Mario Vaneechoutte

Summary

Affiliation: Ghent University
Country: Belgium

Publications

  1. pmc The vaginal microflora in relation to gingivitis
    Rutger Persson
    Department of Periodontology, Div of Oral Microbiology, University of Berne, Berne, Switzerland
    BMC Infect Dis 9:6. 2009
  2. pmc Application of the antibiotic batumin for accurate and rapid identification of staphylococcal small colony variants
    Larisa N Churkina
    Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
    BMC Res Notes 5:374. 2012
  3. pmc Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp
    Antonio Machado
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710 057, Portugal
    BMC Microbiol 13:82. 2013
  4. doi Description of Acidovorax wautersii sp. nov. to accommodate clinical isolates and an environmental isolate, most closely related to Acidovorax avenae
    Mario Vaneechoutte
    Laboratory Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Int J Syst Evol Microbiol 63:2203-6. 2013
  5. pmc Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species
    Tim Stakenborg
    Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium
    BMC Infect Dis 5:46. 2005
  6. pmc Exploring the evolutionary dynamics of plasmids: the Acinetobacter pan-plasmidome
    Marco Fondi
    Laboratory of Microbial and Molecular Evolution, Dept, of Evolutionary Biology, via Romana 17 19, University of Florence, I 50125 Florence, Italy
    BMC Evol Biol 10:59. 2010
  7. doi From the primordial soup to the latest universal common ancestor
    Mario Vaneechoutte
    Laboratory Bacteriology Research, Faculty Medicine and Health Sciences, University of Ghent, 3 Blok A, De Pintelaan 185, 9000 Gent, Belgium
    Res Microbiol 160:437-40. 2009
  8. pmc Naturally transformable Acinetobacter sp. strain ADP1 belongs to the newly described species Acinetobacter baylyi
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Blok A, De Pintelaan 185, 9000 Gent, Belgium
    Appl Environ Microbiol 72:932-6. 2006
  9. doi Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp. nov
    Mario Vaneechoutte
    Laboratory Bacteriology Research LBR, Department of Clinical Chemistry, Ghent University Hospital, 9000 Gent, Belgium
    Int J Syst Evol Microbiol 59:1376-81. 2009
  10. ncbi Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    Int J Syst Evol Microbiol 57:2623-8. 2007

Detail Information

Publications92

  1. pmc The vaginal microflora in relation to gingivitis
    Rutger Persson
    Department of Periodontology, Div of Oral Microbiology, University of Berne, Berne, Switzerland
    BMC Infect Dis 9:6. 2009
    ..Gingivitis has been linked to adverse pregnancy outcome (APO). Bacterial vaginosis (BV) has been associated with APO. We assessed if bacterial counts in BV is associated with gingivitis suggesting a systemic infectious susceptibilty...
  2. pmc Application of the antibiotic batumin for accurate and rapid identification of staphylococcal small colony variants
    Larisa N Churkina
    Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
    BMC Res Notes 5:374. 2012
    ..whereas all other species tested thus far are batumin resistant. We assessed the efficacy of the batumin diagnostic preparation to identify staphylococcal small colony variants, isolated from osteomyelitis patients...
  3. pmc Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp
    Antonio Machado
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710 057, Portugal
    BMC Microbiol 13:82. 2013
    ..Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluorescence in situ Hybridization assay (PNA FISH) for the detection of Lactobacillus spp. and G. vaginalis in mixed samples...
  4. doi Description of Acidovorax wautersii sp. nov. to accommodate clinical isolates and an environmental isolate, most closely related to Acidovorax avenae
    Mario Vaneechoutte
    Laboratory Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Int J Syst Evol Microbiol 63:2203-6. 2013
    ..nov. is proposed. The type strain is NF 1078(T) (=LMG 26971(T)=CCUG 62584(T))...
  5. pmc Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species
    Tim Stakenborg
    Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium
    BMC Infect Dis 5:46. 2005
    ..We studied the applicability of amplified rDNA restriction analysis (ARDRA) for the identification of all 116 acknowledged Mycoplasma species and subspecies...
  6. pmc Exploring the evolutionary dynamics of plasmids: the Acinetobacter pan-plasmidome
    Marco Fondi
    Laboratory of Microbial and Molecular Evolution, Dept, of Evolutionary Biology, via Romana 17 19, University of Florence, I 50125 Florence, Italy
    BMC Evol Biol 10:59. 2010
    ..Given their importance for conferring adaptive traits to the host organisms, the interest in plasmid sequencing is growing and now many complete plasmid sequences are available online...
