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| E HermansSummaryCountry: Belgium Publications
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Publications
Biochemical and pharmacological control of the multiplicity of coupling at G-protein-coupled receptorsEmmanuel Hermans
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, FARL 54 10, Avenue Hippocrate 54, B 1200 Brussels, Belgium
Pharmacol Ther 99:25-44. 2003..This review aims at summarising the different studies that support the concept of multiplicity of G-protein coupling. The physiological and pharmacological relevance of this coupling promiscuity will be discussed...- Complex involvement of pertussis toxin-sensitive G proteins in the regulation of type 1alpha metabotropic glutamate receptor signaling in baby hamster kidney cellsE Hermans
Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, United Kingdom
Mol Pharmacol 58:352-60. 2000..The latter are involved in the negative control of phospholipase C activity while also influencing the rate of desensitization of the mGlu1alpha receptor... 
Structural, signalling and regulatory properties of the group I metabotropic glutamate receptors: prototypic family C G-protein-coupled receptorsE Hermans
Laboratoire de Pharmacologie, Université catholique de Louvain 54 10, B 1200 Brussels, Belgium
Biochem J 359:465-84. 2001....- Reversible and non-competitive antagonist profile of CPCCOEt at the human type 1alpha metabotropic glutamate receptorE Hermans
Department of Cell Physiology and Pharmacology, University of Leicester, UK
Neuropharmacology 37:1645-7. 1998..76+/-0.18; n=3). This suggests that CPCCOEt antagonizes type 1alpha metabotropic glutamate receptor activation by interacting with a site distinct from the agonist binding site... - Inhibition of N-linked glycosylation of the human type 1alpha metabotropic glutamate receptor by tunicamycin: effects on cell-surface receptor expression and functionN Mody
Department of Cell Physiology and Pharmacology, University of Leicester, UK
Neuropharmacology 38:1485-92. 1999..Our data suggest that non-N-glycosylated human mGlu1alpha receptors can traffic to the cell surface and activate phospholipase C... - Distinct regions of C-terminus of the high affinity neurotensin receptor mediate the functional coupling with pertussis toxin sensitive and insensitive G-proteinsMustapha Najimi
Laboratoire de Pharmacologie Exprimentale (FARL, , , Avenue Hippocrate 54, B-1200, Brussels, Belgium
FEBS Lett 512:329-33. 2002..This region was also found to be involved in the control of receptor internalization. However, PTx failed to impair internalization, indicating that these two properties are not directly related... - Multiplicity and regulation of G-protein couplingsE Hermans
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain
Bull Mem Acad R Med Belg 164:255-62. 2009.... - Down-regulation of neurotensin receptors after ligand-induced internalization in rat primary cultured neuronsE Hermans
Laboratory of Pharmacology, Catholic University of Louvain, Brussels, Belgium
Neurochem Int 31:291-9. 1997..These results indicate that the neurotensin-induced receptor internalization in cultured neurons is irreversible and that it is followed by a down-regulation of the receptor through a degradative process... - Pertussis toxin-sensitive modulation of glutamate transport by endothelin-1 type A receptors in glioma cellsMustapha Najimi
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, 54 10, Avenue Hippocrate 54, 1200 Bruxelles, Belgium
Biochim Biophys Acta 1668:195-202. 2005.... - Cytoskeleton-related trafficking of the EAAC1 glutamate transporter after activation of the G(q/11)-coupled neurotensin receptor NTS1Mustapha Najimi
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, Avenue Hippocrate 54, 1200, Brussels, Belgium
FEBS Lett 523:224-8. 2002..Together, these results provide evidence for an acute regulation of EAAC1 trafficking after activation of a G protein-coupled receptor... 
