E Goormaghtigh

Summary

Country: Belgium

Publications

  1. ncbi request reprint Reconstitution of holin activity with a synthetic peptide containing the 1-32 sequence region of EJh, the EJ-1 phage holin
    Amparo Haro
    Insto de Quimica Fisica Rocasolano, Consejo Superior de Investigaciones Cientificas, Madrid 28006, Spain
    J Biol Chem 278:3929-36. 2003
  2. ncbi request reprint Fourier transform infrared (FTIR) spectroscopy to monitor the cellular impact of newly synthesized platinum derivatives
    Gilles Berger
    Laboratoire de Chimie Pharmaceutique Organique, Institut de Pharmacie, Faculty of Sciences, Universite Libre de Bruxelles ULB, Brussels, Belgium
    Int J Oncol 37:679-86. 2010
  3. doi request reprint Infrared imaging in breast cancer: automated tissue component recognition and spectral characterization of breast cancer cells as well as the tumor microenvironment
    Audrey Bénard
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles ULB, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Analyst 139:1044-56. 2014
  4. doi request reprint FTIR spectral signature of anticancer drug effects on PC-3 cancer cells: is there any influence of the cell cycle?
    Allison Derenne
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Universite Libre de Bruxelles, Campus Plaine CP206 02, Bld du Triomphe 2, CP206 2, B1050 Brussels
    Analyst 138:3998-4005. 2013
  5. doi request reprint A new dimension for cell identification by FTIR spectroscopy: depth profiling in attenuated total reflection
    A Gaigneaux
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, CP 206 2, Universite Libre de Bruxelles ULB, Bld du Triomphe, Acces 2, B 1050 Brussels, Belgium
    Analyst 138:4070-5. 2013
  6. doi request reprint The effect of anticancer drugs on seven cell lines monitored by FTIR spectroscopy
    Allison Derenne
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206 02, Universite Libre de Bruxelles, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Analyst 137:3255-64. 2012
  7. doi request reprint Protein secondary structure content in solution, films and tissues: redundancy and complementarity of the information content in circular dichroism, transmission and ATR FTIR spectra
    Erik Goormaghtigh
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206 02 Université Libre de Bruxelles, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Biochim Biophys Acta 1794:1332-43. 2009
  8. pmc Evaluation of the information content in infrared spectra for protein secondary structure determination
    Erik Goormaghtigh
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Universite Libre de Bruxelles, Brussels, Belgium
    Biophys J 90:2946-57. 2006
  9. ncbi request reprint Lipid membrane binding of NK-lysin
    J M Ruysschaert
    Laboratoire de Chimie Physique des Macromolecules aux Interfaces, Universite Libre de Bruxelles, Brussels, Belgium
    FEBS Lett 425:341-4. 1998
  10. ncbi request reprint Detection of structural and functional asymmetries in P-glycoprotein by combining mutagenesis and H/D exchange measurements
    C Vigano
    Centre de Biologie Structurale et de Bioinformatique CBSB, Universite Libre de Bruxelles, CP 206 2, Bd du Triomphe, 1050 Brussels, Belgium
    Chem Phys Lipids 122:121-35. 2003

Collaborators

Detail Information

Publications48

  1. ncbi request reprint Reconstitution of holin activity with a synthetic peptide containing the 1-32 sequence region of EJh, the EJ-1 phage holin
    Amparo Haro
    Insto de Quimica Fisica Rocasolano, Consejo Superior de Investigaciones Cientificas, Madrid 28006, Spain
    J Biol Chem 278:3929-36. 2003
    ..When analyzed by atomic force microscopy, peptide-induced membrane lesions are visualized as transbilayer holes. These findings are the first evidence for a lytic domain in holins and for the nature of membrane lesions caused by them...
  2. ncbi request reprint Fourier transform infrared (FTIR) spectroscopy to monitor the cellular impact of newly synthesized platinum derivatives
    Gilles Berger
    Laboratoire de Chimie Pharmaceutique Organique, Institut de Pharmacie, Faculty of Sciences, Universite Libre de Bruxelles ULB, Brussels, Belgium
    Int J Oncol 37:679-86. 2010
    ..The original pharmacological approach proposed here could, therefore, avoid large-scale pharmacological experiments to find hits within a given chemical series...