  7. doi From the primordial soup to the latest universal common ancestor
    Mario Vaneechoutte
    Laboratory Bacteriology Research, Faculty Medicine and Health Sciences, University of Ghent, 3 Blok A, De Pintelaan 185, 9000 Gent, Belgium
    Res Microbiol 160:437-40. 2009
    ....
  8. pmc Naturally transformable Acinetobacter sp. strain ADP1 belongs to the newly described species Acinetobacter baylyi
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Blok A, De Pintelaan 185, 9000 Gent, Belgium
    Appl Environ Microbiol 72:932-6. 2006
    ....
  9. doi Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp. nov
    Mario Vaneechoutte
    Laboratory Bacteriology Research LBR, Department of Clinical Chemistry, Ghent University Hospital, 9000 Gent, Belgium
    Int J Syst Evol Microbiol 59:1376-81. 2009
    ..The five strains represent a novel species for which the name Acinetobacter venetianus sp. nov. is proposed. The type strain is RAG-1(T) (=ATCC 31012(T)=CCUG 45561(T)=LMG 19082(T)=LUH 3904(T)=NIPH 1925(T))...
  10. ncbi Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    Int J Syst Evol Microbiol 57:2623-8. 2007
    ..hispanicum. The DNA G+C content of strain NF802T is 36.5 mol%. The name Chryseobacterium hominis sp. nov. is proposed for this taxon, with type strain NF802T (=CCUG 52711T=CIP 109415T)...
  11. doi Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, University Hospital, Blok A, B 9000 Gent, Belgium
    Int J Syst Evol Microbiol 58:937-40. 2008
    ..These findings were corroborated for a second representative of the A. grimontii type strain, DSM 14968T. These data confirm that Acinetobacter grimontii is a later heterotypic synonym of Acinetobacter junii...
  12. ncbi Digitized fluorescent RFLP analysis (fRFLP) as a universal method for comparing genomes of culturable dsDNA viruses: application to bacteriophages
    Maia Merabishvili
    George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
    Res Microbiol 158:572-81. 2007
    ....
  13. ncbi Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, Blok A, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
    Int J Syst Evol Microbiol 54:317-27. 2004
    ..eutropha lineage should be classified in a novel genus, Wautersia gen. nov. Finally, based on the literature and new DNA-DNA hybridization data, it is proposed that Pseudomonas syzygii should be renamed Ralstonia syzygii comb. nov...
  14. pmc Longitudinal qPCR study of the dynamics of L. crispatus, L. iners, A. vaginae, (sialidase positive) G. vaginalis, and P. bivia in the vagina
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 7:e45281. 2012
    ..To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles...
  15. pmc Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women
    Nabil A El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 10:285. 2010
    ..However, this procedure may require 48 h to complete. We compared different sampling and culture techniques for the detection of GBS...
  16. pmc Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cycles
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 6:e28180. 2011
    ....
  17. pmc Genotyping of Streptococcus agalactiae (group B streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancy
    Nabil Abdullah El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 9:153. 2009
    ..Here we compared different culture media for GBS detection and we compared the occurrence of different genotypes and serotypes of GBS isolates from the vagina and rectum...
  18. pmc Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora
    Nabil Abdullah El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 9:167. 2009
    ..The vaginal microflora is important for maintaining vaginal health and preventing infections of the reproductive tract. The rectum has been suggested as the major source for the colonisation of the vaginal econiche...
  19. doi Comparison of culture with two different qPCR assays for detection of rectovaginal carriage of Streptococcus agalactiae (group B streptococci) in pregnant women
    Nabil Abdullah El Aila
    Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, 3BlokA, 185 De Pintelaan, 9000 Ghent, Flanders, Belgium
    Res Microbiol 162:499-505. 2011
    ..qPCR after Lim broth enrichment further increased the percentage of GBS-positive women, as detected by direct qPCR, from 27 to 33%, although the bacterial inoculum was low for these subjects...
  20. doi In vivo evaluation of the vaginal distribution and retention of a multi-particulate pellet formulation
    Nele Poelvoorde
    Laboratory of Pharmaceutical Technology, Ghent University, Ghent 9000, Belgium
    Eur J Pharm Biopharm 73:280-4. 2009
    ..These results were confirmed by swabbing to determine the amount of riboflavin sodium phosphate (used as marker) distributed in the different vaginal regions...
