Anandi Martin

Summary

Affiliation: Institute of Tropical Medicine
Country: Belgium

Publications

  1. pmc Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis
    Juan Carlos Palomino
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium
    Antimicrob Agents Chemother 46:2720-2. 2002
  2. ncbi request reprint One-tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish
    Roman Kotlowski
    Prince Leopold Institute of Tropical Medicine, Mycobacteriology Unit, Nationalestraat 155, B 2000 Antwerpen, Belgium
    J Med Microbiol 53:927-33. 2004
  3. pmc Evaluation of direct detection of Mycobacterium tuberculosis rifampin resistance by a nitrate reductase assay applied to sputum samples in Cotonou, Benin
    Dissou Affolabi
    Laboratoire de Référence des Mycobactéries, BP 817 Cotonou Bénin
    J Clin Microbiol 45:2123-5. 2007
  4. ncbi request reprint Application of the hsp65 PRA method for the rapid identification of mycobacteria isolated from clinical samples in Belgium
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp, B 2000 Belgium
    J Microbiol Methods 71:39-43. 2007
  5. ncbi request reprint Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates
    A Martin
    Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium
    Int J Tuberc Lung Dis 13:1301-4. 2009
  6. pmc Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in Mycobacterium tuberculosis
    Sergio Luis Yzquierdo
    Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    Ann Clin Microbiol Antimicrob 5:11. 2006
  7. pmc Rapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    J Clin Microbiol 46:3952-6. 2008
  8. ncbi request reprint A new rapid and simple colorimetric method to detect pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine Nationalestraat 155, Antwerpen, B 2000 Belgium
    J Antimicrob Chemother 58:327-31. 2006
  9. ncbi request reprint Multicenter study of MTT and resazurin assays for testing susceptibility to first-line anti-tuberculosis drugs
    A Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    Int J Tuberc Lung Dis 9:901-6. 2005
  10. ncbi request reprint Nitrate reductase assay for the rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium
    J Antimicrob Chemother 61:123-7. 2008

