Monique Beullens

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. pmc CDK2 regulates HIV-1 transcription by phosphorylation of CDK9 on serine 90
    Denitra Breuer
    Center for Sickle Cell Disease, Department of Medicine, Howard University, 1840 7th Street, N W HURB1, Suite 202, Washington, DC 20001, USA
    Retrovirology 9:94. 2012
  2. ncbi request reprint Substrate specificity and activity regulation of protein kinase MELK
    Monique Beullens
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 280:40003-11. 2005
  3. ncbi request reprint Interactor-mediated nuclear translocation and retention of protein phosphatase-1
    Bart Lesage
    Division of Biochemistry, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    J Biol Chem 279:55978-84. 2004
  4. ncbi request reprint Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1
    Veerle Vulsteke
    Afdeling Biochemie, Faculteit Geneeskunde, Catholic University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 279:8642-7. 2004
  5. ncbi request reprint A complex of catalytically inactive protein phosphatase-1 sandwiched between Sds22 and inhibitor-3
    Bart Lesage
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University of Leuven, B 3000 Leuven, Belgium
    Biochemistry 46:8909-19. 2007
  6. ncbi request reprint Nucleocytoplasmic shuttling of the splicing factor SIPP1
    Miriam Llorian
    Afdelingen Biochemie, Faculteit Geneeskunde, KULeuven, B 3000 Leuven, Belgium
    J Biol Chem 280:38862-9. 2005
  7. ncbi request reprint Degeneracy and function of the ubiquitous RVXF motif that mediates binding to protein phosphatase-1
    Paulina Wakula
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 278:18817-23. 2003
  8. doi request reprint Nature of the nuclear inclusions formed by PQBP1, a protein linked to neurodegenerative polyglutamine diseases
    Emilia Nicolaescu
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, Campus Gasthuisberg, O and N1 Box901, Herestraat 49, B 3000 Leuven, Belgium
    Eur J Cell Biol 87:817-29. 2008
  9. ncbi request reprint The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor
    Qiming Jin
    Division of Biochemistry, Faculty of Medicine, University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 278:30677-85. 2003
  10. ncbi request reprint Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1
    An Boudrez
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:31834-41. 2002

