Research Topics
Genomes and GenesSpecies | Chris VerhofstedeSummaryAffiliation: Ghent University Country: Belgium Publications
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Detail Information
Publications
Transmitted drug resistance, selection of resistance mutations and moderate antiretroviral efficacy in HIV-2: analysis of the HIV-2 Belgium and Luxembourg databaseJean Ruelle
Universite Catholique de Louvain, AIDS Reference Laboratory, Avenue Hippocrate 54 5492, 1200 Bruxelles, Belgium
BMC Infect Dis 8:21. 2008..Guidelines established for the treatment of HIV-1 infection and genotype interpretation do not apply for HIV-2. Data about antiretroviral (ARV) drug efficacy and resistance mutations is scarce...- HIV-1 V3 envelope deep sequencing for clinical plasma specimens failing in phenotypic tropism assaysIna Vandenbroucke
Tibotec Virco Virology BVBA, Mechelen, Belgium
AIDS Res Ther 7:4. 2010..While good concordance was observed between gp120 phenotyping and prediction of R5-tropic virus, the results suggest that accurate prediction of X4-tropic virus would require further algorithm development... 
Concordance between HIV-1 genotypic coreceptor tropism predictions based on plasma RNA and proviral DNAC Verhofstede
AIDS Reference Laboratory and AIDS Reference Centre, Ghent University and Ghent University Hospital, Ghent, Belgium
HIV Med 12:544-52. 2011..The aim of the study was to evaluate the use of proviral DNA as a source of viral genetic material for genotypic coreceptor tropism testing (GTT)...- Viral load assay sensitivity and low level viremia in HAART treated HIV patientsChris Verhofstede
Ghent University, AIDS Reference Laboratory, De Pintelaan 185, B 9000 Ghent, Belgium
J Clin Virol 47:335-9. 2010..The significance of a viral load between 20 and 50 copies/mL remains unclear... 
Drug-resistant variants that evolve during nonsuppressive therapy persist in HIV-1-infected peripheral blood mononuclear cells after long-term highly active antiretroviral therapyChris Verhofstede
AIDS Reference Laboratory, Ghent University Hospital, Belgium
J Acquir Immune Defic Syndr 35:473-83. 2004..These data clearly show that virus variants that are able to replicate for a certain period enter the latent reservoir and remain archived in the PBMCs for a very long period...- Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, KenyaKim Steegen
AIDS Reference Laboratory, Ghent University, Ghent, Belgium
AIDS Res Ther 6:12. 2009.... - CXCR4-using HIV type 1 variants are more commonly found in peripheral blood mononuclear cell DNA than in plasma RNAChris Verhofstede
AIDS Reference Laboratory, Ghent University, Ghent, Belgium
J Acquir Immune Defic Syndr 50:126-36. 2009..To compare the distribution of R5-like and X4-like HIV-1 envelope sequences in plasma and peripheral blood mononuclear cell (PBMC)... - Detection of drug resistance mutations as a predictor of subsequent virological failure in patients with HIV-1 viral rebounds of less than 1,000 RNA copies/mlChris Verhofstede
AIDS Reference Laboratory, Ghent University Hospital, Belgium
J Med Virol 79:1254-60. 2007..Most importantly, detection of resistance mutations in viral rebound samples was predictive of subsequent virological failure... - A sensitive in-house RT-PCR genotyping system for combined detection of plasma HIV-1 and assessment of drug resistanceKim Steegen
International Centre for Reproductive Health, University Hospital, Ghent, Belgium
J Virol Methods 133:137-45. 2006..The assay enables the accurate follow-up of patients under treatment at a significantly reduced cost compared to the currently available commercial assays for viral load assessment and genotyping... - Impact of Delta 32-CCR5 heterozygosity on HIV-1 genetic evolution and variability--a study of 4 individuals infected with closely related HIV-1 strainsKristen Chalmet
AIDS Reference Laboratory, Ghent University and Ghent University Hospital, De Pintelaan, 185 Block A, B 9000 Gent, Belgium
Virology 379:213-22. 2008.... - Feasibility of detecting human immunodeficiency virus type 1 drug resistance in DNA extracted from whole blood or dried blood spotsKim Steegen
International Centre for Reproductive Health, Ghent University, Belgium
J Clin Microbiol 45:3342-51. 2007..The use of DNA extracted from whole blood or DBS for the detection of archived drug resistance mutations deserves further study... - Diversity of the human immunodeficiency virus type 1 (HIV-1) env sequence after vertical transmission in mother-child pairs infected with HIV-1 subtype AChris Verhofstede
AIDS Reference Laboratory, Ghent University Hospital, Ghent, Belgium
J Virol 77:3050-7. 2003..Phylogenetic tree topologies argue against selection processes as the major mechanism driving mother-to-child transmission but support the hypothesis that virus variability is mainly driven by the inoculum level and/or exposure time... - Evaluation of two commercially available alternatives for HIV-1 viral load testing in resource-limited settingsKim Steegen
International Centre for Reproductive Health, University Hospital, Ghent, Belgium