Collaborators

Detail Information

Publications42

  1. pmc Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis
    Juan Carlos Palomino
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium
    Antimicrob Agents Chemother 46:2720-2. 2002
    ..Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs...
  2. ncbi request reprint One-tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish
    Roman Kotlowski
    Prince Leopold Institute of Tropical Medicine, Mycobacteriology Unit, Nationalestraat 155, B 2000 Antwerpen, Belgium
    J Med Microbiol 53:927-33. 2004
    ..ulcerans infection...
  3. pmc Evaluation of direct detection of Mycobacterium tuberculosis rifampin resistance by a nitrate reductase assay applied to sputum samples in Cotonou, Benin
    Dissou Affolabi
    Laboratoire de Référence des Mycobactéries, BP 817 Cotonou Bénin
    J Clin Microbiol 45:2123-5. 2007
    ..NRA is simple to perform and provides a rapid, accurate, and cost-effective means for the detection of rifampin resistance in M. tuberculosis isolates...
  4. ncbi request reprint Application of the hsp65 PRA method for the rapid identification of mycobacteria isolated from clinical samples in Belgium
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp, B 2000 Belgium
    J Microbiol Methods 71:39-43. 2007
    ..PRA was found useful in the identification of mycobacteria and simple to implement as a quick method in the laboratory...
  5. ncbi request reprint Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates
    A Martin
    Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium
    Int J Tuberc Lung Dis 13:1301-4. 2009
    ..In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics...
  6. pmc Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in Mycobacterium tuberculosis
    Sergio Luis Yzquierdo
    Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    Ann Clin Microbiol Antimicrob 5:11. 2006
    ..Phage assay have been reported as a rapid useful tools for antimicrobial susceptibility testing. The aim of this study was to apply the Phage assay for rapid detection of resistance on Mycobacterium tuberculosis strains in Cuba...
  7. pmc Rapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    J Clin Microbiol 46:3952-6. 2008
    ..tuberculosis to second-line drugs. There is no need for a machine when using the manual MGIT, and results can be read with a simple UV lamp or with a semiquantitative reader, which considerably reduces the cost of the method...
  8. ncbi request reprint A new rapid and simple colorimetric method to detect pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine Nationalestraat 155, Antwerpen, B 2000 Belgium
    J Antimicrob Chemother 58:327-31. 2006
    ..The purpose of this study was to develop and assess a rapid method for pyrazinamide resistance detection in Mycobacterium tuberculosis using nicotinamide in a colorimetric resazurin assay...
  9. ncbi request reprint Multicenter study of MTT and resazurin assays for testing susceptibility to first-line anti-tuberculosis drugs
    A Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    Int J Tuberc Lung Dis 9:901-6. 2005
    ..5-diphenyltetrazolium bromide) and resazurin assays, for testing the susceptibility of Mycobacterium tuberculosis to the first-line anti-tuberculosis drugs rifampicin (RMP), isoniazid (INH), ethambutol (EMB) and streptomycin (SM)...
  10. ncbi request reprint Nitrate reductase assay for the rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium
    J Antimicrob Chemother 61:123-7. 2008
    ....
  11. ncbi request reprint Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp, B 2000, Belgium
    J Microbiol Methods 63:145-50. 2005
    ..6% and 97.9%, respectively. For streptomycin, discrepant results were obtained with an overall accuracy of 85.3%. The assay proved easy to be implemented in countries with limited laboratory facilities...
  12. pmc Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp B 2000, Belgium
    J Clin Microbiol 43:1612-6. 2005
    ..Nitrate reductase and resazurin assays are rapid, simple, low-cost methods and might become inexpensive alternative procedures for rapid detection of OFX resistance in low-resource countries...
  13. doi request reprint Evaluation of the BD MGIT TBc Identification Test (TBc ID), a rapid chromatographic immunoassay for the detection of Mycobacterium tuberculosis complex from liquid culture
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    J Microbiol Methods 84:255-7. 2011
    ..tuberculosis complex in liquid medium...
  14. doi request reprint Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    J Antimicrob Chemother 66:827-33. 2011
    ..To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin...
  15. doi request reprint The nitrate reductase assay for the rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    J Antimicrob Chemother 62:56-64. 2008
    ..The nitrate reductase assay (NRA) has been proposed as a rapid method for the detection of resistance to rifampicin and isoniazid, but its accuracy has not been systematically evaluated...
  16. doi request reprint Evaluation of the TB Ag MPT64 Rapid test for the identification of Mycobacterium tuberculosis complex
    A Martin