  21. pmc Bacteremic infection with Pantoea ananatis
    Thierry de Baere
    Department of Clinical Chemistry, Microbiology and Immunology, Blok A, University Hospital, Ghent, Belgium thierry debaire ugent be
    J Clin Microbiol 42:4393-5. 2004
    ..Subsequent sequencing of the 16S rRNA gene revealed a final identification as Pantoea ananatis. The patient was given intravenous and oral therapy with piperacillin-tazobactam and ofloxacin and recovered completely from his infection...
  22. ncbi Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals
    Luc A Devriese
    Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
    Int J Syst Evol Microbiol 55:1569-73. 2005
    ..The type strain, LMG 22219(T) (=ON 86(T)=CCUG 49543(T)), was isolated from lung tissue of a cat...
  23. ncbi Study of the intra- and interlaboratory reproducibility of partial single base C-sequencing of the 16S rRNA gene and its applicability for the identification of members of the genus Streptococcus
    Virginie Storms
    Department of Biochemistry, Physiology and Microbiology, Faculty of Sciences, University of Ghent, Gent, Belgium
    Syst Appl Microbiol 25:52-9. 2002
    ....
  24. doi Strong correspondence in bacterial loads between the vagina and rectum of pregnant women
    Nabil Abdullah El Aila
    Laboratory of Bacteriology Research, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, 3BlokA, 185 De Pintelaan, 9000 Ghent, Flanders, Belgium
    Res Microbiol 162:506-13. 2011
    ....
  25. pmc Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients
    Pieter Deschaght
    Laboratory for Bacteriology Research, Ghent University Hospital, University of Ghent, Ghent, Belgium
    BMC Microbiol 9:244. 2009
    ..aeruginosa oprL gene. In addition, we compared five DNA-extraction protocols...
  26. pmc Antibiotic susceptibility of Atopobium vaginae
    Ellen De Backer
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Infect Dis 6:51. 2006
    ..Thus far the four isolates of this fastidious micro-organism were found to be highly resistant to metronidazole and susceptible for clindamycin, two antibiotics preferred for the treatment of BV...
  27. pmc Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microflora
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
    BMC Microbiol 5:61. 2005
    ....
  28. pmc Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners
    Ellen De Backer
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
    BMC Microbiol 7:115. 2007
    ..iners and L. jensenii to obtain a quantitative non culture-based determination of these species in 71 vaginal samples from 32 pregnant and 28 non-pregnant women aged between 18 and 45 years...
  29. doi tDNA-PCR followed by automated fluorescent capillary electrophoresis for identification of bacterial species
    Margo Baele
    Laboratory for Veterinary Microbiology, Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, 9000 Gent, Belgium
    Cold Spring Harb Protoc 2009:pdb.prot5196. 2009
    ..g., after 16S rRNA gene sequence determination) can be added to expand the database further. This protocol describes tDNA-PCR, followed by automated fluorescent capillary electrophoresis to identify bacterial species...
  30. ncbi Repetitive-DNA-element PCR fingerprinting and antibiotic resistance of pan-European multi-resistant Acinetobacter baumannii clone III strains
    Geert Huys
    Laboratory of Microbiology and BCCM LMG Bacteria Collection, Ghent University, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    J Med Microbiol 54:851-6. 2005
    ....
  31. doi Genotype based evaluation of Pseudomonas aeruginosa eradication treatment success in cystic fibrosis patients
    Petra Schelstraete
    Cystic Fibrosis Center Gent, Ghent University Hospital, 9000 Gent, Belgium
    J Cyst Fibros 9:99-103. 2010
    ..We followed cystic fibrosis patients after a first ever isolation of P. aeruginosa and evaluated the P. aeruginosa-free time period after eradication therapy...
  32. pmc The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays
    Nabil Abdullah El Aila
    Laboratory Bacteriology Research, Department of Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium
    BMC Infect Dis 10:104. 2010
    ..Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge...
  33. ncbi Distribution of tetracycline resistance genes in genotypically related and unrelated multiresistant Acinetobacter baumannii strains from different European hospitals
    Geert Huys
    Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, Ghent, Belgium
    Res Microbiol 156:348-55. 2005
    ..Collectively, our phenotypic and genotypic resistance data support the therapeutic evaluation of second-generation tetracyclines like MIN as promising agents for treating MDRAB infections...
  34. ncbi Emended description of Streptococcus ferus isolated from pigs and rats
    Margo Baele
    Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
    Int J Syst Evol Microbiol 53:143-6. 2003
    ..In all these tests, the type strain of S. ferus (LMG 16520T), from a rat, was included. The results of the tests are described and an emended species description is presented...
