Research Topics
Genomes and Genes | Mario VaneechoutteSummaryAffiliation: Ghent University Country: Belgium Publications
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Publications
The vaginal microflora in relation to gingivitisRutger Persson
Department of Periodontology, Div of Oral Microbiology, University of Berne, Berne, Switzerland
BMC Infect Dis 9:6. 2009..Gingivitis has been linked to adverse pregnancy outcome (APO). Bacterial vaginosis (BV) has been associated with APO. We assessed if bacterial counts in BV is associated with gingivitis suggesting a systemic infectious susceptibilty...- Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma speciesTim Stakenborg
Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium
BMC Infect Dis 5:46. 2005..We studied the applicability of amplified rDNA restriction analysis (ARDRA) for the identification of all 116 acknowledged Mycoplasma species and subspecies... - Exploring the evolutionary dynamics of plasmids: the Acinetobacter pan-plasmidomeMarco Fondi
Laboratory of Microbial and Molecular Evolution, Dept, of Evolutionary Biology, via Romana 17 19, University of Florence, I 50125 Florence, Italy
BMC Evol Biol 10:59. 2010..Given their importance for conferring adaptive traits to the host organisms, the interest in plasmid sequencing is growing and now many complete plasmid sequences are available online... - Naturally transformable Acinetobacter sp. strain ADP1 belongs to the newly described species Acinetobacter baylyiMario Vaneechoutte
Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Blok A, De Pintelaan 185, 9000 Gent, Belgium
Appl Environ Microbiol 72:932-6. 2006.... 
Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp. novMario Vaneechoutte
Laboratory Bacteriology Research LBR, Department of Clinical Chemistry, Ghent University Hospital, 9000 Gent, Belgium
Int J Syst Evol Microbiol 59:1376-81. 2009..The five strains represent a novel species for which the name Acinetobacter venetianus sp. nov. is proposed. The type strain is RAG-1(T) (=ATCC 31012(T)=CCUG 45561(T)=LMG 19082(T)=LUH 3904(T)=NIPH 1925(T))...
Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-cMario Vaneechoutte
Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
Int J Syst Evol Microbiol 57:2623-8. 2007..hispanicum. The DNA G+C content of strain NF802T is 36.5 mol%. The name Chryseobacterium hominis sp. nov. is proposed for this taxon, with type strain NF802T (=CCUG 52711T=CIP 109415T)...- From the primordial soup to the latest universal common ancestorMario Vaneechoutte
Laboratory Bacteriology Research, Faculty Medicine and Health Sciences, University of Ghent, 3 Blok A, De Pintelaan 185, 9000 Gent, Belgium
Res Microbiol 160:437-40. 2009.... - Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. novMario Vaneechoutte
Department of Clinical Chemistry, Microbiology and Immunology, Blok A, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
Int J Syst Evol Microbiol 54:317-27. 2004..eutropha lineage should be classified in a novel genus, Wautersia gen. nov. Finally, based on the literature and new DNA-DNA hybridization data, it is proposed that Pseudomonas syzygii should be renamed Ralstonia syzygii comb. nov... - Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986Mario Vaneechoutte
Department of Clinical Chemistry, Microbiology and Immunology, University Hospital, Blok A, B 9000 Gent, Belgium
Int J Syst Evol Microbiol 58:937-40. 2008..These findings were corroborated for a second representative of the A. grimontii type strain, DSM 14968T. These data confirm that Acinetobacter grimontii is a later heterotypic synonym of Acinetobacter junii... - Digitized fluorescent RFLP analysis (fRFLP) as a universal method for comparing genomes of culturable dsDNA viruses: application to bacteriophagesMaia Merabishvili
George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
Res Microbiol 158:572-81. 2007.... - Longitudinal qPCR study of the dynamics of L. crispatus, L. iners, A. vaginae, (sialidase positive) G. vaginalis, and P. bivia in the vaginaGuido Lopes Dos Santos Santiago
Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
PLoS ONE 7:e45281. 2012..To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles... - Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant womenNabil A El Aila
Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
BMC Infect Dis 10:285. 2010..However, this procedure may require 48 h to complete. We compared different sampling and culture techniques for the detection of GBS... - Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cyclesGuido Lopes Dos Santos Santiago
Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
PLoS ONE 6:e28180. 2011.... - Genotyping of Streptococcus agalactiae (group B streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancyNabil Abdullah El Aila
Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
BMC Infect Dis 9:153. 2009..Here we compared different culture media for GBS detection and we compared the occurrence of different genotypes and serotypes of GBS isolates from the vagina and rectum... - Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microfloraNabil Abdullah El Aila
Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
BMC Infect Dis 9:167. 2009..The vaginal microflora is important for maintaining vaginal health and preventing infections of the reproductive tract. The rectum has been suggested as the major source for the colonisation of the vaginal econiche... - Comparison of culture with two different qPCR assays for detection of rectovaginal carriage of Streptococcus agalactiae (group B streptococci) in pregnant womenNabil Abdullah El Aila
Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, 3BlokA, 185 De Pintelaan, 9000 Ghent, Flanders, Belgium
Res Microbiol 162:499-505. 2011..qPCR after Lim broth enrichment further increased the percentage of GBS-positive women, as detected by direct qPCR, from 27 to 33%, although the bacterial inoculum was low for these subjects... - Antibiotic susceptibility of Atopobium vaginaeEllen De Backer
Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
BMC Infect Dis 6:51. 2006..Thus far the four isolates of this fastidious micro-organism were found to be highly resistant to metronidazole and susceptible for clindamycin, two antibiotics preferred for the treatment of BV... - Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patientsPieter Deschaght
Laboratory for Bacteriology Research, Ghent University Hospital, University of Ghent, Ghent, Belgium
BMC Microbiol 9:244. 2009..aeruginosa oprL gene. In addition, we compared five DNA-extraction protocols... - Bacteremic infection with Pantoea ananatisThierry de Baere
Department of Clinical Chemistry, Microbiology and Immunology, Blok A, University Hospital, Ghent, Belgium. thierry.debaire.ugent.be
J Clin Microbiol 42:4393-5. 2004..Subsequent sequencing of the 16S rRNA gene revealed a final identification as Pantoea ananatis. The patient was given intravenous and oral therapy with piperacillin-tazobactam and ofloxacin and recovered completely from his infection... - Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. inersEllen De Backer
Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
BMC Microbiol 7:115. 2007..iners and L. jensenii to obtain a quantitative non culture-based determination of these species in 71 vaginal samples from 32 pregnant and 28 non-pregnant women aged between 18 and 45 years... - Strong correspondence in bacterial loads between the vagina and rectum of pregnant womenNabil Abdullah El Aila
Laboratory of Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, 3BlokA, 185 De Pintelaan, 9000 Ghent, Flanders, Belgium
Res Microbiol 162:506-13. 2011.... - Repetitive-DNA-element PCR fingerprinting and antibiotic resistance of pan-European multi-resistant Acinetobacter baumannii clone III strainsGeert Huys
Laboratory of Microbiology and BCCM LMG Bacteria Collection, Ghent University, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
J Med Microbiol 54:851-6. 2005.... - tDNA-PCR followed by automated fluorescent capillary electrophoresis for identification of bacterial speciesMargo Baele
Laboratory for Veterinary Microbiology, Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, 9000 Gent, Belgium
Cold Spring Harb Protoc 2009:pdb.prot5196. 2009..g., after 16S rRNA gene sequence determination) can be added to expand the database further. This protocol describes tDNA-PCR, followed by automated fluorescent capillary electrophoresis to identify bacterial species... - The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assaysNabil Abdullah El Aila
Laboratory Bacteriology Research, Department of Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
BMC Infect Dis 10:104. 2010..Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge... - Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microfloraRita Verhelst
Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
BMC Microbiol 5:61. 2005.... - Genotype based evaluation of Pseudomonas aeruginosa eradication treatment success in cystic fibrosis patientsPetra Schelstraete
Cystic Fibrosis Center Gent, Ghent University Hospital, 9000 Gent, Belgium
J Cyst Fibros 9:99-103. 2010..We followed cystic fibrosis patients after a first ever isolation of P. aeruginosa and evaluated the P. aeruginosa-free time period after eradication therapy... - Shared genotypes of Achromobacter xylosoxidans strains isolated from patients at a cystic fibrosis rehabilitation centerSabine Van Daele
