Rita Verhelst

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. pmc Antibiotic susceptibility of Atopobium vaginae
    Ellen De Backer
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Infect Dis 6:51. 2006
  2. pmc Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners
    Ellen De Backer
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
    BMC Microbiol 7:115. 2007
  3. pmc Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests
    Vicky Jespers
    Department of Public Health, ITM HIV AIDS Centre, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium
    BMC Microbiol 12:83. 2012
  4. pmc The vaginal microflora in relation to gingivitis
    Rutger Persson
    Department of Periodontology, Div of Oral Microbiology, University of Berne, Berne, Switzerland
    BMC Infect Dis 9:6. 2009
  5. pmc Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species
    Tim Stakenborg
    Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium
    BMC Infect Dis 5:46. 2005
  6. pmc Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microflora
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
    BMC Microbiol 5:61. 2005
  7. pmc Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 4:16. 2004
  8. pmc Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women
    Nabil A El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 10:285. 2010
  9. pmc Longitudinal qPCR study of the dynamics of L. crispatus, L. iners, A. vaginae, (sialidase positive) G. vaginalis, and P. bivia in the vagina
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 7:e45281. 2012
  10. pmc Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cycles
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 6:e28180. 2011

Collaborators

Detail Information

Publications38

  1. pmc Antibiotic susceptibility of Atopobium vaginae
    Ellen De Backer
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Infect Dis 6:51. 2006
    ..Thus far the four isolates of this fastidious micro-organism were found to be highly resistant to metronidazole and susceptible for clindamycin, two antibiotics preferred for the treatment of BV...
  2. pmc Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners
    Ellen De Backer
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
    BMC Microbiol 7:115. 2007
    ..iners and L. jensenii to obtain a quantitative non culture-based determination of these species in 71 vaginal samples from 32 pregnant and 28 non-pregnant women aged between 18 and 45 years...
  3. pmc Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests
    Vicky Jespers
    Department of Public Health, ITM HIV AIDS Centre, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium
    BMC Microbiol 12:83. 2012
    ..We also explored differences in qPCR data between these healthy women and an 'at risk' clinic population of Caucasian, African and Asian women with and without bacterial vaginosis (BV), as defined by the Nugent score...
  4. pmc The vaginal microflora in relation to gingivitis
    Rutger Persson
    Department of Periodontology, Div of Oral Microbiology, University of Berne, Berne, Switzerland
    BMC Infect Dis 9:6. 2009
    ..Gingivitis has been linked to adverse pregnancy outcome (APO). Bacterial vaginosis (BV) has been associated with APO. We assessed if bacterial counts in BV is associated with gingivitis suggesting a systemic infectious susceptibilty...
  5. pmc Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species
    Tim Stakenborg
    Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium
    BMC Infect Dis 5:46. 2005
    ..We studied the applicability of amplified rDNA restriction analysis (ARDRA) for the identification of all 116 acknowledged Mycoplasma species and subspecies...
  6. pmc Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microflora
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
    BMC Microbiol 5:61. 2005
    ....
  7. pmc Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 4:16. 2004
    ..Here, we report on the results obtained by combining culture and PCR-based methods to characterize the normal and disturbed vaginal microflora...
  8. pmc Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women
    Nabil A El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 10:285. 2010
    ..However, this procedure may require 48 h to complete. We compared different sampling and culture techniques for the detection of GBS...
  9. pmc Longitudinal qPCR study of the dynamics of L. crispatus, L. iners, A. vaginae, (sialidase positive) G. vaginalis, and P. bivia in the vagina
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 7:e45281. 2012
    ..To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles...
  10. pmc Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cycles
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 6:e28180. 2011
    ....
  11. pmc Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora
    Nabil Abdullah El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 9:167. 2009
    ..The vaginal microflora is important for maintaining vaginal health and preventing infections of the reproductive tract. The rectum has been suggested as the major source for the colonisation of the vaginal econiche...
