Chris Verhofstede

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. pmc Transmitted drug resistance, selection of resistance mutations and moderate antiretroviral efficacy in HIV-2: analysis of the HIV-2 Belgium and Luxembourg database
    Jean Ruelle
    Universite Catholique de Louvain, AIDS Reference Laboratory, Avenue Hippocrate 54 5492, 1200 Bruxelles, Belgium
    BMC Infect Dis 8:21. 2008
  2. pmc HIV-1 V3 envelope deep sequencing for clinical plasma specimens failing in phenotypic tropism assays
    Ina Vandenbroucke
    Tibotec Virco Virology BVBA, Mechelen, Belgium
    AIDS Res Ther 7:4. 2010
  3. pmc Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, Kenya
    Kim Steegen
    AIDS Reference Laboratory, Ghent University, Ghent, Belgium
    AIDS Res Ther 6:12. 2009
  4. doi CXCR4-using HIV type 1 variants are more commonly found in peripheral blood mononuclear cell DNA than in plasma RNA
    Chris Verhofstede
    AIDS Reference Laboratory, Ghent University, Ghent, Belgium
    J Acquir Immune Defic Syndr 50:126-36. 2009
  5. doi Concordance between HIV-1 genotypic coreceptor tropism predictions based on plasma RNA and proviral DNA
    C Verhofstede
    AIDS Reference Laboratory and AIDS Reference Centre, Ghent University and Ghent University Hospital, Ghent, Belgium
    HIV Med 12:544-52. 2011
  6. ncbi Drug-resistant variants that evolve during nonsuppressive therapy persist in HIV-1-infected peripheral blood mononuclear cells after long-term highly active antiretroviral therapy
    Chris Verhofstede
    AIDS Reference Laboratory, Ghent University Hospital, Belgium
    J Acquir Immune Defic Syndr 35:473-83. 2004
  7. doi Viral load assay sensitivity and low level viremia in HAART treated HIV patients
    Chris Verhofstede
    Ghent University, AIDS Reference Laboratory, De Pintelaan 185, B 9000 Ghent, Belgium
    J Clin Virol 47:335-9. 2010
  8. ncbi Detection of drug resistance mutations as a predictor of subsequent virological failure in patients with HIV-1 viral rebounds of less than 1,000 RNA copies/ml
    Chris Verhofstede
    AIDS Reference Laboratory, Ghent University Hospital, Belgium
    J Med Virol 79:1254-60. 2007
  9. ncbi A sensitive in-house RT-PCR genotyping system for combined detection of plasma HIV-1 and assessment of drug resistance
    Kim Steegen
    International Centre for Reproductive Health, University Hospital, Ghent, Belgium
    J Virol Methods 133:137-45. 2006
  10. doi Impact of Delta 32-CCR5 heterozygosity on HIV-1 genetic evolution and variability--a study of 4 individuals infected with closely related HIV-1 strains
    Kristen Chalmet
    AIDS Reference Laboratory, Ghent University and Ghent University Hospital, De Pintelaan, 185 Block A, B 9000 Gent, Belgium
    Virology 379:213-22. 2008

Collaborators

Detail Information

Publications32

  1. pmc Transmitted drug resistance, selection of resistance mutations and moderate antiretroviral efficacy in HIV-2: analysis of the HIV-2 Belgium and Luxembourg database
    Jean Ruelle
    Universite Catholique de Louvain, AIDS Reference Laboratory, Avenue Hippocrate 54 5492, 1200 Bruxelles, Belgium
    BMC Infect Dis 8:21. 2008
    ..Guidelines established for the treatment of HIV-1 infection and genotype interpretation do not apply for HIV-2. Data about antiretroviral (ARV) drug efficacy and resistance mutations is scarce...
  2. pmc HIV-1 V3 envelope deep sequencing for clinical plasma specimens failing in phenotypic tropism assays
    Ina Vandenbroucke
    Tibotec Virco Virology BVBA, Mechelen, Belgium
    AIDS Res Ther 7:4. 2010
    ..While good concordance was observed between gp120 phenotyping and prediction of R5-tropic virus, the results suggest that accurate prediction of X4-tropic virus would require further algorithm development...
