Katleen Fierens

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi Molecular identification of wheat endoxylanase inhibitor TAXI-I1, member of a new class of plant proteins
    Katleen Fierens
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    FEBS Lett 540:259-63. 2003
  2. ncbi High-level expression, purification, and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastoris
    Katleen Fierens
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Protein Expr Purif 37:39-46. 2004
  3. ncbi His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases
    Katleen Fierens
    Katholieke Universiteit Leuven, Laboratory of Food Chemistry, Leuven, Belgium
    FEBS J 272:5872-82. 2005
  4. ncbi Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificity
    Gert Raedschelders
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium
    Biochem Biophys Res Commun 335:512-22. 2005
  5. ncbi Properties of TAXI-type endoxylanase inhibitors
    Kurt Gebruers
    KU Leuven, Laboratory of Food Chemistry, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Biochim Biophys Acta 1696:213-21. 2004
  6. ncbi Targeted molecular engineering of a family 11 endoxylanase to decrease its sensitivity towards Triticum aestivum endoxylanase inhibitor types
    Tine M Bourgois
    Katholieke Universiteit Leuven, Laboratory of Gene Technology, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    J Biotechnol 130:95-105. 2007

Collaborators

Detail Information

Publications6

  1. ncbi Molecular identification of wheat endoxylanase inhibitor TAXI-I1, member of a new class of plant proteins
    Katleen Fierens
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Leuven, Belgium
    FEBS Lett 540:259-63. 2003
    ..Our data indicate that TAXI-I belongs to a newly identified class of plant proteins for which a molecular function as glycoside hydrolase inhibitor can now be suggested...
  2. ncbi High-level expression, purification, and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastoris
    Katleen Fierens
    Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Protein Expr Purif 37:39-46. 2004
    ..The present results show that xylanase inhibition activity of TAXI-I is only affected to a limited degree by its glycosylation or proteolytic processing...
  3. ncbi His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases
    Katleen Fierens
    Katholieke Universiteit Leuven, Laboratory of Food Chemistry, Leuven, Belgium
    FEBS J 272:5872-82. 2005
    ..In addition, the results show that His374 plays a critical role in the stabilization of the endoxylanase-TAXI-I complex rather than in the docking of inhibitor onto enzyme...
  4. ncbi Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificity
    Gert Raedschelders
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium
    Biochem Biophys Res Commun 335:512-22. 2005
    ..These results show that TAXI-II inhibition specificity bears on the identity of two key residues at positions 294 and 376, which are involved in the interaction at the -2 glycon subsite and the active site of GHF 11, respectively...
  5. ncbi Properties of TAXI-type endoxylanase inhibitors
    Kurt Gebruers
    KU Leuven, Laboratory of Food Chemistry, Kasteelpark Arenberg 20, B 3001 Louvain, Belgium
    Biochim Biophys Acta 1696:213-21. 2004
    ....
  6. ncbi Targeted molecular engineering of a family 11 endoxylanase to decrease its sensitivity towards Triticum aestivum endoxylanase inhibitor types
    Tine M Bourgois
    Katholieke Universiteit Leuven, Laboratory of Gene Technology, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    J Biotechnol 130:95-105. 2007
    ..Variants from the second strategy showed altered inhibitor sensitivities concomitant with changes in enzyme activities and allowed to gain insight in the binding-mode of both TAXI-I and TAXI-II with BSXY...