X Bossuyt

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Monoclonal IgM: difficulties with correct measurement
    X Bossuyt
    Department of Laboratory Medicine, University Hospitals Leuven, Belgium
    Ann Clin Biochem 38:708-10. 2001
  2. ncbi request reprint Automated serum protein electrophoresis by Capillarys
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospital Leuven, Leuven, Belgium
    Clin Chem Lab Med 41:704-10. 2003
  3. ncbi request reprint Conflicting results between electrophoresis methods of serum M-proteins
    Birgitte Wuyts
    Department of Clinical Chemistry, University Hospital Ghent, De Pintelaan 185, B 9000 Gent, Belgium
    Electrophoresis 25:1548-50. 2004
  4. ncbi request reprint Laboratory medicine: challenges and opportunities
    Xavier Bossuyt
    Laboratory Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 53:1730-3. 2007
  5. doi request reprint Detection of antinuclear antibodies by automated indirect immunofluorescence analysis
    Xavier Bossuyt
    Experimental Laboratory Immunology, Catholic University Leuven, Leuven, Belgium
    Clin Chim Acta 415:101-6. 2013
  6. pmc Clinical and serological evaluation of a novel CENP-A peptide based ELISA
    Michael Mahler
    Dr Fooke Laboratorien, Mainstrasse 85, 41469 Neuss, Germany
    Arthritis Res Ther 12:R99. 2010
  7. ncbi request reprint Separation of serum proteins by automated capillary zone electrophoresis
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospitals Leuven, Leuven, Belgium
    Clin Chem Lab Med 41:762-72. 2003
  8. ncbi request reprint Reference values for the five electrophoretic serum protein fractions in Caucasian children by capillary zone electrophoresis
    X Bossuyt
    Laboratory Medicine, Immunology, University Hospital, KU Leuven, Belgium
    Clin Chem Lab Med 39:970-2. 2001
  9. ncbi request reprint Novel nephelometric assay for measurement of complement 3d
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospitals Leuven, Belgium
    Ann Clin Biochem 39:34-8. 2002
  10. doi request reprint Clinical performance characteristics of a laboratory test. A practical approach in the autoimmune laboratory
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospital Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Autoimmun Rev 8:543-8. 2009

Collaborators

Detail Information

Publications96

  1. ncbi request reprint Monoclonal IgM: difficulties with correct measurement
    X Bossuyt
    Department of Laboratory Medicine, University Hospitals Leuven, Belgium
    Ann Clin Biochem 38:708-10. 2001
    ..We describe three monoclonal IgM paraproteins for which nephelometric IgM quantification generated inaccurate results...
  2. ncbi request reprint Automated serum protein electrophoresis by Capillarys
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospital Leuven, Leuven, Belgium
    Clin Chem Lab Med 41:704-10. 2003
    ..In conclusion, automated capillary zone electrophoresis with Capillarys provides for reproducible, rapid, and reliable serum electrophoresis...
  3. ncbi request reprint Conflicting results between electrophoresis methods of serum M-proteins
    Birgitte Wuyts
    Department of Clinical Chemistry, University Hospital Ghent, De Pintelaan 185, B 9000 Gent, Belgium
    Electrophoresis 25:1548-50. 2004
    ..5) or pentamerization of IgM M-proteins. Therefore, other causes for discrepancies should be investigated...
  4. ncbi request reprint Laboratory medicine: challenges and opportunities
    Xavier Bossuyt
    Laboratory Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 53:1730-3. 2007
    ....
  5. doi request reprint Detection of antinuclear antibodies by automated indirect immunofluorescence analysis
    Xavier Bossuyt
    Experimental Laboratory Immunology, Catholic University Leuven, Leuven, Belgium
    Clin Chim Acta 415:101-6. 2013
    ..Automated systems for image acquisition and interpretation of indirect immunofluorescence-based tests are increasingly used. The diagnostic performance of such automated approach in untreated patients has not been reported...
  6. pmc Clinical and serological evaluation of a novel CENP-A peptide based ELISA
    Michael Mahler
    Dr Fooke Laboratorien, Mainstrasse 85, 41469 Neuss, Germany
    Arthritis Res Ther 12:R99. 2010
    ..Historically, ACA were detected by indirect immunofluorescence (IIF) on HEp-2 cells and confirmed by immunoassays using recombinant CENP-B. The objective of this study was to evaluate a novel CENP-A peptide ELISA...
  7. ncbi request reprint Separation of serum proteins by automated capillary zone electrophoresis
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospitals Leuven, Leuven, Belgium
    Clin Chem Lab Med 41:762-72. 2003
    ..The reader will be provided with a summation and an understanding of CZE-specific interference...