  35. pmc Shared genotypes of Achromobacter xylosoxidans strains isolated from patients at a cystic fibrosis rehabilitation center
    Sabine Van Daele
    Department of Pediatric Pulmonology, University Hospital Ghent, De Pintelaan 185, B9000 Ghent, Belgium
    J Clin Microbiol 43:2998-3002. 2005
    ..aeruginosa, as well as two clusters of 4 and 10 patients, respectively, colonized with genotypically identical Achromobacter xylosoxidans strains, were discovered...
  36. pmc Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    BMC Microbiol 9:116. 2009
    ....
  37. pmc Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients
    Pieter Deschaght
    Laboratory for Bacteriology Research, Ghent University Hospital, Ghent University, Ghent, Belgium
    BMC Microbiol 10:245. 2010
    ..In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients...
  38. pmc Bacteremia due to Moraxella atlantae in a cancer patient
    Thierry de Baere
    Department of Microbiology, Ghent University Hospital, Ghent, Belgium Thierry De
    J Clin Microbiol 40:2693-5. 2002
    ..atlantae, M. lincolnii, and M. osloensis might constitute three separate genera within the MORAXELLACEAE: After treatment with amoxicillin-clavulanic acid for 2 days, fever subsided and the patient was dismissed...
  39. pmc Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2)
    Thierry de Baere
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Belgium
    BMC Microbiol 2:21. 2002
    ..Therefore, we evaluated the feasability of PCR-based amplification of the Internally Transcribed Spacer region 2, followed by fragment size analysis on the ABI Prism 310 for the identification of clinically important yeasts...
  40. ncbi Streptococcus cristatus isolated from a resected heart valve and blood cultures: case reports and application of phenotypic and genotypic techniques for identification
    Charlotte Matthys
    Dienst Klinische Chemie, Microbiologie en Immunologie, Universitair Ziekenhuis Gent, De Pintelaan 185, B 9000 Gent, Belgie
    Acta Clin Belg 61:196-200. 2006
    ..Using tDNA-PCR, it is possible to identify to species level and to differentiate between species of the viridans group...
  41. ncbi Recovery of Moraxella ovis from the bovine respiratory tract and differentiation of Moraxella species by tDNA-intergenic spacer PCR
    Boudewijn Catry
    Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B 9820 Merelbeke, Belgium
    Vet Microbiol 120:375-80. 2007
    ..25 for ceftiofur; 0.5 for oxytetracycline; 8 for gentamicin; 64 for spectinomycin; 0.5/9.5 for the combination trimethoprim-sulfonamides; 4 for erythromycin; 8 for tilmicosin; 1 for florfenicol and 0.125 for enrofloxacin...
  42. doi Gene polymorphisms of Toll-like and related recognition receptors in relation to the vaginal carriage of Gardnerella vaginalis and Atopobium vaginae
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185, B 9000 Ghent, Belgium
    J Reprod Immunol 79:163-73. 2009
    ..Our data suggest that some degree of genetic susceptibility involving pathogen recognition may occur with the key bacterial vaginosis organism, A. vaginae...
  43. ncbi Identification of Lactobacillus species using tDNA-PCR
    Margo Baele
    Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    J Microbiol Methods 50:263-71. 2002
    ..gallinarum and L. helveticus). Inclusion of the resulting fingerprints in a numerical database containing fingerprints of numerous other Gram-positive and Gram-negative species makes the identification of unknown strains possible...
  44. pmc Microflora of the penile skin-lined neovagina of transsexual women
    Steven Weyers
    Department of Obstetrics and Gynaecology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 9:102. 2009
    ..Secondary objectives were to describe possible correlations of this microflora with multiple patients' characteristics, such as sexual orientation, the incidence of vaginal irritation and malodorous vaginal discharge...
  45. pmc Comparison of five genotypic techniques for identification of optochin-resistant pneumococcus-like isolates
    Rita Verhelst
    Department of Chemistry, Microbiology and Immunology, Ghent University Hospital, Gent, Belgium
    J Clin Microbiol 41:3521-5. 2003
    ..We found the psaA-specific PCR to be the genotypic technique best suited for the identification of genuine pneumococci and optochin-resistant pneumococci...
  46. pmc Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 4:16. 2004
    ..Here, we report on the results obtained by combining culture and PCR-based methods to characterize the normal and disturbed vaginal microflora...