  3. pmc Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, Kenya
    Kim Steegen
    AIDS Reference Laboratory, Ghent University, Ghent, Belgium
    AIDS Res Ther 6:12. 2009
    ....
  4. doi CXCR4-using HIV type 1 variants are more commonly found in peripheral blood mononuclear cell DNA than in plasma RNA
    Chris Verhofstede
    AIDS Reference Laboratory, Ghent University, Ghent, Belgium
    J Acquir Immune Defic Syndr 50:126-36. 2009
    ..To compare the distribution of R5-like and X4-like HIV-1 envelope sequences in plasma and peripheral blood mononuclear cell (PBMC)...
  5. doi Concordance between HIV-1 genotypic coreceptor tropism predictions based on plasma RNA and proviral DNA
    C Verhofstede
    AIDS Reference Laboratory and AIDS Reference Centre, Ghent University and Ghent University Hospital, Ghent, Belgium
    HIV Med 12:544-52. 2011
    ..The aim of the study was to evaluate the use of proviral DNA as a source of viral genetic material for genotypic coreceptor tropism testing (GTT)...
  6. ncbi Drug-resistant variants that evolve during nonsuppressive therapy persist in HIV-1-infected peripheral blood mononuclear cells after long-term highly active antiretroviral therapy
    Chris Verhofstede
    AIDS Reference Laboratory, Ghent University Hospital, Belgium
    J Acquir Immune Defic Syndr 35:473-83. 2004
    ..These data clearly show that virus variants that are able to replicate for a certain period enter the latent reservoir and remain archived in the PBMCs for a very long period...
  7. doi Viral load assay sensitivity and low level viremia in HAART treated HIV patients
    Chris Verhofstede
    Ghent University, AIDS Reference Laboratory, De Pintelaan 185, B 9000 Ghent, Belgium
    J Clin Virol 47:335-9. 2010
    ..The significance of a viral load between 20 and 50 copies/mL remains unclear...
  8. ncbi Detection of drug resistance mutations as a predictor of subsequent virological failure in patients with HIV-1 viral rebounds of less than 1,000 RNA copies/ml
    Chris Verhofstede
    AIDS Reference Laboratory, Ghent University Hospital, Belgium
    J Med Virol 79:1254-60. 2007
    ..Most importantly, detection of resistance mutations in viral rebound samples was predictive of subsequent virological failure...
  9. ncbi A sensitive in-house RT-PCR genotyping system for combined detection of plasma HIV-1 and assessment of drug resistance
    Kim Steegen
    International Centre for Reproductive Health, University Hospital, Ghent, Belgium
    J Virol Methods 133:137-45. 2006
    ..The assay enables the accurate follow-up of patients under treatment at a significantly reduced cost compared to the currently available commercial assays for viral load assessment and genotyping...
  10. doi Impact of Delta 32-CCR5 heterozygosity on HIV-1 genetic evolution and variability--a study of 4 individuals infected with closely related HIV-1 strains
    Kristen Chalmet
    AIDS Reference Laboratory, Ghent University and Ghent University Hospital, De Pintelaan, 185 Block A, B 9000 Gent, Belgium
    Virology 379:213-22. 2008
    ....
  11. pmc Feasibility of detecting human immunodeficiency virus type 1 drug resistance in DNA extracted from whole blood or dried blood spots
    Kim Steegen
    International Centre for Reproductive Health, Ghent University, Belgium
    J Clin Microbiol 45:3342-51. 2007
    ..The use of DNA extracted from whole blood or DBS for the detection of archived drug resistance mutations deserves further study...
  12. pmc Diversity of the human immunodeficiency virus type 1 (HIV-1) env sequence after vertical transmission in mother-child pairs infected with HIV-1 subtype A
    Chris Verhofstede
    AIDS Reference Laboratory, Ghent University Hospital, Ghent, Belgium
    J Virol 77:3050-7. 2003
    ..Phylogenetic tree topologies argue against selection processes as the major mechanism driving mother-to-child transmission but support the hypothesis that virus variability is mainly driven by the inoculum level and/or exposure time...