Collaborators

Detail Information

Publications32

  1. pmc CDK2 regulates HIV-1 transcription by phosphorylation of CDK9 on serine 90
    Denitra Breuer
    Center for Sickle Cell Disease, Department of Medicine, Howard University, 1840 7th Street, N W HURB1, Suite 202, Washington, DC 20001, USA
    Retrovirology 9:94. 2012
    ..We also found that inhibition of CDK2 by iron chelators leads to the inhibition of CDK9 activity, suggesting a functional link between CDK2 and CDK9. Here, we investigate whether CDK2 phosphorylates CDK9 and regulates its activity...
  2. ncbi request reprint Substrate specificity and activity regulation of protein kinase MELK
    Monique Beullens
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 280:40003-11. 2005
    ..A C-terminal fragment of MELK functions as an autoinhibitory domain. Our data show that the activity of MELK is regulated in a complex manner and offer new perspectives for the further elucidation of its biological function...
  3. ncbi request reprint Interactor-mediated nuclear translocation and retention of protein phosphatase-1
    Bart Lesage
    Division of Biochemistry, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    J Biol Chem 279:55978-84. 2004
    ..Our data suggest that both the nuclear translocation and the nuclear retention of PP1 depend on its binding to interactors with an RVXF motif...
  4. ncbi request reprint Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1
    Veerle Vulsteke
    Afdeling Biochemie, Faculteit Geneeskunde, Catholic University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 279:8642-7. 2004
    ..Our data suggest that MELK has a role in the cell cycle-regulated control of pre-mRNA splicing...
  5. ncbi request reprint A complex of catalytically inactive protein phosphatase-1 sandwiched between Sds22 and inhibitor-3
    Bart Lesage
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University of Leuven, B 3000 Leuven, Belgium
    Biochemistry 46:8909-19. 2007
    ..Therefore, our studies show that a pool of PP1 is complexly controlled by both Sds22 and I3...
  6. ncbi request reprint Nucleocytoplasmic shuttling of the splicing factor SIPP1
    Miriam Llorian
    Afdelingen Biochemie, Faculteit Geneeskunde, KULeuven, B 3000 Leuven, Belgium
    J Biol Chem 280:38862-9. 2005
    ..These findings also have potential implications for a better understanding of the pathogenesis of X-linked mental retardation and polyglutamine-linked neurodegenerative disorders...
  7. ncbi request reprint Degeneracy and function of the ubiquitous RVXF motif that mediates binding to protein phosphatase-1
    Paulina Wakula
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 278:18817-23. 2003
    ....
  8. doi request reprint Nature of the nuclear inclusions formed by PQBP1, a protein linked to neurodegenerative polyglutamine diseases
    Emilia Nicolaescu
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, Campus Gasthuisberg, O and N1 Box901, Herestraat 49, B 3000 Leuven, Belgium
    Eur J Cell Biol 87:817-29. 2008
    ..Our data suggest that the SIPP1-PQBP1-induced nuclear inclusions are distinct from the protein aggregates that are associated with polyglutamine diseases and represent dynamic nucleoplasmic heteropolymers of SIPP1 and PQBP1...
  9. ncbi request reprint The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor
    Qiming Jin
    Division of Biochemistry, Faculty of Medicine, University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 278:30677-85. 2003
    ..Also, the histone deacetylase 2 was present in a complex with NIPP1. Our data are in accordance with a role for NIPP1 as a DNA-targeting protein for EED and associated chromatin-modifying enzymes...
  10. ncbi request reprint Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1
    An Boudrez
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:31834-41. 2002
    ..We discuss how the interaction between NIPP1 and SAP155 could contribute to spliceosome (dis)assembly and the catalytic steps of splicing...
  11. pmc NIPP1 maintains EZH2 phosphorylation and promoter occupancy at proliferation-related target genes
    Nikki Minnebo
    Laboratory of Biosignaling and Therapeutics, Department of Cellular and Molecular Medicine, KU Leuven, B 3000 Leuven, Belgium
    Nucleic Acids Res 41:842-54. 2013
    ..Our data identify PP1 as an EZH2 phosphatase and demonstrate that the phosphorylation-regulated association of EZH2 with proliferation-related targets depends on associated NIPP1...
  12. pmc The translation initiation factor eIF2beta is an interactor of protein phosphatase-1
    Paulina Wakula
    Department of Molecular Cell Biology, Division of Biochemistry, Faculty of Medicine, Katholieke Universiteit Leuven, B3000 Leuven, Belgium
    Biochem J 400:377-83. 2006
    ..The overexpression of wild-type eIF2beta or eIF2beta with a mutated RVxF-motif did not differentially affect the rate of translation, indicating that the binding of PP1 is not rate-limiting for translation under basal conditions...
  13. pmc The phosphatase interactor NIPP1 regulates the occupancy of the histone methyltransferase EZH2 at Polycomb targets
    Nele Van Dessel
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, KULeuven, B 3000 Leuven, Belgium
    Nucleic Acids Res 38:7500-12. 2010
    ..We propose that NIPP1 associates with a subset of PcG targets in a PP1-dependent manner and thereby contributes to the recruitment of the PRC2 complex...
  14. pmc Protein phosphatase-1 is a novel regulator of the interaction between IRBIT and the inositol 1,4,5-trisphosphate receptor
    Benoit Devogelaere
    Laboratory of Molecular and Cellular Signalling, Department of Molecular Cell Biology, Katholieke Universiteit Leuven, Campus Gasthuisberg O N1, B 3000 Leuven, Belgium
    Biochem J 407:303-11. 2007
    ..Finally, we have shown that mutational inactivation of the docking site for PP1 on IRBIT increased the affinity of IRBIT for the IP3R. This pinpoints PP1 as a key player in the regulation of IP3R-controlled Ca2+ signals...
  15. ncbi request reprint Glucose activates a protein phosphatase-1-mediated signaling pathway to enhance overall translation in pancreatic beta-cells
    Dirk Vander Mierde
    Gene Expression Unit, Department of Molecular Cell Biology, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Endocrinology 148:609-17. 2007
    ..Because defects in the pancreatic endoplasmic reticulum kinase-eIF2alpha signaling system lead to beta-cell failure and diabetes, deregulation of the PP1 system could likewise lead to cellular dysfunction and disease...
  16. doi request reprint Docking motif-guided mapping of the interactome of protein phosphatase-1
    Annick Hendrickx