  8. ncbi request reprint Reference values for the five electrophoretic serum protein fractions in Caucasian children by capillary zone electrophoresis
    X Bossuyt
    Laboratory Medicine, Immunology, University Hospital, KU Leuven, Belgium
    Clin Chem Lab Med 39:970-2. 2001
    ..We report age-related reference intervals for capillary zone electrophoresis for children between 1 and 14 years of age...
  9. ncbi request reprint Novel nephelometric assay for measurement of complement 3d
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospitals Leuven, Belgium
    Ann Clin Biochem 39:34-8. 2002
    ..This report describes a nephelometric assay for measuring complement 3d (C3d)...
  10. doi request reprint Clinical performance characteristics of a laboratory test. A practical approach in the autoimmune laboratory
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospital Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Autoimmun Rev 8:543-8. 2009
    ..This review aims to make laboratory professionals and clinicians more familiar with the basic concepts that deal with the clinical performance characteristics of a laboratory test...
  11. ncbi request reprint Serologic markers in inflammatory bowel disease
    Xavier Bossuyt
    Laboratory Medicine, University Hospitals Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Clin Chem 52:171-81. 2006
    ..Pancreatic antibodies and newly identified anti-microbial antibodies (anti-outer membrane porin C, anti-I2, and anti-flagellin) are also reviewed...
  12. ncbi request reprint Interferences in clinical capillary zone electrophoresis of serum proteins
    Xavier Bossuyt
    Laboratory Medicine Immunology, University Hospitals Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Electrophoresis 25:1485-7. 2004
    ..This minireview gives a survey of interfering substances, including iodinated contrast agents and antibiotics...
  13. ncbi request reprint Differential regulation of UDP-GlcUA transport in endoplasmic reticulum and in Golgi membranes
    X Bossuyt
    Department of Molecular Cell Biology, Catholic University of Leuven, Belgium
    J Hepatol 34:210-4. 2001
    ..Intramicrosomal UDP-GlcNAc stimulates influx of UDP-GlcUA and thereby enhances delivery of UDP-GlcUA to the catalytic center of UGT in the ER lumen...
  14. ncbi request reprint Detection of anti-SSA antibodies by indirect immunofluorescence
    Xavier Bossuyt
    Laboratory Medicine and Internal Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 50:2361-9. 2004
    ..We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA...
  15. ncbi request reprint Serum protein electrophoresis by CZE 2000 clinical capillary electrophoresis system
    X Bossuyt
    Department of Clinical Pathology, University Hospital of Leuven, Belgium
    Clin Chem 44:749-59. 1998
    ..We conclude that CZE offers a clinically reliable alternative to AGE and CAE and has the advantages of automation, higher precision, and faster turnaround time...
  16. ncbi request reprint Detection and classification of paraproteins by capillary immunofixation/subtraction
    X Bossuyt
    Department of Clinical Pathology, University Hospital of Leuven, Belgium
    Clin Chem 44:760-4. 1998
    ..The sensitivity for detection and identification of oligoclonal banding by capillary electrophoresis was inferior to immunofixation...
  17. ncbi request reprint Serum protein electrophoresis and immunofixation by a semiautomated electrophoresis system
    X Bossuyt
    Department of Clinical Pathology, University Hospital of Leuven, Belgium
    Clin Chem 44:944-9. 1998
    ..In the other cases, no differences between the manual methods and the semiautomated method were seen with respect to paraprotein identification...
  18. ncbi request reprint Evaluation of two automated enzyme immunoassays for detection of antinuclear antibodies
    X Bossuyt
    Department of Laboratory Medicine, University Hospital Leuven, Belgium
    Clin Chem Lab Med 38:1033-7. 2000
    ....
  19. ncbi request reprint Evaluation of interferences in rate and fixed-time nephelometric assays of specific serum proteins
    X Bossuyt
    Department of Clinical Pathology, University Hospital Leuven, Kapucijnenvoer 33, B 3000 Leuven, Belgium
    Clin Chem 45:62-7. 1999
    ..This effect was more pronounced with the Beckman reagent than with the Behring reagent in all three nephelometers and was independent of the type of instrument (fixed-time vs rate nephelometry)...
  20. ncbi request reprint Screening for autoantibodies to SS-A/RO by indirect immunofluorescence using HEp-2000 cells
    X Bossuyt
    Laboratory of Medicine, University Hospital Leuven, The Netherlands
    Ann Clin Biochem 37:216-9. 2000
    ..In conclusion, the presence of the typical 'distinctive' SS-A pattern on IF using the HEp-2000 slides is highly specific for the presence of autoantibodies to SS-A and has a sensitivity of 88% for detecting these antibodies...