  12. doi request reprint Strong correspondence in bacterial loads between the vagina and rectum of pregnant women
    Nabil Abdullah El Aila
    Laboratory of Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, 3BlokA, 185 De Pintelaan, 9000 Ghent, Flanders, Belgium
    Res Microbiol 162:506-13. 2011
    ....
  13. pmc Genotyping of Streptococcus agalactiae (group B streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancy
    Nabil Abdullah El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 9:153. 2009
    ..Here we compared different culture media for GBS detection and we compared the occurrence of different genotypes and serotypes of GBS isolates from the vagina and rectum...
  14. pmc Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    BMC Microbiol 9:116. 2009
    ....
  15. pmc The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays
    Nabil Abdullah El Aila
    Laboratory Bacteriology Research, Department of Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 10:104. 2010
    ..Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge...
  16. pmc Microflora of the penile skin-lined neovagina of transsexual women
    Steven Weyers
    Department of Obstetrics and Gynaecology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 9:102. 2009
    ..Secondary objectives were to describe possible correlations of this microflora with multiple patients' characteristics, such as sexual orientation, the incidence of vaginal irritation and malodorous vaginal discharge...
  17. ncbi request reprint Modified classification of Gram-stained vaginal smears to predict spontaneous preterm birth: a prospective cohort study
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Am J Obstet Gynecol 196:528.e1-6. 2007
    ..The purpose of this study was to identify women at risk of spontaneous preterm birth through a 4-category Gram-stained vaginal smear method, not restricted to bacterial vaginosis...
  18. pmc Comparison of five genotypic techniques for identification of optochin-resistant pneumococcus-like isolates
    Rita Verhelst
    Department of Chemistry, Microbiology and Immunology, Ghent University Hospital, Gent, Belgium
    J Clin Microbiol 41:3521-5. 2003
    ..We found the psaA-specific PCR to be the genotypic technique best suited for the identification of genuine pneumococci and optochin-resistant pneumococci...
  19. doi request reprint Chlamydia trachomatis infection in fertile and subfertile women in Rwanda: prevalence and diagnostic significance of IgG and IgA antibodies testing
    Claude Mambo Muvunyi
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    Hum Reprod 26:3319-26. 2011
    ..The objective of the present study was to determine the prevalence of C. trachomatis and evaluate the diagnostic utility of serological markers namely anti-C. trachomatis IgG and IgA antibodies in women attending an infertility clinic...
  20. ncbi request reprint Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium
    Am J Obstet Gynecol 191:1130-2. 2004
    ..Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora...
  21. doi request reprint A pilot study evaluating the safety of vaginal administration of a multi-particulate pellet formulation
    Guido Lopes Dos Santos Santiago
    Laboratory of Bacteriology Research LBR, Ghent University, Ghent, Belgium
    Eur J Pharm Biopharm 73:399-403. 2009
    ..Quantitative evaluation of the effect caused by vaginal administration of gelatin capsules loaded with starch pellets and lyophilized powder, respectively, on vaginal pH and microflora...
  22. pmc Elevated soluble triggering receptor expressed on myeloid cells (sTREM)-1 levels in maternal serum during term and preterm labor
    Inge Tency
    Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 8:e56050. 2013
    ..Few studies have investigated serum sTREM-1 expression during preterm labor. Therefore, the purpose of this study was to assess sTREM-1 concentrations in maternal serum during term and preterm labor...
  23. pmc Bacteremic infection with Pantoea ananatis
    Thierry de Baere
    Department of Clinical Chemistry, Microbiology and Immunology, Blok A, University Hospital, Ghent, Belgium thierry debaire ugent be
    J Clin Microbiol 42:4393-5. 2004
    ..Subsequent sequencing of the 16S rRNA gene revealed a final identification as Pantoea ananatis. The patient was given intravenous and oral therapy with piperacillin-tazobactam and ofloxacin and recovered completely from his infection...
  24. pmc Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory
    Thierry de Baere
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 2:4. 2002
    ..identification of all mycobacterial species at once, instead of attempting direct PCR based detection from clinical samples of M. tuberculosis only...
  25. doi request reprint Bacterial vaginosis: an update on diagnosis and treatment
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
    Expert Rev Anti Infect Ther 7:1109-24. 2009
    ..As an adjuvant to standard treatment with antibiotics, alternative treatments with antiseptics and disinfectants, vaginal-acidifying and -buffering agents, and probiotics hold some promise for long-term prevention...
