Research Topics
Genomes and Genes
| Monique BeullensSummaryAffiliation: Katholieke Universiteit Leuven Country: Belgium Publications
| Collaborators
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Detail Information
Publications
Substrate specificity and activity regulation of protein kinase MELKMonique Beullens
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
J Biol Chem 280:40003-11. 2005..A C-terminal fragment of MELK functions as an autoinhibitory domain. Our data show that the activity of MELK is regulated in a complex manner and offer new perspectives for the further elucidation of its biological function...- Interactor-mediated nuclear translocation and retention of protein phosphatase-1Bart Lesage
Division of Biochemistry, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
J Biol Chem 279:55978-84. 2004..Our data suggest that both the nuclear translocation and the nuclear retention of PP1 depend on its binding to interactors with an RVXF motif... - Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1Veerle Vulsteke
Afdeling Biochemie, Faculteit Geneeskunde, Catholic University of Leuven, B 3000 Leuven, Belgium
J Biol Chem 279:8642-7. 2004..Our data suggest that MELK has a role in the cell cycle-regulated control of pre-mRNA splicing... - A complex of catalytically inactive protein phosphatase-1 sandwiched between Sds22 and inhibitor-3Bart Lesage
Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University of Leuven, B 3000 Leuven, Belgium
Biochemistry 46:8909-19. 2007..Therefore, our studies show that a pool of PP1 is complexly controlled by both Sds22 and I3... - Nucleocytoplasmic shuttling of the splicing factor SIPP1Miriam Llorian
Afdelingen Biochemie, Faculteit Geneeskunde, KULeuven, B 3000 Leuven, Belgium
J Biol Chem 280:38862-9. 2005..These findings also have potential implications for a better understanding of the pathogenesis of X-linked mental retardation and polyglutamine-linked neurodegenerative disorders... - Degeneracy and function of the ubiquitous RVXF motif that mediates binding to protein phosphatase-1Paulina Wakula
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
J Biol Chem 278:18817-23. 2003.... 
Nature of the nuclear inclusions formed by PQBP1, a protein linked to neurodegenerative polyglutamine diseasesEmilia Nicolaescu
Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, Campus Gasthuisberg, O and N1 Box901, Herestraat 49, B 3000 Leuven, Belgium
Eur J Cell Biol 87:817-29. 2008..Our data suggest that the SIPP1-PQBP1-induced nuclear inclusions are distinct from the protein aggregates that are associated with polyglutamine diseases and represent dynamic nucleoplasmic heteropolymers of SIPP1 and PQBP1...- The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressorQiming Jin
Division of Biochemistry, Faculty of Medicine, University of Leuven, B 3000 Leuven, Belgium
J Biol Chem 278:30677-85. 2003..Also, the histone deacetylase 2 was present in a complex with NIPP1. Our data are in accordance with a role for NIPP1 as a DNA-targeting protein for EED and associated chromatin-modifying enzymes... - Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1An Boudrez
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
J Biol Chem 277:31834-41. 2002..We discuss how the interaction between NIPP1 and SAP155 could contribute to spliceosome (dis)assembly and the catalytic steps of splicing... 
The translation initiation factor eIF2beta is an interactor of protein phosphatase-1Paulina Wakula
Department of Molecular Cell Biology, Division of Biochemistry, Faculty of Medicine, Katholieke Universiteit Leuven, B3000 Leuven, Belgium
Biochem J 400:377-83. 2006..The overexpression of wild-type eIF2beta or eIF2beta with a mutated RVxF-motif did not differentially affect the rate of translation, indicating that the binding of PP1 is not rate-limiting for translation under basal conditions...- The phosphatase interactor NIPP1 regulates the occupancy of the histone methyltransferase EZH2 at Polycomb targetsNele Van Dessel
Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, KULeuven, B 3000 Leuven, Belgium
Nucleic Acids Res 38:7500-12. 2010..We propose that NIPP1 associates with a subset of PcG targets in a PP1-dependent manner and thereby contributes to the recruitment of the PRC2 complex... - Protein phosphatase-1 is a novel regulator of the interaction between IRBIT and the inositol 1,4,5-trisphosphate receptorBenoit Devogelaere
Laboratory of Molecular and Cellular Signalling, Department of Molecular Cell Biology, Katholieke Universiteit Leuven, Campus Gasthuisberg O N1, B 3000 Leuven, Belgium
Biochem J 407:303-11. 2007..Finally, we have shown that mutational inactivation of the docking site for PP1 on IRBIT increased the affinity of IRBIT for the IP3R. This pinpoints PP1 as a key player in the regulation of IP3R-controlled Ca2+ signals... - Glucose activates a protein phosphatase-1-mediated signaling pathway to enhance overall translation in pancreatic beta-cellsDirk Vander Mierde
Gene Expression Unit, Department of Molecular Cell Biology, KU Leuven, Herestraat 49, B 3000 Leuven, Belgium
Endocrinology 148:609-17. 2007..Because defects in the pancreatic endoplasmic reticulum kinase-eIF2alpha signaling system lead to beta-cell failure and diabetes, deregulation of the PP1 system could likewise lead to cellular dysfunction and disease... - Docking motif-guided mapping of the interactome of protein phosphatase-1Annick Hendrickx
Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
Chem Biol 16:365-71. 2009..Notably, an interaction network linking PP1 interactors discloses a pleiotropic role of PP1 in cell polarity... - PP1/Repo-man dephosphorylates mitotic histone H3 at T3 and regulates chromosomal aurora B targetingJunbin Qian
Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
