Frank Claessens

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Selective DNA binding by the androgen receptor as a mechanism for hormone-specific gene regulation
    F Claessens
    Laboratory for Biochemistry, Gasthuisberg, Catholic University of Leuven, Herestraat 49, B 3000, Leuven, Belgium
    J Steroid Biochem Mol Biol 76:23-30. 2001
  2. ncbi request reprint DNA recognition by nuclear receptors
    Frank Claessens
    Department of Molecular Cell Biology, Faculty of Medicine, Campus GHB O N, Herestraat 49, 3000 Leuven, Belgium
    Essays Biochem 40:59-72. 2004
  3. pmc Loss of androgen receptor binding to selective androgen response elements causes a reproductive phenotype in a knockin mouse model
    Kris Schauwaers
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, Catholic University of Leuven, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 104:4961-6. 2007
  4. doi request reprint A 629RKLKK633 motif in the hinge region controls the androgen receptor at multiple levels
    Tamzin M Tanner
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, Catholic University of Leuven, Campus Gasthuisberg O and N1, Herestraat 49, Box 901, 3000, Leuven, Belgium
    Cell Mol Life Sci 67:1919-27. 2010
  5. ncbi request reprint Identification of an androgen response element in intron 8 of the sterol regulatory element-binding protein cleavage-activating protein gene allowing direct regulation by the androgen receptor
    Hannelore Heemers
    Laboratory for Experimental Medicine and Endocrinology, Faculty of Medicine, Onderwijs en Navorsing, Gasthuisberg, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 279:30880-7. 2004
  6. doi request reprint The rules of DNA recognition by the androgen receptor
    Sarah Denayer
    Katholieke Universiteit Leuven, Campus Gasthuisberg, O N1, Herestraat 49, 3000 Leuven, Belgium
    Mol Endocrinol 24:898-913. 2010
  7. doi request reprint A role for selective androgen response elements in the development of the epididymis and the androgen control of the 5α reductase II gene
    Stefanie Kerkhofs
    Department of Cellular and Molecular Medicine, Katholieke Univesiteit Leuven, Campus Gasthuisberg O and N1 P O Box 901, Herestraat 49, 3000 Leuven, Belgium
    FASEB J 26:4360-72. 2012
  8. ncbi request reprint Implications of a polyglutamine tract in the function of the human androgen receptor
    Leen Callewaert
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg O N, University of Leuven, O N, Herestraat 49, B 3000, Louvain, Belgium
    Biochem Biophys Res Commun 306:46-52. 2003
  9. ncbi request reprint Characterization of the two coactivator-interacting surfaces of the androgen receptor and their relative role in transcriptional control
    Valerie Christiaens
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Herestraat 49, B 3000 Leuven, Belgium
    J Biol Chem 277:49230-7. 2002
  10. ncbi request reprint The hinge region regulates DNA binding, nuclear translocation, and transactivation of the androgen receptor
    Annemie Haelens
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Leuven, Belgium
    Cancer Res 67:4514-23. 2007

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Selective DNA binding by the androgen receptor as a mechanism for hormone-specific gene regulation
    F Claessens
    Laboratory for Biochemistry, Gasthuisberg, Catholic University of Leuven, Herestraat 49, B 3000, Leuven, Belgium
    J Steroid Biochem Mol Biol 76:23-30. 2001
    ....
  2. ncbi request reprint DNA recognition by nuclear receptors
    Frank Claessens
    Department of Molecular Cell Biology, Faculty of Medicine, Campus GHB O N, Herestraat 49, 3000 Leuven, Belgium
    Essays Biochem 40:59-72. 2004
    ..The DNA-binding domain therefore plays a central role, not only in the correct binding of the receptors to the target genes, but also in the control of other steps of the action mechanism of nuclear receptors...