J Virol Methods 146:178-87. 2007..Hence, false positive results and the need for an expensive PCR instrument for the Generic HIV viral load assays still limit the implementation of these tests in less equipped, less experienced laboratories... - Epidemiological study of phylogenetic transmission clusters in a local HIV-1 epidemic reveals distinct differences between subtype B and non-B infectionsKristen Chalmet
Ghent University, Belgium
BMC Infect Dis 10:262. 2010.... - Presence of CXCR4-using HIV-1 in patients with recently diagnosed infection: correlates and evidence for transmissionKristen Chalmet
AIDS Reference Laboratory, Ghent University, Belgium
J Infect Dis 205:174-84. 2012..The prevalence and correlates of CXCR4-use in recently diagnosed patients and the impact of X4/DM transmission remain largely unknown... - Placental malaria and perinatal transmission of human immunodeficiency virus type 1Ingrid Inion
International Centre for Reproductive Health, Ghent University, Ghent, Belgium
J Infect Dis 188:1675-8. 2003..No association was found between placental malaria and either maternal virus load, genital HIV-1 DNA, or HIV-1 RNA. Placental malaria did not correlate with in utero or peripartal transmission of HIV-1... - Comparison of phenotypic and genotypic tropism determination in triple-class-experienced HIV patients eligible for maraviroc treatmentLinos Vandekerckhove
Department of General Internal Medicine, Infectious Diseases and Psychosomatic Medicine, University Hospital Ghent, Ghent, Belgium
J Antimicrob Chemother 66:265-72. 2011..This study evaluated the potential of population genotypic tropism tests (GTTs) in clinical practice, and the correlation with phenotypic tropism tests (PTTs) in patients accessing routine HIV care... - Low efficacy of nevirapine (HIVNET012) in preventing perinatal HIV-1 transmission in a real-life situationAnn Quaghebeur
International Centre for Reproductive Health, Ghent University, Ghent, Belgium
AIDS 18:1854-6. 2004..1%, similar to the 21.7% before the intervention. These data call for further evaluation of the simple nevirapine regimen in field conditions, and underline the need for alter-native strategies... - The latent HIV-1 reservoir in patients undergoing HAART: an archive of pre-HAART drug resistanceAnn Noë
AIDS Reference Laboratory, Ghent University Hospital, De Pintelaan 185, B 9000 Gent, Belgium
J Antimicrob Chemother 55:410-2. 2005..It can be concluded that, as a consequence of the lifelong conservation of this latent reservoir, the potency of drugs for which resistance once developed will remain reduced, even after years of withdrawal of the drug... - Ultra-deep sequencing of HIV-1 reverse transcriptase before start of an NNRTI-based regimen in treatment-naive patientsPeter Messiaen
AIDS Reference Laboratory, Ghent University, De Pintelaan 185 9000 Gent, Belgium
Virology 426:7-11. 2012..Using UDPS, we could not confirm an association between the presence of low minority variants harbouring RT mutations at the start of therapy and primary or secondary therapeutic failure... - Maraviroc: integration of a new antiretroviral drug class into clinical practiceLinos Vandekerckhove
AIDS Reference Centre, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
J Antimicrob Chemother 61:1187-90. 2008..We discuss the challenges associated with the currently available assay, as well as the potential role of alternative assays... - Maraviroc: perspectives for use in antiretroviral-naive HIV-1-infected patientsLinos Vandekerckhove
AIDS Reference Centre, Gent University Hospital, Belgium
J Antimicrob Chemother 63:1087-96. 2009..This paper discusses both the prospective and retrospective analyses of the MERIT data and highlights the impact of these results on daily practice in HIV care... - Placental inflammation and perinatal transmission of HIV-1Fabian Mwanyumba
International Centre for Reproductive Health, Ghent University, Ghent, Belgium
J Acquir Immune Defic Syndr 29:262-9. 2002..We suggest that further research on the role of antimicrobial treatment in the prevention of chorioamnionitis and the reduction of peripartum MTCT needs to be performed... - Correlation of coreceptor usage and disease progressionChris Verhofstede
AIDS Reference Laboratory, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent, Belgium
Curr Opin HIV AIDS 7:432-9. 2012..In this article we provide a historical overview of the events that led to the discovery of the relationship between viral phenotype, coreceptor tropism and pathogenesis... - Association of haptoglobin phenotypes with the development of Kaposi's sarcoma in HIV patientsReinhart Speeckaert
Department of Dermatology, Ghent University Hospital, Ghent, Belgium
Arch Dermatol Res 303:763-9. 2011..001). In contrast, the Hp 2-1 phenotype carried the lowest risk. These findings point to the involvement of Hp phenotypes in the pathogenesis of KS, which may be due to a difference in skin immunosurveillance between the Hp phenotypes... - Prevalence and epidemiology of HIV type 1 drug resistance among newly diagnosed therapy-naive patients in Belgium from 2003 to 2006Jurgen Vercauteren
Rega Institute for Medical Research and University Hospitals, Katholieke Universiteit Leuven, Leuven, Belgium
AIDS Res Hum Retroviruses 24:355-62. 2008..e., zidovudine, lamivudine, and efavirenz. Our results support the implementation of genotypic resistance testing as a standard of care in all treatment-naive patients in Belgium...