PICK1 expression in reactive astrocytes within the spinal cord of amyotrophic lateral sclerosis (ALS) ratsM C Focant
Group of Neuropharmacology, Institute of Neuroscience, Universite Catholique de Louvain, Brussels, Belgium
Neuropathol Appl Neurobiol 39:231-42. 2013..In this study, we examined the expression of PICK1 in the spinal cord of transgenic rats expressing a mutated form of the human superoxide dismutase 1 (hSOD1(G93A) ) during the progression of the disease...- Enhanced expression of the high affinity glutamate transporter GLT-1 in C6 glioma cells delays tumour progression in ratNicolas Vanhoutte
Laboratory of Experimental Pharmacology, Universite Catholique de Louvain, 54 10, av Hippocrate 54, 1200 Brussels, Belgium
Exp Neurol 218:56-63. 2009.... - Transcriptional regulation of the tyrosine hydroxylase gene by neurotensin in human neuroblastoma CHP212 cellsM Najimi
Laboratoire de Pharmacologie, Universite Catholique de Louvain, Brussels, Belgium
Metab Brain Dis 16:165-74. 2001.... - Analogues and homologues of N-palmitoylethanolamide, a putative endogenous CB(2) cannabinoid, as potential ligands for the cannabinoid receptorsD M Lambert
Unite de Chimie pharmaceutique et de Radiopharmacie, département des sciences pharmaceutiques, Universite Catholique de Louvain, UCL 7340, Avenue Mounier 73, B 1200, Brussels, Belgium
Biochim Biophys Acta 1440:266-74. 1999.... - Differential modulation of AP-1- and CRE-driven transcription by cannabinoid agonists emphasizes functional selectivity at the CB1 receptorB Bosier
Unité de Chimie Pharmaceutique et de Radiopharmacie UCL 7340, Universite Catholique de Louvain, Brussels, Belgium
Br J Pharmacol 155:24-33. 2008..Herein, we characterized their influence on cAMP response element (CRE) and activator protein 1 (AP-1)-mediated regulation of gene transcription... - Glutamate-induced glioma cell proliferation is prevented by functional expression of the glutamate transporter GLT-1Nicolas Vanhoutte
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, 54 10, av Hippocrate 54, 1200 Brussels, Belgium
FEBS Lett 582:1847-52. 2008..This suggests that impaired glutamate uptake capacity in glioma cells indirectly contributes to their proliferation... - Acute up-regulation of glutamate uptake mediated by mGluR5a in reactive astrocytesCéline Vermeiren
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, Brussels, Belgium
J Neurochem 94:405-16. 2005..We conclude that mGluR5a contributes to a dynamic control of GLT-1 function in activated astrocytes, acting as a glial sensor of the extracellular glutamate concentration in order to acutely regulate the excitatory transmission... - Opposite regulation of metabotropic glutamate receptor 3 and metabotropic glutamate receptor 5 by inflammatory stimuli in cultured microglia and astrocytesJ V Berger
Institute of Neuroscience, Group of Neuropharmacology, Universite Catholique de Louvain, Avenue Hippocrate B1 54 10, B 1200 Brussels, Belgium
Neuroscience 205:29-38. 2012..As neuroinflammation commonly occurs in several nervous diseases, its influence on mGluR expression should be taken into account when considering these receptors as future drug targets... - Increased dopamine uptake in striatal synaptosomes after treatment of rats with amantadineG Page
Laboratoire de Pharmacologie, Université catholique de Louvain FARL 54 10, Avenue Hippocrate 54, B 1200, Brussels, Belgium
Eur J Pharmacol 403:75-80. 2000..An indirect mechanism would involve presynaptic dopamine receptors that control the activity of the DAT in response to the increased dopamine concentration in the synaptic cleft... - Amantadine and memantine induce the expression of the glial cell line-derived neurotrophic factor in C6 glioma cellsAnne-Sophie Caumont
, , Avenue Hippocrate 54.10, 1200 Bruxelles, Belgium
Neurosci Lett 394:196-201. 2006..Together, these results demonstrate that the neuroprotective effect of amantadine and memantine could involve the regulation of GDNF production by glial cells... - Concomitant activation of adenylyl cyclase suppresses the opposite influences of CB(1) cannabinoid receptor agonists on tyrosine hydroxylase expressionBarbara Bosier
