Tine M Bourgois

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Recombinant expression and characterization of XynD from Bacillus subtilis subsp. subtilis ATCC 6051: a GH 43 arabinoxylan arabinofuranohydrolase
    Tine M Bourgois
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, 3001 Leuven, Belgium
    Appl Microbiol Biotechnol 75:1309-17. 2007
  2. ncbi request reprint Targeted molecular engineering of a family 11 endoxylanase to decrease its sensitivity towards Triticum aestivum endoxylanase inhibitor types
    Tine M Bourgois
    Katholieke Universiteit Leuven, Laboratory of Gene Technology, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    J Biotechnol 130:95-105. 2007
  3. doi request reprint Structural analysis of a glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase in complex with xylotetraose reveals a different binding mechanism compared with other members of the same family
    Elien Vandermarliere
    Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49, O and N II, bus 822, 3000 Leuven, Belgium
    Biochem J 418:39-47. 2009
  4. ncbi request reprint Crystallographic analysis shows substrate binding at the -3 to +1 active-site subsites and at the surface of glycoside hydrolase family 11 endo-1,4-beta-xylanases
    Elien Vandermarliere
    Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49, O and N II, bus 822, 3000 Leuven, Belgium
    Biochem J 410:71-9. 2008
  5. pmc Crystallization and preliminary X-ray analysis of an arabinoxylan arabinofuranohydrolase from Bacillus subtilis
    Elien Vandermarliere
    Laboratory for Biocrystallography, Katholieke Universiteit Leuven, Herestraat 49, O and N II bus 822, 3000 Leuven, Belgium
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:692-4. 2007
  6. ncbi request reprint Engineering molecular recognition of endoxylanase enzymes and their inhibitors through phage display
    Tim Beliën
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    J Mol Recognit 20:103-12. 2007
  7. pmc Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis
    Stijn Lagaert
    Katholieke Universiteit Leuven, Division of Gene Technology, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Appl Environ Microbiol 73:5374-7. 2007
  8. ncbi request reprint Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme
    Steven Van Campenhout
    Laboratory of Gene Technology, K U Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Biochem Biophys Res Commun 356:799-804. 2007

Collaborators

Detail Information

Publications8

  1. ncbi request reprint Recombinant expression and characterization of XynD from Bacillus subtilis subsp. subtilis ATCC 6051: a GH 43 arabinoxylan arabinofuranohydrolase
    Tine M Bourgois
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, 3001 Leuven, Belgium
    Appl Microbiol Biotechnol 75:1309-17. 2007
    ..As rXynD did not display endoxylanase, xylosidase or arabinanase activity and was inactive on arabinan, we conclude that this enzyme is best described as an arabinoxylan arabinofuranohydrolase...
  2. ncbi request reprint Targeted molecular engineering of a family 11 endoxylanase to decrease its sensitivity towards Triticum aestivum endoxylanase inhibitor types
    Tine M Bourgois
    Katholieke Universiteit Leuven, Laboratory of Gene Technology, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    J Biotechnol 130:95-105. 2007
    ..Variants from the second strategy showed altered inhibitor sensitivities concomitant with changes in enzyme activities and allowed to gain insight in the binding-mode of both TAXI-I and TAXI-II with BSXY...
  3. doi request reprint Structural analysis of a glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase in complex with xylotetraose reveals a different binding mechanism compared with other members of the same family
    Elien Vandermarliere
    Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49, O and N II, bus 822, 3000 Leuven, Belgium
    Biochem J 418:39-47. 2009
    ..Superposition of the BsAXH-m2,3 structure with known structures of the GH family 43 exo-acting enzymes, beta-xylosidase and alpha-L-arabinanase, each in complex with their substrate, reveals a different orientation of the sugar backbone...
  4. ncbi request reprint Crystallographic analysis shows substrate binding at the -3 to +1 active-site subsites and at the surface of glycoside hydrolase family 11 endo-1,4-beta-xylanases
    Elien Vandermarliere
    Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49, O and N II, bus 822, 3000 Leuven, Belgium
    Biochem J 410:71-9. 2008
    ..subtilis and A. niger xylanases was observed. This extra binding site may have a function similar to the separate carbohydrate-binding modules of other glycoside hydrolase families...
  5. pmc Crystallization and preliminary X-ray analysis of an arabinoxylan arabinofuranohydrolase from Bacillus subtilis
    Elien Vandermarliere
    Laboratory for Biocrystallography, Katholieke Universiteit Leuven, Herestraat 49, O and N II bus 822, 3000 Leuven, Belgium
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:692-4. 2007
    ..Purified recombinant AXH crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 68.7, b = 73.7, c = 106.5 A. X-ray diffraction data were collected to a resolution of 1.55 A...
  6. ncbi request reprint Engineering molecular recognition of endoxylanase enzymes and their inhibitors through phage display
    Tim Beliën
    Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    J Mol Recognit 20:103-12. 2007
    ..In addition, we successfully altered the inhibition susceptibility of the bacterial Bacillus subtilis endoxylanase XynA from XIP-insensitive to XIP-sensitive...
  7. pmc Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis
    Stijn Lagaert
    Katholieke Universiteit Leuven, Division of Gene Technology, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Appl Environ Microbiol 73:5374-7. 2007
    ..Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides...
  8. ncbi request reprint Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme
    Steven Van Campenhout
    Laboratory of Gene Technology, K U Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Biochem Biophys Res Commun 356:799-804. 2007
    ..The implications of these findings in relation to the putative role of the N-terminal propeptide as a carbohydrate binding module and the possible consequences for the way X-I fulfils its role in the germination process, are discussed...