  47. pmc Extended-Spectrum beta-lactamase (ESBL) producing Enterobacter aerogenes phenotypically misidentified as Klebsiella pneumoniae or K. terrigena
    Geert Claeys
    Department of Microbiology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 4:49. 2004
    ..Here we report that genotypically unrelated strains of E. aerogenes can be misidentified as K. pneumoniae by routine laboratories using standard biochemical identification and using identification automates...
  48. doi A pilot study evaluating the safety of vaginal administration of a multi-particulate pellet formulation
    Guido Lopes Dos Santos Santiago
    Laboratory of Bacteriology Research LBR, Ghent University, Ghent, Belgium
    Eur J Pharm Biopharm 73:399-403. 2009
    ..Quantitative evaluation of the effect caused by vaginal administration of gelatin capsules loaded with starch pellets and lyophilized powder, respectively, on vaginal pH and microflora...
  49. doi Rapid genotyping of Achromobacter xylosoxidans, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates using melting curve analysis of RAPD-generated DNA fragments (McRAPD)
    Pieter Deschaght
    Laboratory for Bacteriology Research LBR, Ghent University Hospital, University of Ghent, De Pintelaan 185, 9000 Ghent, Belgium
    Res Microbiol 162:386-92. 2011
    ..g. in case of suspicion of hospital outbreak, via a less costly, more rapid, less laborious and more user-friendly technique than RAPD followed by electrophoresis...
  50. ncbi Modified classification of Gram-stained vaginal smears to predict spontaneous preterm birth: a prospective cohort study
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Am J Obstet Gynecol 196:528.e1-6. 2007
    ..The purpose of this study was to identify women at risk of spontaneous preterm birth through a 4-category Gram-stained vaginal smear method, not restricted to bacterial vaginosis...
  51. ncbi Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium
    Am J Obstet Gynecol 191:1130-2. 2004
    ..Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora...
  52. pmc Array-based identification of species of the genera Abiotrophia, Enterococcus, Granulicatella, and Streptococcus
    Sheng Kai Tung
    Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China, and Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Belgium
    J Clin Microbiol 44:4414-24. 2006
    ..The oligonucleotide array is accurate for identification of the above microorganisms and could be used as a reliable alternative to phenotypic identification methods...
  53. pmc Quality-controlled small-scale production of a well-defined bacteriophage cocktail for use in human clinical trials
    Maya Merabishvili
    Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
    PLoS ONE 4:e4944. 2009
    ..The bacteriophage cocktail is currently being evaluated in a pilot clinical study cleared by a leading Medical Ethical Committee...
  54. ncbi Achromobacter xylosoxidans in cystic fibrosis: prevalence and clinical relevance
    Frans De Baets
    Cystic Fibrosis Centre, University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium
    J Cyst Fibros 6:75-8. 2007
    ..Achromobacter xylosoxidans is increasingly cultured in sputum from cystic fibrosis (CF) patients; nevertheless, there are few published data on the clinical impact of this infection or chronic colonisation...
  55. pmc Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens
    Ellen Decat
    Biomedical and Exact Sciences, Faculty of Education, Health and Social Work, University College Ghent, Keramiekstraat 80, Ghent, Belgium
    BMC Res Notes 5:664. 2012
    ..e. Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Parvimonas micros, Porphyromonas gingivalis, Prevotella intermedia, Tanerella forsythia and Treponema denticola, and of the caries pathogen Streptococcus mutans...
  56. doi Psychrobacter isolates of human origin, other than Psychrobacter phenylpyruvicus, are predominantly Psychrobacter faecalis and Psychrobacter pulmonis, with emended description of P. faecalis
    Pieter Deschaght
    Laboratory Bacteriology Research LBR, Department Clinical Chemistry, Microbiology, Immunology, University of Ghent, Ghent, Belgium
    Int J Syst Evol Microbiol 62:671-4. 2012
    ..immobilis indicates that most of these human isolates belong to the species Psychrobacter faecalis and Psychrobacter pulmonis...
  57. doi Isolation and identification of Mycobacterium avium subspecies silvaticum from a horse
    Koen Chiers
    Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
    Comp Immunol Microbiol Infect Dis 35:303-7. 2012
    ..avium subsp. silvaticum in a three-year-old gelding presenting with caseous, necrotizing, granulomatous lesions. The result was confirmed by sequencing of the 85B antigen gene...
  58. pmc Stability of Staphylococcus aureus phage ISP after freeze-drying (lyophilization)
    Maia Merabishvili
    Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium
    PLoS ONE 8:e68797. 2013
    ..Sucrose and trehalose were shown to be the best stabilizing additives, causing a decrease of only 1 log immediately after the lyophilization procedure and showing high stability during a 27 month storage period...