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Chem Biol 16:365-71. 2009
    ..Notably, an interaction network linking PP1 interactors discloses a pleiotropic role of PP1 in cell polarity...
  17. doi request reprint PP1/Repo-man dephosphorylates mitotic histone H3 at T3 and regulates chromosomal aurora B targeting
    Junbin Qian
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
    Curr Biol 21:766-73. 2011
    ..Consistent with these findings, Repo-Man modulates in a PP1-dependent manner the H3T3ph-regulated chromosomal targeting of Aurora kinase B and its substrate MCAK. Our study defines a novel mechanism by which PP1 counteracts Aurora B...
  18. pmc The extended PP1 toolkit: designed to create specificity
    Mathieu Bollen
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
    Trends Biochem Sci 35:450-8. 2010
    ..These novel insights offer perspectives for the therapeutic targeting of PP1 by interfering with the binding of PIPs or substrates...
  19. ncbi request reprint The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly
    Monique Beullens
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:19855-60. 2002
    ..Our data suggest that NIPP1 can function independently as a splicing factor and a phosphatase regulator...
  20. ncbi request reprint False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise
    Monique Beullens
    Department of Molecular Cell Biology, Catholic University of Leuven, Belgium
    Blood 107:4711-3. 2006
    ..We show here that this widely used test can occasionally lead to the false-positive detection of rhEpo (epoetin-beta) in postexercise, protein-rich urine, probably because the adopted monoclonal anti-Epo antibodies are not monospecific...
  21. ncbi request reprint Characterization of the condensin component Cnap1 and protein kinase Melk as novel E2F target genes down-regulated by 1,25-dihydroxyvitamin D3
    Lieve Verlinden
    Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Katholieke Universiteit Leuven, Belgium
    J Biol Chem 280:37319-30. 2005
    ....
  22. ncbi request reprint Protein phosphatase-1 dephosphorylates the C-terminal domain of RNA polymerase-II
    Kareem Washington
    Center for Sickle Cell Disease, Department of Biochemistry and Molecular Biology, Howard University, 2121 Georgia Avenue, Washington, D C 20059, USA
    J Biol Chem 277:40442-8. 2002
    ..Our data demonstrate that RNAPII CTD is dephosphorylated by PP1 in vitro and by PPP-type phosphatase, distinct from FCP1, in vivo...
  23. pmc Functional interaction between nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1) in Drosophila: consequences of over-expression of NIPP1 in flies and suppression by co-expression of PP1
    Louise Parker
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Biochem J 368:789-97. 2002
    ..These data show that NIPP1Dm and PP1c interact in vivo and suggest that NIPP1's role as a phosphatase regulator is conserved in Drosophila...
  24. ncbi request reprint AKAP149 is a novel PP1 specifier required to maintain nuclear envelope integrity in G1 phase
    Rikke L Steen
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    J Cell Sci 116:2237-46. 2003
    ..This correlates with the induction of a G1 arrest and, ultimately, apoptosis. We propose that AKAP149-regulated PP1 activity at the NE during G1 is required to maintain nuclear integrity and cell survival...
  25. pmc SIPP1, a novel pre-mRNA splicing factor and interactor of protein phosphatase-1
    Miriam Llorian
    Departamento de Biologia Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad Asociada al CIB CSIC, 39011 Santander, Spain
    Biochem J 378:229-38. 2004
    ..Finally, we found that SIPP1 is also a component of the spliceosomes and that a SIPP1-fragment inhibits splicing catalysis by nuclear extracts independent of its ability to interact with PP1...
  26. ncbi request reprint Regulation of protein phosphatase 1gamma activity in hypoxia through increased interaction with NIPP1: implications for cellular metabolism
    Kathrina M Comerford
    School of Medicine and Medical Science, UCD Conway Institute, University College Dublin, Ireland
    J Cell Physiol 209:211-8. 2006
    ....
  27. ncbi request reprint Nuclear targeting of protein phosphatase-1 by HIV-1 Tat protein
    Tatyana Ammosova
    Center for Sickle Cell Disease, Howard University, Washington, DC 20059, USA
    J Biol Chem 280:36364-71. 2005
    ..Our results suggest that Tat might function as a nuclear regulator of PP1 and that interaction of Tat with PP1 is critical for activation of HIV-1 transcription by Tat...
  28. ncbi request reprint Testing for recombinant erythropoietin
    Joris R Delanghe
    Department of Clinical Chemistry, University Hospital, Ghent, Belgium
    Am J Hematol 83:237-41. 2008
    ..Indirect tests have the disadvantage that they require blood instead of urine samples, but they can be applied to detect a broader range of performance improving techniques which are illicitly used in sports...
  29. ncbi request reprint Nuclear protein phosphatase-1 regulates HIV-1 transcription
    Tatyana Ammosova
    Center for Sickle Cell Disease and Department of Biochemistry and Molecular Biology, Howard University, Washington, D C 20059, USA
    J Biol Chem 278:32189-94. 2003
    ..Our results indicate that PP1 might be a host cell factor that is required for HIV-1 viral transcription. Therefore, nuclear PP1 may represent a novel target for anti-HIV-1 therapeutics...
  30. ncbi request reprint The KH-Tudor domain of a-kinase anchoring protein 149 mediates RNA-dependent self-association
    Marie Rogne
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, P O Box 1112 Blindern, 0317 Oslo, Norway
    Biochemistry 45:14980-9. 2006
    ..AKAP149 emerges as a scaffolding protein involved in the integration of intracellular signals and possibly in RNA metabolism...
  31. ncbi request reprint A SERCA2 pump with an increased Ca2+ affinity can lead to severe cardiac hypertrophy, stress intolerance and reduced life span
    Peter Vangheluwe
    Laboratory of Physiology, University of Leuven, Belgium
    J Mol Cell Cardiol 41:308-17. 2006
    ..An improved Ca(2+) transport in the sarcoplasmic reticulum may correct Ca(2+) mishandling in heart failure, but a SERCA pump with a much higher Ca(2+) affinity may be detrimental...
  32. pmc The nuclear scaffold protein NIPP1 is essential for early embryonic development and cell proliferation
    Aleyde Van Eynde
    Division of Biochemistry, Center for Transgene Technology and Gene Therapy, Flanders Interuniversity, Institute for Biotechnology, Leuven, Belgium
    Mol Cell Biol 24:5863-74. 2004
    ..We conclude that NIPP1 is indispensable for early embryonic development and cell proliferation...