  21. ncbi request reprint Diagnostic value of anti-Saccharomyces cerevisiae and antineutrophil cytoplasmic autoantibodies in inflammatory bowel disease
    M Peeters
    Department of Human Genetics, University Hospital Gasthuisberg, Leuven, Belgium
    Am J Gastroenterol 96:730-4. 2001
    ..The combination of tests is probably more powerful, although, clinical subgroups still need to be defined. The usefulness of these markers in indeterminate colitis needs to be studied prospectively...
  22. ncbi request reprint Anti-Saccharomyces cerevisiae antibodies (ASCA), phenotypes of IBD, and intestinal permeability: a study in IBD families
    S Vermeire
    Gastroenterology Unit, UZ Gasthuisberg, Leuven, Belgium
    Inflamm Bowel Dis 7:8-15. 2001
    ..The association is much weaker in patients belonging to mixed families. ASCA is a stable marker and is not a secondary phenomenon due to increased intestinal permeability...
  23. pmc Uridine diphosphoxylose enhances hepatic microsomal UDP-glucuronosyltransferase activity by stimulating transport of UDP-glucuronic acid across the endoplasmic reticulum membrane
    X Bossuyt
    Laboratory of Biological Chemistry, Department of Molecular Cell Biology, Catholic University of Leuven, Belgium
    Biochem J 315:189-93. 1996
    ....
  24. ncbi request reprint Comparative study of ASCA (Anti-Saccharomyces cerevisiae antibody) assays in inflammatory bowel disease
    S Vermeire
    Gastroenterology Unit, UZ Gasthuisberg, Leuven, Belgium
    Gastroenterology 120:827-33. 2001
    ..Although agreement between and within assays is good, caution is important when absolute values are used. Standardization of ASCA measurements is greatly needed...
  25. pmc Diagnostic characteristics of a gelatin based Waaler-Rose assay (Serodia-RA) for the detection of rheumatoid factor
    T Spiritus
    Department of Laboratory Medicine, University Hospitals Leuven, Belgium
    Ann Rheum Dis 63:1169-71. 2004
    ..To evaluate the diagnostic usefulness of a gelatin based Waaler Rose assay (Serodia-RA) for the detection of rheumatoid factor (RF) in rheumatoid arthritis (RA)...
  26. doi request reprint Anti-PM/Scl-100 and anti-RNA-polymerase III antibodies in scleroderma
    L Maes
    Laboratory Medicine, Immunology, University Hospitals Leuven, Leuven, Belgium
    Clin Chim Acta 411:965-71. 2010
    ..Recently, new ELISA based immunoassays have become available for the detection of anti-PM/Scl and anti-RNA Pol-lII antibodies...
  27. ncbi request reprint [The effects of physical exercise on the immune system]
    A Jeurissen
    Afd Experimentele Laboratoriumgeneeskunde, Universitair Ziekenhuis Gasthuisberg, Herestraat 49, 3000 Leuven, Belgie
    Ned Tijdschr Geneeskd 147:1347-51. 2003
    ..Glutamine and vitamin C could, hypothetically, prevent the negative effects of strenuous exercise on the immune function, but further studies are needed to demonstrate and explain these effects...
  28. doi request reprint (Auto)antibodies in inflammatory bowel diseases
    Severine Vermeire
    Department of Gastroenterology, University Hospitals Leuven Belgium, UZ Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Gastroenterol Clin North Am 37:429-38, vii. 2008
    ..However, currently, they have no place in the diagnostic work up. Their real promise may lie in their use as surrogate markers of complicated aggressive disease as shown in various retrospective studies, but prospective data are lacking...
  29. ncbi request reprint Clonal proliferation of T-Cell large granular lymphocytes
    Nancy Boeckx
    Department of Laboratory Medicine, Laboratory of Haematology, University Hospital Gasthuisberg, Leuven, Belgium
    Pediatr Blood Cancer 42:275-7. 2004
    ..We diagnosed a new case of a clonal T-LGL proliferation in a 6-year-old girl presenting with severe neutropenia and pure red blood cell aplasia (PRCA)...
  30. ncbi request reprint Clinical relevance of measurement of antibodies to individual snU1-RNP proteins
    Ariane Luyckx
    Laboratory Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 51:1888-90. 2005
  31. ncbi request reprint Quantification of IgG antibodies to Aspergillus fumigatus and pigeon antigens by ImmunoCAP technology: an alternative to the precipitation technique?
    Erna Van Hoeyveld
    Department of Laboratory Medicine, Immunology, University Hospital Leuven, Belgium
    Clin Chem 52:1785-93. 2006
    ..We evaluated the ImmunoCAP technique for measurement of IgG specific to Aspergillus fumigatus and pigeon antigens...