Department of Pediatric Pulmonology, University Hospital Ghent, De Pintelaan 185, B9000 Ghent, Belgium
J Clin Microbiol 43:2998-3002. 2005..aeruginosa, as well as two clusters of 4 and 10 patients, respectively, colonized with genotypically identical Achromobacter xylosoxidans strains, were discovered... - Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microfloraHans Verstraelen
Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
BMC Microbiol 9:116. 2009.... - Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patientsPieter Deschaght
Laboratory for Bacteriology Research, Ghent University Hospital, Ghent University, Ghent, Belgium
BMC Microbiol 10:245. 2010..In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients... - Bacteremia due to Moraxella atlantae in a cancer patientThierry de Baere
Department of Microbiology, Ghent University Hospital, Ghent, Belgium Thierry De
J Clin Microbiol 40:2693-5. 2002..atlantae, M. lincolnii, and M. osloensis might constitute three separate genera within the MORAXELLACEAE: After treatment with amoxicillin-clavulanic acid for 2 days, fever subsided and the patient was dismissed... - Streptococcus cristatus isolated from a resected heart valve and blood cultures: case reports and application of phenotypic and genotypic techniques for identificationCharlotte Matthys
Dienst Klinische Chemie, Microbiologie en Immunologie, Universitair Ziekenhuis Gent, De Pintelaan 185, B 9000 Gent, Belgie
Acta Clin Belg 61:196-200. 2006..Using tDNA-PCR, it is possible to identify to species level and to differentiate between species of the viridans group... - Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2)Thierry de Baere
Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Belgium
BMC Microbiol 2:21. 2002..Therefore, we evaluated the feasability of PCR-based amplification of the Internally Transcribed Spacer region 2, followed by fragment size analysis on the ABI Prism 310 for the identification of clinically important yeasts... - Microflora of the penile skin-lined neovagina of transsexual womenSteven Weyers
Department of Obstetrics and Gynaecology, Ghent University Hospital, Ghent, Belgium
BMC Microbiol 9:102. 2009..Secondary objectives were to describe possible correlations of this microflora with multiple patients' characteristics, such as sexual orientation, the incidence of vaginal irritation and malodorous vaginal discharge... - Comparison of five genotypic techniques for identification of optochin-resistant pneumococcus-like isolatesRita Verhelst
Department of Chemistry, Microbiology and Immunology, Ghent University Hospital, Gent, Belgium
J Clin Microbiol 41:3521-5. 2003..We found the psaA-specific PCR to be the genotypic technique best suited for the identification of genuine pneumococci and optochin-resistant pneumococci... - Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosisRita Verhelst
Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
BMC Microbiol 4:16. 2004..Here, we report on the results obtained by combining culture and PCR-based methods to characterize the normal and disturbed vaginal microflora... - Study of the intra- and interlaboratory reproducibility of partial single base C-sequencing of the 16S rRNA gene and its applicability for the identification of members of the genus StreptococcusVirginie Storms
Department of Biochemistry, Physiology and Microbiology, Faculty of Sciences, University of Ghent, Gent, Belgium
Syst Appl Microbiol 25:52-9. 2002.... - Extended-Spectrum beta-lactamase (ESBL) producing Enterobacter aerogenes phenotypically misidentified as Klebsiella pneumoniae or K. terrigenaGeert Claeys
Department of Microbiology, Ghent University Hospital, Ghent, Belgium
BMC Microbiol 4:49. 2004..Here we report that genotypically unrelated strains of E. aerogenes can be misidentified as K. pneumoniae by routine laboratories using standard biochemical identification and using identification automates... - Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosisHans Verstraelen
Department of Obstetrics and Gynaecology, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium
Am J Obstet Gynecol 191:1130-2. 2004..Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora... - Modified classification of Gram-stained vaginal smears to predict spontaneous preterm birth: a prospective cohort studyHans Verstraelen
Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
Am J Obstet Gynecol 196:528.e1-6. 2007..The purpose of this study was to identify women at risk of spontaneous preterm birth through a 4-category Gram-stained vaginal smear method, not restricted to bacterial vaginosis... - Distribution of tetracycline resistance genes in genotypically related and unrelated multiresistant Acinetobacter baumannii strains from different European hospitalsGeert Huys
Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, Ghent, Belgium
Res Microbiol 156:348-55. 2005..Collectively, our phenotypic and genotypic resistance data support the therapeutic evaluation of second-generation tetracyclines like MIN as promising agents for treating MDRAB infections... - A pilot study evaluating the safety of vaginal administration of a multi-particulate pellet formulationGuido Lopes Dos Santos Santiago
Laboratory of Bacteriology Research LBR, Ghent University, Ghent, Belgium
Eur J Pharm Biopharm 73:399-403. 2009..Quantitative evaluation of the effect caused by vaginal administration of gelatin capsules loaded with starch pellets and lyophilized powder, respectively, on vaginal pH and microflora... - Rapid genotyping of Achromobacter xylosoxidans, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates using melting curve analysis of RAPD-generated DNA fragments (McRAPD)Pieter Deschaght
Laboratory for Bacteriology Research LBR, Ghent University Hospital, University of Ghent, De Pintelaan 185, 9000 Ghent, Belgium
Res Microbiol 162:386-92. 2011..g. in case of suspicion of hospital outbreak, via a less costly, more rapid, less laborious and more user-friendly technique than RAPD followed by electrophoresis... - Array-based identification of species of the genera Abiotrophia, Enterococcus, Granulicatella, and StreptococcusSheng Kai Tung
Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China, and Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Belgium
J Clin Microbiol 44:4414-24. 2006..The oligonucleotide array is accurate for identification of the above microorganisms and could be used as a reliable alternative to phenotypic identification methods... - Achromobacter xylosoxidans in cystic fibrosis: prevalence and clinical relevanceFrans De Baets
Cystic Fibrosis Centre, University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium
J Cyst Fibros 6:75-8. 2007..Achromobacter xylosoxidans is increasingly cultured in sputum from cystic fibrosis (CF) patients; nevertheless, there are few published data on the clinical impact of this infection or chronic colonisation... - Gene polymorphisms of Toll-like and related recognition receptors in relation to the vaginal carriage of Gardnerella vaginalis and Atopobium vaginaeHans Verstraelen
Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185, B 9000 Ghent, Belgium
J Reprod Immunol 79:163-73. 2009..Our data suggest that some degree of genetic susceptibility involving pathogen recognition may occur with the key bacterial vaginosis organism, A. vaginae... - Susceptibility testing of Atopobium vaginae for dequalinium chlorideGuido Lopes dos Santos Santiago
Laboratory Bacteriology Research, Faculty Medicine and Health Sciences, University of Ghent, Ghent, Belgium
BMC Res Notes 5:151. 2012..We aimed to determine the MIC and MBC range of the broad-spectrum anti-infective and antiseptic dequalinium chloride for 28 strains, belonging to 4 species of the genus Atopobium, i.e. A. vaginae, A. minutum, A. rimae and A. parvulum... - An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systemsThierry de Baere
Department of Clinical Chemistry, Microbiology and Immunology, University Hospital Ghent, Belgium
BMC Microbiol 5:14. 2005..capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca.) capillary electrophoresis system... - Isolation of Buttiauxella gaviniae from a spinal cord patient with urinary bladder pathologyThierry de Baere
Laboratory Bacteriology and Virology, Department of Microbiology, Blok A, Ghent University Hospital, De Pintelaan 185, B9000 Ghent, Belgium
J Clin Microbiol 40:3867-70. 2002..6, and 136.9 bp and most resembled the tRNA-PCR pattern obtained for B. gaviniae, but they lacked the B. gaviniae fragments of 88.2 and 239.5 bp. To our knowledge, no clinical cases with Buttiauxella strains have been described thus far... - Quality-controlled small-scale production of a well-defined bacteriophage cocktail for use in human clinical trialsMaya Merabishvili
Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
PLoS ONE 4:e4944. 2009..The bacteriophage cocktail is currently being evaluated in a pilot clinical study cleared by a leading Medical Ethical Committee... - Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidaseGuido Lopes Dos Santos Santiago
Department of Clinical Chemistry, Microbiology, and Immunology, Laboratory for Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Belgium
Am J Obstet Gynecol 204:450.e1-7. 2011..Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase... - Elevated soluble triggering receptor expressed on myeloid cells (sTREM)-1 levels in maternal serum during term and preterm laborInge Tency
Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