Unite de Chimie pharmaceutique et de Radiopharmacie, Universite Catholique de Louvain, Brussels, Belgium
Biochem Pharmacol 77:216-27. 2009..Together our results suggest that the physiological modulation of TH expression by cannabinoid agonists in dopaminergic neurons would be influenced by additional endogenous inputs... - Cultured astrocytes derived from corpus callosum or cortical grey matter show distinct glutamate handling propertiesStephanie Goursaud
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, Brussels, Belgium
J Neurochem 108:1442-52. 2009..Moreover, the expression and activity of the astroglial components involved in the control of glutamatergic transmission are reinforced when these cells are maintained under conditions mimicking a gliotic environment... - Activation of VIP/PACAP type 2 receptor by the peptide histidine isoleucine in astrocytes influences GLAST-mediated glutamate uptakeStephanie Goursaud
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, Av Hippocrate 54 10, 1200 Brussels, Belgium
J Neurochem 105:1165-75. 2008..24 +/- 8.74 and 43.00 +/- 6.36%, when considering the 72 and 55 kDa immunoreactive proteins, respectively). Such cross-talk between PHI and glutamate transmission systems in glial cells opens attractive perspectives in neuropharmacology... - Agonist selective modulation of tyrosine hydroxylase expression by cannabinoid ligands in a murine neuroblastoma cell lineBarbara Bosier
Unité de Chimie Pharmaceutique et de Radiopharmacie UCL 7340, Universite Catholique de Louvain, Brussels, Belgium
J Neurochem 102:1996-2007. 2007.... - Loss of metabotropic glutamate receptor-mediated regulation of glutamate transport in chemically activated astrocytes in a rat model of amyotrophic lateral sclerosisCéline Vermeiren
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, Brussels, Belgium
J Neurochem 96:719-31. 2006..The failure of activated mGluR5 to increase glutamate uptake in astrocytes derived from this animal model of ALS supports the theory of glutamate excitotoxicity in the pathogenesis of the disease... - Induction of glial glutamate transporters in adult mesenchymal stem cellsIsabelle de Hemptinne
, , Brussels, Belgium
J Neurochem 91:155-66. 2004..Although the astrocytic nature of these cells remains to be confirmed, this observation gives support to the study of mesenchymal stem cells as a promising tool for the treatment of neurological diseases involving glutamate excitoxicity... - Versatility of GPCR recognition by drugs: from biological implications to therapeutic relevanceBarbara Bosier
Unité de Chimie Pharmaceutique et de Radiopharmacie UCL 7340, Universite Catholique de Louvain, B 1200 Brussels, Belgium
Trends Pharmacol Sci 28:438-46. 2007..Although such complexity should be regarded as an opportunity to gain pharmacological specificity, unraveling the physiological implications of these concepts is essential before their therapeutic relevance can be defined... - Involvement of the sigma 1 receptor in the modulation of dopaminergic transmission by amantadineMagali Peeters
Laboratoire de Pharmacologie Expérimentale FARL, Universite Catholique de Louvain, 54 10, Avenue Hippocrate 54, B 1200 Brussels, Belgium
Eur J Neurosci 19:2212-20. 2004..Together, these data demonstrate that aminoadamantanes behave as sigma1 receptor agonists, and confirm an involvement of this receptor in modulating dopamine receptors exerted by therapeutically relevant concentrations of amantadine... - Selective up-regulation of GLT-1 in cultured astrocytes exposed to soluble mediators released by activated microgliaSébastien Tilleux
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, Brussels, Belgium
Neurochem Int 55:35-40. 2009..Taken together, these results suggest that the GLT-1-dependent control of glutamate neurotransmission by either naive or chemically activated astrocytes is influenced by microglia-mediated inflammation... - Distinct expression and regulation of the glutamate transporter isoforms GLT-1a and GLT-1b in cultured astrocytes from a rat model of amyotrophic lateral sclerosis (hSOD1G93A)Stephanie Goursaud