  32. ncbi request reprint Detection of single nucleotide polymorphisms in the mannose-binding lectin gene using minor groove binder-DNA probes
    Erna Van Hoeyveld
    Department of Laboratory Medicine, Immunology, Gasthuisberg University Hospital, Herestraat 49, B 3000 Leuven, Belgium
    J Immunol Methods 287:227-30. 2004
    ..In conclusion, the 5' nuclease assay using MGB probes is useful for large-scale screening of point mutations/polymorphisms, even when they are in close proximity...
  33. doi request reprint Evaluation of a panel of 28 biomarkers for the non-invasive diagnosis of endometriosis
    A Vodolazkaia
    Leuven University Fertility Centre, Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium
    Hum Reprod 27:2698-711. 2012
    ....
  34. ncbi request reprint Comparative study of two cytofluorometric methods of analysis
    C Wouters
    Department of Paediatrics, University Hospital Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium
    J Immunol Methods 234:89-98. 2000
    ..Moreover, the consistency of the measurements is better. A major disadvantage for statistical analysis, however, is that the Attractor program has not been adapted for non-parametric data...
  35. ncbi request reprint The value of serologic markers in indeterminate colitis: a prospective follow-up study
    Sofie Joossens
    Gastroenterology Unit, Department of Pathology, U Z Gasthuisberg, Leuven, Belgium
    Gastroenterology 122:1242-7. 2002
    ..We evaluated the value of anti-Saccharomyces cerevisiae antibodies (ASCA) and perinuclear antineutrophil cytoplasmic antibodies (pANCA) to increase diagnostic accuracy in categorizing IC...
  36. ncbi request reprint Pancreatic autoantibodies in inflammatory bowel disease
    Sofie Joossens
    Department of Gastroenterology, University Hospital Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium
    Inflamm Bowel Dis 10:771-7. 2004
    ..We studied PABs in patients with inflammatory bowel disease (IBD), unaffected family members, and control subjects...
  37. ncbi request reprint Human memory B lymphocyte subsets fulfill distinct roles in the anti-polysaccharide and anti-protein immune response
    Leen Moens
    Experimental Laboratory Medicine, Department of Medical Diagnostic Sciences, Group Biomedical Sciences, Catholic University, Leuven, Belgium
    J Immunol 181:5306-12. 2008
    ..pneumoniae or soluble pneumococcal capsular polysaccharides. In conclusion, our findings provide evidence for a versatile role of IgM memory B cells in T-independent and T-dependent immune responses...
  38. ncbi request reprint Coexistence of (partial) immune defects and risk of recurrent respiratory infections
    Xavier Bossuyt
    Department of Laboratory Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 53:124-30. 2007
    ..We studied whether and to what extent immunologic and genetic factors are associated with increased susceptibility to respiratory infections...
  39. ncbi request reprint Advances in serum protein electrophoresis
    Xavier Bossuyt
    Laboratory Medicine, Immunology, University Hospitals Leuven, Herestraat 9, B 3000 Leuven, Belgium
    Adv Clin Chem 42:43-80. 2006
    ..Examples of electrophoretic patterns are given...
  40. doi request reprint Determination of anti-neutrophil cytoplasmic antibodies in small vessel vasculitis: Comparative analysis of different strategies
    Pieter Vermeersch
    Laboratory Medicine, Immunology, University Hospitals Leuven, Belgium
    Clin Chim Acta 397:77-81. 2008
    ..We evaluated whether a strategy that is based on screening with ELISA or fluoroenzymeimmunoassay (FEIA) is an accurate alternative to screening with indirect immunofluorescence (IIF)...
  41. doi request reprint Heterogeneous nuclear ribonucleoprotein h1, a novel nuclear autoantigen
    Karolien Van den Bergh
    Laboratory Medicine, Immunology, University Hospitals Leuven, Leuven, Belgium
    Clin Chem 55:946-54. 2009
    ....
  42. ncbi request reprint Isolated IgG3 deficiency in children: to treat or not to treat? Case presentation and review of the literature
    Isabelle Meyts
    Department of Pediatrics, University Hospital Leuven, Herestraat 49, Leuven, Belgium
    Pediatr Allergy Immunol 17:544-50. 2006
    ..The currently available literature on IgG3 deficiency is reviewed with specific emphasis on the peculiarities of the IgG3 subclass, the clinical relevance of IgG3 deficiency as well as the therapeutic options...
  43. doi request reprint Immune dysfunction in patients with functional gastrointestinal disorders
    S Kindt
    Department of Gastroenterological Research, KU Leuven, Leuven, Belgium
    Neurogastroenterol Motil 21:389-98. 2009
    ..A shift towards a Th2 cytokine profile is present in FGID, while the cellular immunophenotype remains largely unchanged. Further research is indicated and could provide new therapeutic strategies for these disorders...