  59. pmc Susceptibility testing of Atopobium vaginae for dequalinium chloride
    Guido Lopes dos Santos Santiago
    Laboratory Bacteriology Research, Faculty Medicine and Health Sciences, University of Ghent, Ghent, Belgium
    BMC Res Notes 5:151. 2012
    ..We aimed to determine the MIC and MBC range of the broad-spectrum anti-infective and antiseptic dequalinium chloride for 28 strains, belonging to 4 species of the genus Atopobium, i.e. A. vaginae, A. minutum, A. rimae and A. parvulum...
  60. pmc An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems
    Thierry de Baere
    Department of Clinical Chemistry, Microbiology and Immunology, University Hospital Ghent, Belgium
    BMC Microbiol 5:14. 2005
    ..capillary electrophoresis system with that obtained by a CEQ8000 (Beckman Coulter, Fullerton, Ca.) capillary electrophoresis system...
  61. pmc Isolation of Buttiauxella gaviniae from a spinal cord patient with urinary bladder pathology
    Thierry de Baere
    Laboratory Bacteriology and Virology, Department of Microbiology, Blok A, Ghent University Hospital, De Pintelaan 185, B9000 Ghent, Belgium
    J Clin Microbiol 40:3867-70. 2002
    ..6, and 136.9 bp and most resembled the tRNA-PCR pattern obtained for B. gaviniae, but they lacked the B. gaviniae fragments of 88.2 and 239.5 bp. To our knowledge, no clinical cases with Buttiauxella strains have been described thus far...
  62. doi Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase
    Guido Lopes Dos Santos Santiago
    Department of Clinical Chemistry, Microbiology, and Immunology, Laboratory for Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Belgium
    Am J Obstet Gynecol 204:450.e1-7. 2011
    ..Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase...
  63. ncbi Antimicrobial resistance and resistance genes in Staphylococcus aureus strains from rabbits
    Dieter Vancraeynest
    Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium
    Vet Microbiol 101:245-51. 2004
    ..This study demonstrates that resistance to antimicrobial agents in S. aureus isolates originating from rabbits is relatively rare compared to resistance in S. aureus isolates originating from other animals and humans...
  64. pmc Imbalances between matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in maternal serum during preterm labor
    Inge Tency
    Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 7:e49042. 2012
    ..Therefore, the purpose of this study was to determine serum concentrations of MMP-3, MMP-9 and all four TIMPs as well as MMP:TIMP ratios during term and preterm labor...
  65. pmc Elevated soluble triggering receptor expressed on myeloid cells (sTREM)-1 levels in maternal serum during term and preterm labor
    Inge Tency
    Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 8:e56050. 2013
    ..Few studies have investigated serum sTREM-1 expression during preterm labor. Therefore, the purpose of this study was to assess sTREM-1 concentrations in maternal serum during term and preterm labor...
  66. pmc Herpes simplex virus type 1 infection facilitates invasion of Staphylococcus aureus into the nasal mucosa and nasal polyp tissue
    Xiangdong Wang
    Upper Airways Research Laboratory, Department of Oto Rhino Laryngology, Ghent University Hospital, Ghent, Belgium
    PLoS ONE 7:e39875. 2012
    ..aureus are not clearly understood. The aim of this study was to investigate the interaction between S. aureus and herpes simplex virus type 1 (HSV1) in the invasion of the nasal mucosa and nasal polyp tissue...
  67. ncbi Pneumococcal conjugate vaccination in children with recurrent acute otitis media: a therapeutic alternative?
    Muriel J P van Kempen
    Department of Otorhinolaryngology, Ghent University Hospital, Belgium
    Int J Pediatr Otorhinolaryngol 70:275-85. 2006
    ..Based on two clinical trials in healthy infants the American Academy of Pediatrics (AAP) advices immunization with a 7-valent pneumococcal conjugate vaccine in children with recurrent acute otitis media (AOM)...
  68. pmc Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory
    Thierry de Baere
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 2:4. 2002
    ..identification of all mycobacterial species at once, instead of attempting direct PCR based detection from clinical samples of M. tuberculosis only...
  69. doi PCR and the detection of Pseudomonas aeruginosa in respiratory samples of CF patients. A literature review
    Pieter Deschaght
    Laboratory Bacteriology Research, University of Ghent, De Pintelaan 185, 9000 Ghent, Belgium
    J Cyst Fibros 10:293-7. 2011
    ..The importance of choice of DNA-extraction methods and PCR formats and of the validation of their specificity and sensitivity with clinical samples is stressed...