PLoS ONE 8:e56050. 2013..Few studies have investigated serum sTREM-1 expression during preterm labor. Therefore, the purpose of this study was to assess sTREM-1 concentrations in maternal serum during term and preterm labor... - Herpes simplex virus type 1 infection facilitates invasion of Staphylococcus aureus into the nasal mucosa and nasal polyp tissueXiangdong Wang
Upper Airways Research Laboratory, Department of Oto Rhino Laryngology, Ghent University Hospital, Ghent, Belgium
PLoS ONE 7:e39875. 2012..aureus are not clearly understood. The aim of this study was to investigate the interaction between S. aureus and herpes simplex virus type 1 (HSV1) in the invasion of the nasal mucosa and nasal polyp tissue... - Psychrobacter isolates of human origin, other than Psychrobacter phenylpyruvicus, are predominantly Psychrobacter faecalis and Psychrobacter pulmonis, with emended description of P. faecalisPieter Deschaght
Laboratory Bacteriology Research LBR, Department Clinical Chemistry, Microbiology, Immunology, University of Ghent, Ghent, Belgium
Int J Syst Evol Microbiol 62:671-4. 2012..immobilis indicates that most of these human isolates belong to the species Psychrobacter faecalis and Psychrobacter pulmonis... - Pneumococcal conjugate vaccination in children with recurrent acute otitis media: a therapeutic alternative?Muriel J P van Kempen
Department of Otorhinolaryngology, Ghent University Hospital, Belgium
Int J Pediatr Otorhinolaryngol 70:275-85. 2006..Based on two clinical trials in healthy infants the American Academy of Pediatrics (AAP) advices immunization with a 7-valent pneumococcal conjugate vaccine in children with recurrent acute otitis media (AOM)... - Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratoryThierry de Baere
Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
BMC Microbiol 2:4. 2002..identification of all mycobacterial species at once, instead of attempting direct PCR based detection from clinical samples of M. tuberculosis only... - Terminal restriction fragment length polymorphism analysis of the microflora in necrotic teeth of patients irradiated in the head and neck regionGeert M G Hommez
Department of Restorative Dentistry and Endodontology, Ghent University Hospital, Gent, Belgium
J Endod 34:1048-51. 2008..Capnocytophaga spp., and Actinomyces spp.) found in group A were not found in group B. In conclusion, the diversity of root canal microflora increases significantly after head-neck radiotherapy... - PCR and the detection of Pseudomonas aeruginosa in respiratory samples of CF patients. A literature reviewPieter Deschaght
Laboratory Bacteriology Research, University of Ghent, De Pintelaan 185, 9000 Ghent, Belgium
J Cyst Fibros 10:293-7. 2011..The importance of choice of DNA-extraction methods and PCR formats and of the validation of their specificity and sensitivity with clinical samples is stressed... - Imbalances between matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in maternal serum during preterm laborInge Tency
Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
PLoS ONE 7:e49042. 2012..Therefore, the purpose of this study was to determine serum concentrations of MMP-3, MMP-9 and all four TIMPs as well as MMP:TIMP ratios during term and preterm labor... - The epidemiology of bacterial vaginosis in relation to sexual behaviourHans Verstraelen
Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
BMC Infect Dis 10:81. 2010..We therefore made a critical appraisal of the literature on BV in relation to sexual behaviour... - Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytesThierry de Baere
Laboratory for Bacteriology Research, University of Ghent, Ghent, Belgium
J Med Microbiol 59:48-54. 2010.... - Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogensEllen Decat
Biomedical and Exact Sciences, Faculty of Education, Health and Social Work, University College Ghent, Keramiekstraat 80, Ghent, Belgium
BMC Res Notes 5:664. 2012..e. Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Parvimonas micros, Porphyromonas gingivalis, Prevotella intermedia, Tanerella forsythia and Treponema denticola, and of the caries pathogen Streptococcus mutans... - Antibiotic susceptibility of acute otitis media pathogens in otitis-prone Belgian childrenMuriel J P van Kempen
Department of Otorhinolaryngology, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium
Eur J Pediatr 163:524-9. 2004..If antimicrobial therapy in this group of children is indicated, high dose amoxicillin is recommended whereas the use of macrolides is obsolete... - Group A streptococcal vaginitis: an unrecognized cause of vaginal symptoms in adult womenHans Verstraelen
Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