  3. pmc Loss of androgen receptor binding to selective androgen response elements causes a reproductive phenotype in a knockin mouse model
    Kris Schauwaers
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, Catholic University of Leuven, Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 104:4961-6. 2007
    ..In this report, we present the first in vivo evidence of the existence of two functionally different types of AREs and demonstrate that AR-regulated gene expression can be targeted based on this distinction...
  4. doi request reprint A 629RKLKK633 motif in the hinge region controls the androgen receptor at multiple levels
    Tamzin M Tanner
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, Catholic University of Leuven, Campus Gasthuisberg O and N1, Herestraat 49, Box 901, 3000, Leuven, Belgium
    Cell Mol Life Sci 67:1919-27. 2010
    ..Taken together, the (629)RKLKK(633) motif is a multifunctional motif that integrates nuclear localization, receptor stability, DNA binding, transactivation potential and intranuclear mobility...
  5. ncbi request reprint Identification of an androgen response element in intron 8 of the sterol regulatory element-binding protein cleavage-activating protein gene allowing direct regulation by the androgen receptor
    Hannelore Heemers
    Laboratory for Experimental Medicine and Endocrinology, Faculty of Medicine, Onderwijs en Navorsing, Gasthuisberg, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 279:30880-7. 2004
    ..The identification of a steroid response element in intron 8 of the SCAP gene further supports the contention that SCAP is a direct target for steroid hormone action...
  6. doi request reprint The rules of DNA recognition by the androgen receptor
    Sarah Denayer
    Katholieke Universiteit Leuven, Campus Gasthuisberg, O N1, Herestraat 49, 3000 Leuven, Belgium
    Mol Endocrinol 24:898-913. 2010
    ..So far, all AR-binding genomic fragments that were analyzed contain AREs defined as receptor-dimer binding motifs with the ability to confer responsiveness to a reporter gene...
  7. doi request reprint A role for selective androgen response elements in the development of the epididymis and the androgen control of the 5α reductase II gene
    Stefanie Kerkhofs
    Department of Cellular and Molecular Medicine, Katholieke Univesiteit Leuven, Campus Gasthuisberg O and N1 P O Box 901, Herestraat 49, 3000 Leuven, Belgium
    FASEB J 26:4360-72. 2012
    ..We conclude that the SPARKI model can be considered a knockout model for AR functioning via selective AREs and that this has a dramatic effect on sperm maturation in the epididymis...
  8. ncbi request reprint Implications of a polyglutamine tract in the function of the human androgen receptor
    Leen Callewaert
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg O N, University of Leuven, O N, Herestraat 49, B 3000, Louvain, Belgium
    Biochem Biophys Res Commun 306:46-52. 2003
    ..This is reflected by an enhanced coactivation of the AR by SRC-1e...
  9. ncbi request reprint Characterization of the two coactivator-interacting surfaces of the androgen receptor and their relative role in transcriptional control
    Valerie Christiaens
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Herestraat 49, B 3000 Leuven, Belgium
    J Biol Chem 277:49230-7. 2002
    ..We propose, therefore, that the androgen receptor-coactivator complex has several alternative conformations, depending partially on the context of the enhancer...
  10. ncbi request reprint The hinge region regulates DNA binding, nuclear translocation, and transactivation of the androgen receptor
    Annemie Haelens
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Leuven, Belgium
    Cancer Res 67:4514-23. 2007
    ..In conclusion, the description of prostate cancer mutations has led to the discovery of a complex role of the hinge region in nuclear localization, DNA binding, coactivator recruitment, and N/C interaction of the AR...
  11. doi request reprint Expression of Tubb3, a beta-tubulin isotype, is regulated by androgens in mouse and rat Sertoli cells
    Karel De Gendt
    Laboratory of Experimental Medicine and Endocrinology, Catholic University of Leuven, Leuven, Belgium
    Biol Reprod 85:934-45. 2011
    ....
  12. doi request reprint Structural basis for nuclear hormone receptor DNA binding
    Christine Helsen
    Molecular Endocrinology Laboratory, Department Molecular Cell Biology, Campus GHB, ON1, Herestraat 49, 3000 Leuven, Belgium
    Mol Cell Endocrinol 348:411-7. 2012
    ..Moreover, allosteric signals passing through towards other receptor domains, explain why to some extent, the DNA elements can also be considered as controlling ligands...