  70. doi Terminal restriction fragment length polymorphism analysis of the microflora in necrotic teeth of patients irradiated in the head and neck region
    Geert M G Hommez
    Department of Restorative Dentistry and Endodontology, Ghent University Hospital, Gent, Belgium
    J Endod 34:1048-51. 2008
    ..Capnocytophaga spp., and Actinomyces spp.) found in group A were not found in group B. In conclusion, the diversity of root canal microflora increases significantly after head-neck radiotherapy...
  71. pmc The epidemiology of bacterial vaginosis in relation to sexual behaviour
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    BMC Infect Dis 10:81. 2010
    ..We therefore made a critical appraisal of the literature on BV in relation to sexual behaviour...
  72. doi Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytes
    Thierry de Baere
    Laboratory for Bacteriology Research, University of Ghent, Ghent, Belgium
    J Med Microbiol 59:48-54. 2010
    ....
  73. ncbi Antibiotic susceptibility of acute otitis media pathogens in otitis-prone Belgian children
    Muriel J P van Kempen
    Department of Otorhinolaryngology, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium
    Eur J Pediatr 163:524-9. 2004
    ..Among nasopharyngeal and middle ear H. influenzae isolates, 17.1% were resistant to ampicillin and 16.3% to cotrimoxazole. For M. catarrhalis, 92.4% of all isolates was ampicillin-resistant...
  74. doi Group A streptococcal vaginitis: an unrecognized cause of vaginal symptoms in adult women
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Arch Gynecol Obstet 284:95-8. 2011
    ..We sought to give an overview of the epidemiology, risk factors, symptoms, signs, and treatment of GAS vaginitis in adult women...
  75. doi Detection of enterotoxin DNA in Staphylococcus aureus strains obtained from the middle meatus in controls and nasal polyp patients
    Thibaut Van Zele
    Ear, Nose, and Throat Department, Upper Airways Research Laboratory, Ghent University Hospital, Ghent, Belgium
    Am J Rhinol 22:223-7. 2008
    ..The purpose of this study was to investigate the presence of S. aureus enterotoxin genes and the agr subtype in bacterial DNA of S. aureus strains isolated from the middle meatus of nasal polyp and control patients...
  76. pmc Selection and characterization of a candidate therapeutic bacteriophage that lyses the Escherichia coli O104:H4 strain from the 2011 outbreak in Germany
    Maia Merabishvili
    Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium
    PLoS ONE 7:e52709. 2012
    ..It is argued that phage therapy should be more considered as a potential armament against the growing threat of (resistant) bacterial infections...
  77. pmc Beta-casein-derived peptides, produced by bacteria, stimulate cancer cell invasion and motility
    Maria Jose Oliveira
    Laboratory of Experimental Cancerology, Gent University Hospital, De Pintelaan 185, Gent, Belgium
    EMBO J 22:6161-73. 2003
    ..Delta opioid receptor (deltaOR) is a candidate receptor for the 13mer peptide since naloxone, an deltaOR antagonist, blocks both deltaOR serine phosphorylation and 13mer peptide-mediated invasion...
  78. pmc Susceptibility testing of fluconazole by the NCCLS broth macrodilution method, E-test, and disk diffusion for application in the routine laboratory
    Inge Vandenbossche
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    J Clin Microbiol 40:918-21. 2002
    ..The Rosco tablet showed the best agreement with the NCCLS reference method, especially when newly established breakpoints of R < or = 10 mm and S > or = 21 mm were used...
  79. pmc Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region
    Hsien Chang Chang
    Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan
    J Clin Microbiol 43:1632-9. 2005
    ..In conclusion, ITS sequence-based identification is reliable and provides a promising tool for elucidation of the clinical significance of the different species of the A. calcoaceticus-A. baumannii complex...
  80. ncbi Description of Wautersiella falsenii gen. nov., sp. nov., to accommodate clinical isolates phenotypically resembling members of the genera Chryseobacterium and Empedobacter
    Peter Kampfer
    Institut fur Angewandte Mikrobiologie, Justus Liebig Universitat Giessen, Germany
    Int J Syst Evol Microbiol 56:2323-9. 2006
    ..The reference strain for genomovar 2 is NF 770 (=CCUG 51537=CIP 108860), which was isolated from blood...