  3. doi request reprint Infrared imaging in breast cancer: automated tissue component recognition and spectral characterization of breast cancer cells as well as the tumor microenvironment
    Audrey Bénard
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles ULB, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Analyst 139:1044-56. 2014
    ..Finally, we demonstrate that the biochemical information reflected in the epithelial spectra might be clinically relevant for the grading purpose, suggesting potential to improve breast cancer management in the future. ..
  4. doi request reprint FTIR spectral signature of anticancer drug effects on PC-3 cancer cells: is there any influence of the cell cycle?
    Allison Derenne
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Universite Libre de Bruxelles, Campus Plaine CP206 02, Bld du Triomphe 2, CP206 2, B1050 Brussels
    Analyst 138:3998-4005. 2013
    ..Results demonstrate that the FTIR cell cycle signature is very small with respect to the changes induced by paclitaxel...
  5. doi request reprint A new dimension for cell identification by FTIR spectroscopy: depth profiling in attenuated total reflection
    A Gaigneaux
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, CP 206 2, Universite Libre de Bruxelles ULB, Bld du Triomphe, Acces 2, B 1050 Brussels, Belgium
    Analyst 138:4070-5. 2013
    ..It is suggested that the depth profile of the difference spectra could allow a more precise localization of the biochemical modifications arising within the multidrug resistance phenotype...
  6. doi request reprint The effect of anticancer drugs on seven cell lines monitored by FTIR spectroscopy
    Allison Derenne
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206 02, Universite Libre de Bruxelles, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Analyst 137:3255-64. 2012
    ..High throughput systems with 96-well plates are now available and a well established bioassay could be developed in order to provide an objective classifier for potential anticancer drugs...
  7. doi request reprint Protein secondary structure content in solution, films and tissues: redundancy and complementarity of the information content in circular dichroism, transmission and ATR FTIR spectra
    Erik Goormaghtigh
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206 02 Université Libre de Bruxelles, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Biochim Biophys Acta 1794:1332-43. 2009
    ..These new techniques open the way to a new area of research, both in protein conformational response to ligand and imaging at sub-cellular scales...
  8. pmc Evaluation of the information content in infrared spectra for protein secondary structure determination
    Erik Goormaghtigh
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Universite Libre de Bruxelles, Brussels, Belgium
    Biophys J 90:2946-57. 2006
    ..Using the 50-protein database built beforehand to contain as little fold redundancy as possible, the standard error of prediction in cross-validation is 5.5% for the alpha-helix, 6.6% for the beta-sheet, and 3.4% for the beta-turn...
  9. ncbi request reprint Lipid membrane binding of NK-lysin
    J M Ruysschaert
    Laboratoire de Chimie Physique des Macromolecules aux Interfaces, Universite Libre de Bruxelles, Brussels, Belgium
    FEBS Lett 425:341-4. 1998
    ..The molecular mechanism of the membrane-destabilizing effect of NK-lysin is discussed...
  10. ncbi request reprint Detection of structural and functional asymmetries in P-glycoprotein by combining mutagenesis and H/D exchange measurements
    C Vigano
    Centre de Biologie Structurale et de Bioinformatique CBSB, Universite Libre de Bruxelles, CP 206 2, Bd du Triomphe, 1050 Brussels, Belgium
    Chem Phys Lipids 122:121-35. 2003
    ..In particular, results obtained about the structure and function of the nucleotide binding domains (NBD) of P-glycoprotein (Pgp), a multidrug transporter involved in cancer cells resistance to chemotherapy, are described...
  11. ncbi request reprint Analysis of 1H/2H exchange kinetics using model infrared spectra
    Vincent Raussens
    Laboratory for the Structure and Function of Biological Membranes, Structural Biology and Bioinformatics Center, Free University of Brussels, CP 206 2, Boulevard du Triomphe, B 1050 Brussels, Belgium
    Appl Spectrosc 58:68-82. 2004
    ..It is demonstrated that some strategies allow the extraction of both the time dependence and the spectral shape of the underlying contributions...