  26. pmc Imbalances between matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in maternal serum during preterm labor
    Inge Tency
    Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 7:e49042. 2012
    ..Therefore, the purpose of this study was to determine serum concentrations of MMP-3, MMP-9 and all four TIMPs as well as MMP:TIMP ratios during term and preterm labor...
  27. pmc The epidemiology of bacterial vaginosis in relation to sexual behaviour
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    BMC Infect Dis 10:81. 2010
    ..We therefore made a critical appraisal of the literature on BV in relation to sexual behaviour...
  28. doi request reprint Terminal restriction fragment length polymorphism analysis of the microflora in necrotic teeth of patients irradiated in the head and neck region
    Geert M G Hommez
    Department of Restorative Dentistry and Endodontology, Ghent University Hospital, Gent, Belgium
    J Endod 34:1048-51. 2008
    ..Capnocytophaga spp., and Actinomyces spp.) found in group A were not found in group B. In conclusion, the diversity of root canal microflora increases significantly after head-neck radiotherapy...
  29. doi request reprint Group A streptococcal vaginitis: an unrecognized cause of vaginal symptoms in adult women
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Arch Gynecol Obstet 284:95-8. 2011
    ..We sought to give an overview of the epidemiology, risk factors, symptoms, signs, and treatment of GAS vaginitis in adult women...
  30. doi request reprint Gene polymorphisms of Toll-like and related recognition receptors in relation to the vaginal carriage of Gardnerella vaginalis and Atopobium vaginae
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185, B 9000 Ghent, Belgium
    J Reprod Immunol 79:163-73. 2009
    ..Our data suggest that some degree of genetic susceptibility involving pathogen recognition may occur with the key bacterial vaginosis organism, A. vaginae...
  31. pmc Shared genotypes of Achromobacter xylosoxidans strains isolated from patients at a cystic fibrosis rehabilitation center
    Sabine Van Daele
    Department of Pediatric Pulmonology, University Hospital Ghent, De Pintelaan 185, B9000 Ghent, Belgium
    J Clin Microbiol 43:2998-3002. 2005
    ..aeruginosa, as well as two clusters of 4 and 10 patients, respectively, colonized with genotypically identical Achromobacter xylosoxidans strains, were discovered...
  32. ncbi request reprint Digitized fluorescent RFLP analysis (fRFLP) as a universal method for comparing genomes of culturable dsDNA viruses: application to bacteriophages
    Maia Merabishvili
    George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
    Res Microbiol 158:572-81. 2007
    ....
  33. pmc Antiseptics and disinfectants for the treatment of bacterial vaginosis: a systematic review
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185, B 9000, Ghent, Belgium
    BMC Infect Dis 12:148. 2012
    ..The study objective was to assess the available data on efficacy and tolerability of antiseptics and disinfectants in treating bacterial vaginosis (BV)...
  34. pmc Association between bacterial vaginosis and cervical intraepithelial neoplasia: systematic review and meta-analysis
    Evy Gillet
    International Centre for Reproductive Health ICRH, Ghent University, Ghent, Belgium
    PLoS ONE 7:e45201. 2012
    ..Previous studies examining the relationship between BV and cervical intra-epithelial neoplasia (CIN) provided inconsistent and conflicting results. The aim of this study is to clarify the association between these two conditions...
  35. doi request reprint Evaluation of a new multiplex polymerase chain reaction assay STDFinder for the simultaneous detection of 7 sexually transmitted disease pathogens
    Claude Mambo Muvunyi
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    Diagn Microbiol Infect Dis 71:29-37. 2011
    ....
  36. pmc Evaluation of tRNA gene PCR for identification of mollicutes
    Tim Stakenborg
    Veterinary and Agrochemical Research Center, Brussels, Belgium
    J Clin Microbiol 43:4558-66. 2005
    ..In conclusion, we found tRNA gene PCR to be a rapid and discriminatory method to correctly identify a large collection of different species of the class of Mollicutes and to recognize not-yet-described groups...
  37. ncbi request reprint Streptococcus cristatus isolated from a resected heart valve and blood cultures: case reports and application of phenotypic and genotypic techniques for identification
    Charlotte Matthys
    Dienst Klinische Chemie, Microbiologie en Immunologie, Universitair Ziekenhuis Gent, De Pintelaan 185, B 9000 Gent, Belgie
    Acta Clin Belg 61:196-200. 2006
    ..Using tDNA-PCR, it is possible to identify to species level and to differentiate between species of the viridans group...
  38. ncbi request reprint Identification of Lactobacillus species using tDNA-PCR
    Margo Baele
    Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    J Microbiol Methods 50:263-71. 2002
    ..gallinarum and L. helveticus). Inclusion of the resulting fingerprints in a numerical database containing fingerprints of numerous other Gram-positive and Gram-negative species makes the identification of unknown strains possible...