  13. ncbi Evaluation of two commercially available alternatives for HIV-1 viral load testing in resource-limited settings
    Kim Steegen
    International Centre for Reproductive Health, University Hospital, Ghent, Belgium
    J Virol Methods 146:178-87. 2007
    ..Hence, false positive results and the need for an expensive PCR instrument for the Generic HIV viral load assays still limit the implementation of these tests in less equipped, less experienced laboratories...
  14. pmc Epidemiological study of phylogenetic transmission clusters in a local HIV-1 epidemic reveals distinct differences between subtype B and non-B infections
    Kristen Chalmet
    Ghent University, Belgium
    BMC Infect Dis 10:262. 2010
    ....
  15. doi Presence of CXCR4-using HIV-1 in patients with recently diagnosed infection: correlates and evidence for transmission
    Kristen Chalmet
    AIDS Reference Laboratory, Ghent University, Belgium
    J Infect Dis 205:174-84. 2012
    ..The prevalence and correlates of CXCR4-use in recently diagnosed patients and the impact of X4/DM transmission remain largely unknown...
  16. ncbi Placental malaria and perinatal transmission of human immunodeficiency virus type 1
    Ingrid Inion
    International Centre for Reproductive Health, Ghent University, Ghent, Belgium
    J Infect Dis 188:1675-8. 2003
    ..No association was found between placental malaria and either maternal virus load, genital HIV-1 DNA, or HIV-1 RNA. Placental malaria did not correlate with in utero or peripartal transmission of HIV-1...
  17. pmc Comparison of phenotypic and genotypic tropism determination in triple-class-experienced HIV patients eligible for maraviroc treatment
    Linos Vandekerckhove
    Department of General Internal Medicine, Infectious Diseases and Psychosomatic Medicine, University Hospital Ghent, Ghent, Belgium
    J Antimicrob Chemother 66:265-72. 2011
    ..This study evaluated the potential of population genotypic tropism tests (GTTs) in clinical practice, and the correlation with phenotypic tropism tests (PTTs) in patients accessing routine HIV care...
  18. ncbi Low efficacy of nevirapine (HIVNET012) in preventing perinatal HIV-1 transmission in a real-life situation
    Ann Quaghebeur
    International Centre for Reproductive Health, Ghent University, Ghent, Belgium
    AIDS 18:1854-6. 2004
    ..1%, similar to the 21.7% before the intervention. These data call for further evaluation of the simple nevirapine regimen in field conditions, and underline the need for alter-native strategies...
  19. pmc Frequency and predictors of HIV-1 co-receptor switch in treatment naive patients
    Virginie Mortier
    AIDS Reference Laboratory, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Gent, Belgium
    PLoS ONE 8:e80259. 2013
    ..Determination of HIV-1 co-receptor use is a necessity before initiation of a CCR5 antagonist but the longevity of a CCR5-use prediction remains unknown...
  20. ncbi The latent HIV-1 reservoir in patients undergoing HAART: an archive of pre-HAART drug resistance
    Ann Noë
    AIDS Reference Laboratory, Ghent University Hospital, De Pintelaan 185, B 9000 Gent, Belgium
    J Antimicrob Chemother 55:410-2. 2005
    ..It can be concluded that, as a consequence of the lifelong conservation of this latent reservoir, the potency of drugs for which resistance once developed will remain reduced, even after years of withdrawal of the drug...
  21. doi Touchdown digital polymerase chain reaction for quantification of highly conserved sequences in the HIV-1 genome
    Ward De Spiegelaere
    Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium
    Anal Biochem 439:201-3. 2013
    ....
  22. doi Ultra-deep sequencing of HIV-1 reverse transcriptase before start of an NNRTI-based regimen in treatment-naive patients
    Peter Messiaen
    AIDS Reference Laboratory, Ghent University, De Pintelaan 185 9000 Gent, Belgium
    Virology 426:7-11. 2012
    ..Using UDPS, we could not confirm an association between the presence of low minority variants harbouring RT mutations at the start of therapy and primary or secondary therapeutic failure...