    Institute of Tropical Medicine, Antwerp, Belgium
    Int J Tuberc Lung Dis 15:703-5. 2011
    ..5% and 100% compared to the polymerase chain reaction. The overall concordance of the TB Ag MPT64 Rapid test was 969%. The TB Ag MPT64 Rapid test is easy, sensitive, and does not require a high level of skill or specific equipment...
  17. pmc Implementation of the thin layer agar method for diagnosis of smear-negative pulmonary tuberculosis in a setting with a high prevalence of human immunodeficiency virus infection in Homa Bay, Kenya
    Anandi Martin
    Institute of Tropical Medicine, Antwerp, Belgium
    J Clin Microbiol 47:2632-4. 2009
    ..The sensitivities of LJ and TLA were 71% and 74%, respectively. TLA could become an affordable method for the diagnosis of smear-negative tuberculosis in resource-limited settings, with results available within 2 weeks...
  18. doi request reprint Thin layer agar compared to BACTEC MGIT 960 for early detection of Mycobacterium tuberculosis
    Anandi Martin
    Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium
    J Microbiol Methods 78:107-8. 2009
    ..The sensitivity of detection of M. tuberculosis was 97.3% on TLA and 97% on BACTEC MGIT960 for smear positive samples. TLA showed comparable results to BACTEC MGIT960 and could be an alternative method for low-income countries...
  19. pmc Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium
    Antimicrob Agents Chemother 47:3616-9. 2003
    ..The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries...
  20. ncbi request reprint Colorimetric redox-indicator methods for the rapid detection of multidrug resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine Nationalestraat 155, 2000 Antwerp, Belgium
    J Antimicrob Chemother 59:175-83. 2007
    ..These methods appear as promising new tools but their accuracy has not been systematically evaluated...
  21. doi request reprint hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis in comparison to three other methods for identification of Mycobacterium species
    Anna Sajduda
    Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B 2000 Antwerp, Belgium
    J Microbiol Methods 80:190-7. 2010
    ..Also, PRA identified seven isolates representing five previously unreported hsp65 alleles. We conclude that hsp65 PRA based on automated capillary electrophoresis is a rapid, simple and reliable method for identification of mycobacteria...
  22. doi request reprint Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis
    Juan Carlos Palomino
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium
    J Microbiol Methods 75:161-6. 2008
    ..This review will address phenotypic culture-based methods for rapid drug susceptibility testing in M. tuberculosis...
  23. doi request reprint hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis for species identification and differentiation of Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group
    Anna Sajduda
    Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B 2000 Antwerp, Belgium
    Int J Infect Dis 16:e193-7. 2012
    ..The aim of the present study was to identify and differentiate Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group strains isolated from clinical and environmental sources in different countries...
  24. pmc Fluoroquinolone resistance in Mycobacterium tuberculosis and mutations in gyrA and gyrB
    Andrea von Groll
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    Antimicrob Agents Chemother 53:4498-500. 2009
    ..Forty strains shared the same resistance results for the three fluoroquinolones. However, one strain, with an Asn-533 --> Thr mutation in gyrB, was susceptible to ofloxacin but resistant to moxifloxacin and gatifloxacin...
  25. pmc Primary culture of Mycobacterium ulcerans from human tissue specimens after storage in semisolid transport medium
    Miriam Eddyani
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, B 2000 Antwerpen, Belgium
    J Clin Microbiol 46:69-72. 2008
    ..This transport medium is thus very useful for the confirmation of a diagnosis of Buruli ulcer with specimens collected in the field...
  26. pmc Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
    Andrea von Groll
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    PLoS ONE 5:e10191. 2010
    ..The aim of this study was to evaluate the fitness of drug-susceptible and MDR M. tuberculosis strains of the W-Beijing genotype compared with that of Non-W-Beijing strains...
  27. doi request reprint Fitness study of the RDRio lineage and Latin American-Mediterranean family of Mycobacterium tuberculosis in the city of Rio Grande, Brazil
    Andrea von Groll
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    FEMS Immunol Med Microbiol 58:119-27. 2010
    ..Strains belonging to the LAM family showed a fitness advantage when comparing their rate of growth with that of non-LAM strains, but a significant difference between RD(Rio) and non-RD(Rio) strains was not confirmed...
  28. ncbi request reprint Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis
    A N Umubyeyi
    Mycobacteriology Unit, Institute of Tropical Medecine, Antwerp, Belgium
    Int J Tuberc Lung Dis 10:808-11. 2006