  12. ncbi request reprint The optimization of protein secondary structure determination with infrared and circular dichroism spectra
    Keith A Oberg
    Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Free University of Brussels ULB, Belgium
    Eur J Biochem 271:2937-48. 2004
    ..Interestingly, it was also found in the course of this study that the amide II band has high information content and could be used alone for secondary structure prediction in place of amide I...
  13. ncbi request reprint A new experimental approach to detect long-range conformational changes transmitted between the membrane and cytosolic domains of LmrA, a bacterial multidrug transporter
    Catherine Vigano
    Service de Structure et Fonction des Membranes Biologiques SFMB, Universite Libre de Bruxelles, P O Box 206 2, Bd du Triomphe, B1050 Brussels, Belgium
    FEBS Lett 530:197-203. 2002
    ..On the other hand, drug binding to the transmembrane helices affects both the structure of the cytosolic regions and the ATPase activity of the nucleotide binding domain...
  14. doi request reprint Breast cancer and melanoma cell line identification by FTIR imaging after formalin-fixation and paraffin-embedding
    M Verdonck
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles, Campus Plaine, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Analyst 138:4083-91. 2013
    ..This last result paves the way towards identification and characterization of cellular subtypes on FFPE tissue sections by FTIR imaging, indicating that this analysis technique could become a potential useful tool in cancer research...
  15. ncbi request reprint Cell discrimination by attenuated total reflection-Fourier transform infrared spectroscopy: the impact of preprocessing of spectra
    A Gaigneaux
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, CP 206 2, Free University of Brussels ULB, Bld du Triomphe, Acces 2, B 1050 Brussels, Belgium
    Appl Spectrosc 60:1022-8. 2006
    ..On the other hand, results obtained with derivatives were less sensitive to scaling, a useful feature when scaling is problematic...
  16. doi request reprint Cholesterol modulates the exposure and orientation of pulmonary surfactant protein SP-C in model surfactant membranes
    L Gómez-Gil
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles, Campus Plaine CP 206 2, Blvd du Triomphe, B 1050 Brussels, Belgium
    Biochim Biophys Acta 1788:1907-15. 2009
    ....
  17. ncbi request reprint Phosphorylation-induced conformational changes of cystic fibrosis transmembrane conductance regulator monitored by attenuated total reflection-Fourier transform IR spectroscopy and fluorescence spectroscopy
    Vinciane Grimard
    Center for Structural Biology and Bioinformatics, Free University of Brussels, Campus Plaine CP206 2, 1050 Brussels, Belgium
    J Biol Chem 279:5528-36. 2004
    ..Such structural changes may provide better access for the solutes to the nucleotide binding domains and the ion binding site...
  18. doi request reprint Change in the microenvironment of breast cancer studied by FTIR imaging
    S Kumar
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles, Campus Plaine CP 206 2 Bâtiment BC, Blvd du Triomphe, B 1050 Bruxelles, Belgium
    Analyst 138:4058-65. 2013
    ..This chemical imaging approach to analyze the breast cancer microenvironment could be used in the future for improving diagnostics of breast cancer...
  19. ncbi request reprint Conformational changes of the 120-kDa Na+/Ca2+ exchanger protein upon ligand binding: a Fourier transform infrared spectroscopy study
    R I Saba
    , , , 808 Route de Lennik, B-1070, Brussels, Belgium
    Biochemistry 40:3324-32. 2001
    ....
  20. doi request reprint Ligand-receptor interactions in complex media: a new type of biosensors for the detection of coagulation factor VIII
    A Goldzstein
    Laboratoire de Structure et Fonction des Membranes Biologiques, Centre de Biologie Structurale et Bioinformatique, Universite Libre de Bruxelles, Campus Plaine CP206 2, B 1050 Bruxelles, Belgium
    Biosens Bioelectron 24:1831-6. 2009
    ..A linear response of the sensors as a function of the factor VIII concentration is described for different lipid membrane compositions...