Curr Biol 21:766-73. 2011..Consistent with these findings, Repo-Man modulates in a PP1-dependent manner the H3T3ph-regulated chromosomal targeting of Aurora kinase B and its substrate MCAK. Our study defines a novel mechanism by which PP1 counteracts Aurora B... - The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assemblyMonique Beullens
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
J Biol Chem 277:19855-60. 2002..Our data suggest that NIPP1 can function independently as a splicing factor and a phosphatase regulator... - The extended PP1 toolkit: designed to create specificityMathieu Bollen
Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
Trends Biochem Sci 35:450-8. 2010..These novel insights offer perspectives for the therapeutic targeting of PP1 by interfering with the binding of PIPs or substrates... - False-positive detection of recombinant human erythropoietin in urine following strenuous physical exerciseMonique Beullens
Department of Molecular Cell Biology, Catholic University of Leuven, Belgium
Blood 107:4711-3. 2006..We show here that this widely used test can occasionally lead to the false-positive detection of rhEpo (epoetin-beta) in postexercise, protein-rich urine, probably because the adopted monoclonal anti-Epo antibodies are not monospecific... - Characterization of the condensin component Cnap1 and protein kinase Melk as novel E2F target genes down-regulated by 1,25-dihydroxyvitamin D3Lieve Verlinden
Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Katholieke Universiteit Leuven, Belgium
J Biol Chem 280:37319-30. 2005.... - Nuclear targeting of protein phosphatase-1 by HIV-1 Tat proteinTatyana Ammosova
Center for Sickle Cell Disease, Howard University, Washington, DC 20059, USA
J Biol Chem 280:36364-71. 2005..Our results suggest that Tat might function as a nuclear regulator of PP1 and that interaction of Tat with PP1 is critical for activation of HIV-1 transcription by Tat... - Protein phosphatase-1 dephosphorylates the C-terminal domain of RNA polymerase-IIKareem Washington
Center for Sickle Cell Disease, Department of Biochemistry and Molecular Biology, Howard University, 2121 Georgia Avenue, Washington, D C 20059, USA
J Biol Chem 277:40442-8. 2002..Our data demonstrate that RNAPII CTD is dephosphorylated by PP1 in vitro and by PPP-type phosphatase, distinct from FCP1, in vivo... - Functional interaction between nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1) in Drosophila: consequences of over-expression of NIPP1 in flies and suppression by co-expression of PP1Louise Parker
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
Biochem J 368:789-97. 2002..These data show that NIPP1Dm and PP1c interact in vivo and suggest that NIPP1's role as a phosphatase regulator is conserved in Drosophila... - AKAP149 is a novel PP1 specifier required to maintain nuclear envelope integrity in G1 phaseRikke L Steen
Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
J Cell Sci 116:2237-46. 2003..This correlates with the induction of a G1 arrest and, ultimately, apoptosis. We propose that AKAP149-regulated PP1 activity at the NE during G1 is required to maintain nuclear integrity and cell survival... - SIPP1, a novel pre-mRNA splicing factor and interactor of protein phosphatase-1Miriam Llorian
Departamento de Biologia Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad Asociada al CIB CSIC, 39011 Santander, Spain
Biochem J 378:229-38. 2004..Finally, we found that SIPP1 is also a component of the spliceosomes and that a SIPP1-fragment inhibits splicing catalysis by nuclear extracts independent of its ability to interact with PP1... - Regulation of protein phosphatase 1gamma activity in hypoxia through increased interaction with NIPP1: implications for cellular metabolismKathrina M Comerford
School of Medicine and Medical Science, UCD Conway Institute, University College Dublin, Ireland
J Cell Physiol 209:211-8. 2006.... - Nuclear protein phosphatase-1 regulates HIV-1 transcriptionTatyana Ammosova
Center for Sickle Cell Disease and Department of Biochemistry and Molecular Biology, Howard University, Washington, D C 20059, USA
J Biol Chem 278:32189-94. 2003..Our results indicate that PP1 might be a host cell factor that is required for HIV-1 viral transcription. Therefore, nuclear PP1 may represent a novel target for anti-HIV-1 therapeutics... - The KH-Tudor domain of a-kinase anchoring protein 149 mediates RNA-dependent self-associationMarie Rogne
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, P.O. Box 1112 Blindern, 0317 Oslo, Norway
Biochemistry 45:14980-9. 2006..AKAP149 emerges as a scaffolding protein involved in the integration of intracellular signals and possibly in RNA metabolism... - A SERCA2 pump with an increased Ca2+ affinity can lead to severe cardiac hypertrophy, stress intolerance and reduced life spanPeter Vangheluwe
Laboratory of Physiology, University of Leuven, Belgium
J Mol Cell Cardiol 41:308-17. 2006..An improved Ca(2+) transport in the sarcoplasmic reticulum may correct Ca(2+) mishandling in heart failure, but a SERCA pump with a much higher Ca(2+) affinity may be detrimental... - The nuclear scaffold protein NIPP1 is essential for early embryonic development and cell proliferationAleyde Van Eynde
Division of Biochemistry, Center for Transgene Technology and Gene Therapy, Flanders Interuniversity, Institute for Biotechnology, Leuven, Belgium
Mol Cell Biol 24:5863-74. 2004..We conclude that NIPP1 is indispensable for early embryonic development and cell proliferation... - Testing for recombinant erythropoietinJoris R Delanghe
Department of Clinical Chemistry, University Hospital, Ghent, Belgium
Am J Hematol 83:237-41. 2008..Indirect tests have the disadvantage that they require blood instead of urine samples, but they can be applied to detect a broader range of performance improving techniques which are illicitly used in sports...