  44. ncbi request reprint Evaluation of the immune response to pneumococcal capsular polysaccharides
    A Jeurissen
    Afdeling Experimentele Laboratoriumgeneeskunde, Departement Moleculaire Celbiologie, Katholieke Universiteit Leuven, Herestraat 49, 3000 Leuven, Belgium
    Acta Clin Belg 58:106-10. 2003
    ..In this article we describe the indications, the method used to measure antibodies to capsular pneumococcal polysaccharide and the way the results have to be interpreted...
  45. ncbi request reprint Contribution of genetic and environmental factors in the pathogenesis of Crohn's disease in a large family with multiple cases
    Marie Joossens
    Department of Gastroenterology, University Hospital, Leuven, Belgium
    Inflamm Bowel Dis 13:580-4. 2007
    ..In this family the father developed Crohn's disease (CD) after moving from Morocco to Belgium and successively 4 of his 8 children subsequently developed CD. There was no previous history of familial CD...
  46. ncbi request reprint Partial loss of presenilins causes seborrheic keratosis and autoimmune disease in mice
    Jos Tournoy
    Neuronal Cell Biology Laboratory, Center for Human Genetics CB 4, University Hospital, Catholic University of Leuven, Belgium
    Hum Mol Genet 13:1321-31. 2004
    ..Skin and immune system appear to be especially sensitive targets of impaired PS function and may need careful monitoring if gamma-secretase inhibitors are envisaged for treating AD...
  47. doi request reprint Diagnostic performance of IgG anti-deamidated gliadin peptide antibody assays is comparable to IgA anti-tTG in celiac disease
    Pieter Vermeersch
    Laboratory Medicine, Immunology, University Hospitals Leuven, Catholic University of Leuven, Leuven, Belgium
    Clin Chim Acta 411:931-5. 2010
    ..Our aim was to evaluate the technical performance and diagnostic accuracy of four commercial IgG anti-DGP assays...
  48. doi request reprint Diagnostic performance of serum free light chain measurement in patients suspected of a monoclonal B-cell disorder
    Pieter Vermeersch
    Department of Laboratory Medicine, University Hospital, Leuven, Catholic University of Leuven, Leuven, Belgium
    Br J Haematol 143:496-502. 2008
    ..The sensitivity of serum IFE plus FLC was comparable to the sensitivity of serum IFE plus urine IFE. The specificity of serum IFE plus FLC, however, was lower than the specificity of serum IFE plus urine IFE...
  49. ncbi request reprint Interassay and interobserver variability in the detection of anti-neutrophil cytoplasmic antibodies in patients with ulcerative colitis
    Sofie Joossens
    Department of Gastroenterology, University Hospital Gasthuisberg, KULeuven, Leuven, Belgium
    Clin Chem 50:1422-5. 2004
  50. pmc Generation of antibody responses to pneumococcal capsular polysaccharides is independent of CD1 expression in mice
    Leen Moens
    University Hospital Leuven, Laboratory Medicine Immunology, Campus Gasthuisberg, CDG 7th Floor, Herestraat 49, B 3000 Leuven, Belgium
    Infect Immun 77:1976-80. 2009
    ..In summary, our data provide evidence that the antibody response to caps-PS is generated independently of CD1 expression...
  51. ncbi request reprint Detection of monoclonal proteins in sera by capillary zone electrophoresis and free light chain measurements
    Godelieve Mariën
    Laboratory Medicine, Immunology, University Hospitals Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Clin Chem 48:1600-1. 2002
  52. pmc Specific intracellular adhesion molecule-grabbing nonintegrin R1 is not involved in the murine antibody response to pneumococcal polysaccharides
    Leen Moens
    Department of Medical Diagnostic Sciences, Laboratory of Experimental Laboratory Medicine, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium
    Infect Immun 75:5748-52. 2007
    ..There is no direct relation between capture and uptake of caps-PS serotype 14 by Sign-R1 and the initiation of the anti-caps-PS antibody production in mice...
  53. doi request reprint Use of interval-specific likelihood ratios improves clinical interpretation of serum FLC results for the diagnosis of malignant plasma cell disorders
    Pieter Vermeersch
    Laboratory Medicine, Immunology, University Hospitals Leuven, Katholieke Universiteit Leuven, Herestraat 49, 3000 Leuven, Belgium
    Clin Chim Acta 410:54-8. 2009
    ..We examined whether the use of test result interval-specific likelihood ratios (LR) could improve the clinical interpretation of serum FLC kappa/lambda ratio for the diagnosis of malignant plasma cell disorders...