Arch Gynecol Obstet 284:95-8. 2011..We sought to give an overview of the epidemiology, risk factors, symptoms, signs, and treatment of GAS vaginitis in adult women... - Detection of enterotoxin DNA in Staphylococcus aureus strains obtained from the middle meatus in controls and nasal polyp patientsThibaut Van Zele
Ear, Nose, and Throat Department, Upper Airways Research Laboratory, Ghent University Hospital, Ghent, Belgium
Am J Rhinol 22:223-7. 2008..The purpose of this study was to investigate the presence of S. aureus enterotoxin genes and the agr subtype in bacterial DNA of S. aureus strains isolated from the middle meatus of nasal polyp and control patients... - Recovery of Moraxella ovis from the bovine respiratory tract and differentiation of Moraxella species by tDNA-intergenic spacer PCRBoudewijn Catry
Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B 9820 Merelbeke, Belgium
Vet Microbiol 120:375-80. 2007..25 for ceftiofur; 0.5 for oxytetracycline; 8 for gentamicin; 64 for spectinomycin; 0.5/9.5 for the combination trimethoprim-sulfonamides; 4 for erythromycin; 8 for tilmicosin; 1 for florfenicol and 0.125 for enrofloxacin... - Antimicrobial resistance and resistance genes in Staphylococcus aureus strains from rabbitsDieter Vancraeynest
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
Vet Microbiol 101:245-51. 2004..This study demonstrates that resistance to antimicrobial agents in S. aureus isolates originating from rabbits is relatively rare compared to resistance in S. aureus isolates originating from other animals and humans... - Selection and characterization of a candidate therapeutic bacteriophage that lyses the Escherichia coli O104:H4 strain from the 2011 outbreak in GermanyMaia Merabishvili
Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium
PLoS ONE 7:e52709. 2012..It is argued that phage therapy should be more considered as a potential armament against the growing threat of (resistant) bacterial infections... - Application of the antibiotic batumin for accurate and rapid identification of staphylococcal small colony variantsLarisa N Churkina
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
BMC Res Notes 5:374. 2012..whereas all other species tested thus far are batumin resistant. We assessed the efficacy of the batumin diagnostic preparation to identify staphylococcal small colony variants, isolated from osteomyelitis patients... - Susceptibility testing of fluconazole by the NCCLS broth macrodilution method, E-test, and disk diffusion for application in the routine laboratoryInge Vandenbossche
Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
J Clin Microbiol 40:918-21. 2002..The Rosco tablet showed the best agreement with the NCCLS reference method, especially when newly established breakpoints of R < or = 10 mm and S > or = 21 mm were used... - Beta-casein-derived peptides, produced by bacteria, stimulate cancer cell invasion and motilityMaria Jose Oliveira
Laboratory of Experimental Cancerology, Gent University Hospital, De Pintelaan 185, Gent, Belgium
EMBO J 22:6161-73. 2003..Delta opioid receptor (deltaOR) is a candidate receptor for the 13mer peptide since naloxone, an deltaOR antagonist, blocks both deltaOR serine phosphorylation and 13mer peptide-mediated invasion... - Identification of Lactobacillus species using tDNA-PCRMargo Baele
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
J Microbiol Methods 50:263-71. 2002..gallinarum and L. helveticus). Inclusion of the resulting fingerprints in a numerical database containing fingerprints of numerous other Gram-positive and Gram-negative species makes the identification of unknown strains possible... - European regulatory conundrum of phage therapyGilbert Verbeken
Hospital Center of the Base Queen Astrid, Laboratory for Molecular and Cellular Technology, Burn Unit, Bruynstraat 1, B 1120 Neder over Heembeek, Belgium
Future Microbiol 2:485-91. 2007..As a long-term solution, however, we suggest the creation of a specific section for phage therapy under the Advanced Therapy Medicinal Product Regulation... - Oligonucleotide array-based identification of species in the Acinetobacter calcoaceticus-A. baumannii complex in isolates from blood cultures and antimicrobial susceptibility testing of the isolatesWen Chien Ko
Department of Internal Medicine, Center of Infection Control, National Cheng Kung University Hospital, Tainan, Taiwan
J Clin Microbiol 46:2052-9. 2008..calcoaceticus-A. baumannii complex emphasize that the differentiation of species within the complex is relevant from a clinical-epidemiological point of view... - A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri groupSrdjan Stepanovic
Department of Bacteriology, School of Medicine, Institute of Microbiology and Immunology, Dr Subotica 1, 11000 Belgrade, Serbia