  21. doi request reprint IR spectroscopy as a new tool for evidencing antitumor drug signatures
    Régis Gasper
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles ULB, Brussels, Belgium
    Biochim Biophys Acta 1788:1263-70. 2009
    ..The most spectacular data obtained strongly suggest a modification in the nature of the cell lipids...
  22. doi request reprint FTIR spectral signature of the effect of cardiotonic steroids with antitumoral properties on a prostate cancer cell line
    Régis Gasper
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles ULB, Belgium
    Biochim Biophys Acta 1802:1087-94. 2010
    ....
  23. ncbi request reprint Structural modifications in the membrane-bound regions of the gastric H+/K+-ATPase upon ligand binding
    N Baeyens
    Structure and Function of Biological Membranes, Free University of Brussels, Boulevard du Triomphe, B1050 Brussels, Belgium
    Eur J Biochem 268:5135-41. 2001
    ..In the M7-M8 region, the same peptide encompassing the M7 and M8 transmembrane segments was produced for E1 and E2 conformations...
  24. ncbi request reprint The infrared spectrum of human glioma cells is related to their in vitro and in vivo behavior
    A Gaigneaux
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Free University of Brussels ULB, B 1050 Brussels, Belgium
    Exp Cell Res 297:294-301. 2004
    ..We concluded that infrared spectroscopy could usefully complement information provided by more conventional diagnostic and prognostic (e.g., morphological and molecular) approaches...
  25. ncbi request reprint Hydrogen-deuterium exchange in membrane proteins monitored by IR spectroscopy: a new tool to resolve protein structure and dynamics
    C Vigano
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Free University of Brussels, CP 206 2, Boulevard du Triomphe, B 1050 Brussels, Belgium
    Biopolymers 74:19-26. 2004
    ..The study of one membrane protein, the gastric H(+),K(+)-ATPase, demonstrates the usefulness of (1)H/(2)H exchange kinetics for the understanding of the molecular movement related to the catalytic activity...
  26. pmc Evaluation of the ordering of membranes in multilayer stacks built on an ATR-FTIR germanium crystal with atomic force microscopy: the case of the H(+),K(+)-ATPase-containing gastric tubulovesicle membranes
    Dimitri Ivanov
    Laboratory for Polymer Physics, Laboratory for the Structure and Function of Biological Membranes, Structural Biology and Bioinformatics Center, Free University of Brussels, Brussels, Belgium
    Biophys J 87:1307-15. 2004
    ..9. It could therefore be concluded that the membrane tilt does not significantly contribute to the infrared dichroism, even for the largest thicknesses tested...
  27. ncbi request reprint Conformational changes of the 120-kDa Na+/Ca2+ exchanger protein upon ligand binding: a Fourier transform infrared spectroscopy study
    Rami I Saba
    Laboratoire de Pharmacodynamie, Facultés de Medécine et des Sciences, Brussels, Belgium
    Ann N Y Acad Sci 976:97-9. 2002
  28. doi request reprint Antiparallel beta-sheet: a signature structure of the oligomeric amyloid beta-peptide
    Emilie Cerf
    Center for Structural Biology and Bioinformatics, Laboratory for Structure and Function of Biological Membranes, Faculte des Sciences, Universite Libre de Bruxelles, CP 206 2, Blvd du Triomphe, B 1050 Brussels, Belgium
    Biochem J 421:415-23. 2009
    ..We discuss our results in terms of a possible organization of the antiparallel beta-sheets in Abeta oligomers, which may be related to reported effects of these highly toxic species in the amyloid pathogenesis associated with AD...
  29. pmc Conformational changes in gastric H+/K+-ATPase monitored by difference Fourier-transform infrared spectroscopy and hydrogen/deuterium exchange
    Frantz Scheirlinckx
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Free University of Brussels, CP206 2, Boulevard du Triomphe, B 1050 Brussels, Belgium
    Biochem J 382:121-9. 2004
    ..The present study suggests that conformational changes in the catalytic cycle imply secondary-structure rearrangements of small hinge regions that have an impact on large domain re-organizations...