  54. ncbi request reprint Distinct approaches to investigate the importance of the murine 4-1BB 4-1BBL interaction in the antibody response to Streptococcus pneumoniae
    Leen Moens
    Department of Molecular Cell Biology, Faculty of Medicine, Catholic University Leuven, University Hospital Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
    J Leukoc Biol 82:638-44. 2007
    ..The 4-1BB-4-1BBL interaction is not involved in the antibody response to soluble caps-PS. The influence of the 4-1BB-4-1BBL interaction in the immune reaction to S. pneumoniae Serotype 3 depends on the experimental system used...
  55. ncbi request reprint Diagnostic accuracy of ten second-generation (human) tissue transglutaminase antibody assays in celiac disease
    Britta Van Meensel
    Laboratory Medicine, Immunology, Internal Medicine, Gastroenterology, Department of Paediatrics, Department of Pathology, University Hospital, Leuven, Belgium
    Clin Chem 50:2125-35. 2004
    ..We evaluated commercially available assays with human tTG antigen to estimate their diagnostic accuracies and to determine whether they agree sufficiently to be used interchangeably...
  56. ncbi request reprint 1alpha,25-Dihydroxyvitamin D3 modulates the murine antibody response to pneumococcal capsular polysaccharide serotype 3 through IL-12
    Axel Jeurissen
    Experimental Laboratory Medicine, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium
    Eur J Immunol 35:1841-8. 2005
    ..In conclusion, we demonstrate that 1alpha,25(OH)(2)D(3) has an inhibitory effect on the IgG2a antibody response to caps-PS serotype 3, and that this effect was mediated trough IL-12...
  57. ncbi request reprint Autoimmunity associated with anti-tumor necrosis factor alpha treatment in Crohn's disease: a prospective cohort study
    Severine Vermeire
    Gastroenterology Department, University Hospital Gasthiuisberg, Leuven, Belgium
    Gastroenterology 125:32-9. 2003
    ..We investigated the occurrence of antinuclear antibodies in 125 consecutive Crohn's disease patients and studied the relationship with symptoms of autoimmunity...
  58. pmc T lymphocyte dependence of the antibody response to 'T lymphocyte independent type 2' antigens
    A Jeurissen
    Experimental Laboratory Medicine, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium
    Immunology 111:1-7. 2004
  59. ncbi request reprint A critical appraisal of current practice in the detection, analysis, and reporting of cryoglobulins
    Pieter Vermeersch
    Department of Laboratory Medicine, Immunology, University Hospitals Leuven, Catholic University of Leuven, Herestraat 49, Leuven, Belgium
    Clin Chem 54:39-43. 2008
    ..The serum should cryoprecipitate at 4 degrees C for at least 3 (preferably 7) days. The cryoprecipitate should be washed and resolubilized at 37 degrees C for further analysis...
  60. ncbi request reprint Technical and diagnostic performance of 6 assays for the measurement of citrullinated protein/peptide antibodies in the diagnosis of rheumatoid arthritis
    Dries Coenen
    Departments of Laboratory Medicine and Immunology, University Hospital Leuven, Belgium
    Clin Chem 53:498-504. 2007
    ..We evaluated the technical performance and diagnostic accuracy of 6 ELISAs for the detection of antibodies to citrullinated protein/peptide antigens...
  61. doi request reprint Establishment of reference values for immunoglobulins in the cryoprecipitate
    Pieter Vermeersch
    Department of Laboratory Medicine, Immunology, University Hospitals Leuven, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Clin Immunol 129:360-4. 2008
    ..Complement C4 was decreased in 61% and 55% of the patients classified as type II and type III compared to 16% of the patients that were negative for cryoglobulins and 9% of the diseased controls...
  62. pmc Hyper-immunoglobulin M syndrome caused by a mutation in the promotor for CD40L
    Erna Van Hoeyveld
    Laboratory Medicine, Immunology, University Hospital Leuven, Belgium
    Immunology 120:497-501. 2007
    ..The mutation in the promotor region likely contributed to the decreased transcription as demonstrated by a luciferase reporter assay...
  63. ncbi request reprint Blockade of CTLA-4 (CD152) enhances the murine antibody response to pneumococcal capsular polysaccharides
    Michael Boudewijns
    Experimental Laboratory Medicine, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University Leuven, Belgium
    J Leukoc Biol 78:1060-9. 2005
    ..Taken together, these data strongly suggest a role of T lymphocytes and CTLA-4 in the regulation of the antibody response to caps-PS...
  64. doi request reprint Anti-alpha-enolase antibodies in patients with inflammatory Bowel disease
    Nathalie Vermeulen
    Department of Internal Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 54:534-41. 2008
    ..alpha-Enolase has been proposed as a target antigen in IBD. We evaluated the prevalence and diagnostic value of anti-alpha-enolase antibodies in IBD and related disorders...