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, Brussels, Belgium
Neurochem Int 55:28-34. 2009.... - In vitro differentiated neural stem cells express functional glial glutamate transportersNicolas Vanhoutte
, , Avenue Hippocrate 54, B-1200 Brussels, Belgium
Neurosci Lett 370:230-5. 2004..Together, these results indicate that in vitro differentiation of cultured neural stem cells can give rise to distinct populations of astrocytes expressing functional glutamate transporters... - Molecular and functional characterisation of glutamate transporters in rat cortical astrocytes exposed to a defined combination of growth factors during in vitro differentiationCéline Vermeiren
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, av Hippocrate 54, 1200 Brussels, Belgium
Neurochem Int 46:137-47. 2005.... - Reciprocal influences of CB1 cannabinoid receptor agonists on ERK and JNK signalling in N1E-115 cellsBarbara Bosier
Unité de Chimie Pharmaceutique et de Radiopharmacie UCL 7340, Universite Catholique de Louvain, 73 40, Av E Mounier, B 1200 Brussels, Belgium
FEBS Lett 582:3861-7. 2008..These data indicate that complex interactions of the CB1 receptor with intracellular signalling partners controlling MAPK activities may explain the apparent disparities in cellular responses to functional selective agonists... - In vitro evidence for impaired neuroprotective capacities of adult mesenchymal stem cells derived from a rat model of familial amyotrophic lateral sclerosis (hSOD1(G93A))Cédric Boucherie
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, 54 10, av Hippocrate 54, 1200 Brussels, Belgium
Exp Neurol 212:557-61. 2008.... - Specific regulation of rat glial cell line-derived neurotrophic factor gene expression by riluzole in C6 glioma cellsAnne Sophie Caumont
Laboratoire de Pharmacologie Expérimentale, Universite Catholique de Louvain, 54 10, Avenue Hippocrate 54, 1200 Bruxelles, Belgium
J Neurochem 97:128-39. 2006..This study suggests that the regulation of GDNF gene transcription in glial cells could contribute to the pharmacological properties of riluzole and possibly other neuroprotective drugs... - Neuroinflammation and regulation of glial glutamate uptake in neurological disordersSébastien Tilleux
Laboratoire de Pharmacologie Expérimentale, Faculte de Medecine, Universite Catholique de Louvain, Brussels, Belgium
J Neurosci Res 85:2059-70. 2007..The aim of this review is to summarize and discuss the data showing an influence of inflammatory mediators and related free radicals on the expression and activity of glial glutamate transporters... - Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivityCasim A Sarkar
Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Proc Natl Acad Sci U S A 105:14808-13. 2008..These approaches may alleviate existing bottlenecks in structural studies of these targets by providing sufficient quantities of stable variants in defined conformational states... - Generation of model cell lines expressing recombinant G-protein-coupled receptorsEmmanuel Hermans
Laboratory of Experimental Pharmacology, Catholic University of Louvain, Brussels, Belgium
Methods Mol Biol 259:137-53. 2004..This chapter gives a brief overview of the different techniques and provides methodology for the generation of transiently transfected cells and for selection, isolation and maintenance of stable transfected cell lines... - Differential involvement of intracellular domains of the rat NTS1 neurotensin receptor in coupling to G proteins: a molecular basis for agonist-directed trafficking of receptor stimulusDelphine Skrzydelski
, , , Paris, France
Mol Pharmacol 64:421-9. 2003..They also suggest that the differential involvement of these domains in coupling to G proteins might represent a molecular basis for agonist-selective responses through G protein-coupled receptors...