  30. ncbi request reprint Attenuated total reflection IR spectroscopy as a tool to investigate the orientation and tertiary structure changes in fusion proteins
    I Martin
    Structure and Function of Biological Membranes, Center of Structural Biology and Bioinformatics, Universite Libre de Bruxelles, Boulevard du Triomphe C P 206 2, B 1050, Brussels, Belgium
    Biochim Biophys Acta 1614:97-103. 2003
    ..Recent developments suggest that the method could also be used to detect changes located in the membrane domains and to identify intermediate structural states involved in the fusion process...
  31. doi request reprint The FTIR spectrum of prostate cancer cells allows the classification of anticancer drugs according to their mode of action
    Allison Derenne
    Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Universite Libre de Bruxelles ULB, Bld du Triomphe 2, CP206 2, B1050 Brussels, Belgium
    Analyst 136:1134-41. 2011
    ..Taken all together these data suggest that FTIR could be used for the classification of drug action...
  32. pmc Characterization of the interactions between fluoroquinolone antibiotics and lipids: a multitechnique approach
    Hayet Bensikaddour
    Universite Catholique de Louvain, Faculty of Medicine, Unité de Pharmacologie Cellulaire et Moléculaire, Brussels, Belgium
    Biophys J 94:3035-46. 2008
    ..All together, differences of ciprofloxacin as compared to moxifloxacin in their interactions with lipids could explain differences in their cellular accumulation and susceptibility to efflux transporters...
  33. ncbi request reprint Interactions involved in the realignment of membrane-associated helices. An investigation using oriented solid-state NMR and attenuated total reflection Fourier transform infrared spectroscopies
    Christopher Aisenbrey
    Institut Faculté de Chimie, Université Louis Pasteur CNRS LC3 UMR7177, 4 rue Blaise Pascal, Strasbourg 67070, France
    J Biol Chem 281:7708-16. 2006
    ..Notably, the resulting scale for whole residue transitions from the bilayer interface to the hydrophobic membrane interior is obtained from extended helical sequences in lipid bilayers...
  34. ncbi request reprint Chemometric tools for classification and elucidation of protein secondary structure from infrared and circular dichroism spectroscopic measurements
    Susana Navea
    Chemometrics Group, Department of Analytical Chemistry, Universitat de Barcelona, Barcelona, Spain
    Proteins 63:527-41. 2006
    ..The predictive ability of the models built with the selected spectral regions has a quality similar to previous classical approaches...
  35. pmc Rationally selected basis proteins: a new approach to selecting proteins for spectroscopic secondary structure analysis
    Keith A Oberg
    Structural Biology and Bioinformatics Center, Structure and Function of Biological Membranes Laboratory, Free University of Brussels ULB, B 1050 Brussels, Belgium
    Protein Sci 12:2015-31. 2003
    ....
  36. ncbi request reprint Insight into the factors influencing the backbone dynamics of three homologous proteins, dendrotoxins I and K, and BPTI: FTIR and time-resolved fluorescence investigations
    Michelle Hollecker
    Centre de Biophysique Moleculaire, UPR 4301 CNRS, affiliée Université Orléans et INSERM, rue Charles Sadron, 45071 Orleans Cedex 2, France
    Biochemistry 41:15267-76. 2002
    ..The structural factors suspected to be responsible for the differences in internal flexibility of the two toxins could play a significant role in determining their functional properties...
  37. ncbi request reprint Translocation of amino acyl residues from the membrane interface to the hydrophobic core: thermodynamic model and experimental analysis using ATR-FTIR spectroscopy
    Christopher Aisenbrey
    Universite Louis Pasteur, Institut Faculté de Chimie, Strasbourg, France
    Mol Membr Biol 23:363-74. 2006
    ....
  38. ncbi request reprint Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids
    Juha Matti Alakoskela
    Helsinki Biophysics and Biomembrane Group, Institute of Biomedicine Biochemistry, P O Box 63, University of Helsinki, 00014 Helsinki, Finland
    Biochemistry 45:13447-53. 2006
    ..The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing...