  65. ncbi request reprint Essential role for CD40 ligand interactions in T lymphocyte-mediated modulation of the murine immune response to pneumococcal capsular polysaccharides
    Axel Jeurissen
    Department of Molecular Cell Biology, Laboratory of Experimental Laboratory Medicine, Catholic University Leuven, Leuven, Belgium
    J Immunol 168:2773-81. 2002
    ..In summary, our data provide evidence that T lymphocytes contribute to the regulation of the anti-caps-PS immune response in a CD40L-dependent manner...
  66. ncbi request reprint Fcgamma-receptor IIA genotype and invasive pneumococcal infection
    Leen Moens
    Department of Laboratory Medicine, University Hospital Leuven, Centraal Dienstengebouw, Herestraat 49, B 3000 Leuven, Belgium
    Clin Immunol 118:20-3. 2006
    ..In contrast to previous reports, we found no association between Fcgamma-RIIA genotype and invasive pneumococcal disease...
  67. doi request reprint Laboratory diagnosis of specific antibody deficiency to pneumococcal capsular polysaccharide antigens by multiplexed bead assay
    Heleen Borgers
    Experimental Laboratory Medicine, Department of Medical Diagnostic Sciences, Biomedical Sciences Group, Catholic University Leuven, Leuven, Belgium
    Clin Immunol 134:198-205. 2010
    ..The XMAP pneumococcal immunity panel appears useful for identifying individuals with a low response to the unconjugated pneumococcal vaccine...
  68. doi request reprint Use of likelihood ratios improves clinical interpretation of IgA anti-tTG antibody testing for celiac disease
    Pieter Vermeersch
    Laboratory Medicine, Immunology, University Hospital Gasthuisberg, Catholic University of Leuven, Belgium
    Clin Chim Acta 411:13-7. 2010
    ..We investigated whether taking into account IgA anti-tissue transglutaminase antibody concentration (IgA anti-tTG) and total IgA concentration could improve clinical interpretation of serologic testing for celiac disease (CD)...
  69. ncbi request reprint The human antibody response to pneumococcal capsular polysaccharides is dependent on the CD40-CD40 ligand interaction
    Axel Jeurissen
    Experimental Laboratory Medicine, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium
    Eur J Immunol 34:850-8. 2004
    ..This is the first demonstration that the human immune response to caps-PS, which is markedly regulated by T lymphocytes, is dependent on the CD40-CD40L interaction...
  70. ncbi request reprint Mannose-binding lectin genotype and invasive pneumococcal infection
    Leen Moens
    Department of Laboratory Medicine, CDG, University Hospital Leuven, Leuven, Belgium
    Hum Immunol 67:605-11. 2006
    ..On the other hand, the -221 and -550 promoter allele distribution and the prevalence of the combined MBL structural and promoter -221 variant alleles were not significantly different between the patient group and the control group...
  71. ncbi request reprint National external quality assessment scheme for lymphocyte immunophenotyping in Belgium
    Marian Van Blerk
    Department of Clinical Biology, Scientific Institute of Public Health, Brussels, Belgium
    Clin Chem Lab Med 41:323-30. 2003
    ..Between-laboratory variability remained stable over time. Analysis of individual laboratory performance indicated that some laboratories markedly improved their results...
  72. ncbi request reprint Can heparin plasma be used instead of serum for nephelometric analysis of serum proteins?
    Mieke Develter
    Clin Chem 52:1609-10. 2006
  73. ncbi request reprint Effect of sulfamethoxazole on clinical capillary zone electrophoresis of serum proteins
    Xavier Bossuyt
    Clin Chem 49:340-1. 2003
  74. ncbi request reprint Determination of IgG subclasses: a need for standardization
    Xavier Bossuyt
    J Allergy Clin Immunol 115:872-4. 2005
  75. ncbi request reprint Role of ASCA and the NOD2/CARD15 mutation Gly908Arg in predicting increased surgical costs in Crohn's disease patients: a project of the European Collaborative Study Group on Inflammatory Bowel Disease
    Shmuel Odes
    Gastroenterology and Hepatology Department, Soroka Hospital and Ben Gurion University of Negev, Beer Sheva, Israel
    Inflamm Bowel Dis 13:874-81. 2007
    ..We hypothesized that the presence of ASCA and NOD2/CARD15 mutations could predict increased health care cost in CD...