Syst Appl Microbiol 28:353-7. 2005..5-100% correlation). The accuracy and ease of use of this protocol suggest that it may be useful and valuable in microbiological laboratories for the identification of members of this group... - Emended description of Streptococcus ferus isolated from pigs and ratsMargo Baele
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
Int J Syst Evol Microbiol 53:143-6. 2003..In all these tests, the type strain of S. ferus (LMG 16520T), from a rat, was included. The results of the tests are described and an emended species description is presented... - Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimensAlexandr Nemec
National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic
Int J Syst Evol Microbiol 53:1563-7. 2003.... - Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratoryGeorges Wauters
Microbiology Unit, University of Louvain, UCL 5490, av Hippocrate 54, B 1200 Brussels, Belgium
J Clin Microbiol 43:2624-8. 2005..Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme... - Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer regionHsien Chang Chang
Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan
J Clin Microbiol 43:1632-9. 2005..In conclusion, ITS sequence-based identification is reliable and provides a promising tool for elucidation of the clinical significance of the different species of the A. calcoaceticus-A. baumannii complex... - Description of Comamonas aquatica comb. nov. and Comamonas kerstersii sp. nov. for two subgroups of Comamonas terrigena and emended description of Comamonas terrigenaGeorges Wauters
Medical Microbiology Unit, University of Louvain, Avenue Hippocrate 54, B-1200 Brussels, Belgium
Int J Syst Evol Microbiol 53:859-62. 2003..terrigena DNA group 2 [containing former Aquaspirillum aquaticum and E. Falsen (EF) group 10 strains] as Comamonas aquatica sp. nov., and C. terrigena DNA group 3 (containing former EF group 10 strains) as Comamonas kerstersii sp. nov... - Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animalsLuc A Devriese
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium
Int J Syst Evol Microbiol 55:1569-73. 2005..The type strain, LMG 22219(T) (=ON 86(T)=CCUG 49543(T)), was isolated from lung tissue of a cat... - Evaluation of tRNA gene PCR for identification of mollicutesTim Stakenborg
Veterinary and Agrochemical Research Center, Brussels, Belgium
J Clin Microbiol 43:4558-66. 2005..In conclusion, we found tRNA gene PCR to be a rapid and discriminatory method to correctly identify a large collection of different species of the class of Mollicutes and to recognize not-yet-described groups... - Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast speciesCaroline Massonet
Department of Immunology and Microbiology, Rega Institute, U Z Gasthuisberg, B 3000 Leuven, Belgium
J Clin Microbiol 42:2209-11. 2004..ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly... - Description of Wautersiella falsenii gen. nov., sp. nov., to accommodate clinical isolates phenotypically resembling members of the genera Chryseobacterium and EmpedobacterPeter Kampfer
Institut fur Angewandte Mikrobiologie, Justus Liebig Universitat Giessen, Germany
Int J Syst Evol Microbiol 56:2323-9. 2006..The reference strain for genomovar 2 is NF 770 (=CCUG 51537=CIP 108860), which was isolated from blood... - Identification of species of Abiotrophia, Enterococcus, Granulicatella and Streptococcus by sequence analysis of the ribosomal 16S-23S intergenic spacer regionSheng Kai Tung
Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan, ROC
J Med Microbiol 56:504-13. 2007..In conclusion, ITS sequencing provides a useful approach towards identifying this group of pathogens on a molecular platform alongside 16S rRNA gene sequencing... - Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCRBurhan Cetinkaya
Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey
Vet Microbiol 88:75-83. 2002.... - Active Crohn's disease and ulcerative colitis can be specifically diagnosed and monitored based on the biostructure of the fecal floraAlexander Swidsinski
Humboldt University, Charite Hospital, Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms, Berlin, Germany
Inflamm Bowel Dis 14:147-61. 2008..The intestinal microflora is important in the pathogenesis of inflammatory bowel disease (IBD). The impact of its spatial organization on health and disease is unknown... - Exogenous glutathione completes the defense against oxidative stress in Haemophilus influenzaeBjorn Vergauwen
Laboratory for Protein Biochemistry and Protein Engineering, Ghent University, Belgium
J Bacteriol 185:1572-81. 2003..coli. Finally, we demonstrated that cysteine is an essential amino acid for growth and that cystine, GSH, glutathione amide, and cysteinylglycine can be catabolized in order to complement cysteine deficiency...