  39. ncbi request reprint Modulation of the membrane orientation and secondary structure of the C-terminal domains of Bak and Bcl-2 by lipids
    Alejandro Torrecillas
    Departamento de Bioquimica y Biologia Molecular A, Facultad de Veterinaria, Universidad de Murcia, Apartado 4021, E 30080 Murcia, Spain
    Biochemistry 44:10796-809. 2005
    ....
  40. ncbi request reprint Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells
    Anthoula Gaigneaux
    Laboratory of Structure and Function of Biological Membranes, Free University of Brussels, Belgium
    Eur J Biochem 269:1968-73. 2002
    ..Lipid and nucleic acid decreased, relatively, while the protein content increased...
  41. ncbi request reprint The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton
    Michèle Moes
    Laboratoire de Biologie et Physiologie Intégrée CNRS GDRE ITI, University of Luxembourg, L 1511 Luxembourg, Grand Duchy of Luxembourg, Luxembourg
    J Biol Chem 282:17280-8. 2007
    ..Our data provide the first direct evidence that IBS2 in the talin rod is essential to link integrins to the cytoskeleton...
  42. pmc Infrared spectroscopy study on the conformational changes leading to pore formation of the toxin sticholysin II
    Jorge Alegre-Cebollada
    Departamento de Bioquimica y Biologia Molecular I, Facultad de Ciencias Quimicas, Universidad Complutense, 28040 Madrid, Spain
    Biophys J 93:3191-201. 2007
    ..1)H/(2)H exchange experiments sustain a mainly superficial interaction between StnII and the membrane, with no protection of the beta-sandwich. The implications of the results in the mechanism of pore formation are discussed...
  43. ncbi request reprint Protein concentration is not an absolute prerequisite for the determination of secondary structure from circular dichroism spectra: a new scaling method
    Vincent Raussens
    Laboratory for Structure and Function of Biological Membranes, Structural Biology and Bioinformatics Center, Free University of Brussels, Campus Plaine, CP 206 2, Boulevard du Triomphe, Brussels B 1050, Belgium
    Anal Biochem 319:114-21. 2003
    ....
  44. ncbi request reprint Detection of apolipoprotein B100 early conformational changes during oxidation
    Anita Krisko
    Mediterranean Institute for Life Science, Mestrovicevo setaliste bb, HR 21000, Split, Croatia
    Biochim Biophys Acta 1768:2923-30. 2007
    ..These observations provide new insights into the modifications of LDL particles upon oxidation...
  45. ncbi request reprint The papaya Kunitz-type trypsin inhibitor is a highly stable beta-sheet glycoprotein
    Mohamed Azarkan
    University of Brussels, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme CP 609, 808, Route de Lennik, Bz 1070 Brussels, Belgium
    Biochim Biophys Acta 1764:1063-72. 2006
    ..Instead, the soybean inhibitor was completely degraded after 2 h incubation with this aspartic protease. All these data demonstrated the high stability of the papaya trypsin inhibitor...
  46. pmc Energetics and partition of two cecropin-melittin hybrid peptides to model membranes of different composition
    Margarida Bastos
    CIQ UP Department of Chemistry, Faculty of Sciences, University of Porto, P 4169 007 Porto, Portugal
    Biophys J 94:2128-41. 2008
    ..The importance of negatively charged lipids on the discrimination between bacterial and eukaryotic membranes is emphasized...
  47. ncbi request reprint Filipin orientation revealed by linear dichroism. Implication for a model of action
    Silvia C D N Lopes
    Centro de Quimica Fisica Molecular, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    J Am Chem Soc 126:5396-402. 2004
    ..These findings were combined with spectroscopy and microscopy data in the literature, solving controversial matters of debate...
  48. ncbi request reprint Evidence of an intramolecular interaction between the two domains of the BlaR1 penicillin receptor during the signal transduction
    Sophie Hanique
    Centre d Ingénierie des Protéines, Institut de Chimie, Universite de Liege, B 4000 Sart Tilman, Belgium
    J Biol Chem 279:14264-72. 2004
    ..licheniformis BlaR-CTD...