  76. ncbi request reprint Effect of piperacillin-tazobactam on clinical capillary zone electrophoresis of serum proteins
    Xavier Bossuyt
    Clin Chem 48:204-5. 2002
  77. doi request reprint Standardisation in clinical laboratory medicine: an ethical reflection
    Xavier Bossuyt
    Ann Rheum Dis 67:1061-3. 2008
  78. ncbi request reprint Ethanol precipitation is not reliable for selectively removing nonmonoclonal peaks seen in the fibrinogen region on capillary zone electrophoresis of serum proteins
    Koenraad Gijbels
    Clin Chem 50:1880-1. 2004
  79. ncbi request reprint Clinical capillary zone electrophoresis of serum proteins: balancing high sensitivity and high specificity
    Godelieve Mariën
    Clin Chem 49:1419-20; discussion 1420-1. 2003
  80. ncbi request reprint Interference by gelatin-based plasma substitutes in capillary zone electrophoresis
    Koenraad Gijbels
    Clin Chem 50:1473-5. 2004
  81. ncbi request reprint Detection of heavy chain disease by capillary zone electrophoresis
    Godelieve Mariën
    Clin Chem 51:1302-3. 2005
  82. ncbi request reprint Antibodies to extractable nuclear antigens in antinuclear antibody-negative samples
    Xavier Bossuyt
    Clin Chem 51:2426-7. 2005
  83. ncbi request reprint Antinuclear antibody titer and antibodies to extractable nuclear antigens
    Xavier Bossuyt
    Arthritis Rheum 53:987-8. 2005
  84. ncbi request reprint Successful allogeneic hemopoietic stem cell transplantation in a child who had anhidrotic ectodermal dysplasia with immunodeficiency
    Sophie Dupuis-Girod
    Unité d Immunologie et d Hématologie Pédiatriques, Institut National de la Santé et de la Recherche Médicale U429, Hopital Necker Enfants Malades, Paris, France
    Pediatrics 118:e205-11. 2006
    ..Hematopoietic stem cell transplantation is well tolerated and efficiently cures the profound immunodeficiency associated with autosomal-dominant anhidrotic ectodermal dysplasia with immunodeficiency...
  85. ncbi request reprint Interference of sulfamethoxazole in Capillarys electrophoresis
    Annelies Brouwers
    Clin Chem Lab Med 44:910-1. 2006
  86. ncbi request reprint Towards zero prevalence of chronic Pseudomonas aeruginosa infection in children with cystic fibrosis
    Patrick Lebecque
    Department of Pediatrics and Clinical Chemistry, St Luc University Hospital, Universite Catholique de Louvain, 1200 Brussels, Belgium
    J Cyst Fibros 5:237-44. 2006
    ..Data from the Belgian Cystic Fibrosis Registry consistently show that in one of the seven reference centres, the prevalence of Pseudomonas aeruginosa is half that observed at the national level...
  87. ncbi request reprint Free light chain testing in follow-up of multiple myeloma
    Marjan Van Gysel
    Clin Chem Lab Med 44:1044-6. 2006
  88. pmc IRAK4 and NEMO mutations in otherwise healthy children with recurrent invasive pneumococcal disease
    Cheng Lung Ku
    Laboratory of Human Genetics of Infectious Diseases, Necker Medical School, University of Paris Rene Descartes, Paris, France
    J Med Genet 44:16-23. 2007
    ..About 2% of childhood episodes of invasive pneumococcal disease (IPD) are recurrent, and most remain unexplained...
  89. ncbi request reprint T cell-dependent and -independent responses
    Axel Jeurissen
    J Immunol 172:2728; author reply 2728-9. 2004
  90. ncbi request reprint A case of pseudoparaproteinemia on capillary zone electrophoresis caused by geloplasma
    Pieter Vermeersch
    Clin Chem 52:2309-11. 2006
  91. ncbi request reprint Pseudoparaproteinemia related to iomeprol administration after angiocardiography: Detection in the beta fraction by capillary zone electrophoresis
    Pieter Vermeersch
    Clin Chem 52:2312-3. 2006
  92. ncbi request reprint Detection of monoclonal proteins by capillary zone electrophoresis: comparison of 2 multichannel automated systems
    Xavier Bossuyt
    Clin Chem 53:152-3. 2007
  93. ncbi request reprint Interference of ceftriaxone on capillary zone electrophoresis
    Annelies Brouwers
    Clin Chim Acta 376:255-6. 2007
  94. ncbi request reprint Response to the comments of K. Day and J. Zakowski on "Clinical capillary zone electrophoresis of serum proteins: balancing high sensitivity and high specificity"
    Godelieve Mariën
    Clin Chem 49:1711-2. 2003
  95. ncbi request reprint Cellular and humoral aberrations in a kindred with IL-1 receptor-associated kinase 4 deficiency
    Elana Lavine
    J Allergy Clin Immunol 120:948-50. 2007
  96. ncbi request reprint False-negative serum protein electrophoresis in a sample with an IgM monoclonal protein by semiautomated gel electrophoresis
    Xavier Bossuyt
    Clin Chem 51:270. 2005