Ambroos Stals

Summary

Affiliation: Institute for Agricultural and Fisheries Research
Country: Belgium

Publications

  1. ncbi request reprint Laboratory efforts to eliminate contamination problems in the real-time RT-PCR detection of noroviruses
    Ambroos Stals
    Flemish Government, Institute for Agricultural and Fisheries Research, Unit Technology and Food, Melle, Belgium
    J Microbiol Methods 77:72-6. 2009
  2. ncbi request reprint Molecular detection and genotyping of noroviruses
    Ambroos Stals
    Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Ghent University, Coupure Links 653, 9000, Ghent, Belgium
    Food Environ Virol 4:153-67. 2012
  3. ncbi request reprint Extraction of food-borne viruses from food samples: a review
    Ambroos Stals
    Institute for Agricultural and Fisheries Research ILVO, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium
    Int J Food Microbiol 153:1-9. 2012
  4. ncbi request reprint Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 1
    Ambroos Stals
    Flemish Government, Institute for Agricultural and Fisheries Research ILVO, Technology and Food Sciences Unit, Oost Vlaanderen, Belgium
    J Virol Methods 161:247-53. 2009
  5. ncbi request reprint Screening of fruit products for norovirus and the difficulty of interpreting positive PCR results
    Ambroos Stals
    Laboratory of Food Microbiology and Food Preservation, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium
    J Food Prot 74:425-31. 2011
  6. ncbi request reprint Norovirus transfer between foods and food contact materials
    Ambroos Stals
    Flanders Government, Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium
    J Food Prot 76:1202-9. 2013
  7. ncbi request reprint Evaluation of a norovirus detection methodology for ready-to-eat foods
    Ambroos Stals
    Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure Links 653, 9000 Ghent, Belgium
    Int J Food Microbiol 145:420-5. 2011
  8. ncbi request reprint Evaluation of viral concentration methods from irrigation and processing water
    Ann De Keuckelaere
    Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
    J Virol Methods 187:294-303. 2013
  9. ncbi request reprint Evaluation of a norovirus detection methodology for soft red fruits
    Ambroos Stals
    Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Ghent, Belgium
    Food Microbiol 28:52-8. 2011
  10. ncbi request reprint The need for harmonization in detection of human noroviruses in food
    Ambroos Stals
    Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure Links 653, 9000 Ghent, Belgium
    J AOAC Int 96:998-1005. 2013

Detail Information

Publications14

  1. ncbi request reprint Laboratory efforts to eliminate contamination problems in the real-time RT-PCR detection of noroviruses
    Ambroos Stals
    Flemish Government, Institute for Agricultural and Fisheries Research, Unit Technology and Food, Melle, Belgium
    J Microbiol Methods 77:72-6. 2009
    ..In general, our results showed that the high sensitivity of an optimized real-time PCR assay should be considered as--besides a great advantage--a potential risk factor for obtaining false-positive results when using this technique...
  2. ncbi request reprint Molecular detection and genotyping of noroviruses
    Ambroos Stals
    Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Ghent University, Coupure Links 653, 9000, Ghent, Belgium
    Food Environ Virol 4:153-67. 2012
    ....
  3. ncbi request reprint Extraction of food-borne viruses from food samples: a review
    Ambroos Stals
    Institute for Agricultural and Fisheries Research ILVO, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium
    Int J Food Microbiol 153:1-9. 2012
    ..This review summarizes these virus extraction approaches and the results obtained from published protocols. The use of process controls is also briefly described...
  4. ncbi request reprint Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 1
    Ambroos Stals
    Flemish Government, Institute for Agricultural and Fisheries Research ILVO, Technology and Food Sciences Unit, Oost Vlaanderen, Belgium
    J Virol Methods 161:247-53. 2009
    ..In addition, a collection of bovine NoVs and other (non-NoV) enteric viruses were found to be negative, and no cross-amplification between genogroups was observed...
  5. ncbi request reprint Screening of fruit products for norovirus and the difficulty of interpreting positive PCR results
    Ambroos Stals
    Laboratory of Food Microbiology and Food Preservation, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium
    J Food Prot 74:425-31. 2011
    ..These results, however, may indicate a prior NoV contamination of the tested food samples throughout the fresh produce chain...
  6. ncbi request reprint Norovirus transfer between foods and food contact materials
    Ambroos Stals
    Flanders Government, Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium
    J Food Prot 76:1202-9. 2013
    ..4. The obtained transfer efficiency data may provide insights into the transfer of NoV during preparation of foods and can be included in risk assessment models describing the transmission of NoVs in this context. ..
  7. ncbi request reprint Evaluation of a norovirus detection methodology for ready-to-eat foods
    Ambroos Stals
    Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure Links 653, 9000 Ghent, Belgium
    Int J Food Microbiol 145:420-5. 2011
    ..In conclusion, the evaluated NoV detection method was capable of detecting NoV in RTE foods, although recoveries were influenced by the inoculum level and by the food type...
  8. ncbi request reprint Evaluation of viral concentration methods from irrigation and processing water
    Ann De Keuckelaere
    Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
    J Virol Methods 187:294-303. 2013
    ..Mean recovery efficiencies ranging from 4.8% for detection of GI NoV in open well water to 32.1% for detection of HAV in bore hole water, depending on the water type and the viral pathogen analyzed...
  9. ncbi request reprint Evaluation of a norovirus detection methodology for soft red fruits
    Ambroos Stals
    Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Ghent, Belgium
    Food Microbiol 28:52-8. 2011
    ....
  10. ncbi request reprint The need for harmonization in detection of human noroviruses in food
    Ambroos Stals
    Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure Links 653, 9000 Ghent, Belgium
    J AOAC Int 96:998-1005. 2013
    ..Harmonization may be a first step to comprehend and deal with these limitations. The current review provides an overview of a number of validated methods that have been published by food safety and other authorities...
  11. ncbi request reprint Viral genes everywhere: public health implications of PCR-based testing of foods
    Ambroos Stals
    Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure Links 653, 9000 Ghent, Belgium
    Curr Opin Virol 3:69-73. 2013
    ..Finally, the current review provides insights in a selection of methods currently under development that may be able to facilitate the specific detection of infectious food borne viruses...
  12. ncbi request reprint Batch testing for noroviruses in frozen raspberries
    Ann De Keuckelaere
    Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium Electronic address
    Int J Food Microbiol 192:43-50. 2015
    ..It was shown that the applied protocol for sequencing of the amplicon to confirm the specificity of the RT-qPCR signal was successful for GI NoV amplicons but often failed and provided an inconclusive result for GII NoV amplicons. ..
  13. ncbi request reprint Semi-direct lysis of swabs and evaluation of their efficiencies to recover human noroviruses GI and GII from surfaces
    Ann De Keuckelaere
    Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium
    Food Environ Virol 6:132-9. 2014
    ..4 and GII.4 NoVs were detected between the three different swabs. For the coarser NR, biowipes turned out to be the best option for detecting both GI.4 and GII.4 NoV. ..
  14. ncbi request reprint Detection of noroviruses in shellfish and semiprocessed fishery products from a Belgian seafood company
    Dan Li
    Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    J Food Prot 77:1342-7. 2014
    ....