  13. ncbi request reprint Dual function of an amino-terminal amphipatic helix in androgen receptor-mediated transactivation through specific and nonspecific response elements
    Leen Callewaert
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Herestraat 49, Belgium
    J Biol Chem 278:8212-8. 2003
    ....
  14. pmc DNA recognition by the androgen receptor: evidence for an alternative DNA-dependent dimerization, and an active role of sequences flanking the response element on transactivation
    Annemie Haelens
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem J 369:141-51. 2003
    ..In addition, the sequences flanking an androgen-response element can control the activity of the androgen receptor...
  15. doi request reprint Fracture risk and zoledronic acid therapy in men with osteoporosis
    Steven Boonen
    Katholieke Universiteit Leuven, Leuven, Belgium
    N Engl J Med 367:1714-23. 2012
    ..Fractures in men are a major health issue, and data on the antifracture efficacy of therapies for osteoporosis in men are limited. We studied the effect of zoledronic acid on fracture risk among men with osteoporosis...
  16. ncbi request reprint Functional interplay between two response elements with distinct binding characteristics dictates androgen specificity of the mouse sex-limited protein enhancer
    Guy Verrijdt
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Leuven B 3000, Belgium
    J Biol Chem 277:35191-201. 2002
    ....
  17. pmc Diverse roles of androgen receptor (AR) domains in AR-mediated signaling
    Frank Claessens
    Molecular Endocrinology Laboratory, Campus Gasthuisberg, University of Leuven, Leuven, Belgium
    Nucl Recept Signal 6:e008. 2008
    ....
  18. ncbi request reprint Interplay between two hormone-independent activation domains in the androgen receptor
    Leen Callewaert
    Molecular Endocrinology Laboratory, Faculty of Medicine, Campus Gasthuisberg, University of Leuven, Leuven, Belgium
    Cancer Res 66:543-53. 2006
    ..This better definition of the mechanisms of action of both Tau-1 and Tau-5 is instrumental for the design of alternative therapeutic strategies against prostate cancer...
  19. doi request reprint Identification of androgen-selective androgen-response elements in the human aquaporin-5 and Rad9 genes
    Udo Moehren
    Laboratory of Molecular Endocrinology, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University of Leuven, Campus Gasthuisberg, Onderwijs en Navorsing I, Box 901, Herestraat 49, B 3000 Leuven, Belgium
    Biochem J 411:679-86. 2008
    ..Our data indicate the validity of the hypothesis that selective AREs are recognizable as direct 5'-TGTTCT-3' repeats, and extend the list of currently known selective elements...
  20. pmc The genomic landscape of prostate cancer
    Lien Spans
    Molecular Endocrinology Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, Campus Gasthuisberg, Herestraat 49, P O Box 901, 3000 Leuven, Belgium
    Int J Mol Sci 14:10822-51. 2013
    ..The present review summarizes current knowledge on copy number changes, gene fusions, single nucleotide mutations and polymorphisms, methylation, microRNAs and long non-coding RNAs obtained from high-throughput studies...
  21. pmc Evidence for DNA-binding domain--ligand-binding domain communications in the androgen receptor
    Christine Helsen
    Laboratory of Molecular Endocrinology, Department of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium
    Mol Cell Biol 32:3033-43. 2012
    ..We therefore propose that these residues are involved in allosteric communications between the AR-DBD and AR-LBD...
  22. doi request reprint The hinge region in androgen receptor control
    Liesbeth Clinckemalie
    Molecular Endocrinology Laboratory, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O and N1, Herestraat 49, 3000 Leuven, Belgium
    Mol Cell Endocrinol 358:1-8. 2012
    ..In conclusion, the hinge region serves as an integrator for signals coming from different pathways that provide feedback to the control of AR activity...