  81. ncbi European regulatory conundrum of phage therapy
    Gilbert Verbeken
    Hospital Center of the Base Queen Astrid, Laboratory for Molecular and Cellular Technology, Burn Unit, Bruynstraat 1, B 1120 Neder over Heembeek, Belgium
    Future Microbiol 2:485-91. 2007
    ..As a long-term solution, however, we suggest the creation of a specific section for phage therapy under the Advanced Therapy Medicinal Product Regulation...
  82. pmc Evaluation of tRNA gene PCR for identification of mollicutes
    Tim Stakenborg
    Veterinary and Agrochemical Research Center, Brussels, Belgium
    J Clin Microbiol 43:4558-66. 2005
    ..In conclusion, we found tRNA gene PCR to be a rapid and discriminatory method to correctly identify a large collection of different species of the class of Mollicutes and to recognize not-yet-described groups...
  83. ncbi A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group
    Srdjan Stepanovic
    Department of Bacteriology, School of Medicine, Institute of Microbiology and Immunology, Dr Subotica 1, 11000 Belgrade, Serbia
    Syst Appl Microbiol 28:353-7. 2005
    ..5-100% correlation). The accuracy and ease of use of this protocol suggest that it may be useful and valuable in microbiological laboratories for the identification of members of this group...
  84. ncbi Description of Comamonas aquatica comb. nov. and Comamonas kerstersii sp. nov. for two subgroups of Comamonas terrigena and emended description of Comamonas terrigena
    Georges Wauters
    Medical Microbiology Unit, University of Louvain, Avenue Hippocrate 54, B 1200 Brussels, Belgium
    Int J Syst Evol Microbiol 53:859-62. 2003
    ..terrigena DNA group 2 [containing former Aquaspirillum aquaticum and E. Falsen (EF) group 10 strains] as Comamonas aquatica sp. nov., and C. terrigena DNA group 3 (containing former EF group 10 strains) as Comamonas kerstersii sp. nov...
  85. pmc Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species
    Caroline Massonet
    Department of Immunology and Microbiology, Rega Institute, U Z Gasthuisberg, B 3000 Leuven, Belgium
    J Clin Microbiol 42:2209-11. 2004
    ..ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly...
  86. pmc Oligonucleotide array-based identification of species in the Acinetobacter calcoaceticus-A. baumannii complex in isolates from blood cultures and antimicrobial susceptibility testing of the isolates
    Wen Chien Ko
    Department of Internal Medicine, Center of Infection Control, National Cheng Kung University Hospital, Tainan, Taiwan
    J Clin Microbiol 46:2052-9. 2008
    ..calcoaceticus-A. baumannii complex emphasize that the differentiation of species within the complex is relevant from a clinical-epidemiological point of view...
  87. ncbi Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimens
    Alexandr Nemec
    National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic
    Int J Syst Evol Microbiol 53:1563-7. 2003
    ....
  88. pmc Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory
    Georges Wauters
    Microbiology Unit, University of Louvain, UCL 5490, av Hippocrate 54, B 1200 Brussels, Belgium
    J Clin Microbiol 43:2624-8. 2005
    ..Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme...
  89. ncbi Active Crohn's disease and ulcerative colitis can be specifically diagnosed and monitored based on the biostructure of the fecal flora
    Alexander Swidsinski
    Humboldt University, Charite Hospital, Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms, Berlin, Germany
    Inflamm Bowel Dis 14:147-61. 2008
    ..The intestinal microflora is important in the pathogenesis of inflammatory bowel disease (IBD). The impact of its spatial organization on health and disease is unknown...
  90. ncbi Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR
    Burhan Cetinkaya
    Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey
    Vet Microbiol 88:75-83. 2002
    ....
  91. ncbi Identification of species of Abiotrophia, Enterococcus, Granulicatella and Streptococcus by sequence analysis of the ribosomal 16S-23S intergenic spacer region
    Sheng Kai Tung
    Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan, ROC
    J Med Microbiol 56:504-13. 2007
    ..In conclusion, ITS sequencing provides a useful approach towards identifying this group of pathogens on a molecular platform alongside 16S rRNA gene sequencing...
  92. pmc Exogenous glutathione completes the defense against oxidative stress in Haemophilus influenzae
    Bjorn Vergauwen
    Laboratory for Protein Biochemistry and Protein Engineering, Ghent University, Belgium
    J Bacteriol 185:1572-81. 2003
    ..coli. Finally, we demonstrated that cysteine is an essential amino acid for growth and that cystine, GSH, glutathione amide, and cysteinylglycine can be catabolized in order to complement cysteine deficiency...