanasesKatleen Fierens
Katholieke Universiteit Leuven, Laboratory of Food Chemistry, Leuven, Belgium
FEBS J 272:5872-82. 2005..In addition, the results show that His374 plays a critical role in the stabilization of the endoxylanase-TAXI-I complex rather than in the docking of inhibitor onto enzyme... - Insight into the distribution of arabinoxylans, endoxylanases, and endoxylanase inhibitors in industrial wheat roller mill streamsEmmie Dornez
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium
J Agric Food Chem 54:8521-9. 2006..3-fold and were not correlated with any of the parameters measured in this study. The results can be valuable in blending and optimizing wheat flour fractions to obtain flours with specific technological and nutritional benefits... - TLXI, a novel type of xylanase inhibitor from wheat (Triticum aestivum) belonging to the thaumatin familyEllen Fierens
Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
Biochem J 403:583-91. 2007..60-nM. Except for zeamatin, an alpha-amylase/trypsin inhibitor from maize (Zea mays), no other enzyme inhibitor is currently known among the TLPs. TLXI thus represents a novel type of inhibitor within this group of proteins... - Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzymeSteven Van Campenhout
Laboratory of Gene Technology, K U Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
Biochem Biophys Res Commun 356:799-804. 2007..The implications of these findings in relation to the putative role of the N-terminal propeptide as a carbohydrate binding module and the possible consequences for the way X-I fulfils its role in the germination process, are discussed... - Indirect enzyme-antibody sandwich enzyme-linked immunosorbent assay for quantification of TAXI and XIP type xylanase inhibitors in wheat and other cerealsJohnny Beaugrand
Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 30, B 3001 Leuven, Belgium
J Agric Food Chem 55:7682-8. 2007..The TAXI ELISA for wheat was successfully adapted for barley ( Hordeum vulgare) and could also be used for other cereals... - Functional importance of Asp37 from a family 11 xylanase in the binding to two proteinaceous xylanase inhibitors from wheatTariq A Tahir
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
FEMS Microbiol Lett 239:9-15. 2004..The results are consistent with a competitive inhibition mode and underline the strategic importance of Asp37 in the inhibition mechanism... - High-level expression, purification, and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastorisKatleen Fierens
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
Protein Expr Purif 37:39-46. 2004..The present results show that xylanase inhibition activity of TAXI-I is only affected to a limited degree by its glycosylation or proteolytic processing... - Structural basis for inhibition of Aspergillus niger xylanase by triticum aestivum xylanase inhibitor-IStefaan Sansen
Laboratory of Analytical Chemistry and Medicinal Physicochemistry, Katholieke Universiteit Leuven, E. Van Evenstraat 4, B-3000 Leuven, Belgium
J Biol Chem 279:36022-8. 2004..8 A, illustrates the ability of tight binding and inhibition with subnanomolar affinity and indicates the importance of the C-terminal end for the differences in xylanase specificity among different TAXI-type inhibitors... - A family 11 xylanase from Penicillium funiculosum is strongly inhibited by three wheat xylanase inhibitorsCaroline S M Furniss
Nutrition and Consumer Sciences Division, Institute of Food Research, Norwich Research Park, Colney, Norwich, UK
Biochim Biophys Acta 1598:24-9. 2002..This is the first report describing a xylanase that is inhibited by all three wheat xylanase inhibitor proteins described to date... - Crystallization and preliminary X-ray diffraction study of a wheat (Triticum aestivum L.) TAXI-type endoxylanase inhibitorStefaan Sansen
Laboratorium voor Analytische Chemie en Medicinale Fysiochemie, K.U. Leuven, Belgium
Acta Crystallogr D Biol Crystallogr 59:744-6. 2003..25 and 1.75 A resolution, respectively. The heavy-atom derivative of this crystal form was obtained by the soaking method and allowed determination of the initial phases... - Molecular identification of wheat endoxylanase inhibitor TAXI-I1, member of a new class of plant proteinsKatleen Fierens
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
FEBS Lett 540:259-63. 2003..Our data indicate that TAXI-I belongs to a newly identified class of plant proteins for which a molecular function as glycoside hydrolase inhibitor can now be suggested... - TAXI type endoxylanase inhibitors in different cerealsHans Goesaert
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Belgium
J Agric Food Chem 51:3770-5. 2003..For each cereal, high-resolution cation exchange chromatography revealed the presence of multiple isoinhibitors, each of which occurs in two molecular forms. However, in durum wheat and barley, a single isoform is predominantly present... - Occurrence of proteinaceous endoxylanase inhibitors in cerealsHans Goesaert
KU Leuven, Laboratory of Food Chemistry, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
Biochim Biophys Acta 1696:193-202. 2004..Lower inhibitor levels are present in durum wheat and barley, while maize contains solely XIP-type inhibitors. No inhibitors have been isolated from rice, oats and buckwheat... - Potential physiological role of plant glycosidase inhibitorsDaniela Bellincampi
Dipartimento di Biologia Vegetale, Universita di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
Biochim Biophys Acta 1696:265-74. 2004..Results recently obtained with plant transformation technology indicate that this class of inhibitors has potential biotechnological applications... - Crystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilisStefaan Sansen
Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, K.U. Leuven, E. Van Evenstraat 4, B-3000 Leuven, Belgium
Acta Crystallogr D Biol Crystallogr 60:555-7. 2004..subtilis xylanase crystallizes in the monoclinic space group C2, with a = 107.89, b = 95.33, c = 66.31 A, beta = 122.24 degrees. Complete data sets were collected for both crystal types using synchrotron radiation... 
Variability of polymorphic families of three types of xylanase inhibitors in the wheat grain proteomeEvi Croes
Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Leuven, Belgium
Proteomics 8:1692-705. 2008..Besides genetic diversity, PTMs, such as glycosylation, account for the additional complexity of the 2-DE patterns...- Wheat-kernel-associated endoxylanases consist of a majority of microbial and a minority of wheat endogenous endoxylanasesEmmie Dornez
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium
J Agric Food Chem 54:4028-34. 2006..Results showed that microbial endoxylanases can account for over 90% of the total wheat-associated endoxylanase activity and that the latter can be at least 5 times higher than the apparent endoxylanase activity... - Impact of wheat flour-associated endoxylanases on arabinoxylan in dough after mixing and restingEmmie Dornez
Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
J Agric Food Chem 55:7149-55. 2007..The results indicate that wheat flour-associated endoxylanases can alter part of the AX in dough, thereby changing their functionality in bread making and potentially affecting dough and end product properties... - Xylanase inhibitors bind to nonstarch polysaccharidesEllen Fierens
Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Box 2463, 3001 Leuven, Belgium
J Agric Food Chem 56:564-70. 2008.... - Contribution of wheat endogenous and wheat kernel associated microbial endoxylanases to changes in the arabinoxylan population during breadmakingEmmie Dornez
Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
J Agric Food Chem 56:2246-53. 2008....