  23. doi request reprint Androgen receptor (AR) in osteocytes is important for the maintenance of male skeletal integrity: evidence from targeted AR disruption in mouse osteocytes
    Mieke Sinnesael
    Clinical and Experimental Endocrinology, Department of Experimental Medicine, KU Leuven, Leuven, Belgium
    J Bone Miner Res 27:2535-43. 2012
    ..These findings provide evidence for a direct role of androgens in the maintenance of trabecular bone through actions of the AR in osteocytes...
  24. doi request reprint Inhibition of cathepsin K for treatment of osteoporosis
    Steven Boonen
    Leuven University Division of Geriatric Medicine and Centre for Metabolic Bone Diseases, UZ Leuven Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Curr Osteoporos Rep 10:73-9. 2012
    ..The usefulness of cathepsin K inhibitors in osteoporosis is now being examined in phase 2 and phase 3 clinical trials of postmenopausal osteoporotic women...
  25. pmc Time to onset of antifracture efficacy and year-by-year persistence of effect of zoledronic acid in women with osteoporosis
    Steven Boonen
    University of Leuven, Leuven, Belgium
    J Bone Miner Res 27:1487-93. 2012
    ..097). This analysis shows that zoledronic acid offered significant protection from clinical fractures as early as 12 months. When administered annually, its beneficial effects persisted for at least 3 years...
  26. doi request reprint Androgens and skeletal muscle: cellular and molecular action mechanisms underlying the anabolic actions
    Vanessa Dubois
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, K U Leuven, Campus Gasthuisberg, O and N1, Herestraat 49, Leuven, Belgium
    Cell Mol Life Sci 69:1651-67. 2012
    ..In conclusion, the anabolic effect of androgens on skeletal muscle is not only explained by activation of the myocyte androgen receptor but is also the combined result of many genomic and non-genomic actions...
  27. ncbi request reprint Selective DNA recognition by the androgen receptor as a mechanism for hormone-specific regulation of gene expression
    Guy Verrijdt
    Division of Biochemistry, Laboratory of Biochemistry, Catholic University of Leuven, Campus Gasthuisberg O and N, Herestraat 49, B 3000 Leuven, Belgium
    Mol Genet Metab 78:175-85. 2003
    ..It creates new possibilities in the research on hormone-selective action and provides a new angle in the search for selective ligands or co-factors that might influence androgen receptor action via either type of DNA motif...
  28. ncbi request reprint Squalene synthase, a determinant of Raft-associated cholesterol and modulator of cancer cell proliferation
    Koen Brusselmans
    Laboratory for Experimental Medicine and Endocrinology, Department of Molecular Cell Biology, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    J Biol Chem 282:18777-85. 2007
    ..These findings significantly underscore the importance of de novo cholesterol synthesis for cancer cell biology and suggest that SQS is a potential novel target for antineoplastic intervention...
  29. ncbi request reprint Comparison of different androgen bioassays in the screening for environmental (anti)androgenic activity
    Valerie Christiaens
    Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg, K U Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Environ Toxicol Chem 24:2646-56. 2005
    ..A number of environmental samples were tested in the bioassay. The bioassay also could be used to detect antiandrogenic activity, because known AR-antagonists were able to inhibit R1881-mediated transactivation...
  30. ncbi request reprint The hinge region of the androgen receptor plays a role in proteasome-mediated transcriptional activation
    Tamzin Tanner
    Biochemistry Division, Laboratory of Molecular Endocrinology, Catholic University of Leuven, Campus Gasthuisberg O and N, Herestraat 49, Leuven, 3000, Belgium
    Ann N Y Acad Sci 1030:587-92. 2004
    ....
  31. doi request reprint Novel insights in the regulation and mechanism of androgen action on bone
    Mieke Sinnesael
    Clinical and Experimental Endocrinology, Department of Experimental Medicine, KU Leuven, Leuven, Belgium
    Curr Opin Endocrinol Diabetes Obes 20:240-4. 2013
    ..This review provides an update on the associations of testosterone, estrogens, sex hormone binding globulin, GH-IGF-I, osteocalcin and mechanical loading with relevance to skeletal health...