    ..To evaluate the performance of the colorimetric resazurin microtiter assay (REMA) method for the detection of ofloxacin resistance...
  29. ncbi request reprint Rapid drug resistance detection in Mycobacterium tuberculosis: a review of colourimetric methods
    J C Palomino
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    Clin Microbiol Infect 13:754-62. 2007
    ..tuberculosis. Based on published evidence and the high accuracy of colourimetric methods for detecting drug resistance in M. tuberculosis, these methods seem to be appropriate for implementation in high-burden low-resource countries...
  30. ncbi request reprint Diagnosis of drug-resistant tuberculosis: reliability and rapidity of detection
    A Van Deun
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium International Union Against Tuberculosis and Lung Disease, Paris, France
    Int J Tuberc Lung Dis 14:131-40. 2010
    ..No test is fully accurate, and proper pre-selection may allow the use of less accurate but simple screening methods. Conventional slow DST will still be needed for confirmation and for epidemiological monitoring...
  31. ncbi request reprint A microplate indicator-based method for determining the susceptibility of multidrug-resistant Mycobacterium tuberculosis to antimicrobial agents
    Juan Carlos Palomino
    Int J Tuberc Lung Dis 8:1510-1; author reply 1511. 2004
  32. pmc Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples
    Dissou Affolabi
    Laboratoire de Référence des Mycobactéries, 01 BP 817 Cotonou, Benin
    J Clin Microbiol 46:3243-5. 2008
    ..In total 56% of the results were obtained in 10 days by the NRA. NRA performed in liquid medium is rapid and inexpensive and can be easily implemented in low-income countries...
  33. pmc In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings
    Norberto Simboli
    INEI ANLIS Carlos G Malbrán, Av Velez Sarsfield 563, 1281 Buenos Aires, Argentina
    Antimicrob Agents Chemother 49:425-7. 2005
    ..2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory...
  34. ncbi request reprint Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean
    Sylvia Cardoso Leao
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Botucatu, 862 3 degrees andar, São Paulo 04023 062, Brazil
    J Microbiol Methods 61:193-9. 2005
    ..In others, improvement in critical points is still necessary...
  35. ncbi request reprint Comparative evaluation of the nitrate reduction assay, the MTT test, and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis
    Ernesto Montoro
    Laboratorio Nacional de Referencia e Investigaciones en Tuberculosis y Mycobacteria, Instituto de Medicina Tropical Pedro Kouri, Havana, Cuba
    J Antimicrob Chemother 55:500-5. 2005
    ..To evaluate the performance of three rapid low-cost methods for the detection of resistance to first-line drugs in Mycobacterium tuberculosis...
  36. ncbi request reprint [Phenotypic and genotypic methods for detecting multidrug resistance in Mycobacterium tuberculosis]
    Tania Bibiana Porras
    Grupo de Micobacterias, Instituto Nacional de Salud, Bogota, D C, Colombia
    Biomedica 25:22-33. 2005
    ..Expeditious charactization of drug susceptibility in Mycobacterium tuberculosis is difficult and, calls for the design and evaluation of faster, cheaper and more effective new techniques...
  37. pmc Profiling Mycobacterium ulcerans with hsp65
    Sylvia Cardoso Leao
    Emerg Infect Dis 11:1795-6. 2005
  38. ncbi request reprint Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers
    Farida Nateche
    Faculté de Biologie, Université des Sciences et de Technologie, Houari Boumediene, Alger, Algeria
    J Med Microbiol 55:857-60. 2006
    ..3 and 99.2 %, respectively. The REMA plate method appears to be a reliable method for the rapid determination of multidrug-resistant tuberculosis and is a good alternative for use in resource-limited countries such as Algeria...
  39. ncbi request reprint MODS assay for the diagnosis of TB
    Juan Carlos Palomino
    N Engl J Med 356:188; author reply 189. 2007
  40. ncbi request reprint Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low-resource laboratories
    Dihadenys Lemus
    Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, La Lisa, La Habana, Cuba
    J Med Microbiol 55:861-3. 2006
    ..8 %. The NRA was easy to perform and represents a useful tool for rapid and accurate determination of drug-resistant M. tuberculosis strains in low-resource countries...
  41. doi request reprint Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays
    Dissou Affolabi
    Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Benin
    J Med Microbiol 57:1024-7. 2008
    ..A complete agreement was found between NRA and PM, while one false RMP-susceptible result was found by REMA. REMA and NRA tests are rapid and inexpensive, and could be good alternatives to the conventional PM in low-resource countries...
  42. ncbi request reprint Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis
    Dihadenys Lemus
    Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    J Antimicrob Chemother 54:130-3. 2004
    ..To study the performance of three rapid low cost methods for the detection of rifampicin resistance...