  23. doi Maraviroc: integration of a new antiretroviral drug class into clinical practice
    Linos Vandekerckhove
    AIDS Reference Centre, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    J Antimicrob Chemother 61:1187-90. 2008
    ..We discuss the challenges associated with the currently available assay, as well as the potential role of alternative assays...
  24. doi Maraviroc: perspectives for use in antiretroviral-naive HIV-1-infected patients
    Linos Vandekerckhove
    AIDS Reference Centre, Gent University Hospital, Belgium
    J Antimicrob Chemother 63:1087-96. 2009
    ..This paper discusses both the prospective and retrospective analyses of the MERIT data and highlights the impact of these results on daily practice in HIV care...
  25. pmc Socio-economic, behavioural, (neuro)psychological and clinical determinants of HRQoL in people living with HIV in Belgium: a pilot study
    Sophie Degroote
    Department of General Internal Medicine, Infectious Diseases and Psychosomatics, Ghent University Hospital, Ghent, Belgium
    J Int AIDS Soc 16:18643. 2013
    ..The purpose of this study was to identify socio-economic, behavioural, (neuro)psychological and clinical determinants of HRQoL among people living with HIV (PLHIV)...
  26. ncbi Placental inflammation and perinatal transmission of HIV-1
    Fabian Mwanyumba
    International Centre for Reproductive Health, Ghent University, Ghent, Belgium
    J Acquir Immune Defic Syndr 29:262-9. 2002
    ..We suggest that further research on the role of antimicrobial treatment in the prevention of chorioamnionitis and the reduction of peripartum MTCT needs to be performed...
  27. doi Polyfunctional CD4(+) T cell responses in HIV-1-infected viral controllers compared with those in healthy recipients of an adjuvanted polyprotein HIV-1 vaccine
    Eva Van Braeckel
    Center for Vaccinology, Ghent University and Hospital, Ghent, Belgium
    Vaccine 31:3739-46. 2013
    ....
  28. pmc Characterization of LEDGF/p75 genetic variants and association with HIV-1 disease progression
    Peter Messiaen
    HIV Translational Research Unit, Department of General Internal Medicine and Infectious Diseases, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 7:e50204. 2012
    ..This study evaluated the relation between LEDGF/p75 genetic variation, mRNA expression and HIV-1 disease progression in order to guide future clinical use of LEDGINs...
  29. doi Correlation of coreceptor usage and disease progression
    Chris Verhofstede
    AIDS Reference Laboratory, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent, Belgium
    Curr Opin HIV AIDS 7:432-9. 2012
    ..In this article we provide a historical overview of the events that led to the discovery of the relationship between viral phenotype, coreceptor tropism and pathogenesis...
  30. doi Association of haptoglobin phenotypes with the development of Kaposi's sarcoma in HIV patients
    Reinhart Speeckaert
    Department of Dermatology, Ghent University Hospital, Ghent, Belgium
    Arch Dermatol Res 303:763-9. 2011
    ..001). In contrast, the Hp 2-1 phenotype carried the lowest risk. These findings point to the involvement of Hp phenotypes in the pathogenesis of KS, which may be due to a difference in skin immunosurveillance between the Hp phenotypes...
  31. doi Transmission network of an HIV type 1 strain with K103N in young Belgian patients from different risk groups
    Jean Ruelle
    1 Université catholique de Louvain, AIDS Reference Laboratory, IREC, Brussels, Belgium
    AIDS Res Hum Retroviruses 29:1306-9. 2013
    ..The identification of this transmission network advocates for local prevention reinforcement and underscores the need for continuous TDR monitoring. The spread of NNRTI TDR could affect ARV initiation schemes and prophylaxis strategies...
  32. doi Prevalence and epidemiology of HIV type 1 drug resistance among newly diagnosed therapy-naive patients in Belgium from 2003 to 2006
    Jurgen Vercauteren
    Rega Institute for Medical Research and University Hospitals, Katholieke Universiteit Leuven, Leuven, Belgium
    AIDS Res Hum Retroviruses 24:355-62. 2008
    ..e., zidovudine, lamivudine, and efavirenz. Our results support the implementation of genotypic resistance testing as a standard of care in all treatment-naive patients in Belgium...