  32. doi request reprint Identification and characterization of MEL-3, a novel AR antagonist that suppresses prostate cancer cell growth
    Christine Helsen
    Laboratory of Molecular Endocrinology, Department of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium
    Mol Cancer Ther 11:1257-68. 2012
    ....
  33. doi request reprint Once-yearly zoledronic acid in older men compared with women with recent hip fracture
    Steven Boonen
    Center for Musculoskeletal Research, Leuven University, Leuven, Belgium
    J Am Geriatr Soc 59:2084-90. 2011
    ....
  34. doi request reprint Variations in the exome of the LNCaP prostate cancer cell line
    Lien Spans
    Molecular Endocrinology Laboratory, Department of Molecular Cell Biology, University of Leuven, 3000 Leuven, Belgium
    Prostate 72:1317-27. 2012
    ..The LNCaP cell line is widely used as a model for prostate cancer. However, information on protein-changing mutations, genetic heterogeneity and genetic (in)stability is largely lacking for these cells...
  35. pmc Comparative analysis of the influence of the high-mobility group box 1 protein on DNA binding and transcriptional activation by the androgen, glucocorticoid, progesterone and mineralocorticoid receptors
    Guy Verrijdt
    Division of Biochemistry, Faculty of Medicine, Catholic University of Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem J 361:97-103. 2002
    ..This 26-residue fragment is therefore essential for the influence of HMGB1 on DNA recognition by all steroid hormone receptors that were tested. However, it is dispensable for DNA binding by the androgen receptor...
  36. pmc A Sertoli cell-selective knockout of the androgen receptor causes spermatogenic arrest in meiosis
    Karel De Gendt
    Laboratory for Experimental Medicine and Endocrinology, Department of Developmental Biology, Flanders Interuniversity Institute for Biotechnology, Catholic University of Leuven, B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 101:1327-32. 2004
    ..It is concluded that cell-autonomous action of the AR in SC is an absolute requirement for androgen maintenance of complete spermatogenesis, and that spermatocyte/spermatid development/survival critically depends on androgens...
  37. doi request reprint Influence of bone remodelling rate on quantitative ultrasound parameters at the calcaneus and DXA BMDa of the hip and spine in middle-aged and elderly European men: the European Male Ageing Study (EMAS)
    Steven Boonen
    Division of Geriatric Medicine and Centre for Metabolic Bone Diseases, Katholieke Universiteit Leuven, UZ Leuven Campus Gasthuisberg, Leuven, Belgium
    Eur J Endocrinol 165:977-86. 2011
    ..To assess the influence of sex hormones on markers of bone turnover and to explore the association between these markers and bone health in middle-aged and elderly European men...
  38. pmc Structural basis of androgen receptor binding to selective androgen response elements
    Paul L Shaffer
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 101:4758-63. 2004
    ..This increased interfacial stability compared with the other steroid receptors may account for the selective binding of AR to ADR3 response elements...
  39. ncbi request reprint Superagonistic action of 14-epi-analogs of 1,25-dihydroxyvitamin D explained by vitamin D receptor-coactivator interaction
    Guy Eelen
    Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Katholieke Universiteit Leuven, Belgium
    Mol Pharmacol 67:1566-73. 2005
    ..In conclusion, this study provides deeper insight into the docking of TX522 in the LBP and shows that stronger VDR-coactivator interactions underlie the superagonistic activity of the two 14-epi-analogs...
  40. pmc Targeting the BAF57 SWI/SNF subunit in prostate cancer: a novel platform to control androgen receptor activity
    Kevin A Link
    Departments of Cancer Biology and Urology, and Kimmel Cancer Center, Thomas Jefferson University College of Medicine, Philadelphia, Pennsylvania, USA
    Cancer Res 68:4551-8. 2008
    ....